ACCURATE AND ROBUST

QUANTITATION OF V ITAMINS IN FOOD AND

BEVERAGES

[ VITAMIN ANALYSIS SOLUTIONS ]

can make baby’s dress &/or spoon green to match opposite page color.

[ VITAMIN ANALYSIS SOLUTIONS ]

Accurate and precise quantitative

measurements of vitamins are critical

for the manufacture of high quality

and regulatory compliant food and

beverage products.

Common vitamin analysis techniques are

often time-consuming and only target

a single vitamin per run. Manufacturers

recognize the need to employ more

efficient methodologies that are

appropriate for a wide variety of

food and beverage matrices.

Waters has pioneered the development

of accurate, robust, and easy-to-use

solutions for analyzing vitamins in foods,

beverages, and dietary supplements.

These comprehensive systems combine

the unparalleled performance of Waters®

ACQUITY UPLC® with advanced mass

spectrometry detection, innovative

sample preparation, and industry-leading

data management software, to help food

and beverage manufacturers increase

throughput and deliver quality, consistent

products, more cost-effectively than

ever before.

With fewer bottlenecks, less waste, and more

information in less time, Waters vitamin

analysis solutions allow you to easily

transfer and validate your current methods,

streamline analytical workflows, and help

reduce laboratory operational costs.

Waters systematically addresses

the specialized needs of vitamin

analysis for food and beverage

laboratories. Whatever the

challenge – SPE, batch-to-batch

column performance, method

repeatability, data management,

or system usability – we have

the solution.

SAMPLE PREPARATION AND CONSUMABLES

CONVERGENCE CHROMATOGRAPHY

ULTRA PERFORMANCE LIQUID CHROMATOGRAPHY

MASS SPECTROMETRY

DATA MANAGEMENT

WATERS GLOBAL SERVICES

Deliver quality, consistent products, more cost-effectively than ever before.

[ VITAMIN ANALYSIS SOLUTIONS ]

Efficient, cost-effective sample preparation for optimized laboratory performance

SAMPLE PREPARATION AND CONSUMABLES

OASIS SAMPLE EXTRACTION PRODUCTS

One of the most powerful sample preparation techniques is solid-phase extraction,

or SPE. Waters Corporation is a pioneer in this area and provides a variety of

industry leading products, applications, and technical expertise to our food

industry partners. Featuring the Oasis® sample extraction products and SPE

protocols, Waters food testing sample preparation products and consumables

provide the sensitivity, simplicity, and speed that today’s food and beverage

laboratories require.

■■ Provides simple, rapid,

and selective methods for

extracting vitamins from

fortified food products.

■■ Revolutionary polymeric and

silica-based SPE sorbents.

■■ Wide variety of formats

including cartridges, plates,

and on-line columns.

■■ Complete flexibility for

method development.

■■ Faster protocols allow reduced

time for vitamin extraction.

KEY FEATURES/BENEFITS

CONVERGENCE CHROMATOGRAPHY

ACQUITY UPC2 SYSTEM

Faster, more cost-effective, and sustainable vitamin analysis

ACQUITY UltraPerformance Convergence Chromatography (UPC2) System.

■■ ACQUITY UPC2™ provides improved

speed, resolution, reliability, and

flexibility compared to normal

phase LC, and reversed phase LC

for vitamin analyses.

■■ Leverages the unique properties of

supercritical carbon dioxide (CO2)

that provides a more cost-effective,

sustainable, and greener technology

alternative by lowering the use of

organic solvents.

■■ Reduce the number of assays for

laboratories routinely performing

multiple fat-soluble vitamin

analyses. Eliminates the need

for multiple methods typically

required by HPLC.

■■ ACQUITY UPC2 Columns have

excellent lifetimes, provide superior

peak shape, and offer a wide range

of selectivities.

With ACQUITY UPC2 the separation and analysis of seven fat soluble vitamins and two carotenoids can be achieved with a four-minute runtime.

A Acetate

E

K2

K1 E

D2

A Palmitate Lycopene

Beta carotene

AU

0.00 0.02 0.04 0.06 0.08 0.10 0.12 0.14 0.16 0.18 0.20 0.22 0.24 0.26 0.28 0.30 0.32 0.34 0.36 0.38 0.40

min

0.00 0.20 0.40 0.60 0.80 1.00 1.20 1.40 1.60 1.80 2.00 2.20 2.40 2.60 2.80 3.00 3.20 3.40 3.60 3.80 4.00

ACQUITY UltraPerformance Convergence Chromatography™ (UPC2®)

streamlines vitamin analysis by allowing labs to use a single technique

on a single system to analyze a wide range of analytes.

