2008 D5 Program

Bet Your Life

on Research2008 District 5 MeetingApril 30-May 2, 2008

Hosted by Central Ohio Branch AALAS

Housing Information and Directions

Cherry Valley Lodge Room Rates

*Make hotel reservations directly with Cherry Valley Lodge*

Room Rates held until April 4 -

2299 Cherry Valley Road $129*/nightNewark, OH 43055 *If you stay for the weekend740.788.1200/800.788.8008 the same rate will apply.

th

From North Columbus

From Downtown Columbus

From Port Columbus International Airport

From Cleveland

From Zanesville

From Cincinnati

From Athens

Take SR 161 East to Granville. SR 161 turns into SR 37. Take SR 37 East to SR 16. Stay on SR 16.Turn right (South) at Cherry Valley Road stoplight. The Cherry Valley Lodge is located ¼ mile south ofSR 16 on the left side.

Take I-70 East to Granville/Lancaster SR 37 Exit. Follow SR 37 North towards Granville. Exit East on SR 16,continue 2 miles and turn right (South) at the Cherry Valley Road stoplight. The Cherry Valley Lodge islocated ¼ mile south of SR 16 on the left side.

From the International Gateway, take I-670 towards I-270. Exit I-270 North, following the I-270 outerbelt toRte. 161 East (New Albany) exit. Travel approximately 20 miles and 161 turns into SR 16. Continue travelingon SR 16 for approximately 3 miles and turn right (South) on to Cherry Valley Road at the stoplight. TheCherry Valley Lodge is located ¼ mile south of SR 16 on the left side.

Take I-71 south to SR 13 south. Take SR 13 south approximately 50 miles to SR 16 west on Newark. Take SR16 west approximately 4 miles to the Cherry Valley Road stoplight and turn left. The Cherry Valley Lodge islocated ¼ mile south of SR 16 on the left side.

Take I-70 West to Granville/Lancaster SR 37 Exit. Follow SR 37 North towards Granville. Exit East on SR 16.Continue 2 miles and turn right (South) at the Cherry Valley Road stoplight. The Cherry Valley Lodge islocated ¼ mile south of SR 16 on the left side.

Take I-71 North to I-270 North (East) to I-70 East. Take I-70 to Granville/Lancaster SR 37 Exit. Follow SR 37North towards Granville. Exit East on SR 16. Continue 2 miles and turn right (South) at the Cherry ValleyRoad stoplight. The Cherry Valley Lodge is located ¼ mile south of SR 16 on the left side.

Take SR 33 West to Lancaster and turn right (North) at the Main St. stoplight; go 1 block and turn left (West) atSR 37 stoplight. Follow SR 37 North towards Granville. Exit East on SR 16, continue 2 miles and turn right(South) at the Cherry Valley Road stoplight. The Cherry Valley Lodge is located ¼ mile south of SR 16 on theleft side.

♠ No Shuttle ProvidedCherry Valley Lodge located approximately 25 miles

From Port Columbus Airport

♠

www.cherryvalleylodge.com

Book Now!

Page 24 District 5 - 2008 COBAALAS

District 5 - 2008 COBAALAS Page 3Page 22 District 5 - 2008 COBAALAS

District 5 AALAS 2008 Annual Meeting“Bet Your Life on Research”

April 30 – May 2, 2008Cherry Valley Lodge

Newark, OH

Presenter Registration Form

First Name Last Name Credentials/Degrees:

Institution

Mailing Address

City State Zip

Telephone Fax Email

Presentation Title

Audio Visual Requirements (Check All Required): Slide Projector PowerPoint Projector & PCOverhead Projector Other

Registration Fees (Final Payment due April 4th)Cancellation/Refund Policy: D5 registration fees are refundable through 4-16-08 minus a $25 processing fee!

Options *Fee* Total

Registration WaivedWelcome Reception April 30th WaivedMealsIndividual Lunch: offered only Thursday $20Individual Dinner: (check one) Wednesday, Thursday, both $40WorkshopsBASi “Culex Automated DMPK in Vivo Sampling”(check one) Thursday 8:45am-9:45am 10:15am-11:15am

$10

“A Program for Simulated Rodent Surgical Training”Friday morning

$10

ToursSterigenics Facility Tour – limited to the first 25 registrants $10Poker Run: See additional form – register onsite feeGolf Outing: has a separate form – mail to Dale Ettel Total

� Two reduced fee registrations are available as scholarships for individuals that may need financial assistance inorder to attend. For details contact LaTonya Mitchell: [email protected]. Apply by March 14th, 2008.

Do Not Send Cash:Make checks or money orders payable to: COBAALAS

Mail completed form with payment to: Jennifer Quick-Kerns, Battelle Memorial Institute, 505 King Ave., JM-7-110,Columbus, OH 43201. Email: [email protected]. Phone: 614.424.5468. Fa•: 614.458.5468.

♠ ♠

♠ ♠

Wednesday April 30Golf Outing

Registration

Exhibit Set-Up

Poster Set-Up

Sterigenics Tour

Poker Run

Welcome Party “Casino Night”

Registration

Continental Breakfast

Exhibit Set-Up

Poster Set-Up

9:30 AMRaccoon International Golf CourseGranville, Ohio

12:00 PM - 5:30 PMCherry Valley Lodge Lobby

12:00 PM - 5:30 PMJubilee Rms A-E

12:00 PM - 5:00 PMFoyer outside Blossom Rms I-IV

1:30 PMSterigenicsWesterville, Ohio

1:30 PMCherry Valley Lodge Lobby

6:00 PM -10:30 PMBlossom Rms III and IV

7:30 AM - 5:00 PMCherry Valley Lodge Lobby

7:30 AM 9:00 AMFoyer outside Jubilee Rms A-E

7:30 AM - 8:00 AMJubilee Rms A-E

7:30 AM 8:00 AMFoyer outside Blossom Rms I-IV

Thursday May 1

First Timers Breakfast7:30 AM 8:30 AMBlossom Rm IV

♠ ♠Friday May 2Registration


District 5 Council Breakfast

TBR Breakfast

Sessions and Workshops

7:30 AM 9:00 AMCherry Valley Lodge Lobby


7:30 AM 9:00 AMCherry Valley Lodge


9:00 AM 11:00 AMBlossom Rms I-IV

♠ ♠Thursday May 1(Continued)

Poster Session Open

Silent Auction

Opening Remarks


Box Lunch


Poster Authors Present/Poster Judging

Cocktail Hour

Awards Banquet and Social

8:00 AM 5:00 PMFoyer outside Blossom Rms I-IV

8:00 AM - 5:00 PMFoyer outside Jubilee Rms A-E

8:30 AM- 8:45 AMBlossom Rm IV


11:30 AM -1:00 PMCherry Valley Lodge

1:15 PM 4:00 PMBlossom Rms I-IV

4:00 PM 5:00 PMFoyer outside Blossom Rms I-IV

6:00 PM- 7:00 PMCherry Valley Lodg

7:00 PM 9:00 PMJubilee Rms A-E

e

Exhibits Open8:00 AM 5:00 PMJubilee Rms A-E

♠See You

Next Year!2009 District 5 Meeting

The Galt House Louisville, KY


Preliminary Schedule of Events

TECHNICIAN FUN FAIR

Join us for

in the vendor area!fun and excitement

Test your knowledge of vendors and their products, AALAS,and the 2008 District 5 Program

Talk with vendors and committee members to find their answers. You can also use the meetingprogram.

Correct answers make you eligible for a drawing held at the Thursday night banguet.

All participants will receive a certificate suitable for framing


Preliminary Meeting Presentation Schedule We Are Betting

You Can Be The Winner!

The District 5 2008 AALAS meeting planning committee would likeyou to participate in a silent auction.

The auction closes at 5:00 PM Thursday and the highestbidders will be announced at the banquet Thursday night.

If you wish to donate items please contact:

At Our

During the

Individuals, vendors, and local branches are invited to donate itemsto be auctioned.

Jennifer Quick-Kerns, Battelle Memorial Institute, 505 King Ave., JM-7-110,Columbus, OH 43201. Email: . Phone: 614.424.5468

Silent Auction

[email protected]

District 5 AALAS MeetingApril 30 -May 2 , 2008

Newark, Ohio

th nd

♠

♠

♠

8:30 AM – 8:45 AM Opening Remarks

Scientific Technical Special Topics Workshops

8:45 AM – 9:30 AMBill Winsley

‘Controlled Substances:Licensing, Use and

Storage’

Karen HallbergCoyote Vocalization 8:45 AM – 9:45 AM

BASi Workshop 1

(Limited to 20participants)

9:45 AM – 10:30 AMDr. Gehrt

‘Visceral LarvalMigrans’

Tech Tips:-Mark Czarneski &

Paul Lorcheim-Peggy Nahorski

-Dr. Laurie Goodchild& Kelly Brown-Chris Lyons

Christopher J. WinslowStone Labs

‘Aquatic Research’

10:45 AM – 11:30 AM

Dr. Noé Tirado-Muñiz‘Public Health Issues in

Laboratory AnimalPopulations’

Pam Snider‘Careers for Veterinary

Technicians’

Mike Seidel‘Playing Possum with

the Ohio WildlifeCenter’

10:15 AM – 11:15 AMBASi Workshop 2


11:30 AM - 1:00 PM LUNCH

1:15 PM – 2:00 PM

D’Anna Jensen‘Alternative Searchesand Resources AWIChas for the Research

Community’

Dr. Wynn‘Rattlesnake Husbandry’

2:15 PM – 3:00 PM

Dr. Tracy Peace‘Holding and Playing

the Right Cards Duringan AAALAC Site Visit’

Dr. Tony Buffingtonand Judi Stella‘Environmental

Enrichment for Cats:Lessons Learned from

a Stress-sensitivePopulation ’

3:15 PM – 4:00 PM

Dr. Joe Simmons‘Update for Murine

Noroviruses andPrimate Retroviruses’

Dr. Jori Leszczynski‘Increased OperatingEfficiencies Translateinto Decreased Utility

and Labor Costs’

Tech Tips:-Jennifer Quick-Kerns-Dr. Xin-an Pu-Ming Lu

Friday, May 2

9:00 AM – 9:45 AM

Dr. ChristianNewcomer

‘Animal Models ofTuberculosis’

Dr. Oscar A. BermeoBlanco

‘Improvements to theDog Bile Cannulation

Surgery Model’

Jennifer Brandt‘Coping Mechanisms forPet Loss/Bereavement’

9:50 AM – 10:45 AM

Robin Trundy'Animal BiosafetyLevels Explained:

Something forEveryone'

John Merrill‘Ferret Husbandry,

Enrichment,Anesthesia and

Intratracheal Dosing’

9:00 am – 11:00 am

A Program for SimulatedRodent Surgical Training


2008 D5 GOLF REGISTRATION: General Membership

District 5 AALAS Annual Meeting, Newark, OH., APRIL 30, 2008.

Golf Outing Sponsors: Anderson Bed-O’Cobs, Lab Products, and

Purina Mills LabDiet.

