1

Annex A

M. PHIL PROGRAMME IN MOLECULAR”BIOLOGY, VIROLOGY and IMMUNOLOGY

NATIONAL CENTRE OF EXCELLENCE IN VIROLOGY AND IMMUNOLOGY

For M. Phil Degree, 24 credit hours course work +06 credit hours for one year of original

research work and for Ph.D degree additional 18 credit hours course work + 30 credit hours of

original research work will be required.

M Phil. Semester-I Course No. Course Title Credit Hours Status

MVI-800 Gen & Mol. Virology 3 Core

MVI-810 Cell and Mol. Biology 3 Core

MVI-930 Biosafety and Bioethics 3 Core

MVI-830 Gen & Mol. Immunology 3 Core

Semester –I Total Credits 12

M.Phil. Semester-II Course No. Course Title Credit Hours Status

MVI-804 Bacteriophage Biology 3 Core

MVI-860 Viral Pathology 3 Core

MVI-850 Signal Transduction 3 Core

MVI-901 Bioinformatics 3 Elective

MVI-xxx Elective – II 3 Elective

Semester – II Total Credits 12

MVI-899 M Phil. Thesis Research 6 Compulsory

Total Credits 30

List of Elective_II. Courses MVI-810 Recombinant DNA techniques (3 Credit Hours) MVI-820 Microbiology (3 Credit Hours) MVI-834 Immune Pharmacology (3 Credit Hours) MVI-880 Molecular & General Toxicology (3 Credit Hours) MVI-920 Lab. Diagnostic Techniques (3 Credit Hours)

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Annex B

PH D COURSE WORK IN MOLECULAR”BIOLOGY, VIROLOGY and IMMUNOLOGY

NATIONAL CENTRE OF EXCELLENCE IN VIROLOGY AND IMMUNOLOGY PhD. Semester-I Course No. Course Title Credit Hours Status

MVI-810 Adv. in Mol. Biology 3 Core

MVI-801 Adv. in Virology 3 Core

MVI-xxx Elective – I 3 Elective

Scientific writing and presentation skills Non credit course

Total Credits 9

PhD. Semester-II Course No. Course Title Credit Hours Status

MVI-851 Medical Genetics 3 Core

MVI-870 Vaccinology 3 Core

MVI-xxx Elective – II 3 Elective

Total Credits 9

MVI-999 PhD Thesis Project: 30

Total Credits 48 (excluding MPhil Credits)

Elective Courses: MVI-840 Trends in Pharmacology MVI-852 Immunogenetics MVI-861 Molecular Mechanisms of Disease MVI-910 Stem Cell Studies MVI-940 Experimental Therapeutics MVI-950 Plant viruses and diseases

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Annex C

NCVI Core Curriculum MPhil/PhD program

Following is the list of course that will be initially offered in NCVI. With the induction of more students and faculty, additional course will be offered and some will be modified. The changes will be made periodically by the academic body of NCVI and sent to Academic and Examination (A & E) body of NUST for final approval.

Major Courses CODE List

Serial No Course Code Course Title .

1 MVI-80 Virology

2 MVI-81 Molecular and Cell Biology

3 MVI-82 Microbiology

4 MVI-83 Immunology/Immune disorders

5 MVI-84 Pharmacology (Viral, Immune etc)

6 MVI-85 Medical Genetics/ Targeted Gene Therapy

7 MVI-86 Viral Pathology

8 MVI-87 Vaccinology

9 MVI-88 Metabolism/Toxicology

10 MVI-89 Biotechnology/Fermentation

11 MVI-90 Bioinformatics

12 MVI-91 Stem Cell Studies

13 MVI-92 Lab Diagnostic Techniques/Instrumentation

14 MVI-93 Biosafety/Bioethics

15 MVI-94 Experimental Therapeutics

16 MVI-95 Plant viruses and diseases

Each course has further Subdivisions as listed below.

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Serial No

Course Code Course Title .

1

MVI-80

Virology

CODE CONTENTS 800 GENERAL VIROLOGY 801 Basic Concepts in Virology 802 ADVANCES IN VIROLOGY

803 MECHANISTIC VIROLOGY

804 Bacteriophage Biology 805

2

MVI-81

Molecular Biology/Cell Biology

CODE CONTENTS 810 MOLECULAR BIOLOGY

811 CELL BIOLOGY-I

812 CELL BIOLOGY -II

813 GENOMICS AND PROTEOMICS

814 815

3

MVI-82

Microbiology

CODE CONTENTS

820 MOLECULAR MICROBIOLOGY_1

821 MOLECULAR MICROBIOLOGY_2

822 823 824 825

4

MVI-83

Immunology/Immune Disorders

CODE CONTENTS 830 CLINICAL IMMUNOLOGY 1 831 CLINICAL IMMUNOLOGY 2 832 ANTIVIRAL IMMUNOLOGY

833

834 835

5

MVI-84

Pharmacology (Viral, Immune etc)

CODE CONTENTS 840 PHARAMACOLOGY

841 842 843 844 845

6

MVI-85

Medical Genetics/ Targeted

Gene Therapy

CODE CONTENTS 850 GENERAL GENETICS

851 MEDICAL GENETICS

852 IMMUNOGENETICS

5

853 GENETHERAPY

854 855

7

MVI-86

Microbial Pathology

CODE CONTENTS 860 Viral Pathology 861 Molecular Mechanisms of Disease 862 863 864 865

8

MVI-87

Vaccinology

CODE CONTENTS 870 VACCINOLIOGY

871 872 873 874 875

9

MVI-88

Metabolism/Toxicology

CODE CONTENTS 880 MOLECULAR TOXICOLOGY

881 882 883 884 885

10

MVI-89

Biotechnology/Fermentation

CODE CONTENTS 890 BIOTECHNOLOGY

891 892 893 894 895

11

MVI-90

Bioinformatics

CODE CONTENTS 900 BIOSTATISTICS

901 BIOINFORMATICS

902 903 904 905

12

MVI-91

Stem Cell Studies

CODE CONTENTS 910 STEM CELL STUDIES 911 912 913

6

914 915 916

13

MVI-92

Lab Diagnostic

Techniques/Instrumentation

CODE CONTENTS 920 LAB DIAGNOSTICS/INSTRUMENTATION

921 922 923 924 925

14

MVI-93

Biosafety/Bioethics

CODE CONTENTS 930 ISO-9007 931 932 933 934 935

15

MVI-94

Experimental Therapeutics

CODE CONTENTS 940 EXPERIMENTAL THERAPEUTICS

941 942 943 944 945

16

MVI-95

Plant Viruses and Diseases

CODE CONTENTS 950 PLANT VIROLOGY

951 STRATEGIES TO COMBACT PLANT VIRUSES

952 953 954 955

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MVI-800: GENERAL VIROLOGY (3 CREDIT HOURS)

COURSE CONTENTS: This is an introductory level course that describes the history of virology,

development of the concepts of viruses, and various biological and molecular aspects of bacterial,

animal and plant viruses. The course is divided into 3 parts.

