7. Penyebab Virus - Rhabdiviridae (RABIES) & RotaVirus

7/21/2019 7. Penyebab Virus - Rhabdiviridae (RABIES) & RotaVirus

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RHABDOVIRUSRHABDOVIRUS

Disease : RabiesDisease : Rabies CNS inflammationCNS inflammation

Spread by infected animal-bite.Spread by infected animal-bite.

Viral characteristicViral characteristic

A.A. Virus structure :Virus structure :

•Rhabdovirus : rode /roundRhabdovirus : rode /round∅

! " #$% nm.! " #$% nm.•&lycoproteins cover p ' #% nm.&lycoproteins cover p ' #% nm.

•&enome : sin(le stranded RNA ) *+ , " #%&enome : sin(le stranded RNA ) *+ , " #%

•ave RNA polymeraseave RNA polymerase



B.B. Reaction against physical & chemical substance :Reaction against physical & chemical substance :

Resistant in t !Resistant in t ! ""°

## in se$eral %ees.in se$eral %ees.

Inacti$ation by Inacti$ation by #O#O''..

( : ( : UV) in t *+UV) in t *+°

# an hour# an hour) lipi, sol$ent) lipi, sol$ent

-ether) +)/ So,ium ,eo0ycholic1) trypsin)-ether) +)/ So,ium ,eo0ycholic1) trypsin)

,etergent & e0treme pH,etergent & e0treme pH#. #. Antigen characteristic:Antigen characteristic:

Only serotype but the strain can ,i22erent)Only serotype but the strain can ,i22erent)

base, on : base, on :

Species Species 3eographic 3eographic

4he ,i22erentiation on nucleoprotein & 4he ,i22erentiation on nucleoprotein &

glycoprotein glycoprotein



D. Animal susceptibility & Virus gro%ing :D. Animal susceptibility & Virus gro%ing :

5i,e susceptibility host 5i,e susceptibility host→

all animalall animalpoiilothermic & human coul, be in2ecte,. poiilothermic & human coul, be in2ecte,.

Virus : #6S) sali$a) urine) lymph) mil & bloo,. Virus : #6S) sali$a) urine) lymph) mil & bloo,.

Strain : Strain :

. 7ong incubation perio, strain -'8+ ,ays1. 7ong incubation perio, strain -'8+ ,ays1

inclusion bo,ies in cytoplasminclusion bo,ies in cytoplasm

'. Short incubation perio, strain -"8 ,ays1'. Short incubation perio, strain -"8 ,ays1

hasn9t inclusion bo,ies. hasn9t inclusion bo,ies.





#linical appearance

Rabies primary animal ,iseaseRabies primary animal ,isease human) throughhuman) through

. In2ecte,animal bite. In2ecte,animal bite

'. #ontact %ith in2ecte,sali$ary animal'. #ontact %ith in2ecte,sali$ary animal

Incubation perio, 8 %eesIncubation perio, 8 %ees

4he sign ,i$i,e, into " phases4he si

gn ,i$i,e, into " phases ::

. ro,ormal phase: 2e$er) malaise) anore0ia) hea,ache). ro,ormal phase: 2e$er) malaise) anore0ia) hea,ache)

nausea & $omitingnausea & $omiting

'. Sensory phase : 7acrimation) hallucination) muscle rigi,ity.'. Sensory phase : 7acrimation) hallucination) muscle rigi,ity.

;. <0citement phase : photophobia ) soun,) & %ater.;. <0citement phase : photophobia ) soun,) & %ater.

". aralytic phase : ascen,ing muscle paralytic". aralytic phase : ascen,ing muscle paralytic



7aboratory Diagnosis

A.A. =icroscopic:=icroscopic:

• Immuno2luorescensi : rabies hamster antiseraImmuno2luorescensi : rabies hamster antisera

• Brain or cornea impression.Brain or cornea impression.

• athognomonicathognomonic : negri bo,ies->1 in muscle ?=S: negri bo,ies->1 in muscle ?=SB.B. Virus isolation:Virus isolation:

• In2ecte, tissue -sali$a1In2ecte, tissue -sali$a1 inoculate to mouse intrainoculate to mouse intracerebral.cerebral.

• ->1 : 7eg 2lacci, paraly@e,) encephalytic & ,eath.->1 : 7eg 2lacci, paraly@e,) encephalytic & ,eath.

• #6S9s mouse e0amination#6S9s mouse e0amination 6egri bo,ies & rabies Ag6egri bo,ies & rabies Ag



#. Serology :#. Serology :

Ab test against rabies : I) # or 6t test. Ab test against rabies : I) # or 6t test. Ab : ->1 in human ? animal along sic. Ab : ->1 in human ? animal along sic.

