8704459

PATENT ABSTRACTS

R E T R O V I R U S O F T H E H I V - 2 T Y P E S U S C E P T I B L E O F

I N D U C I N G A I D S , A N D I T S A N T I G E N I C A N D N U C L E I C

C O N S T I T U E N T S

Luc MONTAGNIER, Solange CHAMARET, Denise GUETARD, Marc ALIZON, Fran@ois CLAVEL, Mireille GUYADER. Pierre SONIGO, Fran@oise BRUN-VEZINET, Marianne REY, Christine ROUZIOUX, Christine KATLAMA, 21, rue de Malabry, F- 92350 Le Plessis Robinson, France assigned to INSTITUT PASTEUR

New variety ofretroviruses, designated by HIV- 2, of which samples have been deposited at ECACC under the numbers 87.01.1001 and 87.01.1002 and at NCIB under the numbers 12.398 and 12.399. The invention also relates to the antigens susceptible of being obtained from said virus, in particular from the proteins pl2, p16, p26 and gp 140. These different antigens are applicable to the diagnosis of the disease, par- ticularly by contact with a serum of the patient on whom the diagnosis is to be carried out. It also relates to immunogenic compositions con- taining more particularly the glycoprotein spl40. Finally, it relates to nucleotidic sequences useable particularly as hybridization probes derived from the RNA of HIV-2.

8704461

T H E R M A L L Y S T A B L E A N D P H S T A B L E S U B T I L I S I N A N A L O G S

A N D M E T H O D F O R P R O D U C T I O N T H E R E O F

Yitzhak STABINSKY, Mark M ZUKOWSKI, Mark M ZUKOWSKI assigned to AMGEN

A mutated subtilisin suitable for admixture to washing compositions and exhibiting substan- tially improved stability over naturally occuring Bacillus serine proteases is prepared by ex- pressing a modified gene encoding subtilisin in Bacillus subtilis). A preferred subtilisin analog product differs from wild-type Bacillus alkaline proteases by having any amino acid, and pre- ferably serine, at position (218) in place of asparagine. The product is preferably produced in a strain of B. subtilis) which is mutated to block synthesis of endogenous proteases. The method of replacing an Asn or Gly in an Asn-

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Gly sequence in order to improve pH and ther- mal stability may be applied to other sites in substilisin and to other proteins as well.

8704462

R E C O M B I N A N T D N A S E Q U E N C E S , V E C T O R S

C O N T A I N I N G T H E M A N D M E T H O D F O R T H E U S E

T H E R E O F

Richard Harris WILSON. Christopher Robert BEBBINGTON, 48 Galbraith Drive. Mil- ngavie, Glasgow G62 6NE, United Kingdom as- signed to CELLTECH LIMITED; THE UNIVERSITY COURT OF THE UNIVER- SITY OF GLASGOW

Recombinant DNA sequences which encode the complete amino acid sequence of a glutamine synthetase, vectors containing such sequences, and methods for their use, in particular as dominant selectable markers, for use in co- amplification of non-selected genes and in trans- forming host cell lines to glutamine independence.

8704463

A T T E N U A T E D H E R P E S V I R U S E S , H E R P E S V I R U S E S W H I C H I N C L U D E F O R E I G N D N A

E N C O D I N G A N A M I N O A C I D S E Q U E N C E A N D V A C C I N E

C O N T A I N I N G S A M E

Mark D COCHRAN, Meng-Fu SHIH. William P MacCONNEL, Richard D MACDONALD assigned to SYNTRO CORPORATION

A hybrid, nonprimate herpesvirus and at- tenuated nonprimate herpesviruses comprising DNA which includes a sequence essential for viral replication at least a portion of which is pre- sent in a sequence essential for replication of a naturally-occurring primate herpesvirus and at least one foreign DNA sequence, which in the at- tenuated viruses is antigenic in the host. These attenuated viruses are useful as vaccines for im- munizing animals against herpesvirus disease. The invention also provides a method for dist- inguishing an animal vaccinated with a vaccine of the present invention from an animal infected with a naturally occurring pseudorabies virus.