COUSRE CONTENTS:

200 years of discoveries

General aspects of virology

Viral Classification and Structure

Modes of infection

Cellular Models of virus propagations

Replication and pathogenesis in a comparative fashion.

Host-virus interactions

Transformation and oncogenesis

Immunopathology

Host defense mechanisms

Antiviral pharmacology and applied virology

Bacteriophages

Plant viruses and their associated diseases

What is next for Virology?

MVI-800: BASIC CONCEPTS IN VIROLOGY (3 CREDIT HOURS)

LEARNIG OBJECTIVES: Viral life cycle, mode of replication and infection will be discussed.

Cytopathic effects of viral infection, lytic and lysogenic infection and viral gene expression and host

defenses will be discussed.

COURSE CONTENTS:

Introduction to Bacteriophages and viruses

Virus Genomes/ Viral Genetics: Linear, Circular, DNA or RNA

Viral Infection: Attachment, penetration, uncoating

Viral Gene Expression: Early and late gene expression

Viral Replication: Lytic and latent life cycles

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Viral Pathogenesis: Virulence, Cytopathic effects, Host responses

Virus spread through insect vectors

Phenotype mixing and pseuodtypes

Viral Transformation

Prions, Viroids and Virosoids

RNA transforming viruses

DNA transforming viruses

Host Defense Mechanisms

Apoptotic or Anti-apoptotic Viruses

Immune activation

Anti-Viral Pharmacology

Antiviral drugs

BOOKS RECOMMENDED:

Lytic mode of lambda development by D. Freidman and M. Gottesman, cold spring harbor

laboratory, cold spring harbor, N.Y.

Single stranded DNA by R.L. Sinsheimer.

Principles of Virology by S.J. Flint.

Fields Virology by B. Fields

Principles of Virology by Knipe.

Principles of Virology by Alan J. Cann.

MVI-802: ADVANCES IN VIROLOGY (3 CREDIT HOURS)

LEARNING OBJECTIVES: Details of the life cycle of different classes of viruses will be

thought. This course is deigned in such as way that students will be able to distinguish target specific

pathways of various classes of viruses. By understanding the common factors that contribute to

diseases caused by viral infections, it will help students to develop strategies to prevent or resolve

such infections.

COURSE CONTENTS

Hepresvirues (HSV-1, HSV-2, HCMV, EBV, PRV, VZV, HSV-6, HSV-8)

Retroviruses (HIV-1, HIV-2, HTLV-1, HTLV-2)

Defective Retroviruses and Endogenous Retrotransposons

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Hepatitis viruses (HAV, HBV, HCV, HDV, HEV, HGV)

Entroviruses (Coxsackieviruses, Echovirus, Hepatitis A, Norwalk and Norwalk like viruses,

Reovirus, and Rotavirus)

Influenza and cold viruses

Neurotropic viruses [lymphocytic choriomeningitis virus (LCMV) and Measles virus (MV)]

Adenoviruses (Ad-1, Ad-2, Ad-6)

Picornaviruses (Poliovirus, rhinovirus)

Togaviruses (Rubella, Yellow fever, encephalitis)

Polyoma viruses (SV40, JC, and Papilloma)

Coronaviruses

Parvoviruses (ssDNA)

Reoviruses (dsRNA)

Poxviruses

Plus stranded RNA viruses vs. Negative-stranded RNA viruses

Yeast viral like particles (VLPs) (Ty-1, Ty-2, Ty3)

Insect viruses (Dengue, Tick born)

Primate viruses (Ebola and Marburg)

Avian viruses (Bird flu)

BOOKS RECOMMENDED:

Hepatitis B and C carrier to cancer by Sarin.

Retroviruses by Coffin and John M.

Human Retroviruses by Bryan R.

Interaction by retroviruses and Herpes viruses by H. J. Kung.

Human Papilloma viruses by D.J.McCance.

Human Papilloma viruses by Clare (EDT) Davy.

The coronaviridae by Stuart G. Siddenn.

Molecular Biology of Hepatitis B virus by Koshy.

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MVI-803: MECHANISTIC VIROLOGY (3 CREDIT HOURS)

LEARNING OBJECTIVES: Details of the potential mechanism(s) of viral replication and

transcription and translation control will be thought. This course is deigned in such as way that

students will be able to design antiviral approaches that will target specific pathways of viral life

cycle.

COURSE CONTENTS

Oncogenic viruses and potential role of oncogenes

Neurotropic viruses and neurovirulence

Balance between neuroprotection and immunopathogenesis

Mechanism(s) of latency

Packaging/Encapsidation of viral genome

Mechanisms of replication of RNA viruses

Mechanisms of replication of DNA viruses

Control for viral RNA processing

Control of RNA transport and stability

Mechanism of antiviral therapy

Cell to cell spread of viruses

Glycobiology relevant to viral pathogenesis and control

Intracellular Transport mechanism(s) of mature viral particles

Signal transduction pathways perturbed by viral infection and/gene expression

Cellular and animal model of virus propagation

Use of viral vectors as gene therapy

Development of surrogate/pseudo type models for viral genes expression

LAB PRACTICALS

Bacteriophage Isolation (M13, lambda, Mu)

Transduction vs. transposition assay

Purification of replicative forms of M13 ssDNA

Viral isolation and Plaque Assay (Hepresviruses, HSV-1 and HCMV)

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MVI-804: BACTERIPHAGE BIOLOGY (3 CREDIT HOURS)

LEARNING OBJECTIVES: Historical overview of pages will be presented. The structural

organization, genetics, attachment, penetration and their replication will be discussed. The

bacteriophages of marine bacteria constitute the highest amount of biodiversity in nature which will

be compared with terrestrial bacteriophage ecology. The use of bacteriophages as therapy to cure

multi-drug resistant bacteria, for cleaning the contaminated polluted rives/garbage will be discussed

COURSE CONTENTS:

General properties of phages

Historical overview of phages

Classification of gram positive and gram negative phages

Large DNA, small DNA and RNA phages

Attachment and Penetration

Synthesis of protein and nucleic acids

Bacteriophage lysis: Mechanism and regulation

Phages and transposable elements (Mu Biology)

Lambda phage

T3, T7 and T4 phages

P1 (Cre lox) system

Defective phages and phage-like objects

Evolution and natural biology of phages

Evolutionary conservation and relation to eukaryotic viruses

Relation of bacteriophages to bacterial ecology

The health value of bacteriophages (Phage Therapy)

Synthetic bacteriophages

Use of bacteriophages in Physics (Nano wires and electrodes)

Biocide bacteriophages for environmental surfaces

Bacteriophages of industrial importance

Control of bacteriophage contamination

12

MVI-810: MOLECULAR BIOLOGY (3 CREDIT HOURS)

LEARNING OBJECTIVES: This course will discuss basic principles of molecular biology,

structures of macromolecules with emphasis on the mechanisms that dictate the fundamental

processes for the transfer of DNA->RNA-> protein.

