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NATIONAL CONFERENCE
BIOGENESIS – V
“INSIGHTS AND INNOVATIONS IN
BIOTECHNOLOGY”
2nd - 3rd August 2017
Organized by:
Department of Biotechnology
College of Engineering and Technology
IILM Academy of Higher Learning
Greater Noida
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Index
1. About IILM 8
2. About Department of Biotechnology 8
3. About Biogenesis 3
4. Biogenesis V - Insights & Innovations in Biotechnology 9-10
5. Theme of conference 10
6. Messages of eminent personalities 10
7. Message from Vice Chancellor of Jamia Millia Islamia University 11
8. Message from Vice Chancellor of Aligarh Muslim University 12
9. Message from Director of AIIMS, New Delhi. 13
10. Message from Technical Director of the department of Information 14
Technology, National Informatics Center, New Delhi
11. Message from HOD, Toxicology & experimental Medicine, CDRI, 15
Lucknow
12. Message from Deputy Chief Minister, Govt. of NCT of Delhi 16
13. Message from Secretary, Council of Science & Technology UP, Lucknow 17
14. Message from Senior Director, IILM-CET, Greater Noida 18
15. Message from Director (Academics), IILM-CET, Greater Noida 19
16. Message from Convenor of Biogenesis-V, IILM-CET, Greater Noida 20
17. Message from Organizing Secretary of Biogenesis-V, IILM-CET, 21
Greater Noida
18. List of patrons and other committees 22-24
19. Keynote addresses
i. Biotechnology and chemistry interface: a pharmaceutical industry 25-27 Dr. Rakeshwar Bandichhor API R & D, IPDO, Dr. Reddy’s Laboratories Ltd., Bachupally, Hyderabad, Telangana, India 500090
ii. Dr. Ramesh Chandra 28-29
Professor, Department of Chemistry, University Of Delhi, Delhi- 110007
iii. Structural basis of antibacterial action of innate immune proteins and their 30-32 applications as resistance-free Protein-antibiotics Dr. T.P. Singh Biophysicist and Scientist, Department of Biophysics, All India Institute of Medical Sciences, New Delhi
iv. Molecular mechanism of plant derived molecules in neuro-protection 33-34
Dr. Pravir Kumar Associate Professor, Department of Biotechnology Delhi Technological University (Formerly Delhi College of engineering)
v. Natural products from endophytic fungi for pharmaceutical uses 35-36
Dr. Sunil Kumar Deshmukh TERI–Deakin Nano Biotechnology Centre, The Energy and Resources Institute, Darbari Seth Block, IHC Complex, Lodhi Road, New Delhi, India, 110003;
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vi. Phytase of the yeast pichia anomala: Progress and perspectives 37-38
Dr. T. Satyanarayana Division of Biological Sciences & Engineering, Netaji Subhas Institute of Technology (DU)
vii. Importance of Medicinal Plant Diversity and Impact of Degraded 39-40
Environment on Active Constituents Dr. Javed Ahmad , Department of Botany, JamiaHamdard, Delhi
viii. Smart Materials for Stealth Applications 41-42 Dr T C Shami Scientist ‘G’ (Associate Director), Directorate head “Directorate of Stealth Materials” in DMSRDE, Kanpur.
ix. Effect of Radiofrequency Radiation emitted from Cell Phone and Cell 43-44
Phone Tower on human health Dr. Ravindra Nath Div. of RBMCH, Indian Council of Medical Research, New Delhi, India
20. Abstracts 45-86 20.1 TARGETED DRUG DELIVERY 45
Faiz Hashmi, Osama Abdul Mannan and Shubham Rai Department of Biotechnology, IILM -CET, Greater Noida, Uttar Pradesh -201306, India.
20.2 BRAIN COMPUTER INTERFACE: NEW ERA IN THE FIELD 46 OF SCIENCE AND TECHNOLOGY Shalini Singh1, Manisha Kumari1, Dr. Mohammad Zeeshan Najm* Department Biotechnology, NIET, Greater Noida
20.3 ABZYMES 47 Ananya Srivastava Department of Biotechnology, IILM-CET, Greater Noida, Uttar Pradesh -201306, India
20.4 GENETIC SUSCEPTIBILITY OF APOLIPOPROTEIN B GENE 48 VARIANTS TO CORONARY ARTERY DISEASE IN NORTH INDIANS Sumanpreet Kaur1, Navneet Kaur Saini2, Rajesh Vijayvergiya3, Rupinder Tewari1, Jasvinder Singh Bhatti2,4
1Department of Microbial Biotechnology, Panjab University, Chandigarh, India 2Department of Biotechnology, Sri Guru Gobind Singh College, Chandigarh, India 3Department of Cardiology, Postgraduate Institute of Medical Education & Research, Chandigarh, India 4Texas Tech University Health Sciences Center, Lubbock 79430 TX United States
20.5 SCORPION VENOM: A WEAPON TO FIGHT CANCER 49 Ankita Bhattacharya and Monika kesarwani Department of Biotechnology, IILM-CET, Greater Noida,
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Uttar Pradesh -201306, India
20.6 MICROBIOLOGICAL QUALITY ASSESSMENT OF WATER 50 AVAILABLE TO COMMON TRAVELLERS IN DELHI NCR Shyam Sunder, Mohamed Shaffi and J.S. Sandhu FICCI Research and Analysis centre, Dwarka Delhi 110077
20.7 3D PRINTED BLOOD VESSELS 51 Mahima Srivastava , Homa tasnim, Priyanka roy
Department of Biotechnology, IILM-CET, Greater Noida, Uttar Pradesh -201306, India
20.8 MICROBIAL QUALITY ASSESSMENT OF STREET FOOD 52 AT RAILWAY AND METRO STATIONS OF DELHI NCR Arpit Kumar,Vijay Kumar and J.S. Sandhu FICCI Research Analysis Centre Dwarka Delhi 001177
20.9 GLOBALIZATION OF HERBAL HEALTH- (A REVIEW) 53 Nainika Tanwar, Aishwarya Gupta and Sargam Verma Department of Biotechnology, IILM-CET, Greater Noida, Uttar Pradesh -201306, India
20.10 DESIGNING OF POTENTIAL ANTAGONIST OF SRPK1 FOR 54 APPLICATION IN CANCER THERAPY Anshuman Chandra1, Punit Kaur2, Nagendra Singh1, and Imteyaz Qamar1
1School of Biotechnology, Gautam Buddha University, Greater Noida, Gautam Budh Nagar, U.P.201308, India
2Department of Biophysics, All India Institute of Medical Sciences, New Delhi
20.11 BIOSURFACTANTS 55 Pooja choudhary and Ved Prakash
Department of Biotechnology, IILM-CET, Greater Noida, Uttar Pradesh -201306, India
20.12 CHARACTERIZATION OF METAL TOLERANT BACTERIA 56
ISOLATED FROM HEAVY METAL POLLUTED GLASS INDUSTRY FOR ITS ROLE IN BIOREMEDIATION Akash Chaudhary, Ritvesh and Anubha Sharma Amity Institute of Biotechnology, Amity University, Noida, UP, India
20.13 PROTEIN FOLDING INVOLVING MOLECULAR CHAPERONES 57 Priya Rai
Department of Biotechnology, IILM-CET, Greater Noida, Uttar Pradesh -201306, India
20.14 MOLECULAR CHARACTERIZATION OF PHYTOPLASMA 58 ASSOCIATED WITH CATHARANTHUS ROSEUS IN CENTRAL UTTAR PRADESH
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N N Tiwari1, R K Jain2 and A K Tiwari1
UPCSR Shahjahanpur, UP, Anand engineering college , Agra, UP
20.15 CRISPR: THE FUTURE OF DNA EDITING 59 Ramsha Hashmi, Mohita Chugh and Neha Tiwari
Department of Biotechnology, IILM-CET, Greater Noida, Uttar Pradesh -201306, India
20.16 WATER BLOBS: A SUBSTITUTE AND NOVEL APPROACH 60
FOR REMEDIATION OF WASTE GENERATED FROM
POTABLE WATER BOTTLES
Prerit Gupta, Sugandha Katiyar, Dr. Mohammad Zeeshan Najim
Department of Biotechnology,
Noida Institute of Engineering and Technology, Greater Noida
20.17 MOLECULAR TOOLS FOR FORENSIC SCIENCE 61
Riya Marwah and Neha Srivastava Department of Biotechnology, IILM-CET, Greater Noida, UP
20.18 STRUCTURAL AND FUNCTIONAL ROLE OF CONSERVED 62 WATER MOLECULES IN BOVINE LACTOPEROXIDASE ENZYME: A STUDY BY STRUCTURAL BIOINFORMATICS METHOD
Sagar Bashyal1, Shubham Rai1, Hridoy R. Bairagya2 and T.P.Singh2* 1Department of Biotechnology, IILM-AHL CET, Greater Noida-201306 2Department of Biophysics, AIIMS-New Delhi -110029
20.19 APPLICATIONS OF CRISPR - CAS9 63
Sadaf Raza Department of Biotechnology, IILM-CET, Greater Noida, Uttar Pradesh -201306, India
20.20 SYNTHESIS OF SMART NANOVEHICLE AND ITS 64
VARIOUS BIOMEDICAL APPLICATIONS Nisha Yadav,1 Naveen Kumar,2 Seema Sehrawat,2 ,B. Lochab1* 1Department of Chemistry and 2Life Sciences, School of Natural Sciences, Shiv Nadar University, Gautam Buddha Nagar, Uttar Pradesh, 201314, India.
20.21 EBOLA VIRUS DISEASE AND MANAGEMENT 65
Shambhavi Shukla and Pushkar Saw Department of Biotechnology, IILM-CET, Greater Noida, Uttar Pradesh -201306, India
20.22 PHARMACOLOGICAL REVIEW OF CENTELLAASIATICA 66
: A POTENTIAL DRUG Swati Raina, Arpita Roy, ArusheTickoo, Navneeta Bharadvaja* Plant Biotechnology Laboratory, Department Of Biotechnology, Delhi Technological University, New Delhi-110042, India
20.23 DIFFERENTIATING ABILITY OF STEM CELLS 67
Sheetal Gupta and Shalini Singh Department of Biotechnology, IILM-CET, Greater Noida, Uttar Pradesh -201306, India
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20.24 A REVIEW ON PHARMACEUTICALLY IMPORTANT 68 MEDICINAL PLANT TRILLIUM GOVANIUM
Arushe Tickoo, Arpita Roy, Swati Raina, Navneeta Bharadvaja*
Plant Biotechnology Laboratory, Department of Biotechnology,
Delhi Technological University, New Delhi-110042, India
20.25 ALGAE-FUELED BUILDINGS 69
Shimona Chhetry, Rishika Sharma and Neha Nanda Department of Biotechnology, IILM-CET, Greater Noida, Uttar Pradesh -201306, India
20.26 FIGHTING CANCER WITH NANOTECHNOLOGY 70
Yatin Aggarwal and Garima Sharma Department of Biotechnology, IILM-CET, Greater Noida, Uttar Pradesh -201306, India
20.27 EFFECT OF CREATININE ON COGNITIVE PERFORMANCE 71
Yetnesh Dubey and Trisha saha
Department of Biotechnology, IILM-CET, Greater Noida, Uttar Pradesh -201306, India
20.28 SUPERBUGS AND MULTIDRUG RESISTANCE 72 Shalvi Singh and Lubna Zeenat Department of Biotechnology, IILM-CET, Greater Noida, Uttar Pradesh -201306, India
20.29 GENETIC ASPECTS OF ALZHEIMER’S DISEASE 73
Teena Chandna Department of Biotechnology, IILM-CET, Greater Noida, Uttar Pradesh -201306, India
20.30 VERTICAL FARMING 74
Aditi Shrivastava Department of Biotechnology, IILM-CET, Greater Noida, Uttar Pradesh -201306, India
20.31 STEM CELL THERAPY 75
Gunjan Arya Department of Biotechnology, IILM-CET, Greater Noida, Uttar Pradesh -201306, India
20.32 ANGIOGENESIS IN CANCEROUS CELLS 76
Charu Sharma, Nishi Bhaduri and Vaishali Thapa Department of Biotechnology, IILM-CET, Greater Noida, Uttar Pradesh -201306, India
20.33 RHEUMATOID ARITHRITIS AND ITS TREATMENT 77
Kumari Rhaeva
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Department of Biotechnology, IILM-CET, Greater Noida, Uttar Pradesh -201306, India
20.34 ROLE OF DNA FRAGMENTATION IN PREVIOUS 78 IMPLANTATION FAILURES
Sapna Gandhi1 ,Dr. Keshav Malhotra ,Dr Jaideep Malhotra, Dr. Narendra Malhotra, Dr. Neharika Malhotra, Shilpa Marwah,
Chand Mohamad 1Department of Biotechnology, CET IILM AHL, Gr. Noida
20.35 GREEN BIOTECHNOLOGY 79 Shanez Noorie Khan Department of Biotechnology, IILM-CET, Greater Noida, Uttar Pradesh -201306, India
20.36 GOLD NANORODS-KILLING CANCER IN THE HEAT OF 80 MOVEMENT
Vinita kumari, Department of Biotechnology, IILM-CET, Greater Noida, Uttar Pradesh -201306, India
20.37 PONCET DISEASE - 'TUBERCULOUS RHEUMATISM' 81 Nikita Sharma Department of Biotechnology, IILM-CET, Greater Noida, Uttar Pradesh -201306, India
20.38 APPLICATION OF MONOCLONAL ANTIBODY IN THE 82
TREATMENT OF INFLAMMATORY CHRONIC DISEASES Shivangi Tripathi ,Shruti Singh Department of Biotechnology IILM CET Greater Noida, UP
20.39 GENETICALLY MODIFIED ORGANISM (GMO) 83 Rishita De, Nitin Rai 1Department of Biotechnology, CET IILM AHL, Gr. Noida
20.40 DATA MINING: KNOWLEDGE DISCOVERY AND 84 INTERACTIVE DATA MINING IN BIOINFORMTIC AND IT’S APPLICATION Shubhali Pandey[1], Faiz Hashmi[2] , Roma Chandra[3] Department of Biotechnology, IILM CET, Greater Noida, Uttar Pradesh -201306, India
20.41 GENETICALLY MODIFIED ORGANISM INTRODUCTION 85 Anukriti Mishra Department of Biotechnology IILM CET Greater Noida, Uttar Pradesh 201306
20.42 STEM CELLS BASED THERAPY FOR TARGETTING 86
SKIN TO BRAIN CANCER Nikita Sharma Department of Biotechnology,IILM CET Greater Noida, Uttar Pradesh 201306
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Journey of IILM in integrated learning in management was originated in 1993 under the aegis of
the Ram Krishan & Sons Charitable Trust. Today, 24 years after its inception, IILM has been
delivering superior quality education consistently and incessantly. It is this unique heritage
spanning decades of excellence in higher education that sets us apart from other educational
institutes. The IILM ethos is focused on identifying and nurturing the next generation of thought
leaders through intuitive education. IILM’s mix of nationalities, cultural backgrounds, academic
and professional experiences make it one of the most exciting and enriching business schools in
the country. Its long belief and efforts to prepare global managers who have cross cultural
training has become a benchmark for management institutes across the globe.
