Upload
oleg-tirsina
View
35
Download
0
Tags:
Embed Size (px)
DESCRIPTION
bacterial disease of nuts and stone fruits
Citation preview
COST 873
Stone FruitNut Health
Central Science Laboratory, York, UK
Scientific Training Mission
Bacterial Diseases of Nuts and Stone Fruits
Marco Scortichini
C.R.A.- Centro di Ricerca per la Frutticoltura, Roma, Italy
3-6 March, 2008
A short survey of the global market of Nut and Stone Fruits
Traditional producers: Turkey, Italy, U.S.A., Spain, France
NUTS
Hazelnut
Emerging countries: Iran, China, Azeirbaijan, Chile
Walnut
Traditional producers: China, U.S.A., Turkey, France,
Italy, Spain
Emerging countries: India, Iran, Chile
Almond
Traditional producers: U.S.A., Spain, Italy, Greece, Turkey
Emerging countries: India, China
Pistachio
Traditional producers: Iran, U.S.A., Turkey, Syria
STONE FRUITS
Peach
Traditional countries: China, U.S.A., Mediterranean
Europe
Emerging countries: northern Africa (very early ripening
cultivars)
Apricot
Traditional countries: Europe, U.S.A., Eastern Asia
Emerging countries: Pakistan
Plum
Traditional producers: China, U.S.A., Serbia, Germany, Romania
Emerging countries: Chile
Cherry
Traditional producers: U.S.A., Turkey, Germany, Italy, Russia
Emerging countries: Iran
WALNUTXanthomonas arboricola pv. juglandis (Walnut bacterial blight)
- Worldwide distributed
- Limiting factor
- Difficult to control
- Different lineages of the pathogen (vertical oozing canker)
Brenneria nigrifluens (Walnut shallow bark canker)
- Worldwide distributed (suspected)
- Increasing importance
- Difficult to control
Brenneria rubrifaciens (Walnut deep bark canker)
- Apparently limited to California, cv. Hartley
Xanthomonas arboricola pv. juglandis
Main features:
- Isolation: easy-to-perform (YDC, GYCA, BS, Tween)
- Pathogenicity tests: need humidity
- Identification: still based on classical techniques
- Characterization: relevant genetic variability (rep-PCR,
AFLP) >>> geographic and/or plant organ structure ??
- Chemical control: copper spray treatments (not always
effective) >>> new compounds
- Occurrence of copper-resistant strains
- Is Xaj pollen-transmitted ?? (just one report)
- Brown apical necrosis (BAN): which role for Xaj ??
Walnut (Juglans regia L.) in Europe and Eastern Asia: adapted to local
environments
Isoenzimes + UPGMA
AFLP + Multiple canonical analysis
Brenneria nigrifluens
Main features:
- Isolation: not easy (typical symptoms but not isolates on plates)
- Isolation media: NSA, Eosin Blue Agar Medium (Difco)
- Pathogenicity tests: need time to reproduce the symptoms
- Identification: classical (urease test: doubtful) + molecular (rep-PCR)
- Molecular diagnosis: improved but to improve (to verify on
naturally infected samples)
A) 350 bp band, rep-PCR/REP
B) 250 bp, minisatellite M13 phage
- Brenneria: associated with trees >>> pathogenic on stressed plants ??
- Fungi play a role(s) in causing the symptoms ??
- Control: is possible ??
Brenneria rubrifaciens
Very limited knowledge: is present in Europe ??