COURSE CONTENTS:

Structure and origin of RNA and DNA

Molecular structure and organization of Genes, Genome and Chromosomes

Gene Transcription

Regulation of Transcription Initiation, role of GTF and TSFs

DNA Replication

DNA Recombination (site specific and non-homologous)

RNA Processing, Nuclear Transport, and Post-Transcriptional Control

Control of Translation

Selection of mRNAs to be translated

mRNA degradation and interference

Protein activity: Post-translational modifications, activation, localization, and degradation

Protein structure and function

LAB PRACTICALS

Nucleic Acid Biochemistry and Analysis:

DNA isolation (Plasmid and Chromosomal)

Agarose and Polyacrylamide Gel Electrophoresis

Electrophoresis Mobility Shift Assays (EMSA)

Chromatography

Spectrophotometer Techniques

Molecular Cloning and subcloning

Vector preparation, restriction mapping

Polymerase chain reaction (PCR) of a target gene

Agarose gel electrophoresis of gene PCR product

13

Analysis of restriction digest, ligation into a vector

Transformation to E. coli and yeast

Selection and Screening, Plasmid preparation and large scale purification

Southern Blot vs. Northern blot analysis

DNA sequencing and Alignments-Review bioinformatics tools

Protein Translation: Open Reading Frames

BOOKS RECOMMENDED

Molecular Biology of the Gene by Watson, J. D., T. A. Baker, S. P. Bell, A. Gann, M. Levine, and

R. Losick, Eds., (2003). (5th edition) New York, Benjamin Cummings ISBN 0-8053-4635-X

The Cell by Bruce Albert and Dennis Bray, (3rd Edition), Garland Publishing Inc, New York and

London Gene VIII By Lewin Benjamin Eds 2004. Oxford University press, Inc, New york.

Biochemistry by Victor L. Davidson, Donald B. Sittman. 3rd Edition, 1993, Harwal Publication Co.

Cell and Molecular Biology by Gerald Karp.1996 John Willey and Sons, Inc. London.

Genetics by Peter J. Russel. 5th Ed. 1997, Benjamin-Cummings Publishing Company.

MVI-811: CELL BIOLOGY-I (3 CREDIT HOURS)

LEARNING OBJECTIVES: This course is designed to help students understand workings of the cell structure and function at

cellular and molecular level. In this course molecular basis of cell structure and function is

discussed which will provide students a detailed account of functional physiology of the cells.

COURSE CONTENTS:

Introduction

Cell Organization

Cell Architecture

Membrane Structure and Function

Bio Transport

Vesicular Transport

Transport Signals

Nuclear Transport

Bio Energetic

Mitochondrial Energy Conversion

14

Chloroplast Energy Conversion

Cytoskeleton

Cell Shape

Cell Contractility

Cell to cell Communication

Electrochemical Signaling

Synaptic and Sensory Transduction

Biochemical Signaling

Receptor Ligand Interactions

Second Messengers

Signaling Cascades

Cell Cycle and Apoptosis

Phases of Cell Cycle and Cell Division

Regulation of Cell Growth and Death

Specialized Cell Systems

BOOKS RECOMMENDED:

Gene VIII By Lewin Benjamin, Eds 2004. Oxford University press, Inc, New york.

Molecular Biology of the Gene by Watson, J. D., T. A. Baker, S. P. Bell, A. Gann, M. Levine, and

R. Losick, 5th Ed. 2003. New York, Benjamin Cummings ISBN 0-8053-4635-X

Molecular Biology of the Cell by Bruce Albert and Dennis Bray, 3rd Ed. Garland Publishing Inc,

New York and London.

Biochemistry by Victor L. Davidson, Donald B. Sittman. 3rd Ed. 1993, Harwal Pub Co.

Cell and Molecular Biology by Gerald Karp.1996 John Willey and Sons,Inc. London.

MVI-812: MEMBRANE TOPOLOGY & SIGNAL TRANSDUCTION (3CREDIT HOURS)

LEARNING OBJECTIVES:

This course is designed to help students understand workings of the cell structure and function at

cellular and molecular level that various viruses utilize in their life cycles. In this course molecular

basis of cell structure and function is discussed which will provide students a detailed account of

15

functional signaling cascade physiology of the cells. Furthermore, this course will discuss the key

components of the immune system at cellular and molecular level.

COURSE CONTENTS:

Cell Adhesion and ECM

Cell adhesion molecules and protein targeting

Collagen Matrix and Non-collagen Matrix

Metastasis

Protein sorting

An overview of protein targeting

Peroxisomal protein targeting

Secretary protein targeting

Membrane proteins

Functions of cell surface receptors

Post-translation modifications

Golgi protein sorting

Vesicular Transport

Cytoskeleton and force generation

Actin dynamics and Actin myosin interaction and regulation

Microtubule dynamics (dynamic instability model)

MAPS and microtubule motors

Cilia and flagella structure and movement

Pathways of intracellular signal transduction

P38 MAPK, JNK, ERK pathways

RAS and RAF pathways

Oxidative stress and anti-stress signaling (GST, MnSOD)

GTPase and their role in cancer

Ion channels expression, regulation and its relations to diseases

Purinergic signaling (p2X and p2Y)

PKA, PKB (AKT) PKC pathways

16

JAK/STAT and TGF-ȕ/smad pathways

NF-せB signaling

Hedgehog Wnt and Notch pathways

G-Protein coupled membrane receptors signaling

Steroid hormones and nuclear receptors signaling

Neurotransmitters, Opioids receptors and Eicosanoids signaling

Peptide hormones and growth factors signaling

Feedback and crosstalk signaling network

Cell Signaling and its Applications in Disease and Therapeutics

CELL BIOLOGY LAB PRACTICALS (01 CREDIT HOUR)

Cell and Tissue Culture

Membrane fractionation

Transient and Stable DNA Transfections

Tissue processing

Immunohistochemistry

Microscopy

Chromatin Immunoprecipitation

BOOKS RECOMMENDED:

Gene VIII By Lewin Benjamin Eds 2004. Oxford University press, Inc, New york.