Where: Raccoon International Golf Course, 3275 Worthington

Road S.W., Granville, OH 43023; ph: 740-587-0921

www.raccooninternational.com

Date: Wednesday, APRIL 30, 2008… starting tee time 9:00 AM

Game: 4 person/ best ball scramble format with Lunch &

Your Favorite Beverage. Prizes……….

Please join us for a great tradition of fun & camaraderie!

………………………………………………………………………

General Membership Registration Form:… Non-Vendors Only

Name_________________________________________________

Company______________________________________________

Address________________________________________________

Work phone___________________email_____________________

Short description of your golf

score___________________________________________________

…………………………………………………………………………

Please Return your golf reservation by mail, fax, email, before

APRIL 15th

if at all possible. Or, give me a call anytime to reserve

a spot. Thanks…..

TO:Dale Ettel, Purina Mills, 1002 Woodmount Drive, New Albany, IN 47150

Office: 812-949-6070; fax: 812-949-7073; mobile: 812-968-0736

Email: [email protected]

……………………………………………………………………………………

Look forward to seeing you at the Raccoon International Golf Course.

Workshops:In Vivo

Speakers:

Culex Automated DMPK Sampling

A Program for Simulated Rodent Surgical Training

Controlled Substances: Licensing, Use and Storage

Holding and Playing the Right Cards During an AAALAC Site Visit

Natasha Nikolaidis and Robyn GuinnBASi

Carol StevensCentral Michigan University

Bill WinsleyExecutive Director, Ohio State Board of Pharmacy

Tracy A. Peace, DVM, MS, DACLAMBattelle Memorial Institute

The Culex Automated Sampling System facilitates DM / PK / PD studies. Via the Culex, automation is used to reduce, refine, and replace thenumbers of animals needed to run traditional pharmacokinetic, drug metabolism and pharmacodynamic studies. BASi's Culex system can automatecollection of blood, bile, urine, feces, dialysates, and more all from awake and freely moving animals.

The purpose of the Culex workshop is to demonstrate and discuss how automated DMPK sampling benefits the animals, the animal technician, andthe research process. The workshop will provide a live animal demonstration (rats and mice) of the Culex in operation. The didactic session willdiscuss the flexible uses for Culex.

The Association for Assessment and Accreditation of Laboratory Animal Care, International (AAALAC) performs site visits of accredited facilitiesevery three years. These site visits can be a stressful event for any facility (conjuring up images of moaning and gnashing of teeth)! Topics to bediscussed include my suggestions relative to preparation for a site visit including readiness tips for animal technicians and the IACUC. Conduct ofthe site visit itself and the importance of the post-site visit communication will also be discussed. Being well prepared is your “ace in the hole”!

In Vivo

For the inexperienced individual, learning surgical techniques can be very taxing. The use of simulation models provides a non-taxing learning experienceand incorporates the The rodent surgery dry lab training program was developed to assist educational andresearch institutions in providing proficient non-stressful training in performing basic surgical techniques using handmade, cost-effective simulationmodels. The rodent surgery dry lab includes modules pertaining to: animal preparation and monitoring; surgical hand scrubbing and gloving; instrumenthandling and maintaining an aseptic surgical field; and suturing techniques. Observations and course evaluation feedback forms and follow upperformance monitoring indicate that this dry lab maximizes successful techniques on the live animal, illustrates problems that may occur as well ascorrection of errors in technique, and allows for repeated practice while building trainee self-confidence.

This training module enhances the learning process by providing the trainee with a non-taxing, hands-on learning experience, in contrast to other moreconventional training methods. We observed that students completing the lab have developed essential skills that enable them to be more confident,comfortable, and focused on the basic surgery techniques at hand. Furthermore, this training lab builds an opportunity for gentleness and genuineconcern for the animals in research and teaching. This training module has proven to be highly successful in training individuals in the research andeducational fields.

“3 R s” (Reduce, Replace, and Refine).

Presentation Abstracts

Page 18 District 5 - 2008 COBAALAS District 5 - 2008 COBAALAS Page 7

Public Health Issues in Laboratory Animal Populations

Increased Operating Efficiencies Translate into Decreased Utility and Labor Costs

Improvements to the Dog Bile Cannulation Surgery Model

Murine Norovirus in Laboratory Mice

Noé Tirado-Muñiz, DMV, DVM, MSBattelle Memorial Institute

Jori Leszczynski, DVMNational Jewish Medical and Research Center

Oscar A. Bermeo Blanco, DVM, RLATGBattelle Memorial Institute

Joe H. Simmons, DVM, PhD, DACLAMCharles River Laboratories

Although not as common as in the past, zoonotic diseases (diseases transmissible from animals to humans) still play an important role in our dailyactivities. Whether you are working with wild-caught animals, immune- compromised animal models, animal models of infectious diseases or othersettings, the knowledge of zoonotic disease control and prevention is of paramount importance for your safety and the safety of coworkers. We willreview simple but key aspects of zoonotic disease transmission prevention as well as a few cases of public health interest.

There are many ways that an animal facility can decrease costs to the institution. Over the past year, National Jewish has undertaken a cage washremodeling plan that included aggressive evaluation of water use. Eventually, equipment purchases were partially determined by their operatingefficiency. This has translated into thousands of dollars in initial incentives from the Denver Water Department and thousands of dollars each year inutility savings. In addition, National Jewish has also undertaken personnel initiatives such as flex-time and multi-faceted job responsibilities thathave saved money on overtime and turnover, as well as anectodatally increased employee satisfaction.

Successful canine bile cannulation surgery is critical to the performance of pharmacodynamic studies in preclinical toxicology. This presentation willdemonstrate improvements which enhanced the performance and longevity of the dog bile cannulated model. The lessons learned from previousmodels helped to refine bile collection techniques with minimal physiological effects to the animal. Topics to be discussed include acclimation to feed,collars and jackets prior to surgery, the surgical procedure itself and post-operative care. Don't gamble on the success of a surgicalmodelpreparation is the key!

Murine norovirus (MNV) is a newly emerging mouse that was first described by Stephanie Karst and colleagues in 2003. They reportedthat mice with severe deficiencies in their innate immune systems, specifically , died sporadically after infection by MNV-1. This sparked agreat deal of interest in MNV, since norovirus infections are reported to be responsible for 90% of human nonbacterial gastroenteritis, worldwide, andthere are no small animal models of human norovirus infection.

Noroviruses belong to a large and diverse group of nonenveloped RNA viruses that, in addition to humans, infect a variety of animals including cats,rabbits, pigs, sea lions and cattle. MNV has created both interest and consternation within the laboratory animal community because its prevalencein contemporary mouse colonies, as assessed by published reports from research animal diagnostic laboratories, ranges from 22-29% of the micetested: indicating that MNV is by far the most prevalent virus in laboratory mouse colonies. To date, MNV field strains have not been associated withsigns of clinical disease in immunocompetent mice; however, MNV-1 does infect macrophages, a key component of the immune system.

In this talk we will discuss the pathobiology of MNV infections in laboratory mice, include the biology of the and the discovery,pathogenesis, diversity and biology of MNV. Current methods for molecular and serologic detection of MNV will be covered and the potential impactof MNV on biomedical research will be discussed.

Calicivirus

STAT-1

Calicivirdae

Presentation Abstracts (continued)

Veggie Tails: A Novel Training Method

Strategies for the Elimination of Mouse Parvovirus

Adoption as an Alternative Destiny for Retiring Research Dogs

Christina Zehender; Joshua HittleCharles River Laboratories Preclinical Services Ohio

Marie Koenig, Gerald SmithEli Lilly and Co.

Andrew S. Fix , Jennifer L. Borger , Robin Cooper , Gwyn L. Jeans ,Becky R. Rupert , Mark A. Tetrick , Jennifer L. WheatleyP&G Central Product Safety P&G Pet Care

The lateral tail vein is a common method of administering test material in rat models, primarily in toxicology research. Learning and becomingproficient at the procedure can be difficult for technicians and place stress on practice animals. We propose using an alternative method of learningthe procedure on a non-animal model. Celery offers a great alternative to rat models due to the vascular tissue running along the stalk of the plant.Injection of methylene blue into the vascular tissue of celery allows a technician to properly align the syringe and place the needle at the properdepth into the plant tissue as per proper tail IV functions. The injection of methylene blue also allows the technician to visually confirm properplacement of the syringe, as the compound will travel through the celery vascular tissue. These new procedures with reduce the amount of practiceanimals in the laboratory and reduce the amount of stress on the animals.

Eliminating mouse parvovirus (MPV) from our continuously occupied multi-user facility has been difficult. Sentinels (Hsd: ICR, CD-1 mice) weregroup-housed in non-filtered polycarbonate cages on room floors, exposed to soiled bedding weekly and sampled every 6 wk. Detection sensitivityby the sentinels was likely compromised by a low prevalence of infection in resident mice and poor transmission to sentinels. From 2001 to 2005, oursentinel program indicated 30% of our rooms had a history of MPV-positive sentinels. The incidence of MPV was essentially unchanged during theperiod of time, in spite of depopulation and decontamination of MPV-positive rooms. Specific epidemiological traits were observed in these rooms;the eradication strategy was modified to focus on rooms with these traits. MPV-positive rooms usually had one of the following characteristics:breeding colonies, multiple users, or rooms where mice were frequently transferred to or from testing laboratories outside the vivarium. Therefore,the strategy was modified to periodically depopulate and decontaminate rooms with these characteristics, regardless of negative sentinel results.Advance notice was given to researchers to stop receiving animals into a room scheduled for decontamination and to complete their studies in thatroom. The schedule was flexible to avoid interfering with research projects. Since MPV persistence is also affected by its resistance to environmentalinactivation, decontamination procedures were modified from liquid chemicals only to an additional method for decontaminating rooms, laboratoriesand equipment using vaporized hydrogen peroxide (VHP). VHP is a process that maintains vapor concentration below the condensation point toprotect sensitive instrumentation. The number of breeding rooms was also decreased. Subsequently, MPV infections were dramatically reduced.Since 2005, 18 mouse rooms and 18 laboratory/procedure rooms were depopulated and VHP-decontaminated. Incidence of MPV-positive rooms hasdecreased to about 14% since 2005, and sentinels have been MPV-negative for 9 mo.

Adoption is a viable destiny alternative for animals involved in some, but not all, types of research service. We describe an adoption program fordogs implemented at The Procter and Gamble Company. This program included: 1) a set of principles for adoption, with inclusion/exclusion criteria,2) an adoptability classification system based on thorough medical and behavioral assessments, 3) a specific acclimation and training plan for eachdog, and 4) a screening and approval process for prospective owners. Adoption candidates were dogs from a wide age range and service history.Dogs received complete pre-adoption assessments and were grouped according to the adoptability classification system. This helped identify areasto address during pre-adoption acclimation and training. Specific training or other interventions were provided based on individual needs, includingleash training and exposure to environments likely to be encountered post-adoption. A number of dogs were moved through this program andsuccessfully placed in homes. Implementation of this program required the collaborative efforts of staff from the veterinary, animal welfare, andfacility operation areas. As a way to improve the quality of life of retired research dogs and decrease the number of animals euthanized in non-terminal research, we advocate broader consideration of active intervention programs and adoption as an alternate destiny for these animals.