The IILM Academy of Higher Learning, Greater Noida was set up in 1st April, 2001 under the
aegis of the Ram Krishan & Sons Charitable Trust to groom bright young students, who would
chart the future course of a progressively global society. College of Engineering &Technology,
School of Engineering and Applied Sciences, School of Business Management and College of
Management Studies under the Academy aim at achieving excellence in technological and
management education through adaptation, innovation and continuous up gradation.
Department of Biotechnology is flourishing in IILM Academy of Higher Learning – College of
Engineering and Technology, Greater Noida. The Department holds its roots since 2002 and with
all these years had remarkably worked in the field of education. Walking with the ongoing
scenario of Biotechnology field, the Department of Biotechnology is growing in the field of
education and research. Department of Biotechnology is remarkably working in the field of
Education & Research and is regularly organizing conferences / seminars / guest lectures round
the year promoting innovative ideas in field of Biotechnology.
Gaining knowledge is a never ending process and so is research in the field of Biotechnology.
We focus on learning through practicing and Department of Biotechnology here at IILM is
About IILM
About Department of Biotechnology
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working to give all the practical knowledge through its research laboratories. We are standing
tall in the field of Education with NBA accreditation along with AICTE approval and AKTU
affiliation. Research Laboratories with quality work is our major strength. Multiple career
opportunities giving a new outlook to the career options in biotechnology is introduced to
students focusing on the placement process right from the beginning and ending up every year
with an Excellent Placement Record.
BIOGENESIS is a vision initiated and inculcated by Department of Biotechnology at IILM in
the year 2004, with the objective to bring together inspiring speakers & experts for bridging the
gap between research & industry, discussing innovative ideas, challenges & solutions in the field
of biotechnology.
BIOGENESIS started in 2004 as BIOGENESIS-I with Dr. Kiran Mazumdar Shaw, Chair person,
BIOCON, Bangalore as a Chief Guest.
BIOGENESIS-II was organized in 2009 with Prof. R. P. Bajpayee, Vice Chancellor,
Kurukshetra University as a Chief Guest & Dr. Akhil C. Banerjea, NII as Keynote Speaker.
BIOGENESIS-III was organized in 2014 with Prof. Ravi Mehrota, Director General, ICPO
(ICMR) as Chief Guest.
AICTE Sponsored BIOGENESIS-IV was organized in 2015 with Prof. Ram Shankar Katheria
Hon’ble Minister of State for HRD as a Chief Guest.
Department of Biotechnology is following the legacy by organizing a National Conference
BIOGENESIS - V: “Insights and Innovations in Biotechnology” on 2nd & 3rd August 2017.
BIOGENESIS - V: “Insights and Innovations in Biotechnology” on 2nd & 3rd August 2017.
Experts from leading Biotechnology & Pharmaceutical companies of India like Dr. Reddy’s,
Bharat Biotech, Sun Pharmaceuticals, along with Researchers & Academicians from AIIMS,
IIT-D, ICMR, NESA, JAMIA-HAMDARD, TERI, DRDO, DTU, and NSIT are part of this
conference.
About Biogenesis
Biogenesis V - Insights & Innovations in Biotechnology
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The conference objects to discuss innovations, challenges & opportunities in the field of
biotechnology. It aims to bring together eminent scientists, industry experts, academicians &
researchers. It will provide a platform for young scholars to present their research work and
interact with experts in the field of biotechnology. The conference aims to discuss on current
technological aspects and methodologies used in biotechnology covering thrust areas like
pharmaceutical biotechnology & drug discovery, medical biotechnology, industrial
biotechnology, protein engineering, plant & environmental technologies, microbial research,
food biotechnology. The conference will create awareness about ongoing research with
discussions on practical experiences, innovative ideas and future prospects of research for
academicians & students.
▪ Biotechnology & Applied Biochemistry
▪ Biomass, Bioenergy & Biofuels
▪ Biotechnology & Reproductive Biology
▪ Toxicology & Mycology
▪ Biotechnology & Drug Discovery
▪ Clinical Research & Medical Biotechnology
▪ Genetic Engineering & Transgenic plants
▪ Genomics, Proteomics & Bioinformatics
▪ Interface between Defense and Biotech Research
▪ Microbial Biotechnology
▪ Nano-biotechnology
▪ Pharmaceutical Biotechnology
▪ Plant, Food & Agriculture Biotechnology
Eminent personalities from various research backgrounds have congratulated and sent their
warm wishes in the form of their messages. It’s our pleasure to share those messages here to
encourage all the researchers, academicians and young minds.
Theme of conference
Messages of eminent personalities
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MESSAGE
On the behalf of entire fraternity of Biotechnology Department at IILM
College of Engineering and technology, I extend my warm welcome to
Hon’able chief Guest, Eminent Guest of honor, Distinguished keynote speakers, delegates and
participants of National Conference “Biogenesis V: Insights and Innovations in Biotechnology”
Biogenesis is not just a conference, It's a Tradition initiated and continued forward by
Department of Biotechnology since 2004 to amalgamate academia with research to set up new
dimensions and establish pioneer landmarks in diversified domains of Biotechnology.
Continuing ahead with the convention, Biogenesis-V was initiated to inculcate innovative
research aptitude in young technocrats and foster their entrepreneurial outlook in field of
Biotechnology. Biogenesis-V aims to bring Top researchers, corporate dignitaries and
outstanding Academicians in the domain of Biotechnology on common forum to ignite young
minds of biotechnology towards high quality research in myriad horizons.
I hope this conference will address the evolution, innovations, sophistications, paradigms and
major shifts in pharmaceutical and biotech industries with time. Grey areas and Daedalian issues
emerging with the advancement of pharmaceutical and healthcare will be discussed on this
platform along with futuristic insights in multifarious domains.
I express my heartfelt gratitude to Indian Council of Medical Research (ICMR), PS3 labs and
American Chemists Society (ACS) for extending their support and encouragement in making
“Biogenesis-V:Insights and Innovations in Biotechnology” possible.
I thank my entire team for putting their dedicated endeavors to make this event successful.I pray
to Almighty for smooth conduct and success of this conference.
Ms. Pallavi Singh
Convenor, Biogenesis-V
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MESSAGE
I take this opportunity to extend my warm greetings to all the dignitaries and participants at the
National Conference Biogenesis V – “Insights & Innovations in Biotechnology” organized on 2nd
- 3rd August 2017.The conference is a stupendous effort by the Department of Biotechnology,
IILM CET Greater Noida to bring together the three major contributors - academic institutions,
industries and research organizations.
The conference aims to provide scientific platform to all the participants to congregate and
interact with specialists from the field of biotechnology. The conference covers keynote talks by
experts followed by oral & poster presentations based on the thrust areas of biotechnology.The
conference themes have considered all the major biotechnology fields to give a platform at a
large scale and attract both academic and industrial experts and learners.
I am grateful to the sponsors for their support to make this conference a success. I am very
thankful to our management, seniors and all colleagues for their unstinted help in organizing the
conference.At the same time I am proud to mention the enthusiasm and efforts of students.The
conference is a memorable experience and will be cherished by everyone forever.
Ms. Roma Chandra Organizing Secretary
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PATRONS
Maj. Gen B.D. Wadhwa
Mr. O. P. Sharma
Mr. Ajay Pratap Singh
Dr. Siddartha Sarkar
ADVISORY COMMITTEE
Dr. Krishana Ella (Bharat Biotech International)
Dr. R.K.Singh (CDRI)
Mr. Binu George (ACS)
ADMINISTRATIVE COMMITTEE
Col. Kanakeel Ravi
Mr. Anil Gupta
BIOGENSIS-V
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CONVENOR
Ms. Pallavi Singh
Organizing Secretary
Ms. Roma Chandra
TREASURER
Ms. Garima Gupta
COMMITTEE MEMBERS
Dr. Sanjay Awasthi
Dr. Meenu Singh
Dr. Dhiresh Pathak
Dr. Avijit guha
Dr. Charu Agarwal
Mr. Avinash Singh
Dr. Arpita Mishra
Mr. Vikas gupta
Ms. Neha Srivastava
Mr. Ved Prakash
Ms. Charu Sharma
Ms. Neha Tiwari
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REGISTRATION COMMITTEE
Dr. Charu Agarwal
Ms. Charu Sharma
Ms. Neha Tiwari
SOUVENIR COMMITTEE
Dr. Charu Agarwal
Dr. Arpita Mishra
SCIENTIFIC COMMITTEE
Dr. Charu Agarwal
Dr. Arpita Mishra
VENUE MANAGEMENT
COMMITTEE
Dr. Avijit Guha
Dr. Arpita Mishra
Ms. Neha Tiwari
RECEPTION COMMITTEE
Dr. Sanjay Awasthi
Dr. Charu Agarwal
Dr. Dhiresh Pathak
HOSPITALITY COMMITTEE
Dr. Avijit Guha
Dr. Dhiresh Pathak
CATERING COMMITTEE
Ms. Garima Gupta
Ms. Neha Srivastava
TRANSPORT COMMITTEE
Dr. Dhiresh Pathak
DISCIPLINARY COMMITTEE
Mr. Avinash Singh
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Dr. Rakeshwar Bandichhor
API R & D, IPDO, Dr. Reddy’s Laboratories Ltd.
Bachupally, Hyderabad, Telangana, India 500090
Dr. Rakeshwar Bandichhor is working as a Director, API-R&D at Dr. Reddy’s Laboratories,
Hyderabad, holds a doctorate in Chemistry from University of Lucknow/University of
Regensburg, Germany and worked as Postdoctoral Fellow at University of Regensburg,
Germany, University of Pennsylvania and Texas A&M University.
He has co-authored more than 166 papers including patents and book chapters
published/accepted in various International Journals and contributed to more than 90 academic
national and international conferences. He has won various awards and honors in his career; few
of them are like Chairman Excellence Award 2010, Best Cost Leadership Award 2010,
Anveshan Award 2011, ISCB AWARD OF APPRECIATION FOR INDUSTRY SCIENTIST
2012, Bharat Jyoti Award 2012 , Roll of Honor Award by in Green Chemistry area 2012, Travel
grant award, UK 2013, Green Innovation Award 2013 by IGCW-2013, FRSC 2014, CChem
2014, Vice Chair, ACS-India Chapter (South India Section) onwards 2016, CRSI Council
member onwards 2017.
He was invited as a Chief Guest in RSC-DST sponsored Green Chemistry workshop at
Delhi University, 2011 and instrumental to organize Catalyst 2013 Symposium featuring Noble
Laureate at Dr. Reddy’s. His interview was published in Nature Medicine 2013, 19, 1200-1203,
in Process India (February issue 2014) and Business Standards (March 2014). He has also
contributed in a concept paper “Reengineering Chemistry” by Tata Strategic Management
Group and IGCW-2013.
Recently he has been conferred with FRSC and admitted to CChem by Royal Society of
Chemistry (RSC) from London, UK. He is also an industry advisor to Deccan and North India
section of RSC. Recently, He became six sigma certified white belt, black belt and master black
belt practionor. He has recently been selected as Vice Chair, ACS-India Chapter (South India
Section) onwards 2016.
KEY NOTE ADDRESSES
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Enzymes are proteins capable of converting substrates to products at a higher reaction rates. The
application of enzymes as catalysts in synthetic transformations has various features to design
greener manufacturing processes offering highly stereo-, chemo- and regio-selective products.
These processes have proven potential of being efficient, cost effective, mild (reaction
conditions) and clean.
A number of enzymes are reported for biotransformation however the focus is
increasingly being shifted to protein evolution and metabolic engineering. These recent advances
found various applications in the synthesis of pharmaceutical products particularly active
pharmaceutical ingredients (APIs).
Recently, Merck and Codexis have developed a green synthesis of sitagliptin, the active
ingredient in Januvia, a treatment for type 2 diabetes. This advancement has led to replace
existing process that includes asymmetric hydrogenation of enamine system. The existing
process has found to have significant limitations e.g.suboptimalstereoselectivitythat requires a
crystallization step, and high-pressure hydrogenation> 200 psi which also requires custom made
manufacturing facility and expensive rhodium catalyst.
Transaminase enzymes are known to effect transamination but traditional enzymes did
not yield products with great enantioselectivity. Subsequently, the collaborative efforts helped to
evolve an enzyme that enabled a new manufacturing process.1 The evolved transaminase
hasimproved biocatalytic activity of the transaminase enzyme over 25,000 fold, with no
detectable amounts of the undesiredenantiomer of sitagliptin.This process offers 56 percent
improvement in productivity with the existing equipment, 12 percent overall increase in yield,
and a 19 percent reduction in overall waste generation.
Along this line, we are engaged in identifying enzymes through DE (directed evolution)
which is a biotechnology tool that helps mimic the natural selection to evolve enzymes for
improved targeted activity for instance; a conversion of intermediates towards manufacturing of
high value and lifesaving medicines.
Biotechnology and chemistry interface: a pharmaceutical industry
perspective
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Figure 1: Application of evolved enzyme in chemical transformation
DE biotechnology is being used to obtain enzyme for conversion of ketones to chiral alcohols,
amines, reduction of epimerisable centers to obtain chiral compounds, amidation, esterification
and de-esterification reactions. These transformations are common in the manufacturing of
medicines (Figure 1). The evolved enzymes (biocatalysts) are found to have a broad impact in
multiple aspects of the chemicalindustry.Biocatalysts can offer multiple advantages e.g. product
manufacturing with lower PMI (process mass intensity) and we see it as a paradigm shift that
will pave the way for cost effective sustainable manufacturing of not only medicines but the
materials of various interest. In our endeavour, we developed breakthrough biocatalytic
approaches having commercial viability and high IP (intellectual property) values in the process
of manufacturing of the medicines. I will discuss few case studies of pharmaceutical interest.
References
1. Savile, C. K.; Janey, J. M.; Mundorff, E. C.; Moore, J. C.; Tam, S.; Jarvis, W. R.;
Colbeck, J. C.; Krebber, A.; Fleitz, F. J.; Brands, J.; Devine, P. N.; Huisman, G. W.;
Hughes, G. J.Science2010, 329, 5989.
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Dr. Ramesh Chandra
Professor, Department of Chemistry,
University Of Delhi, Delhi- 110007
Prof. Ramesh Chandra is a distinguished scientist; Fellow of the Royal Society of Chemistry,
London and an outstanding researcher in the field of Biomedical Sciences. He is Professsor of
Chemistry at University of Delhi and Founder Director of Dr. B. R. Ambedkar Center for
Biomedical Research, University of Delhi. He has been Vice-Chancellor, Bundelkhand
University, Jhansi for six years (1999-2005) as well as the President of the Indian Chemical
Society (2004-2006), Member, Planning Commission, Government of U.P., India and Secretary -
Zaheer Science Foundation, New Delhi, Non-Official Director, Rashtriya Ispat Nigam Ltd
(RINL). Professor Chandra shows deep commitment to the cause of higher education and
research and possess in ample measure, quality of dynamic leadership and a vision required for
building academic institutions. Professor Chandra started his research career at the University of
Delhi, thereafter he went to The New York Hospital-Cornell University Medical Center and the
Rockefeller University, New York; State University of New York at Stonybrook as Assistant
Research Professor. He conducted advanced research at the Harvard University Medical School-
Massachusetts General Hospital, jointly at MIT, Cambridge, USA.