Molecular Biology of the Gene by Watson, J. D., T. A. Baker, S. P. Bell, A. Gann, M. Levine, and

R. Losick, 5th Ed. 2003. New York, Benjamin Cummings ISBN 0-8053-4635-X

The Cell by Bruce Albert and Dennis Bray, 3rd Ed. Garland Publishing Inc, New York and London.

Biochemistry by Victor L. Davidson, Donald B. Sittman. 3rd Ed. 1993, Harwal Pub Co.

Cell and Molecular Biology by Gerald Karp. 1996, John Willey and Sons,Inc. London.

NCVI-813: GENOMICS AND PROTEOMICS (3 CREDIT HOURS)

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LEARNING OBJECTIVES: This course aims at offering an insight into genomics and proteomics

of biological systems, their diversity and various aspects as well.

COURSE CONTENTS:

Yeast genome as a model eukaryotic genome system

Genome diversity and evolutionary time-scale

Human Genome project, it implications on viral infections

Quantitative genetics

Linkage, crossing over and gene mapping in Prokaryotes

Linkage, crossing over gene mapping in Eukaryotes

Introduction to DNA microarrays

Expression profiling using microarrays

DNA microarray experimental design

DNA microarray data analysis and interpretation

Application of functional genomic research in the study of viruses

Proteome analysis

Techniques of proteomics

Proteomes in health and viral disease

Limitations and future development

BOOKS RECOMMENDED

Genomics, Proteomics and Bioinformatics by A. Malcolm Campbell and Laurie J. Heyer. 2002.

Benjamin Cumming Publishing Company.

Proteomics: From protein sequence to function by Pannington, S and M.J.Dunn. 2001. Spring

Verlag.

Introduction to Proteomics, Tools for the New Biology by Daniel C. Liebler. 2001. Humana

Press.

DNA Microarray: A molecular cloning manual by David Bowtell and Joseph Sambrook. 2003.

Cold Spring Harbor Laboratory Press, New York.

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MVI-820: MOLECULAR MICROBIOLOGY (3 CREDIT HOUR)

LEARNING OBJECTIVES: This is an introductory level course that describes various

biological and molecular aspects of bacteria. Our coverage will focus almost entirely on

bacteria that infect humans and cause serious disease.

COURSE CONTENTS:

The Bacterial Cell: An introduction to the structure of the bacterial cell.

Bacterial identification in the diagnostic laboratory versus taxonomy.

Taxonomic characterization of bacteria. Approaches to rapid diagnosis

Nutrition, Growth and Energy Metabolism: Anaerobic and aerobic metabolism. Metabolism

of sugars and fatty acids

Cell Envelope, spores and Macromolecular Biosynthesis: Structure and synthesis of the cell

walls of gram-positive and gram negative bacteria

Antibiotics - Cell Envelope: The mode of action of beta-lactate antibiotics.

Antibiotics - Protein Synthesis, Nucleic Acid Synthesis and Metabolism: The mode of action

of antibacterial chemotherapeutic agents. Antibiotic susceptibility testing.

Genetic Exchange: The mechanisms of gene transfer in bacteria; Insertion sequences,

transposable genetic elements and plasmids.

Genetic Regulatory Mechanisms: The structure and transcription of bacterial genes. The

molecular mechanisms that bacteria use to regulate gene activity. Inducible and repressible

operons. The molecular mechanisms involved in catabolite repression and attenuation. The

ways bacteria regulate enzyme activity.

Enterobacteriaceae: Enterobacteriaceae, Vibrio, Campylobacter and Helicobacter

Streptococci: Groups A, B and D streptococcus, pathogenesis, diagnosis. Streptococcus

pneumonia and Staphylococci. Streptococcus and pneumonia, Staphylococcus infections,

food poisoning, toxic shock Neisseria and Spirochetes: Syphilis, Lyme disease,

leptospirosis, gonorrhea, meningitis.

Anaerobes and Pseudomonas - Opportunistic Infections. Clostridia, gas-gangrene, tetanus,

botulism, pseudomonads.

Zoonoses: Listeria, Francisella, Brucella, Bacillus and Yersinia Plague, Anthrax,

Brucellosis, Listeriosis.

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General Aspects of Bacterial Pathogenesis: Exotoxins and endotoxins

LAB PRACTICALS

General Microbiological Laboratory Techniques

Media preparation

Identification of gram negative and positive strains

Growth curves for bacteria

Bacteriophage isolation

Bacterial conjugation for Plasmids transfer

Genetic complementation

Biosafety Guidelines

BOOKS RECOMMENDED

Medical Microbiology & Immunology by Warren Levinson & Ernest Jawetz. 7th Ed. 2003.

McGraw-Hill Publications. ISBN 0-07-122973-6.

Principles of Virology, Molecular Biology, Pathogenesis, and Control by S.J. Flint, L.W.

Enquist, R.M. Krug, V.R. Racaniello, and A.M. Skalka. 2nd Ed. 2000. ASM Press.

Fundamental Virology edited by D.M. Knipe and P.M Howley. 4th Ed. 2001, Lippincott Williams

and Wilkins.

MVI-830: BASIC IMMUNOLOGY (3 CREDIT HOURS)

LEARNING OBJECTIVES: This course will cover basic concepts of immunology and the

mechanism by which T and B lymphocytes acquire high level of specificity. Surface molecules will

be described in detail, followed by accessory and natural killer cells and human lymphocyte antigen.

Autoimmunity and tolerance will also be discussed.

COURSE CONTENTS:

The immune system

Origins of the immune system

Antibody structure and function and antibody diversity

Helper T cells and their activation

Organs of the lymphatic system

20

T lymphocyte development and differentiation

B cell lymphocyte Development and Differentiation

Cell-cell interaction (T cells and Antigen presentation cells)

Non specific Defense Cells (Natural Killer cells)

Monocytes and Dendritic cells

HLA system (genomic organization, molecule structure, class I/II alleles and antigen

presentation).

The complement system

Innate immunity

Leukocyte migration

Pathological Immune mechanisms and tolerance

BOOKS RECOMMENDED

Introduction to Immunolgy by J.W. Kinhall, 1983, Macmillan Pub Co.

Immunology by Richard A.Goldsby, Thomas j.Kindt, Barbara A. Osborne, Janis Kuby, 2005. W.H

Freeman and Co.

Cellular and Molecular Immunology by Abbas, 2005. Elsevier Pub Co.

Color Atlas of Immunology by G.R. Burmester, A. Pezzutto, 2006. Thieme Stuttgart, New York.

MVI-831: LABORATORY APPLICATIONS OF IMMUNOLOGY ( 3 CREDIT HOURS)

COURSE OBJECTIVE: This course is designed to describe techniques for antibody production,

antigen detection, Immunofluorescence, and Immunohistochemistry.

