PATENT ABSTRACTS

4956296

C L O N E D S T R E P T O C O C C A L G E N E S E N C O D I N G P R O T E I N G

A N D T H E I R U S E T O C O N S T R U C T R E C O M B I N A N T

M I C R O O R G A N I S M S T O P R O D U C E P R O T E I N G

Stephen R Fahnestock assigned to Genex Cor- poration

A cloned gene encoding Protein G, or func- tionally active portions thereof, vectors con- raining the cloned gene, and microorganisms transformed by those vectors are disclosed.

4956455

B O V I N E F I B R O B L A S T G R O W T H F A C T O R

Andre Baird, Frederick S Esch, Peter Bohlen, Deni Gospodarowicz, Nicholas C Ling assigned to The Salk Institute for Biological Studies

Substantially pure bovine pituitary fibroblast growth factor, a 146 amino acid residue poly- peptide, is produced. The amino acid residue sequence of bpFGF is disclosed as well as a DNA chain encoding the polypeptide. By ap- propriately inserting a synthesized DNA chain into a cloning vector and using the cloning vector to transform cells, synthetic bpFGF can be ob- tained from transformed cell lines, both prokaryotic and eukaryotic.

4957739

P H A R M A C E U T I C A L C O M P O S I T I O N S O F A 105 K D P .

H A E M O L Y T I C A D E R I V E D A N T I G E N U S E F U L F O R

T R E A T M E N T O F S H I P P I N G F E V E R

Peter Berget, Michael Engler, Sarah Highlander, George Weinstock assigned to Board of Regents The University of Texas System

Novel compositions are disclosed for use in the treatment or diagnosis of bovine pasteurellosis, commonly referred to as Shipping Fever. Cell- free Pasteurella haemolytica supernatants are employed to provide individual antigen com- positions, identified through reaction with sera

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from naturally-infected or convalescent cattle. In particular, at least seven individual P. hameolytica antigen groups were recognized in cell-free culture supernatants. Purified P. haemolytiea supernatant, formulated in a suitable pharmaceutical vaccine composition is shown to elicit a specific immune response, in both cows and rabbits, directed against the in- dividual immunoreactive P. haemolytica poly- peptides identified. Also disclosed are novel recombinant cells, plasmids and bacteriophage which include transcriptionally active P. haemolytica antigen genes. Recombinant clones are similarly selected to be reactive with naturally-infected antisera. Examples, and fur- ther disclosure, are also provided which demons- trate the utility of a presently disclosed antibody and antigen compositions in immunodetection of both antigens and antibodies in various bio- logical samples.

4957858

R E P L I C A T I V E R N A R E P O R T E R S Y S T E M S

Barbara Chu, Fred R Kramer, Pau Lizardi, Les- lie Orgel assigned to The Salk Instute for Bio- logical Studies; The Trustees of Columbia Universi

Highly sensitive methods for assaying for bio- polymers, such as by immunoassay or nucleic acid probe hybridization assay, and composi- tions for carrying out the methods, are provided. The methods employ as reporter group a RNA capable of being autocatalytically replicated by an RNA-dependent RNA polymerase, such as the replicase of bacteriophage Q beta. The high sensitivity of the assay methods is due to the rapid, exponential increase in concentration of such a replicative RNA, associated specifically with biopolymer analyte, that can be achieved by autocatalytic RNA replication.

4957861

P R O C E S S F O R T H E B I O T E C H N O L O G I C A L

P R E P A R A T I O N O F P O L Y D ( - ) 3 - H Y D R O X Y B U T Y R I C A C I D

Robert M Lafferty, Gerhar Braunegg, Graz, Austria assigned to Petrochemie Danubia Ges m b H

The present invention relates to a process for the biotechnological preparation of poly-D-(-)-3-