Université d’Ottawa / University of Ottawa

BIO2135 Animal Form and Function1

Lab orientation, magnification and Lab orientation, magnification and surface to volume ratio in animalssurface to volume ratio in animalsLab orientation, magnification and Lab orientation, magnification and surface to volume ratio in animalssurface to volume ratio in animals

BIO2135 Animal Form and Function

Université d’Ottawa / University of OttawaUniversité d’Ottawa / University of Ottawa

BIO2135 Animal Form and Function2

Today’s objectivesToday’s objectivesToday’s objectivesToday’s objectives Logging on and off the computers BIOLabo– Setting up bright field alignment

(Compound microscope)– Setting up the stereoscope

Image preview and capture (Image J software)

Magnification– Fields of view

Logging on and off the computers BIOLabo– Setting up bright field alignment

(Compound microscope)– Setting up the stereoscope

Image preview and capture (Image J software)

Magnification– Fields of view

BIO2135 Animal Form and Function

Université d’Ottawa / University of OttawaUniversité d’Ottawa / University of Ottawa

BIO2135 Animal Form and Function3

Objectives (Cont.)Objectives (Cont.)Objectives (Cont.)Objectives (Cont.) Understand factors affecting diffusion

rates. Understand how surface to volume ratio

is affected by the size of the animal Present quantitative data with a graph

and caption Understand the role of data

transformation, display error in measurements

Gather and report the information

Understand factors affecting diffusion rates.

Understand how surface to volume ratio is affected by the size of the animal

Present quantitative data with a graph and caption

Understand the role of data transformation, display error in measurements

Gather and report the information

BIO2135 Animal Form and Function

Université d’Ottawa / University of OttawaUniversité d’Ottawa / University of Ottawa

BIO2135 Animal Form and Function4

Administrative informationAdministrative informationAdministrative informationAdministrative information

Contact information Care and proper storage of

microscopes

Contact information Care and proper storage of

microscopes

BIO2135 Animal Form and Function

Université d’Ottawa / University of OttawaUniversité d’Ottawa / University of Ottawa

BIO2135 Animal Form and Function5

Science networkScience networkScience networkScience network

Digital Diversity – open your copy Applications Launcher–BIOLabo

–Digital Zoology for when needed

–BIO2135 Website– ImageJ

Digital Diversity – open your copy Applications Launcher–BIOLabo

–Digital Zoology for when needed

–BIO2135 Website– ImageJ

BIO2135 Animal Form and Function

Université d’Ottawa / University of OttawaUniversité d’Ottawa / University of Ottawa

BIO2135 Animal Form and Function6

MagnificationMagnificationMagnificationMagnification

BIO2135 Animal Form and Function

Université d’Ottawa / University of OttawaUniversité d’Ottawa / University of Ottawa

BIO2135 Animal Form and Function7

Magnification Magnification specimensspecimensMagnification Magnification specimensspecimens

Tetrahymena Spirostomum Planaria Liver fluke

(Fasciola hepatica) Earthworm Sea urchin Perch Turtle Yourself

Tetrahymena Spirostomum Planaria Liver fluke

(Fasciola hepatica) Earthworm Sea urchin Perch Turtle Yourself

Measurement Measurement specimensspecimens

Measurement Measurement specimensspecimens

Taenia pisiformis Amphioxus Blepharisma Aurelia ephyra Crayfish Planaria Liver fluke (Fasciola hepatica) Paramecium

Taenia pisiformis Amphioxus Blepharisma Aurelia ephyra Crayfish Planaria Liver fluke (Fasciola hepatica) Paramecium

BIO2135 Animal Form and Function

Université d’Ottawa / University of OttawaUniversité d’Ottawa / University of Ottawa

BIO2135 Animal Form and Function8

Saving a Picture: Dos and Don’tsSaving a Picture: Dos and Don’tsSaving a Picture: Dos and Don’tsSaving a Picture: Dos and Don’ts Save file to K: drive in folder for Bio2135A2

My Documents on ‘sci.uottawa.ca\homes\Undergrads\bio312d4’(K:)

Save only as .jpgOther file formats will not be transferred

DON’T create subfolders in the Bio2135A2 foldere.g. contents Bio2135A2\Lab 1 will NOT be transferred!

Save file to K: drive in folder for Bio2135A2 My Documents on ‘sci.uottawa.ca\homes\Undergrads\bio312d4’(K:)

Save only as .jpgOther file formats will not be transferred

DON’T create subfolders in the Bio2135A2 foldere.g. contents Bio2135A2\Lab 1 will NOT be transferred!