COURSE CONTENTS

Antibody production (monoclonal and polyclonal)

Antigen-Antibody interaction

Definitions and precipitation techniques

Techniques of electrophoresis

Agglutination Techniques/complement binding Reaction

ELISA, RIA, and Immunoblotting

Immunrofluorescence, Immunohistology

Cellular Immunity

21

Cell Isolation techniques

Test of T cell Functions

Antigen-specific Test

Assay procedure for characterizing antigen specific T cells

Humoral Immunity

Test for B-cell Function

Molecular Biological Methods

Analytical Techniques

MVI-832: IMMUNE DISORERS. (3 CREDIT HOURS)

LEARNING OBJECTIVES: Major human diseases attributed due to defects in immune system

will be discussed and immunological mechanism that governs the onset of key human diseases will

be eluded for their potential as therapeutic potential. Genes that contributes for these defects will

also be studied.

COURSE CONTENTS

Immunodeficiencies (Sever combined immunodeficiency syndrome)

Humoral and cellular Immunodeficiencies

Granulocytic Deficiencies

Complement Deficiencies and Defects

HIV Structure and Replication

Course of HIV Infection

Diagnosis and Treatment of HIV Infection

Hemolytic diseases and cytopenias

ABO blood group system Rhesus and other blood group systems

Mechanisms of hemolytic and antigen detection autoimmune diseases

Drug induced hemolysis

Hemolytic due to warm antibodies

Hematological Diseases

Acute Leukemias

Overview of Lymphoma Classification

22

Hodgkin’s disease

T-Cell Lymphomas

B-Cell Lymphomas

Plasma Cell Dyscrasias

Multiple Myeloma

Cryoglulinemia

Amyloidsis

Tumor Immunology

Detection and identification of Tumor Antigens

Immune Escape Mechanisms of Tumor Antigens

Immunotherapeutic Strategies (I)

Immunotherapeutic Strategies (II)

Transplantation of Autologous

Bone Marrow/Heatopoietic Stem Cells

Transplantation of Allogenic bone Marrow/Hematopietic Stem Cells

Clinical Aspects of Organ Transplantation

MVI-833: CLINICAL IMMUNOLOGY 2 (3 CREDIT HOURS)

COURSE OBJECTIVE: Major human diseases attributed due to defects in immune system will be

discussed and immunological mechanism(s) that governs the onset of key human diseases will be

elucidated for their therapeutic potential.

COURSE CONTENTS

Musculoskeletal Diseases

Clinical features of Rheumatoid Arthritis

Synovial Changes in Rheumatoid Arthritis

Pathogenesis of Rheumatoid Arthritis

Juvenile Chronic Arthritis

Clinical features of Spondylarthritis

Pathogenesis of Spondylarthritis

Gout, Ploychondritis, and Bechet’s syndrome

23

Skin Diseases

Urticaria

Contact Allergies

Atopic Dermatitis and Leukocytoclastic vasculities

Psoriasis and Bullous Skin Diseases

Gastrointestinal Diseases

Atrophic Gastritis, Whipple’s disease and Sprue

Chronic Inflammatory Bowl Diseases

Autoimmune Liver Diseases

Respiratory Diseases

Bronchial Asthma and Allergic Rhinitis Sarcoidosis and Idiopathic

Pulmonary Fibrosis

Extrinsic Allergic alveolitis Tuberculosis

Renal Diseases

Immunological mechanisms

Glomerulonephritis (I)

Glomerulonephritis (II) and Interstitial Nephritis

Metabolic Diseases

Autoimmune Thyroid Diseases

Diabetic Mellitus and Autoimmune Polyglandular Syndrome

Heart disease

Rheumatic fever, Myocarditis, and Postinfection syndrome

Neurolgical Diseases

Multiple Sclerosis

Autoantibody-mediated Diseases

Myasthenia Gravis and Lambert-Eaton syndrome

Ophthalmic Diseases

Anatomy and Pathogenesis

Extraocular inflammations

Uveitis (I)

24

Uveitis (II) and Ocular Manifestation of Systemic Disease

Reproduction Immunology

Reproduction immunology

Gene therapy

Modern approaches and tools for gene therapy

JOURNALS RECOMMEDED

ANNUAL REVIEW OF IMMUNOLOGY, JOURNAL OF IMMUNOLOGY, EUROPEAN

JOURNAL OF IMMUNOLOGY, IMMUNOLOGY TODAY, ADVANCES IN IMMUNOLOGY,

CRC CRITICAL REVIEWS IN IMMUNOLOGY and HUMAN GENE THERAPY

MVI-840: PHARMOCOLOGY (Viral, Immune) (3 CRDIT HOURS)

Immune Pharmacology

(NSAIDS) and Glucocorticoids

Antimetabolites, Cyclophosphamide, Sulfasalazine, and Gold

Cyclosporin A, Mycophenolate, and Leflunomide

Nucleoside and non-nucleoside analogs

ATP-signaling and Viral response

Antiretroviral update

Xenobiotic Transport defects

Side effect of drugs and liver metabolism

Troxacitabine in acute leukemia

Varicella vaccine and fatal outcome in leukaemia

MVI-850: GENERAL GENETICS (3 CREDIT HOURS)

LEARNING OBJECTIVES: Medical genetics involves any application of genetics to medical

practice. The objectives of this course is to provide students with breadth and depth of knowledge to

make them realize the role of genetics in medicine and why the knowledge of medical genetics is

important for today’s health care facilitators and also for scientists and clinical researchers who are

interested in conducting research to elucidate the genetic basis of inherited disorders whose numbers

is increasing rapidly.

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COURSE DESCRIPTION:

This course includes detailed presentation of structure and function of genes, DNA analysis,

chromosomes and chromosomal aberrations; gametogenesis, Mendalian versus Non-Mendelian

inheritance; genomics and population genetics.

COURSE CONTENTS:

Medical Genetics in Perspective:

Developmental genetics-basic concepts

The human chromosomes

The life cycle of somatic cell

Meiosis

Human gametogenesis and fertilization

Medical relevance of mitosis and meiosis

The Human Genome: Structure and Function of Genes and Chromosomes:

DNA structure; A brief view

The central dogma: DNA RNA Protein

Fundamentals of gene expression

Structure of human chromosome

Variation in gene expression and its relevance in medicine

Techniques of Gene Analysis:

Analysis of individual DNA and RNA sequences

Methods of nucleic acid analysis

In Situ Hybridization to chromosomes

DNA sequence analysis

Methods of protein analysis

Pattern of Single-Gene Inheritance:

Terminology

Genetic disorders with Classical Mendelian Inheritance

Autosomal recessive inheritance

Pattern of autosomal dominant inheritance

X-linked inheritance

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Atypical pattern of inheritance

Genetic Variation in Populations:

Genetic diversity in human populations

Phenotypes, genotypes and gene frequencies

The Hardy-Weinberg law

Frequency of X-linked genes and genotypes

Factors that disturb Hardy-Weinberg equilibrium

DNA profiling- a practice test of Hardy-Weinberg assumptions

Gene Mapping and Human Genome Project:

Physical mapping of human genes

Mapping human genes by linkage analysis

Applications of human genome mapping

The human genome project

RECOMMENDED BOOKS:

Essential of Genetics by William Klug, Michael Cummings. Pearson Pretice Hall 2005.