Isolation: YDC (pink colour of the agar, not colonies)
HAZELNUT
Xanthomonas arboricola pv. corylina (Bacterial blight of Hazelnut)
- EPPO Quarantine pest (A2 list)
- Worldwide distributed
- Dangerous on young trees
Pseudomonas avellanae (Bacterial canker and decline of hazelnut)
- Very aggressive in northern Greece and central Italy
- Dangerous on acidic soils
Pseudomonas syringae pv. coryli (Bacterial twig dieback of Hazelnut)
- Known only in Italy and Germany
- Damages to twigs and trunk
Xanthomonas arboricola pv. corylina
Main features:
- Isolation: quite effective on GYCA, YDC (no selective media)
- Pathogeniciy tests: need humidity
- Characterization: to improve (limited number of international strains)
- Diagnosis: still based on classical techniques
- Cycle of disease: still largely unknown (role(s) of cankers, fallen
leaves, pollen)
- Control: to improve
Pseudomonas avellanae
Main features:
- Isolation: easy on NSA, (no selective media), difficult to maintain
- Pathogenicity tests: to perform in early autumn
- Characterization: relevant information (rep-PCR, ITS, MLST)
- Diagnosis: 16S, hrpW (effective, to improve on plant material)
- Control: copper and S.A.R.-related compound (Bion)
- Effective penetration into the tree via leaf scars
- Two different lineages of the pathogen (Italy and Greece)
- Parallel origin of the two lineages in acidic soils ??
- Is P. avellanae present in other countries ?: Slovenija, Croatia ??
MLST Genetic distance + Mantel test
Pseudomonas syringae pv. coryli
- The real distribution is unknwon
ALMOND
Xanthomonas arboricola pv. pruni (Bacterial spot of Almond)
- EPPO Quarantine pest (A2 list)
- Emerging in mediterranean Europe
- Present in India, Australia, New Zealand, U.S.A.
Pseudomonas amygdali (Bacterial canker of Almond)
- Reported only in Greece (Crete) and Turkey
Xylella fastidiosa (Almond leaf scorch)
- EPPO Quarantine pest (A1 list)
- A threat in California
Agrobacterium tumefaciens (Crown gall of Almond)
- To be checked in the nurseries (rootstocks)
Xanthomonas arboricola pv. pruni
Very limited knowledge about this pathogen on Almond
(only reports on the occurrence)
Pseudomonas amygdali
Xylella fastidiosa
Agrobacterium (Rhizobium) tumefaciens
Prunus webbii: resistant to artificial inoculation
PISTACHIO
Xanthomonas translucens pv. poae ?? (Pistachio dieback)
- Recently reported in Australia
- Oozing of resin, xylem staining in 2-3-years old twigs, decline
- Kochs postulate only partially fulfilled
PEACHXanthomonas arboricola pv. pruni (Bacterial spot of Peach)
- EPPO Quarantine pest (A2 list)
- Increasing importance in Europe
- Spreading mainly through latently infected propagative material
Pseudomonas syringae pv. persicae (Bacterial dieback of Peach)
- EPPO Quarantine pest (A2 list)
- Still limited to France (eradicated ?), England (?) and New Zealand (?)
Pseudomonas syringae pv. syringae (Bacterial canker and fruit scab of Peach)
- It can cause severe damage to fruits
Xylella fastidiosa (Phony Peach)
- EPPO Quarantine pest (A1 list)
- A future threat ?? (global warming + vectors spreading)
Agrobacterium tumefaciens (Peach Crown Gall)
- A problem for the rootstocks
Xanthomonas arboricola pv. pruni
Main features:
- Isolation: sometimes difficult (GYCA, YDC, NA)
- Pathogenicity tests: requires humidity and high temperatures (25-28C)
- Identification: combination of classical and molecular techniques
- Characterization: good results (rep-PCR, AFLP) at strain level
- Apparently restricted genetic variability
- Molecular diagnosis: to improve (sensitivity, specificity, vegetative samples)
A) 934 bp: RAPD specific fragment
- Control: difficult (copper phytotoxicity) >>> other compounds
- Biological control: how much is effective ??
- Statistical models for predicting the epidemics
- Role(s) of cankers (when present) in the cycle of disease
Restricted genetic variability of the host plant
AFLPfAFLP
Pseudomonas syringae pv. persicae
Main features:
- Reported only during the 70s
- Are still there field symptoms of the disease ?
