27 Annual Meeting of the National Laboratories for Avian

Россельхознадзор, ФГБУ «ВНИИЗЖ»

Слайды инженерной службы част ь 2.pptx

27thAnnual Meeting of the National Laboratories for Avian Influenza and Newcastle Disease of European Union

Padova, 6-7 October 2021Update on Avian Influenza situation in the Russian

Federation

Victor N. Irza

Federal Governmental

State-Financed Institution

Federal Centre for Animal Health

FGBI ARRIAH


HPAI epizootic situation in Russia in 2020

• In 2020 (August – December) HPAI was registered in 14 subjects of Federation (83 outbreaks in total)

• Starting in the Urals, epizootics quickly spread to neighboring regions and then to European part of the country

• HPAI cases were reported mostly in backyard and open-type farming poultry (ducks, geese, chickens, turkeys)

• 6 commercial farms were affected: Omsk (1,5 mln layers); Kostroma (0,3 mln layers); Rostov (1,1 mln layers); Astrakhan (0,8 mln layers); Karachaevo-Cherkessiya (0,17mln duck breeders); Tatarstan (0,175 mln fattening turkeys)

• H5N8 and H5N5 detected/isolated in domestic and wild birds

2

Россельхознадзор, ФГБУ «ВНИИЗЖ» 3


PCR positive tests (ARRIAH, 2020)

Poultry & domestic birds

Fed. Okrug/Regions AIV subtype

Ural

Chelyabinsk region H5N8, H9N2

Tumen region H5N8

Kurgan region H5N8

Khanty-Mansi A O – Yugra H5N8

Siberian

Omsk region

H5N8, H5N5

Tomsk region H5N8

South

Astrakhan region H5N8

Krasnodar krai H5N8

Rostov region H5N8

Volgograd region H9N2

North Caucasian

Karachay-Cherkess Rep.

H5N8

Fed. Okrug/Regions AIV

subtype

Volga

Saratov region H5N8

Tatarstan Republic H5N8

Samara region H5N8

Penza region H9N2

Central

Kostroma region H5N8



Wild birds

Fed. Okrug/Regions Bird species AIV subtype

Ural

Chelyabinsk region wild duck H5N8

Tumen region gadwall, garganey, common

pochard, swan, mallard

gadwall

H5N8

H6

Kurgan region tufted duck

coot

H5N8

A

Siberian

Omsk region wild duck H5N8

Buryatia Rep. mallard A

Tomsk region wild waterfowl A

South

Crymea Rep. mute swan H5N8

Rostov region swan H5N8, H5N5

North Caucasian

North Ossetia – Alania swan H5N8

Far Eastern

Amur region wild waterfowl A


A/chicken/Rostov/1836-1/2020(N8) A/chicken/Samara/1938-1/2020(N8) A/wild duck/Chelyabinsk/1207-2/2020(N8) A/chicken/KMAR-Yugra/1925-1/2020(N8)

A/goose/Tatarstan/1823-2/2020(N8) A/chicken/Stavropol/2190-1/20(N8) A/goose/Kurgan/1345-24/2020(N8) A/chicken/Tomsk/1778-7/20(N8)

A/chicken/Uzbekistan/2080-2/20(N8) A/chicken/Kostroma/1761-20/20(N8) A/chicken/Astrakhan/2171-1/20(N8)

A/turkey/Omsk/1312-4/2020(N8) A/duck/Saratov/1578-2/20(N8) A/Duck/Egypt/SMG4/2019/N8 A/swan/Tumen/1479-3/2020(N8)

A/chicken/Rostov/3163/2017(N8) A/chicken/Voronezh/2944/2018(N8)

A/swan/Kaliningrad/85/2017(N8) A/chicken/Tatarstan/804/2017(N8) A/chicken/Moscow/338/2017(N8)

A/duck/KChR/1590-14/20(N8) A/goose/Tatarstan/1730-2/2020(N8) A/duck/Ulyanovsk/1729-1/2020(N8)

A/swan/Krasnodar/43/2017(N5) A/duck/East China/S1109/2014(N8) A/grebe/Tyva/446/2016(N8)

A/chicken/Kostroma/3175/2017(N2) A/goose/Zhejiang/925106/2014(N6)

A/broiler duck/Korea/Buan2/2014(N8) A/wigeon/Sakha/1/2014(N8)

A/duck/Guangdong/wy19/2008(N5) A/duck/Vietnam/LBM638/2014

A/chicken/Jiangxi/10129/2014(N6) A/duck/Vietnam/50/2007

A/Sichuan/1/2006 A/chicken/Hunan/1/2009

A/chick/Bangladesh/14VIR2665-21/14 A/tiger/Jiangsu/01/2013 A/duck/Altai/469/2014

A/pelican/Astrakhan/272/2015 A/duck/Vietnam/NCVD-KA415/2012(N2) A/duck/Tanah Laut/0514095/2014

