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Archiving of OCS Invertebrate Specimens and Genomic Tissue Samples NMNH, Department of Invertebrate Zoology August 2017 Gulf of Mexico Information Transfer Meeting (ITM)

Archiving of OCS Invertebrate Specimens and Genomic Tissue

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Page 1: Archiving of OCS Invertebrate Specimens and Genomic Tissue

Archiving of OCS Invertebrate Specimens and Genomic Tissue Samples

NMNH, Department of Invertebrate ZoologyAugust 2017 Gulf of Mexico Information Transfer Meeting (ITM)

Page 2: Archiving of OCS Invertebrate Specimens and Genomic Tissue

National Museum of Natural History, Smithsonian InstitutionDepartment of Invertebrate Zoology

(http://invertebrates.si.edu)

Dr. Jon Norenburg, Chair ([email protected])

William Moser, Acting Collection Manager ([email protected])

Page 3: Archiving of OCS Invertebrate Specimens and Genomic Tissue

Department of Invertebrate Zoology Collections

50 Million Specimens129,000 + unique taxa

representing 60 Phyla in 4 Kingdoms

Types: primary types of 40,000 + species

Collection Physical SizeMSC Pod 5: 18.1 linear miles of shelving and 95 steel tanksMSC Pod 2: 250 cases with 2,500 drawers       NHB: 1,700 cases with 14,000 drawers        2.3 acres of dry spm storage

And approximately 500,000 glass slides

Page 4: Archiving of OCS Invertebrate Specimens and Genomic Tissue

• In 1846, Congress passed legislation that laid the foundation for a partnership between the US National Museum (USNM) and the various Federal organizations that generated collections related to their mission. In this legislation, the Smithsonian was identified as the repository for natural history specimens belonging to the United States. 

• In legislation passed in 1879, Congress clarified and expanded the role of the US National Museum by designating the USNM as the final repository for collections made on behalf of the US Government ‐ when those collections were no longer needed for investigations in progress. 

• The intent of the legislation and the purpose of the resulting partnerships between the USNM and the various government organizations was to ensure the long‐term care of these collections and their accessibility for the benefit of the American people.

Page 5: Archiving of OCS Invertebrate Specimens and Genomic Tissue

• Over 35 Year History of Collaboration Between the Bureau of Ocean Energy Management’s Collection Archiving Project and the Smithsonian Institution, National Museum of Natural History, Department of Invertebrate Zoology

• NMNH’s Department of Invertebrate Zoology provides professional collection management services for the long‐term curation of marine invertebrate specimens taken during the environmental baseline surveys of various oil and gas lease sites on the US East and Gulf Coasts, and the US West and Alaskan Coasts

Page 6: Archiving of OCS Invertebrate Specimens and Genomic Tissue

Over 280,000 lots cataloged

Unique taxa in BOEM collection – 8,359Annelida, Crustacea, Cnidaria,Echinoderms, Mollusca, Porifera

Page 7: Archiving of OCS Invertebrate Specimens and Genomic Tissue

• Types – 1,518 lots; 357 new species• Searchable online catalog; results 

downloaded as spreadsheet or interactive map; linked to GeoESPIS

• Spms available for loan, scientific visit• Support for hazmat shipments for PIs 

(donation of material)

BOEM Collections at NMNH, Invert Zoology

Page 8: Archiving of OCS Invertebrate Specimens and Genomic Tissue

BOEM Maps: A geospatial tour of 40 years of collection

http://invertebrates.si.edu/boem/maps.html

Page 9: Archiving of OCS Invertebrate Specimens and Genomic Tissue

Isotopic Analysis: Sponge Metabolism Reproduction and Development of Cnidaria

Squid Life History Strategies: Statolith StudyGalatheoid Crab Morphology, Life History, and Parasites

Squid: eye size correlation to water depth

• The scientific value of BOEM voucher specimens extends well beyond the end of the ESP study and the final reports 

• As well as museum specimens for morphological, molecular, and population investigations 

Page 10: Archiving of OCS Invertebrate Specimens and Genomic Tissue

• In 2017, expansion of partnership to include barcoding of vouchers and collection of genomic tissue samples (fresh‐frozen, 95% EtOH, or DMSO)

Invertebrate Zoology Collection Processing Procedures (BOEM Project)http://invertebrates.si.edu/boem/presentations.htm

(See Chapter 5, Tissue Sampling and Preparing Molecular and DNA samples)

Page 11: Archiving of OCS Invertebrate Specimens and Genomic Tissue

Sampling BOEM collectionsat NMNH

Page 12: Archiving of OCS Invertebrate Specimens and Genomic Tissue

• Can provide labeled cryovials andmatrix plates

Page 13: Archiving of OCS Invertebrate Specimens and Genomic Tissue

NMNH’s Biorepository [20 liquid nitrogen freezers (‐190°C & ‐150°C) and 58 mechanical freezers (‐80°C & ‐30°C)]

Page 14: Archiving of OCS Invertebrate Specimens and Genomic Tissue

Family

444567 633

928

Families not represented in GGBN (567)

