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Appendix Table 1. Influenza A virus (H5N1) detection in environmental specimens collected in 3 villages of Kampong Cham and Prey Veng provinces, Cambodia, February–August 2006 Environmental specimens Viral load, copies Specimen source Wet or dry specimen Out or in household yards Total No. (%) positive* Mean Maximum Minimum Wet feces Wet In 4 2 (50) 2,300 2,300 2,300 Water Pond water Wet In 1 0 Poultry ranging place in wetlands Wet Out 3 1 (33) 16,000 16,000 Pond water plants Wet In 2 1 (50) 16,000 16,000 Mud Poultry ranging place in wetlands Wet Out 7 1 (14) 510,000 510,000 Mud beneath animal cages (poultry, pigs) Wet In 4 2 (50) 880 1,000 760 Mud by the lake shore Wet Out 1 1 (100) 45,000 45,000 Mud from ponds Wet In 19 4 (21) 5,150 8,700 1,600 Mud in household's yard Wet In 3 1 (33) 1,700 1,700 Dry soil in household yard Dry In 1 1 (100) 1,800 1,800 Soil swab Beneath houses on stilts Dry In 7 3 (43) 4,350 7,300 1,400 Poultry places Dry In 7 5 (71) 10,500 20,000 1,000 Pig places Dry In 4 0 Poultry ranging place in wetlands Wet Out 4 2 (50) 7,700 8,000 7,400 Surface swab Feathers of dead poultry Dry In 6 3 (50) 48,000 85,000 11,000 Chicken and pig cages Dry In 2 0 House wall Dry In 1 0 Rice straw stock Dry In 1 0 Total 77 27 *Specimens tested positive by reverse transcription–PCR; of these, no virus was isolated.

4 2 (50) 2,300Poultry ranging place in wetlands Wet Out 7 1 (14) 510,000 510,000 Mud beneath animal cages (poultry, pigs) Wet In 4 2 (50) 880 1,000 760 Mud by the lake shore Wet Out

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Page 1: 4 2 (50) 2,300Poultry ranging place in wetlands Wet Out 7 1 (14) 510,000 510,000 Mud beneath animal cages (poultry, pigs) Wet In 4 2 (50) 880 1,000 760 Mud by the lake shore Wet Out

Appendix Table 1. Influenza A virus (H5N1) detection in environmental specimens collected in 3 villages of Kampong Cham and Prey Veng provinces, Cambodia, February–August 2006

Environmental specimens Viral load, copies Specimen source

Wet or dry specimen

Out or in household yards Total No. (%) positive* Mean Maximum Minimum

Wet feces Wet In 4 2 (50) 2,300 2,300 2,300 Water Pond water Wet In 1 0 Poultry ranging place in wetlands Wet Out 3 1 (33) 16,000 16,000 Pond water plants Wet In 2 1 (50) 16,000 16,000 Mud Poultry ranging place in wetlands Wet Out 7 1 (14) 510,000 510,000 Mud beneath animal cages (poultry, pigs) Wet In 4 2 (50) 880 1,000 760 Mud by the lake shore Wet Out 1 1 (100) 45,000 45,000 Mud from ponds Wet In 19 4 (21) 5,150 8,700 1,600 Mud in household's yard Wet In 3 1 (33) 1,700 1,700 Dry soil in household yard Dry In 1 1 (100) 1,800 1,800 Soil swab Beneath houses on stilts Dry In 7 3 (43) 4,350 7,300 1,400 Poultry places Dry In 7 5 (71) 10,500 20,000 1,000 Pig places Dry In 4 0 Poultry ranging place in wetlands Wet Out 4 2 (50) 7,700 8,000 7,400 Surface swab Feathers of dead poultry Dry In 6 3 (50) 48,000 85,000 11,000 Chicken and pig cages Dry In 2 0 House wall Dry In 1 0 Rice straw stock Dry In 1 0 Total 77 27 *Specimens tested positive by reverse transcription–PCR; of these, no virus was isolated.

Page 2: 4 2 (50) 2,300Poultry ranging place in wetlands Wet Out 7 1 (14) 510,000 510,000 Mud beneath animal cages (poultry, pigs) Wet In 4 2 (50) 880 1,000 760 Mud by the lake shore Wet Out

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