BIO 205 Chapter 4 Powerpoint

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    BIO 205

    Chapter 4

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    Chlamydiae are extremely small, mostly pathogenic bacteria

    Cyanobacteria carry out photosynthesis using chlorophyll,responsible for the appearance of oxygen in Earths earlyatmosphere

    Actinobacteria include Streptomyces, the genus that produces theantibiotic streptomycin

    Firmucutes consist of many gram-positive species, such asBacillus, Clostridium, Staphylococcus, and Streptococcus

    Proteobacteria contain the largest and most diverse group ofspecies, including E. coliand many human pathogens

    The majority of the 18 phyla ofBacteria play a positive role innature

    The Domain Bacteria Contains Some of the Best Studied Prokaryotes

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    Thermoacidophiles (grow athigh temperature with low

    pH

    Extreme halophiles (saltlovers)

    Methanogens (live inanoxic environments)

    Euryarchaeota contain:

    The DomainArchaea ContainsMany Extremophiles

    Crenarchaeota tend to grow inhot acidic environments suchas hot springs and volcanic

    vents

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    The Shapes and Arrangements of Prokaryotic Cells

    Three Main Shapes:

    Rod

    Sphere

    Spiral

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    The Shapes and Arrangements of Prokaryotic Cells

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    Streptococcus Chain

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    Many cocci and some bacilli appear in groups or chains

    A spherical bacterial cell is a coccus

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    Spiral shaped bacteria can appear as vibrios (comma-shaped), spirilla (helical), or spirochete

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    Prokaryotic Cell

    Structure

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    Pili

    Pili help:

    Pili Are Protein Fibers Extending from the Surfaceof Many Prokaryotes

    Form biofilms andmicrocolonies.Some bacterialspecies have

    conjugation pili,used to transfergenetic materialbetween cells

    Attach cells to surfaces

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    Prokaryoticflagella contain ahelical filament,a hook, and a

    basal body

    Flagella can be used for cell motility

    Prokaryotic Flagella Are Long Appendages

    Extending from the Cell Surface

    Flagella

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    Flagella Continued

    Bacteria exhibit chemotaxis, moving up the concentrationgradient of a chemical attractant

    Prokaryotic Flagella Are Long Appendages Extending fromthe Cell Surface

    Some prokaryotes move without flagella by gliding across a

    solid surface

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    It protects cells from theenvironment, and allows them

    to attach to surfaces

    A thin diffuse layer ofpolysaccharides is a slime layer

    A thick glycocalyx covalentlybound to the cell is a capsule

    The glycocalyx is an adheringlayer of polysaccharides (andsometimes small proteins)

    The Glycocalyx Is an Outer LayerExternal to the Cell Wall

    Glycocalyx

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    CapsuleCapsule in Acinetobacter species by negative staining

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    Cell Envelope

    The cell wall protectsthe cell from injury, andmaintains cell shapeand water balance

    Cell Membrane

    Cell Wall

    The cell envelope iscomposed of two

    parts:

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    Prokaryotic Cell Wall

    The outer membrane contains proteins called porinsthat selectively allow small molecules into the

    periplasmic space

    The gram-negative cell wall has an outer membrane,separated from the cell membrane by the periplasmic

    space

    Gram-negative bacteria have a two-dimensional

    peptidoglycan layer and no teichoic acid

    Gram-positive bacteria have thick peptidoglycan cellwalls containing teichoic acid

    Cell walls in prokaryotes contain peptidoglycan

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    The S-layer is the most common archaeal cell wall,consisting of protein or glycoprotein in a crystal lattice

    Others have polysaccharides, proteins, or both

    No archaea have peptidoglycan in the cell wall, but somehave pseudopeptidoglycan

    The Archaeal Cell Wall Also Provides Mechanical Strength

    Archaeal Cell Wall

    P k ti C ll M b

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    Prokaryotic Cell Membrane

    Hydrophobic fatty acid chains in the phospholipids form a

    permeability barrier

    The phospholipid molecules are arranged in a bilayer

    The cell membrane is a fluid layer of phospholipid and

    protein (the fluid mosaic model)

    The Cell Membrane Represents the Interface between theCell Environment and the Cell Cytoplasm

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    molecule transport

    sensation of stimuli

    DNA replication

    energy metabolism

    cell wall synthesis

    Membrane proteins perform or aid in many functions,such as:

    Antimicrobial substances may disrupt or dissolve thebilayer

    The Cell Membrane Represents the Interface between theCell Environment and the Cell Cytoplasm

    Prokaryotic Cell Membrane

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    Facilitated Diffusion

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    Adjacent lipid tails are bound together forming a lipidmonolayer, instead of a bilayer

    Fatty acids are usually absent

    Hydrophobic lipid tails are attached to glyceroldifferently in archaea

    The Archaeal Cell Membrane Differs from Bacterial andEukaryotic Membranes

    Archaeal Cell Membranes

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    The Cell Cytoplasm and Internal Structures

    N l id

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    The nucleoid is a central subcompartment in the cytoplasmwhere DNA aggregates

    The nucleoid represents a subcompartment containing thechromosome

    Nucleoid

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    Most cells have only one copy of each gene(are haploid), so cannot undergo mitosis likeeukaryotes

    The complete set of genes is called thegenome

    The DNA contains the genes (hereditaryinformation)

    The chromosome is usually a closed loop ofDNA and protein

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    R plasmids carry genes for resistance to

    antibiotics

    Plasmids can be transferred between cells and

    can be used as vectors in genetic engineering

    Each plasmid is a closed loop, containing 5-10genes

    Plasmids are molecules of DNA smaller thanthe chromosome

    Plasmids Are Found in Many Prokaryotic Cells

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    Magnetosomes contain crystals of magnetite or greigite, allowingcells to respond to magnetic fields

    Some aquatic bacteria use gas vesicles to float on the waterssurface

    Inclusion bodies store nutrients or building blocks for cellularstructures

    There are hundreds of thousands of ribosomes, used for proteinsynthesis

    Other Subcompartments Exist in the Prokaryotic Cytoplasm

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    The Prokaryotic Cell

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    End of Chapter 4