D.D. Animal obser$ation:Animal obser$ation:

. Suspecte, animal rabies. Suspecte, animal rabies ille,ille, tissuetissue

e0amination.

'. Other animal rabies possibility'. Other animal rabies possibility stalle,stalle,

& obser$e, about + ,ays. & obser$e, about + ,ays.

;. I2 sign ->1;. I2 sign ->1 ille,ille, & tissue e0am.& tissue e0am.



Immunity & re$entingImmunity & re$enting

Rabies pre$enting pathophysiology through $accine:Rabies pre$enting pathophysiology through $accine:

. Virus replication in muscle close, 2rom inoculation. Virus replication in muscle close, 2rom inoculation

sitesite $irus$irus

enough to cause in2ection o2 #6S.enough to cause in2ection o2 #6S.

'. 3i$ing $accine ? immunogenic substance promptly'. 3i$ing $accine ? immunogenic substance promptly

,epress $irus replication & prohibit in$asion to #6S. ,epress $irus replication & prohibit in$asion to #6S.

;. Action o2 passi$e ab :;. Action o2 passi$e ab :

a. inoculate, in2ecte, $irus & $irus ,ecreasea. inoculate, in2ecte, $irus & $irus ,ecrease

b. to gi$e enough time to $accine to stimulate acti$e ABb. to gi$e enough time to $accine to stimulate acti$e AB

pro,uction to prohibit $irus entering to #6S. pro,uction to prohibit $irus entering to #6S.



Vaccine type :Vaccine type :

. Human ,iploi, cell $accine-HD#V1. Human ,iploi, cell $accine-HD#V1

'. Rabies $accine absorption -RVA1'. Rabies $accine absorption -RVA1

;. 6eural tissue $accine -64V1;. 6eural tissue $accine -64V1

". Doc embryo $accine -D<V1". Doc embryo $accine -D<V1

*. Attenuate, li$ing $accine*. Attenuate, li$ing $accine



REOVIRIDAE

ROTA VIRUS



6 genus :- Reovirus- Orbivirus

- Rotavirus3 genus pada pertumbuhan

REOVIRIDAE



-en!ebab pen!" sa#" $erna% sa#" na&aspada manusia ' he(an

- Sindroma )#ini) : be#um *e#as

- +udah diiso#asi dari tin*a dari padadari hidung , tenggoro)an

- Tumbuh subur pada bia)an gin*a# )era $pe

Reo . Respirator! Enteri) Orphan

REOVIRIDAE REOVIRUS



/ Orbi . $in$in , #ing)aran

/ ertama)a#i ditemu)an o#eh 0arden d))

121/ 0er)embangbia) da#am : serangga%

Vertebrata% Tumbuhan

/ ada manusia : demam ringan% sa)it)epa#a

/ 4ang pa#ing patogen men!ebab 50#ueton ue #idah biru ada )ambin

REOVIRIDAE OR0IVIRUS



• / 0entu) virus men!erupai roda 7rota . roda8

• / ertama)a#i ditemu)an o#eh 0ishop d)) 123

pd pend" diare non-ba)teria# di Austra#ia• / 0ersi&at invasi&

/ enu#aran . oro-&e$a#

•

/ Terutama men!erang ana)-ana) 6 b# - 9 th• / ama #ain :

– - Duovirus

– - I;V . In&anti#e ;astroenteritis Virus

– - <RV= . <uman Rotavirus =i)e Agent

REOVIRIDAE ROTAVIRUS



/ 0ervariasi : Diare ringan - berat Dehidrasi S!o) >

/ Se#& #imiting disease Sembuh da#ambeberapa hari

/ ?has : Sebe#um diare ter*adi +untah-muntah @ suhu tinggi

;ambaran ?#ini)



• / Re#ati& stabi#

• / Resisten terhadap pe#arut-pe#arut #ipid

• / Susah dibia) pada bia)an *aringan

Si&at RotavirusSi&at Rotavirus



• / +i)ros)op e#e)tron :- maha#

- tenaga te)nisi )husus

- sangat )omp#e)s

• / 0erdasar)an intera)si Antigen-antibodi :- =ate) ag#utinatin test

- Radio Immuni Assa! 7RIA8

- Reverse assive <aemog#utination 7R<A8- o#!a$r!#amide ;e# E#e$trophorese 7A;E8

- u$#ei$ A$id <!bridisation 7A<8

- E#isa 7En!m =in)ed Imuno Sorbent Assa!8

/ Diagnosis $epat

Diagnosis Rotavirus



/ Supporti&/ +en$egah dehidrasimenga)ibat)an s!o)

Terapi RotavirusTerapi Rotavirus

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7. Penyebab Virus - Rhabdiviridae (RABIES) & RotaVirus