Over the last 38 years, Professor Chandra has contributed largely in the field of Chemical
Sciences and particularly in New Drug Discovery and Development as well as Drug Metabolism.
His research work is being used in the development of drugs for Physiological Jaundice/
Neonatal Jaundice and development of naturally occurring chemotherapeutic agents for the
treatment of Breast and Ovarian Cancers, and drugs for Diabetes and Hypertension. He has
supervised large number of Ph. D. and M. Phil. Students, in all trained more than 100 research
scholars, who are now occupying high positions internationally. He has published more than 250
original Scientific Research Papers in International journals of repute, including Review
Articles/Monographs, Book Chapters; Four of his internationally acclaimed Books.
Prof. Chandra is the recipient of several professional national/ international recognitions.
These includes Award of the Highest Honor of Soka University, Tokyo, Japan (2000); J William
Fulbright Scholarship(1993); The Rockefeller Foundation USA-Biotechnology Career Award
(1993); UGC Career Award(1993); UGC Research Scientist Award (1988); Rajib Goyal Award
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Biogenesis-V
for Young Scientists (2002); Lifetime Achievement Award of The Indian Chemical Society
(ICS) (2003); Bronze Medal of the Chemical Research Society of India (CRSI) (2004); Prof.
Ghanshyam Srivastava Commemoration Award of ICS (2002); Prof. D. P. Chakraborty
Commemoration Award of ICS (2001); IMNM-99 Award; Gold Medal in Integrated Medicine
for New Millennium (1999); Vidya Ratan Gold Medal (2005) and Dr. BR Ambedkar National
Award (2004). He is Fellow of The Royal Society of Chemistry, London; International Academy
of Physical Sciences; Institution of Chemists, India and the Indian Chemical Society. He is
member of several International Scientific Societies.
He has been a member of the Governing Council, BOG, Executive/ Academic Councils
of several Universities/ Institutions globally and also Member – U.P Council of Higher
Education; U.P. State, Youth Welfare Council Member and others. He has also been Consultant
and Advisor to the various multinational companies like Polaroid Corporation, Diakron
Pharmaceuticals, USA, HIKMA Pharmaceuticals-Jordan and Director of BIZ SHAKTI, India
etc; Advisor to various academic institutions and also non-official Director of PSU’s, Govt. of
India. Prof. Chandra is a prolific writer and displays extraordinary flair for writing on themes
particularly to Higher Education and social issues.
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Biogenesis-V
Dr. T.P. Singh
Biophysicist and Scientist
Department of Biophysics,
All India Institute of Medical Sciences,
New Delhi
Dr. T. P. Singh is a renowned biophysicist and scientist known nationally and across the globe
for his work in the fields of Rational Structure-based drug design, Protein Structure biology and
X-ray crystallography. Dr. Singh is a Fellow of the Third World Academy of Sciences, Indian
National Science Academy, Indian academy of Sciences, Alexander von Humboldt
Foundation Biotech Research Society of India.
He was Dean and the Head of Department of Biophysics at AIIMS from 1984-2010.
He has won several national and international honors which includes President of the Indian
Biophysical Society (2014), the Professor D.M. Bose Memorial Award of the Bose Institute,
Kolkata (2013), Thathachari Memorial Science Award for Science and Technology (2012),
Goyal Prize for Life Sciences, Distinguished Biotechnology Research Professor (DBT) (2009),
GN Ramachandran Gold Medal for excellence in Science and Technology (CSIR)
(2006) ,Alexander von Humboldt Fellow (1977), Canadian development Agency Award (1999).
Prof. T.P. Singh has authored more than 250 research publications which have been
published in high impact research journals.
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Biogenesis-V
Considering the alarming rise in the incidence of bacterial resistance to known antibiotics, there
is a desperate need to develop bacterial resistance-free antibiotics. The proteins of the innate
immune system provide the first line of defense against infecting microbes. These proteins
recognize the conserved motifs that are present on the cell walls of bacteria. Thus the success of
the innate immune system depends on the affinity of the proteins of innate immune system
towards the bacterial cell wall molecules. The conserved motifs of microbial cell walls are
called pathogen associated molecular patterns (PAMPs) that include the well known
peptidoglycans (PGN) and lipopolysaccharides (LPS) of Gram-negative bacteria, PGN and
lipoteichoic acid (LTA) of the Gram-positive bacteria and mycolic acid (MA) and other fatty
acids of Mycobacterium tuberculosis.
These PAMPs are classified into two groups: (i) those which contain glycan moieties
such PGN, LPS, LTA etc. and (ii) those that are derivatives of fatty acids such as MA. Therefore,
there should be two independent binding sites for the two different types of PAMPs. The PAMPs
are specifically recognized by innate immunity molecules which are historically known as
peptidoglycan recognition proteins (PGRPs).These proteins bind to PAMPs with significant
affinities and neutralize the infecting pathogens through a variety of actions. There are four types
of PGRPs in mammals including humans, PGRP-L (MW = 90kDa), PGRP-Iα and Iβ (MW =
45kDa) and PGRP-S (MW = 21kDa). PGRP-S represents the domain that has the binding site for
PAMPs. The binding affinities of PGRP-S and structures of unbound and bound PGRP-S from
various species showed that the protein from camel has considerably higher affinity than those of
other animals including humans. The epidemiological data indicate that the camels have the
lowest rates of infections.
Structurally, PGRP-S from camel exists in the form of a dimer whereas the human
protein acts as a monomer. There are only a few sequence differences in the proteins from two
species which are responsible for dimerization of camel protein. As a result of dimerization, a
Structural basis of antibacterial action of innate immune proteins
and their applications as resistance-free Protein-antibiotics
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Biogenesis-V
deep binding cleft is formed in the camel protein whereas only a shallow cleft is present in the
case of human monomeric protein. Because of dimerization, the potency of camel protein is
much higher than the same protein from other species. Thus if camel protein is used or a suitably
mutated human protein is prepared and used, the fight against bacterial infection will improve.
The mechanism of action of PGRP-S involves an effective sequestration of bacteria which
results in the killing of bacteria. Since PGRP-S interacts with bacterial cell wall, the kinetics of
bacterial cell death appears to be similar to those antibiotics which inhibit the biosynthesis of
PGN. Due to this similarity, PGRP-S is suggested to be termed as “protein antibiotics” and
since they bind to bacterial cell wall molecules the issues of side effects and resistance will not
arise and if the potencies are high, the invading bacteria can be tackled rapidly.
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Biogenesis-V
Dr. Pravir Kumar, PhD (Germany)
Associate Professor, Department of Biotechnology
Delhi Technological University (Formerly Delhi College of engineering)
Dr. Pravir Kumar is an Associate Professor since 2012 in the Department of Biotechnology at
DTU and he served as an Associate Professor (Biosciences) and Assistant Director (Center) at
VIT University, Vellore since 2009. He has obtained MS degree from BHU, Varanasi and PhD
degree from Germany in Cardiovascular Physiology. Before returning to India, he has spent
several years in the Neurology Department at Tufts University School of Medicine, Boston, USA
as a postdoctoral fellow and later at faculty position. Until April 2016, he was holding an adjunct
Faculty status in the Neurology Department at Tufts University School of Medicine (TUSM
2009-2016).
His areas of research interest and expertise include molecular chaperone and ubiquitin E3
ligase in neurodegenerative disorders along with the aberrant cell cycle re-entry into aged
neurons and muscles. He is serving as an editorial/reviewer board member for more than 30
journals including Journal of Alzheimer’s disease, International Journal of Neurology Research,
American Journal of Research Communication and reviewers of several leading Elsevier,
Springer, BMC, Bentham, Oxford and many reputed journals. He has published more than 50
papers in peer reviewed journals. Further, he has presented several invited talks and presented
more than 85 papers in international symposium and proceedings.
Until now, he has guided 3 PhD students (single supervision, degree awarded), 32
M.Tech and more than 30 students at MS and BS levels. Currently, he is guiding five PhD
students and many M.Tech and B.Tech students. He is also the member of many national-level
selection committees, including prestigious USIEF Nehru Fulbright program.
He has successfully completed LSRB-DRDO funded research defense project on hypoxia
induced neuro degeneration in India. He is a life and regular member of various professional
societies across the globe.
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Biogenesis-V
Adverse physiological and pharmacological conditions are responsible for the progression of
Alzheimer’s disease (AD) and Parkinson’s disease (PD). These stressors are, heat stress, cold
stress, free radical generation, alteration in mitochondrial dynamics, protein dyshomeostasis, ER
stress, hypoxia, environmental toxins, loss of function of proteins and genetic factors. These
stress situations significantly affect the brain and its related physiological functions.
Moreover, lack of oxygen slows the reflex action, weakness of muscle and cognitive
impairments while environmental toxins impair complex IV activity and hamper electron
transport system. In order to understand the mechanism behind stress induced neuro
degeneration, we generated animal model sand checked the differential expression of various
protective proteins, stress proteins and ubiquitin E3 ligases.
We checked the efficacy of various biomolecules in vivo and confirmed a marked
reduction in the symptoms in neurodegenerative disorders. Taken together, we confirmed that
many screened biomolecules can reverse the effect of neuro degeneration.
References:
1. Jha et al., BiochimBiophysActa.2016 Jun 21.pii: S0925-4439(16)30157
2. Sharma et al., BiochimBiophysActa. 2016 Sep 14;1863(1):324-336
3. Jha et al., J Alzheimers Dis. 2016 Sep 23.
4. Jha et al., J Alzheimers Dis. 2015;48(4):891-917.
5. Kumar et al., J Alzheimers Dis. 2015;43(2):341-61.
6. Kwon and Kumar et al., Hum Mol Genet. 2014 Jul 15;23(14):3681-94.
7. Kumar et al., J Neurochem. 2012 Feb;120(3):350-70.
8. Kumar et al., Hum Mol Genet. 2007 Apr 1;16(7):848-64.
9. Angeline et al., Neuroscience. 2013 Dec 19;254:379-94.
10. Angeline et al., Neuroscience. 2012 Sep 18;220:291-301.
11. Sarkar et al., Brain Res. 2012 Oct 24;1481:59-70.
Molecular mechanism of plant derived molecules in neuro-protection
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Biogenesis-V
Dr. Sunil Kumar Deshmukh
TERI–Deakin Nano Biotechnology Centre, The Energy and Resources Institute, Darbari Seth Block, IHC Complex, Lodhi Road, New Delhi, India, 110003;
Dr. Sunil Kumar Deshmukh received his Ph.D. degree in Mycology with the Professor S.C.
Agrawal at Saugar University Sagar, MP, India in the year 1983. Dr Deshmukh is veteran
industrial mycologist who has spent a substantial part of his career in Hoechst Marion Roussel
Limited (now Sanofi India Limited), Mumbai and Piramal Enterprises Limited Mumbai in drug
discovery. His core expertise is in discovery of antifungal, anticancer, anti-inflammatory and
antidiabetic compounds which is demonstrated in his patents and publications.
He has to his credit 8 patents, 100 publications, and 8 books on various aspects of Fungi
and natural products of microbial origin. The generating diverse microbial metabolite extract’s
library for fast track lead generation is a significant contribution of Dr. Deshmukh. This work
was presented at 6th Annual BioPharma Asia Convention, 2013, Singapore on March 18-21 as an
invited speaker. He is a Fellow of Association of Biotechnologist and Pharmacy, Mycological
Society of India Society for Applied Biotechnology. He has been an executive member of the
Mycological Society of India (2002-2006), Member of editorial board, Kavaka (2007-2009)
(2014-2017) and the Vice President of Mycological Society of India (2010).
Presently, He is President of Mycological Society of India. He has delivered the
prestigious Shome Memorial Lecture at the 31st annual meeting of Mycological society of India
held at Chennai on 29-30th Jan, 2009. He is a peer reviewer for various Journals like
Mycopathologia, Mycoses, Natural Product Research, etc. and guest editor Open Mycology
Journal. He has been a Member of the Board of Studies in various universities.
Currently he is working as Fellow & Area Convenor, Nano Biotechnology Centre, TERI
working towards the development of PGPRs, biofertilizers, crop protective agents, biopesticides
and herbicides through nanotechnology intervention
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Biogenesis-V
Endophytic fungi are defined as fungi that live asymptomatically within the tissue of higher
plants. These fungi came in limelight after the discovery of taxol followed by compounds like
camptothecin, vincristin and cryptocandin.
The focus of our research activity is on the discovery of bioactive metabolites from fungi
from the Indian subcontinent. Our main focus is on endophytic fungi from Indian subcontinent
which offers a great opportunity to discover unexplored fungi with pharmaceutical potential.
India is blessed with two hotspots of biodiversity, out of the total twelve mega biodiversity
centers in the world. Moreover, out of 17,500 flowering plants available in India, over one-third
of these are endemic. These plants enjoy diverse habitats in different geographical regions and
climatic zones ranging from tropical to alpine regions (Himalayas) and from coastal regions to
the deserts.
During the course of our study related to drug discovery using various cell based, target
based and enzyme based screening to get anti-cancer, anti-inflammatory and anti-microbial
compounds; we have screened more than 6000 endophytic fungi isolated from plants of diverse
habitats with medicinal properties to get bioactive molecules. The most prolific producer of
biologically active compounds were the species of Mycoleptodiscus, Phoma, Phomopsis,
Xylaria, Chaetomium, Alternaria, Nigrospora, Curvularia, Fusarium, Arthrinium and sterile
forms which yielded compounds like PM181110, Heptelidic acid, Altersolanol A, Ophiobolin,
Nectripyrone, Ergoflavin, A52688 antibiotic complex, Arthrichitin and Herbarin. An overview of
such compounds and some aspects of fast track lead generation will be covered in this
presentation.
Natural products from endophytic fungi for pharmaceutical uses
37
Biogenesis-V
Dr. T. Satyanarayana
Division of Biological Sciences & Engineering,
Netaji Subhas Institute of Technology (DU)
After Ph.D in 1978, Prof. T. Satyanarayana had post-doctoral stints at Paul Sabatier University
and Institute of Applied Sciences, Toulouse (France). In 1988, he joined the Department of
Microbiology, University of Delhi South Campus as Associate Professor and became Professor
in 1998. His research efforts have been focused on understanding the diversity of extremophilic
fungi and bacteria, their enzymes and potential applications, heterotrophic carbon sequestration,
metagenomics, and heterologous expression of yeast, thermophilic fungal and bacterial genes
encoding industrial enzymes.
He has published over 250 scientific papers and reviews and edited seven books, and has
three patents to his credit. He is a recipient of Dr. G.B. Manjrekar award of AMI in 2003, Dr.
V.S. Agnihotrudu Memorial award of MSI in 2009 and Malaviya Memorial award of BRSI in
2012 for his distinguished contributions. He completed several major research projects.
He is a fellow of National Academy of Agricultural Sciences, Association of
Microbiologists of India, Mycological Society of India, Biotech Research Society (India) and
A.P. Academy of Sciences. During over 40 years of research and teaching, he has mentored 30
scholars for Ph.D. He visited several countries including France, England, Germany, New
Zealand, Australia, Japan, Norway, Sweden, China and others.
