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6X DNA Loading Dye
6X Orange DNA Loading DyeDescription6X Orange Loading Dye is used to prepare DNA markers and samples for loading on agarose or polyacrylamide gels. It contains two different dyes (xylene cyanol FF and orange G) for visual tracking of DNA migration during electrophoresis. The presence of glycerol ensures that the DNA in the ladder and sample forms a layer at the bottom of the well. The EDTA included in the solutions binds divalent metal ions and inhibits metal-dependent nucleases.6X Orange DNA Loading Dye is used for conventional DNA electrophoresis (seerecommendations for use).
Quality ControlTested for DNA sample preparation prior to agarose gel electrophoresis. The absence of deoxyribonucleases confirmed by appropriate tests.
Composition
10 mM Tris-HCl (pH 7.6),
0.15% orange G,
0.03% xylene cyanol FF,
60% glycerol,
60 mM EDTA
StorageStore at room temperature or at 4°C up to 12 months.For longer periods, store at -20°C.
6X Bromophenol blue/Xylene cyanol DNA Loading Dye
Description6X DNA Loading Dye is used to prepare DNA markers and samples for loading on agarose or polyacrylamide gels. It contains two different dyes (bromophenol blue and xylene cyanol FF) for visual tracking of DNA migration during electrophoresis. The presence of glycerol ensures that the DNA in the ladder and sample forms a layer at the bottom of the well. The EDTA included in the solution binds divalent metal ions and inhibits metal-dependent nucleases.6X DNA Loading Dye is used for conventional DNA electrophoresis (see recommendations for use).
Quality ControlTested for DNA sample preparation prior to agarose gel electrophoresis. The absence of deoxyribonucleases confirmed by appropriate tests.
Composition
10 mM Tris-HCl (pH 7.6),
0.03% bromophenol blue,
0.03% xylene cyanol FF,
60% glycerol,
60 mM EDTA
StorageStore at room temperature or at 4°C up to 12 months.For longer periods, store at -20°C.
Loading Buffer with GelRed for Electrophoresis
To do this buffer you can use either Bromophenol blue or Xylene Cyanol buffer described above. This protocol will make 5ml
of 6x solution. Adjust to your needs if necessary.
1. 5 ml Loading Buffer (Xylene-cyanol or Bromophenol Blue)