KEY FEATURES/BENEFITS

[ VITAMIN ANALYSIS SOLUTIONS ]

HPLC familiarity with UPLC performance

Allows you to easily update traditional HPLC

procedures by reducing multi-analyte analysis

times by up to 10-fold, while improving sensitivity,

resolution, and method robustness.

KEY FEATURES/BENEFITS

■■ Rapidly transfer analysis methods from

multiple HPLCs to one UPLC® System.

■■ The ACQUITY UPLC PDA and FLR

detectors provide fast data acquisition

capabilities for rapid analyses and the

sensitivity required for trace impurity

detection and quantification.

■■ Sensitivity, robust performance,

easy upkeep, and intuitive operation

for any expertise level with the

ACQUITY® QDa Detector.

■■ Reduce solvent consumption for LC

analyses by up to 95%, saving on

both acquisition and disposal costs.

■■ ACQUITY UPLC columns provide

unprecedented peak symmetry,

efficiency, and chemical stability.

■■ Generate certificates of analyses

(COAs) more rapidly to ensure

products are delivered to market

as quickly as possible.

ACQUITY UPLC H-Class System with ACQUITY QDa Detector.

ULTRAPERFORMANCE LIQUID CHROMATOGRAPHY

ACQUITY UPLC H-CLASS SYSTEM WITH ACQUITY QDa, PDA, AND FLR DETECTORS

min0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0 5.5 6.0 6.5 7.0

1

14**

13

12

11*

109

876*5

43

2

UV @ 270 nm* UV @ 205 nm** UV @ 625 nm

1. Thiamine-HCl (Vitamin B1)2. Ascorbic acid (Vitamin C)3. Nicotinic acid (Vitamin B3-OH)4. Niacinamide (Vitamin B3-NH2)5. Pyridoxine-HCl (Vitamin B6)6. Pantothenate (Vitamin B5)*7. Yellow #5 dye8. Cyanocobalamin (Vitamin B12)9. Folic acid (Vitamin B9)10. Caffeine11. Biotin (Vitamin B7)*12. Riboflavin (Vitamin B2)13. Red #40 dye14. Blue #1 dye**

Separation of 10 water soluble vitamins, caffeine, and three food dyes in less than seven minutes with the ACQUITY UPLC H-Class System.

Generate high-quality mass spectral data at the push of a button

With the ACQUITY QDa Detector, you now have information-rich mass spectral

data to complement data from your current Waters optical detectors. This

mass spectral information integrates seamlessly into the same workflow,

routinely giving you more complete separation characterization.

KEY FEATURES/BENEFITS

■■ Extend the sample detection ability

of your PDA detector to quantify

compounds with no UV response at

levels not amenable to or accessible

by optical detection.

■■ It’s as intuitive as an optical detector

with the robustness to handle all of

your analyses.

■■ Any analytical scientist can consistently

generate the highest quality mass

spectral data routinely – no special

training or expertise required.

ACQUITY QDa Detector.

ULTRAPERFORMANCE LIQUID CHROMATOGRAPHY

ACQUITY QDa DETECTOR

■■ The only mass detector that

integrates with and even fits on

top of your instrument stack.

■■ Compatible with all of Waters

ACQUITY UPLC, ACQUITY UPC, 2

Alliance® HPLC, and purification

LC and SFC systems.

■■ Fully compatible with Empower®

Chromatography Software

as well as MassLynx Mass

Spectrometry Software and

its suite of data processing

application managers.

[ VITAMIN ANALYSIS SOLUTIONS ]

MASS SPECTROMETRY

ACQUITY UPLC H-CLASS SYSTEM WITH XEVO TQD

Increased sensitivity and selectivity for routine analysis

Accessible UPLC/MS/MS applications for routine

quantitative analyses that reduces complexity,

increases ease-of-use, and maximizes productivity. ACQUITY UPLC H-Class System with Xevo TQD.

KEY FEATURES/BENEFITS

■■ IntelliStart™ Software simplifies MS

setup and routine operation with

automated quantification workflow

tools that minimize variability between

different operators, departments,

and laboratories.

■■ Observe MRM (multiple reaction

monitoring) data and full scan

background data simultaneously

for faster, more robust

method development.

■■ RADAR™ Technology provides the

sensitivity and selectivity to monitor

for matrix interferences, metabolites,

impurities, and degradants in samples

while accurately quantifying

target compounds.