He had been the president of Association of Microbiologists of India (2015-16) and
Mycological Society of India (2014-2015). He organized two international and one national
conferences and attended several conferences in India and abroad.
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Biogenesis-V
Phytic acid (myo-inositol hexakis dihydrogenphosphate) is the major storage form of phosphorus
in plants and plant products like cereal grains, legumes and oilseeds that accounts for 50-90% of
the total phosphorus. The salts of phytic acid (phytates) are poorly utilized by monogastrics
(humans, pig, poultry, and fish) due to lack of adequate levels of phytase in their gastrointestinal
tract, thus, acts as anti-nutritional factor in more than one ways, and their excess in the
environment leads to aquatic phosphorus pollution problems. The reduction of phytic acid
content in foods and feeds by enzymatic hydrolysis using phytase is, therefore, desirable.
Phytases are phosphohydrolases that catalyze the sequential release of phosphate from phytic
acid forming myo-inositol and phosphate esters. A major portion of phytase in the yeast Pichia
anomala is cell wall associated, and it is located in the space between cell wall and plasma
membrane. The productivity of cell-bound phytase was higher in fed-batch fermentation in air-
lift bioreactor than that in stirred tank bioreactor. The yeast cells permeabilized with Triton X-
100 showed higher phytase activity than the intact cells. In the cell-bound form, it is optimally
active at 75 C. The purified phytase is a homohexamer with a molecular mass of 384 kD. The
enzyme is optimally active at 60 oC and pH 4.0, and exhibited broad substrate specificity,
thermostability, acid-stability and protease insensitivity. The phytase gene has been cloned and
expressed in Hansenula polymorpha, and its 3-D structure has been proposed. Our efforts in
cloning and expressing the phytase in Pichia pastoris and a marked increase in production level
attained will be discussed. The supplementation of feed of broiler chicks with the yeast biomass
resulted in better growth and phosphorus retention in the chicks, and reduced excretion of
phosphorus in the droppings. The yeast biomass containing phytase also promoted the growth of
fresh water fish Labeo rohita and marine fish Chanas chanas and improved phosphorus
assimilation and decreased phosphorus and ammonia excretion. The intact as well as
permeabilized yeast cells have been shown to be useful in dephytinizing soymilk and
fractionating allergenic protein conglycinin from soymilk, with concomitant liberation of soluble
inorganic phosphate. When wheat dough was supplanted with the recombinant phytase, there
was reduction in phytate with concomitant increase in soluble inorganic phosphate.
Phytase of the yeast pichia anomala: Progress and perspectives
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Biogenesis-V
Dr. Javed Ahmad
Professor, Department of Botany, Jamia Hamdard &
President, National Environment Science Academy, Delhi
Prof. Ahmad was born on January 1, 1951 at Kandhla in the Muzaffarnagar district of Uttar
Pradesh. Dr. Javed Ahmad obtained his Ph.D. in Botany at the Aligarh Muslim University in
1986. He joined Institute of History of Medicine and Medical Research (now Faculty of
Science,Jamia Hamdard) New Delhi in 1975. He participated in the First World Congress on
Oriental Medicine & Yoga held at Bangkok, Thailand (1988) and in the International
Symposium on Liver Diseases held at Hamdard University, Karachi, Pakistan (1993). He was the
Assistant Proctor (1996-97), the Provost, Halls of Residence (1997-98) and the Proctor (2014-
2015) at Jamia Hamdard.
He has more than 70 publications to his credit, most of them pertaining to
pharmacognosy, plant anatomy, ethnobotany and the history of medicinal plants. He has also
worked as Associate Professor for one year (2001-2002) in Toyama Medical & Pharmaceutical
University, Toyama, Japan. He had acted as Head & Professor in the Department of Botany,
Faculty of Science, Jamia Hamdard, New Delhi, 110062. He also acted as Dean, Faculty of
Science, w.e.f. January 2014 – December 2015. He has experience of ten years as Professor of
Botany in his career.
Presently after his retirement w.e.f. January, 2016 he is performing his duties as President
of National Environmental Science Academy (NESA). Dr. Ahmad has done pharmacognosy of
bark and leaf of some Indian medicinal plants like Eugenia jambolana, Ficus benjamina, so as to
ensure authentic identification of crude herbal drugs and distinguish them from adulterants.
Anatomical analysis of samples sold as Unani drug "Bed Sadah" revealed that the leaves of Salix
alba are adulterated by the leaves of Eucalyptus globulus, as identified on the basis of structure
of hypodermis, mesophyll, stomata and trichomes.
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Biogenesis-V
The significance of medicinal plant diversity is undoubtedly undebatable. Currently, the
knowledge and use of medicinal plants for curing of diseases are being displaced by synthetic
drugs. However, natural medicinal plants have great cultural and economic values for global
human populations from the prospective of traditional or indigenous knowledge and uses dates
back to ancient times. The uses and significance of medicinal plants have been reported from
ancient civilizations. Meanwhile, chemical based drugs were discovered in the 18th century.
Medicinal plants are those plant species with specific compounds that contribute in the specific
biological activity of many parts of the plant. Varied compounds are present in different
concentrations in the roots, stems, leaves, flowers, fruits and seeds, which are procured from the
specific plants through different time tested traditional or indigenous techniques around the globe
by several indigenous or aboriginal communities. A wide variety of medicinal plants are in use
today for their therapeutic actions. These medicinal plants are also the raw material for the
pharmaceutical industries.
Many of these degrade quite naturally in the polluted environment and have detrimental
impacts on the ecosystem from a long term ecological health perspective. These are mostly toxic
components containing arsenic, lead, copper, chromium, cadmium, mercury and zinc, etc. Impact
of pesticides on medicinal plant diversity is yet to be studied.
The other anthropogenic activities such as industrialization, urbanization, deforestation,
habitat loss, agriculture malpractice, change in land use pattern, emission of green house gases
(climate change] are major threats affecting phytodiversity of the country. More research is
needed to understand mechanism and extent of these threats on medicinal plant biodiversity.
Recent biotechnological innovations are constantly advancing to help improve our plant diversity
and environment.
Importance of Medicinal Plant Diversity and Impact of Degraded
Environment on Active Constituents
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Biogenesis-V
Dr T C Shami,
PhD, FRSC
DMSRDE, DRDO, GT Road, Kanpur-208013
Dr.T.C.Shami obtained Master’s and Doctorate in Chemistry from CCS University Meerut under
supervision of Prof. G N Mathur (Ex- Director, DMSRDE and Professor, Penn State University,
USA), Obtained FRSC (fellow of Royal Society of Chemistry form Cambridge Universtiy UK,
2016).
His research interests are in the field of nano materials, conducting, magnetic, polymeric
& carbon materials (CNT & Carbon micro-coil), green chemistry LASER and low emissivity
materials & fabric based camouflage products for stealth application in defence. His
recognition/expertise is known as Stealth Material Person.
Dr. Shami has worked as visiting scientist in Penn State University, University of
Arkansas and California University, USA. He has been awarded as an “Excellent Scientist” by
Penn State University-2002. “Outstanding research award” from University of Arkansas, USA-
2007, “Technology Group Award” as a team leader -2010 and other cash awards & also best
performance award- as Rajbhasha Adhikari- 2013&14. Best paper award for Hindi 2016.
He is a member of recruitment board of scientists/assistants in DRDO & various
technical posts in IIT Kanpur. Dr. Shami is reviewer and supervisor of M.Tech and PhD thesis.
He has reviewed more than 15 projects for different IITs, CSIR laboratories & other engineering
and academic institutes costing more than 7 crores. He is a member of different professional
societies & member of advisory board of Dr APJ Abdul Kalam Technical University, Lucknow
and Member of Board of Governors for UPTTI Kanpur.
He had delivered lectures in more than 30 training courses of DRDO. He has published
more than 90 papers in National/International journals & conferences; 43 invited talks; he has
chaired more than 10 international conferences; filed 4 patents & written 02 book chapters.
Currently Dr Shami is working as Scientist ‘G’ (Associate Director), Directorate head
“Directorate of Stealth Materials” in DMSRDE, Kanpur.
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Biogenesis-V
With the advancement in the field of electronics and radar technology, Stealth Technology has
been an important area, especially in the defence sector. Stealth technology has also significant
application in civilian sector, eg. EMI, EMC and aerospace etc., with the rapid growth in the area
of smart nano-materials, frequency selective surfaces and metamaterials. These materials paved
the way for stealth technology. As it encompasses cohesive and integrated efforts of the smart
materials and technologies which are used for ground based, air-bornenaval applications.
Based on the requirement for various platforms, various types of materials and sensors are
used for countermeasure and counter countermeasure techniques. Various type of dielectric and
magnetic materials have been synthesized e.g: conducting materials- polyaniline in the form of
powder, nanotubes, tubular, encapsulation of polyaniline inside different polymeric
microballoons,CNT encapsulated inside the polymeric microballoon, magnetic materials-NiZn
ferrite, MnZn ferrite, Barium hexaferrite, high permittivity materials in the form of powders,
flakes and fibers of BT and BST have been used for the development of radar absorbing coatings
and composites for stealth applications.
Furthermore radar and microwave measurement techniques have also been covered. In this
extensive and informative talk, various aspect of stealth technology is covered in detail which is
particularly used for defense applications.
Smart Materials for Stealth Applications
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Biogenesis-V
Dr. Ravindra Nath,
Div. of RBMCH,
Indian Council of Medical Research, New Delhi, India
Dr. Ravindra Nath, has specialization in biochemistry and molecular genetics. He is actively
involved in conducting multi disciplinary, prospective cohort study on effect of non-ionizing
electromagnetic field on human health.
He has also contributed significantly in the scientific process of epidemiological study
including posing a hypothesis; identifying questions that need answers, designing study
methodology, developing desire perform (questionnaire), collecting data/ information from the
enrolled subjects, co-ordination of prospective epidemiological study and analyzing the data etc.
He is actively involved in various activities of the division such as clinical trial of various
phases of RISUG, systematic review & meta analysis of existing literature on adverse effect of
radiofrequency radiation on health (on both human and animal), use and effectiveness of
Letrozol in human, organizing scientific meetings (like as project review group, experts
committee, scientific advisory committee meeting).
He has participated in up to 30 national and international conferences & seminar delivered
invited lectures and presented poster. He has published about 15 research papers and chapters in
books / conference proceedings and 10 paper/review/meta analysis are communicated. He is a
life member of the Indian Society for the Study of Reproduction & Fertility (ISSRF), Bio-
Electromagnetic Society of India (BESI) and Society for plant Biochemistry and Biotechnology.
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Biogenesis-V
Mobile phone has become a crucial and multipurpose useful device among people of all
strata. In India mobile phone subscribers have reached about 1180.82 million (May, 2017).
The widespread use of mobile phone has resulted in drastically increase exposure of
radiofrequency radiation (RFR) ranging from 0.8GHz to 2.4GHz frequency. The RFR
emitted from cell phone, cell phone towers and from other sources is new threat in the
environment and hence needs to be considered as environmental pollutant. The WHO has
categorized EMF radiation as 2B carcinogen. Impact of RFR on biological effect & health
hazard has been demonstrated by various animal and in-vitro International & National
studies.
These studies reported a significant increase/decrease in the levels of bio markers and
biological response. Biological effects are measurable response to stimulus or to change in
the environment and are not necessarily harmful to our health. Health hazards are changes
that irreversible and stress the system for long period of time. The exposure of RFR has also
been reported to generate excessive reactive oxygen species resulting in gradual increase in
the concentration of free radicals and their accumulation in the body. These Free radicals
adversely alter the structure and functions of bio-molecules in the cell (lipids, proteins, and
DNA) resulting in triggering alternation in regulation of different physiological conditions or
pathological states and hence produces a number of disorders and diseases viz. cardiological,
neurodegenerative, aging, autoimmune, genetic, cancer, reproductive diseases etc.
Though the review of International Scientific data available so far could not establish
conclusive evidence on adverse effects or risk of RFR emitted from cell phone; but growing
body of scientific evidences indicate towards the bio-effects of RFR which may be possible if
not probable. However, the review of studies published from India has established that the
RFR emitted from cell phone and cell phone tower induces biological effects. But it is not
clear whether and when these biological effects will result into health hazards. To address
this issue, ICMR is conducting a multi disciplinary prospective cohort study in Delhi.
Effect of Radiofrequency Radiation emitted from Cell Phone
and Cell Phone Tower on human health
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Biogenesis-V
Faiz Hashmi, Osama Abdul Mannan and Shubham Rai
Department of Bio-Technology, College Of Engineering and Technology IILM Academy of
Higher Learning, Greater Noida, Uttar Pradesh -201306, India.
Email Id: [email protected]
TARGETED DRUG DELIVERY
Targeted drug delivery system is a special form of drug delivery system where the medicament is
selectively targeted or delivered only to its site of action or absorption and not to the non-target
organs or tissues or cells increasing the concentration of medicine in some part of body relative
to others improving efficacy and reduce side effects. Drug may be delivered to the capillary bed
of the active sites, to the specific
type of cells or even an intracellular region (tumor cells but not to the normal cells), to a specific
organ or tissues by complexion with the carrier that recognizes the target.
In treatment or prevention of diseases, pharmaceutical drug instability in conventional dosage
from solubility, biopharmaceutical low absorption, high membrane bounding, biological
instability, pharmacokinetics, pharmacodynamics short half-life, large volume of distribution,
low specificity, clinical, low therapeutic index; are such reason for which necessity of targeted
drug delivery thus required. Present review includes the mechanism, strategies and recent
advances in the field of targeted drug delivery.
Keywords: Strategies of drug targeting, types of drug delivery system, nanoparticles, quantum
electrodynamics.
ABSTRACTS
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Biogenesis-V
Shalini Singh1, Manisha Kumari1, Dr. Mohammad Zeeshan Najm*
Department Biotechnology, NIET, Greater Noida
Presenting Author: [email protected]
Co-Author: [email protected]
Corresponding Author: [email protected]
BRAIN COMPUTER INTERFACE: NEW ERA IN THE FIELD OF
SCIENCE AND TECHNOLOGY
Human brain works on electrical signals transmitting all over the body to send the information in
order to operate body part. Even while rotating eye ball, body increases or decrease the
resistance near eye area. This variation in electric signals can be measured using electrodes or the
myoelectric sensors. So, by implementing these signal processor, we can interface different
devices to control on demand. Hence the main objective is to design and control computer and
different hard ware system using brain waves electric signals. In a nutshell, BCI (Brain
Computer Interface) is a communication path way between the brain and the external peripheral
devices like computers. By using these waves, we can control various home appliances. The
entire concepts consist of four main stages (a) detection (b) amplification (c) processing (d)
output.
Proposed system could help in detecting the variation in electric signal strength through voltage
level near eye area and generates a wireless radio frequency signals in order to control the
robotic prototype model. The technology if applied successfully, would simplify the physical
work of the Human beings and also would play a major role in patient management in
healthcare.
