58

SCLC HOo-SCLC Squamous Adeno Adeno-cyscic

c a r c . carc . carc . Kerazln 53 22124 43/66 21/21 &/4 vimentin 0/10 0/30 0/21 4 /4 Neurofilament 0/7 0 /4 123-(:3 25/25 10/74 0/21 4/4

"Adenoma" of the H~nan Peripheral Lung - Histological Electron Microscopic and Im- munohistochemical Studies of 5 Cases. Kodama, T., Shimosato, Y., Morinaga, S., Watanabe, S. National Cancer Center Research Institute, Tokyo, Japan.

In the human undamaged peripheral lung, we occasionally experience proliferative lesions with monotonous features, not ac- companied by ciliated cells but showing varying degrees of individual cell atypia but not exactly adenocarcinoma cells. One is forced to call such lesions "adenoma".

We had opportunities to study 5 such cases, the size of which varied from 1.2 to 1.7 cm in diameter. Three were detect-

ed by chest roentgenogram and were diffi- cult to differentiate from well differen- tiated adenocarcinoma. Two other lesions were incidentally found in surgical spe-

cimens resected for primary lung cancer. Histologically, they were made up of

cuboidal to low columnar or peg-shaped cells with slight to moderately atypical vesicular nuclei and small or inconspi- cuous nucleoli, resembling either type II alveolar epithelial cells or Clara cells. Cells were generally tightly join- ed together. Mitotic figures were rare.

Ultrastructurally, tumor cells consist- ed of type II alveolar epithelial cells, Clara cells and/or immature alveolar cub- oidal cells. Immunohistochemically, sur- factant apoprotein-positive tumor cells were focally or diffusely present in all five cases.

In one case, there was a focus composed of cells with more atypical nuclei, sug- gesting an adeno-carcinoma sequence.

Langerhans Cells and Their Precursors in Adenocarcinoma of the Lung in Relation to Postoperative Prognosis. Furukawa, T., Watanabe, S., Kodama, T., Sato, Y., Shimosato, Y., Suemasu, K. Na- tional Cancer Center Research Institute, Tokyo, Japan.

The infiltration of Langerhans cells and their precursors (L Cells) in lung

cancer was closely related to the infil- tration of T-lymphocytes and was found more frequently in adenocarcinoma than in other histological types of primary lung cancer (Cancer Res 43, 5883, 1983).

We studied infiltration of L Cells and macrophages by immunohistochemical methods using anti-Sl00 protein and anti-lysozyme antibodies in 40 stage Ia cases of adeno-

carcinoma of the lung. Varying population den-

sities of Sl00 + L Cells were demonstrated in

31 (77.5%) of 40 adenocarcinomas. The distri- bution of L Cells was clearly different from that of macrophages. Namely, the former was mainly interspersed among the tumor cells whereas macrophages were found in the stroma and around necrotic foci.

The prognosis of stage IA adenocarcinoma cases was related to the density of L Cells in tumor tissues. Cases with marked infiltra- tion of L Cells survived longer than those without or with only slight infiltration (P<0.05).

Such relationship was not observed with regard to macrophages. This indicate that L Cells infiltrating within the tumor may play a role in host defense mechanisms against tumor in the early stage of adenocarcinoma of the

lung.

Nuclear DNA in Adenocarcinoma and Squamous Cell Carcinoma of the Lung. Wiman, L-G, Auer, G., Nichimiya, K., Erhardt, K., Caspersson, T. Department of Lung Medicine,

Huddinge University Hospital, Huddinge and Dept. of Tumour Pathology, Karolinska Insti- tute, Stockholm, Sweden and Department of Sur- gery, Tokyo Medical College, Tokyo, Japan.

The purpose of this investigation was to determine the nuclear DNA profile in sur- gically removed lung tumours, which were pre- operatively diagnosed by rigid or flexible,

fiberoptic bronchoscopy. The patients had a survival time exceeding five years and show- ed no signs of distant metastases.

Material. From the National Swedish Can- cer Registry 58 patients were selected with a histologically verified diagnosis of in- vasive squamous cell carcinoma (n=33) or ade- nocarcinoma (n=25) and histologic sections

from well preserved tumour tissue suitable for DNA analysis.

Method. From each tumour one representa- tive block was chosen to provide consecutive sections for staining with hematoxylin-eosin and, after refixation in neutral formalin, according to the Feulgen-technique (acid hydro- lysis 5-N HCL, 22 ° C, 60 min). The nuclear DNA profile of single tumour cells was deter- mined by rapid scanning microspectrophotome- try.

Results. All of the squmous cell carci- nomas showed very high and scattered DNA- values indicative for aneuploidy, no one was diploid. Among the adenocarcinomas there were 36 percent exhibiting DNA content mainly with- in normal diploid range. The remaining adeno- carcinomas, however, showed aneuploid DNA- values similar to those of the squamous cell

type. Conclusion. The nuclear DNA content was

increased in squamous cell carcinoma, indica- ting aneuploidy. The adenocarcinomas, howe- ver, were more heterogenous showing both a