ADVANCED NUCLEAR MAGNETIC RESONANCE (ANMR)ADVANCED NUCLEAR MAGNETIC RESONANCE (ANMR)

UNIT 8UNIT 8HETERONUCLEAR CORRELATION NMR SPECTROMETRY HETERONUCLEAR CORRELATION NMR SPECTROMETRY

2D2D--NMRNMR

1. (1J )1H-13C CORRELATION: HETCOR / HMQC(HSQC) EXPERIMENTS

2. (>1J )1H-13C CORRELATION: HMBC EXPERIMENT

2 OTHER HETERONUCLEAR CORRELATIONS 1H 15N2. OTHER HETERONUCLEAR CORRELATIONS: 1H-15N

El autor/La autora se acoge al artículo 32 de la Ley de Propiedad Intelectual vigente respecto al uso parcial de obras ajenas, como imágenes, gráficos u otromaterial contenido en las diferentes diapositivas, dado el carácter y la finalidad exclusivamente docente y eminentemente ilustrativa de las explicaciones enclase de esta presentación. Facultad de Ciencias, Universidad de Alicante

BibliographyUnit 8: 2D NMR

Spectrometric Identification of Organic Compounds, 7th Ed.Robert M. Silverstein, Francis X. Webster, David J. Kiemle

Chapter 5: Correlation NMR Spectrometry; 2-D NMRChapter 5: Correlation NMR Spectrometry; 2-D NMRpp. 245-285

Introduction to Spectroscopy, 4th Ed.Donald L. Pavia, Gary M. Lampman, George S. Kriz, James R. Vyvyan

Chapter 10: Nuclear Magnetic Resonance SpectroscopyChapter 10: Nuclear Magnetic Resonance SpectroscopyPart Five: Advanced NMR Techniques

pp. 587-657

200 and More NMR ExperimentsA Practical Course

Stefan Berger Siegmar BraunStefan Berger, Siegmar BraunChapter 10: The Second Dimension

COSY (Correlated Spectroscopy)

Unit 8: 2D NMR

COSY (Correlated Spectroscopy)

DQF-COSY (Double Quantum Filtered Correlated Spectroscopy) SCALAR1H-1H

TOCSY (Totally Correlated Spectroscopy)

INADEQUATE (Incredible Natural Abundance Double Quantum Transfer) SCALARINADEQUATE (Incredible Natural Abundance Double Quantum Transfer)

HETCOR (Heteronuclear Correlation)

13C-13C (1J)

SCALAR13C 1H (1J)

( )

HMQC (HSQC) (Heteronuclear Multiple Quantum Correlation)

13C-1H (1J)

SCALAR1H 13C (1J)

( ) ( )

HMBC (Heteronuclear Multiple Bond Coherence) SCALAR1H-13C (> 1J)

1H-13C (1J)

NOESY (Nuclear Overhauser Enhancement Spectroscopy)

H- C (> J)

DIPOLAR

ROESY (Rotating Frame Overhauser Enhancement Spectroscopy)

DIPOLAR1H-1H

ADVANCED NUCLEAR MAGNETIC RESONANCE (ANMR)ADVANCED NUCLEAR MAGNETIC RESONANCE (ANMR)

UNIT 8UNIT 8HETERONUCLEAR CORRELATION NMR SPECTROMETRY HETERONUCLEAR CORRELATION NMR SPECTROMETRY

2D2D--NMRNMR

1. (1J) 1H-13C CORRELATION: HETCOR / HMQC(HSQC) EXPERIMENTS

2. (>1J ) 1H-13C CORRELATION: HMBC EXPERIMENT

3 OTHER HETERONUCLEAR CORRELATIONS 1H 15N3. OTHER HETERONUCLEAR CORRELATIONS: 1H-15N

1. 1J 1H-13C CORRELATION: HETCOR / HMQC(HSQC)