Keywords: Myoelectric, Brain Computer Interface, Robotic Prototype
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Biogenesis-V
Ananya Srivastava
Department of Biotechnology, IILM-CET, Greater Noida,
Uttar Pradesh -201306, India
E-mail address: [email protected]
ABZYMES
An abzyme (from antibody and enzyme), also called as catmab (catalytic monoclonal antibody),
or catalytic antibody, is a monoclonal antibody with catalytic activity. The binding of an
antibody is similar in many ways to that of enzyme-substrate binding. The only difference is that
antigen antibody interaction does not alter the antigen, while enzyme catalyzes a chemical
change in its substrate. However, like enzymes, antibodies of appropriate specificity can stabilize
transition state of a bound substrate, thus reducing the activation energy for chemical
modification of the substrate. Abzymes are usually raised in lab animals immunized against
synthetic haptens. Catalytic antibodies have emerged as powerful tools for the chemical
biologist, enabling the design and realization of specific catalysis for a wide range of chemical
reactions. Abzymes are being used in treatment of HIV.The abzyme does more than bind to the
site, it catalytically destroys the site, making the virus inert, and then can attack other HIV
viruses. A single abzyme molecule has potential to destroy thousands of HIV viruses. The study
of catalytic antibodies as a whole has vastly increased current understanding of the mechanisms
of enzyme catalysis and represents another step forward in the attempts to create artificially
engineered biological enzymes.
Keywords: Transition state;Haptens; Superantigenic region; HIV virus Cell-mediated
immunity.
48
Biogenesis-V
Sumanpreet Kaur1, Navneet Kaur Saini2, Rajesh Vijayvergiya3, Rupinder Tewari1,
Jasvinder Singh Bhatti2,4 1Department of Microbial Biotechnology, Panjab University, Chandigarh, India 2Department of Biotechnology, Sri Guru Gobind Singh College, Chandigarh, India 3Department of Cardiology, Postgraduate Institute of Medical Education & Research,
Chandigarh, India 4Texas Tech University Health Sciences Center, Lubbock 79430 TX United States
GENETIC SUSCEPTIBILITY OF APOLIPOPROTEIN B GENE VARIANTS TO
CORONARY ARTERY DISEASE IN NORTH INDIANS
Coronary artery disease (CAD) is a multifactorial syndrome that has become a major health
problem in many countries. It is considered to be a consequence of the relationship between
genetic and environmental factors. The aim of present study was to investigate the association of
EcoRI polymorphism of apolipoprotein B (ApoB) gene with the risk of CAD in North Indians. A
total of 410 CAD patients and 406 controls were recruited, and medical history and
anthropometric measurements were taken. All the clinical parameters including total cholesterol
(TC), triglycerides (TG), HDL, LDL, VLDL, and glucose were done to correlate the relation
among the EcoRI genotypes. The ApoB gene was amplified using the PCR-RFLP method. In
CAD patients the total cholesterol, triglycerides and LDL-cholesterol was higher in R+R-
genotype than R+R+ genotype but values were non-significant. Results show no significant
differences in the distribution of Apo B genotypes (R+R+, R+R- and R-R-) among the controls
and CAD patients. The frequency of R+ allele was higher than R- alleles in both CAD and
control subjects. The results for homozygous mutant alleles R-R- were not observed in control
subjects. So the test for association was not defined for this genotype. Logistic regression
analysis of data did not show any significant association of R+R- genotype against CAD
development in our study population (OR = 0.896; 95% C.I. = 0.65-1.22; p=0.522). The BMI,
WHR and body fat percentage did not differ significantly in both CAD patients and control
subjects carrying the R+R+ or R+R- ApoB genotype. In conclusion, EcoRI polymorphism in
ApoB gene does not play any significant role in the development of CAD in North Indian
population.
Keywords: Coronary Artery Disease, apoB gene, EcoRI polymorphism, North India
49
Biogenesis-V
Ankita Bhattacharya and Monika kesarwani
Department of Biotechnology, IILM-CET, Greater Noida,
Uttar Pradesh -201306, India
SCORPION VENOM: A WEAPON TO FIGHT CANCER
Scorpions are well known for their dangerous stings that can result in severe consequences for
human beings, including death. Neurotoxins present in their venoms are responsible for their
toxicity. Venom from various scorpion types such as blue scorpion, deathstalker scorpion have
chemotherapeutic potential. Diluted venom from blue scorpion, from Cuba, has been used to
fight cancer. A major anti-cancer action of scorpion venom is based on Chlorotoxin (CTX). This
peptide found in the venom blocks small-conductance chloride channels that are highly relevant
in some cancers for migration and invasion of the healthy cells. CTX can interact with membrane
protein in eg. Glioma cells, preventing transmembrane chloride fluxes while that doesn’t happen
for the normal cells because chloride ion channel activity is abundant in malignant gliomas and
absent in normal brain tissues. This selective activity in cancer cells suggests a potential
treatment for cancer. Besides brain cancer cells, CTX binds to wide variety of tumor cells
including prostate cancer, intestine cancer and sarcoma. Chlorotoxin can also bind to MMP-2
(Matrix metalloproteinase), a surface protein expressed in highly invasive tumor. When CTX
binds to MMP-2, both get drawn into the cancerous cells, reducing the expression of MMP-2.
Once inside cancerous cells, they also inhibit the process that allows cells to change shape,
which is required for the tumor cells to slip through the body and get to new location
(metastasis).
Keywords: venom, chlorotoxin, cancer, chemotherapeutic effect.
50
Biogenesis-V
Shyam Sunder*, Mohamed Shaffi* and J.S. Sandhu*
*FICCI Research and Analysis centre, Dwarka Delhi 110077
MICROBIOLOGICAL QUALITY ASSESSMENT OF WATER
AVAILABLE TO COMMON TRAVELLERS IN DELHI NCR
Common travellers take railway water supply at the platforms and may also purchase a tumbler
of chilled water sold on trolley at the road side, however, the safety and the microbiological
quality of the water supply is anybody’s guess. In view of the unknown quality of water supply it
was decided to test water samples from Railway Stations and Water Trolley near Railway
Stations. Samples were drawn from Railway Station Platforms (Sheetal Jal) and Water Trolleys
in a sterilized plastic bottle and taken to FICCI RAC Lab at Dwarka for analysis. The water
supply available to poor/common passenger was a mixed kind from railway stations where TPC
was nil to 1500 per ml, Coliform <2/MPN/100ml to >1600MPN/100ml, E. Coli nil/100ml to
>1600MPN/100ml, Yeast and Moulds were absent /250ml to present/250ml , Sulphite Reducing
Anaerobic Spore formers were absent/50ml to present/50ml. Other pathogens, V. Cholera,
V.Parahaemolyticus, Salmonella, Shigella, Pseudomonas, Staphylococcus aureus and Faecal
Enterococci were absent in 250ml. The railway water supply was safe in some cases but not safe
in other cases due to presence of E. Coli, Sulphite reducing anaerobic spore formers.The water
samples from Water Trolleys was found to be hazardous due to high TPC/ml 2000/ml to
0.12x106/ml, Coliform 300MPN/100ml to >1600MPN/100ml, E. Coli 8MPN/100ml to
>1600MPN/100ml, Sulphite Reducing anaerobic spore formers, Salmonella, V. Cholera,
Staphylococcus aureus were also present. The water available from Water Trolleys is hazardous
and unfit for consumption due sewage contamination and presence of pathogens in the water
charging source. The water from Water Trolleys is a contaminated supply system; it needs
monitoring and regulation by Food Safety Authority because it is posing a health hazard to
consumers.
Keywords: food safety, water analysis
51
Biogenesis-V
Mahima Srivastava , Homa tasnim, Priyanka roy
Department of Biotechnology, IILM-CET, Greater Noida,
Uttar Pradesh -201306, India
Email Address: [email protected]
3D PRINTED BLOOD VESSELS
3D printing is a process of creating cell patterns in a confined space using 3D technologies. A
Chinese biotechnological company announced that it recently has developed the world’s first 3D
blood vessel bioprinters which makes it possible to produce organs and 3D printed blood vessels
is implanted in 30 rhesus monkeys which successfully functioned as the monkey’s original
vessel without any rejection by its immune system because the monkey’s own stem cells were
used in Sichuan Revotek, known as Bio-ink. They printed a structure containing endothelial cells.
Researchers first create a 3D model of the biological structure on a computer. The computer then
transfers 2D snapshots of model to millions of microscope-sized mirror. The UV patterns are
shined onto a solution containing live cells and light sensitive polymers that solidify upon
exposure to UV light. The structure is rapidly printed one layer at a time in continuous fashion
creating a 3D solid polymer scaffold encapsulating live cells that will grow and become
biological tissue. It is truly a breakthrough in medical history. No longer patients in need of
transplants have to wait for suitable donors. The research on stem cell has been going on for a
long time now and now they have got their fruits of labor. Also the question comes to mind now
that they can make blood vessels and thereby organs they can produce legs, hands. This would be
a boon for people haste to survive on artificial limbs.Besides bioprinting, it enables
Decentralization; reduce costs, rapid prototyping,drug discovery.Well in India, it is not yet
developed because it comes with copyright issues, lots of limitations,produces a lot of water; it’s
still expensive for a majority of Indians to afford.
Keywords: cell pattern; biological structure; 3D solid polymer; Decentralization; reduce costs;
rapid prototyping; drug discovery; Bio-ink.
52
Biogenesis-V
Arpit Kumar,* Vijay Kumar* and J.S. Sandhu
*FICCI Research Analysis Centre Dwarka Delhi 001177
MICROBIAL QUALITY ASSESSMENT OF STREET FOOD AT
RAILWAY AND METRO STATIONS OF DELHI NCR
Street Food serves an important link in the socio economic niche of urbanites who are generally
travelling for long hours. The food available at railway stations and metro station fills in the need
of prepared food supply requirements of people on the go. The food generally picked up is
Burger, Sandwiches and Patties. The safety and hygiene of these foods is not known, therefore it
was decided to examine the microbiological quality of Burgers and Sandwiches. The average
count of TPC cfu/gm, coliform cfu/gm, yeast & mould cfu/gm were 110x106, 0.3x106, 0.2x106
respectively ss. E.coli was present in two cases only. Other pathogen like salmonella, B.cereus,
S.aureus, were absent indicating that the risk of getting a gastrointestinal disease was lesser from
the foods available at Metro & Railway station Better hygienic preparation efficient cold chains
for transparent storage and display (<80C) Can help to decrease the risk from these foods also
heating foods for three minutes to bring the temperature to 700C can further improve the quality
of street foods. The patties sample analyzed at less then 100 TPC cfu/gm, coliform, E.coli, Yeast
& mould, Salmonella, B.cereus and S.aureus were absent
Keywords: microbial quality assessment;
53
Biogenesis-V
Nainika Tanwar, Aishwarya Gupta and Sargam Verma
Department of Biotechnology, IILM-CET, Greater Noida,
Uttar Pradesh -201306, India
GLOBALIZATION OF HERBAL HEALTH- (A REVIEW)
Globalization of herbal health basically includes the large no of herbs present in our
surroundings and their use in the different biological activities. Present review emphasises on
herbs with anti-diabetic activity and anti HIV activity. Many herbs can elicit a decline in blood
glucose level in laboratory rodents render diabetic by alloxan or streptozotocin treatment or in
normal fasting animals. Natural products capable of inhibiting two of the HIV enzymes – HIV
proteases and HIV integrase, are reviewed in this article: flavones, terprnoids including
carnosolic acid, ursolic acid, margostin, gamma-margostin, a procyanidin dimer(procyanidin B1)
and two trimer( are catannins A1 and B1) are HIV proteases inhibitors. Extracts of plants native
to china, Indonesia, India, Japan and New Zealand have shown inhibitory effects towards HIV
protease. Compounds with multiple aromatic rings and aryl orthohydromylation such as
flavones, caffeic acid , phenyl ethyl ester,arcitingenin based compounds and Bis- catechols and
ribosome inactivating proteins are HIV integrase inhibitors.
Keywords: anti-diabetic, hyperlipidemic, HIV integrase, HIV protease, HIV reverse
transcriptase, carnosolic acid, urosolic acid,margostin,flavones.
54
Biogenesis-V
Anshuman Chandra1, Punit Kaur2, Nagendra Singh1, and Imteyaz Qamar1
1School of Biotechnology, Gautam Buddha University, Greater Noida,
Gautam Budh Nagar, U.P.201308, India
2Department of Biophysics, All India Institute of Medical Sciences, New Delhi
E-mail: [email protected]
DESIGNING OF POTENTIAL ANTAGONIST OF SRPK1 FOR
APPLICATION IN CANCER THERAPY
Splicing Factor 2/Alternative Splicing Factor (SF2/ASF) is the best characterized member of the
SR family of splicing factors which has been reported to be up-regulated in multiple cancers and
plays important roles in the establishment and maintenance of cell transformation. The SF2/ASF
is an oncoprotein which gets phosphorylated by Serine / arginine-rich protein kinase 1 (SRPK1),
which is a critical step for its activation and role in alternative splicing in cancer. In the present
study we have designed specific and potential inhibitors of SRPK1, which could be potential
therapeutic agents against cancer. More than a million of potential compounds (Zinc database)
were screened via computational methods using virtual screening workflow of Schrodinger suite,
targeting ASF2-SRPK1 interaction sites. The pharmacokinetic properties of these molecules
were analyzed by Oikprop. Top 400 compounds were selected and prioritized based on their
respective G/MmGbsa Score. The results showed that lead compounds have strong potential to
be developed as efficient inhibitors of SRPK1-SF2 complex. Further invitro/invivo studies would
help us to validate the potential inhibitors and their mechanism of inhibition of cancer states.
Keywords: antagonist of srpk1, cancer therapy
55
Biogenesis-V
Pooja choudhary and Ved Prakash
Department of Biotechnology, IILM-CET, Greater Noida,
Uttar Pradesh -201306, India
Email Id: [email protected]
BIOSURFACTANTS
Biosurfactant are organic compounds belonging to various classes including glycolipids, fatty
acids, phospholipids, neutral lipids, and lipopolysaccharides. These are produced on living
surface mostly microbial surface or excreted extracellularly. Microbes synthesis them during
growth on water immisible substrates, providing on alternative to chemically prepared convential
surfactant. Biosurfactant contain hydrophobic & hydrophilic moieties that reduces surface
tension & interfacial tension between individual molecule at the surface &interface A majority of
bio surfactant are produced by Bacteria.
Bio surfactants are biodegradable and can be produced from renewable and cheaper substrate.
Bio surfactant technology can be an effective and non-destructive method for bio remediation of
cadmium and lead contaminated soil.
Keywords: Biosurfactant, Microbes synthesis
56
Biogenesis-V
Akash Chaudhary, Ritvesh and Anubha Sharma
Amity Institute of Biotechnology, Amity University, Noida, UP, India
CHARACTERIZATION OF METAL TOLERANT BACTERIA ISOLATED
FROM HEAVY METAL POLLUTED GLASS INDUSTRY FOR ITS ROLE
IN BIOREMEDIATION
Heavy metal contamination of the environment is a serious concern because of their deleterious
effects on biological systems. To the best of our knowledge, this is the first investigation on
isolation of heavy metal resistant bacteria from contaminated sites of glass industrial area. The
study focused on isolation and characterization of Ni2+ resistant bacteria from these sites and
analysis of their Ni2+ accumulation potential. Out of 38 different bacterial isolates 3 bacteria
were able to tolerate up to 24 mM Ni2+ concentration. These bacterial strains were identified as
E. coli (AS17b), Escherichia coli (AS21) and Microbacterium sp. (AS33) by 16S rRNA (16S
ribosomal RNA) sequencing and their basic local alignment search tool search analysis. Growing
cell of E. coli and Microbacterium sp. revealed accumulation of 0.12, 0.08 and 0.06 mg, of Ni2+
/mg dry weight of cells, respectively, by 72 hr. Similarly resting cell of these strains showed
accumulation of 0.27, 0.11 and 0.08 mg of Ni2+ /mg dry weight of cells by 150 min, respectively.
