Production · All equipments used for production as well as quality assurance are validated as per . prescribed guidelines. Equipment validation improves production processes and

ell culture is the science of growing cells derived from animal tissue or whole animals in an in vitro environment artificially made conducive to growth by addition of nutrients and growth factors.

First devised at the start of the 19th century, it has become one of the most widely used laboratory technique for the study of physiological and biochemical processes.

It is an attractive alternative to costly, time consuming animal studies involving drug interactions. Besides academic research, the importance of animal cell technology is emphasized again in the pharmaceutical sector where mammalian cell cultures have become the systems of choice for large scale production of desired proteins.

Cells are cultured in a culture medium, a chemically complex liquid suitable for supporting growth for several generations. A standard composition consists of salts, carbohydrates, vitamins, amino acids, growth factors, metabolic precursors, hormones and trace elements. Different cell lines vary in their requirement for the various nutrients. These differences are partly responsible for the extensive number of media formulations. Choice of a medium for a particular cell line is somewhat empirical.

To meet the requirements of the cell culture scientist, HiMedia supplies a wide range of media and reagents in both powder and liquid form. We have established ourselves as a manufacturer of high quality cell culture products for research, product development and industrial applications.

HiMedia Laboratories is in the business of Biotechnology since 1976. Ever since its inception, HiMedia has maintained a constant quest for excellence and a strong customer focused approach. We have earned an enviable reputation for our ability to provide consistently high quality products which are supported by our stringent quality management systems that are ISO 9001:2008 and ISO 13485:2003 certified. Our production facilities as well as quality control and R & D laboratories function in accordance with current WHO GMP (Good Manufacturing Practice) norms and our products are accredited with the CE mark.

Started primarily as manufacturers of bacteriological dehydrated culture media, we soon began catering to all the needs of microbiology for diagnostic, research and industrial purposes nationally and internationally. Our dedication to quality has enabled HiMedia to become a global name with customers in more than 140 countries.

In the recent years, we have gradually expanded and established ourselves in animal and plant tissue culture technology. As a research oriented company, we believe in consistently upgrading and increasing our product profile. Apart from being global suppliers of classical cell culture media, both in liquid and powder forms, we also supply balanced salt solutions in powder and liquid forms, cell culture reagents, sera, consumables and plasticware and various chemicals and biochemicals required for cell culture. We have a complete range of stable glutamine containing media, the HiGlutaXLTM range of media. Gradually, we are establishing ourselves in the serum free sector. We have the LoSeraTM range of media which are media requiring reduced supplementation with serum and serum free media for select cell lines. We have ventured in to the field of cytogenetics with our HiKaryoXLTM range of karyotyping media and reagents. As we try to meet the evolving needs of biologists, we are expanding in to the area of stem cells and media required for their expansion and differentiation.

Besides the standard catalogue listing, we are major suppliers of customized media. You can expect from us customized products especially suited to your needs at affordable prices. Special price breaks for larger volume purchases are also on offer.

Our customer needs demanded that we come out with an exclusive cross reference with other major suppliers in the world. Information provided herein is provided for general reference only, without any prejudice or liability, and is believed to be accurate on the day of completion. Users are advised to confirm their required specification with the information provided in product information sheet prior to selection of any product.

C

Production

All products are manufactured in accordance with WHO GMP, ISO 9001:2008 and ISO 13485:2003 guidelines. They are manufactured in special production areas where temperature and humidity is strictly controlled. Approved raw materials are transferred from raw material stores to production area. As part of the GMP process, appropriate check lists are provided at all the important junctions to counter check the correctness of the production procedures.

Raw materials

Only the highest quality raw materials are used in the production of Himedia products. All incoming raw materials are placed in quarantine until the completion of their testing by quality assurance department. Once they pass the given specifications, the lot is released for production. All raw materials are assigned unique codes and are designated separate lot numbers which help us to determine traceability of any product at any given time.

Raw materials are purchased from only approved vendors. Each supplier is evaluated for quality, availability, performance and service. We have controlled specifications for all our raw materials. Each supplier has to ascertain that their product meets our exact specification. Where necessary, we have a second list of back up suppliers.

Equipment validation

All equipments used for production as well as quality assurance are validated as per prescribed guidelines. Equipment validation improves production processes and assures consistency in the quality of the product. Each equipment is assigned a unique identification number. Each equipment also has a standard operating procedure for usage as well as calibration. The master plan for each equipment includes the operating specifications and ranges as well as the calibration record. We maintain a yearly planner for calibration and validation of all our equipments.

Production of powdered media

The raw materials are weighed by validated weighing balance. For powdered media, the raw materials are then milled in to fine particles of predetermined size, thus giving a product which dissolves easily and quickly. The powder is then sent to the packaging department for final packing. Controlled environmental conditions ensure that the powders coming out of the production area have the least amount of microbial load and endotoxin content.

Production of liquid media

Water purification

Since water forms the major raw material for all liquid products, we ensure that only the highest quality water is used for our liquid production. Water is subjected to serial processes which include water pretreatment, reverse osmosis, deionisation and distillation. Water used for our liquid production is water for injection grade, and meets all the criteria of USP monographs.

Filtration

Cell culture media cannot be terminally sterilized because of the heat labile nature of some of the constituents. Most of our liquid media are produced using pretested base powders. Where ever this is not feasible, the individual constituents are added in a predetermined sequence. The filtration and aseptic filling is carried out in validated class 100 stations into pre sterilized containers. The liquid production facility is a positive pressure, HEPA filtered, controlled environment facility.

Production Capacity

For powdered media, our manufacturing facility is equipped to take production in lot sizes varying from 1 kg to lots as large as 3000kg. Though our standard catalog packing is 1L, 5L and 20L, our bulk packaging varies as per the customer requirement. We can supply in pack sizes ranging from 500gms to 50kg. We have the capability to manufacture sterile liquid media in lot sizes starting from 5L to larger volumes.

Packing

Standard catalogue items for dry powdered media are available in 1litre, 5 litre and 20 litre packs. Bulk packing is available on request. Liquid media are available in standard 100ml and 500ml PET bottles. The standard packing comes in 20ml, 50ml, 100ml and 500ml bottles for sterile reagents. Liquid media packed in sterile bags shall be available on request.

Labelling

All our labels are done as per the CE norms. Beside the product code, name and description, label also includes product information regarding lot number, storage condition, grams/liter and expiry period.

Storage

All cell culture powdered media, salt mixtures and other concentrates are to be stored at 2-8°C in dark and dry conditions. Minimize light exposure to liquid media under both storage and usage conditions. Salt solutions are stored at ambient temperatures. Liquid media are to be stored at 2-8°C with exception of few media which are to be stored at -20°C. Trypsin and Trypsin-EDTA solutions are to be stored at -20°C. Most of the antibiotic solutions are to be stored at -20°C with exception of few antibiotics which are to be stored at 2-8°C. Please follow the label instructions for storage of individual products.

Certificate of Analysis

Every lot released for sale is provided with a detailed Certificate of Analysis which defines the product specifications and the actual test results. Hard copies of Certificate of Analysis are provided on request or can be directly downloaded from our website www.himedialabs.com.

Quality control

All our products undergo stringent quality control required to maintain consistency and quality. A statistically valid number of samples are withdrawn from each batch as per the defined procedure. We have documented procedures for quality control in accordance with GMP for in process as well as finished products.

We have developed in-house testing criteria for all our products and have defined release criteria. Each lot is passed only if the product conforms to the release criteria. For customized products, additional quality control testing is available on request. Following are some common parameters included in the routine testing protocols.

pH–pH test is performed on routinely calibrated instruments. Calibration is done using reference standards traceable to NIST.

Osmolality–Osmolality of solutions is determined using freezing point depression (FPD). The freezing point of a solution is depressed in direct relation to the amount of solute in solution.

Growth promotion test-Growth supporting property of is checked byanalyzing its effect on cell growth and morphology. Each test is conducted using a test sample and a validated control lot. Cell lines appropriate to each product are seeded at a known density and the logarithmic growth rate is monitored. Cultures are visually monitored at regular intervals to ensure characteristic cell morphology and rule out cytotoxicity.

Sterility–Each lot of sterile cell culture product is tested for absence of bacterial and fungal contaminants using methods referenced in current USP monographs for sterility testing. This involves inoculation in Tryptone soya broth, Thioglycollate broth and Sabouraud’s broth for 14 days and checking for contamination with reference to appropriate controls. All test media are validated by growth promotion test before use. Sera, in addition are tested for presence or absence of viruses using standard antibody-serum dilution tests.

Endotoxin–Endotoxin test is carried out using Limulus Amebocyte Lysate(LAL) assay.

Documented product specifications and defined testing protocols ensure all products meet their design specification. Control samples from each lot are retained as per the shelf life of individual product. This provision is made to make possible any follow up testing to comply to a customer request or for stability studies.

Stability Program–Shelf life of each product is determined after subjecting the product to stability study. Products are monitored at regular intervals and tested to check for conformance to product specifications. Stability program ensures that products deliver acceptable performance for the recommended shelf life when stored under prescribed conditions.

Chemical testing

Media / Balanced Salt

Biological testingPhysical testing

SolubilityClarity

OsmolarilitypH

Cell viabilityAppearance Cell growthEndotoxin

Quality control pass

Technical Service

Our dedicated team of experts will be pleased to answer your technical queries. At HiMedia, we believe customer is our top priority. Our team can provide you with expert product guidance and advice, troubleshooting tips, product information and protocols for all your cell culture needs. Please email us at [email protected] and [email protected] for all your technical queries. You can also log in your queries at www.himedialabs.com. You can call and fax us at the following numbers.

Fax :

91-22-4095 1920 / 91-22-25005764 / 91-22-25002468

Phone :

91-22-40951919 / 91-22-25003747

Registered Office : 23, Vadhani Industrial Estate, LBS Marg, Mumbai - 400 086, India. Tel. : (022) 6116 9797 / 2500 1607 Fax : (022) 2500 2286

Email : [email protected]

Website : www.himedialabs.com

Copyright & Related legal information

All rights reserved © HiMedia Laboratories Pvt. Limited, 2012. No part of this book may be reproduced by any mechanical, photographic or electronic process or in the form of a phonographic audio / audio visual recording, nor may it be stored in a retrieval system, transmitted or otherwise copied for public or private use, without written permission from the publisher.

Disclaimer : User must ensure of the product(s) in their application prior to use. Products conform solely to the information contained in this and other related HiMedia™ Publications. The information contained in this publication is based on our research and development work and is to the best of our knowledge true and accurate. Himedia™ Laboratories Pvt Ltd reserves the right to make changes to specifications and information related to the products at any time. Products are not intended for human or animal diagnostic or therapeutic use but for laboratory, research or further manufacturing use only, unless otherwise specified. Statements contained herein should not be considered as a warranty of any kind, expressed or implied, and no liability is accepted for infringement of any patents.

Limited Warranty : HiMedia will replace, or at a customer’s option credit customer’s account the purchase price for products that fail to meet the current published manufacturer’s specifications, provided such products are returned to HiMedia within 60 days of shipment.

Disclaimer of Warranty and Liability : HiMedia disclaims all other warranties or guarantees with respect to any products purchased from HiMedia, whether statutory, written, oral, express or implied, including, without limitation, any warranty or merchantability, suitability or fitness for a particular purchase. In no event shall HiMedia be liable or responsible for any exemplary, punitive, special, indirect, incidental or consequential damages (including, but not limited to loss of profits, use or goodwill) of any kind whether based on contract, tort (including negligence), strict liability, or any other theory or form of action even if HiMedia has been advised of the possibility thereof. HiMedia’s sole liability and customer’s sole remedy for any claim relating to any products purchased from HiMedia is as set forth in the limited warranty.

The word ‘HiMedia’, the logo of HiMedia as appearing in this booklet, For life is precious, ZymeFreeTM, HiGlutaXLTM, LoSeraTM, HiKaryoXLTM are Trade Marks owned by HiMedia Laboratories Pvt. Limited, Mumbai, India

Publication : 2012

Index

Basal Media 01

Insect Media 27

HiGlutaXLTM Media 29

LoSeraTM Media 30

Cell Freezing Media & Cytogenetics 31

Vitamin Solutions and Amino Acid Concentrates 32

Balanced Salts 33

Cell Dissociation Reagents 37

Antibiotic Solutions 39

Sera 41

Serum Free Growth Supplements 43

Miscellaneous Reagents 44

Cell Culture Kits 45

1

Information contained herein is provided for general reference only, without any prejudice or liability, and is believed to be accurate on the day of compilation.