Human Genetics by Ricki Lewis. McGraw Hill 2005

Genetics in Medicine: Robert Nussbauon, Roderide Mclnnes by Huntington Willard Thompson and

Thompson. Saunder 2004

Medical Genetics by Lynn Jorde, John, Michael Bamshad, Raymond White. Mobsy 2003.

Medical Genetics at a Glance by Dorian Pritchard, Bruce Korf. Blackwell S. Publications 2003.

MVI-851: MEDICAL GENETICS (3 CREDIT HOURS)

LEARNING OBJECTIVES: Emphasis is placed on the clinical application of Genetics. Such

topics include genetic assessment and counseling with prenatal diagnosis; chromosomal disorders;

single gene defects attributed due to viral integration; immunogenesis and cancer genetics;

multifactorial disorders congenital malfunctions; population screening; prevention and control;

treatment of genetic autoimmune immune diseases.

Clinical Cytogenetic:

Introduction to Cytogenetic

Chromosomal abnormalities

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Mendelian disorders with Cytogenetic effects

Cytogenetic analysis in cancer

Disorders of autosomes and sex chromosomes

Principles of Molecular Diseases:

Effect of mutation on protein function

Mutations disrupting the functions of biologically normal proteins

Hemoglobinopathies and HIV/HCV infection correlations

Molecular and Biochemical Basis of Genetics:

Diseases due to mutations in different classes of proteins

Enzyme Defects critical for viral infection

Defects in viral receptors proteins (chemokine receptors)

Disorders of structural proteins

Neurodegenerative disorders

Pharmacogenetic diseases

Genetics of Disorders with Complex Inheritance:

Genetic analysis of qualitative disease traits

Genetic analysis of quantitative traits

Genetic mapping of complex traits

Diseases with complex inheritance

Cancer Genetics:

Cancer as a genetic disorder

Characteristics of cancer cells

Genes that cause cancer-oncogenes; tumor suppressor p53, WLM, Rb, BRCC1

Genetic changes that cause some cancer-case studies

Cancer prevention, diagnosis and treatment

Diagnosis Strategies for Genetic Disorders:

Indication for prenatal diagnosis

Genetic counseling for prenatal diagnosis

Methods of prenatal diagnosis

Emerging technologies for prenatal diagnosis

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Laboratory investigations

Effect of prenatal diagnosis on the prevention and management of genetic diseases

Population screening (neonatal screening)

Overall impact of genetic disease

Types of genetic diseases

Current state of treatment of genetic diseases

Special consideration in treating genetic diseases

Treatment strategies

Genetic Counseling and Risk Assessment:

Genetic counseling

Case management in genetic counseling

Determining recurrence risks

Genetics and Society:

Population screening for genetic diseases

Ethical issues in medical genetics

Eugenic and dysgenic effects on gene techniques

RECOMMENDED BOOKS:

Genetics in Medicine by Robert Nussbauon, Roderide Mclnnes. Huntington Willard Thompson and

Thompson. Saunder 2004.

Essential of Genetics by William Klug, Michael Cummings. Pearson Pretice Hall 2005.

Human Genetics by Ricki Lewis. McGraw Hill 2005.

Medical Genetics by Lynn Jorde, John, Michael Bamshad, Raymond White. Mobsy 2003.

Medical Genetics at a Glance by Dorian Pritchard, Bruce Korf. Blackwell S.

MVI-852 IMMUNOGENETICS (3 CREDIT HOURS) INTRODUCTION

Genetics of AB and TCR genes

Mechanism of AB diversity

Somatic recombination

Somatic hypermutation

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B-cell receptor genes

T-cell receptor genes

RAG genes, Knockout mice

Inherited immune disorders.

Hyper Sensitivity

Autoimmunity

Immune Suppression.

MVI-860: VIRAL PATHOLOGY (3 CREDIT HOURS)

LEARNING OBJECTIVES: Major objective of this course will be to understand the viral

induced pathogenesis. The symptomatic of the disease and its progression into fatalities will be

discussed. The molecular pathways that lead to the induction of viral induced oral hairy leukoplakia,

insulin resistance, encephalitis, demyelination hemophagocytic Lymphohistiocytosis, choleostasis

hemorrhagic fever, hepatic fibrosis, cirrhosis and hepatocellular Carcinoma (HCC) will be studied.

COURSE CONTENTS

HCV -induced hepatic fibrosis

Viral induced fatty liver diseases (steatosis)

Hepatocellular carcinoma (HCC)

Viral induced arthrosclerosis

Viral induced ascitis

Viral induced insulin resistance

Viral meningitis and encephalitis

CMV retinitis

Falvi and foliovirus induced hemorrhagic fever

Antibody dependent enhancement (ADE) of Dengue Virus

Oncogenic viral pathology

Viral induced choleostasis

HIV/EBV induced Oral Hairy Leukoplakia (OHL)

Kaposi’s Sarcoma

Viral induced karatitis and conjunctivitis

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Viral induced myocarditis and inflammatory cardiomyopathy

Viral induced Emphysema

Virus induced autoimmune diseases

Virus Induced Demyelination

EBV-induced Hemophagocytic Lymphohistiocytosis

MVI-870: VACCINOLIOGY (3 CREDIT HOURS)

LEARNING OBJECTIVES: This course is deigned in such as way that students will be able to

design vaccines and their testing in yeast and mammalian cell, eggs and various animal models. The

history of vaccine, their efficacy, potential draw back/risks, impurities, cost effective, methods will

be discussed.

COURSE CONTENTS

History of vaccines

Viral proteins as potential targets for vaccine

Live versus attenuated vaccines

Role of multinational companies in vaccines production

DNA as vaccine

Peptide & Subunits vaccine

Adjuvants in vaccines

Population Genetic analysis: immunity to vaccine

Recombinant vaccine

Animal models of vaccine testing

Cost-effective approaches for production of new vaccines

Human testing and efficacy of vaccines: ethical issues

Recommendations of the Advisory Committee on Immunization Practices (ACIP)

Quadrivalent vaccine against human papillomavirus to prevent high-grade cervical lesions

Understanding the demand and supply of popular vaccine

Launching of vaccine and clinical trials

Anti-rabies immunoglobulin preparation based on F(ab')2 fragments

Effect of Panavir/Zanamivir on influenza A virus reproduction

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Vaccine safety

RECOMMENDED BOOKS:

Recombinant vectors in vaccine development by Fred Brown.