These results reveal that strain E. coli (AS21) shows maximum accumulation efficiency for Ni2+
among different isolates studied under shaking as well as starving conditions. Hence, E. coli
(AS21) could serve as an efficient and promising bacterium for bioremediation of nickel
contaminated sites.
Keywords: heavy metals, glass industry, metal tolerant bacteria
57
Biogenesis-V
Priya Rai
Department of Biotechnology, IILM-CET, Greater Noida,
Uttar Pradesh -201306, India
E-mail:[email protected]
PROTEIN FOLDING INVOLVING MOLECULAR CHAPERONES
Protein Folding is a process in which a polypeptide folds into its characteristic, specific,stable,
functional, three-dimensional structure. The correct three-dimensional structure is essential to
protein function and is determined by the amino-acid sequence or primary structure (Anfinsen’s
dogma). The amino acid sequence of a polypeptide chains contains all the information required
to fold the chain into its native three-dimensional structure. Failure to fold into native structure
produces inactive proteins but in some instances misfolded proteins have modified or toxic
functionality. Several neurodegenerative and other diseases are believed to result from the
accumulation of amyloid fibrils formed by misfolded proteins. Protein folding requires molecular
chaperones which are proteins that facilitate the folding. It binds to incompletely folded or
unfolded proteins in order to assist their folding or prevent them from aggregation. Folding
begins to occur even during translation of the polypeptide chain. Amino acids interact to each
other to produce a well-defined three-dimensional structure, the folded protein called as native
state. However, the process also depends on the nature of solvent, the concentration of salts, the
temperature,and the presence of molecular chaperones.
Keywords: Anfinsen’s Dogma, misfolded, neurodegenerative, amyloid fibrils, chaperones.
58
Biogenesis-V
N N Tiwari1, R K Jain2 and A K Tiwari1
UPCSR Shahjahanpur, UP, Anand engineering college , Agra, UP
MOLECULAR CHARACTERIZATION OF PHYTOPLASMA
ASSOCIATED WITH CATHARANTHUS ROSEUS IN CENTRAL UTTAR
PRADESH
The typical phytoplasma symptoms of little leaf, witches’ broom and little leaf yellowing were
observed on Catharanthus roseus plants during the field survey conducted at central Uttar
Pradesh (Shahjahanpur, Bareilly and Barabanki) in year 2015–2016. To confirm the phytoplasma
etiology, total DNA were isolated from the infected as well as healthy. PCR and nested PCR
were performed with the universal primer pair P1/P6 and R16F2/R16R2 primers respectively.
Expected ~1.2 kb amplicon were observed in all the six symptomatic leaf samples collected from
Bareilly, Shahjahanpur and Barabanki (two from each location) which confirm the association of
phytoplasma with collected symptomatic leaf samples. Amplified products were eluted and
directly sequenced form both the direction and obtained sequences were used in BLASTn query.
Highest 99% sequence identity was recorded with the several isolates of Ca P asteris group of
phytoplasma. Phylogenetic analysis also confirms the BLASTn results. Further RFLP is in
progress. The C. roseus phytoplasma in the present study was identified as an isolate of 16Sr I
group Further study at sub group level identification is in progress.
Keywords: phytoplasma; Catharanthus roseus plants
59
Biogenesis-V
Ramsha Hashmi, Mohita Chugh and Neha Tiwari
Department of Biotechnology, IILM-CET, Greater Noida,
Uttar Pradesh -201306, India
Email Id: [email protected], [email protected]
CRISPR: THE FUTURE OF DNA EDITING
CRISPR-Cas System is a bio machinery which has evolved in prokaryotes like bacteria and
archaea to function as an adaptive immune system against a variety of environmental stressors
like infectious viruses and plasmids. The precision feature of this system is mediated by Cas
nuclease and small RNA guides which specify a cleavage site within the genome of the invader
organism. Cas 9 can be reprogrammed to create double stranded DNA breaks at a specific
location in the genome of a variety of organisms including human cells. The incorporation of
these short sequences from the genome of the invader into an exclusive region inside the hosts
genome is also mediated by this bio machinery. These exclusive regions in prokaryotes are
distinguished by Clustered Regularly Interspaced Short Palindromic Repeats that is abbreviated
as CRISPR. We will discuss about how this bio machinery is being used as a potent weapon by
understanding its mechanism of working with the help of a poster presentation to give a new
insight to our audience.
Keywords: Bio machinery, Archaea, Adaptive immune system, plasmids, Cas nuclease, RNA
guides.
60
Biogenesis-V
Prerit Gupta, Sugandha Katiyar, Dr. Mohammad Zeeshan Najim
Department of Biotechnology, Noida Institute of Engineering and Technology,
Greater Noida
[email protected], [email protected], [email protected]
WATER BLOBS: A SUBSTITUTE AND NOVEL APPROACH FOR
REMEDIATION OF WASTE GENERATED FROM POTABLE WATER
BOTTLES
Every year, India produce more than 21,899 tonnes of plastic waste from which 6000 tonnes of
plastic remain uncollected and littered. As a thought and rethink for remediation of plastic waste,
an edible and pliable container can be used in place of plastic, which is digestible and easy to
recycle. The Water Blobs are made from Sodium Alginate and Calcium Lactate, a potential
substitute of plastic container. The process of Reverse Spherification and reaction of Sodium
Alginate, which is a naturally occurring sea weed, reacts with Calcium Lactate to form a
gelatinous layer trapping water in it. This could really pave a way to reduce tonnes of non-
recycled, littered and uncollected plastic. This innovative approach for plastic can keep water
more hygienic, safe, and clean taking us a step toward sustainability. This sea weed is easily
available and can be grown in controlled laboratory environment, totally digestible by our body
and is eco-friendly too. These water blobs can be thought as water balloons with the edible outer
covering instead of water bottles or water packaging material. Water Blob can be stable for a
week without degradation in its gelatinous layer. These can be made easily and can be used to
study further for reducing plastic waste from earth. The consumption of non-renewable resources
for single-use bottles and the amount of waste generated is profoundly unsustainable. The aim of
water blob is to provide the convenience of plastic bottles while limiting the environmental
impact.
Keywords: Sodium Alginate, Calcium Lactate, Reverse Spherification
61
Biogenesis-V
Riya Marwah and Neha Srivastava
Department of Biotechnology, IILM-CET, Greater Noida,
Uttar Pradesh -201306, India
E mail address: [email protected]
MOLECULAR TOOLS FOR FORENSIC SCIENCE
Forensic Science uses many tool and techniques in order to solve the mystery behind criminal
cases. Molecular biology techniques have come up with most promising tool for identification of
prime suspect. It has evolved far beyond that which could have been predicted at the time DNA
identity testing was established. Indeed, we should now perhaps be referring it to "Forensic
Molecular Biology". Through which even a small changes in DNA sequences can be detected
which will help to differentiate individuals. Aside from DNAs established role in identifying the
'who' in crime investigations, developmental changes in medical and molecular biology now ripe
for appreciation to forensic challenges. The advent of Forensic DNA testing made contribution to
investigations. The whole investigation process includes collection of the physical evidences or
questioned samples from crime scene followed by its identification by physical, chemical and
molecular methodsin order to identify its origin, consequently identification of criminal. The
molecular application in forensic science involves the human identifying testing followed by non
phenotypic marker use, analysis of DNA firstly by RFLP technique and also by more efficient
revolutionary DNA fingerprinting technologies.
In this review, recent molecular techniques contributing to the advancement of Forensic Science
branch is discussed which are currently used by forensic scientist for investigation purpose. The
benefits, risk, precautions and success rate associated with each techniques are also a point of
concern which have also summarized in this review. It is apparent that these emerging
technologies will expand Forensic Science to allow inferences about 'when' a crime took place
and 'what' took place.
Keywords: Molecular biology, Forensic DNA, human identification testing, non-phenotypic
marker.
62
Biogenesis-V
Sagar Bashyal1, Shubham Rai1, Hridoy R. Bairagya2 and T.P.Singh2* 1Department of Biotechnology, IILM-AHL, College of Engineering and Technology
Greater Noida-201306 2Department of Biophysics, AIIMS-New Delhi -110029
STRUCTURAL AND FUNCTIONAL ROLE OF CONSERVED WATER MOLECULES
IN BOVINE LACTOPEROXIDASE ENZYME: A STUDY BY STRUCTURAL
BIOINFORMATICS METHOD
Lactoperoxidase (LPO) is a heme containing glycoprotein with a single chain of 595 amino acid
residues and a molecular mass of ≈68 kDa. The enzyme is found in milk and other exocrine
secretions such as saliva, tears and airways. LPO enzyme contains a covalently linked heme
prosthetic group which is a derivative of protoporphyrin IX in its catalytic center. It contributes
to the non-immune host defense system by oxidizing halide and pseudohalide ions to produce
potent antimicrobial agent. The mechanism of inhibition of LPO by several aromatic ligands has
been discovered by analysis of structures of the complexes of LPO with known inhibitors. Our
computational structural bioinformatics approach deciphers the existence of fifteen invariant
water molecules (W1-W15) and reveals the stereo chemical and electronic consequence of those
conserved water molecules in LPO enzyme. The conserved water mediated H bonding
interaction from distal region of Heme (W1 --- HIS109 ---W2 --- HIS266 --- W3 --- W4 --- W5 -
-- W6) is observed in all the LPO X-ray structures that may be responsible for proton transfer
mechanism whereas proximal HEME bound to HIS 351 (ASN437 --- TRP493 --- ASN497 ---
W7 --- W8 --- W9 --- W10 --- W11 --- ASP93) is also recognized by the surface residues of the
enzyme. Interestingly, the guanidine group of Arg348 anchor heme moiety to maintain the stereo
chemical architecture of its pyrrole ring at heme binding pocket through H bonding interactions
of P2 --- W12 --- Arg348 --- P1 and P2 --- W12 – W13--- W14 --- P1. In addition, both the
propionate groups of two pyrrole rings are also recognized the surface residues by conserved
water mediated or salt bridge mediated (P1 --- Arg440 --- Asp443 --- W15 --- Arg177, P2 ---
W12 --- W13 --- His 444 --- Asn341) H-bonds. The results from this computational investigation
could be of interest to experimental community to study and provide a testable hypothesis for
experimental validation of non-catalytic residues (His444 and Asn341).
Keywords: structural bioinformatics method; bovine lactoperoxidase enzyme
63
Biogenesis-V
Sadaf Raza
Department of Biotechnology, IILM-CET, Greater Noida,
Uttar Pradesh -201306, India
Email: [email protected]
APPLICATIONS OF CRISPR - CAS9
CRISPR (clustered regularly interspaced short palindromic repeats), is a system harnessed to
target specific stretches of genetic code and to edit DNA at precise locations. It allows
researchers to permanently modify genes in living cells and organisms and, in the future, may be
used to correct mutations and treat genetic disease. It is referred to as a revolutionary genome
editing technology that enables efficient and precise genomic modifications in a wide variety of
organisms and tissues. Utilizing a modified bacterial protein and a RNA that guides it to a
specific DNA sequence, the CRISPR system provides unprecedented control over genes in many
species, including humans. Cas9 is the first of several restriction nucleases that enable CRISPR
genome editing. CRISPR-Cas9 has a lot of potential as a tool for treating a range of medical
conditions that have a genetic component, including cancer, hepatitis B or even high cholesterol.
Many of the proposed applications involve editing the genomes of somatic cells but there has
been a lot of interest in and debate about the potential to edit germ line cells. The CRISPR-Cas9
system currently stands out as the fastest, cheapest and most reliable system for ‘editing’ genes.
Keywords: CRISPR, DNA sequence, cas-9
64
Biogenesis-V
Nisha Yadav,1 Naveen Kumar,2 Seema Sehrawat,2 ,B. Lochab1* 1Department of Chemistry and 2Life Sciences, School of Natural Sciences,
Shiv Nadar University, Gautam Buddha Nagar, Uttar Pradesh, 201314, India.
E-mail: [email protected]; [email protected]
SYNTHESIS OF SMART NANOVEHICLE AND ITS VARIOUS
BIOMEDICAL APPLICATIONS
In recent years, besides drug mediated cure of disease, gene therapy is explored as a more potent
therapeutic and adaptive strategy to treat various genetic disorders including cancer. The fate of a
successful gene therapy is majorly dictated its efficiency in transporting genetic material inside
the infected cell before its degradation by enzymes. The fate of such treatment demands efficient
vehicles for high transfection efficiency along with the benefits of its stability, biocompatibility
and non-toxic nature. Moreover, widely used drug based therapy involve the usage of high
concentration of drugs accounting the death of healthy cells along with infected cells
deteriorating the patient life. The designing of improved vector can eliminate the safety concerns
of gene therapy and demonstrated remarkable therapeutic benefits in inherited of nano-delivery
vehicles and their efficacy for drug/gene mediated therapy - as an advancement in sustainable-
therapeutic analysis
Functionalized nano-graphene/ graphene oxide and their nanocomposites have gained
tremendous attention in the area of biomedicine owing to their biocompatibility, ease of
functionalization along with the benefit of other modified properties based on the tethered
polymers/conjugated units.The larger surface area at single atom thickness of graphene supports
them to act as nano-cargo for non-viral gene transfer vector and drug loading platforms. I will
present the work in which targeted gene/drug delivery has been performed using biocompatible
and covalently functionalized graphene oxide for efficient breast cancer therapeutics.
Key words: Nano particles, biomedicine, gene therapy, graphene
65
Biogenesis-V
Shambhavi Shukla and Pushkar Saw
Department of Biotechnology, IILM-CET, Greater Noida,
Uttar Pradesh -201306, India
E-mail address: [email protected]; [email protected]
EBOLA VIRUS DISEASE AND MANAGEMENT
Ebola virus has become an explosive topic in recent years due to drastic increase in the number
of infected people. Ebola virus is transmitted among people as a result of close contact with
blood,organs or other body fluids of an infected person. It is the most feared pathogen worldwide
for both human and non human primates. The incubation period may last upto 7 days and the
symptoms are visible approximately after 21 days of exposure to this disease. The disease is
often mortal due to certain complications involved including haemorrhagic fever,marked
lymphocytopenia and thrombocytopenia,increased aminotransferase levels,dehydration,multiple
organ failure,fulminantshock,etc. There is no FDA approved vaccine or treatment available in
the market till the date. Since,prevention is better than cure and due to lack of proper cure of this
disease,certain preventive measures can be taken which include a good public health
infrastructure and certain awareness programmes organised both by the government and the
NGOs active in a country. Certain vaccines developed from various strains against this virus are
under considerable study a. Hence,this review covers various major aspects of Ebola Virus
Disease and steps taken in its management.