Description

PrOduct HiMEdiaProduct Code

Sr.No.

invitrOGEnProduct Code

SiGMaProduct Code

LOnzaProduct Code

HycLOnEProduct Code

aME’S MEdiuM (POwdEr)

1 aME’s Medium AT121 - A1420 - - w/ L-Glutamine w/o Phenol red and Sodium bicarbonate

BGjB MEdiuM FittOn-jackSOn MOdiFicatiOn (POwdEr)

2 BGjb Medium Fitton-jackson Modification AT123 12591 - - - w/ L-Glutamine (Liquid) w/o Sodium bicarbonate

BaSaL MEdiuM EaGLE (BME) (POwdEr)

3 Basal Medium Eagle (BME) AT001 - B9638 - SH30159 w/ Earle’s salts and L-Glutamine w/o Sodium bicarbonate

4 Basal Medium Eagle (BME) AT061 - - - - w/ Earle’s salts, L-Glutamine and 25 mM HEPES buffer w/o Sodium bicarbonate

5 Basal Medium Eagle (BME) AT002 - - - - w/ Hank’s salts and L-Glutamine w/o Sodium bicarbonate

6 Basal Medium Eagle (BME) AT062 - - - - w/ Hank’s salts, L-Glutamine and 25 mM HEPES buffer w/o Sodium bicarbonate

7 Basal Medium Eagle (BME) (Modified for autoclaving) AT004A - - - - w/ Earle’s salts w/o L-Glutamine and Sodium bicarbonate

BaSaL MEdiuM EaGLE (BME) (Liquid)

8 Basal Medium Eagle (BME) AL002 - - - - w/ Hank’s salts and Sodium bicarbonate w/o L-Glutamine;

Basal Media

2


Description


Sr.No.


SiGMaProduct Code

LOnzaProduct Code

HycLOnEProduct Code

cLick’S MEdiuM (POwdEr)

9 click’s Medium (EHaa) Eagle Hank’s amino acids AT122A - - - - w/o L-Glutamine, Mercaptoethanol and Sodium bicarbonate

cLick’S MEdiuM (Liquid)

10 click’s Medium (EHaa) Eagle Hank’s amino acids AL117 0010188 C5572 - - w/Sodium bicarbonate w/o Mercaptoethanol and L-Glutamine

cMrL 1066 MEdiuM (POwdEr)

11 cMrL 1066 Medium (Powder) AT110 - C0422 - - w/ L-Glutamine w/o Sodium bicarbonate

cMrL 1066 MEdiuM (Liquid)

12 cMrL 1066 Medium (Liquid) AL110A *11530 - - - w/ L-Glutamine and Sodium bicarbonate

cHick EMBryO FiBrOBLaSt MEdiuM (POwdEr)

13 chick Embryo Fibroblast Medium AT168 - - - - w/ L-Glutamine w/o Sodium bicarbonate

cHick EMBryO FiBrOBLaSt MEdiuM (Liquid)

14 chick Embryo Fibroblast Medium AL168A - - - - w/ L-Glutamine and Sodium bicarbonate

duLBEccO’S MOdiFiEd EaGLE MEdiuM (dMEM) (POwdEr)

15 dulbecco’s Modified Eagle Medium (dMEM) AT063A - D5030 - - w/o L-Glutamine, Glucose, Phenol red, Sodium pyruvate and Sodium bicarbonate

*11530 : without Glutamine

Basal Media

3


Description


Sr.No.


SiGMaProduct Code

LOnzaProduct Code

HycLOnEProduct Code

16 dulbecco’s Modified Eagle Medium (dMEM) AT006 31600 D5523 - SH30002 w/ L-Glutamine, 1 gm Glucose per litre and Sodium pyruvate w/o Sodium bicarbonate

17 dulbecco’s Modified Eagle Medium (dMEM) AT006A - - - - w/ 1 gm Glucose per litre and Sodium Pyruvate w/o L-Glutamine and Sodium bicarbonate

18 dulbecco’s Modified Eagle Medium (dMEM) AT006F - - - - w/ 1gm Glucose per litre, L-Glutamine and Sodium pyruvate w/o Folic acid and Sodium bicarbonate

19 dulbecco’s Modified Eagle Medium (dMEM) AT149 - - - w/ L-Glutamine, 1 gm Glucose per litre, 25 mM HEPES buffer and Sodium Pyruvate w/o Sodium bicarbonate

20 dulbecco’s Modified Eagle Medium (dMEM) (Modified for autoclaving) AT065A - - - - w/ 1 gm Glucose per litre and Sodium pyruvate w/o L-Glutamine and Sodium bicarbonate

21 dulbecco’s Modified Eagle Medium (dMEM) AT007 12800 D7777 - SH30045 w/ L-Glutamine, 4.5 gm Glucose per litre and Sodium pyruvate w/o Sodium bicarbonate

22 dulbecco’s Modified Eagle Medium (dMEM) AT007F - - - - w/ L-Glutamine, 4.5 gm Glucose per litre and Sodium pyruvate w/o Folic acid and Sodium bicarbonate

23 dulbecco’s Modified Eagle Medium (dMEM) AT007A - - - - w/ 4.5 gm Glucose per litre and Sodium pyruvate w/o L-Glutamine and Sodium bicarbonate

24 dulbecco’s Modified Eagle Medium (dMEM) AT066 12100 D5648 15-604 SH30003 w/ 4.5 gm Glucose per litre and L-Glutamine w/o Sodium bicarbonate and Sodium pyruvate

25 dulbecco’s Modified Eagle Medium (dMEM) AT066A - - 15-614 SH30053 w/ 4.5 gm Glucose per litre w/o L-Glutamine, Sodium bicarbonate and Sodium pyruvate

26 dulbecco’s Modified Eagle Medium (dMEM) AT067 - D1152 - - w/ 4.5 gm Glucose per litre, L-Glutamine and 25 mM HEPES buffer w/o Sodium bicarbonate and Sodium pyruvate

27 dulbecco’s Modified Eagle Medium (dMEM) AT068 - - - - w/ 4.5 gm Glucose per litre and L-Glutamine w/o Sodium pyruvate, Sodium phosphate and Sodium bicarbonate

Basal Media

4


Description


Sr.No.


SiGMaProduct Code

LOnzaProduct Code

HycLOnEProduct Code

28 dulbecco’s Modified Eagle Medium (dMEM) AT151 - - - - w/ L-Glutamine, 4.5 gm Glucose per litre, Sodium Pyruvate and 25 mM HEPES buffer w/o Sodium bicarbonate

29 dulbecco’s Modified Eagle Medium (dMEM) AT170A - - - - w/ 4.5 gm Glucose per litre w/o L-Glutamine, Phenol red, Sodium bicarbonate and Sodium pyruvate

30 dulbecco’s Modified Eagle Medium (dMEM), High glucose AT170 - - - SH30211 w/ 4.5gms Glucose per litre and L-Glutamine w/o Phenol red, Sodium bicarbonate and Sodium pyruvate

31 dulbecco’s Modified Eagle Medium (dMEM) AT183 - D2902 - SH30044 w/ L-Glutamine, 1g per litre Glucose and Sodium pyruvate w/o Sodium bicarbonate and Phenol red

32 dulbecco’s Modified Eagle Medium (dMEM) AT183A - - - - w/ 1gm Glucose per litre and Sodium pyruvate w/o L-Glutamine, Phenol red and Sodium bicarbonate

33 dulbecco’s Modified Eagle Medium (dMEM) AT186 - - - - w/ L-Glutamine and Sodium pyruvate w/o Glucose and Sodium bicarbonate

34 dulbecco’s Modified Eagle Medium (dMEM) AT187 - - - - w/ 4.5gms Glucose per litre, 25mM HEPES, L-Glutamine and Sodium pyruvate w/o Sodium bicarbonate and Phenol red

35 dulbecco’s Modified Eagle Medium (dMEM), High glucose AT190 - - - - w/ 4.5gms Glucose per litre, L-Glutamine and Sodium pyruvate w/o Sodium bicarbonate and Phenol red

duLBEccO’S MOdiFiEd EaGLE MEdiuM (dMEM) (Liquid)

36 dulbecco’s Modified Eagle Medium (dMEM) AL111 - D5671 - - w/ 4.5 gms Glucose per litre and Sodium bicarbonate w/o L-Glutamine and Sodium pyruvate; Hybridoma tested 37 dulbecco’s Modified Eagle Medium (dMEM) AL151A - - - - w/ 4.5gm Glucose per litre, L-Glutamine, Sodium pyruvate, 25mM HEPES buffer and Sodium bicarbonate

38 dulbecco’s Modified Eagle Medium (dMEM) AL006 - D5546 12-707 - w/ 1.0 gm Glucose per litre, Sodium bicarbonate and Sodium pyruvate w/o L-Glutamine

Basal Media

5


Description


Sr.No.


SiGMaProduct Code

LOnzaProduct Code

HycLOnEProduct Code

39 dulbecco’s Modified Eagle Medium (dMEM) AL006A 11885 D6046 - SH30021 w/ 1gm Glucose per litre, L-Glutamine, Sodium pyruvate and Sodium bicarbonate

40 dulbecco’s Modified Eagle Medium (dMEM) AL006F - D2429* - - w/ 1gm Glucose per litre, L-Glutamine, Sodium pyruvate and Sodium bicarbonate w/o Folic acid

41 dulbecco’s Modified Eagle Medium (dMEM) AL149A 12320 - - - w/ 1.0 gm Glucose per litre, L-Glutamine, 25mM HEPES buffer, Sodium pyruvate and Sodium bicarbonate

42 dulbecco’s Modified Eagle Medium (dMEM) AL066 11960 - 12-733 SH30081 w/ 4.5 gms Glucose per litre and Sodium bicarbonate w/o L-Glutamine and Sodium pyruvate

43 dulbecco’s Modified Eagle Medium (dMEM) AL066A 11965 D5796 12-741 - w/ L-Glutamine, Sodium bicarbonate and 4.5gms Glucose per litre w/o Sodium pyruvate

44 dulbecco’s Modified Eagle Medium (dMEM) AL170 31053 D1145 - SH30585 w/ 4.5 gms Glucose per litre and Sodium bicarbonate w/o L-Glutamine, Sodium pyruvate and Phenol red

45 dulbecco’s Modified Eagle Medium (dMEM) AL007 10313 D6546 12-614 SH30285 w/ 4.5 gms Glucose per litre, Sodium bicarbonate and Sodium pyruvate w/o L-Glutamine

46 dulbecco’s Modified Eagle Medium (dMEM) AL007A 11995 D6429 12-604 SH30243 w/ 4.5 gms Glucose per litre, L-Glutamine, Sodium bicarbonate and Sodium pyruvate

47 dulbecco’s Modified Eagle Medium (dMEM) AL007F - - - - w/ 4.5gm Glucose per litre, L-Glutamine, Sodium pyruvate and Sodium bicarbonate w/o Folic acid

48 dulbecco’s Modified Eagle Medium(dMEM) AL007Sv - - - - w/ 4.5 gms Glucose per litre, 1mM Sodium pyruvate, L-Glutamine and 1.5 gms per litre of Sodium bicarbonate

49 dulbecco’s Modified Eagle Medium (dMEM) AL067A 12430 - - SH30249 w/ L-Glutamine, 4.5 gms Glucose per litre, 25mM HEPES buffer and Sodium bicarbonate w/o Sodium pyruvate

50 dulbecco’s Modified Eagle Medium (dMEM) AL068A 11971 - - - w/ L-Glutamine, 4.5 gms Glucose per litre and Sodium bicarbonate w/o Sodium pyruvate and Sodium phosphate

v As per ATCC composition

D2429* 10X without L-Glutamine and Sodium bicarbonate

Basal Media

6


Description


Sr.No.


SiGMaProduct Code

LOnzaProduct Code

HycLOnEProduct Code

51 dulbecco’s Modified Eagle Medium (dMEM) AL101 - - 12-917 SH30604 w/ 4.5 gms Glucose per litre, Sodium bicarbonate and Sodium pyruvate w/o Phenol red and L-Glutamine

52 dulbecco’s Modified Eagle Medium (dMEM) AL170A - - - SH30284 w/ L-Glutamine, Sodium bicarbonate and 4.5gms Glucose per litre w/o Sodium pyruvate and Phenol red

53 dulbecco’s Modified Eagle Medium (dMEM) AL183 11054 - - - w/ 1gm Glucose per litre, Sodium pyruvate and Sodium bicarbonate w/o L-Glutamine and Phenol red

54 dulbecco’s Modified Eagle Medium (dMEM) AL183A - - - - w/ 1gm Glucose per litre, L-Glutamine, Sodium pyruvate and Sodium bicarbonate w/o Phenol red

55 dulbecco’s Modified Eagle Medium (dMEM) AL186A - - - - w/ L-Glutamine, Sodium pyruvate and Sodium bicarbonate w/o Glucose

Basal Media

comparative analysis of performance of HiMedia dMEM vs competitor using Sp2/0 & cHO cell line

SP2/0 Ag14 cells were seeded in triplicate in a 48 well plate in AT007 and incubated at 370C with 5% CO2 and 95% air. Samples were taken every 24 hrs and evaluated for cell density and morphology. Cell growth and morphology of HiMedia was found comparable to the competitor medium.

