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TRYPTIC SOY BROTH with FERROUS SULFATE (7739)
Intended UseTryptic Soy Broth with Ferrous Sulfate is used in
the pre-enrichment of Salmonella for shell and liquidwhole eggs as
described in FDA/Bacteriological Analytical Manual.
1
Product Summary and ExplanationTryptic Soy Brothis a general
purpose medium used for the cultivation of a wide variety of
microorganisms,and conforms to Harmonized USP/EP/JP
Requirements.
2,3,4Tryptic Soy Broth, is commonly referred to as
Soybean-Casein Digest Medium, and abbreviated as TSB. Tryptic
Soy Broth complies with establishedstandards
5,6in the food industry.
Tryptic Soy Broth with Ferrous Sulfate is used in the
FDA/Bacteriological Analytical Manual (BAM) Methodfor the
Identification and Isolation of Salmonella in Eggs.
1TSB with Ferrous Sulfate is used in the Pre-
Enrichment Step for Shell Eggs and Liquid Whole Eggs
(Homogenized). 1 Egg-associated illness caused bySalmonellais a
serious public health problem.
7Salmonella enteritidishas been implicated in egg-associated
illness, and can cause abdominal cramps, fever, and diarrhea
beginning 12 to 72 hours after consuming acontaminated food or
beverage.
7Salmonella enteritidiscan be found inside eggs that appear
normal.
8Eggs
in the shell become contaminated on the farm, primarily because
of an infection in the laying hens.8
Principles of the ProcedureEnzymatic Digest of Casein and
Enzymatic Digest of Soybean Meal are nitrogen sources in Tryptic
SoyBroth with Ferrous Sulfate. Dextrose is the carbon energy source
that facilitates organism growth in thismedium. Sodium Chloride
maintains osmotic balance and Dipotassium Phosphate is a buffering
agent.Ferrous Sulfate is added to assist in the recovery of injured
target Salmonellastrains.
Formula / Liter
Enzymatic Digest of Casein
................................................. 17.0 gEnzymatic
Digest of Soybean Meal ....................................... 3.0
gSodium Chloride
....................................................................
5.0 gDipotassium Phosphate
......................................................... 2.5
gDextrose
.................................................................................
2.5 gFerrous Sulfate
..................................................................
0.035 gFinal pH: 7.3 0.2 at 25CFormula may be adjusted and/or
supplemented as required to meet performance specifications.
Precautions1. For Laboratory Use.2. IRRITANT. Irritating to
eyes, respiratory system, and skin.
Directions1. Suspend 30 g of the medium in one liter of purified
water.2. Autoclave at 121C for 15 minutes.
Quality Control SpecificationsDehydrated Appearance: Powder is
homogeneous, free flowing, and light beige.
Prepared Appearance: Prepared medium is clear to brilliantly
clear, no to light precipitate, and yellow toamber.
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ExpirationRefer to expiration date stamped on the container. The
dehydrated medium should be discarded if not freeflowing, or if the
appearance has changed from the original pale to light beige.
Expiry applies to medium in its
intact container when stored as directed.
Limitation of the ProcedureDue to nutritional variation, some
strains may grow poorly or fail to grow on this medium.
PackagingTryptic Soy Broth with Ferrous Sulfate Code No. 7739A
500 g
7739B 2 kg7739C 10 kg
References1.
http://www.fda.gov/Food/ScienceResearch/LaboratoryMethods/BacteriologicalAnalyticalManual/BAM/default.htm
2. United States Pharmacopeial Convention. 2007. The United States
pharmacopeia, 31
sted., Amended Chapters 61, 62, 111. The
United States Pharmacopeial Convention, Rockville, MD.3.
Directorate for the Quality of Medicines of the Council of
Europe (EDQM).2007.
The European Pharmacopoeia, Amended
Chapters 2.6.12, 2.6.13, 5.1.4, Council of Europe, 67075
Strasbourg Cedex, France.4. Japanese Pharmacopoeia. 2007. Society
of Japanese Pharmacopoeia. Amended Chapters 35.1, 35.2, 7. The
Minister of Health,
Labor, and Welfare.5. Vanderzant, C., and D. F. Splittstoesser
(eds.). 1992. Compendium of methods for the microbiological
examination of foods, 3
rd
ed. American Public Health Association, Washington, D.C.6.
Cunnif, P. 1995. Official methods of analysis AOAC International,
16
thed. AOAC International, Arlington, VA.
7. http://www.cdc.gov/ncidod/dbmd/diseaseinfo/salmet_g.htm 8.
http://www.fda/gov/NewsEvents/newsroom/PressAnnouncements/ucm218461.htm
Technical InformationContact Acumedia Manufacturers, Inc. for
Technical Service or questions involving dehydrated culture media
preparation orperformance at (517)372-9200 or fax us at
(517)372-2006.
http://www.fda.gov/Food/ScienceResearch/LaboratoryMethods/BacteriologicalAnalyticalManual/BAM/default.htmhttp://www.fda.gov/Food/ScienceResearch/LaboratoryMethods/BacteriologicalAnalyticalManual/BAM/default.htmhttp://www.cdc.gov/ncidod/dbmd/diseaseinfo/salmet_g.htmhttp://www.cdc.gov/ncidod/dbmd/diseaseinfo/salmet_g.htmhttp://www.cdc.gov/ncidod/dbmd/diseaseinfo/salmet_g.htmhttp://www.fda.gov/Food/ScienceResearch/LaboratoryMethods/BacteriologicalAnalyticalManual/BAM/default.htm