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Poster Abstracts (continued)


Macaque Retrovirus Infections: Potential Confounding Variables in Biomedical Research

Playing Possum with the Ohio Wildlife Center

Animal Biosafety Levels Explained: Something for Everyone

Ferret Husbandry, Enrichment, Anesthesia and Intratracheal Dosing

Animal Models of Tuberculosis

Coping Mechanisms for Pet Loss/Bereavement

Veterinary Technicians: Career Options (Private Practice, Research)

Alternative Searches and Resources AWIC has for the Research Community


Mike SeidelOhio Wildlife Center

Robin Trundy, MS, CBSPBattelle Biomedical Research Center

John W. Merrill, BS, LATGBattelle Memorial Institute

Christian E. Newcomer, VMDJohns Hopkins

Jennifer Brandt, MSW, LISW, PhDThe Ohio State University

Pamela SniderColumbus State Community College

D'Anna JensenTechnical Information SpecialistAnimal Welfare Information Center (AWIC)

It is well recognized that adventitious infectious agents can interfere with biomedical research by increasing morbidity, mortality, and variability inscientific data, thus confounding research. Some infectious agents are acute and self-limiting, while others cause persistent or latent life-longinfections. Among the group of agents that cause life-long infections are the retroviruses. Retroviruses are a large and diverse group of enveloped,single stranded RNA viruses that are unique among viruses they possess a complement of enzymes, including reverse transcriptase, that allowsthem to reverse transcribe their RNA genomes into DNA and then insert that DNA into the host's chromosomal DNA. Once the retrovirus isinserted into the host's genome it is called a DNA provirus. The integrated DNA provirus allows retroviruses to persistently infect their host and toavoid surveillance by the host's immune system. Retroviruses can infect a wide variety of cells within their host, often including cells of the immunesystem. Retrovirus infections can be subtle and subclinical, or they can result in debilitating immunosuppression which makes animals susceptibleto a wide variety of opportunistic infectious agents and cancers.There are currently four well described retroviruses known to infect laboratory macaques: simian immunodeficiency virus (SIV), simian type Dretroviruses 1-5 (SRV), simian T-lymphotropic virus (STLV) and simian foamy virus (SFV). In this presentation we will discuss the pathogenesis ifeach of these agents including diagnosis, and potential to impact biomedical research and colony health.

Presenting Ohio's native wildlife with characteristics of habitat, enrichment, etc. The presentation will field questions and discuss overall wildliferehabilitation and release here at the Ohio Wildlife Center.

A biosafety level is a combination of facilities, equipment, and practices designed to allow personnel to work with biohazardous material in amanner that is safe for them, and safe for the surrounding environment. When the biohazardous material is used in an animal model, there arespecial considerations that surpass issues that might arise in a typical lab setting. Depending on the nature of the biohazardous material (wild typeinfectious agents, agents that contain recombinant DNA molecules, etc.) and the species of the animal, different guidelines relative to biosafetylevels will apply. This presentation will review the definition of biosafety levels and describe the unique features of animal-related biosafety levelsunder different regulatory requirements.

Presentation Abstracts (continued)Poster Abstracts (continued)


Rabbit Group Housing (continued)

Reliable Old Caging Is NotAlways a Safe Bet

To this end at the University of Kentucky, Division of Laboratory Animal Resources (DLAR) we looked at group-housing as an alternative to individualcaging. Our goal was simple, to improve the quality of care and provide a group setting for short and long term rabbits, while still being able to isolatethe individual. Group housing rabbits provides increased space to allow them freedom of movement to carry out normal activities such as hopping,stretching out, sitting up with erect ears, rearing and leaping. Rabbits are naturally gregarious and group-housing allows them to have socialinteractions such as grooming, playing, and lying together. It also offers greater opportunity for environmental enrichment such as foraging and theability to have multiple levels within the environment. Another benefit of group/pen housing was increased activity to help counteract the forcedsedate life style of long term animals by providing opportunities to exercise. This increases appetite, decreases obesity, helps prevent conditionssuch as “sore hocks”, hairballs, and diarrhea) and adds to overall general health.

An intact male dog was presented with three approximately 1cm lesions of unknown origin on the anterior aspect of its scrotum. The lesionsappeared somewhat raised and moist but were not obviously infected. Upon further observation, the dog was viewed jumping up at the front gate ofits run causing the scrotum to come into momentary contact with an obscure recessed area 19 inches above the floor on the gate of the run. Theseruns are in compliance with USDA regulations and have been used uneventfully for over 20 years.

The obscure recessed area is a groove designed to shelter the ends of the vertical bars in gate from contact with the dog and is in direct line with thespecific area of the dog's scrotum when he was standing on his hind limbs. The lesions were the result of low grade trauma due to contact from thisarea of the gate. To prevent further trauma the groove was filled with silicone caulk so that the ends of the bars were covered. The lesions wereinitially treated with a 10% betadine solution and silver sulfadiazine ointment. Following initial treatment and the application of silicon caulk, thelesions resolved uneventfully.

While this type of lesion had never been observed on the hundreds of dogs housed in these runs over 20 years, it demonstrates that there are alwaysodds that reliable old caging is not always a good bet.

Canine Home Environment Acclimation Program (A Conditioning Program to Better Prepare Research Dogs for Adoption)

Temperature and Humidity Alterations Due to Caging Type

Jennifer L. Wheatley, Gwyn L. Jeans, Kevin D. Corcoran, Andrew S. FixProctor and Gamble

Lindsay Zinser, Greg BoivinCincinnati VA Medical Center

Many canines retiring from research service are deemed eligible for adoption. In an effort to better prepare these dogs for a successful transition to ahome environment, we describe an acclimation program implemented at The Procter and Gamble Company. This program includes a specificacclimation and training plan for each dog with exposure to environments and stimuli likely to be encountered post-adoption. Our goal is to addressany existing behavioral or socialization issues, introduce housebreaking principals, and familiarize dogs with common household items prior toadoption. Acclimation within an artificial home environment and other interventions are provided based on individual animal needs. We have foundthis program to be beneficial and enriching not only for the canine subjects, but also for the animal care and veterinary staff members who haveparticipated. The program has been utilized for many dogs which are now successfully placed in homes. As a way to improve the transition to ahome environment for canine adoption candidates, while increasing job satisfaction for employees, we advocate consideration of an acclimationprogram such as this.

The Guide for the Care and Use of Laboratory Animals states “The relative humidity should generally be maintained within a range of 30-70% throughoutthe year, and the recommended dry bulb temperature for mice and rats is between 64 and 79° F.” With the advent of new caging types, standard monitoringof room data may be insufficient to truly evaluate the local rodent environmental conditions. In this study we examined the effect of different housingconditions for mice and rats and compared temperature and humidity with room values. Mice housed in PIV cages had on average an increase in cagetemperature of 7.2°F and a 4.5% decrease in humidity. Both values were significant (p<0.0001). Mice and rats in conventional shoebox cages withmicrofilter tops had significant increases (p<0.001) in both temperature and humidity (mice 3.7°F & 6.3%; rats 3.1°F & 3.4% respectively). The number ofanimals in the cage (all below Guide space requirements), significantly alter the changes identified. Increasing biomass in the cage increased thetemperature and humidity proportionally. Despite increased air flow, the PIV racks consistently had higher temperatures than static isolator cages. Werecommend that these changes in temperature and humidity be considered when establishing room parameters. In addition, extreme care must beobserved when room temperatures increase due to HVAC failure because the in cage temperatures are significantly higher than the room temperature.

Candace HedrickThe Ohio State University, University Laboratory Animal Resources

Environmental Enrichment for Cats Lessons Learned from a Stress-sensitive Population

Coyote Vocalization

Visceral Larval Migrans

Dr. James Buffington and Judi StellaThe Ohio State University

Karen HallbergThe Ohio State University

Dr. GehrtThe Ohio State University

Dr. WynnThe Ohio State UniversityRattlesnake Husbandry

Mr. WinslowStone Laboratory

Our laboratory studies “FUS” in cats. Previously known as “feline urologic syndrome” the term is now known to describe a “feeling unexplainedsyndrome”. Our studies have found that these cats appear to have a developmental disorder that leads to a sensitized stress response system.Physiological consequences of this sensitization result in abnormalities of a variety of body systems, and the destruction of millions of cats in theUS each Year. It also is a naturally occurring disease analog of an idiopathic pain syndrome in humans called interstitial cystitis. Although usuallynot lethal (notwithstanding regular reports of suicide among sufferers), the disease can lead to a lifetime of suffering.

During the past few years we have documented the stress sensitivity of these cats, and found that we can resolve clinical signs (but not thedisease) by environmental enrichment (EE). Based on these studies, we have improved clinical approaches for therapy of client-owned cats.( )

During this presentation we will present our findings related to neural, endocrine and immune abnormalities in these cats, our approaches to EE,and discuss some of the mechanisms that may underlie the effects of EE in cats, as well as in other species. We also will present data supportingthe idea that good husbandry, EE and low stress environments benefit the animals, the researchers and the animal care staff. These benefitsinclude increased ease of handling, cage hygiene, and reduced sickness behaviors.Questions from and discussion are welcome so we all can learn from our interaction.

Karen Hallberg is currently a PhD Candidate in the Department of Evolution, Ecology, & Organismal Biology (EEOB) at The Ohio State University.Her dissertation research investigates the role of chorus howling in coyote social and territorial strategies.

Karen completed her MA in Psychology, in the area of psychobiology, at Ohio State in 2000 and transferred to the Department of EEOB in 2001.Having studied captive primates in her bachelor's and master's programs, Karen sought to explore the behavior of animals in their natural habitatfor her PhD.

Although her graduate research has focused on questions of animal behavior and bioacoustics, her work with wild coyote populations has ledKaren to develop a keen interest in issues of human-wildlife conflict and the potential of public education to promote coexistence.

Www.indoorcat.org


DON’T FORGET!

Poker RunApril 30

1:30 p.m.$5 ♠



Evaluation of Non-human Primate Environmental Enrichment Program

Rat Neonate Oral Gavage and Intravenous Dosing Procedures

Catheterization of the Medial Artery in Rabbits

Colors of the StripUsing Color for Monthly Room Logs

Douglas J. ChieffeCleveland Clinic

Julie Roller and Linda UnrueCharles River Laboratories Preclinical Services Ohio

Julie Roller and Erica ConawayCharles River Laboratories Preclinical Services Ohio

Kathy Hopwood and Shannon WellsThe Ohio State UniversityUniversity Laboratory Animal Resources

Wild primates live in very complex social environments and are constantly looking for food, water, and mates. These wild primates are stimulated allday. In the laboratory setting there needs to be complete control over the subjects of a study, making it impossible to recreate the naturalenvironment of a primate. Studies have shown that under-stimulated primates may suffer from stress which can cause physiological problems. Usingprimates with physiological problems in research studies as a model of normal function is not a reliable source of information.