CHO cells were seeded in triplicate in a 48 well plate in AT007 and incubated at 370C with 5% CO2 and 95% air. Samples were taken every 24 hrs and evaluated for cell density and morphology. Cell growth and morphology of HiMedia was found comparable to the competitor medium.

7


Description


Sr.No.


SiGMaProduct Code

LOnzaProduct Code

HycLOnEProduct Code

duLBEccO’S MOdiFiEd EaGLE MEdiuM / nutriEnt MixturE F-12 HaM (dMEM/F12, 1:1 MixturE) (POwdEr)

56 dulbecco’s Modified Eagle Medium / nutrient Mixture F-12 Ham (dMEM/F12, 1:1 mixture) AT127 - D0547 - - w/ L-Glutamine and Trace elements w/o HEPES buffer and Sodium bicarbonate

57 dulbecco’s Modified Eagle Medium / nutrient Mixture F-12 Ham (dMEM/F12, 1:1 mixture) AT127A - - - - w/ Trace elements w/o L-Glutamine, HEPES buffer and Sodium bicarbonate

58 dulbecco’s Modified Eagle Medium / nutrient Mixture F-12 (dMEM/F12, 1:1 mixture) AT147 - - - - w/ L-Glutamine and Trace elements w/o Phenol red, Sodium bicarbonate and HEPES buffer

59 dulbecco’s Modified Eagle Medium / nutrient Mixture F-12 Ham (dMEM / F12, 1:1 Mixture) AT147A - - - - w/ Trace elements w/o L-Glutamine, Phenol red, Sodium bicarbonate and HEPES buffer

60 dulbecco’s Modified Eagle Medium / nutrient Mixture F-12 Ham (dMEM/F12, 1:1 mixture) AT139 - - - - w/ L-Glutamine, 15 mM HEPES buffer and Trace elements w/o Sodium bicarbonate

61 dulbecco’s Modified Eagle Medium / nutrient Mixture F-12 Ham (dMEM/F12, 1:1 mixture) AT139A - - - - w/ HEPES buffer and Trace elements w/o L-Glutamine and Sodium bicarbonate

62 dulbecco’s Modified Eagle Medium / nutrient Mixture F-12 Ham (dMEM/F12, 1:1 mixture) AT140 12400 D8900 - SH30004 w/ L-Glutamine and 15mM HEPES buffer w/o Trace elements and Sodium bicarbonate

63 dulbecco’s Modified Eagle Medium / nutrient Mixture F-12 Ham (dMEM/F12, 1:1 mixture) AT140A - - - - w/ 15mM HEPES buffer w/o L-Glutamine, Trace elements and Sodium bicarbonate

64 dulbecco’s Modified Eagle Medium / nutrient Mixture F-12 Ham (dMEM/F12, 1:1 mixture) AT165 - D2906 - - w/15 mM HEPES buffer and L-Glutamine w/o Phenol red, Sodium bicarbonate and Trace elements

65 dulbecco’s Modified Eagle Medium / nutrient Mixture F-12 Ham (dMEM/F12, 1:1 mixture) AT155 12500 - - SH30069 w/ L-Glutamine w/o HEPES buffer,Trace elements and Sodium bicarbonate

66 dulbecco’s Modified Eagle Medium / nutrient Mixture F-12 Ham (dMEM/F12, 1:1 mixture) AT155A - - - - w/o L-Glutamine, HEPES buffer, Trace elements and Sodium bicarbonate

Basal Media

8


Description


Sr.No.


SiGMaProduct Code

LOnzaProduct Code

HycLOnEProduct Code

67 dulbecco’s Modified Eagle Medium/ nutrient mixture F-12 Ham (dMEM/ F12, 3:1 Mixture) AT189 - - - - w/ L-Glutamine w/o Calcium chloride, HEPES buffer and Sodium bicarbonate

duLBEccO’S MOdiFiEd EaGLE MEdiuM / nutriEnt MixturE F-12 HaM (dMEM/F12, 1:1 MixturE) (Liquid)

68 dulbecco’s Modified Eagle Medium / nutrient Mixture F-12 Ham (dMEM/F12, 1:1 mixture) AL127 - - - - w/ Sodium bicarbonate and Trace elements w/o L-Glutamine and HEPES buffer

69 dulbecco’s Modified Eagle Medium / nutrient Mixture F-12 Ham (dMEM/F12, 1:1 mixture) AL127A - - - - w/ L-Glutamine, Sodium bicarbonate and Trace elements w/o HEPES buffer

70 dulbecco’s Modified Eagle Medium / nutrient Mixture F-12 (dMEM F12,1:1 Mixture) AL155A 11320 - - - w/ L-Glutamine and 2.438 gms per litre Sodium bicarbonate w/o HEPES buffer and Trace elements

71 dulbecco’s Modified Eagle Medium / nutrient Mixture F-12 (dMEM F12,1:1 Mixture) AL158 - - - - w/ Sodium bicarbonate w/o L-Glutamine, HEPES buffer and Trace elements

72 dulbecco’s Modified Eagle Medium / nutrient Mixture F-12 Ham (dMEM/F12, 1:1 Mixture) AL127S - - - - w/ Trace elements, L-Glutamine and 1.5gms per litre Sodium bicarbonate w/o HEPES buffer

73 dulbecco’s Modified Eagle Medium / nutrient Mixture F-12 Ham (dMEM/F12, 1:1 mixture) AL139 - - - - w/ 15 mM HEPES buffer, Sodium bicarbonate and Trace elements w/o L-Glutamine

74 dulbecco’s Modified Eagle Medium / nutrient Mixture F-12 (dMEM/F12, 1:1 Mixture) AL139A - - - - w/ L-Glutamine, 15 mM HEPES buffer, Sodium bicarbonate and Trace elements

75 dulbecco’s Modified Eagle Medium / nutrient Mixture F-12 (dMEM/F12, 1:1 Mixture) AL140 - D6421 - SH30126 w/ 15 mM HEPES buffer and Sodium bicarbonate w/o L-Glutamine and Trace elements

76 dulbecco’s Modified Eagle Medium/nutrient F-12 Ham (dMEM/F12, 1:1 Mixture) AL140S 11330 D8437 12-719 SH30023 w/ 2.5mM L-Glutamine, 15mM HEPES, 0.5mM Sodium pyruvate and 1.2gms per litre Sodium bicarbonate

77 dulbecco’s Modified Eagle Medium/ nutrient Mixture F-12 Ham (dMEM/F12, 1:1 Mixture) AL187 - - - - w/ 15mM HEPES Buffer, Trace elements and Sodium bicarbonate w/o L-Glutamine and Phenol red

Basal Media

9


Description


Sr.No.


SiGMaProduct Code

LOnzaProduct Code

HycLOnEProduct Code

78 dulbecco’s Modified Eagle Medium/ nutrient Mixture F-12 Ham (dMEM/F12, 1:1 Mixture) AL187A - - - - w/ 15mM HEPES Buffer, L-Glutamine, Trace elements and Sodium bicarbonate w/o Phenol red

79 dulbecco’s Modified Eagle Medium/ nutrient Mixture F-12 Ham (dMEM/F12, 1:1 Mixture) AL188A - - - - w/ L-Glutamine, Trace elements and Sodium bicarbonate w/o HEPES Buffer and Phenol red

FiScHEr’S MEdiuM

80 Fischer’s Medium AT054 11475 - - - w/ L-Glutamine (Liquid) w/o Sodium bicarbonate

GLaSGOw’S MiniMuM ESSEntiaL MEdiuM (GMEM) (POwdEr)

81 Glasgow’s Minimum Essential Medium (GMEM) AT102 - G6148 - SH30269 w/ L-Glutamine w/o Tryptose phosphate broth and Sodium bicarbonate

82 Glasgow’s Minimum Essential Medium (GMEM) AT058 - - - - w/ L-Glutamine and NEAA w/o Sodium phosphate, Sodium bicarbonate and Tryptose Phosphate Broth

83 Glasgow’s Minimum Essential Medium (GMEM) AT069 - - - - w/ L-Glutamine and Tryptose phosphate broth w/o Sodium bicarbonate

GLaSGOw’S MiniMuM ESSEntiaL MEdiuM (GMEM) (Liquid)

84 Glasgow’s Minimum Essential Medium (GMEM) AL058 - - - - w/ Sodium bicarbonate and NEAA w/o L-Glutamine, Sodium phosphate and Tryptose Phosphate Broth

85 Glasgow’s Minimum Essential Medium (GMEM) AL069 - - - - w/ Tryptose phosphate broth and Sodium bicarbonate w/o L-Glutamine

86 Glasgows Minimum Essential Medium AL166A 11710 - 12-739 - w/ L-Glutamine and Sodium Bicarbonate w/o Tryptose Phosphate Broth

Basal Media

10


Description


Sr.No.


SiGMaProduct Code

LOnzaProduct Code

HycLOnEProduct Code

iScOvE’S MOdiFiEd duLBEccO’S MEdiuM (iMdM) (POwdEr)

87 iscove’s Modified dulbecco’s Medium (iMdM) AT160 - - - - w/ L-Glutamine w/o HEPES buffer and Sodium bicarbonate

88 iscove’s Modified dulbecco’s Medium (iMdM) AT070 12200 I7633 - SH30005 w/ L-Glutamine and 25 mM HEPES buffer w/o Sodium bicarbonate

89 iscove’s Modified dulbecco’s Medium (iMdM) AT070A - - - SH30380 w/ 25mM HEPES buffer w/o L-Glutamine and Sodium bicarbonate

90 iscove’s Modified dulbecco’s Medium (iMdM) (For Suspension culture) AT116 - - - - w/ L-Glutamine and 25 mM HEPES buffer w/o Calcium chloride and Sodium bicarbonate

91 iscove’s Modified dulbecco’s Medium (iMdM) AT188 - - - - w/ L-Glutamine and 25mM HEPES Buffer w/o Sodium bicarbonate and Phenol red

iScOvE’S MOdiFiEd duLBEccO’S MEdiuM (iMdM) (Liquid)

92 iscove’s Modified dulbecco’s Medium (iMdM) AL070 - - 12-726 SH30259 w/ Sodium bicarbonate and 25mM HEPES buffer w/o L-Glutamine

93 iscove’s Modified dulbecco’s Medium (iMdM) AL070H - I6529 12-915 - w/ L-Glutamine, Sodium bicarbonate and 25mM HEPES buffer Hybridoma tested 94 iscove’s Modified dulbecco’s Medium (iMdM) AL070B - I3390 - - w/ Sodium bicarbonate and 25mM HEPES buffer w/o L-Glutamine Hybridoma tested 95 iscove’s Modified dulbecco’s Medium (iMdM) AL070Sv - - - - w/ 4mM L-Glutamine, 4.5 gms Glucose per litre, 25 mM HEPES buffer and 1.5 gms per litre Sodium bicarbonate

96 iscove’s Modified dulbecco’s Medium (iMdM) AL070A 12440 - 12-722 SH30228 w/ L-Glutamine, 3.024 gms per litre Sodium bicarbonate and 25mM HEPES buffer


Basal Media

11


Description


Sr.No.


SiGMaProduct Code

LOnzaProduct Code

HycLOnEProduct Code

LEiBOvitz’S L-15 MEdiuM (POwdEr)

97 Leibovitz’s L-15 Medium AT011 41300 L4386 - SH30048 w/ L-Glutamine w/o Sodium bicarbonate

98 Leibovitz’s L-15 Medium AT011A - - - - w/o L-Glutamine and Sodium bicarbonate

99 Leibovitz’s L-15 Medium AT164 - - - - w/ L-Glutamine w/o Sodium Pyruvate and Sodium bicarbonate

LEiBOvitz’S L-15 MEdiuM (Liquid)

100 Leibovitz’s L-15 Medium AL011 - L5520 12-700 - w/o L-Glutamine

101 Leibovitz’s L-15 Medium AL011Sv 11415 L1518 - SH30525 w/ 2mM L-Glutamine w/o Sodium bicarbonate

MccOy’S 5a MEdiuM (POwdEr)

102 Mccoy’s 5a Medium AT057 - M4892 - SH30049 w/ L-Glutamine w/o Sodium bicarbonate

103 Mccoy’s 5a medium AT057A - - - - w/o L-Glutamine and Sodium bicarbonate

104 Mccoy’s 5a Medium (For Suspension culture) AT013 - - - - w/ L-Glutamine w/o Folic acid, Calcium chloride and Sodium bicarbonate

105 Mccoy’s 5a Medium (For Suspension culture) AT071 - - - - w/ L-Glutamine w/o Calcium chloride and Sodium bicarbonate

106 Mccoy’s 5a Medium AT179 - - - - w/ L-Glutamine w/o Phenol red and Sodium bicarbonate


Basal Media

12


Description


Sr.No.