Keywords: ebola, symptoms, transmission, haemmorhagic fever, diagnosis,prevention
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Biogenesis-V
Swati Raina, Arpita Roy, ArusheTickoo, Navneeta Bharadvaja*
Plant Biotechnology Laboratory, Department Of Biotechnology,
Delhi Technological University, New Delhi-110042, India
*Correspondence: [email protected], [email protected]
PHARMACOLOGICAL REVIEW OF CENTELLAASIATICA: A
POTENTIAL DRUG
Centellaasiatica belongs to the family Umbelliferae commonly known as“gotu kola” and also
“Mandukaparni” is a perennial, herbaceous creeper with kidneyshaped leaves, found in India, Sri
Lanka, Madagascar, South Africa, Australia, China, and Japan. Centella prefers to grow in
shady, moist, or marshy areas. It is found in most tropical and subtropical countries growing in
swampy areas, including parts of India, Pakistan, Sri Lanka, Madagascar, and South Africa and
South pacific and Eastern Europe. In India it is found throughout moist places up to an altitude of
1800 m. The chemical constituents of this plant that make it a potential drug are saponinswhich
include asiaticosides, responsible for wound healing and vascular effects. Other components are
brahmoside and brahminoside, responsible for CNS and uterorelaxant actions, Centelloside and
its derivatives are found to be effective in the treatment of venous hypertension. In addition, the
total extract contains plant sterols, flavonoids, and other components such as tannins,
phytosterols, mucilages, resins, free amino acids, flavonoids, an alkaloid, fatty acids. The
pharmacological properties of this pant which acts as remedy for are CNS disorders like
epilepsy, schizophrenia and cognitive dysfunction. It also finds use in renal stones, leprosy and
skin diseases, anorexia and asthma additionally used in the management of diarrhoea, cholera,
measles, jaundice, leukorrhoea, haematemesis, hepatitis, urethritis, toothache, syphilis, smallpox,
neuralgia, rheumatism, toothache and varices; and as an antipyretic, analgesic and anti-
inflammatory anti-cancer, anti- depressant. A lot more properties of Centella are still being
researched and are under curtains.
Keywords: Centellaasiatica,
67
Biogenesis-V
Sheetal Gupta and Shalini Singh
Department of Biotechnology, IILM-CET, Greater Noida,
Uttar Pradesh -201306, India
E mail address: [email protected] , [email protected]
DIFFERENTIATING ABILITY OF STEM CELLS
Stem cells are undifferentiated cells from which the other cells originate.Stem cells have the
ability to divide indefinitely or differentiate into other cell types.The ability of stem cells to
differentiate varies.Some stem cells differentiate only into cells of certain tissues. These types of
stem cells have differentiating power which is known as pluripotent.eg.embryonic stem cells
originate as innermass cells within a blastocyst. These stem cell can become any tissue in the
body excluding a placenta. Some stem cells can differentiate into many cell types this
differentiating ability of stem cells is known as multipotent eg.a multipotent blood stem cells
,hematopoietic cells and this cell type can differentiate into several type of blood cells like
lymphocytes, monocytes etc. Another stem cells which has the capacity to differentiate into only
one cell type is unipotent cell egg.skin cells are one of the most abundant type of unipotent stem
cells. The stem cells which has the ability of differentiating single cell into an organism is known
as totipotency.eg spores and zygote
Keywords: undifferentiated; differentiated; pluripotent; multipotent,
68
Biogenesis-V
Arushe Tickoo, Arpita Roy, Swati Raina, Navneeta Bharadvaja*
Plant Biotechnology Laboratory, Department of Biotechnology,
Delhi Technological University, New Delhi-110042, India
Email ID: [email protected], [email protected]
A REVIEW ON PHARMACEUTICALLY IMPORTANT MEDICINAL
PLANT TRILLIUM GOVANIUM
Trillium govanium commonly known as “Naag Chatri/ Teen Patra” is an overexploited
endangered plant species. It belongs to the Trilliaceae family and distributed mainly in Asia
(Japan, China, India and Pakistan). It is distributed between 2300 – 4200m across Himalayan
region, district Kullu (Himachal Pradesh) being the major producer of wild Trillium species with
an yearly production of 8280 – 8520 Kg (approx.) in India. The plant is a small herb and its roots
are highly important due to the presence of compound called “Trillarin”. It also contains several
other bioactive compounds such as steroids, glycoside, flavonoid, terpenoids and saponins.
Trillarin on hydrolysis renders a corticosteroid, diosgenin mainly used as a sex hormone. T.
govanium rhizome is used as an analgesic, anti-inflamatory, antioxidant and anticancer drug. It
has found a great application against microbes and used as an antimicrobial and antifungal agent
against certain species. Trillium govanium, a lesser known medicinal plant in previous years that
gained popularity due to its folkloric uses, can be a promising candidate for therapeutic drug.
Due to its medicinal properties and extensive traditional use it is an overexploited plant. This
review provides an overall summary of phyto-constitutes and pharmacological activity of
Trillium govanium. The detailed pharmacological investigations are required for this plant so that
medicinal activities of this plant could further be exploited.
Keywords: Trillium govanium, Trillarin, phytocompounds, pharmacological activity.
69
Biogenesis-V
Shimona Chhetry, Rishika Sharma and Neha Nanda
Department of Biotechnology, IILM-CET, Greater Noida,
Uttar Pradesh -201306, India
E-mail address: [email protected]
ALGAE-FUELED BUILDINGS
There is always talk of futuristic building technologies, but few firms are able to break new
ground in some of the most promising directions. If there is to be a new ‘living architecture’
movement involving micro-climates and responsive materials, it proves itself on the forefront of
its exploration. Bio-Intelligent Quotient (BIQ) is the one of the world’s first advanced and
economical pilot project (situated in Hamburg, Germany.) among those kinds of futuristic
building technologies which implements bio-reactive façade (solar-leaf) in a residential building.
It is a five-storey algae powered structure. It has 200 square meter of algae filled bio-reactive
paneling and exemplifies a building integrated system absorbing Carbon-dioxide emissions by 6
tons per year, cultivating micro-algae to generate biomass and heat as a renewable resource. This
innovative system integrates additional functionalities like dynamic shading, thermal insulation,
noise abatement, highlighting the full potential of this technology. The building implemented
with algae, being used as “Bio-fuel source”. It draws all of the energy needed to generate
electricity and heat from renewable sources. In a developing countries like India, where there is
an abundant amount of renewable resources, optimum conditions for growth and development of
flora and funa as well as advanced infrastructure, economical pilot project like BIQ can come up
as promising technology to generate energy at the cost of ecological balance.
Keywords: living architecture; Bio-Intelligent Quotient; solar leaf; bio-fuel source; algae
biomass; bio-reactive façade
70
Biogenesis-V
Yatin Aggarwal and Garima Sharma
Department of Biotechnology, IILM-CET, Greater Noida,
Uttar Pradesh -201306, India
E mail address: [email protected]
FIGHTING CANCER WITH NANOTECHNOLOGY
Nanotechnology has the potential to increase the selectivity and potency of drugs while
minimizing the collateral toxicity to non-malignant cells. The application of nanotechnology in
medicine is often referred as Nano medicine which may revolutionize our approach to
healthcare. It combines basic sciences like biology and chemistry with engineering medicine and
also open numerous pathway to overcome a barriers posed by human body compared to
conventional approach. However, the diagnosis and treatment of cancer with nanotechnology is
still under development. Improvement of chemothereuptic delivery through enhanced solubility
and prolonged retention time and the improvement of some nano-carriers based drugs are already
present in market. Researchers have developed a method of delivering anti-cancer drugs to vital
organs like lungs and liver using a nanoparticle generator that can bypass a tumour cells ability
to develop drug resistance. Scientists have reached a new milestone in cancer therapy by
introducing cancer immunology in which body launches its own immune defence capable of
attacking on spreading tumours. The latest treatment is based on injecting the patient with a
porous silicon material that has been absorbed with an anti-cancer drug. In this review we
summarize a recent development and techniques used in cancer treatment through
nanotechnology and also emphasis on targeted drug delivery.
Keywords: Nanomedicine, Nanocarrier, Nanoparticle, Anti- Cancer drug, Targeted Drug
delivery.
71
Biogenesis-V
Yetnesh Dubey and Trisha saha
Department of Biotechnology, IILM-CET, Greater Noida,
Uttar Pradesh -201306, India
EFFECT OF CREATININE ON COGNITIVE PERFORMANCE
Creatine is a naturally occurring amino acid found in our body and it helps supply energy in the
cells. Its use is mainly popularized by athletes in sporting events as they used Creatine
monohydrate to improve their performance. Creatine can bind to phosphate to make
phosphocreatine, and this acts as a “buffer” to make ATP split. According to many researchers
ATP can be made 12 times faster using phosphate reserves from phosphocreatine than by using
standard oxidative phosphorylation and a 70 times faster than making ATP de novo, When we
think hard, brain levels of phosphocreatine can drop pretty acutely while ATP levels stay
constant. In short it improves brain efficiency. Creatine is found mostly in meat, fish and other
animal products and levels of muscle Creatine are known to be lower in vegetarians. A number
of studies in different countries have been done on vegetarians and non-vegetarians and effects
of Creatine on them. Creatine supplementation seems to have an effect on memory of the people
rather than their verbal skills. In many of the studies the reaction time of people was improved
with a better short term memory.
Keywords: creatinine, memory response, brain
72
Biogenesis-V
Shalvi Singh and Lubna Zeenat
Department of Biotechnology, IILM-CET, Greater Noida,
Uttar Pradesh -201306, India
SUPERBUGS AND MULTIDRUG RESISTANCE
SUPERBUGS or multi drug resistant (MDR) bacteria are the bacteria which have evolved during
the course of time to be resistant to a wide variety of antibiotics. Over usage of antibiotics and
incorrect use of antibiotics are the major cause of evolution of drug resistant bacteria. Due to
their ability to get mutated easily and change their genome, they continuously update their
defense mechanisms against various drugs which make the diseases caused by them, difficult to
treat. The powers of these superbugs have puzzled the researchers and worsened the conditions
of patients infected with these drug resistant bacteria. There are various ways or mechanisms
through which, a bacteria can become resistant to drugs. This review discusses our current
knowledge on these mechanisms, precautions and treatment to the diseases caused by the
superbugs.
Keywords: Multi drug resistance, antibiotics, superbugs
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Biogenesis-V
Teena Chandna
Department of Biotechnology, IILM-CET, Greater Noida,
Uttar Pradesh -201306, India
E-mail address: [email protected]
GENETIC ASPECTS OF ALZHEIMER’S DISEASE
Alzheimer’s disease is a chronic neurodegenerative disease, characterised by progressive
degeneration of neural cells due to development of amyloid plaques, neurofibrillary, or tau
tangles leading to loss of connections between neurons.
Alzheimer’s disease constitutes most cases of dementia (50-75%). The genetic factors leading to
the disease are investigated to be dominant autosomal mutations in amyloid precursor protein
gene (Chromosome: 21), presenilin 1 gene (Chromosome: 14) and presenilin 2 gene
(Chromosome: 1). Apart from these causative gene mutations, there are many risk factors like
apolioprotien E gene. This gene determines the age of onset of the disease, depending upon the
type of allele the subject is carrying. Hence there is a need to investigate the factors leading to
this disease, and furthermore finding its efficient preventive measures or even cure. Present
review discusses the various researches that deal with the genetic causes of Alzheimer’s disease
Keywords: Alzheimer’s disease, Genetic mutation, neurodegenerative disease
74
Biogenesis-V
Aditi Shrivastava
Department of Biotechnology, IILM-CET, Greater Noida,
Uttar Pradesh -201306, India
Email address:- [email protected]
VERTICAL FARMING
Vertical farming is the practice of producing food and medicine in vertically stacked layers,
vertically inclined surfaces or integrated in other structures.It is used to cultivate crop through
many different techniques like through artificial sunlight,this system also minimize the green
house effect, also in this different kind of water system and management system are followed in
the vertical farming. We use many resources to generate electricity or artificial sunlight like wind
mills,water pumping system, solar system and we also uses wind mills at the top of skyscrapers.
It requires several thing like water harvesting system, hydroponics techniques, fade glasses and
suitable architecture structure. In this crop can harvest at any reason in outer as well as interior
farming. By this there will be no crop loss occurred and also other benefits of interior farming
are stated as no bugs can infect crop and climatic changes are also not affect the crop. The
important thing in that there is less energy is utilized in this farming where LED are used instead
of sunlight for the growing of crop, better than under the sunlight. Basically this system reduces
labour cost.This is a very eco friendly method, we can cultivate any kind of crop at any duration
of time.multicrops can be cultivated in this process.in this the crop are protected under the
building so the plant and crop cannot be affected through rain.The water is used in this system
can be utilized again by recycling process. In this the plant and crop grow under vertical farming
are free from pesticides and it makes healthy food.
Keywords: agricultural techniques, greenhouse effect, management systems, ecofriendly;
pesticides.
75
Biogenesis-V
Gunjan Arya
Department of Biotechnology, IILM-CET, Greater Noida,
Uttar Pradesh -201306, India
STEM CELL THERAPY
They are unspecialized cells with an extraordinary ability to self-renew, capable of
differentiating into one or more specialized cell types playing a crucial role in homeostasis and
tissue repair. While the regeneration of a lost tissue is known to mankind for several years, it is
only in the recent past that research on regenerative medicine/dentistry has gained momentum
and eluded the dramatic yet scientific advancements in the field of molecular biology. The
growing understanding of biological concepts in the regeneration of oral/dental tissues coupled
with experiments on stem cells is likely to result in a paradigm shift in the therapeutic
armamentarium of dental and oral diseases culminating in an intense search for “biological
solutions to biological problems”. Stem cell therapy has become controversial following
developments such as the ability of scientists to isolate and culture embryonic stem cells, to
create stem cell using somatic cell nuclear transfer and their use of techniques to create induced
pluripotent stem cells. Initial evidence from pioneering studies has documented the likely
breakthrough that stem cells offer for various life-threatening diseases that have so far defeated
modern medical care.
Keywords: regeneration, stem cell, pluripotent cells
76
Biogenesis-V
Charu Sharma, Nishi Bhaduri and Vaishali Thapa
Department of Biotechnology, IILM-CET, Greater Noida,
Uttar Pradesh -201306, India
ANGIOGENESIS IN CANCEROUS CELLS
The following poster describes the mechanism of Angiogenesis in cancerous cells. Cancer cells
depend on larger supply of oxygen, nutrients and removal of waste products as compared to
normal cells and for this reason they form new blood vessels around them by secreting VEGF or
Vascular Endothelial Growth Factor. VEGF is a signaling protein that stimulates the formation
of new blood vessels around the cancerous cells and this process is known as Angiogenesis.
When a tumor cell encounters endothelial cell, VEGF secreted by the tumor cell binds to the
VEGF receptor present on endothelial cells signaling its nucleus to make genes required for
production of more endothelial cells. The Antiangiogenic therapy is one of the widely used
treatments which use angiogenic inhibitors that inhibit the vascular supply in the tumor cells.
The therapy has been used on 1445 patients uptil now, though it has shown non toxicity and long
term effects, it fails to give permanent cure and thus is still under progress. The poster also
discusses some of the recent under development researches for cancer such as Photodynamic
Therapy and use of engineered proteins such as Anthrax.