13

Unit 8: 2D NMR

13C DETECTION

1. 1J 1H-13C CORRELATION: HETCOR / HMQC(HSQC) Unit 8: 2D NMR

1H DETECTION

S/N: [NT2 γexc(γdetB0)3/2ns1/2)/T

Increased sensitivity ( / )5/2Increased sensitivity (γ1H/γ13C)5/2

1. 1J 1H-13C CORRELATION: HETCOR / HMQC(HSQC) Unit 8: 2D NMR

1. 1J 1H-13C CORRELATION: HETCOR / HMQC(HSQC) Unit 8: 2D NMR

1. 1J 1H-13C CORRELATION: HETCOR / HMQC(HSQC) Unit 8: 2D NMR

1. 1J 1H-13C CORRELATION: HETCOR / HMQC(HSQC) Unit 8: 2D NMR

1. 1J 1H-13C CORRELATION: HETCOR / HMQC(HSQC) Unit 8: 2D NMR

1. 1J 1H-13C CORRELATION: HETCOR / HMQC(HSQC) Unit 8: 2D NMR

1. 1J 1H-13C CORRELATION: HETCOR / HMQC(HSQC) Unit 8: 2D NMR

1. 1J 1H-13C CORRELATION: HETCOR / HMQC(HSQC) Unit 8: 2D NMR

1. 1J 1H-13C CORRELATION: HETCOR / HMQC(HSQC) Unit 8: 2D NMR

ADVANCED NUCLEAR MAGNETIC RESONANCE (ANMR)ADVANCED NUCLEAR MAGNETIC RESONANCE (ANMR)

UNIT 8UNIT 8HETERONUCLEAR CORRELATION NMR SPECTROMETRY HETERONUCLEAR CORRELATION NMR SPECTROMETRY

2D2D--NMRNMR

1. (1J) 1H-13C CORRELATION: HETCOR / HMQC(HSQC) EXPERIMENTS

2. (>1J ) 1H-13C CORRELATION: HMBC EXPERIMENT

3 OTHER HETERONUCLEAR CORRELATIONS 1H 15N3. OTHER HETERONUCLEAR CORRELATIONS: 1H-15N

2. >1J 1H-13C CORRELATION: HMBC EXPERIMENTUnit 8: 2D NMR

1H-13C: 2J and 3J

2. >1J 1H-13C CORRELATION: HMBC EXPERIMENTUnit 8: 2D NMR

2. >1J 1H-13C CORRELATION: HMBC EXPERIMENTUnit 8: 2D NMR

3

2. >1J 1H-13C CORRELATION: HMBC EXPERIMENTUnit 8: 2D NMR

2. >1J 1H-13C CORRELATION: HMBC EXPERIMENTUnit 8: 2D NMR

2. >1J 1H-13C CORRELATION: HMBC EXPERIMENT Unit 8: 2D NMR

2. >1J 1H-13C CORRELATION: HMBC EXPERIMENT Unit 8: 2D NMR

ADVANCED NUCLEAR MAGNETIC RESONANCE (ANMR)ADVANCED NUCLEAR MAGNETIC RESONANCE (ANMR)

UNIT 8UNIT 8HETERONUCLEAR CORRELATION NMR SPECTROMETRY HETERONUCLEAR CORRELATION NMR SPECTROMETRY

2D2D--NMRNMR

1. (1J ) 1H-13C CORRELATION: HETCOR / HMQC(HSQC) EXPERIMENTS

2. (>1J ) 1H-13C CORRELATION: HMBC EXPERIMENT

3 OTHER HETERONUCLEAR CORRELATIONS 1H 15N3. OTHER HETERONUCLEAR CORRELATIONS: 1H-15N

3. OTHER HETERONUCLEAR CORRELATIONS: 1H-15N Unit 8: 2D NMR

Unit 8: 2D NMR3. OTHER HETERONUCLEAR CORRELATIONS: 1H-15N