SiGMaProduct Code

LOnzaProduct Code

HycLOnEProduct Code

MccOy’S 5a MEdiuM (Liquid)

107 Mccoy’s 5a Medium AL057 - M8403 - - w/ Sodium bicarbonate w/o L-Glutamine

108 Mccoy’s 5a Medium AL057A 16600 M9309 12-688 SH30200 w/ L-Glutamine and Sodium bicarbonate

109 Mccoy’s 5a Medium AL057H - - - - w/ L-Glutamine and Sodium bicarbonate Hybridoma tested 110 Mccoy’s 5a Medium AL057Sv - - - - w/ 1.5mM L-Glutamine and 2.2 gms Sodium bicarbonate per litre

McdB MEdiuM (POwdEr)

111 McdB 105 Medium AT131 - M6395 - - w/ Trace elements, L-Glutamine and 25mM HEPES buffer w/o Sodium bicarbonate

112 McdB 110 Medium AT132 - - - - w/ Trace elements, L-Glutamine and 25mM HEPES buffer w/o Sodium bicarbonate

113 McdB 131 Medium AT133 - M8537 - - w/ Trace elements and L-Glutamine w/o Sodium bicarbonate

114 McdB 151 Medium AT134 - - - - w/ Trace elements, L-Glutamine and 28mM HEPES buffer w/o Sodium bicarbonate



117 McdB 302 Medium AT137 - - - - w/ Trace elements and L-Glutamine w/o Nucleosides and Sodium bicarbonate

Basal Media


13


Description


Sr.No.


SiGMaProduct Code

LOnzaProduct Code

HycLOnEProduct Code

McdB MEdiuM (Liquid)

118 McdB 131 Medium (Liquid) AL133A 10372* - - - w/ Trace elements, L-Glutamine and Sodium bicarbonate

MEdiuM 199 (POwdEr)

119 Medium 199 AT014 31100 M5017 - SH30297 w/ Earle’s salts and L-Glutamine w/o Sodium bicarbonate

120 Medium 199 AT014A - - - - w/ Earle’s salts w/o L-Glutamine and Sodium bicarbonate

121 Medium 199 (For Suspension culture) AT043 - - - - w/ Earle’s salts and L-Glutamine w/o Calcium chloride, Magnesium sulphate and Sodium bicarbonate

122 Medium 199 AT015 - M0393 - - w/ Hank’s salts and L-Glutamine w/o Sodium bicarbonate

123 Medium 199 (For Suspension culture) AT044 - - - - w/ Hank’s salts and L-Glutamine w/o Calcium chloride, Magnesium sulphate and Sodium bicarbonate

124 Medium 199 AT094 - M2520 - - w/ Earle’s Salts, L-Glutamine and 25 mM HEPES buffer w/o Sodium bicarbonate

125 Medium 199 AT094A - - - - w/ Earle’s Salts and 25 mM HEPES buffer w/o L-Glutamine and Sodium bicarbonate

MEdiuM 199 (Liquid)

126 Medium 199 AL014 - M2154 - - w/ Earle’s Salts and Sodium bicarbonate w/o L-Glutamine

127 Medium 199 (For Suspension culture) AL043A - - - - w/ Earle’s, L-Glutamine and Sodium bicarbonate w/o Calcium chloride and Magnesium sulphate

Basal Media

10372* : without L-Glutamine

14


Description


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SiGMaProduct Code

LOnzaProduct Code

HycLOnEProduct Code

128 Medium 199 AL015 - M7653 - - w/ Hank’s Salts and Sodium bicarbonate w/o L-Glutamine

129 Medium 199 AL094 - M7528 - - w/ Earle’s salts, 25 mM HEPES buffer and Sodium bicarbonate w/o L-Glutamine

130 Medium 199 AL195A 12350 - - - w/ Hank’s Salts, 25mM HEPES Buffer, L-Glutamine and Sodium bicarbonate

131 Medium 199 AL094A 12340 - 12-117 - w/ Earle’s salts, 25 mM HEPES buffer, L-Glutamine and Sodium bicarbonate

132 Medium 199, 10x AL189 11825 M0650 - - w/ Earle’s Salts w/o L-Glutamine and Sodium bicarbonate

133 Medium 199, 10x AL192 - M9163 - - w/ Hank’s Salts w/o L-Glutamine and Sodium bicarbonate

MiniMuM ESSEntiaL MEdiuM (POwdEr)

134 Minimum Essential Medium Eagle (MEM) AT020 61100 M0268 15-611 SH30008 w/ Earle’s salts and L-Glutamine w/o NEAA and Sodium bicarbonate

135 Minimum Essential Medium Eagle (MEM) AT159 - - - SH30171 w/ Earle’s salts and L-Glutamine w/o Sodium bicarbonate, NEAA and Phenol Red

136 Minimum Essential Medium Eagle (MEM) AT047 41500 M0643 - SH30050 w/ Earle’s salts, NEAA and L-Glutamine w/o Sodium bicarbonate

137 Minimum Essential Medium Eagle (MEM) AT047A - - - - w/ Earle’s salts and NEAA w/o L-Glutamine and Sodium bicarbonate

138 Minimum Essential Medium Eagle (MEM) AT046 - - - - w/ Earle’s salts, NEAA and L-Glutamine w/o Phenol red and Sodium bicarbonate

Basal Media

15


Description


Sr.No.


SiGMaProduct Code

LOnzaProduct Code

HycLOnEProduct Code

139 Minimum Essential Medium Eagle (MEM) AT046A - M3024 - - w/ Earle’s salts and NEAA w/o Phenol red, L-Glutamine and Sodium bicarbonate

140 Minimum Essential Medium Eagle (MEM) AT153 - - - - w/ Earle’s salts, NEAA, L-Glutamine and 25mM HEPES buffer w/o Sodium bicarbonate

141 Minimum Essential Medium Eagle (MEM) AT154 - - - - w/ Earle’s salt, L-Glutamine, NEAA and Sodium pyruvate w/o Sodium bicarbonate

142 Minimum Essential Medium Eagle (MEM) AT074 - M2645 - - w/ Earle’s salts, L-Glutamine and 25 mM HEPES buffer w/o Sodium bicarbonate and NEAA

143 Minimum Essential Medium Eagle (MEM) AT182 - - - - w/ Earle’s salts, L-Glutamine, NEAA, 15mM HEPES buffer and Sodium pyruvate w/o Sodium bicarbonate

144 Minimum Essential Medium Eagle (MEM) AT056 - M4642 - SH30193 w/ Hank’s salts and L-Glutamine w/o Sodium bicarbonate

145 Minimum Essential Medium Eagle (MEM) AT056A - - - - w/ Hank’s salts w/o L-Glutamine and Sodium bicarbonate

146 Minimum Essential Medium Eagle (MEM) AT048 - M1018 - - w/ Hank’s salts, L-Glutamine and NEAA w/o Sodium bicarbonate

147 Minimum Essential Medium Eagle (MEM) AT075 - - - - w/ Hank’s salts, L-Glutamine and 25 mM HEPES buffer w/o Sodium bicarbonate

148 Minimum Essential Medium Eagle (MEM) (Modified for autoclaving) AT017A - - - - w/ Earle’s salts and NEAA w/o L-Glutamine and Sodium bicarbonate

149 Minimum Essential Medium Eagle (MEM) (Modified for autoclaving) AT077A 11700 M0769 - SH30327 w/ Earle’s salts w/o L-Glutamine, NEAA and Sodium bicarbonate

150 Minimum Essential Medium Eagle (MEM) (Modified for autoclaving) AT045A - - - - w/ Hank’s salts and NEAA w/o L-Glutamine and Sodium bicarbonate

Basal Media

16


Description


Sr.No.


SiGMaProduct Code

LOnzaProduct Code

HycLOnEProduct Code

151 Minimum Essential Medium Eagle (MEM) (joklik Modification) (For Suspension culture) AT079 - M0518 - - w/ L-Glutamine w/o Calcium chloride and Sodium bicarbonate

152 Minimum Essential Medium Eagle (MEM) (alpha Modification) AT080 11900 M0644 - SH30007 w/ L-Glutamine, Ribonucleosides and Deoxyribonucleosides w/o Sodium bicarbonate

153 Minimum Essential Medium Eagle (MEM) (alpha Modification) AT080A - - - - w/ Ribonucleosides and Deoxyribonucleosides w/o L-Glutamine and Sodium bicarbonate

154 Minimum Essential Medium Eagle (MEM) (alpha Modification) AT081 12000 M0894 - SH30219 w/ L-Glutamine w/o Deoxyribonucleosides, Ribonucleosides and Sodium bicarbonate

155 Minimum Essential Medium Eagle (MEM) (alpha Modification) AT081A - - - SH30205 w/o L-Glutamine, Deoxyribonucleosides, Ribonucleosides and Sodium bicarbonate

MiniMuM ESSEntiaL MEdiuM (Liquid)

156 Minimum Essential Medium Eagle (MEM) 10x AL104 - - - - w/ Earle’s salts and NEAA w/o Sodium bicarbonate and L-Glutamine

157 Minimum Essential Medium Eagle (MEM) AL056 - M5775 12-127 - w/ Hank’s salts and Sodium bicarbonate w/o L-Glutamine

158 Minimum Essential Medium Eagle (MEM) AL056A 11575 M4780 - - w/ Hank’s salts, L-Glutamine and Sodium bicarbonate

159 Minimum Essential Medium Eagle (MEM) AL020 11090 M2279 12-125 SH30244 w/ Earle’s salts and Sodium bicarbonate w/o NEAA and L-Glutamine

160 Minimum Essential Medium Eagle (MEM) AL020A 11095 M4655 12-611 SH30024 w/ Earle’s salts, L-Glutamine and Sodium bicarbonate w/o NEAA

161 Minimum Essential Medium Eagle (MEM) AL047 10370 M5650 - - w/ Earle’s salts, NEAA and Sodium bicarbonate w/o L-Glutamine

162 Minimum Essential Medium Eagle (MEM) AL047A - - - - w/ Earle’s salts, L-Glutamine, NEAA and Sodium bicarbonate

Basal Media

17


Description


Sr.No.


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LOnzaProduct Code

HycLOnEProduct Code

163 Minimum Essential Medium Eagle (MEM) AL047H - - - - w/ Earle’s salts, NEAA, Sodium bicarbonate and L-Glutamine Hybridoma tested 164 Minimum Essential Medium Eagle AL047S - - - - w/ 2mM L-Glutamine, 1mM Sodium pyruvate, NEAA and 1.5 gms per litre Sodium bicarbonate

165 Minimum Essential Medium Eagle (MEM) AL182A - - - - w/ Earle’s salts, L-Glutamine, NEAA, 15mM HEPES buffer, Sodium pyruvate and Sodium bicarbonate

166 Minimum Essential Medium Eagle (MEM) (For Suspension culture) AL019 11380 M8167 - - w/ Spinner salts and Sodium bicarbonate w/o L-Glutamine

167 Minimum Essential Medium Eagle (alpha Modification) AL081 - M4526 12-169 SH30568 w/ Sodium bicarbonate w/o L-Glutamine, Deoxyribonucleosides and Ribonucleosides

168 Minimum Essential Medium Eagle ( alpha Modification) AL081A 12561 - - - w/ L-Glutamine and Sodium bicarbonate w/o Deoxyribonucleosides and Ribonucleosides

169 Minimum Essential Medium Eagle (alpha modification) AL080 - M8042 - - w/ Deoxyribonucleosides, Ribonucleosides and Sodium bicarbonate w/o L-Glutamine

170 Minimum Essential Medium Eagle ( alpha Modification) AL080A 12571 - - SH30265 w/ Deoxyribonucleosides, Ribonucleosides, L-Glutamine and Sodium bicarbonate

171 Minimum Essential Medium Eagle (MEM) (joklik Modification) (For Suspension culture) AL079 - M8028 *04-719 - w/ Sodium bicarbonate w/o L-Glutamine

172 Minimum Essential Medium Eagle AL075A - - - - w/ Hank’s Salts, HEPES buffer, L-Glutamine and Sodium bicarbonate

173 Minimum Essential Medium Eagle 2x AL178A - - 12-668 - w/ Earle’s salt, L-Glutamine, Sodium bicarbonate and NEAA w/o Phenol red

174 Minimum Essential Medium Eagle (MEM) AL154A - - - - w/ Earle’s salt, L-Glutamine, NEAA, Sodium pyruvate and Sodium bicarbonate

175 Minimum Essential Medium Eagle (MEM) 10x AL190 - M9288 - - w/ Hank’s salts w/o L-Glutamine and Sodium bicarbonate

176 Minimum Essential Medium Eagle (MEM) 10x AL191 11430 M0275 12-684 - w/ Earle’s salts w/o NEAA, L-Glutamine and Sodium bicarbonate

Basal Media

*04-719 : with Glutamine

18


Description


Sr.No.