Keywords: Angiogenesis; Cancer cells
77
Biogenesis-V
Kumari Rhaeva
Department of Biotechnology, IILM-CET, Greater Noida, Uttar Pradesh -201306, India
Email address: [email protected]
RHEUMATOID ARITHRITIS AND ITS TREATMENT
Rhematoid arithritis (RA) is an autoimmune disease that causes chronic inflammation of the
joints and other areas of the joints. Autoimmune diseases are illnesses that occur when the
body’s tissues are mistakenly attacked by their own immune system. The symptoms and signs of
Rhematoid arithritis include joint pain, swollen joints, fever, limping, polyarithritis, loss of range
of motion, tender joints, loss of joint function, stiff joints, fatigue, joint redness, rheumatoid
nodules, anemia, joint warmth, joint deformity etc. The “rheumatoid factor” is an antibody that
can be found in the blood of 80% of people with rheumatoid arithritis. Rhematoid factor is
detected in a simple blood test. Possible risk factors for developing rheumatoid arithritis include
genetic background, smoking, silica inhalation, periodontal disease, and microbes in the
bowels(gut bacteria). There is no cure for RA. The treatment of rheumatoid arithritis involves a
combination of patient education, rest and exercise, joint protection, medications, and
occasionally surgery. Medication used in the treatment of rheumatoid arithritis include NSAIDs,
DMARDs, T-cell activation inhibitors, B-cell depleters, JAK inhibitors, immunosuppressants,
and steroids. The cause of RA is not known. Even though infectious agents such as viruses,
bacteria and fungi have long been suspected,none has been proven as the cause. It is also
believed that the tendency to develop rheumatoid arithritis may be genetically inherited.
Keywords: Rhematoid arithritis; autoimmune disease
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Biogenesis-V
Sapna Gandhi1 ,Dr. Keshav Malhotra ,Dr Jaideep Malhotra ,Dr. Narendra Malhotra ,Dr.
Neharika Malhotra, Shilpa Marwah, Chand Mohamad 1Department of Biotechnology, CET IILM AHL, Gr. Noida
ROLE OF DNA FRAGMENTATION IN PREVIOUS IMPLANTATION
FAILURES
Sperm DNA fragmentation has emerged as a potential to be one of the causative factor in male
infertility. DNA damage in human sperm is thought to correlate with impaired conception,
disrupted embryonic development, increased rate of miscarriages & morbidity in the offspring.
To evaluate the possible correlation between DNA fragmentation and Reproductive outcomes of
ART cycles, a cohort study of 95 infertile patients undergoing DNA fragmentation test was
designed. A literature search has shown that the most predictive cut off for pregnancy was < 25%
of fragmentation. DNA fragmentation test was done on patients with previous implantation
failures and 74% (71 patients) of the patients were found to have high DNA fragmentation of
>25%. Thirty seven of the total patients underwent an ICSI (Intracytoplasmic Sperm Injection)
cycle and restwere put on anti-oxidants therapy for follow up. In patients with more than baseline
fragmentation, 27% of the patients formed grade 2-3 embryos. 30% of the patients who
underwent ICSI cycle got pregnant with β-HCG value >100. 60% of these aborted or had a
biochemical pregnancy. High incidence of DNA fragmentation is found in patients with previous
implantation failures. Sperm DNA fragmentation greater than 25% could be associated with
higher probability of failure of ART treatment. Due to its strong correlation with several aspects
of ART procedures and further consequences for the offspring, sperm DNA fragmentation is a
parameter worth integrating in routine clinical practice.
Keywords: DNA Fragmentation Index, Recurrent Failure, Male Infertility
79
Biogenesis-V
Shanez Noorie Khan
Department of Biotechnology, IILM-CET, Greater Noida, Uttar Pradesh -201306, India
Email address: [email protected]
GREEN BIOTECHNOLOGY
Green biotechnology deals with the use of environment-friendly solutions as an alternative to
traditional agriculture, horticulture, and animal breeding processes .This is the application of
biological technique to plants with the aim of improving the nutritional quality, quantity and
production economics. An example is the designing of transgenic plants that are modified for
improved flavor, for increased resistance to pests and diseases, or for enhanced growth in
adverse weather conditions. Genetically enhanced crops are one tool that could contribute to a
more harmonious balance between food production and our surrounding environment. Besides
its application in agricultural sciences, green biotechnology can, and already do, contribute
positively to reducing CO2 emissions and anticipating the impact of climate change on food
scarcity. The aim is to provide information about the role green biotech currently plays, and can
play in future, in helping to combat climate change.
Keywords: Green biotechnology; animal breeding processes
80
Biogenesis-V
Vinita kumari,
Department of Biotechnology, IILM-CET, Greater Noida,
Uttar Pradesh -201306, India
E-mail address : [email protected]
GOLD NANORODS-KILLING CANCER IN THE HEAT OF MOVEMENT
Gold nanorods are one morphology of nanoscale object. Gold nanorods are formed by mixing
two chemical solution together citrate-capped gold nanospheres & bulk HAuCl4 growth solution.
Gold nanorods have a surface plasmon meaning they absorb or scatter light. Gold nanorods
produces heat when exposed to a near infrared laser with lipids,oleate and DOTAN. These gold
nanorods help in transporting cancer-killing genes TNF-related apoptosis-inducing
ligand(TRAIL) Into cells.Its surfsce Plasmon property make them more attractive for use in
catalysis & in biomedical application such as diagnostics,imaging and mostly used in cancer
therapy. This gold nanorods system provide a unique opportunity for site directed light inducible
transgene expression in mammabian cells by a near-infrared laser with minimal phototoxicity.
Keywords: Gold nanorods; phototoxicity; surface infrared laser; Plasmon.
81
Biogenesis-V
Nikita Sharma
Department of Biotechnology, IILM-CET, Greater Noida,
Uttar Pradesh -201306, India
Email address: [email protected]
PONCET DISEASE - 'TUBERCULOUS RHEUMATISM'
Tuberculosis arthritis accounts for approximately 1-3% of all cases of tuberculosis and
for approximately 10-19% of extrapulmonary cases. Tuberculous rheutamism is identified
as sterile reactive arthritis that can emerge during any stage of acute TB infection
involving large weight bearing joints, in particular the hips ,knees, and ankles, and
occasionally involve smaller nonweight bearing joints. Poncet's Disease- Tuberculous
Rheumatism is characterised by a clinical picture of polyarticular arthritis with active
visceral tuberculosis(TB) associated with pulmonary or extra pulmonary tuberculosis with
no evidence of mycobacteria infection inside the affected joints. The symptoms resolves
completely with anti-tuberculosis chemotherapy. The condition is different from tubercular
arthritis which is usually mono-articular and is caused by direct tubercular infection of
the joint. Poncet Disease remains a diagnosis of exclusion. It is a rare aseptic form of
arthritis observed in patients with active TB. Poncet Disease may manifest in a variable
pattern during the course of active tuberculous infection..Correct and timely diagnosis is
important as the usual treatment for inflammatory arthritis with immunosuppression might
lead to the dissemination of the infection.
Keywords: Tuberculosis; Poncet's Disease; Reactive Arthritis;inflammatory arthritis.
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Biogenesis-V
Shivangi Tripathi ,Shruti Singh Department of Biotechnology
IILM CET Greater Noida, Uttar Pradesh 201306
APPLICATION OF MONOCLONAL ANTIBODY IN THE TREATMENT
OF INFLAMMATORY CHRONIC DISEASES
Monoclonal antibodies (mAbs) are antibodies of a single antigen specificity produced by
identical immune cells, i.e., clones of a common germ cell. They offer unprecedented
opportunities to drug development because of their ability to target almost any cell surface or
secreted molecule with remarkable efficacy and safety. In this paper, the application of human
mAbs in the treatment of inflammatory diseases is reviewed with reference to Rheumatoid
arthritis (RA), Crohn's disease (CD), psoriasis, and other chronic inflammatory diseases.
Different cellular groups and inflammation mediators participate in the inflammatory process, all
of them susceptible of a therapeutic approach; they are so-called biological targets. Inhibition of
TNF and interleukina 1 (IL-1) has proven effective for the control of inflammation in diseases as
RA or CD. At present, we have two types of inhibitors of TNF, specific monoclonal antibodies
(infliximab, adalimumab) and cellular receptors (etanercept) and an IL-1 inhibitor (anakinra).
The use of TNF inhibitors has given rise to a substantial change in the treatment of RA and CD
because of its effectiveness. Together with this beneficial effect, an increase of infections (some
of them severe) has occurred, especially tuberculosis. Other side effects that can be considered
infrequent include demyelinization, heart failure, blood dyscrasias and lymphomas, which means
that a thorough knowledge of these drugs is necessary for their use. Other potential biological
drugs still in investigational phase are mentioned.
Keywords: Rheumatoid arthritis, psoriasis, interlurlekina
83
Biogenesis-V
Rishita De, Nitin Rai 1Department of Biotechnology, CET IILM AHL, Gr. Noida
GENETICALLY MODIFIED ORGANISM (GMO)
The concept of GMO generally refers to an organism whether a plant or an animal of a
microorganisms that has been produced using the modern biotechnology including recombinant
technology. A US definition of GMO refers to plant and animals containing genes transferred
from other species to produce certain characteristics, such as resistance to certain pests and
herbicides. GM crops available are Corn, soybean, cotton, papaya, canola, rice, tobacco, tomato,
potato, alfa-alfa, sugarcane. GM crops can be designed by identifying an organism with the
desired characteristics and transferring into host organism via vectors. Plant cells that take up the
new gene are grown into full size plants and are checked to make sure that they develop
normally and are safe. This GMO’s shows different advantage in our life for example the
targeted crops can be made herbicide resistant, Insects destruction are the target for the
modifications of the plant for higher productivity ,GMO have successfully developed plant with
resistance to virus ,Genetic engineering can be use to modify existing food to give them new and
enhance characteristics but this GMO perceives some problem also. In agriculture GMO poses a
range of environmental risk like transgene would transfer from GMO to wild relative whose
effects are still unknown ,the emergence of new form of resistance pests, or risk to human arises
if these product provokes an allergic or immune response .by above information we conclude
that with The development in biotechnology more and more GMO are introduced in commercial
markets but In my opinion, we can’t say good or bad without any scientific evidences as it has
both merits and demerits but at least it has shown a path with a light of hope for human beings.
Keywords: GMO, GM crops
84
Biogenesis-V
Shubhali Pandey[1], Faiz Hashmi[2] , Roma Chandra[3]
Department of Biotechnology, IILM CET, Greater Noida,
Uttar Pradesh -201306, India
DATA MINING: KNOWLEDGE DISCOVERY AND INTERACTIVE
DATA MINING IN BIOINFORMTIC AND IT’S APPLICATION
In this paper we provide an overview on interactive and integrative knowledge discovery and
data mining. The most important challenges, including the need to develop and apply novel
methods, algorithms and tools for the integration, fusion, pre-processing, mapping, analysis and
interpretation of complex biomedical data with the aim to identify testable hypotheses, and build
realistic models. The HCI-KDD approach, which is a synergistic combination of methodologies
and approaches of two areas, Human–Computer Interaction (HCI) and Knowledge Discovery &
Data Mining (KDD), offer ideal conditions towards solving these challenges: with the goal of
supporting human intelligence with machine intelligence. There is an urgent need for integrative
and interactive machine learning solutions, because no medical doctor or biomedical researcher
can keep pace today with the increasingly large and complex data sets – often called “Big Data”.
The application of data mining in the domain of bioinformatics is explained. It also highlights
some of the current challenges and opportunities of data mining in bioinformatics.
Keywords: Data Mining, HCI and KDD (Human-Computer Interaction, Knowledge Discovery
& Data Mining), Big Data, Interactive Knowledge discovery.
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Biogenesis-V
Anukriti Mishra
Department of Biotechnology
IILM CET Greater Noida, Uttar Pradesh 201306
GENETICALLY MODIFIED ORGANISM INTRODUCTION
Genitically modified crops are crops that have received a gene or a set of genes from another
species through genetic engineering. This process is achieved by horizontal gene transfer. Unlike
vertical gene transfer which is very much known gene transfer by almost everyone where the
traits are passed by the parents to the child, Horizontal gene transfer occurs when gene are
transferred from one organism to another.
The first recombinant drug approved by the FDA (food and drug administration) was humulin
(human insulin produced by bacterial), E.coil (bacteria )is used for such purposes as it replicates
every 20 minutes . Thus, the gene incorporated in the Bacteria is also replicated. This helps the
scientists to produce the drug with the help of the E Coil – A bacteria which doesn't require
much maintenance. Some Benefits of genetic engineering is increase in crop yields, reduced cost
for food or drug production, reduced need for pesticides , enhanced nutrient composition crop
yields, resistance to pests and disease , greater food security and medical benefits to the world's
growing population. Soyabean was genetically engineered for herbicide to lerance. Corn was
genetically engineered for resistance to insect pests, specifically the european corn borer, through
expression of the insecticidal protein cry1Ab from Bacillus thuringiensis. Plum became resistant
to plum pox virus conferred by insertion of a coat protein (cp) gene from the virus. In the United
States, 93% of cotton, 85% of cotton, 91% of soybeans grown were genetically engineered in
2013. Cows have been engineered to produce more protein and to resist diseases like Mastitis.
Genitically modified is also used as biological factories for producing rare or valuable materials.
One Example is 'Pharming' which uses crops and animals as bioreactors to produce vaccines or
drugs.
Keywords: GMO,
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Biogenesis-V
Nikita Sharma
Department of Biotechnology
IILM CET Greater Noida,
Uttar Pradesh 201306
STEM CELLS BASED THERAPY FOR TARGETTING SKIN TO BRAIN
CANCER
Investigations have a potential solution for how to kill tumor cells that have metastasized to the
brain. The research has developed cancer-killing viruses that can deliver stem cells via the
carotid artery, and applied them to metastatic tumors in the brain of clinically relevant mouse
models. The research first developed different BRAF wild type and mutant mouse models that
more closely mimic what is seen in patients. Injected cells allow to enter the brain and are
labelled with bioluminescent and fluorescent markers to enable tracking. A population of bone
marrow derived mesenchymal stem cells loaded with oncolytic herpes simplex virus (oHSV),
which specifically kills dividing cancer cells while sparing normal cells. It led to significantly
slower tumor growth and increased survival. PD-L1 immune checkpoint blockade significantly
improved the therapeutic efficacy of stem cell based oncolytic virotherapy in melanoma brain
metastasis. They have direct implications for designing clinical trials using oncolytic viruses for
metastatic tumors in the brain.
Keywords: Stem cells based therapy
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Biogenesis-V
A GLIMPSE OF DEPARTMENT OF BIOTECHNOLOGY
Dr. Pallavi Singh Ms. Roma Chandra
Ms. Garima Gupta
Dr. Sanjay Awasthi Dr. Meenu Singh
Dr. Charu Agarwal Dr. Dhiresh Pathak Dr. Avijit Guha
Dr. Avinash Singh Dr. Arpita Mishra Ms. Neha Srivastava Mr. Vikas Kumar
Ms. Neha Tiwari Ms. Charu Sharma
Co
nven
or
Organ
izing Secre
tary
Treasurer