■■ Universal ion source architecture

provides the most extensive range of

interface capabilities available.

■■ Ready-to-use UPLC/MS methods for

the analysis of water and fat soluble

vitamins within the Quanpedia™

compound database.

MASS SPECTROMETRY

ACQUITY UPLC I-CLASS SYSTEM WITH XEVO TQ-S

Delivers the ultimate sensitivity in LC/MS/MS performance

Allows for the analysis of multiple analytes and very complex

food and beverage matrices, where the highest levels of

sensitivity, selectivity, speed, and accuracy are required. ACQUITY UPLC I-Class System with Xevo TQ-S.

■■ StepWave™ ion guide: a revolutionary

new off-axis, ion-source technology,

delivers the ultimate LC/MS/MS

performance of any available tandem

quadrupole system.

■■ ScanWave™ technology, a novel

patented collision cell design, allows

scientists to get both the very best

quantitative data as well as superior

MS/MS spectral information.

■■ Streamline analysis of vitamins with

the use of multi-analyte methods.

■■ Easily detect the presence of

vitamins found at vastly differing

concentrations, such as Vitamin C

at high levels and Vitamin B12 at

extremely low levels, in the most

challenging matrices.

■■ Perform inter and intra batch

comparisons to monitor

manufacturing processes and

ensure product consistency.

KEY FEATURES/BENEFITS

Quantification of seven water soluble vitamins with the ACQUITY UPLC and Xevo TQ-S System. Calibration curves and residuals plots along with the MRM chromatograms of the lowest level standard and internal standard of each vitamin are shown.

Concentrations of the lowest level standard are listed below.