The Animal Welfare Act requires that laboratory animals be provided with environmental enrichment to promote psychological well-being.Environmental enrichment includes novel foods and toys that give the primates stimulation. The longer a monkey manipulates the enrichment, thebetter because it is stimulating the monkey for longer. The Cleveland Clinic Foundation provides its monkeys with many novel foods and toys.

The idea for this poster comes from projects that were worked on at Charles River Laboratories, Ohio Division. The technicians involved had todevelop a method and procedure for oral gavage and bolus intravenous dosing on rat pups ranging in age from 7 10 days old. The poster is goingto outline the materials utilized to accomplish this very difficult dosing technique. An illustration of each technique is also going to be supplied, as wellas some helpful hints for successfully accomplishing each route of dosing.

The idea for this poster comes from projects that were worked on at Charles River Laboratories, Ohio Division. The technicians involved had todevelop a method and procedure for inserting and maintaining a catheter in the medial artery of a New Zealand White rabbit. The poster will illustratethe placement of the catheter, as well as the materials utilized. The procedure for placing the catheter will be explained, as well as the procedurefollowed to maintain the patency (we have kept catheters patent for up to 12 hours). A sample blood collection regimen will be included with helpfulhints for scheduling and collection of samples. Safe removal of the catheter will be discussed, as well as some pointers for a successfulcatheterization.

Often in monthly room logs the days and weeks seem to blend together. It is usually hard to keep track of where a week ends and another begins.Using color coding, we have simplified the monthly room logs. We are using different colors for each set of weekly requirementsthere are tasks thatneed to be done three times per week, two times per week, and every two weeks. It is easier for the husbandry employees and supervisors to seewhich tasks need to be completed, which have already been completed, and what has not yet been completed each week. The poster will displaythe original form, the new colored form and the advantages for the new system.

Rabbit Group HousingEboni Lewis, B.S. RALAT, JoeAnn Croxford, A.A. RLATG, Margaret A. Hankes-Pena, RLATGUniversity of Kentucky

Animal facilities generally house their rabbits in standard caging. The size of the caging, as outlined in The Guide For the Humane Care and Use ofLaboratory Animals specifies the minimum square footage allotted to individually housed rabbits both in area and height, but does not take intoaccount the natural behavioral traits of the animal. It does address, however, environmental enrichment and states that it is desirable for socialanimals such as rabbits to be group housed when possible. (Continued on next page.)

Selecting the Right Decontaminating Agent for Rooms

What is Clean Anyway? IVC Cleaning Practices

Is it Time to Replace Your Newsletter with a Blog?

Blood Collection from the Lateral Metatarsal Vein in the Guinea Pig

Mark Czarneski / Paul LorcheimClorDiSys Solutions Inc.

Peggy NahorskiAlternative Design Mfg

Chris LyonsAssociate Executive Director, AALAS

Dr. Laurie Goodchild and Kelly Brown, BS, LATG, VTThe Research Institute at Nationwide Children's Hospital

This presentation describes and compares the primary methods of sterilizing / decontaminating clean rooms. The methods considered includegaseous systems, vapor systems, misting systems, and fogging, as well as manual spray and wipe techniques. The goal is to give the listeners anunderstanding of what the benefits and drawbacks of each method are so that an educated decision can be made as to what methods are bestsuited for different required levels of decontamination. It describes the penetrating ability of gaseous methods, the simplicity of spray and wipetechniques, the cost effectiveness of fogging and formaldehyde gassing, and issues of “wet” vapors versus “dry” vapors.The practical aspects of the different methods are also compared and contrasted. Room size and configuration limitations are discussed. Thepractical aspects of empty rooms versus “cluttered” rooms are also considered.This presentation is unique in that in compares all major methods, systems, and techniques in their effectiveness, ease of use, relative cost, andpractical application. The significance of this is that by comparing the various methods, the proper method can be utilized for the desired level ofdecontamination that the user wants and requires for each particular application.

Presentation reviews questions vivarium managers need to ask when developing proper cleaning and sanitizing processes for vent racks (IVCs).How important is it to clean inside a vent rack and how easy is it to do, will be some of the questions addressed. The presenter will then illustratewhat some vent rack manufacturers are doing to make cleaning IVCs easier and more efficient with facility washing equipment.

The challenges of publishing a Branch newsletter include finding and teaching someone to lay out articles, dealing with printer and mailinghassles, updating mailing addresses all the time, and maintaining funds for printing and mailing. Well maybe now's the time to consider replacingthe newsletter with a blog. The blog platform continues to evolve and is ideal for resource sites and newsletters. In this presentation, discover howa blog can save you money, keep your members informed, save you time, and generate advertising revenue for your Branch.

The Animal Rule

In-vitro Fertilization for Mouse Colony Management

Assisting Transgenic Production - Maximize Mouse Embryo Yield by Superovulation

Jennifer Quick Kerns, AS, RVT, RLATG, RQAP-GLPBattelle Biomedical Research Center

Xin-an Pu, PhD

The Ohio State University

Ming LuThe Ohio State University

New to the lab animal world and the FDA is a new set of regulations by the FDA known as the "Animal Rule". 21 CFR Parts 314 and 601 arenewer regulations that are still under development. To be used in combination with 21 CFR Part 58, the Good Laboratory Practices, this set ofregulations has been developed to provide evidence needed to demonstrate effectiveness of new drugs when human efficacy studies are notethical or feasible. The FDA may approve a product for which human safety has been established, and the results of adequate and well-controlledanimal studies establish that the product is reasonably likely to provide clinical benefit in humans. This can only be used if human clinical efficacytrials are not feasible or are not ethical. This set of new regulations is sure to change the laboratory animal world forever.

Tech TipsPoster Abstracts (continued)

Comparison of Feline Reproduction Success: Female Presented in Male's Home Environment vs. Male Presented in Female'sHome Environment

The Positive Impact of P&G Pet Care's Early Feline Socialization Program

Leann King, Deron SmithP & G Pet Care

Kristina Grider, Deron SmithP & G Pet Care

The P&G Pet Care Pet Health and Nutrition Center (PHNC) Cat Colony implemented a breeding program to maintain our specific pathogen-freestatus. In the PHNC environment, it would be advantageous to bring the male to the female's home for mating due to our housing requirements.This is not the traditional method based on the concern of how the male will perform in a new environment. In feral cat populations, the tom'sterritory will overlap the queen's territory. The tom will essentially travel into the queen's home territory. The belief is that taking the male to thefemale would allow the program to feel more natural for the animals and have equivalent or better reproduction results. The poster will outline themating processes used and the findings.

The P&G Pet Care Pet Health & Nutrition Center Cat Colony breeding program was designed to produce healthy kittens that are well socialized,easily adapt to change in the research environment, and are readily adoptable.

The key components of the program that will be presented include the following:

Kitten-to-Human and conspecific socialization.Kitten training using positive reinforcement and desensitization techniquesKitten acclimation methodology for procedures such as healthcare restraint techniquesIntroduction to environmental stimuli (i.e. Olfactory scents, sounds, textures, diets)

The positive impact of the Early Socialization Program will also be subjectively presented in the poster. Also, plans for objective measures will beoffered.

Innovative Venipuncture Method for the Feline SpeciesCindy LanmanP & G Pet Care

In every capacity of research and in every facility, it can be assumed that methodology refinements come to the minds of technicians quite often. Inthe case of feline restraint for blood collections, new ways to reduce the stressful nature of these procedures for both the felines and the technicianshave been difficult to uncover. This innovative venipuncture method not only decreases the stress induced by restraint and collection, but also allowsfor blood collection by a single technician. The method involves first conditioning felines at a younger age to be comfortable with venipuncture,toenail trims and veterinary treatments. Training begins at two days of age. At three weeks of age, the kittens are acclimated to being in a supineposition in a technician's lap with the sound of clippers running in the background. From this point, the technicians continue the supine positionplacement training while adding different collection techniques such as stretching the neck, stroking the jugular furrow, and clipping the hair alongthe furrow. This training is ongoing to ensure the safety of the felines and technicians. These training protocols and venipuncture method haveproven to be instrumental in improving feline well-being and technician aptitude in this research facility.



TAKE A TOURPoster Abstracts

Statistical Process for Qualification of Canine Panels for use in Digestible Energy Studies

Simple Environmental Enrichment Technique for Amphibian Species

Canine Intravenous Simulator Model

Scott M. Adams, Jeff H. Greene, Joe J. DiGennaroIAMS, Procter and Gamble

Quantifying the amount of protein that is readily available for absorption into the body is one indicator of overall diet quality. Thedecision to migrate from a metabolism cage to a more animal friendly environment (indoor/outdoor kennel) has affected the way wecollect data for determination of protein digestibility in pet food. Metabolism cages allowed for total fecal and urine collectionsthroughout the study. There is no practical method for collecting urine in an indoor/outdoor kennel, therefore a correction factor isnecessary. This shift to a “cageless” collection environment brought about some data variability and inconsistency, which needed to beaddressed.

Our challenge was to minimize inter-dog variability to deliver a more accurate prediction of protein digestibility in pet food. Factors suchas activity level and environmental stimuli (weather, temperature, human and dog interactions, etc.) now play a key role in the animals'metabolic process. The decision was made to evaluate protein digestibility over time, on a control diet, and to select animals whichdemonstrate both accuracy and repeatability. The data from a minimum of five repeat feedings per dog on the control diet was used toestablish a Protein MSE (mean standard error) value. This statistical calculation utilizes inter-dog variability and bias to generate thevalue necessary to rank the dogs on performance over time.

Carol Bluhm, CM, RLATG and Paula StilesFerris State University

Enrichment can be defined as “…a process for improving or enhancing animal environments and care within the context of their inhabitants'behavioral biology and natural history (AZA/BAG 1999). Though environmental enrichment is well illustrated throughout the literature formammalian species, examples for lower vertebrates is less well described.

A common misconception is that reptiles are stoic, adaptable and highly tolerant of the abnormal conditions of captivity. However, they mayactually be less tolerant and less adaptable to an unnatural, captive environment (Warwick, 1990, 1995). Unfortunately, much of the currentreptile husbandry and enrichment techniques have been derived from empirical information and not based on controlled studies.

Enrichment can be broken down into five different categories: food based, sensory, novel objects, social and training. We describe a simplesensory enrichment technique encompassing the five senses: sight, sound, touch, smell and taste. This housing technique closelyresembles the natural habitat for both turtles and frogs, is inexpensive to build and simple to maintain. While we have no empirical scientificdata to support the use of this technique, daily observational data comparing this enrichment technique with that used previously, suggestsanimals experience a better survival rate, reduced incidence of “redleg” and display less boredom type behavior.Although it is controversial,behavioral scientists believe abnormal behavior may be a form of stress manifested by over-reacting to novel or unexpected events,behavior noted using our previous housing model. This behavior has virtually been eliminated using the described enrichment environmentin both species.

Christopher Myers, BA, RLATGMPI Research

A simple, low cost and effective canine intravenous catheter simulator can easily be made almost entirely from supplies found in mostresearch facilities. The key to success is that the simulator accurately replicates the size and basic anatomy of the canine forelimb.Once assembled one can facilitate training on proper placement of catheters with this non-animal model. While total animalreplacement is not possible, the number of animals used will be decreasing and those animals that must be used will be more forrefinement of technique as opposed to learning the entire process thus reducing animal and trainee distress. In actively growingfacilities use of a simulator would also reduce the possibility of overuse of animals during times of increased hiring. Use of a simulatorwould also allow a facility to maintain a smaller overall colony and reduce the pinch on ever tightening budgets.