The Vaccine book by Barry R. Bloom.

Vaccine S by Plotkin_Orenstein.

Mass vaccine global aspect-progress and obstacles by Plotkin.

Pre-clinical and Clinical development of new vaccines by Fred Brown.

JOURNALS RECOMMENDED: VACCINE, JOURNAL OF ANTIMICROBIAL

CHEMOTHERAPY, CLINICAL IMMUNOLOGY AND IMMUNOPATHOLOGY, MEDICAL

MICROBIOLOGY AND IMMUNOLOGY

MVI-880: MOLECULAR TOXICOLOGY (3 CREDIT HOURS)

LEARNING OBJECTIVES: This course will provide a general understanding of the science of

toxicology as it relates to mankind and the environment at macro and molecular levels.

COUSE CONTENTS:

Fundamental concepts of Toxicology

Dose-response relationships

Effect of and viral infection and vaccines on liver toxicity

Absorption of toxicants, distribution and storage of toxicants

Biotransformation and elimination of toxicants.

Classes of toxins and their respective toxic effects on humans, animals and the environment.

The mechanisms of toxicity, describes medical, biochemical and genetic aspects of toxicology

including regulation of gene expression

Regulatory Toxicology, i.e., risk assessment and the chemo dynamics of viral contaminants in

the environment.

COURSE CONTENTS:

Part I: Basic Concepts

History and Basic Principles of Toxicology

Measurement of Toxicity: Toxicokinetics during viral infection

Absorption, Distribution, Storage, Elimination

Biotransformation: Metabolism and bioactivation of toxins

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Part II. Various classes of Toxicants:

Chemical Toxins

Toxicology of gaseous pollutants

Hydrocarbons

Halogenated hydrocarbons

Aromatic amines and N-nitroso compounds

Metals

Polymers

Cosmatics

Biochemical toxins

Insecticides and pesticides

Animal venoms and poisons

Plant toxins

Microbial toxins

Food and water borne toxins

Drugs and biopharmaceuticals

Part III. Mechanisms of Toxicity:

Organ System Toxicology

Hematotoxicity

Hepatotoxicity

Pulmonotoxicology

Nephrotoxicology

Neurotoxicology

Dermatotoxicology

Oto-oculartoxicology

Immunotoxicology

Genotoxicology

Carcinogenesis: DNA damage, DNA repair

Toxicogenomics: Induction and regulation of gene expression by toxins

Regulatory Toxicology:

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Toxicity assays

Risk Assessment

Controlling health hazards

Detoxification during and after viral infections

Bioremediation

Phytoremediation

BOOKS RECOMMENDED

Principles of toxicology by Williams and Roberts. 2nd Ed. A wiley Interscience publication.John

wiley and sons, Inc, New york

Toxicology by Hans Marquardt, Siegfried Schafer, Roger O. McClellan 1999. Academic Press Inc.

London.

Industrial Toxicology by Williams and Burson. 1989. John Willey and Sons.Inc.

Environmental Toxicology by Sethi, Iqbal, Satake, Mido. 2nd Ed.

Casarette & Doull’s Essentials of Toxicology by Curtis D.Klassen. John B. Watkins. 2003. Mc

Graw Hill.

Molecular Toxicology by P. David Josephy. Oxford University press.

Handbook of Toxicology by Michael Derelanko and Manfred Hollinger CRC Press 2004.

MVI-890: BIOTECHNOLOGY (3 CREDIT HOURS)

LEARNING OBJECTIVES: This course addresses the isolation, genetic manipulation, use of

organisms (commonly genetically modified) or their fermented food production, agriculture,

pharmaceutical discovery and production, molecular diagnosis, vaccine production, transgenic

animal formation, human gene therapy, forensic applications, microbial-base bioprocessing

pharming, bioterrorism and future of biotechnology. The course will consist of three parts:

COURSE CONTENTS:

Approaches of biotechnology

Specific applications

Social issues

I. APPROACHES OF BIOTECHNOLOGY:

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Structural Genomics:

Genome sequencing

Protein structure determination

Functional Genomics:

Bioinformatics

DNA microarray

Proteomics

ii) Modifying Protein Production and Function:

Protein engineering

Antisense technology

II. SPECIFIC APPLICATIONS:

Food Biotechnology:

Viral infection in food

Animals and microbes

Human Health and Diagnosis:

Viral diagnostics.

Forensic applications

Industrial Applications:

Bioprocessing

Microbial-based bioprocessing and Farming

Biosensors for detection of viruses

Recovering viral genomes and sequences

Environmental Applications of virus infection:

Bioremediation

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Waste management with virus contamination

Phytoremediation

Biofertilizers

SOCIAL ISSUES:

Rights and Privileges:

Intellectual property

Ethical implications

Risk and regulations

Perception and Fears about Viruses and Biotechnology:

Contrasting public and scientific perceptions of vaccines

Bioterrorism and bio defense

Biotechnology: The Future:

Purpose and expected outcomes

Technological advances in the pipeline

Third world participation in vaccine and biotechnology-bridging the gap

RECOMMENDED BOOKS:

Understanding Biotechnology: George Acquaah, Pearson Prentice Hall (2004).

MVI-900: BIOSTATISTICS (3 CREDIT HOURS)

LEARNING OBJECTIVES: This course is designed for students to understand different

computational biological algorithms, construct data models, use of molecular databases, use of

commonly available software for the analysis of bimolecular sequences and structures in viral and

cellular genes. They should be able to interpret the results, describe common post-genomic

experimental technologies associated with high-throughput data production.

