$394 Thursday, November 10, 2005 Poster Abstracts

raised CRP levels had raised lipid levels. Levels of CRP correlated significantly with abnormal lipid levels. Conclusion: Raised CRP levels with its significant correlation with abnormal lipids may be an important factor in cerebrovascular arteriosclerosis leading to Ischaemfc Stroke.

1151 Functional evaluation of Stroke patients in Chile: three years follow up

Diaz V ~, Rodriguez C ~, Behrens MI ~. ~Departamento Neurologla y Neurocirugia, Universidad de Chile, Chile

Objective: To evaluate the functional status of ischenffc or haemor- rhagic stroke patients followed for three years. Design: Prospective cohort study of the functional status of all consecutive stroke inpatients from University of Chile Clinical Hospital between 1990 and 2001. Alive patients were visited and evaluated once a year. Death certificates were reviewed. Functional status was evaluated with the Functional Index Measure (FIM). A high functional capacity was defined above 64% of 18 items evaluating mental and motor capacity. Age, sex, type and localization of stroke, and kinetic rehabilitation were correlated with FIM using X a and logistic regression analysis. Results: There were 294 strokes patients followed for 3y (1157 hem- orrhagic and 137 ischenffc). Sixty six were lost during follow up and 111 (37.8%) were dead. Mean age of death was 71 y and mean age of alive patients was 63 y (p -- 0.11). F IM was performed in 117 patients (39.8%); 76.1% had high score for motor and 88% for Cognitive FIM. Seventy six percent had rehabilitation, a l though 44.9"/0 only had 15 days. The selected variables in logistic regression analysis associated with lffgh scores of motor F IM were age (i[3 - 0.054, p -- 0.008, 95"/0 IC -- -0.91 to -0.98), sex ( [3 -- - 1.53, p -- 0.003, 95% IC - -0 .07 to 0.60), and stroke type ([3 -- 1.54, 0.006, 95% IC 1.56 to 14.02); only age was assodated with High Cognitive FIM. Conclusion: Stroke mortality rate at three years was 37.8%. A higher functional motor status for alive patients was associated with women sex, age, and lacunar stroke.

1152 A prospective observational gtudy of ambulatory blood pressure monitoring and its prognostic value in Acute Stroke

Dong, Q1, Yang, Q1, Ding, H 1, Wang, L 1, Lu, j 1 ZDepartment Of Neurology, Huashan Hospital, Fudan University, Shanghai

Background: Elevated blood pressure is a common complication of acute stroke. Despite various studies, data on the prognostic sig- nificance of early blood pressure in stroke renmin unclear. Methods: We prospectively studied 91 patients (ischemic stroke, n - 53, intracerebral haemorrhage, n - 38) adnfftted in 48 hours of stroke onset. The early outcome was assessed 21 days after stroke onset. In ischernic stroke patients, poor outcome was defined as mRS _>3, in intracerebral haemorrhage patients, we use the mortality as dependent outcome. The late poor outcome was defined as mRS _>3 at 3 months. Result: The elevated blood pressure after stroke declined sponta- neously. For ischemic stroke a U-shaped relationship was observed between blood pressure and late outcome. The blood pressure for lowest frequency of poor outcome was 140 ~-' 160 m m H g (SBP) and 75 ~-' 80 m m H g ( D B P ) . However, only SBP _> 160 m m H g showed a significant relationship with poor outcome after adjusted other prognostic factors. For intracerebral haemorrhage the high MAP as well as NIHSS was prognostic factor for mortality at 21 days and poor outcome at 3 months. For every 1 m m H g above 85 mmHg, the risk of early mortality and late poor outcome increased by 21.2% and 27%. Conclusion: Blood pressure declined spontaneously after stroke. For ischernic stroke SBP _> 160 m m H g showed a significant relationship with early and late poor outcome. For intracerebral hemorrhage the lffgh M A P as well as NIHSS was prognostic factor for mortali ty at 21 days and poor outcome at 3 months.

1153 A gtudy on the Neuroprotective effects of rhEPO in Focal Cerebral Ischentia of nornml rats and acute DM rats

Dong, Q1, Lu, y1, Ren, H 1. ZDepartment of Neurology, Huashan Hospital, Fudan University, Shanghai

Background: Cerebral ischernia with Diabetes mellitus is more serious. Satisfactory results have been acquired from animal experiments and some clinical trials to treat cerebral ischernia with rhEPO. Method: We observed the influence of rhEPO on neurological deficits proport ion of infarcton volmne in focal cerebral ischenffc rats with acute D M and the expression o f EPOR. We employed acute STZ- induced D M rats. The animals underwent 1 hour of MCAO. 5000IU/ kg rhEPO was injected at the same time of reperfusion. Longa and NSS tests were performed to value neurological function. Farction parts were shown through crystal-violet staining and the expression of EPOR was observed by inununo -histochemfstry. Result: In D M rats, NSS of the rhEPO treated group at the 1st, 7th and 14th day after reperfusion were 10.63 ± 1.69, 9.50 ± 2.00 and 8.50 ± 1.69, the infarction volume proport ion (113.86 ± 3.38)% (110.96 ± 2.62)"/0 (7.24 ± 1.93)% respectively, significantly lower than those of the control group (P < 0.05). But the improvement of NSS and reduction of infarction volmne proportion in D M rats was of no difference from those in the normal ischemic ones (P > 0.05). Conehision: RhEPO can ameliorate neurological defidt and reduce infarction area of focal cerebral ischemic rats in both normal groups and acute D M ones. EPOR expression is up-regulated in the ischemic brain. Treatment of rhEPO can up-regulate its expression.

1154 VCAM-1 Mediated Neutrophil survival - a therapeutic target in Stroke

Douglas, M 1, Ross, E ~, Wong, S ~, Ross, E ~, Curnow, S ~, Scheel-Toelnner, D 2, Lord, j2, Salmon, M 2, Buckley, C 2. ZDivision of Neurosciences, University of Birmingham, United Kingdom; 2Division of Infection and Immunity, MRC Centre for Immune Regulation, Nstitute for Biomedical Research, University of Birmingham, United I~'ngdorn

Background: Stroke remains a major cause of death and disability and more potent therapies are required. Studies demonstrate that, following the initial vascular event, the extent of tissue destruction correlates with the size of the local neutrophilic infiltrate. Tiffs area of infarcted tissue expresses certain cell adhesion molecules (CAMs), particularly VCAM-I , that may modulate local neutrophil accumula- tion and resultant tissue damage. We therefore investigated the role of these CAMs on neutrophil apoptosis. Method: Neutrophils were isolated fi'om peripheral blood. Functional assays were carried out in the presence of recombinant CAMs. Spontaneous and Fas-induced apoptosis were quantified by FACS and cytospin morphological analysis. VCAM-I-79131 protein interactions were identified by immunopredpi ta t ion and western blotting. Depen- dence on PI3 kinase and NF-KB were demonstrated using the intffbitors LY294002 and SN50 respectively. Results: We have identified that VCAM-I inhibits neutrophil apop- tosis, an effect delivered through binding the integrin ~9131. The signaling pathway requires PI3 kinase, NF-~cB activation and de novo protein synthesis. Conclusion: Tiffs s tudy demonstrates a novel functional role for the neutrophil integrin ~9131 and suggests that the presence of VCAM-1 in infarcted brain tissue is likely to promote neutrophil accunmlation. Antagonizing this pathway could therefore form an attractive therapy for stroke.

1155 Clinical Protile of Stroke patients with associated risk factors and 30 day mortality rate