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Annotation Update. By: Sandra, Tal, Morella. MarQuardt: A3 Phage Annotated. Genes 1-20 Tal: Genes 1-8 Morella: Genes 9-16 Sandra: Genes 17-20. Areas of Confusion. Is this gene’s coding potential all included?. More Areas of Confusion. - PowerPoint PPT Presentation
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Annotation Update
By: Sandra, Tal, Morella
MarQuardt: A3 Phage Annotated
• Genes 1-20
– Tal: Genes 1-8
–Morella: Genes 9-16
– Sandra: Genes 17-20
Areas of Confusion
• Is this gene’s coding potential all included?
More Areas of Confusion
• It was hard to see which gene was which between DNA Master and the Phamerator Maps
• Gene 1 was confusing as to whether it was actually a gene or not because of all the factors that played in Gene 1 was also difficult because it was the
first gene annotated
Genes Added or Deleted
• No genes were added to our annotated genes
• Gene 1 was deleted
-Lack of start codon
-No Blast match
-Gene is too short
-Coding potential is just a peak
Interesting Features
• There are 3 tRNAs in our section to annotate
• We have not annotated them yet
Interesting features• Gene 5 and 7 had an overlap with the
previous gene, so they were both changed and their SD scores and ORFs were lowered
Gene 5
Gene 7
More Interesting Features
• For the blast match, genes 10-16 match with Vix or Bxz2
• Only gene 9 matches with JHC117 in our sequence annotated
• For the blast match, genes 17-20 all matched with Bxz2
Phamerator SummaryGene Pham Number of Members Cluster
2 6482 101 A1-A10, T, Muddy
3 1843 140 A1-A10
4 2104 53 A3, A4, A7, A10
5 3774 69 A2-A4, A6, A7, A9, A10
6 6505 200A2-A6, A8-A10, B1-B5, F1, I1, I2, K1-K5, L1, L3, M, N, P, Dori, Muddy,
Patience, Sparky-draft
7 2102 26 A2-A4, A10
8 4284 423
A1-A10, B1-B5, C1, D, E, F1, F2, G, H1, H2, I1, I2, J, K1-K5, L1-L3, M, N, O, P Q, R, S, T, DS6A, Moo Moo draft, Muddy, Patience, Sparky-draft,
Wildcat
9 3634 347A1-A5, A7-A8, A10, B1-B4, C1, F1-F2, G, I1, J, K1-K5, L1-L3, M, N, O, P,
Q, R, DS6A, MooMoo-Draft, Patience, Wildcat
10 4144 148 A1-A1, S, T, DS6A, Muddy, Sparky-Draft
11 6487 151 A1-A10, J, S, T, Muddy
12 1839 141 A1-A10
13 1840 95 A1-A10
14 1841 95 A1-A10
15 255 78 A2-A4, A6, A7, A9, A10
16 7 140 A1-A10
17 6504 119 A1-A10, B4, I1. I2, K4, N, P, Dori
18 8 140 A1-A10
19 9 140 A1-A10
20 10 140 A1-A10
Gene 8 Pham Circle
• This map displays that Pham 4284 is found in many different Clusters
Map of Patience and MarQuardt
Questions Remaining
• Coding potential of gene 16
• Genes 17-20 were straightforward and only a few start codons were changed
• The 3 tRNAs need to be annotated