SiGMaProduct Code

LOnzaProduct Code

HycLOnEProduct Code

177 Minimum Essential Medium Eagle (MEM) AL020S - - - - w/ Earle’s salts and 1.5 gms per litre Sodium bicarbonate w/o NEAA and L-Glutamine

178 Minimum Essential Medium Eagle (MEM) AL074 12360 M7278 12-136 - w/ Earle’s salts, 25mM HEPES buffer and Sodium bicarbonate w/o NEAA and L-Glutamine

Basal Media

comparative analysis of performance of HiMedia MEM vs competitor using BHk & vero cell line

BHK - 21 cells were seeded in triplicate in a 48 well plate in AT047 and incubated at 370C with 5% CO2 and 95% air. Samples were taken every 24 hrs and evaluated for cell density and morphology. Cell growth and morphology of HiMedia was found comparable to the competitor medium.

Vero cells were seeded in triplicate in a 48 well plate in AT047 and incubated at 370C with 5% CO2 and 95% air. Samples were taken every 24 hrs and evaluated for cell density and morphology. Cell growth and morphology of HiMedia was found comparable to the competitor medium.

19


Description


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SiGMaProduct Code

LOnzaProduct Code

HycLOnEProduct Code

nctc MEdia (POwdEr)

179 nctc 109 Medium AT138 21340 N1140 - - w/ L-Glutamine (Liquid) (Liquid) w/o Sodium bicarbonate

nutriEnt MixturE F-10 HaM (POwdEr)

180 nutrient Mixture F-10 Ham AT024 - N6635 - SH30009 w/ L-Glutamine w/o Sodium bicarbonate

181 nutrient Mixture F-10 Ham AT024A - - - - w/o L-Glutamine and Sodium bicarbonate

182 nutrient Mixture F-10 Ham AT083 - - - - w/ L-Glutamine and 25 mM HEPES buffer w/o Sodium bicarbonate

183 nutrient Mixture F-10 Ham AT184 - - - - w/ 20mM HEPES and L-Glutamine w/o Sodium bicarbonate

nutriEnt MixturE F-10 HaM (Liquid)

184 nutrient Mixture F-10 Ham AL083 - - - - w/ 25 mM HEPES buffer and Sodium bicarbonate w/o L-Glutamine

185 nutrient Mixture F-10 Ham AL083A 12390 - - - w/ 25mM HEPES, L-Glutamine and Sodium bicarbonate

186 nutrient Mixture F-10 Ham AL024 - N6013 - - w/ Sodium bicarbonate w/o L-Glutamine

187 nutrient Mixture F-10 Ham AL024A 11550 N6908 12-618 SH30025 w/ L-Glutamine and Sodium bicarbonate

188 nutrient Mixture F-10 Ham AL184 - - - - w/ 20mM HEPES and Sodium bicarbonate w/o L-Glutamine

Basal Media

20


Description


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SiGMaProduct Code

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HycLOnEProduct Code

189 nutrient Mixture F-10 Ham AL196 - N2147 - - w/ 20mM HEPES w/o L-Glutamine and Sodium bicarbonate

nutriEnt MixturE F-12 HaM (POwdEr)

190 nutrient Mixture F-12 Ham AT025 21700 N6760 - SH30010 w/ L-Glutamine w/o Sodium bicarbonate

191 nutrient Mixture F-12 Ham AT025A - - - SH30056 w/o L-Glutamine and Sodium bicarbonate

192 nutrient Mixture F-12 Ham AT144 - - - - w/ L-Glutamine w/o Phenol red and Sodium bicarbonate

193 nutrient Mixture F-12 Ham AT085 - - - - w/ L-Glutamine and 25 mM HEPES buffer w/o Sodium bicarbonate

194 nutrient Mixture F-12 Ham AT095 - - - - w/ L-Glutamine w/o Folic acid, Phenol red and Sodium bicarbonate

195 nutrient Mixture F-12 Ham AT119A - - - - w/o Folic acid, L-Glutamine, Phenol red and Sodium bicarbonate

196 nutrient Mixture F-12 Ham, kaighn’s Modification AT106 - N3520 - - w/ L-Glutamine w/o Sodium bicarbonate

197 nutrient Mixture F-12 Ham, kaighn’s Modification AT106A - - - - w/ L-Glutamine and Sodium bicarbonate

198 nutrient Mixture F12 Ham AT144A - - - - w/o L-Glutamine, Phenol red and Sodium bicarbonate

199 nutrient Mixture F12 Ham, coon’s Modification AT146 - F6636 - - w/L-Glutamine w/o Sodium bicarbonate

200 nutrient Mixture F-12 Ham, coon’s Modification AT146A - - - - w/o L-Glutamine and Sodium bicarbonate

Basal Media

21


Description


Sr.No.


SiGMaProduct Code

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HycLOnEProduct Code

nutriEnt MixturE F-12 HaM (Liquid)

201 nutrient Mixture F-12 Ham AL025 - N4888 - - w/ Sodium bicarbonate w/o L-Glutamine

202 nutrient Mixture F-12 Ham AL025A 11765 N6658 12-615 SH30026 w/ L-Glutamine and Sodium bicarbonate

203 nutrient Mixture F-12 Ham AL025Sv - - - - w/ 2mM L-Glutamine and 1.5 gms per litre Sodium bicarbonate

204 nutrient Mixture F-12 Ham AL085 - N8641 - - w/ 25mM HEPES buffer and Sodium bicarbonate w/o L-Glutamine

205 nutrient Mixture F-12 Ham, kaighn’s Modification AL106 - - - - w/ Sodium bicarbonate w/o L-Glutamine

206 nutrient Mixture F-12 Ham, kaighn’s Modification AL106A 21127 SH30526 w/ L-Glutamine and Sodium bicarbonate

207 nutrient Mixture F-12 Ham, kaighn’s Modification AL106Sv - - - - w/ L-Glutamine and 1.5 gms per litre Sodium bicarbonate

208 nutrient Mixture F-12 Ham, coon’s Modification AL146 - - - - w/ Sodium bicarbonate w/o L-Glutamine

Basal Media


comparative analysis of performance of nutrient mixture F-12 Ham using cHO cell line


22


Description


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LOnzaProduct Code

HycLOnEProduct Code

rPMi-1640 (POwdEr)

209 rPMi-1640 AT150 - R1383 - - w/ L-Glutamine w/o Glucose and Sodium bicarbonate

210 rPMi-1640 AT028 31800 R6504 15-702 SH30011 w/ L-Glutamine w/o Sodium bicarbonate

211 rPMi-1640 AT028A - - - SH30057 w/o L-Glutamine and Sodium bicarbonate

212 rPMi-1640 AT028F - - - - w/ L-Glutamine w/o Folic acid and Sodium bicarbonate

213 rPMi-1640 AT161A - - - - w/o Folic acid, L-Glutamine and Sodium bicarbonate

214 rPMi-1640 AT112A - - - - w/o L-Glutamine, Phenol red and Sodium bicarbonate

215 rPMi-1640 AT120 - R8755 - SH30197 w/ L-Glutamine w/o Phenol red and Sodium bicarbonate.

216 rPMi-1640 AT060 23400 R4130 - - w/ L-Glutamine and 25mM HEPES buffer w/o Sodium bicarbonate

217 rPMi-1640 AT060A - - - - w/ 25mM HEPES buffer w/o L-Glutamine and Sodium bicarbonate

218 rPMi- 1640 AT157A - - - - w/ HEPES buffer and Sodium pyruvate w/o L-Glutamine and Sodium bicarbonate

219 rPMi-1640 AT129 - - - - w/ L-Glutamine and 25mM HEPES buffer w/o L-Arginine and Sodium bicarbonate

220 rPMi-1640 AT113A - - - - w/ 25mM HEPES buffer w/o L-Glutamine, Phenol red and Sodium bicarbonate

Basal Media

23


Description


Sr.No.


SiGMaProduct Code

LOnzaProduct Code

HycLOnEProduct Code

221 rPMi- 1640 AT162 - - - - w/ L-Glutamine, Sodium pyruvate, 4.5 gms Glucose per litre and 10mM HEPES buffer w/o Sodium bicarbonate

222 rPMi- 1640 AT171 - - - - w/ 2mM L-Glutamine, 1mM Sodium pyruvate, 4.5 gms Glucose per litre and 10mM HEPES buffer w/o Sodium bicarbonate and Phenol red

223 rPMi-1640 (Modified for autoclaving) AT126A - R7755 - SH30328 w/o L-Glutamine and Sodium bicarbonate

224 rPMi-1640 AT181 - - - - w/ L-Glutamine w/o Folic acid, Phenol red and Sodium bicarbonate

225 rPMi-1640 AT180 - - - - w/ L-Glutamine, Phenol red, 0.2% Glucose and 0.165 moles per litre MOPS buffer w/o Sodium bicarbonate

rPMi-1640 (Liquid)

226 rPMi-1640 AL028 21870 R0883 12-167 SH30096 w/ Sodium bicarbonate w/o L-Glutamine

227 rPMi-1640 AL028B - - - - w/ Sodium bicarbonate w/o L-Glutamine Hybridoma tested 228 rPMi-1640 AL028A 11875 R8758 12-702 SH30027 w/ L-Glutamine and Sodium bicarbonate

229 rPMi-1640 AL120A 11835 - - - w/ L-Glutamine and Sodium bicarbonate w/o Phenol red

230 rPMi-1640 AL028F - - - - w/ Sodium bicarbonate w/o Folic acid and L-Glutamine

231 rPMi-1640 AL162A - - - - w/ 1mM Sodium pyruvate, 2mM L-Glutamine, 4.5 gms Glucose per litre, 10mM HEPES and 2 gms per litre Sodium bicarbonate

Basal Media

24


Description


Sr.No.


SiGMaProduct Code

LOnzaProduct Code

HycLOnEProduct Code

232 rPMi-1640 AL171A - - - - w/ 1mM Sodium pyruvate, 2mM L-Glutamine, 4.5 gms Glucose per litre, 10mM HEPES and Sodium bicarbonate w/o Phenol red

233 rPMi-1640 AL162Sv A10491 - - - w/ 1mM Sodium pyruvate, 2mM L-glutamine, 4.5 gms Glucose per litre, 10 mM HEPES and 1.5 gms per litre Sodium bicarbonate

234 rPMi-1640 AL157 - - - - w/ 25mM HEPES buffer, Sodium bicarbonate and Sodium pyruvate w/o L-Glutamine

235 rPMi-1640 AL060 - R5886 04-558 SH30203 w/ Sodium bicarbonate and 25mM HEPES buffer w/o L-Glutamine

236 rPMi-1640 AL060A 22400 - 12-115 SH30255 w/ L-Glutamine, 25mM HEPES buffer and Sodium bicarbonate

237 rPMi-1640, 10x AL102 - - - - w/o Sodium bicarbonate and L-Glutamine

238 rPMi-1640 AL180A - - - - w/ L-Glutamine, 2 gms Glucose per litre, 0.165 Moles per litre, MOPS Buffer and Sodium bicarbonate

239 rPMi-1640 AL200A - - 04-525 - w/ L-Glutamine, 2 gms Glucose per litre, 0.165 Moles per litre and MOPS Buffer w/o Sodium bicarbonate

240 rPMi-1640 AL181A - - - - w/ L-Glutamine and Sodium bicarbonate w/o Folic acid and Phenol red

241 rPMi-1640 AL197A - R7388 - - w/ L-Glutamine and 20mM HEPES Buffer w/o Sodium bicarbonate

242 rPMi-1640, Dutch Modification AL198 22409 R7638 - - w/ 20mM HEPES Buffer and 1 gms per litre Sodium bicarbonate w/o L-Glutamine

Basal Media


25


Description


Sr.No.


SiGMaProduct Code

LOnzaProduct Code

HycLOnEProduct Code

243 rPMi-1640 AL199S - - - - w/ 1mM Sodium pyruvate, 2mM L-Glutamine, 4.5 gms Glucose per litre and 1.5 gms per litre Sodium bicarbonate w/o HEPES buffer

244 rPMi-1640 AL201A - - - - w/ 25mM HEPES Buffer, L-Glutamine and 2.2gms per litre Sodium bicarbonate

Basal Media

Jurkat cells were seeded in triplicate in a 48 well plate in AT028 and incubated at 370C with 5% CO2 and 95% air. Samples were taken every 24 hrs and evaluated for cell density and morphology. Cell growth and morphology of HiMedia was found comparable to the competitor medium.


comparative analysis of performance of HiMedia rPMi-1640 vs competitor using jurkat and cHO cell line

26


Description


Sr.No.


SiGMaProduct Code

LOnzaProduct Code

HycLOnEProduct Code

SFrE MEdiuM 199 (Powder)

245 SFrE Medium 199-1 AT089 - - - - w/ Hank’s salts, L-Glutamine, Galactose and Glucose w/o Sodium bicarbonate and Insulin

246 SFrE Medium 199-2 AT090 - - - - w/ Earle’s salts, L-Glutamine, Galactose and Glucose w/o Sodium bicarbonate and Insulin

SwiM’S S-77 MEdiuM (Powder)

247 Swim’s S-77 Medium (Powder) AT124 - - - - w/ L-Glutamine w/o Streptomycin sulphate, Phenol red and Sodium bicarbonate

wayMOutH MEdiuM MB 752/1 (Powder)

248 waymouth Medium MB 752/1 (Powder) AT091 - W1625 - - w/ L-Glutamine w/o Sodium bicarbonate

wayMOutH MEdiuM MB 752/1 (Liquid)

249 waymouth Medium MB 752/1 AL091A 11220 - BE12-738 - w/ L-Glutamine and Sodium bicarbonate

wiLLiaM’S MEdiuM E (Powder)

250 william’s Medium E AT125 - W4125 - SH30094 w/ L-Glutamine w/o Sodium bicarbonate

wiLLiaM’S MEdiuM E (Liquid)

251 william’s Medium E AL125 12551 W4128 12-761 - w/ Sodium bicarbonate w/o L-Glutamine

Basal Media

27


Description


Sr.No.