TOUR

Limited to 25 participants

Shuttle leaves Cherry Valley Lodge at 1:30 PM and Returns ~5 PMSterigenics is located 45 minutes from Cherry Valley Lodge.

http://www.sterigenics.comhttp://www.sterigenics.com/company/news/CNN_investigative_report

Sterigenics owns and operates a contract radiation sterilization facility in Westerville, OH. Cobalt-60 isutilized as the gamma radiation source with the primary purposes of general microbial reduction andsterilization of medical products.

BUILDING

The total building area is 20,000 square feet. Of this, the radiation cell area comprises approximately 2200square feet, and the office areas cover approximately 1300 square feet. The remaining building space is usedfor storage of isolated processed and non-processed product. The cell is constructed of reinforced concrete,approximately six feet thick. The system and irradiation cell were both designed and constructed bySteriGenics International.

IRRADIATION SYSTEM DESCRIPTION

The irradiation system is a multi-pass/multi-shelf product overlap design. Product is exposed to sealedsources of Cobalt-60 which emit gamma rays. A highly automated conveyor system is used for transportingproduct through the system. The product is loaded into containers known as “totes”. These totes are thenautomatically loaded onto multi-tiered carriers. Each carrier holds three totes. Material which is loaded ontothe input conveyor via a tote is carried through the cell on a carrier and is eventually unloaded onto the outputconveyor.

During system operation, the source racks containing the Cobalt-60 are above ground and adjacent to thecarriers. When access to the cell is required, the racks are lowered to the bottom of the cell pool.

We will be given a tour of the facility and a demonstration of the irradiation process.You may find it interesting that Purina sends Lab Diet to be irradiated at this plant.


TAKE A TOURPoster Abstracts

Statistical Process for Qualification of Canine Panels for use in Digestible Energy Studies

Simple Environmental Enrichment Technique for Amphibian Species

Canine Intravenous Simulator Model

Scott M. Adams, Jeff H. Greene, Joe J. DiGennaroIAMS, Procter and Gamble

Quantifying the amount of protein that is readily available for absorption into the body is one indicator of overall diet quality. Thedecision to migrate from a metabolism cage to a more animal friendly environment (indoor/outdoor kennel) has affected the way wecollect data for determination of protein digestibility in pet food. Metabolism cages allowed for total fecal and urine collectionsthroughout the study. There is no practical method for collecting urine in an indoor/outdoor kennel, therefore a correction factor isnecessary. This shift to a “cageless” collection environment brought about some data variability and inconsistency, which needed to beaddressed.

Our challenge was to minimize inter-dog variability to deliver a more accurate prediction of protein digestibility in pet food. Factors suchas activity level and environmental stimuli (weather, temperature, human and dog interactions, etc.) now play a key role in the animals'metabolic process. The decision was made to evaluate protein digestibility over time, on a control diet, and to select animals whichdemonstrate both accuracy and repeatability. The data from a minimum of five repeat feedings per dog on the control diet was used toestablish a Protein MSE (mean standard error) value. This statistical calculation utilizes inter-dog variability and bias to generate thevalue necessary to rank the dogs on performance over time.

Carol Bluhm, CM, RLATG and Paula StilesFerris State University

Enrichment can be defined as “…a process for improving or enhancing animal environments and care within the context of their inhabitants'behavioral biology and natural history (AZA/BAG 1999). Though environmental enrichment is well illustrated throughout the literature formammalian species, examples for lower vertebrates is less well described.

A common misconception is that reptiles are stoic, adaptable and highly tolerant of the abnormal conditions of captivity. However, they mayactually be less tolerant and less adaptable to an unnatural, captive environment (Warwick, 1990, 1995). Unfortunately, much of the currentreptile husbandry and enrichment techniques have been derived from empirical information and not based on controlled studies.

Enrichment can be broken down into five different categories: food based, sensory, novel objects, social and training. We describe a simplesensory enrichment technique encompassing the five senses: sight, sound, touch, smell and taste. This housing technique closelyresembles the natural habitat for both turtles and frogs, is inexpensive to build and simple to maintain. While we have no empirical scientificdata to support the use of this technique, daily observational data comparing this enrichment technique with that used previously, suggestsanimals experience a better survival rate, reduced incidence of “redleg” and display less boredom type behavior.Although it is controversial,behavioral scientists believe abnormal behavior may be a form of stress manifested by over-reacting to novel or unexpected events,behavior noted using our previous housing model. This behavior has virtually been eliminated using the described enrichment environmentin both species.

Christopher Myers, BA, RLATGMPI Research

A simple, low cost and effective canine intravenous catheter simulator can easily be made almost entirely from supplies found in mostresearch facilities. The key to success is that the simulator accurately replicates the size and basic anatomy of the canine forelimb.Once assembled one can facilitate training on proper placement of catheters with this non-animal model. While total animalreplacement is not possible, the number of animals used will be decreasing and those animals that must be used will be more forrefinement of technique as opposed to learning the entire process thus reducing animal and trainee distress. In actively growingfacilities use of a simulator would also reduce the possibility of overuse of animals during times of increased hiring. Use of a simulatorwould also allow a facility to maintain a smaller overall colony and reduce the pinch on ever tightening budgets.

TOUR

Limited to 25 participants

Shuttle leaves Cherry Valley Lodge at 1:30 PM and Returns ~5 PMSterigenics is located 45 minutes from Cherry Valley Lodge.

http://www.sterigenics.comhttp://www.sterigenics.com/company/news/CNN_investigative_report

Sterigenics owns and operates a contract radiation sterilization facility in Westerville, OH. Cobalt-60 isutilized as the gamma radiation source with the primary purposes of general microbial reduction andsterilization of medical products.

BUILDING

The total building area is 20,000 square feet. Of this, the radiation cell area comprises approximately 2200square feet, and the office areas cover approximately 1300 square feet. The remaining building space is usedfor storage of isolated processed and non-processed product. The cell is constructed of reinforced concrete,approximately six feet thick. The system and irradiation cell were both designed and constructed bySteriGenics International.

IRRADIATION SYSTEM DESCRIPTION

The irradiation system is a multi-pass/multi-shelf product overlap design. Product is exposed to sealedsources of Cobalt-60 which emit gamma rays. A highly automated conveyor system is used for transportingproduct through the system. The product is loaded into containers known as “totes”. These totes are thenautomatically loaded onto multi-tiered carriers. Each carrier holds three totes. Material which is loaded ontothe input conveyor via a tote is carried through the cell on a carrier and is eventually unloaded onto the outputconveyor.

During system operation, the source racks containing the Cobalt-60 are above ground and adjacent to thecarriers. When access to the cell is required, the racks are lowered to the bottom of the cell pool.

We will be given a tour of the facility and a demonstration of the irradiation process.You may find it interesting that Purina sends Lab Diet to be irradiated at this plant.

Selecting the Right Decontaminating Agent for Rooms

What is Clean Anyway? IVC Cleaning Practices

Is it Time to Replace Your Newsletter with a Blog?

Blood Collection from the Lateral Metatarsal Vein in the Guinea Pig

Mark Czarneski / Paul LorcheimClorDiSys Solutions Inc.

Peggy NahorskiAlternative Design Mfg

Chris LyonsAssociate Executive Director, AALAS

Dr. Laurie Goodchild and Kelly Brown, BS, LATG, VTThe Research Institute at Nationwide Children's Hospital

This presentation describes and compares the primary methods of sterilizing / decontaminating clean rooms. The methods considered includegaseous systems, vapor systems, misting systems, and fogging, as well as manual spray and wipe techniques. The goal is to give the listeners anunderstanding of what the benefits and drawbacks of each method are so that an educated decision can be made as to what methods are bestsuited for different required levels of decontamination. It describes the penetrating ability of gaseous methods, the simplicity of spray and wipetechniques, the cost effectiveness of fogging and formaldehyde gassing, and issues of “wet” vapors versus “dry” vapors.The practical aspects of the different methods are also compared and contrasted. Room size and configuration limitations are discussed. Thepractical aspects of empty rooms versus “cluttered” rooms are also considered.This presentation is unique in that in compares all major methods, systems, and techniques in their effectiveness, ease of use, relative cost, andpractical application. The significance of this is that by comparing the various methods, the proper method can be utilized for the desired level ofdecontamination that the user wants and requires for each particular application.

Presentation reviews questions vivarium managers need to ask when developing proper cleaning and sanitizing processes for vent racks (IVCs).How important is it to clean inside a vent rack and how easy is it to do, will be some of the questions addressed. The presenter will then illustratewhat some vent rack manufacturers are doing to make cleaning IVCs easier and more efficient with facility washing equipment.

The challenges of publishing a Branch newsletter include finding and teaching someone to lay out articles, dealing with printer and mailinghassles, updating mailing addresses all the time, and maintaining funds for printing and mailing. Well maybe now's the time to consider replacingthe newsletter with a blog. The blog platform continues to evolve and is ideal for resource sites and newsletters. In this presentation, discover howa blog can save you money, keep your members informed, save you time, and generate advertising revenue for your Branch.

The Animal Rule

In-vitro Fertilization for Mouse Colony Management

Assisting Transgenic Production - Maximize Mouse Embryo Yield by Superovulation

Jennifer Quick Kerns, AS, RVT, RLATG, RQAP-GLPBattelle Biomedical Research Center

Xin-an Pu, PhD

The Ohio State University

Ming LuThe Ohio State University

New to the lab animal world and the FDA is a new set of regulations by the FDA known as the "Animal Rule". 21 CFR Parts 314 and 601 arenewer regulations that are still under development. To be used in combination with 21 CFR Part 58, the Good Laboratory Practices, this set ofregulations has been developed to provide evidence needed to demonstrate effectiveness of new drugs when human efficacy studies are notethical or feasible. The FDA may approve a product for which human safety has been established, and the results of adequate and well-controlledanimal studies establish that the product is reasonably likely to provide clinical benefit in humans. This can only be used if human clinical efficacytrials are not feasible or are not ethical. This set of new regulations is sure to change the laboratory animal world forever.

Tech TipsPoster Abstracts (continued)

Comparison of Feline Reproduction Success: Female Presented in Male's Home Environment vs. Male Presented in Female'sHome Environment

The Positive Impact of P&G Pet Care's Early Feline Socialization Program

Leann King, Deron SmithP & G Pet Care

Kristina Grider, Deron SmithP & G Pet Care

The P&G Pet Care Pet Health and Nutrition Center (PHNC) Cat Colony implemented a breeding program to maintain our specific pathogen-freestatus. In the PHNC environment, it would be advantageous to bring the male to the female's home for mating due to our housing requirements.This is not the traditional method based on the concern of how the male will perform in a new environment. In feral cat populations, the tom'sterritory will overlap the queen's territory. The tom will essentially travel into the queen's home territory. The belief is that taking the male to thefemale would allow the program to feel more natural for the animals and have equivalent or better reproduction results. The poster will outline themating processes used and the findings.

