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Biacore™ t200 for high sensitivity, label-free interaction analysis

Amersham™ ecl™ Prime Western blotting reagent

Biacore T200 is a versatile, label-free system for detailed studies of biomolecular interactions. The system delivers high quality kinetic, affinity, concentration, specificity, and selectivity data in real-time with exceptional sensitivity. Interactions characterized by on- and off-rates at the extremities of the kinetic scale can be analyzed with greater precision and confidence than ever before.

The system is a highly flexible, general purpose system that covers the broadest range of assay types, and also offers a number of features that are of particular benefit to specific application areas. Typical applications of Biacore T200 are characterization of biomolecular interactions in life science research, low molecular weight and biotherapeutic drug discovery, immunogenecity testing in preclinical and clinical drug development, and vaccine development.

Biacore T200 system provides:

> High quality kinetics from the fastest on-rates to the slowest off-rates

> Analysis of interactions involving the smallest low molecular weight (LMW) compounds

> Automated and robust system - processes up to 384 samples in unattended runs

> Flexible software with high-level user guidance - for fast assay development, analysis, and evaluation

> Guided workflows with built-in data quality assessments and tools - get to final results faster

> Seamless transition into GxP-regulated environments using Biacore T200 GxP Package

Amersham ECL Prime is a sensitive, stable, and precisely quantitative Western blotting reagent that works across a wide dynamic range of protein levels, and allows conservative use of expensive antibody reagents.

Amersham ECL Prime offers:

> High signal intensity and sensitivity, which allows use of highly diluted primary and secondary antibodies

> Stable signal, which allows multiple exposures and large experimental series

> Compatibility with CCD-based imagers such as ImageQuant™ LAS 4000

> Compatibility with most commercially available Western blotting reagents such as molecular weight markers, membranes, and blocking reagents

> Improved performance compared with Amersham ECL Plus

The improved performance of Amersham ECL Prime compared with other chemiluminescent reagents is due to the presence of an enhancer in the reagent, increasing enzyme turnover, and thereby significantly increasing both signal intensity and duration. The light signal is further intensified by the addition of a catalyst.

ordering informationProduct code number

Biacore T200 Processing Unit 28-9750-01

Biacore T200 Upgrade Kit 28-9779-63

Biacore T200 GxP Upgrade Kit 28-9779-64

Biacore T200 GxP Package 28-9779-54

ordering informationProduct code number

Amersham ECL Prime, for 1000 cm2 membrane RPN2232

For more information, visit www.gelifesciences.com/biacore

For more information, visit www.gelifesciences.com/eclprime

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?Membrane Protein Purification Kit Membrane Protein Purification Kit is designed for efficient detergent screening of histidine-tagged membrane proteins. Small aliquots of cell membranes are solubilized in different detergents, followed by rapid purification using His Mag Sepharose Ni magnetic beads. Reliable recovery of captured protein is achieved using a magnet.

The purification step is performed directly after solubilization using the same detergent. Analysis and evaluation can be performed by a number of methods such as Western blotting, gel filtration, or light scattering.

Membrane Protein Purification Kit offers the following benefits:

> Easy solubilization and purification of membrane proteins

> Rapid detergent screening in a convenient magnetic bead format

> Simultaneous evaluation of solubilization and purification efficiency

> Ready-to-use detergent solutions

> Easy-to-follow protocol

Membrane Protein Purification Kit contains seven detergents (purity ≥ 99%), His Mag Sepharose Ni, buffer stock solutions, and a protocol. The detergents are supplied in aqueous stock solutions, eliminating the handling of dry powder detergents. Membrane Protein Purification Kit is sufficient for two complete screening experiments according to the included protocol.

ordering informationProduct code number

Membrane Protein Purification Kit 28-9805-82

For more information, visit www.gelifesciences.com/protein-purification

Mag Sepharose beads are magnetic beads designed for simplified enrichment of target proteins by immunoprecipitation, pull-down applications, or enrichment of phosphopeptides using titanium dioxide chromatography. Captured protein and peptide fragment targets are immobilized to ligands attached to Mag Sepharose beads and collection of beads and captured protein is achieved using a magnet.