Not in GenBank(633)

SI NMNH BOEM Programs

(928)

DNA Barcoding 

Page 15: Archiving of OCS Invertebrate Specimens and Genomic Tissue

The following families have less than 5 genera to target:Abylidae 3Acanthogorgiidae 1Actinernidae 1Actiniidae 4Actinoscyphiidae 1Actinostolidae 1Aequoreidae 2Agalmatidae 1Agariciidae 3Aiptasiidae 1Aiptasiomorphidae 1Alatinidae 1Alcyoniidae 3Aliciidae 1Anthoptilidae 1Anthothelidae 2Antipathidae 4Astrocoeniidae 1Atollidae 1Atorellidae 1Boloceroididae 1Bougainvilliidae 3Campanulariidae 2Campanulinidae 3Cerianthidae 2Chiropsalmidae 1Chrysogorgiidae 2Cladopathidae 1Coralliidae 1Cuninidae 1Cyaneidae 1Edwardsiidae 1Eirenidae 1Ellisellidae 4Epizoanthidae 1Eudendriidae 1Flabellidae 3Fungiacyathidae 1Funiculinidae 1Geryoniidae 1Guyniidae 4Halcuriidae 1Haleciidae 1Halicreatidae 1Halipteridae 1Haloclavidae 1Halopterididae 2Hebellidae 1Hippopodiidae 2Hydractiniidae 3Isophelliidae 1Keroeididae 1Leiopathidae 1Lithotelestidae 1Lovenellidae 2Meandrinidae 1Merulinidae 1Milleporidae 1Minyadidae 1Mussidae 3Myriopathidae 3Nausithoidae 1Nidaliidae 3Oceaniidae 2Oculinidae 2Ombellulidae 1Pandeidae 1Paragorgiidae 2Parazoanthidae 1Pelagiidae 1Pennariidae 1Periphyllidae 1Physaliidae 1Plumulariidae 2Pocilloporidae 1Poritidae 1Porpitidae 2Prayidae 1Protoptilidae 2Renillidae 1Rhizangiidae 1Rhizostomatidae 1Rhopalonematidae 2Rosalindidae 1Sagartiidae 2Schizopathidae 2Siderastreidae 1Spongiodermidae 2Stylopathidae 1Syntheciidae 1Tamoyidae 1Tubulariidae 2Turbinoliidae 2Ulmaridae 1Umbellulidae 1Virgulariidae 3Zancleidae 1Zoanthidae 3(blank) 1Grand Total 155

55

24

6

7

76

6

9

6

5

17

56

Gap Analysis – Target CnidariaGulf of Mexico Fauna

Aglaopheniidae

Aphanipathidae

Caryophylliidae

Clavulariidae

Dendrophylliidae

Diphyidae

Faviidae

Gorgoniidae

Hormathiidae

Isididae

Lafoeidae

Plexauridae

Primnoidae

Sertulariidae

Target YesPhylum Cnidaria

Row Labels Count of GeneraAglaopheniidae 5Aphanipathidae 5Caryophylliidae 24Clavulariidae 6Dendrophylliidae 7Diphyidae 7Faviidae 6Gorgoniidae 6Hormathiidae 9Isididae 6Lafoeidae 5Plexauridae 17Primnoidae 5Sertulariidae 6Grand Total 114

Gap Analyses for the Phyla: Annelida, Bryozoa, Cnidaria, Arthropoda (Crustacea),Echinodermata, Mollusca, Porifera, and Misc. Phyla 

Page 16: Archiving of OCS Invertebrate Specimens and Genomic Tissue

9

68

37

5

114

187

233

1

Gap Analysis – Target Taxa in NMNH‐IZ collections (BOEM)(high priority: collected in last 10 years, dry, or in 95% EtOH)

Gulf of Mexico Region

Annelida

Arthropoda

Bryozoa

Chordata

Cnidaria

Ctenophora

Echinodermata

Mollusca

Sipuncula

Target Yes

Row Labels Count of GenusAnnelida 9Arthropoda 68Bryozoa 37Chordata 5Cnidaria 114Ctenophora 1Echinodermata 87Mollusca 233Sipuncula 1Grand Total 555

Page 17: Archiving of OCS Invertebrate Specimens and Genomic Tissue

Collections Management Policies and Procedures: Department of Invertebrate Zoology website (http://invertebrates.si.edu)

Loans (http://invertebrates.si.edu/loansPolicy.htm), Loan request form Scientific Visits (http://invertebrates.si.edu/visits.htm), Registration formDonating material (http://invertebrates.si.edu/donation.htm), Deed of Gift

Shipping Address (can assist with hazmat shipments):ATTN: William MoserDepartment of Invertebrate ZoologySmithsonian Museum Support Center4210 Silver Hill RdSuitland MD 20746Phone: 301‐238‐1761; e‐mail: [email protected]

Invertebrate Zoology Collection Processing Procedures (BOEM Project)http://invertebrates.si.edu/boem/presentations.htm

(See Chapter 5, Tissue Sampling and Preparing Molecular and DNA samples)

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