The P&G Pet Care Pet Health & Nutrition Center Cat Colony breeding program was designed to produce healthy kittens that are well socialized,easily adapt to change in the research environment, and are readily adoptable.

The key components of the program that will be presented include the following:

Kitten-to-Human and conspecific socialization.Kitten training using positive reinforcement and desensitization techniquesKitten acclimation methodology for procedures such as healthcare restraint techniquesIntroduction to environmental stimuli (i.e. Olfactory scents, sounds, textures, diets)

The positive impact of the Early Socialization Program will also be subjectively presented in the poster. Also, plans for objective measures will beoffered.

Innovative Venipuncture Method for the Feline SpeciesCindy LanmanP & G Pet Care

In every capacity of research and in every facility, it can be assumed that methodology refinements come to the minds of technicians quite often. Inthe case of feline restraint for blood collections, new ways to reduce the stressful nature of these procedures for both the felines and the technicianshave been difficult to uncover. This innovative venipuncture method not only decreases the stress induced by restraint and collection, but also allowsfor blood collection by a single technician. The method involves first conditioning felines at a younger age to be comfortable with venipuncture,toenail trims and veterinary treatments. Training begins at two days of age. At three weeks of age, the kittens are acclimated to being in a supineposition in a technician's lap with the sound of clippers running in the background. From this point, the technicians continue the supine positionplacement training while adding different collection techniques such as stretching the neck, stroking the jugular furrow, and clipping the hair alongthe furrow. This training is ongoing to ensure the safety of the felines and technicians. These training protocols and venipuncture method haveproven to be instrumental in improving feline well-being and technician aptitude in this research facility.


Environmental Enrichment for Cats Lessons Learned from a Stress-sensitive Population

Coyote Vocalization

Visceral Larval Migrans

Dr. James Buffington and Judi StellaThe Ohio State University

Karen HallbergThe Ohio State University

Dr. GehrtThe Ohio State University

Dr. WynnThe Ohio State UniversityRattlesnake Husbandry

Mr. WinslowStone Laboratory

Our laboratory studies “FUS” in cats. Previously known as “feline urologic syndrome” the term is now known to describe a “feeling unexplainedsyndrome”. Our studies have found that these cats appear to have a developmental disorder that leads to a sensitized stress response system.Physiological consequences of this sensitization result in abnormalities of a variety of body systems, and the destruction of millions of cats in theUS each Year. It also is a naturally occurring disease analog of an idiopathic pain syndrome in humans called interstitial cystitis. Although usuallynot lethal (notwithstanding regular reports of suicide among sufferers), the disease can lead to a lifetime of suffering.

During the past few years we have documented the stress sensitivity of these cats, and found that we can resolve clinical signs (but not thedisease) by environmental enrichment (EE). Based on these studies, we have improved clinical approaches for therapy of client-owned cats.( )

During this presentation we will present our findings related to neural, endocrine and immune abnormalities in these cats, our approaches to EE,and discuss some of the mechanisms that may underlie the effects of EE in cats, as well as in other species. We also will present data supportingthe idea that good husbandry, EE and low stress environments benefit the animals, the researchers and the animal care staff. These benefitsinclude increased ease of handling, cage hygiene, and reduced sickness behaviors.Questions from and discussion are welcome so we all can learn from our interaction.

Karen Hallberg is currently a PhD Candidate in the Department of Evolution, Ecology, & Organismal Biology (EEOB) at The Ohio State University.Her dissertation research investigates the role of chorus howling in coyote social and territorial strategies.

Karen completed her MA in Psychology, in the area of psychobiology, at Ohio State in 2000 and transferred to the Department of EEOB in 2001.Having studied captive primates in her bachelor's and master's programs, Karen sought to explore the behavior of animals in their natural habitatfor her PhD.

Although her graduate research has focused on questions of animal behavior and bioacoustics, her work with wild coyote populations has ledKaren to develop a keen interest in issues of human-wildlife conflict and the potential of public education to promote coexistence.

Www.indoorcat.org


DON’T FORGET!

Poker RunApril 30

1:30 p.m.$5 ♠



Evaluation of Non-human Primate Environmental Enrichment Program

Rat Neonate Oral Gavage and Intravenous Dosing Procedures

Catheterization of the Medial Artery in Rabbits

Colors of the StripUsing Color for Monthly Room Logs

Douglas J. ChieffeCleveland Clinic

Julie Roller and Linda UnrueCharles River Laboratories Preclinical Services Ohio

Julie Roller and Erica ConawayCharles River Laboratories Preclinical Services Ohio

Kathy Hopwood and Shannon WellsThe Ohio State UniversityUniversity Laboratory Animal Resources

Wild primates live in very complex social environments and are constantly looking for food, water, and mates. These wild primates are stimulated allday. In the laboratory setting there needs to be complete control over the subjects of a study, making it impossible to recreate the naturalenvironment of a primate. Studies have shown that under-stimulated primates may suffer from stress which can cause physiological problems. Usingprimates with physiological problems in research studies as a model of normal function is not a reliable source of information.

The Animal Welfare Act requires that laboratory animals be provided with environmental enrichment to promote psychological well-being.Environmental enrichment includes novel foods and toys that give the primates stimulation. The longer a monkey manipulates the enrichment, thebetter because it is stimulating the monkey for longer. The Cleveland Clinic Foundation provides its monkeys with many novel foods and toys.

The idea for this poster comes from projects that were worked on at Charles River Laboratories, Ohio Division. The technicians involved had todevelop a method and procedure for oral gavage and bolus intravenous dosing on rat pups ranging in age from 7 10 days old. The poster is goingto outline the materials utilized to accomplish this very difficult dosing technique. An illustration of each technique is also going to be supplied, as wellas some helpful hints for successfully accomplishing each route of dosing.

The idea for this poster comes from projects that were worked on at Charles River Laboratories, Ohio Division. The technicians involved had todevelop a method and procedure for inserting and maintaining a catheter in the medial artery of a New Zealand White rabbit. The poster will illustratethe placement of the catheter, as well as the materials utilized. The procedure for placing the catheter will be explained, as well as the procedurefollowed to maintain the patency (we have kept catheters patent for up to 12 hours). A sample blood collection regimen will be included with helpfulhints for scheduling and collection of samples. Safe removal of the catheter will be discussed, as well as some pointers for a successfulcatheterization.

Often in monthly room logs the days and weeks seem to blend together. It is usually hard to keep track of where a week ends and another begins.Using color coding, we have simplified the monthly room logs. We are using different colors for each set of weekly requirementsthere are tasks thatneed to be done three times per week, two times per week, and every two weeks. It is easier for the husbandry employees and supervisors to seewhich tasks need to be completed, which have already been completed, and what has not yet been completed each week. The poster will displaythe original form, the new colored form and the advantages for the new system.

Rabbit Group HousingEboni Lewis, B.S. RALAT, JoeAnn Croxford, A.A. RLATG, Margaret A. Hankes-Pena, RLATGUniversity of Kentucky

Animal facilities generally house their rabbits in standard caging. The size of the caging, as outlined in The Guide For the Humane Care and Use ofLaboratory Animals specifies the minimum square footage allotted to individually housed rabbits both in area and height, but does not take intoaccount the natural behavioral traits of the animal. It does address, however, environmental enrichment and states that it is desirable for socialanimals such as rabbits to be group housed when possible. (Continued on next page.)

Macaque Retrovirus Infections: Potential Confounding Variables in Biomedical Research

Playing Possum with the Ohio Wildlife Center

Animal Biosafety Levels Explained: Something for Everyone

Ferret Husbandry, Enrichment, Anesthesia and Intratracheal Dosing

Animal Models of Tuberculosis

Coping Mechanisms for Pet Loss/Bereavement

Veterinary Technicians: Career Options (Private Practice, Research)

Alternative Searches and Resources AWIC has for the Research Community


Mike SeidelOhio Wildlife Center

Robin Trundy, MS, CBSPBattelle Biomedical Research Center

John W. Merrill, BS, LATGBattelle Memorial Institute

Christian E. Newcomer, VMDJohns Hopkins

Jennifer Brandt, MSW, LISW, PhDThe Ohio State University

Pamela SniderColumbus State Community College

D'Anna JensenTechnical Information SpecialistAnimal Welfare Information Center (AWIC)

It is well recognized that adventitious infectious agents can interfere with biomedical research by increasing morbidity, mortality, and variability inscientific data, thus confounding research. Some infectious agents are acute and self-limiting, while others cause persistent or latent life-longinfections. Among the group of agents that cause life-long infections are the retroviruses. Retroviruses are a large and diverse group of enveloped,single stranded RNA viruses that are unique among viruses they possess a complement of enzymes, including reverse transcriptase, that allowsthem to reverse transcribe their RNA genomes into DNA and then insert that DNA into the host's chromosomal DNA. Once the retrovirus isinserted into the host's genome it is called a DNA provirus. The integrated DNA provirus allows retroviruses to persistently infect their host and toavoid surveillance by the host's immune system. Retroviruses can infect a wide variety of cells within their host, often including cells of the immunesystem. Retrovirus infections can be subtle and subclinical, or they can result in debilitating immunosuppression which makes animals susceptibleto a wide variety of opportunistic infectious agents and cancers.There are currently four well described retroviruses known to infect laboratory macaques: simian immunodeficiency virus (SIV), simian type Dretroviruses 1-5 (SRV), simian T-lymphotropic virus (STLV) and simian foamy virus (SFV). In this presentation we will discuss the pathogenesis ifeach of these agents including diagnosis, and potential to impact biomedical research and colony health.

Presenting Ohio's native wildlife with characteristics of habitat, enrichment, etc. The presentation will field questions and discuss overall wildliferehabilitation and release here at the Ohio Wildlife Center.

A biosafety level is a combination of facilities, equipment, and practices designed to allow personnel to work with biohazardous material in amanner that is safe for them, and safe for the surrounding environment. When the biohazardous material is used in an animal model, there arespecial considerations that surpass issues that might arise in a typical lab setting. Depending on the nature of the biohazardous material (wild typeinfectious agents, agents that contain recombinant DNA molecules, etc.) and the species of the animal, different guidelines relative to biosafetylevels will apply. This presentation will review the definition of biosafety levels and describe the unique features of animal-related biosafety levelsunder different regulatory requirements.

Presentation Abstracts (continued)Poster Abstracts (continued)


Rabbit Group Housing (continued)

Reliable Old Caging Is NotAlways a Safe Bet

To this end at the University of Kentucky, Division of Laboratory Animal Resources (DLAR) we looked at group-housing as an alternative to individualcaging. Our goal was simple, to improve the quality of care and provide a group setting for short and long term rabbits, while still being able to isolatethe individual. Group housing rabbits provides increased space to allow them freedom of movement to carry out normal activities such as hopping,stretching out, sitting up with erect ears, rearing and leaping. Rabbits are naturally gregarious and group-housing allows them to have socialinteractions such as grooming, playing, and lying together. It also offers greater opportunity for environmental enrichment such as foraging and theability to have multiple levels within the environment. Another benefit of group/pen housing was increased activity to help counteract the forcedsedate life style of long term animals by providing opportunities to exercise. This increases appetite, decreases obesity, helps prevent conditionssuch as “sore hocks”, hairballs, and diarrhea) and adds to overall general health.

