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Supplemental Figure 1 DGGE band patterns of methanogenic archaeal 16S rRNA genes retrieved from duplicate DNA extractions from two replicate plots in the E and 114 fields at seven time sampling points in 2008, 2009 and 2011. a, E field (BF/BS and AF) in 2008 and 2009; b, 114 field (BF/BS and AF) in 2008; c, 114 field (BF/BS and AF) in 2009; d, E and 114 fields (CG) in 2008; e, E and 114 fields (CG) in 2009; f, E and 114 fields (CG) in 2011. BF/BS, before flooding of paddy rice or before seeding of soybean; CG, crop (rice/soybean) growing period; AF, after

248 2014 477 MOESM1 ESM

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Page 1: 248 2014 477 MOESM1 ESM

Supplemental Figure 1 DGGE band patterns of methanogenic archaeal 16S rRNA genes retrieved from duplicate DNA extractions from two replicate plots in the E and 114 fields at seven time sampling points in 2008, 2009 and 2011. a, E field (BF/BS and AF) in 2008 and 2009; b, 114 field (BF/BS and AF) in 2008; c, 114 field (BF/BS and AF) in 2009; d, E and 114 fields (CG) in 2008; e, E and 114 fields (CG) in 2009; f, E and 114 fields (CG) in 2011. BF/BS, before flooding of paddy rice or before seeding of soybean; CG, crop (rice/soybean) growing period; AF, after crop (rice/soybean) harvest. C, control plot; R, rotational plot; SR, short-term rotational plot; LR, long-term rotational plot.