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4994389

PATENT ABSTRACTS

P R O M O T E R - P R O B E P L A S M I D F O R A N A L Y S I S O F

T R A N S C R I P T I O N A L R E G U L A T I O N

Jerald Feitelson assigned to American Cyanamid Company

The invention provides a recombinant plasmid that is capable of inducing the expression of a brown pigment. The plasmid is a pARCI plasmid into which has been inserted DNA which in- cludes a transcriptional terminator and a poly- linker sequence.

4994565

N U C L E O T I D E S E Q U E N C E O F G E N E S P E C I F I C A L L Y

E X P R E S S E D BY I N S U L I N O M A

Hiroshi Okamoto, Sendai, Japan assigned to Tohoku University

From a cDNA library, a nucleotide sequence of a novel gene, called rig, specifically expressed by streptozotocin- or alloxan-nicotinamide- induced rat insulinomas was determined. Fur- ther, novel genes with base sequences homologous to rig were found in a BK virus- induced hamster insulinoma and in a surgically removed human insulinoma. The above DNA is transcribed to provide an mRNA. The above DNAs and mRNAs can be efficaciously em- ployed for the medical purposes of pancreatic diseases.

4996143

F L U O R E S C E N T S T O K E S S H I F T P R O B E S F O R P O L Y N U C L E O T I D E

H Y B R I D I Z A T I O N

Michael Heller, Edward J Jablonski assigned to Syngene Inc

Fluorescent stokes shift probes for poly- nucleotide hybridization assays are designed to provide predetermined nucleotide base unit spacings between the donor and acceptor fluorophores. When the probes are hybridized to the target polynucleotide the fluorophores paired for non-radiative energy transfer are separated by 2 to 7 nucleotide base units. The

fluorophores are attached to the DNA or RNA probes by linker arms having lengths of 4 to 30 Angstroms. Fluorescein is a preferred donor for use with a Texas Red acceptor.

4996144

M I C R O A S S A Y F O R D E T E C T I O N O F D N A A N D R N A

Anne Crossway, Catherine M Houck assigned to Calgene lnc

A novel hybridization diagnostic technique is in- volved employing micromanipulation of cells for detection of DNA or RNA in the cells. Cellular suspensions are employed, the DNA or RNA purified by microextraction using liquid media having differential solubilization properties, whereby the nucleic acid is retained in an aqueous microdrop. The nucleic acid in the microdrop may then be assayed with a nucleic acid probe in accordance with conventional ways.

4996148

A80407 A N T I B I O T I C S

Lawrence E Doolin, Richard M Gale, Otis W Godfrey, Robert Hamill, David Mahoney, Raymond C Yao assigned to Eli Lilly and Com- pany

New glycopeptide antibiotic A80407, com- prising A80407A and A80407B, is produced by Kibdelosporangium philippinensis strains NRRL 18198, and NRRL 18199 or AS0407- producing mutants of these strains. The A80407 antibiotics have excellent activity against Gram- positive bacteria, increase feed-utilization effi- ciency in monogastric and ruminant animals and improve weight gains in animals.

4996151

M E T H O D F O R P R O D U C I N G T H E E A G I R E S T R I C T I O N

E N D O N U C L E A S E A N D M E T H Y L A S E

Joan E Brooks, Laura Sznyter assigned to New England Biolabs Inc

The present invention is directed to a method for cloning and producing the Eag 1 restriction

PATENT ABSTRACTS

endonuclease by (1) introducing the restriction endonuclease gene from E. agglomerans ATCC into a host whereby the restriction gene is ex- pressed; (2) fermenting the host which contains the vector encoding and expressing the Eag I restriction endonuclease, and (3) purifying the Eag I restriction endonuclease from the fermen- ted host which contains the vector encoding and expressing the Eag I restriction endonuelease ac- tivity.

4996152

A V I A N H E R P E S V I R U S A M P L I C O N A S A E U C A R Y O T I C

E X P R E S S I O N V E C T O R

Jeanne Carter, Robert Silva assigned to The United States of America as represented by the Secretary of Agriculture

DNA fragments (seeds) having the charac- teristics of amplicons, which are useful for am- plifying genes of interest, have been isolated from Marek's disease viruses of poultry. Con- catmers of the seeds and the associated genes have potential as vaccines or delivery vectors when cotransfected and replicated in the pre- sence of helper viruses. The amplicons are also useful for inserting associated genes into the hel- per viruses, which in turn could be used as ex- pression vectors. Candidate genes for use with the subject amplicons include those which encode immunogenic proteins and other benefi- cial economic traits desired in commercial poultry lines.

4996155

B A C I L L U S T H U R I N G I E N S I S G E N E E N C O D I N G A

C O L E O P T E R A N - A C T I V E T O X I N

August Sick, Thomas E Gilroy assigned to Mycogen Corporation

A novel B.t. toxin gene encoding a protein toxic to coleopteran insects has been cloned from a novel coleopteran-active B. thuringiensis microbe. The DNA encoding the B.t. toxin can

279

be used to transform various prokaryotic and eukaryotic microbes to express the B.t. toxin. These recombinant microbes can be used to con- trol coleopteran insects in various environments.

4996156

P R E P A R A T I O N O F S P O R E - F R E E , C O N C E N T R A T E D P R O T E I N

P R E P A R A T I O N S F R O M B A C I L L U S T H U R I N G I E N S I S

S E R O V A R , I S R A E L E N S I S , W H I C H I S T O X I C F O R G N A T S , A

M I C R O O R G A N I S M F O R T H E I R P R E P A R A T I O N , A N D T H E

I S O L A T I O N O F T H E M I C R O O R G A N I S M

Han Zaehner, Konrad Bernhard, Harald Weis- ser, 7400 Tuebingen, Federal Republic Of Ger- many

Asporous mutants of Bacillus thuringiensis serovar, israelensis, a process for their prepara- tion and their use for the isolation of bacterial toxins for controlling diptera.

4996390

N O V E L I N T E R S P E C I F I C M U S H R O O M S T R A I N S

Kurt R Dahlberg assigned to Campbell Soup Company

This invention provides strains of commercial mushrooms whose genetic material comprises genes from more than one species. These strains include a novel strain of the commercial mushroom genus Agaricus which is a cellular hybrid produced by fusion between protoplasts of two different Agaricus species. In another em- bodiment, this invention provides improved strains for commercial production of mushroom fruit which are progeny from crosses of Agaricus sp. with a cellular hybrid produced by fusion bet- ween protoplasts of two different species of the genus Agaricus.