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1) What’s in the bottles (from yesterday)? - Describe (draw and write). - Why does this happen (use polar / non-polar )?. J. 2) Membrane model: Write as many observations you can possibly see. Considering the bubble lab: 1. Describe the soap film. - PowerPoint PPT Presentation
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J1) What’s in the bottles (from yesterday)? - Describe (draw and write).- Why does this happen (use polar / non-polar)?
2) Membrane model: Write as many observations you can possibly see.
Considering the bubble lab:1. Describe the soap film.2. Describe what happened when you used:A. Dry Pencil, B. Wet Pencil, C. Wet tube with pencil, D. Wet thread-ring.3. Which characteristics of the membrane were demonstrated? At least 3.
Demonstration: 3 beakers and food coloring…
1. Describe (draw + write):- What happens to the food
coloring?- What is the difference
between the beakers? 2. What could be the reason
for this difference?
Membrane Function
Part One: Diffusion of particles across the membrane.
Hydro-philic
Hydro-phobic
Water
Water
Solvent: Fluid Substance that is present in large amount (water, oil)Solute: Substance that is in small amount and mixed into the solvent. (food coloring, paint, sugar..)Solution: Solute mixed into solventConcentration: Amount of solute in unit volume of solvent.
Diffusion and OsmosisDiffusion
•Particles move randomly as fast as the temperature allows.
Concentration: Amount of particles per unit volume
Solvent (water)
Solute (sugar, salt, etc)
Notes
Diffusion Simulation
Solution
Diffusion Simulation, Circles, Temperature
Particles move from high to low concentrations
Diffusion Simulation
Diffusion Open Memb
Example:
(Particles move down the concentration gradient).
Notes
Diffusion Animation Stolaf
Two eggs two solutions:
The eggs you are looking at were de-shelled with vinegar. They were then dipped overnight – one in syrup and one in water.
1.Draw what you see. Describe.2.Where to did the water move?3.Which egg was dipped in
syrup/water? How can you tell?
We divide all the particles to four major groups:
UnchargedPolar
Charged(“super-polar”)
+ -
Large(proteins)
Non-polar (lipids)
Pass freely:
H2O IonsNa+ Cl-
Need help to pass:
Biological membranes are
semi-permeablePass easily:
-Small molecules-Non-charged particles
-WATER!
Pass only with help: -Large particles
-Ions ?
Notes
+-
Osmosis
Osmosis Simulation
* Osmosis is the movement of water through a semi-permeable membrane.
Osmosis Semipermeable Memb
* Water moves (diffuses) until the concentrations are balanced.
Notes
Osmosis Animation - before and After salt
Handout exercise
Before the journal…
Handout: All three situations are unstable. On the right side draw in the particles and the water level after time passed for diffusion/osmosis to occur.
Two eggs two solutions:
The eggs you are looking at were de-shelled with vinegar. They were then dipped overnight – one in syrup and one in water.
1.Draw what you see. Describe.2.Where to did the water move?3.Which egg was dipped in
syrup/water? How can you tell?
1) Complete the drawing on the right of the cup:Since sugar cannot pass through, the water can and will pass. But to what direction?
2) Describe in words what happened to the water, and why.
Sugar Water
Isotonic – solutions with equal concentration.
When comparing two solutions to one another, we define:
A. B.
Osmosis Simulation
Hypotonic solution has a lower solute concentration
Hypertonic solution has a higher solute concentrations
In osmosis water moves from a hypo-tonic solution to a hyper-tonic solution
A. B. Water movement
1) Add the new terms to the drawing of the cup.
2) Back to the egg….:Draw the egg that was dipped in
syrup right after it was dipped.Label it – where was it hypo-,
hyper- or iso- tonic?3) Draw the egg after staying
overnight in syrup- label hypo-, hyper-, iso- tonic.
Diffusion Simulation
Diffusion Open Memb
Osmosis (New) No Cup
Osmosis Semipermeable Memb
Osmosis Animation - before and After salt (Stolaf)
Diffusion Simulation, Circles, Temperature
YouTube of a clear Osmosis Animation
Diffusion, Large, No cup
Hypertonic
H2O
Egg in Syrup
isotonic
Hypotonic
H2O
Egg in Syrup
isotonic
Hypotonic
Hypertonic
H2O
Egg in Water
isotonic
H2O
Egg in Water
Writing exercise:Draw the 3rd example from the handout. • Label: Hypertonic, Isotonic, Hypotonic, semi-permeable membrane, concentration gradient.• In essay format: Tell what happened in the experiment, why.• Peer (neighbor) review.
1)How would you prefer to store a salad – with or
without dressing? Why?
2) What happens when you add sugar to strawberries? Why does it happen? (use “Osmosis”)
And why are we learning this??To Explain:•Why can we safely store jam at room temperature.•Why do marine fish die in freshwater, and visa versa?•How can we desalinate sea water?•Why better store salad without dressing? And more…..
Quiz
Preparing for the Dialysis Lab
Dialysis – Filtration of liquids through an artificial membrane (bag).
Materials that will be added:Sugar ( ), Starch ( ….)Iodine ( ) Water ( )
1.Which will pass through the holes ?
2. How can we tell if they passed or not?
Observe the dialysis tubing from yesterday’s lab.
1) Describe (Draw, write) what you see, as detailed as possible:
2) Which materials passed through, in what direction, and how could you tell?
3) Copy the following data chart..
DATA TABLE Before After
Water: Volume changes in the ‘cell’
_______________________________ml
Iodine staining inside the ‘cell’ (Yes/No)
Iodine color outside the ‘cell’ (Yes/No)
Starch outside the ‘cell’
Sugar outside the ‘cell’: (Color; Yes/No)
Color: Yes/No
Color: Yes/No
Kidney tutorial and animation
A Youtube about the kidneys
Explain the following processes:Wilting of salads when stored in
dressing
• Putting an animal cell in a hypo-tonic solution? –What would happen?
B. Putting an animal cell in a hyper-tonic solution? What would happen?
Collapse of Blood Cell
Rupture of a blood cell
C. Putting a plant cell in a hypo-tonic solution? What would happen?
Why are concentrated jams or juices safe to store without cooling?
Or… Which of the following must you store in the refrigerator? Vinegar, jam, flour, candy, meat, diluted juice, concentrated juice,…Why?