COURSE CONTENTS:

Descriptive Biostatistics

Types of numerical data

Data sampling methods

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Graphical display of Data

Measurement of central tendency

Measurement of dispersion

Probability and probability distributions

Chi-Square Test

T-Test

Module II: Inferential Biostatistics

Sampling Distributions

Confidence Intervals

Baye’s Theorem

Hypothesis Testing

Experimental Design

Introduction to operating systems

Basics of Linux operating system

Words Processing, Spreadsheet and Presentation Skills

Internet for Biologists

Introduction to programming languages

Introduction to perl programming language

Programming skills in perl

Perl applications in bioinformatics

Introduction to databases

COBRA – Common Object Request Broker Architecture

MySQL database management system

MVI-901: BIOINFORMATICS ( 3 CREDIT HOURS)

Module I: Introduction to Bioinformatics

Definitions of bioinformatics and related fields

Objective and scope of bioinformatics

Kind of data used in bioinformatics

Data integration and data analysis

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Major biological databases

Module II: Sequence Alignment

Pairwise Sequence Alignment

Methods and tools of sequence alignment

Multiple Sequence Alignment

Module III: Phylogenetic Analysis

Phylogenetic trees

Methods of Phylogenetic analysis

Methods of evaluating phylogenies

Module IV: Gene Prediction

Introduction

Methods of gene prediction

Gene prediction tools

Module V: Protein Folding/Structure

Protein structure and its classification

Protein classification methodology and tools

Protein structure databases and visualization tools

Protein structure prediction

Protein function prediction

Module VI: Genomics and Proteomics

Introduction to genomics

Tools for genome analysis

Approaches for Genome-wide scan

Introduction to proteomics

Tools for proteome analysis

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LAB PRACTICLES:

Familiarization with Linux OS

Introduction to perl scripting

Creating a simple bioinformatics database

Accessing the publicly available databases

Pair wise and multiple sequence alignment

BLAST database and genome annotation

An Introduction to the vector/primer design Program

Protein structure prediction for known folds

Protein structure prediction for unknown folds

Visualization of biomolecular structures

BOOKS RECOMMENDED:

Fundamentals of Biostatistics by Bernard Rosner, Duxbury 2000.

An Introduction to Biostatistics by Glover & Mitchell, McGrawHill 2002.

Beginning of Perl for Bioinformatics by J Tisdall, O’Reilly 2002.

Bioinformatics: Sequence and Genome Analysis by DW. Mount, Clod Spring Harbor 2002.

Bioinformatics: Genes, Proteins & Computers by C.A. Orengo, Advanced Text 2003.

Bioinformatics: A practical guide to the analysis of genes and proteins by Baxevanis &

Ouellette, Wiley 2005.

TUTORIAL/ PRESENTATION (2 CREDIT HOURS)

SKILL ENHANCEMENT

LEARNING OBJECTIVES: This is a presentation skill enhancement course, which will enable

students to prepare seminar of assigned topics covering recent knowledge in the field. Evaluation

will be based on the quality of the review, oral presentation and comprehension of the subject. The

exact details of the activities will be described by the faculty accordingly.

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MVI-903: RESEARCH SEMINAR. (2 CREDIT HOURS)

PROPOSAL ASSIGNMENTS

LEARNING OBJECTIVES: NCVI will encourage working with clinicians and/other scientists to

develop translational overlaps so the field of investigation could be enhanced. This is a skill

enhancement course, which will enable students to participate in to variety of activities of their

choice. These will include either preparing a review in a given subject or preparing a research

proposal covering recent knowledge in that field. The exact details of the activities will be described

by the faculty time to time accordingly.

MVI-910: STEM CELL STUDIES (3-CREDIT HOURS) This course is designed to educate students about the new concepts of stem cell research. Primary

cell culture techniques, pluripotent cell isolation, dentriatic cells injection techniques will be taught.

COURSE CONTENTS

Hematopiotic stem cell/lineage

Bone marrow transplantation

Embryonic vs. Endodermal lineage.

Mesenchymal vs. Neural lineage

Proliferation markers

Cytokines & growth factor for stem cell culture

In vitro cell tracking (flowcytometry/FACS)

Chemical labeling of proteins and cells

Stable isotope labeling in culture medium

Bead based separation systems

Fluorescent and Colorimetric labeling & detection

HLA-typing

T-cell receptor gene transfer by lentiviral vectors in adoptive cell therapy

Karyotyping.

MVI-920 LAB DIAGNOSTICS/INSTRUMENTATION (3 Credit hours; TO BE OFFERED)

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MVI-930 BIOETHICS/BIOSAFETY (3 Credit hours)

LEARNING OBJECTIVES: This course will be offered into 2 parts. First part is designed to

educate students about work ethics, creating a culture for dedicated research, honesty/scientific

credibility, sexual harassment, clinical misconduct, patients confidently and plagiarism. Second part

will deal with safe handling of key chemicals, solvents, safe acids, base, liquid nitrogen fire, viruses,

bacteria and carcinogens. Proper Instrumental handling to protect oneself in case of accidents

MVI-940: EXPERIMENTAL THERAPEUTICS (3 Credit hours) LEARNING OBJECTIVES: This course is designed to search for the potential approaches that

will arrest the viral-induced pathogenesis and leukemia. The oxidative and anti oxidative signaling

pathways that are perturbed in many viral and immune diseases will be studied.

COURSE CONTENTS: Introduction

Experimental drug design

A virological benefit from an induction/maintenance strategy: the Forte trial

Interferon: cellular executioner

Therapeutic issues in HIV/HCV-coinfected patients

Tumor therapy mediated by lentiviral expression of shBcl-2 and S-TRAIL

Antihyperalgesic effect of a recombinant herpes virus

Silymarin (milk tussle) as potential therapeutics for oxidative stress

Flavinoid and Opinopid compounds as antiviral

Antioxidant therapies for cancer and viral infection (SAM, NAC)

Mucosal immunotherapy for alzheimer's disease with viral vectors

Genetic polymorphisms in the chemokine and chemokine receptors

Transition metal therapies during viral infection (zinc supplementation)

Role of cyclic nucleotide phosphodiesterase inhibitors as novel therapeutic agents for the

treatment of human lymphoid malignancies.

PDE4 inhibitors in primary human chronic lymphocytic leukemia (B-CLL)

AND-34, an SH2 domain-containing protein their role in viral diseases

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Focal adhesion protein p130Cas and role in human breast cancer cells

Hsp90, thioredoxin reductase, and other cytosolic translocation factors as therapeutics

Caspase and protease inhibitors to arrest viral replication

SiRNA/ShRNA and antisense approaches to arrest gene expression

BOOKS RECOMMENDED: (List to be provided later)

MVI-95: PLANT VIRUSES AND DISEASES (3 CREDIT HOURS)

Plant Viruses and their nomenclature

Sugar Cane Viruses

Woody plant virus

Cotton Curl Leaf virus

Other Cash crop viruses

Delivery of foreign genes into plants (Agro bacterium Ti based vs. shotgun approaches)

Plant viruses life cycle

Host interactions

Spread through insect vectors

Possible Antiviral approaches for Plant viruses

Viroids, Virosoids.

RECOMMENDED BOOKS:

Interaction between plant viruses and their vectors by R T Plumb.

Plant viruses and viral diseases volume XIII by Frans.

Identification of plant viruses methods and experiments by Nordun.

Atlas for insects and insects plant viruses by Karl.