Two new products have been added to the range of Mag Sepharose beads:

Protein A Mag sepharose Xtra and Protein G Mag sepharose Xtra are designed for efficient, high capacity small-scale purification/screening of monoclonal and polyclonal antibodies from various species. Antibodies are captured with high specificity on protein A and protein G ligands attached to Mag Sepharose. Protein A and Protein G Mag Sepharose beads allow simple capture of antibodies from low microliter to high milliliter scales. Purification of more than 350 µg of human IgG per purification run is possible.

His Mag sepharose ni is designed for efficient, small-scale purification/screening of histidine-tagged proteins. Histidine-tagged proteins are captured on the Ni2+ ligand coupled to Mag Sepharose magnetic beads. Purification of up to 500 µg of histidine-tagged protein per purification is possible.

The high density of the beads allows rapid capture with magnetic devices while the visibility of the beads ensures reliable collection of the target protein. In addition, the beads are hydrophilic and nonadherent and this reduces smearing effects and aggregate formation. Together with MagRack 6, a separation tool for handling the beads in microcentrifuge tubes, up to six samples can be processed in parallel. Large numbers of samples can be screened in parallel and throughput increased even further with the use of a robotic instrument.

expanded range of Mag sepharose™ beads

ordering informationProducts code number

Protein A Mag Sepharose Xtra, 2 × 1 ml 28-9670-56

Protein A Mag Sepharose Xtra, 5 × 1 ml 28-9670-62

Protein G Mag Sepharose Xtra, 2 × 1 ml 28-9670-66

Protein G Mag Sepharose Xtra, 5 × 1 ml 28-9670-70

His Mag Sepharose Ni, 2 × 1 ml 28-9673-88

His Mag Sepharose Ni, 5 × 1 ml 28-9673-90

His Mag Sepharose Ni, 10 × 1 ml 28-9799-17

For more information, visit www.gelifesciences.com/sampleprep

Strategies for Protein Purification is an all new handbook focusing on chromatographic purification of proteins at research scale. Beginners can use the handbook to get a quick start when entering the field; experienced protein purifiers will also find valuable information.

Strategies for Protein Purification describes how to design a protein purification procedure, how to select and combine chromatography methods and purification formats, and how to monitor and evaluate protein purification. It gives an overview of the available methods and provides advice on how to avoid pitfalls in all operations, from initial sample preparation to final analysis of the purified protein.

The handbook complements the existing series of handbooks from GE Healthcare that describe the different chromatographic purification methods in detail.

the code number for ordering a printed copy of the handbook is 28-9833-31, or download the PDF at www.gelifesciences.com/handbooks

strategies for Protein Purification – new handbook

This handbook describes the use of gel filtration (size-exclusion chromatography) for the purification and separation of biomolecules, with focus on practical information for obtaining the best results.

The first step towards a successful separation is to select the correct gel filtration medium for your specific application. To assist you in making this choice, the content of this handbook has been reorganized with new chapters devoted to how to use GE Healthcare’s Superdex™, Superose™, Sephacryl™, and Sephadex™ gel filtration media and prepacked columns.

Further, the handbook includes detailed instructions for the most common applications, the theoretical principles behind the technique, as well as advice on sample preparation, desalting, column packing, and much more.

the code number for ordering a printed copy of the handbook is 18-1022-18, or download the PDF at www.gelifesciences.com/handbooks

Gel Filtration Principles and Methods – updated handbook

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This handbook describes the methodology for using gradients of Ficoll-Paque™ media for density centrifugation of human mononuclear cells. Methodology and applications of the sterile, density gradient media Ficoll-Paque PLUS and Ficoll-Paque PREMIUM are described in detail. These media of 1.077 g/ml density are supplied ready-to-use for the purification of mononuclear cells from human peripheral blood.