A: Folic acid, 1.14 ng/mL

B: Biotin, 1.18 ng/mL

C: Pantothenic acid, 8.40 ng/mL

D: Niacin, 14.17 ng/mL

E: Pyridoxine, 1.78 ng/mL

F: Riboflavin, 1.40 ng/mL

G: Thiamine, 2.10 ng/mL

min3.40 3.60 3.80 4.00 4.20 4.40 4.60 4.80

%

0

100 Folic Acid -IS3.64

65289.7

min

%

0

100 Folic Acid3.64

7481.3

Conc0 10 20 30 40 50 60 70 80 90 100 110

Resp

onse

0.0

2.5

5.0

7.5

10.0

12.5

Conc

Resi

dual

-4.00

-2.00

0.00

2.00

4.00

Conc0.0 5.0 10.0 15.0 20.0 25.0 30.0 35.0 40.0 45.0 50.0 55.0

Resp

onse

0.00

1.00

2.00

3.00

4.00

5.00

Conc

Resi

dual

-2.00

-1.00

0.00

1.00

min3.40 3.60 3.80 4.00 4.20 4.40 4.60 4.80

%

0

100 Biotin-IS (1)3.82

343941.2

min

%

0

100

Biotin3.82

36011.8

A: Folic acid

B: Biotin

Conc0 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800

Resp

onse

0.0

10.0

20.0

30.0

40.0

Conc

Resi

dual

-1.00

0.00

1.00

min2.00 2.10 2.20 2.30 2.40 2.50 2.60 2.70 2.80 2.90 3.00 3.10 3.20

%

0

100

Pantothenic Acid _IS2.58

243032.1

min

%

0

100

Pantothenic Acid2.58

94718.8

min1.200 1.300 1.400 1.500 1.600 1.700 1.800

%

0

100Niacin -IS;1.30;208859.3

min

%

0

100Niacin;1.32;27051.5

Conc0 100 200 300 400 500 600 700 800 900 1000 1100 1200 1300 1400

Resp

onse

0.0

2.5

5.0

7.5

10.0

Conc

Resi

dual

-1.00

0.00

1.00

C: Pantothenic acid

D: Niacin

Conc0 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170

Resp

onse

0.0

2.0

4.0

6.0

8.0

10.0

Conc

Resi

dual

-1.50

-1.00

-0.50

0.00

0.50

min2.00 2.10 2.20 2.30 2.40 2.50 2.60 2.70 2.80 2.90 3.00 3.10 3.20

%

0

100Pyridoxine-IS;2.32;339845.7

min

%

0

100

Pyridoxine2.32

37579.2

min3.40 3.60 3.80 4.00 4.20 4.40 4.60 4.80

%

0

100

Riboflavin-IS4.28

371230.3

min

%

0

100

Riboflavin4.29

36600.2

Conc0 10 20 30 40 50 60 70 80 90 100 110 120 130

Resp

onse

0.00

2.00

4.00

6.00

8.00

Conc

Resi

dual

-1.00

0.00

1.00

E: Pyridoxine

F: Riboflavin

Conc0 20 40 60 80 100 120 140 160 180 200

Resp

onse

0.00

2.00

4.00

6.00

8.00

Conc

Resi

dual

-1.00

0.00

1.00

min2.00 2.10 2.20 2.30 2.40 2.50 2.60 2.70 2.80 2.90 3.00 3.10 3.20

%

0

100

Thiamine-IS2.53

355830.3

min

%

0

100

Thiamine2.53

31958.9G: Thiamine

[ VITAMIN ANALYSIS SOLUTIONS ]

WATERS LABORATORY INFORMATICS SOFTWARE

Effective data management for vitamin analysis

Empower 3 Chromatography Data

Software makes it easier than ever to run

samples and produce meaningful results.

Empower 3’s interface is designed to

maximize your productivity, improving

how you collect, process and print

chromatography data. Grow seamlessly

from a single workstation to an enterprise-

wide system with Empower 3’s scalable,

modular architecture, which fits into the

most modern, secure, HA (high availability)

corporate architecture.

NuGenesis® 8 is a comprehensive data

management and workflow solution that

supports the entire product lifecycle

from discovery through manufacturing.

This user-centric platform encompasses

NuGenesis SDMS, a compliant-ready data

repository, and NuGenesis ELN, a flexible

analytical electronic laboratory notebook.

Capabilities such as sample management,

laboratory inventories, data retention

and legal hold, and electronic laboratory

execution methods, allow NuGenesis 8

to connect the scientific data from your

laboratories to the business systems of

your organization more effectively than

ever before.

MassLynx® Software acquires, analyzes,

and distributes Waters mass spectrometry

information for comprehensive instrument

control and data processing. To assist

with both data acquisition and the

transformation of data into usable results,

MassLynx Software offers easy-to-use

Application Managers that allow you to

focus the power of MS on your

laboratory’s specific tasks.

Paradigm™ Scientific Search is the

next-generation of search technology

for science-driven organizations –

Paradigm offers a solution for accessing

scientifically relevant information

(chemical structures, methods, chemical

reactions, chromatograms, spectra, etc.),

across an organization spanning

information silos, performing searches

based on science objects, and adding

structure to unstructured data.

A powerful suite of solutions for instrument control, scientific data management, scientific

search, network integration, and compliance management to streamline analytical

laboratory operations. Waters Informatics solutions have helped leading food and beverage

organizations reduce costs, accelerate decision making, improve laboratory effectiveness,

and get products to market faster.

WATERS GLOBAL SERVICES

Be assured. Choose Waters Global Services.

Waters Global Services focuses on

optimizing Waters products with

superior service, support, upgrades,

training, and Waters Quality Parts.®

Proven satisfaction

For thirteen consecutive years, an

independent quality auditing firm

ranked Waters Global Services

best-in-class in providing expert

technical knowledge, quick

resolution of system issues,

and process support.1

1 Achievement in Customer Excellence Award,

CustomerSat, Inc., 2007-2013; NorthFace

ScoreBoard Award,sm Omega Management

Group Corporation, 2001-2006.

“ The ‘Quality for Life’ vision at DSM

Nutritional Products focuses on

developing products and partnerships

where the essential values of

Quality, Reliability, Traceability, and

Sustainability are critical. Waters

UPLC Technology and Support services

align with our values, and give us

the confidence that we will continue

to provide the world ‘Bright Science.

Brighter Living,’ and remain the leader

in providing nutritional solutions.

I have worked with Waters Corporation for

decades and found that their personnel

and ‘green’ technology consistently

support these values and help protect our

‘Quality for Life’ promise to the world.”

TIM WELLSDirector of Quality, North America

www.waters.com/food

Waters, ACQUITY, ACQUITY UPLC, Alliance, Empower, MassLynx, NuGenesis, Oasis, The Science of What’s Possible, UPC,2 UPLC, Waters Quality Parts, and Xevo are registered trademarks of Waters Corporation. ACQUITY UPC,2 UltraPerformance Convergence Chromatography, Paradigm, RADAR, ScanWave, StepWave, Quanpedia, TrendPlot, and IntelliStart are trademarks of Waters Corporation. All other trademarks are the property of their respective owners.

©2013 Waters Corporation. Printed in the U.S.A.December 2013 720004386EN LM-IGS

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