An intact male dog was presented with three approximately 1cm lesions of unknown origin on the anterior aspect of its scrotum. The lesionsappeared somewhat raised and moist but were not obviously infected. Upon further observation, the dog was viewed jumping up at the front gate ofits run causing the scrotum to come into momentary contact with an obscure recessed area 19 inches above the floor on the gate of the run. Theseruns are in compliance with USDA regulations and have been used uneventfully for over 20 years.

The obscure recessed area is a groove designed to shelter the ends of the vertical bars in gate from contact with the dog and is in direct line with thespecific area of the dog's scrotum when he was standing on his hind limbs. The lesions were the result of low grade trauma due to contact from thisarea of the gate. To prevent further trauma the groove was filled with silicone caulk so that the ends of the bars were covered. The lesions wereinitially treated with a 10% betadine solution and silver sulfadiazine ointment. Following initial treatment and the application of silicon caulk, thelesions resolved uneventfully.

While this type of lesion had never been observed on the hundreds of dogs housed in these runs over 20 years, it demonstrates that there are alwaysodds that reliable old caging is not always a good bet.

Canine Home Environment Acclimation Program (A Conditioning Program to Better Prepare Research Dogs for Adoption)

Temperature and Humidity Alterations Due to Caging Type

Jennifer L. Wheatley, Gwyn L. Jeans, Kevin D. Corcoran, Andrew S. FixProctor and Gamble

Lindsay Zinser, Greg BoivinCincinnati VA Medical Center

Many canines retiring from research service are deemed eligible for adoption. In an effort to better prepare these dogs for a successful transition to ahome environment, we describe an acclimation program implemented at The Procter and Gamble Company. This program includes a specificacclimation and training plan for each dog with exposure to environments and stimuli likely to be encountered post-adoption. Our goal is to addressany existing behavioral or socialization issues, introduce housebreaking principals, and familiarize dogs with common household items prior toadoption. Acclimation within an artificial home environment and other interventions are provided based on individual animal needs. We have foundthis program to be beneficial and enriching not only for the canine subjects, but also for the animal care and veterinary staff members who haveparticipated. The program has been utilized for many dogs which are now successfully placed in homes. As a way to improve the transition to ahome environment for canine adoption candidates, while increasing job satisfaction for employees, we advocate consideration of an acclimationprogram such as this.

The Guide for the Care and Use of Laboratory Animals states “The relative humidity should generally be maintained within a range of 30-70% throughoutthe year, and the recommended dry bulb temperature for mice and rats is between 64 and 79° F.” With the advent of new caging types, standard monitoringof room data may be insufficient to truly evaluate the local rodent environmental conditions. In this study we examined the effect of different housingconditions for mice and rats and compared temperature and humidity with room values. Mice housed in PIV cages had on average an increase in cagetemperature of 7.2°F and a 4.5% decrease in humidity. Both values were significant (p<0.0001). Mice and rats in conventional shoebox cages withmicrofilter tops had significant increases (p<0.001) in both temperature and humidity (mice 3.7°F & 6.3%; rats 3.1°F & 3.4% respectively). The number ofanimals in the cage (all below Guide space requirements), significantly alter the changes identified. Increasing biomass in the cage increased thetemperature and humidity proportionally. Despite increased air flow, the PIV racks consistently had higher temperatures than static isolator cages. Werecommend that these changes in temperature and humidity be considered when establishing room parameters. In addition, extreme care must beobserved when room temperatures increase due to HVAC failure because the in cage temperatures are significantly higher than the room temperature.

Candace HedrickThe Ohio State University, University Laboratory Animal Resources

Public Health Issues in Laboratory Animal Populations

Increased Operating Efficiencies Translate into Decreased Utility and Labor Costs

Improvements to the Dog Bile Cannulation Surgery Model

Murine Norovirus in Laboratory Mice

Noé Tirado-Muñiz, DMV, DVM, MSBattelle Memorial Institute

Jori Leszczynski, DVMNational Jewish Medical and Research Center

Oscar A. Bermeo Blanco, DVM, RLATGBattelle Memorial Institute


Although not as common as in the past, zoonotic diseases (diseases transmissible from animals to humans) still play an important role in our dailyactivities. Whether you are working with wild-caught animals, immune- compromised animal models, animal models of infectious diseases or othersettings, the knowledge of zoonotic disease control and prevention is of paramount importance for your safety and the safety of coworkers. We willreview simple but key aspects of zoonotic disease transmission prevention as well as a few cases of public health interest.

There are many ways that an animal facility can decrease costs to the institution. Over the past year, National Jewish has undertaken a cage washremodeling plan that included aggressive evaluation of water use. Eventually, equipment purchases were partially determined by their operatingefficiency. This has translated into thousands of dollars in initial incentives from the Denver Water Department and thousands of dollars each year inutility savings. In addition, National Jewish has also undertaken personnel initiatives such as flex-time and multi-faceted job responsibilities thathave saved money on overtime and turnover, as well as anectodatally increased employee satisfaction.

Successful canine bile cannulation surgery is critical to the performance of pharmacodynamic studies in preclinical toxicology. This presentation willdemonstrate improvements which enhanced the performance and longevity of the dog bile cannulated model. The lessons learned from previousmodels helped to refine bile collection techniques with minimal physiological effects to the animal. Topics to be discussed include acclimation to feed,collars and jackets prior to surgery, the surgical procedure itself and post-operative care. Don't gamble on the success of a surgicalmodelpreparation is the key!

Murine norovirus (MNV) is a newly emerging mouse that was first described by Stephanie Karst and colleagues in 2003. They reportedthat mice with severe deficiencies in their innate immune systems, specifically , died sporadically after infection by MNV-1. This sparked agreat deal of interest in MNV, since norovirus infections are reported to be responsible for 90% of human nonbacterial gastroenteritis, worldwide, andthere are no small animal models of human norovirus infection.

Noroviruses belong to a large and diverse group of nonenveloped RNA viruses that, in addition to humans, infect a variety of animals including cats,rabbits, pigs, sea lions and cattle. MNV has created both interest and consternation within the laboratory animal community because its prevalencein contemporary mouse colonies, as assessed by published reports from research animal diagnostic laboratories, ranges from 22-29% of the micetested: indicating that MNV is by far the most prevalent virus in laboratory mouse colonies. To date, MNV field strains have not been associated withsigns of clinical disease in immunocompetent mice; however, MNV-1 does infect macrophages, a key component of the immune system.

In this talk we will discuss the pathobiology of MNV infections in laboratory mice, include the biology of the and the discovery,pathogenesis, diversity and biology of MNV. Current methods for molecular and serologic detection of MNV will be covered and the potential impactof MNV on biomedical research will be discussed.

Calicivirus

STAT-1

Calicivirdae


Veggie Tails: A Novel Training Method

Strategies for the Elimination of Mouse Parvovirus

Adoption as an Alternative Destiny for Retiring Research Dogs

Christina Zehender; Joshua HittleCharles River Laboratories Preclinical Services Ohio

Marie Koenig, Gerald SmithEli Lilly and Co.

Andrew S. Fix , Jennifer L. Borger , Robin Cooper , Gwyn L. Jeans ,Becky R. Rupert , Mark A. Tetrick , Jennifer L. WheatleyP&G Central Product Safety P&G Pet Care

The lateral tail vein is a common method of administering test material in rat models, primarily in toxicology research. Learning and becomingproficient at the procedure can be difficult for technicians and place stress on practice animals. We propose using an alternative method of learningthe procedure on a non-animal model. Celery offers a great alternative to rat models due to the vascular tissue running along the stalk of the plant.Injection of methylene blue into the vascular tissue of celery allows a technician to properly align the syringe and place the needle at the properdepth into the plant tissue as per proper tail IV functions. The injection of methylene blue also allows the technician to visually confirm properplacement of the syringe, as the compound will travel through the celery vascular tissue. These new procedures with reduce the amount of practiceanimals in the laboratory and reduce the amount of stress on the animals.

Eliminating mouse parvovirus (MPV) from our continuously occupied multi-user facility has been difficult. Sentinels (Hsd: ICR, CD-1 mice) weregroup-housed in non-filtered polycarbonate cages on room floors, exposed to soiled bedding weekly and sampled every 6 wk. Detection sensitivityby the sentinels was likely compromised by a low prevalence of infection in resident mice and poor transmission to sentinels. From 2001 to 2005, oursentinel program indicated 30% of our rooms had a history of MPV-positive sentinels. The incidence of MPV was essentially unchanged during theperiod of time, in spite of depopulation and decontamination of MPV-positive rooms. Specific epidemiological traits were observed in these rooms;the eradication strategy was modified to focus on rooms with these traits. MPV-positive rooms usually had one of the following characteristics:breeding colonies, multiple users, or rooms where mice were frequently transferred to or from testing laboratories outside the vivarium. Therefore,the strategy was modified to periodically depopulate and decontaminate rooms with these characteristics, regardless of negative sentinel results.Advance notice was given to researchers to stop receiving animals into a room scheduled for decontamination and to complete their studies in thatroom. The schedule was flexible to avoid interfering with research projects. Since MPV persistence is also affected by its resistance to environmentalinactivation, decontamination procedures were modified from liquid chemicals only to an additional method for decontaminating rooms, laboratoriesand equipment using vaporized hydrogen peroxide (VHP). VHP is a process that maintains vapor concentration below the condensation point toprotect sensitive instrumentation. The number of breeding rooms was also decreased. Subsequently, MPV infections were dramatically reduced.Since 2005, 18 mouse rooms and 18 laboratory/procedure rooms were depopulated and VHP-decontaminated. Incidence of MPV-positive rooms hasdecreased to about 14% since 2005, and sentinels have been MPV-negative for 9 mo.

Adoption is a viable destiny alternative for animals involved in some, but not all, types of research service. We describe an adoption program fordogs implemented at The Procter and Gamble Company. This program included: 1) a set of principles for adoption, with inclusion/exclusion criteria,2) an adoptability classification system based on thorough medical and behavioral assessments, 3) a specific acclimation and training plan for eachdog, and 4) a screening and approval process for prospective owners. Adoption candidates were dogs from a wide age range and service history.Dogs received complete pre-adoption assessments and were grouped according to the adoptability classification system. This helped identify areasto address during pre-adoption acclimation and training. Specific training or other interventions were provided based on individual needs, includingleash training and exposure to environments likely to be encountered post-adoption. A number of dogs were moved through this program andsuccessfully placed in homes. Implementation of this program required the collaborative efforts of staff from the veterinary, animal welfare, andfacility operation areas. As a way to improve the quality of life of retired research dogs and decrease the number of animals euthanized in non-terminal research, we advocate broader consideration of active intervention programs and adoption as an alternate destiny for these animals.

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Preliminary Meeting Presentation Schedule We Are Betting

You Can Be The Winner!