- Not fluorescent on KB medium >>> CSGA
- Ice nucleation-active bacterium
- Predisposing factors: very cold winter, acidic soils, high nitrogen
very heavy soils
Pseudomonas syringae pv. persicae
RFLP + NJ
France
New
Zealand
UK
Pseudomonas syringae pv. syringae
P. syringae pv. syringae
P. syringae pv. persicae
Main features:
- It occurs occasionally but can be very severe
- It occurs early in the season but symptoms can overlap with those incited by
X. arboricola pv. pruni
- Little knowledge on its cycle of disease
- Control: difficult
Xylella fastidiosa
Main features:
- EPPO Quarantine pest (A1 list)
- Very aggressive on Grapevine and Citrus spp.
- There are lineages of the pathogen structured on host plant basis
>>>> subsp. ?, pathovars ?
- Isolation: difficult (long time)
- Pathogenicity tests: difficult (long time)
- Diagnosis: excellent for Grapevine (DAS-ELISA)
- Molecular diagnosis: to validate
A) Grapevine: 733 bp, 1,0 kb-EcoRI fragment
B) Citrus: 500 bp, RAPD specific fragment
- Are there vector(s) in Europe ??
- Could be the pathogen associated with other (European) weeds
and insects ??
Agrobacterium (Rhizobium) tumefaciens
- A problem in the nursery (GF 305, 677: very sensitive
- Effectiveness of K84 ?
APRICOT
Xanthomonas arboricola pv. pruni (Bacterial spot of Apricot)
- EPPO Quarantine pest (A2 list)
- Emerging pathogen on this crop (spreading in Europe)
Pseudomonas syringae pv. syringae (Bacterial canker of Apricot)
- It can cause severe damages and the death of the tree
- Some cultivar (i.e. Aurora) are very susceptible
Pseudomonas viridiflava (Bacterial canker of Apricot)
- It can be associated with P. syringae
Agrobacterium (Rhizobium) tumefaciens (Apricot Crown Gall)
- Problem in nursery
Xanthomonas arboricola pv. pruni
Pseudomonas syringae pv. syringae
Main features:
- It is an emerging disease mainly in temperate, non Mediterranean areas
- High susceptibility of the cultivar, spring frost and light soils are very
important predisposing factors
- Different lineages of the pathogen >>> new pathovar ?
- Control: difficult (as for any fruit tree species)
JAPANESE PLUM
Xanthomonas arboricola pv. pruni (Bacterial spot of Japanese plum)
- EPPO Quarantine pest (A2 list)
- It can be a limiting factor for the cultivation
- Very susceptible cultivars
- Emerging disease
Xylella fastidiosa (Plum Leaf Scald)
- EPPO Quarantine pest (A1 list)
- Very dangerous in the U.S.A.
Agrobacterium (Rhizobium) tumefaciens (Crown Gall of Plum)
- It is a problem in the nursery
Xanthomonas arboricola pv. pruni
Xylella fastidiosa
Agrobacterium (Rhizobium) tumefaciens
SWEET and SOUR CHERRY
Pseudomonas syringae pv. morsprunorum (Bacterial canker of Cherry)
- Endemic in many areas of cultivation
- Increasing in importance
Pseudomonas syringae pv. syringae (Bacterial canker of Cherry)
- Associated with PSM ??
Pseudomonas syringae pv. avii (Bacterial canker of Cherry)
- Associated with wild Prunus avium ??
Pseudomonas syringae complex
Cycle of disease of Pseudomonads
Main features:
- A Pseudomonas syringae complex
- Increasing importance for timber production and nursery
- Some lineages are associated with organs (i.e. buds, leaves, twigs) ??
- Rep-PCR can discriminate PSM race 1 from PSM race 2
- Diagnosis: difficult to find out a specific primer
- Which is the role of spring frost ??
MANY THANKS FOR YOUR KIND ATTENTION !!!
For further pictures:
www.atlasplantpathogenicbacteria.it