A/grebe/Tyva/433/2010 A/chicken/Primorsky/85/2008

A/wild duck/Fujian/1/2011 A/duck/Vietnam/LBM132/2012

A/grey heron/Hong Kong/837/2004 A/Bangladesh/3233/2011

A/chicken/Egypt/1071g/2010 A/Egypt/N0001/2015

A/turkey/Turkey/1/2005 A/duck/Novosibirsk/02/05

A/grebe/Tyva/805/06 A/chicken/Krasnodar/776/07

A/Goose/Guangdong/1/1996 A/turkey/Ireland/1378/1983(H5N8)

0.02

I

II

2.3.4.4

Phylogeny of HPAI/H5Nx Russian isolates (2020) • H5N8 and H5N5 HPAIV isolates

belong to clade 2.3.4.4b of Middle

East origin virus introduced to

Russia in 2020

•A few viruses detected in 2020 are

genetically similar to H5N8 viruses

isolated in 2016-2019 in a number of

regions of the Russian Federation

that indicates a local circulation of

the agent in some regions



HPAI epizootic situation in Russia in 2021

• In 2021 (January - September) HPAI was registered in 7 subjects of Federation (17 cases in total as of 17th September)

• In January-February 2021 two outbreaks (H5N8) were notified in commercial poultry farms (Krasnodar – 0,4 mln layers, Rostov – 0,24 mln turkeys)

• In March HPAI cases were reported in wild birds in Dagestan and Astrakhan (H5N5)

• In May HPAIVs detections were reported in wild birds in Tyva (H5N8)

• In June-July HPAI cases were notified in wild birds and domestic birds in backyards in Tyumen Oblast (H5N1)

• In August –September HPAI (H5N1) outbreaks have been reported in Tyumen Oblast and Chelyabinsk Oblast in wild and domestic birds including open-type farming poultry (17000 geese)

• The situation seriously worsened by October:

- mass wild waterfowl die-off in Crimea (H5N5), predominantly Podiceps nigricollis(Black-necked Grebes);

- HPAI cases are suspected in Saratov, Samara, Orenburg regions, samples have been submitted to ARRIAH for confirmation and following notification to OIE

8



Poultry&domestic birds Wild birds

Fed. Okrug/Regions Bird species AIV subtype

South

Krasnodar krai

common heron H5N8

Astrakhan region dalmatian pelican H5N5

North Caucasian

Dagestan Rep.

pelican, gull H5N5

Siberian

Republic of Tyva

dabchick

common pochard, black-

headed gull, tufted duck,

cormorant, great-crested

grebe

black-headed gull

H5N8

H5

H16N3

Ural

Tumen region

dalmatian pelican H5N1

Chelyabinsk region teal, mute swan H5N1

Far Eastern

Rep. of Sakha-Yakutia

common teal H6

Central

Vladimir region

mallard H3, H4N6,

H9N2

Fed. Okrug/Regions AIV

subtype

South

Krasnodar krai H5N8

Rostov region H5N8

Ural

Tumen region H5N1

Chelyabinsk region H5N1

Volga

Samara region H9N2


Wild Birds –HPAI cases notified to OIE in 2021

• H5N8 - Krasnodar Territory - Ardea cinerea (Grey heron);

• H5N5 - Republic of Dagestan – Pelecanus crispus (Dalmatian pelican) and Chroicocephalus (Black-headed gull);

• H5N5 - Astrakhan Oblast – Dalmatian pelican;

• H5N8 - Republic of Tyva - Tachybaptus ruficollis (Dabchick);

• H5N1 - Tyumen Oblast – Dalmatian pelican;

• H5N1 - Chelyabinsk Oblast – Mute Swan, Teal

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https://ru.wikipedia.org/wiki/Серая цапля; //en.wikipedia.org/wiki/Dalmatian_pelicanibundus; https://en.wikipedia.org/wiki/Black-

headed_gull#/media/File:Chroicocephalus_ridibundus_(summer).jpg


Bird species sampled in Tyva in May 2021

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Beloe lake, Piy-Khem district, 9 birds

• Сommon pochard (Aythya ferina) – 2, pos. A/H5

• Black-backed Gull (Larus ridibundus) -1, pos. A/H5

• Tufted Duck (Aythya fuligula) – 2 (1 pos. A/H5 , 1 - type A)

• European сoot (Fulica atra )- 1, AIV- negative

• Little grebe (Podiceps ruficollis) - 3, pos. A/H5N8

Uvs-Nuur lake, Ovyur district, 33 birds

• Great Cormorant (Phalacrocorax carbo) -2, pos. A/H5

• Grey Goose (Anser anser) - 2 (1 – pos. type A, 1 –neg.)

• Great Crested Grebe (Podiceps cristatus) – 10 (4 –pos. type A, 2- pos. A/H5, 4 –neg.)

