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LacZ. wtALK1/HA. - TGF- b. - TGF- b. - TGF- b. +TGF- b. +TGF- b. +TGF- b. MEEC BRE-luc. 120. 100. 80. Luciferase activity (arbitrary unit s ). 60. 40. 20. 0. -. -. ALK1- RNAi. BAEC. A. MEEC BRE-luc. B. 45 min. 45 min. 90 min. 90 min. 180. TGF- b:. 3h. 2h. 2h. 3h. - PowerPoint PPT Presentation
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A.
4h0 45 m
in
90 m
in
2h 3h 4h0 45 m
in
90 m
in
2h 3h
actin
LacZ wtALK1/HA
HA
TGF-
Phospho-Smad1/5
Phospho-Smad2
ALK1RNAi
+hALK1
C.
- siRNA-Smad5
Smad5RNAi
+mSmad5
Luci
fera
se a
ctiv
ity(a
rbitr
ary
units
)
0
10
20
30
40
50
60
70
80
D.
BAEC
B.
0
20
40
60
80
100
120
Luci
fera
se a
ctiv
ity(a
rbitr
ary
units
)
- ALK1 ALK1+
Smad5
Smad5
140
160
180
-0
20
40
60
80
100
120
- ALK1-RNAi
Luci
fera
se a
ctiv
ity(a
rbitr
ary
units
)
MEEC BRE-lucMEEC BRE-luc
MEEC BRE-luc
+TGF--TGF-
+TGF--TGF-
+TGF--TGF-
