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dr. Agus Eka Darwinata, Ph.DGenetika Mikroorganisme
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The Central Dogma
Mutation
Gene dan Genome
Gene transfer
PCR
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Gene and Genome
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Genom adalah keseluruhan informasi genetik (gen) yang dimiliki suatu sel atau organisme.A
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Human GenomeA
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Bacterial GenomeA
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9181 bp
Viral GenomeA
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Genome-Gene AnalogyA
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The Central Dogma
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The Central Dogma of BiologyA
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DNA StructureA
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Replication
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Transcription
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Translation
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Translasi protein (cont.)A
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Mutation
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Mutation
! Changes in base sequece of DNA and inheritable"
! Can be:"! Harmful"! Lethal"! Helpful"! Silent
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Mutation
Point Mutation Frame Shift Mutation
missense
silent
nonsense
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Genetic Transfer
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Genetic Information Flow in BacteriaA
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Genetic Transfer
! Genetic transfer results in genetic variation"
! Genetic variation needed for evolution"
! Three ways:"! Transformation"! Conjugation"! Transduction
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Transformation! Genes transferred from one bacterium to another by virus.A
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Conjugation! Plasmid transferred from one bacterium to another via a pilus.A
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Conjugation cont…A
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PCR
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Polymerase Chain Reaction! PCR allows us to produce rapidly (amplify) a billion copies of DNA without needing a living cell. "
! These large quantities are then easily analyzed."
! PCR recipe:
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Polymerase Chain ReactionAmplification of nuc gene of S. aureus
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ResultA
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Thank you
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