• Northern Pintail (Anas acuta )- 1, AIV- negative

• Gadwall (Anas strepera) -1, AIV- negative

• Herring gull (Larus argentatus) – 2, pos. type A

• Black-backed Gull (Larus ridibundus) – 15 (5 –pos. type A, 3- pos. A/H5, 7 –neg.)


Phylogeny HPAI/H5 Subtype H5NXRussian isolates

(2021)

A/common heron/Krasnodar/23-1/2021(N8) A/goose/Chelyabinsk/1260-1/2021(N1)

A/pelican/Astrakhan/417-1/2021(N5) A/wild duck/Chelyabinsk/1207-2/20(N8) A/pelican/Dagestan/397-1/2021(N5)

A/dabchick/Tyva/767-51/2021(N8) A/Mute Swan/China/Shandong1/2021(N8)

A/goose/China/21FU006/2020(N8) A/pelican/Tumen/932-1/2021(N1)

A/swan/Rostov/2299-2/2020(N5) A/Duck/Egypt/SMG4/2019/N8

A/chicken/Voronezh/2944/2018(N8) A/chicken/Rostov/3163/2017(N8)

A/swan/Kaliningrad/85/2017(N8) A/duck/KChR/1590-20/20(N8) A/goose/Tatarstan/1730-2/2020(N8)

A/swan/Krasnodar/43/2017(N5) A/duck/East China/S1109/2014(N8) A/grebe/Tyva/446/2016(N8)

A/chicken/Kostroma/3175/2017(N2) A/goose/Zhejiang/925106/2014(N6)

A/wigeon/Sakha/1/2014(N8) A/broiler duck/Korea/Buan2/2014(N8)

A/duck/Vietnam/LBM638/2014 A/chicken/Jiangxi/10129/2014(N6)

A/duck/Guangdong/wy19/2008(N5) A/Sichuan/1/2006

A/chicken/Hunan/1/2009 A/duck/Vietnam/50/2007

A/grey heron/Hong Kong/837/2004 A/chick/Bangladesh/14VIR2665-21/14

A/pelican/Astrakhan/272/2015 A/duck/Altai/469/2014 A/tiger/Jiangsu/01/2013

A/duck/Vietnam/NCVD-KA415/2012(N2) A/duck/Tanah Laut/0514095/2014

A/grebe/Tyva/433/2010 A/chicken/Primorsky/85/2008

A/wild duck/Fujian/1/2011 A/duck/Vietnam/LBM132/2012

A/Bangladesh/3233/2011 A/chicken/Egypt/1071g/2010

A/Egypt/N0001/2015 A/turkey/Turkey/1/2005

A/duck/Novosibirsk/02/05 A/grebe/Tyva/805/06

A/chicken/Krasnodar/776/07 A/Goose/Guangdong/1/1996

A/turkey/Ireland/1378/1983(H5N8)

0.02

2.3.4.4

2.3.4

2.3.2

2.2


A/chicken/Samara/429-75/2021

A/chicken/Volgograd/1290-5/2020

A/chicken/Penza/1890-6/2020

A/chicken/Chelyabinsk/314-1/2020

A/turkey/Israel/1608/2006

A/chicken/Chelyabinsk/30/2019

A/chicken/Zabaikal/2137/2019

A/chicken/Israel/301929/2018

A/chicken/Egypt/D7099/2013

A/quail/Lebanon/273/2010




A/chicken/Pakistan/UVAS26/2019

A/chicken/Bangladesh/40618/2019



A/chicken/Iran/772/1999


A/duck/Guangxi/LAD9/2009(N8)

A/Quail/Hong Kong/G1/97

A/chicken/China/1101/2019

A/chicken/Shandong/1845/2019

A/chicken/Ganzhou/GZ140/2016

A/chicken/Primorsk/419/2018

A/gallus/China/Hefei/2017

A/chicken/Shandong/1757/2019

A/duck/Vietnam/HU1-178/2014(N6)

A/pigeon/Amur/22/2012

A/chicken/Shanxi/55/2011

A/Duck/Hong Kong/Y280/97

AY743216 Shanghai/F/98

A/Anas plat./Belgium/9074/2016(N2)

A/teal/Primorie/3631/02(N2)

A/pelican/Zambia/13/2009(N1)

A/Duck/Hong Kong/Y439/97

A/duck/Vietnam/OIE-2334/2010(N6)

A/duck/HK/147/1977(N6)

0.02

G1

Y280

Y439

Phylogeny H9

Subtype H9N2

Russian isolates (2020-2021)



Conclusions

• HPAIVs H5N8, H5N5, H5N1 of clade 2.3.4.4.b are currently circulating in the Russian Federation

• Further reassortment events are possible

• The scenario of the spread of the disease is the same as last year (2020)

• LPAI H9N2 continues to threaten commercial poultry

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Thank you for

your attention!

600901 Russia, Vladimir, UrievetsTel/Fax: +7 (4922) 26-38-77,mobile: +7 910 774 01 99E-mail: [email protected]@arriah.ru

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27 Annual Meeting of the National Laboratories for Avian