SiGMaProduct Code

LOnzaProduct Code

HycLOnEProduct Code

inSEct MEdia (POwdEr)

252 Grace’s insect Medium IM001 11300 G9771 - - w/ L-Glutamine w/o Sodium bicarbonate

253 Mitsuhashi and Maramorosch insect Medium IM002 - - - - w/o Sodium bicarbonate

254 Schneider’s insect Medium IM003 - S9895 - - w/ L-Glutamine w/o Calcium chloride and Sodium bicarbonate

255 Sheilds and Sang M3 insect Medium IM004 - S8398 - - w/ L-Glutamine w/o Potassium bicarbonate

256 d-22 insect Medium IM005 - - - - w/o Sodium bicarbonate

257 iPL-41 insect Medium IM006 - - - - w/ L-Glutamine w/o Calcium chloride, Sodium bicarbonate and Tryptose Phospahte Broth

258 tc-100 insect Medium IM007 - - - - w/ L-Glutamine w/o Sodium bicarbonate

259 tnM-FH insect Medium IM008 - T1032 - - w/ L-Glutamine, Lactalbumin hydrolysate and Yeast extract w/o Sodium bicarbonate

inSEct MEdia (Liquid)

260 Graces insect Medium IML001 - - - SH30610 w/ Sodium bicarbonate w/o L-Glutamine

261 Grace’s insect Medium IML001A 11595 G8142 04-457 - w/ L-Glutamine and Sodium bicarbonate

262 Mitsuhashi and Maramorosch insect Medium IML002 - - - - w/ Sodium bicarbonate

263 Schneider’s insect Medium IML003 - - - - w/ Calcium chloride and Sodium bicarbonate w/o L-Glutamine

Insect Media

28


Description


Sr.No.


SiGMaProduct Code

LOnzaProduct Code

HycLOnEProduct Code

264 Schneider’s insect Medium IML003A 21720 S0146 04-351 - w/ Calcium Chloride, L-Glutamine and Sodium bicarbonate

265 Sheilds and Sangs M3 insect Medium IML004 - S3652* - - w/ Potassium bicarbonate w/o L-Glutamine

266 d-22 Medium IML005 - - - -

267 iPL-41 insect Medium IML006 11405* I7760* - - w/ Calcium chloride and Sodium bicarbonate w/o L-Glutamine

268 tc-100 insect Medium IML007 - T3160* 02-011* - w/ Sodium bicarbonate w/o L-Glutamine

269 tnM-FH insect Medium IML008 - - - SH30280 w/ Lactalbumin hydrolysate, Yeast extract and Sodium bicarbonate w/o L-Glutamine

270 tnM-FH insect Medium IML008A 11605 T3285 - - w/ L-Glutamine, Lactalbumin hydrolysate, Yeast extract and Sodium bicarbonate

inSEct diEtS

271 drosophila diet ID001 - - - -

Insect Media

*I7760 : with Glutamine *T3160 : with L-Glutamine *11405 : with Glutamine*S3652 : with Glutamine *02-011 : with L-Glutamine

comparative analysis of performance of HiMedia Grace’s and Schneider’s insect medium vs competitor using Sf9 & dmel cell line

Dmel cells were seeded in triplicate in a 48 well plate in IM003 and incubated at 370C with 5% CO2 and 95% air. Samples were taken every 24 hrs and evaluated for cell density and morphology. Cell growth and morphology of HiMedia was found comparable to the competitor medium.

Sf9 cells were seeded in triplicate in a 48 well plate in IM001 and incubated at 370C with 5% CO2 and 95% air. Samples were taken every 24 hrs and evaluated for cell density and morphology. Cell growth and morphology of HiMedia was found comparable to the competitor medium.

29


Description


Sr.No.


SiGMaProduct Code

LOnzaProduct Code

HycLOnEProduct Code

HiGlutaxLtM MEdia (MEdia cOntaininG L-aLanyL-L-GLutaMinE)

272 HiGlutaxLtM dulbecco’s Modified Eagle Medium, High Glucose AL007G 10569 D0822 12-604/U1 - w/ L-Alanyl-L-Glutamine, Sodium pyruvate and Sodium bicarbonate 273 HiGlutaxLtM dulbecco’s Modified Eagle Medium, High Glucose AL066G 10566 D0819 - - w/ 4.5gms per litre Glucose, L-Alanyl L-Glutamine and Sodium bicarbonate w/o Sodium pyruvate 274 HiGlutaxLtM dulbecco’s Modified Eagle Medium / nutrient Mixture F-12 Ham (dMEM / F12, 1:1 Mixture) AL127G - - - - w/ L-Alanyl-L-Glutamine, Sodium bicarbonate and Trace elements w/o HEPES buffer 275 HiGlutaxLtM dulbecco’s Modified Eagle Medium / nutrient Mixture F-12 Ham (dMEM / F12, 1:1 Mixture) AL139G - - - - w/L-Alanyl-L-Glutamine, HEPES buffer, Sodium bicarbonate and Trace elements 276 HiGlutaxLtM iscove’s Modified dulbecco’s Medium AL070G 31980 - - - w/ L-Alanyl-L-Glutamine, HEPES buffer and Sodium bicarbonate 277 HiGlutaxLtM Leibovitz’s L-15 Medium AL011G - - - - w/ L-Alanyl-L-Glutamine and Sodium bicarbonate 278 HiGlutaxLtM Minimum Essential Medium Eagle AL047G - - - - w/ L-Alanyl-L-Glutamine, Earle’s salts, NEAA and Sodium bicarbonate 279 HiGlutaxLtM Minimum Essential Medium Eagle AL020G 41090 M0446 - - w/ L-Alanyl-L-Glutamine, Earle’s salts and Sodium bicarbonate w/o NEAA 280 HiGlutaxLtM Minimum Essential Medium Eagle AL056G - - - - w/ L-Alanyl-L-Glutamine, Hank’s salts and Sodium bicarbonate 281 HiGlutaxLtM Minimum Essential Medium (alpha Modification) AL080G 32571 M6199 02-002 - w/ L-Alanyl-L-Glutamine, Deoxyribonucleosides, Ribonucleosides and Sodium bicarbonate 282 HiGlutaxLtM Minimum Essential Medium (alpha Modification) AL081G 32561 - - - w/ L-Alanyl-L-Glutamine and Sodium bicarbonate w/o Deoxyribonucleosides and Ribonucleosides 283 HiGlutaxLtM nutrient Mixture F-10 Ham AL024G - - 02-014F - w/ L-Alanyl-L-Glutamine and Sodium bicarbonate 284 HiGlutaxLtM nutrient Mixture F-12 Ham AL025G 31765 N3790 - - w/ L-Alanyl-L-Glutamine and Sodium bicarbonate 285 HiGlutaxLtM rPMi-1640 AL028G 61870 R2405 12-702/U1 - w/ L-Alanyl-L-Glutamine and Sodium bicarbonate 286 HiGlutaxLtM rPMi-1640 AL060G 72400 - 12-115/U1 - w/ L-Alanyl-L-Glutamine, HEPES buffer and Sodium bicarbonate

HiGluta XLTM Media

30


Description


Sr.No.


SiGMaProduct Code

LOnzaProduct Code

HycLOnEProduct Code

rEducEd SEruM MEdia

287 LoSeratM dulbecco’s Modified Eagle Medium, High Glucose RSL003 12491 M3942 - SH30565* w/ Sodium pyruvate and Sodium bicarbonate w/o L-Glutamine

288 LoSeratM dulbecco’s Modified Eagle Medium, High Glucose RSL003G - - - - w/ L-Alanyl-L-Glutamine, Sodium pyruvate and Sodium bicarbonate

289 LoSeratM dulbecco’s Modified Eagle Medium / nutrient Mixture F-12 Ham (1:1) RSL005 12634 M4192 - - w/ Trace elements and Sodium bicarbonate w/o L-Glutamine and HEPES buffer

290 LoSeratM dulbecco’s Modified Eagle Medium / nutrient Mixture F-12 Ham (1:1) RSL006 - - - - w/ HEPES buffer, Sodium bicarbonate and Trace elements w/o L-Glutamine

291 LoSeratM dulbecco’s Modified Eagle Medium / nutrient Mixture F-12 Ham (1:1) RSL006G - - - - w/ L-Alanyl-L-Glutamine, HEPES buffer, Sodium bicarbonate and Trace elements

292 LoSeratM iscove’s Modified dulbecco’s Medium RSL007 - - - - w/ HEPES buffer and Sodium bicarbonate w/o L-Glutamine

293 LoSeratM Minimum Essential Medium Eagle RSL008 12492 - 12-745 SH30564* w/ Earle’s salts, NEAA and Sodium bicarbonate w/o L-Glutamine

294 LoSeratM Minimum Essential Medium Eagle RSL008G 51985 - - - w/ Earle’s salts, L-Alanyl-L-Glutamine, NEAA and Sodium bicarbonate 295 LoSeratM nutrient Mixture F-10 Ham RSL009 - - - - w/ Sodium bicarbonate w/o L-Glutamine 296 LoSeratM nutrient Mixture F-12 Ham RSL010 - - - SH30623* w/ Sodium bicarbonate w/o L-Glutamine 297 LoSeratM rPMi-1640 RSL011 12633 M3817 - - w/ Sodium bicarbonate w/o L-Glutamine 298 LoSeratM rPMi-1640 RSL011G - - - - w/ L-Alanyl-L-Glutamine and Sodium bicarbonate 299 LoSeratM rPMi-1640 RSL012 - - - - w/ HEPES buffer and Sodium bicarbonate w/o L-Glutamine

LoSeraTM Media

*SH30564 : with Glutamine*SH30623 : with Glutamine *SH30565 : with L-Glutamine

31


Description


Sr.No.


SiGMaProduct Code

LOnzaProduct Code

HycLOnEProduct Code

cELL FrEEzinG MEdia

300 cell Freezing Medium - dMSO, 1x TCL043 12648 C6164 - -

301 cell Freezing Medium - Glycerol, 1x TCL044 - C6039 - -

302 cell Freezing Medium - dMSO, 1x without Phenol red TCL056 - - - -

303 cell Freezing Medium - Glycerol, 1x without Phenol red TCL078 - - - -

304 cell Freezing Medium - dMSO, 1x TCL093 - - - - This medium contains high concentration of FBS and can be used for freezing of sensitive cell lines

305 Serum Free cell Freezing Medium - dMSO, 1x TCL082 A12542 C6295 - -

cytOGEnEticS rELatEd PrOductS

306 HikaryoxLtM rPMi Medium AL165A 12557 - BE02-015E - w/ L-Glutamine, FBS, PHA-M, Penicillin, Streptomycin and Sodium bicarbonate

307 HikaryoxLtM nutrient Mixture F10 Medium AL169A - - - - w/ L-Glutamine, FBS, PHA-M, Penicillin, Streptomycin and Sodium bicarbonate

308 HikaryoxLtM rPMi Medium AL173A - - - - w/ L-Glutamine, FBS, Penicillin, Streptomycin and Sodium bicarbonate w/o PHA-M

309 HikaryoxLtM nutrient Mixture F10 Medium AL185A - - - - w/ L-Glutamine, FBS, Penicillin, Streptomycin and Sodium bicarbonate w/o PHA-M

310 HikaryoxLtM colchicine Solution TCL062 - - - - w/ 10µg per ml Colchicine in Phosphate Buffered Saline

311 HikaryoxLtM PHa-M Solution TCL061 - - - - w/ 0.1 mg per ml, PHA-M in sterile tissue culture grade water

312 HikaryoxLtM PHa-M Solution TCL071 - - - - w/ 1 mg per ml, PHA-M in sterile tissue culture grade water

313 HikaryoxLtM colcemid Solution TCL074 15210 D1925 - - w/ 10 µg per ml Colcemid in Hank’s Balanced Salt Solution

314 Potassium chloride solution 0.075 M TCL040 10575 P9327 - -

315 Giemsa Stain Solution TCL083 10092 - - -

Cell Freezing Media & Cytogenetics

32


Description


Sr.No.