The District 5 2008 AALAS meeting planning committee would likeyou to participate in a silent auction.

The auction closes at 5:00 PM Thursday and the highestbidders will be announced at the banquet Thursday night.

If you wish to donate items please contact:

At Our

During the

Individuals, vendors, and local branches are invited to donate itemsto be auctioned.

Jennifer Quick-Kerns, Battelle Memorial Institute, 505 King Ave., JM-7-110,Columbus, OH 43201. Email: . Phone: 614.424.5468

Silent Auction

[email protected]

District 5 AALAS MeetingApril 30 -May 2 , 2008

Newark, Ohio

th nd

♠

♠

♠

8:30 AM – 8:45 AM Opening Remarks

Scientific Technical Special Topics Workshops

8:45 AM – 9:30 AMBill Winsley

‘Controlled Substances:Licensing, Use and

Storage’

Karen HallbergCoyote Vocalization 8:45 AM – 9:45 AM

BASi Workshop 1


9:45 AM – 10:30 AMDr. Gehrt

‘Visceral LarvalMigrans’

Tech Tips:-Mark Czarneski &

Paul Lorcheim-Peggy Nahorski

-Dr. Laurie Goodchild& Kelly Brown-Chris Lyons

Christopher J. WinslowStone Labs

‘Aquatic Research’

10:45 AM – 11:30 AM

Dr. Noé Tirado-Muñiz‘Public Health Issues in

Laboratory AnimalPopulations’

Pam Snider‘Careers for Veterinary

Technicians’

Mike Seidel‘Playing Possum with

the Ohio WildlifeCenter’

10:15 AM – 11:15 AMBASi Workshop 2


11:30 AM - 1:00 PM LUNCH

1:15 PM – 2:00 PM

D’Anna Jensen‘Alternative Searchesand Resources AWIChas for the Research

Community’

Dr. Wynn‘Rattlesnake Husbandry’

2:15 PM – 3:00 PM

Dr. Tracy Peace‘Holding and Playing

the Right Cards Duringan AAALAC Site Visit’

Dr. Tony Buffingtonand Judi Stella‘Environmental

Enrichment for Cats:Lessons Learned from

a Stress-sensitivePopulation ’

3:15 PM – 4:00 PM

Dr. Joe Simmons‘Update for Murine

Noroviruses andPrimate Retroviruses’

Dr. Jori Leszczynski‘Increased OperatingEfficiencies Translateinto Decreased Utility

and Labor Costs’

Tech Tips:-Jennifer Quick-Kerns-Dr. Xin-an Pu-Ming Lu

Friday, May 2

9:00 AM – 9:45 AM

Dr. ChristianNewcomer

‘Animal Models ofTuberculosis’

Dr. Oscar A. BermeoBlanco

‘Improvements to theDog Bile Cannulation

Surgery Model’

Jennifer Brandt‘Coping Mechanisms forPet Loss/Bereavement’

9:50 AM – 10:45 AM

Robin Trundy'Animal BiosafetyLevels Explained:

Something forEveryone'

John Merrill‘Ferret Husbandry,

Enrichment,Anesthesia and

Intratracheal Dosing’

9:00 am – 11:00 am

A Program for SimulatedRodent Surgical Training


♠ ♠

♠ ♠

Wednesday April 30Golf Outing

Registration

Exhibit Set-Up

Poster Set-Up

Sterigenics Tour

Poker Run

Welcome Party “Casino Night”

Registration


Exhibit Set-Up

Poster Set-Up

9:30 AMRaccoon International Golf CourseGranville, Ohio

12:00 PM - 5:30 PMCherry Valley Lodge Lobby

12:00 PM - 5:30 PMJubilee Rms A-E

12:00 PM - 5:00 PMFoyer outside Blossom Rms I-IV

1:30 PMSterigenicsWesterville, Ohio

1:30 PMCherry Valley Lodge Lobby

6:00 PM -10:30 PMBlossom Rms III and IV

7:30 AM - 5:00 PMCherry Valley Lodge Lobby


7:30 AM - 8:00 AMJubilee Rms A-E

7:30 AM 8:00 AMFoyer outside Blossom Rms I-IV

Thursday May 1

First Timers Breakfast7:30 AM 8:30 AMBlossom Rm IV

♠ ♠Friday May 2Registration


District 5 Council Breakfast

TBR Breakfast


7:30 AM 9:00 AMCherry Valley Lodge Lobby





♠ ♠Thursday May 1(Continued)

Poster Session Open

Silent Auction

Opening Remarks


Box Lunch


Poster Authors Present/Poster Judging

Cocktail Hour

Awards Banquet and Social

8:00 AM 5:00 PMFoyer outside Blossom Rms I-IV

8:00 AM - 5:00 PMFoyer outside Jubilee Rms A-E

8:30 AM- 8:45 AMBlossom Rm IV


11:30 AM -1:00 PMCherry Valley Lodge

1:15 PM 4:00 PMBlossom Rms I-IV

4:00 PM 5:00 PMFoyer outside Blossom Rms I-IV

6:00 PM- 7:00 PMCherry Valley Lodg

7:00 PM 9:00 PMJubilee Rms A-E

e

Exhibits Open8:00 AM 5:00 PMJubilee Rms A-E

♠See You

Next Year!2009 District 5 Meeting

The Galt House Louisville, KY


Preliminary Schedule of Events

TECHNICIAN FUN FAIR

Join us for

in the vendor area!fun and excitement

Test your knowledge of vendors and their products, AALAS,and the 2008 District 5 Program

Talk with vendors and committee members to find their answers. You can also use the meetingprogram.

Correct answers make you eligible for a drawing held at the Thursday night banguet.

All participants will receive a certificate suitable for framing



District 5 AALAS 2008 Annual Meeting“Bet Your Life on Research”

April 30 – May 2, 2008Cherry Valley Lodge

Newark, OH

Presenter Registration Form

First Name Last Name Credentials/Degrees:

Institution

Mailing Address

City State Zip

Telephone Fax Email

Presentation Title

Audio Visual Requirements (Check All Required): Slide Projector PowerPoint Projector & PCOverhead Projector Other

Registration Fees (Final Payment due April 4th)Cancellation/Refund Policy: D5 registration fees are refundable through 4-16-08 minus a $25 processing fee!

Options *Fee* Total

Registration WaivedWelcome Reception April 30th WaivedMealsIndividual Lunch: offered only Thursday $20Individual Dinner: (check one) Wednesday, Thursday, both $40WorkshopsBASi “Culex Automated DMPK in Vivo Sampling”(check one) Thursday 8:45am-9:45am 10:15am-11:15am

$10

“A Program for Simulated Rodent Surgical Training”Friday morning

$10

ToursSterigenics Facility Tour – limited to the first 25 registrants $10Poker Run: See additional form – register onsite feeGolf Outing: has a separate form – mail to Dale Ettel Total

� Two reduced fee registrations are available as scholarships for individuals that may need financial assistance inorder to attend. For details contact LaTonya Mitchell: [email protected]. Apply by March 14th, 2008.

Do Not Send Cash:Make checks or money orders payable to: COBAALAS

Mail completed form with payment to: Jennifer Quick-Kerns, Battelle Memorial Institute, 505 King Ave., JM-7-110,Columbus, OH 43201. Email: [email protected]. Phone: 614.424.5468. Fa•: 614.458.5468.

Join Us at the Welcome Party!

When: Wednesday, April 30

Time: 6:00 PM – 10:30 PM

Where: Cherry Valley Lodge - Blossom Rooms

A casino nightfilled with fun, food, and prizes

is planned!

th


D5 Poker RunWalk...run...stretch your legs and your brain

on this fun outing!

Follow the scenic trailand try to get the best poker hand as you go.

Stop at stations along the waywhere you will be asked AALAS questions

in order to get your next card.

Prizes are also available for cards.At the end of the trail,

cards will be collected and talliedand the winner will be announced at the final banquet.

WILD

April 30, 2008 1:30 pm

Cost: $5

Additional poker hands: $5 (limit 1)

Additional cards $1

♠

♠

♠

Come out and join us for the fun!

Bet Your Life

on Research2008 District 5 MeetingApril 30-May 2, 2008

Hosted by Central Ohio Branch AALAS

Housing Information and Directions

Cherry Valley Lodge Room Rates

*Make hotel reservations directly with Cherry Valley Lodge*

Room Rates held until April 4 -

2299 Cherry Valley Road $129*/nightNewark, OH 43055 *If you stay for the weekend740.788.1200/800.788.8008 the same rate will apply.

th

From North Columbus

From Downtown Columbus

From Port Columbus International Airport

From Cleveland

From Zanesville

From Cincinnati

From Athens

Take SR 161 East to Granville. SR 161 turns into SR 37. Take SR 37 East to SR 16. Stay on SR 16.Turn right (South) at Cherry Valley Road stoplight. The Cherry Valley Lodge is located ¼ mile south ofSR 16 on the left side.

Take I-70 East to Granville/Lancaster SR 37 Exit. Follow SR 37 North towards Granville. Exit East on SR 16,continue 2 miles and turn right (South) at the Cherry Valley Road stoplight. The Cherry Valley Lodge islocated ¼ mile south of SR 16 on the left side.

From the International Gateway, take I-670 towards I-270. Exit I-270 North, following the I-270 outerbelt toRte. 161 East (New Albany) exit. Travel approximately 20 miles and 161 turns into SR 16. Continue travelingon SR 16 for approximately 3 miles and turn right (South) on to Cherry Valley Road at the stoplight. TheCherry Valley Lodge is located ¼ mile south of SR 16 on the left side.

Take I-71 south to SR 13 south. Take SR 13 south approximately 50 miles to SR 16 west on Newark. Take SR16 west approximately 4 miles to the Cherry Valley Road stoplight and turn left. The Cherry Valley Lodge islocated ¼ mile south of SR 16 on the left side.

Take I-70 West to Granville/Lancaster SR 37 Exit. Follow SR 37 North towards Granville. Exit East on SR 16.Continue 2 miles and turn right (South) at the Cherry Valley Road stoplight. The Cherry Valley Lodge islocated ¼ mile south of SR 16 on the left side.

Take I-71 North to I-270 North (East) to I-70 East. Take I-70 to Granville/Lancaster SR 37 Exit. Follow SR 37North towards Granville. Exit East on SR 16. Continue 2 miles and turn right (South) at the Cherry ValleyRoad stoplight. The Cherry Valley Lodge is located ¼ mile south of SR 16 on the left side.

Take SR 33 West to Lancaster and turn right (North) at the Main St. stoplight; go 1 block and turn left (West) atSR 37 stoplight. Follow SR 37 North towards Granville. Exit East on SR 16, continue 2 miles and turn right(South) at the Cherry Valley Road stoplight. The Cherry Valley Lodge is located ¼ mile south of SR 16 on theleft side.

♠ No Shuttle ProvidedCherry Valley Lodge located approximately 25 miles

From Port Columbus Airport

♠

www.cherryvalleylodge.com

Book Now!

Page 24 District 5 - 2008 COBAALAS

Documents

2008 D5 Program