BIO2135 Animal Form and Function

Université d’Ottawa / University of OttawaUniversité d’Ottawa / University of Ottawa

BIO2135 Animal Form and Function9

Measuring surface Measuring surface to volume ratiosto volume ratiosMeasuring surface Measuring surface to volume ratiosto volume ratios

All measurements in mm!Data recorded in spreadsheeton the designated computers

BIO2135 Animal Form and Function

Université d’Ottawa / University of OttawaUniversité d’Ottawa / University of Ottawa

BIO2135 Animal Form and Function10

Microscopy measurementMicroscopy measurementMicroscopy measurementMicroscopy measurement Snap and save your picture using image J.

Open ruler in imageJ. Be sure both are at the same zoom.

Snap and save your picture using image J. Open ruler in imageJ. Be sure both are at the same zoom.

40x

BIO2135 Animal Form and Function

Université d’Ottawa / University of OttawaUniversité d’Ottawa / University of Ottawa

BIO2135 Animal Form and Function11

Thought provoking questions for Thought provoking questions for discussiondiscussionThought provoking questions for Thought provoking questions for discussiondiscussion

What things are moved (exchanged) across the surfaces of organisms?

Where are the gas, nitrogenous waste, and nutrient exchange surfaces in each organism?

Gas exchange requires a moist permeable surface how do organisms balance this requirement with water loss and how does where they live affect this?

What prevents stagnation at the exchange surface? Are there ways to increase the concentration of the material

being exchanged either inside or outside the animal?

What things are moved (exchanged) across the surfaces of organisms?

Where are the gas, nitrogenous waste, and nutrient exchange surfaces in each organism?

Gas exchange requires a moist permeable surface how do organisms balance this requirement with water loss and how does where they live affect this?

What prevents stagnation at the exchange surface? Are there ways to increase the concentration of the material

being exchanged either inside or outside the animal?

BIO2135 Animal Form and Function

Université d’Ottawa / University of OttawaUniversité d’Ottawa / University of Ottawa

BIO2135 Animal Form and Function12

TimelineTimelineTimelineTimeline

Dr. Houseman’s Prelab (10 min) Administrative Prelab (15 min) TA Prelab (15 min) Biolabo and magnification (50 min) Surface to volume (30 min) Group discussion (10 min) Class discussion (30 min)

Dr. Houseman’s Prelab (10 min) Administrative Prelab (15 min) TA Prelab (15 min) Biolabo and magnification (50 min) Surface to volume (30 min) Group discussion (10 min) Class discussion (30 min)

BIO2135 Animal Form and Function

Université d’Ottawa / University of OttawaUniversité d’Ottawa / University of Ottawa

BIO2135 Animal Form and Function13

Graph Assignment using class dataGraph Assignment using class data(You plot all individual data points– each student submits a (You plot all individual data points– each student submits a report, this is not a team assignment)report, this is not a team assignment)

Graph Assignment using class dataGraph Assignment using class data(You plot all individual data points– each student submits a (You plot all individual data points– each student submits a report, this is not a team assignment)report, this is not a team assignment) Graph #1- Surface Area as a function of volume

Graph #2 - Log10 (Surface Area) as a function of Log10 (volume). Slope, standard error, 95% confidence calculations and conclusion.

Graph #3 - Log10 (Surface Area:Volume) as a function of Log10 (volume). Slope, standard error, 95% confidence calculations and conclusions.

Graph #1- Surface Area as a function of volume

Graph #2 - Log10 (Surface Area) as a function of Log10 (volume). Slope, standard error, 95% confidence calculations and conclusion.

Graph #3 - Log10 (Surface Area:Volume) as a function of Log10 (volume). Slope, standard error, 95% confidence calculations and conclusions.

BIO2135 Animal Form and Function

Université d’Ottawa / University of OttawaUniversité d’Ottawa / University of Ottawa

BIO2135 Animal Form and Function14

ReportReportReportReport Data and instructions will be

provided January 23, 2012 on the Bio2135 web site (Excel and pdf files).

Due date will be January 30, 2012 before 4:00 pm in the document drop-off room box for your lab section.

Data and instructions will be provided January 23, 2012 on the Bio2135 web site (Excel and pdf files).

Due date will be January 30, 2012 before 4:00 pm in the document drop-off room box for your lab section.