SiGMaProduct Code

LOnzaProduct Code

HycLOnEProduct Code

vitaMin SOLutiOnS (StEriLE FiLtErEd)

316 BME vitamin Solution 100x VA001 78-5801 B6891 - -

317 MEM vitamin Solution 100x VA002 11120 M6895 13-607 SH30599

318 rPMi 1640 vitamin Solution 100x VA003 - R7256 - -

aMinO acid cOncEntratE (POwdEr)

319 Basal Medium Eagle (BME) amino acids 50x AC001 - - - - w/o L-Glutamine

320 MEM amino acids 50x AC002 - - - - w/o L-Glutamine

321 MEM dulbecco’s Modification amino acids 50x AC004 - - - -

322 MEM Glasgow Modification, amino acids 50x AC003 - - - - w/o L-Glutamine

323 MEM-non Essential amino acids (nEaa) 50x AC005 - - - -

aMinO acid cOncEntratE (Liquid)

324 MEM amino acids Solution 100x ACL002 11130 M5550 - SH30598 w/o L-Glutamine (50x) (50x) (50x)

325 MEM Glasgow’s Modification amino acids Solution 100x ACL003 - - - - w/o L-Glutamine

326 MEM non Essential amino acids Solution 50x ACL005 - - - -

327 MEM non Essential amino acids Solution 100x ACL006 11140 M7145 13-114 SH30238

Vitamin Solution and Amino Acid Concentrates

33


Description


Sr.No.


SiGMaProduct Code

LOnzaProduct Code

HycLOnEProduct Code

BaLancEd SaLtS (POwdEr)

328 dulbecco’s Phosphate Buffered Saline TS1119 21300 - - - w/ Calcium and Magnesium

329 dulbecco’s Phosphate Buffered Saline TS1006 21600 D5652 15-456 SH30013 w/o Calcium and Magnesium

330 dulbecco’s Phosphate Buffered Saline TS1121 - D5773 - - w/ Magnesium w/o Calcium

331 Earle’s Balanced Salts TS1002 - - BE10-502F SH30014 w/ Phenol red w/o Sodium bicarbonate

332 Earle’s Balanced Salts TS1009 - - - - w/o Phenol red and Sodium bicarbonate

333 Hank’s Balanced Salts TS1003 - H6136 - SH30015 w/ Phenol red w/o Sodium bicarbonate

334 Hank’s Balanced Salts TS1010 - H1387 - - w/o Phenol red and Sodium bicarbonate

335 Hank’s Balanced Salts TS1033 - H2387 - SH30016 w/ Phenol red w/o Calcium, Magnesium and Sodium bicarbonate

336 Hank’s Balanced Salts TS1020 - - - - w/ Phenol red w/o Calcium and Sodium bicarbonate

337 Hank’s Balanced Salts TS1098 - H4891 - SH30107 w/o Calcium, Magnesium, Phenol red and Sodium bicarbonate

338 krebs-ringer Bicarbonate Buffer TS1097 - K4002 - - w/ 1.8 gms Glucose per litre w/o Calcium chloride and Sodium bicarbonate

339 Spinner Salts, Eagle TS1011 - - - - w/ Phenol red w/o Calcium chloride and Sodium bicarbonate

340 tyrode’s Salts TS1012 - T2145 - - w/o Phenol red and Sodium bicarbonate

Balanced Salt

34


Description


Sr.No.


SiGMaProduct Code

LOnzaProduct Code

HycLOnEProduct Code

341 wesson’s Salt Mixture TS1100 - - - - Insect Cell Culture Tested

342 Phosphate Buffered Saline, pH 7.2 TS1099 - - - -

343 Phosphate Buffered Saline pH 7.4 TS1101 - P3813 - -

344 Phosphate Buffered Saline pH 7.4 TS1120 - P3688 - - w/ 1% Bovine Serum Albumin

BaLancEd SaLt SOLutiOnS (Liquid)

345 dulbecco’s Phosphate Buffered Saline 1x TL1006 14190 D8537 17-512 SH30028 w/o Calcium and Magnesium

346 dulbecco’s Phosphate Buffered Saline 1x TL1023 14040 D8662 17-513 SH30264 w/ Calcium and Magnesium

347 dulbecco’s Phosphate Buffered Saline 1x TL1106 14287 - 04-479 - w/ 1000 mg per litre D-Glucose and 36 mg per litre Sodium pyruvate, Calcium and Magnesium w/o Phenol red

348 dulbecco’s Phosphate Buffered Saline 10x TL1022 14200 D1408 17-515 SH30378 w/o Calcium and Magnesium

349 dulbecco’s Phosphate Buffered Saline 5x TL1028 - - - - w/ Calcium and Magnesium

350 dulbecco’s Phosphate Buffered Saline 10x TL1029 14080 D1283 - SH30597 w/ Calcium and Magnesium

351 Earle’s Balanced Salt Solution 1x TL1002 - E2888 BE10-502F SH30029 w/ Phenol red and Sodium bicarbonate

352 Earle’s Balanced Salt Solution 1x TL1009 - E3024 - - w/ Sodium bicarbonate w/o Phenol red

353 Earle’s Balanced Salt Solution 10x TL1020 - E7510 - - w/ Phenol red w/o Sodium bicarbonate

354 Earle’s Balanced Salt Solution 1x TL1107 14155 - - - w/ Sodium bicarbonate w/o Calcium, Magnesium and Phenol red

355 Earle’s Balanced Salt Solution 1x TL1110 - E6267 - - w/ Sodium bicarbonate and Phenol red w/o Calcium and Magnesium

Balanced Salt

35


Description


Sr.No.


SiGMaProduct Code

LOnzaProduct Code

HycLOnEProduct Code

356 Hank’s Balanced Salts Solution 1x TL1024 - - - - w/ Phenol red and Sodium bicarbonate w/o Calcium

357 Hank’s Balanced Salt Solution 1x TL1003 24020 H9269 10-508 SH30030 w/ Phenol red and Sodium bicarbonate

358 Hank’s Balanced Salt Solution 1x TL1010 14025 H8264 10-527 SH30268 w/ Sodium bicarbonate w/o Phenol red

359 Hank’s Balanced Salt Solution 1x TL1019 - - - - w/ Phenol red w/o Sodium bicarbonate

360 Hank’s Balanced Salt Solution 10X TL1021 - H1641 - - w/ Phenol red w/o Sodium bicarbonate

361 Hank’s Balanced Salt Solution 1X TL1108 - - - - w/ Phenol red w/o Calcium, Magnesium and Sodium bicarbonate

362 Hank’s Balanced Salt Solution 10x TL1109 14065 - - - w/ Calcium and Magnesium w/o Sodium bicarbonate and Phenol red

363 Hank’s Balanced Salt Solution 10X TL1122 - H4641 - - w/ Phenol red w/o Calcium, Magnesium and Sodium bicarbonate

364 Hank’s Balanced Salt Solution 1x TL1034 14170 H9394 10-543 SH30031 w/ Phenol red and Sodium bicarbonate w/o Calcium and Magnesium

365 Hank’s Balanced Salt Solution 1x TL1098 14175 H6648 10-547 SH30588 w/ Sodium bicarbonate w/o Calcium, Magnesium and Phenol red

366 krebs - ringer Bicarbonate Buffer 1x TL1097 - - - - w/ 1.8 gms Glucose per liter and Sodium bicarbonate w/o Calcium chloride

367 Phosphate Buffered Saline, pH 7.2 1x TL1031 20012 - - SH30256

368 Phosphate Buffered Saline, pH 7.2 10x TL1032 70013 - - SH30258

369 Phosphate Buffered Saline, pH 7.2 20x TL1033 - - - -

370 Phosphate Buffered Saline, pH 7.4 1x TL1101 10010 - 17-516 -

Balanced Salt

36


Description


Sr.No.


SiGMaProduct Code

LOnzaProduct Code

HycLOnEProduct Code

371 Phosphate Buffered Saline, pH 7.4 10x TL1099 70011 P5493 17-517 -

372 Potassium Phosphate Buffer, pH 8.0 1x TL1100 - - - -



375 Sodium Phosphate Buffered Saline, 1x TL1104 - - - -

376 Spinner Balanced Salts, Eagle 1x TL1011 - - - - w/ Phenol red and Sodium bicarbonate w/o Calcium chloride

377 tyrode’s Balanced Salts 1x TL1012 - T2397 - - w/ Sodium bicarbonate w/o Phenol red

Balanced Salt

37


Description


Sr.No.


SiGMaProduct Code

LOnzaProduct Code

HycLOnEProduct Code

cELL diSSOciatiOn rEaGEntS

378 accutasetM TCL075 A11105 A6964 - SV30030

379 trypsin 0.25% Solution 1x TCL006 - - - - 0.25% Trypsin in Dulbecco’s Phosphate Buffered Saline Sterile filtered

380 trypsin 0.1% Solution 1x TCL011 - - - - 0.1% Trypsin in Dulbecco’s Phosphate Buffered Saline Sterile filtered

381 trypsin 0.25% Solution 1x TCL047 15050 T4424 - - 0.25% Trypsin in Hank’s Balanced Salt Solution with Phenol red w/o Calcium and Magnesium

382 trypsin 2.5% Solution 10x TCL008 - - 17-160 SV30037 2.5% Trypsin in Hank’s Balanced Salt Solution 10X w/o Phenol red

383 trypsin 2.5% Solution, 10x TCL051 - T4674 - - 2.5% Trypsin in Hank’s Balanced Salt Solution w/ Phenol red

384 trypsin 2.5% Solution, 10x TCL032 15090 T4549 - - 2.5% Trypsin in 0.9% normal saline

385 trypsin - Edta Solution 1x TCL007 - - - - 0.25% Trypsin and 0.02% EDTA in Dulbecco’s Phosphate Buffered Saline

386 trypsin - Edta Solution 1x TCL049 - T4049 - - 0.25% Trypsin and 0.02% EDTA in Hank’s Balanced Salt Solution w/ Phenol red

387 trypsin - Edta Solution 1x TCL048 25200 - - - 0.25% Trypsin, 0.038% EDTA in Hank’s Balanced Salt Solution and Phenol Red w/o Calcium and Magnesium

388 trypsin - Edta Solution 1x TCL014 - - - - 0.25% Trypsin and 0.001% EDTA in Dulbecco’s Phosphate Buffered Saline

389 trypsin-Edta Solution 1x TCL033 - T3924 - - 0.05% Trypsin, 0.02% EDTA in Hank’s Balanced Salt Solution w/ Phenol red

390 trypsin-Edta Solution 1x TCL050 25300 - - SH30236 0.05% Trypsin(1:250) Gamma irradiated, 0.02% EDTA in Hank’s Balanced Salt Solution w/ Phenol red

Cell Dissociation Reagents

38


Description


Sr.No.


SiGMaProduct Code

LOnzaProduct Code

HycLOnEProduct Code

391 trypsin-Edta Solution 1x TCL042 - - - - w/ 0.25% Trypsin, 0.1% EDTA in Hank’s Balanced Salt Solution w/o Phenol red

392 trypsin - Edta Solution 1x T001 - - - - 0.25% Trypsin and 0.2% EDTA in Dulbecco’s Phosphate Buffered Saline Sterile filtered

393 trypsin-Edta Solution 10x TCL034 15400 T4174 02-007 - 0.5% Trypsin, 0.2% EDTA in 0.85% normal saline w/o Phenol red

394 trypsin-Edta Solution 10x TCL070 - - - - w/ 2.5% Trypsin (1:250), 0.2% EDTA in 0.85% normal Saline w/o Calcium and Magnesium

395 trypsin-Edta Solution 1x TCL081 - T4299 - - w/ 500BAEE units Porcine trypsin and 180µg per ml EDTA tetra sodium salt in Dulbecco’s Phosphate Buffered Saline

396 trypsin Phosphate versene Glucose (tPvG) Solution 1x TCL022 - - - - 0.25% Trypsin, 0.5% PVP, 0.02% EDTA and 0.05% Glucose in Dulbecco’s Phosphate Buffered Saline w/o Calcium and Magnesium

397 trypsin Phosphate versene Glucose (tPvG) Solution 1x TCL031 - - - - 0.1% Trypsin, 0.02% EDTA, 0.05% Glucose and Phenol red in Dulbecco’s Phosphate Buffered Saline w/o Calcium and Magnesium

398 versene (Edta) 0.1% Solution 1x TCL020 - - - - 0.1% Versene in Dulbecco’s Phosphate Buffered Saline

399 versene (Edta) 0.02% Solution 1x TCL053 15040 E8008 17-711 - 0.02% Versene in Dulbecco’s Phosphate Buffered Saline

EnzyME FrEE cELL diSSOciatiOn rEaGEntS

400 zymeFree™Enzyme Free cell dissociation reagent in dulbecco’s Phosphate Buffered Saline 1x TCL028 13151 C5914 - -

401 zymeFree™ Enzyme Free cell dissociation reagent in Hank’s Balanced Salt Solution 1x TCL029 13150 C1419/C5789 - -

Cell Dissociation Reagents

39


Description


Sr.No.