This update further introduces the use of Ficoll-Paque PREMIUM 1.073 and 1.084, which are centrifugation media for the isolation of lower- density cells such as monocytes and higher-density mononuclear cells, respectively. Ficoll-Paque PREMIUM products are based on Ficoll-Paque PLUS but are manufactured in a GMP-compliant environment and under a Quality Management System certified to ISO 13485:2003. Ficoll-Paque PREMIUM can be incorporated into existing procedures where Ficoll-Paque PLUS is used, enabling the isolation of human mononuclear cells from bone marrow, peripheral blood, and umbilical cord blood.

Further, the reference list in the handbook has been updated to now include more recent journal citations on the use of Ficoll-Paque products.

the code number for ordering a printed copy of the handbook is 18-1152-69, or download the PDF at www.gelifesciences.com/handbooks

Isolation of mononuclear cells – updated handbook

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?ÄKTA avant 150 is a preparative liquid chromatography system for achieving fast preparative chromatography and secure process development. UNICORN™ 6 control software has been specially developed for ÄKTA avant to increase productivity and facilitate easy scale-up. The ÄKTA avant system offers a complete solution for fast, high-quality protein separations while maintaining flexibility and reliability.

ÄKTA avant 150 complements ÄKTA avant 25, which is designed for chromatography media screening and method optimization using small columns. ÄKTA avant 150 is designed for scaling up to larger columns, as well as fine-tuning and robustness testing of the optimized process.

HiScale is a new range of pressure-stable, empty columns designed for preparative chromatography, scale-up, and process development. The columns are available in a number of sizes suited to larger scale chromatography applications with a number of features to allow easy packing.

HiScale columns provide:

> reproducible and scalable purifications: Axial compression packing and novel plunger mechanism enables flexible packing protocols

> rapid, trouble-free operation: Easy handling and cleaning with QuickLock adapter mechanism and fully compatible with XK column methods

> High pressure stability: Pressure stable up to 20 bar (290 psi) for scale-up and process development

For more information, visit www.gelifesciences.com/akataavant and www.gelifesciences.com/hiscale

ÄKtA™ avant 150 and Hiscale™ columns for preparative chromatography and process development

expanded range of Hiscreen™ columns for purification and process development

The HiScreen column line has been extended with four new columns—HiScreen Blue FF, HiScreen Ni FF, HiScreen DEAE FF, and HiScreen Butyl HP. The different functional ligands of the new columns allow purification by affinity (AC), ion exchange (IEX), or hydrophobic interaction chromatography (HIC), respectively.

The four new columns are:

> Hiscreen Blue FF: Prepacked with Blue Sepharose™ Fast Flow containing the Cibacron™ Blue 3G ligand to enable purification or removal of proteins such as albumin, interferon, lipoproteins, and blood coagulation factors.

> Hiscreen ni FF: Prepacked with Ni Sepharose 6 Fast Flow immobilized with a Ni2+ charged ligand for capture and purification of histidine-tagged proteins

> Hiscreen DeAe FF: Prepacked with DEAE Sepharose Fast Flow, which contains the weak anion exchanger group DEAE for protein capture applications by IEX.

> Hiscreen Butyl HP: Prepacked with Butyl Sepharose High Performance, which allows high-resolution polishing applications by HIC.

HiScreen columns have small bed volumes (4.7 ml) for fast results and minimal sample/buffer consumption. The columns have a 10 cm bed height and capacity can be increased by connecting columns in series to achieve a 20 cm bed height.

Find out more about all 23 available Hiscreen columns at www.gelifesciences.com/protein-purification

ordering informationProducts code number

HiScreen Blue FF, 1 × 4.7 ml 28-9782-43

HiScreen Ni FF, 1 × 4. 7 ml 28-9782-44

HiScreen DEAE FF, 1 × 4.7 ml 28-9782-45

HiScreen Butyl HP, 1 × 4.7 ml 28-9782-42