-
8/7/2019 Aeromonas from Drinking Water in Tripoli
1/8
ISOALTION, HEMOLYTIC ACTIVITY AND
ANTIBIOTIC SUSCEPTIBILITY OF AEROMONAS SPECIES FROMDRINKING
WATER OBTAINED FROMWATER RESERVOIRS (FASGHIA) IN
TRIPOLI-LIBYA
Zakia M. El-Agili, Halima Nashnoush,Khalifa Sifaw Ghenghesh*,
and Amal Rahouma*
Dept. of Botany1, Faculty of Science, and*Dept. of Medical
Microbiology2, Faculty of Medicine,
Al-Fateh University, Tripoli-Libya
-
8/7/2019 Aeromonas from Drinking Water in Tripoli
2/8
I NTRODUCT I ON
AeromonasGram -ve, oxidase +ve bacilli.Habitat
Natural watersDisease
bacteremia,wound infections andgastroenteritis.
Virulence factorshemolysins,CytotoxinsAdherence
Mannose-resistant hemagglutination
-
8/7/2019 Aeromonas from Drinking Water in Tripoli
3/8
TreatmentResistant to F-lactams
Chromosomally mediatedCephalosporins, fluoroquinolones
Aims
Fasghia is an underground reservoir tocollect rain water used
for drinking.
To isolate Aeromonas species from drinking
water obtained from fasghias in Tripoli.To determine the
virulence factors of theisolated Aeromonas .To determine the
susceptibility of theisolated Aeromonas to antibiotics.
-
8/7/2019 Aeromonas from Drinking Water in Tripoli
4/8
MATER I AL AND METHODS
SamplesFrom October 2003-July 2004, 56 samples obtainedfrom
fasghias in Tripoli were examined
Microbiology
Samples were enriched in alkaline peptone water (APW, pH 8.6) at
37 oC.After overnight incubation, a loopful from APW wasplated on
ampicillin blood agar (ABA, 15mg/L).Plates were incubated at 37
oC.After overnight incubation, suspected colonies weretested for
oxidase and identified using standardmicrobiological procedures and
API20EIsolated Aeromonas were tested for hemolysinproduction on BA,
agglutination of RBCs in absenceand presence of mannose, and
susceptibility toantibiotics by the disc diffusion method.
-
8/7/2019 Aeromonas from Drinking Water in Tripoli
5/8
R ESULTS
Of 56 water samples examined, 41 (73%)were positive for
Aeromonas species.
Speciation of Aeromonas10 (24.4%) A. hydrophila ,10 (24.4%), A.
caviae ,9 (22%) A. sobria , and
12 (29.2%) atypical aeromonads .
Hemolysin production was detected inall (100%) Aeromonas
isolates.
-
8/7/2019 Aeromonas from Drinking Water in Tripoli
6/8
Of the 41 Aeromonas isolates
20 (48.8%) showed mannose-sensitivehemagglutination.14 (34.1%)
showed mannose-resistanthemagglutination.
-
8/7/2019 Aeromonas from Drinking Water in Tripoli
7/8
Resistance of Aeromonas from drinking water obtained from
fasghias in Tripoli-Libya
Antibiotic No (%) resistant(n =41)
----------------------------------------------------------------------Ampicillin
31 (75.6)
Amoxicillin + 14 (34.1)clavulanic acid
Amikacin 3 (7.3)Chloramphenicol 15 (36.6)
Ciprofloxacin 1 (2.4)Nalidixic acid 3 (7.3%)Tetracycline 1
(2.4%)Trimethoprim- 2 (4.8%)
sulphamethoxazole
-
8/7/2019 Aeromonas from Drinking Water in Tripoli
8/8
CONCLUS I ON
Antibiotic-resistant pathogenic Aeromonas species are common
indrinking water from water reservoirs (Fasghia) in Tripoli.