SiGMaProduct Code

LOnzaProduct Code

HycLOnEProduct Code

antiBiOtic SOLutiOnS (StEriLE FiLtErEd)

402 amphotericin B Solution 250µg/ml A011 15290 A2942 17-836 SV30078

403 ampicillin solution A022 - - - - 10mg/ml Ampicillin sodium salt in sterile tissue culture grade water

404 antibiotic Solution 100x liquid A001 15140 P0781 17-602 SV30010 w/ 10,000 U Penicillin and 10 mg Streptomycin per ml in 0.9% normal saline

405 ampicillin solution A023 - A5354 - - 100mg/ml Ampicillin sodium salt in sterile tissue culture grade water

406 antibiotic Solution 100x liquid, Endotoxin tested A001A - - - - w/ 10,000 U Penicillin and 10 mg Streptomycin per ml in 0.9% normal saline

407 antibiotic Solution 100x Liquid A014 - P4333 - - w/ 10,000U Penicillin and 10 mg Streptomycin per ml in citrate buffer

408 antibiotic Solution 100x liquid A018 - - - - w/ 10,000U Penicillin, 5mg Streptomycin per ml in 0.9% normal saline

409 antibiotic antimycotic Solution 100x liquid A002 15240 A5955 17-745 SV30079 w/10,000 U Penicillin, 10mg Streptomycin and 25 µg Amphotericin B per ml in 0.9% normal saline

410 antibiotic antimycotic Solution 100x liquid, Endotoxin tested A002A - - - - w/ 10,000 U Penicillin, 10mg Streptomycin and 25 µg Amphotericin B per ml in 0.9% normal saline

411 antibiotic Solution 50x liquid A003 15070 - 17-603 - w/ 5,000 U Penicillin and 5 mg/ml Streptomycin in 0.9% normal saline

412 carbenicillin Solution 100mg/ml A019 - - - - w/100 mg/ml Carbenicillin in sterile tissue culture grade water

413 cephotaxime Solution 250mg/ml A025 - - - - w/ 250mg/ml Cephotaxime in sterile tissue culture grade water

414 cephotaxime Solution 100mg/ml A020 - - - - w/100 mg/ml Cephotaxime in sterile tissue culture grade water

415 Geneticin Solution A016 10131 G8168 - SV30069 w/ 50 mg/ml Geneticin in sterile tissue culture grade water

416 Gentamicin Solution A005 15750 G1397 17-518 SV30080 w/ 50 mg/ml Gentamicin in sterile tissue culture grade water

417 Gentamicin L-Glutamine Solution A006 - G9654 - - w/ 200mM L-Glutamine and 5 mg/ml Gentamicin in 0.9% normal saline

418 Gentamicin Solution A010 15710 G1272 17-519 - w/ 10mg/ml Gentamicin sulfate in sterile tissue culture grade water

Antibiotic Solution

40


Description


Sr.No.


SiGMaProduct Code

LOnzaProduct Code

HycLOnEProduct Code

419 Hygromycin B Solution A015 10687 H0654 - SV30070 w/ 50 mg/ml Hygromycin B in sterile tissue culture grade water

420 kanamycin Solution A008 15160 K0129 - - w/ 10 mg/ml Kanamycin in 0.9% normal saline Sterile filtered

421 kanamycin Solution A009 - K0254 - - w/ 50 mg/ml Kanamycin in 0.9% normal saline

422 neomycin sulphate Solution A024 - N1142 - - w/ 10mg/ml Neomycin in 0.9% normal saline

423 nystatin Solution A012 - N1638 - - w/ 10,000units Nystatin per ml in Dulbecco’s Phosphate Buffered Saline

424 Polymyxin B Solution A013 - - - - w/ 50 mg/ml Polymyxin B in sterile tissue culture gradeater

425 L-Glutamine-Penicillin-Streptomycin Solution A007 10378 G1146 - SV30082 w/ 200mM L-Glutamine, 10,000 units/ml Penicillin and 10 mg/ml Streptomycin in 0.9% normal saline

426 Penicillin Streptomycin Powder A004 - - - - w/ 5000 units Penicillin and 5 mg Streptomycin per ml in 0.9% normal saline when reconstituted with sterile tissue culture grade water to indicated volume.

Antibiotic Solution

41


Description


Sr.No.


SiGMaProduct Code

LOnzaProduct Code

HycLOnEProduct Code

SEra aduLt BOvinE SEra

427 adult bovine serum RM9981 16170 B9433 14-401 SH30409 Origin : Australia (New Zealand) (US) (US) (New Zealand)

FEtaL BOvinE SEra

428 Fetal bovine serum, Eu approved RM1112 10499 - - - Origin : Brazil Gamma irradiated

429 Fetal bovine serum, Eu approved RM9955 10500 - - - Heat inactivated

430 Fetal Bovine Serum, Eu approved RM10432 10270 - DE14-801 - Origin: Brazil Sterile filtered

431 Fetal bovine serum RM9952 10437 F0926 14-502 SH30910 USDA approved

432 Fetal bovine serum RM9951 10099 12003C DE14-701 SH30370 Origin : Australia

433 Fetal bovine serum RM9954 12483 F1051 - SH30397 Origin : Canada

434 Fetal Bovine Serum RM9970 - 12107C 14-471 SH30088.IR Origin : US Gamma irradiated

435 Fetal Bovine Serum RM10409 16140 F4135 14-503 SH30088.HI Origin: US Heat inactivated

436 Fetal Bovine Serum RM10434 26140 F6178 - SH30088 Origin: US Sterile filtered

Sera

*Country of origin might differ.Quality parameters might differ with each supplier.

42


Description


Sr.No.


SiGMaProduct Code

LOnzaProduct Code

HycLOnEProduct Code

SPEciaLity SEra

437 Fetal Bovine Serum, Eu approved RM10416 12676 F6765 DE14-820 SH30068 Charcoal Treated (US) (US)

438 Fetal Bovine Serum* RM10435 16141 / - DE14-850 SH30070 ES Cell tested, Store below (-200)C 30044 / (US) 10439

nEw BOrn caLF SEruM

439 new Born calf serum RM10437 26010 N4762 - SH30118.HI Heat inactivated (New Zealand) (US) Origin : Australia

440 new Born calf serum RM10422 16010 N4637 DE14-417 SH30118 Sterile filtered (New Zealand) (US) (US) Origin : Australia

441 new Born calf serum RM10421 - N4887 - SH30118.IR Gamma Irradiated (US) Origin : Australia

OtHEr aniMaL SEra

442 Horse serum RM1239 - - - SH30074.IR Donor herd (US) Gamma irradiated

443 Horse serum RM10436 16050 H1270 14-403 SH30074 Donor herd (New Zealand) (US) Sterile filtered

444 Horse serum RM10674 26050 H1138 - SH30074.HI Heat inactivated (New Zealand) (US)

445 Pig Serum RM10415 26250 P9783 - SH30908 (Germany) (New Zealand) (US Origin) (New Zealand)

446 rabbit serum RM10433 16120 R4505 - - (US)

Sera

*Country of origin might differ.Quality parameters might differ with each supplier.

43


Description


Sr.No.


SiGMaProduct Code

LOnzaProduct Code

HycLOnEProduct Code

SEruM-FrEE GrOwtH SuPPLEMEntS

447 itS Supplement 100x, Liquid TCL064 41400 I3146 17-838 SV30085 w/ 1 mg per ml Insulin from bovine pancreas, 0.55 mg per ml Human Transferrin and 0.5 μg per ml Sodium Selenite in Earle’s Balanced Salt Solution (500x) w/o Phenol red

448 itS-E Supplement 100x, Liquid TCL065 51500 S4920 17-839 - w/ 1 mg per ml Insulin from bovine pancreas, 0.55 mg per ml Human Transferrin, 0.5 μg per ml (500x) Sodium Selenite and 0.2 mg per ml Ethanolamine in Earle’s Balanced Salt Solution w/o Phenol red

449 itS-P Supplement 100x, Liquid TCL066 51300 - - - w/ 1 mg per ml Insulin from bovine pancreas, 0.55 mg per ml Human Transferrin, 0.5 μg per ml Sodium Selenite and 12 mg per ml Sodium pyruvate in Earle’s Balanced Salt Solution w/o Phenol red

450 itS-B Supplement 100x, Liquid TCL067 - - - - w/ 1 mg per ml Insulin from bovine pancreas, 0.55 mg per ml Human Transferrin, 0.5 μg per ml Sodium Selenite and 50 mg per ml Bovine serum albumin in Earle’s Balanced Salt Solution w/o Phenol red

451 Hat Medium supplement, 50x, liquid TCL072 21060 - - -

452 Ht Medium supplement, 50x, liquid TCL073 11067 (100x) - - SH30614

453 Lipid Supplement, 100x TCL091 11905 L0288 - SH30554

454 cholesterol Supplement, 1000x TCL092 - - - -

455 GSEM Supplement, 50x Liquid TCL088 - G9785 - -

Serum Free Growth Supplements

44


Description


Sr.No.


SiGMaProduct Code

LOnzaProduct Code

HycLOnEProduct Code

MiScELLanEOuS rEaGEntS

456 Bovine albumin fraction v 7.5% Solution TCL036 15260 A8412 - - In Dulbecco’s Phosphate Buffered Saline

457 Bovine albumin fraction v 30% Solution TCL037 - A9576 - - In Dulbecco’s Phosphate Buffered Saline

458 Erythrocin B 0.1% Solution TCL025 - - - - in Dulbecco’s Phosphate Buffered Saline

459 Gelatin Solution 2% TCL059 - G1393 - - w/ 2% Type B Gelatin from bovine skin in sterile tissue culture gradeater

460 d-(+)-Glucose Solution 10% TCL076 - G8644 - - w/ 10% D-(+)-Glucose in sterile tissue culture grade water, autoclaved

461 d-(+)-Glucose Solution 45% TCL077 - G8769 - - w/ 45% D-(+)-Glucose in sterile tissue culture grade water, autoclaved

462 L-Glutamine 200mM Solution TCL012 25030 G7513 17-605 SH30034 L-Glutamine in 0.85% normal saline Cell culture tested

463 HEPES 1M Solution TCL021 15630 H0887 17-737 SH30237

464 HiGlutaxLtM Supplement 200mM, 100x TCL030 35050 G8541 BE17-605UI SH30590 w/ L-Alanyl-L-Glutamine in 0.85% normal saline

465 1n Hydrochloric acid Solution TCL003 - H9892 - -

466 Glycerol Solution 50% TCL084 - - - -

467 Glycerol Solution 85% TCL085 - - - -

468 Phenol red 0.5% Solution in dulbecco’s Phosphate Buffered Saline TCL004 - P0290 - -

469 Pluronic F-68 Solution 10%, 100x TCL023 24040 P5556 - SH30594

470 Potassium bicarbonate Solution 7.5% TCL024 - P7682 - - (Powder)

471 recombinant Human insulin Solution, 10mg/ml TCL035 - I9278 - -

472 Sodium bicarbonate Solution 7.5% TCL013 25080 S8761 17-613 SH30033

473 1n Sodium Hydroxide TCL002 - S2770 - -

474 Sodium Pyruvate Solution 100mM TCL015 11360 S8636 13-115 SH30239

475 trypan blue 0.4% Solution in dulbecco’s Phosphate Buffered Saline TCL046 - T8154 - SV30084

476 trypan Blue 0.5% Solution in dulbecco’s Phosphate Buffered Saline TCL005 - - - -

Miscellaneous Reagents

45


Description


Sr.No.


SiGMaProduct Code

LOnzaProduct Code

HycLOnEProduct Code

477 trypsin inhibitor from Soyabean 1x, Liquid TCL068 17075-029 T6414 - - w/ 1 mg per ml of Trypsin Inhibitor in Dulbecco’s Phosphate Buffered Saline (Powder)

478 tryptose Phosphate Broth 50x TCL009 - - - -

479 water, Sterile, Molecular Biology Grade dEPc treated, nuclease and Protease free TCL016 4387937 - - SH30538

480 water, Sterile, Molecular Biology Grade Endotoxin tested dEPc treated, nuclease and Protease free TCL018 - - - -

481 water, Sterile, tissue culture Grade TCL010 - W3500 17-724 -

482 water, Sterile, tissue culture Grade Endotoxin tested TCL019 15230 - - -

483 yeast Extract Solution, 150 g/l TCL041 18180 - - -

484 yeast Extract Solution, ultrafiltered, 200 g/l TCL045 18200 Y4375 - -

cELL aSSay kitS

485 EzcounttM Mtt cell assay kit CCK003 V13154 CGD1 - -

486 Ez BluetM cell assay kit CCK004 DAL1 TOX8 - -

MycOPLaSMa dEtEctiOn kitS

487 EzdetecttM daPi Stain kit for Mycoplasma detection CCK007 - - - -

488 EzdetecttM Hoechst Stain kit for Mycoplasma detection CCK008 - MYC1 - -

Cell Culture Kits & Miscellaneous Reagents

Documents

Production · All equipments used for production as well as quality assurance are validated as per . prescribed guidelines. Equipment validation improves production processes and