CESAM – Lisboa Environmental Biology and Health

Subgroup

Maria Filomena Caeiro

Fevereiro de 2013

Nucleo-cytoplasmic large DNA viruses (NCLDVs) and their

pathogenic potencial

In collaboration with António Pedro Alves de Matos (CESAM) & Rachel

Marschang (Hohenheim University, Stuttgart, Germany)

Characterization of ranaviruses isolated from the Portugese fauna

Ana Mafalda Dinis

Master thesis on Applied Microbiology (concluded)

Nucleo-cytoplasmic large DNA viruses: detection and characterization of

viruses in environmental amoeba

Mariana Costa Fugas

Master thesis on Molecular Biology and Genetics (concluded)

Mara Maeva Meirsman & Vera Mónica Brito Salgado

IIM2 – 2nd semester Master on Applied Microbiology (underway)

Ana Mendes & Maria Silva

Undergraduate students

Alves De Matos A.P., Caeiro M.F., Papp T., Matos B.A., Correia A.C. & R.E. Marschang (2011). New viruses from Lacerta

monticola (Serra da Estrela, Portugal): further evidence for a new group of nucleo-cytoplasmic large deoxyriboviruses

(NCLDVs). Microscopy and Microanalysis 17: 101–108 and CJO 08 Dec 2010 (Doi:10.1017/S143192761009433X).

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Cinéticas dos ciclos replicativos dos isolados de ranavírus portugueses

e do FV3

A B C

D E F

G H

Figure 1 – Free living amoeba isolated from AW1, AW2, Granjal,

Ferragudo, LO1 and LO2 collected samples. All images were

obtained in bright field. The amoebas were termed after the sample

from where they were isolated: A - AW1 trophozoite; B – AW2

trophozoite; C – GH trophozoite; D – GP trophozoite; E – Fer

trophozoite; F – LO1 trophozoite; G – LO2 cyst; H – LO2 trophozoite.

Figure 2 – Free living amoeba isolated from the Tagus River region.

All images were obtained in bright field. The amoebas were named by

numbers corresponding to a specific sample and media in which they

grew (see Table 2): A – 1 trophozoite; B – 2 cyst; C – 2 trophozoite; D –

3 cyst; E – 3 trophozoite; F – 4 trophozoite and cyst; G – 5 trophozoite; H

– 6 cyst.

A B C

D E F

G H

Detection, isolation and characterization of viruses

involved in human and animal health

Detection and characterization of viruses circulating in Portuguese

populations of Alectoris rufa and other game birds. In collaboration with Deodália Dias (CESAM)

Maryline Dias

Master thesis on Human Biology and Environment (concluded)

• Paper under submission: • Coronaviruses detection in healthy Alectoris rufa in Portuguese breeding properties. Dias M., Dias D. & Caeiro M.F.

Looking for viruses in animal tissues from natural populations of

rodents In collaboration with Cristiane Bastos-Silveira (CESAM)

Alexandra Carvalho

Master thesis on Applied Microbiology (concluded)

• Attempts on the isolation of the Portuguese EMCV in cell cultures

• Paper under submission: • First molecular detection of Encephalomyocarditis virus in a Lusitanian pine vole (Microtus lusitanicus) in

Portugal. Carvalho A., Cerveira A., Bastos-Silveira C. & Caeiro M.F.

Studies on antiviral activities of aqueous plant extracts In collaboration with Lia Ascenção (CBV) & Luísa Serralheiro (CQB) &

António Pedro Alves de Matos (CESAM)

Fernanda Resende

Master thesis on Applied Microbiology (concluded)

Nadieny Barbosa

Master thesis on Molecular Biology and Genetics (undeway)

Mónica Barra

Master thesis on Applied Microbiology (underway)

• Virucidal effect against:

Herpes simplex viruses (Type 1 and type 2)

Encephalomyocarditis virus (EMCV)

Ranaviruses (FV3 and Portuguese isolates from

diseased animals: Triturus marmuratus and

Triturus boscai)

• Effect on the replication cycles

Resultados Citotoxicidade

Resultados Estudo da atividade anti-herpética – Efeito virucida

Resultados Efeito no ciclo replicativo (tempos de adição)

Representação gráfica (Microsoft Excel) da influência do tempo de adição dos extratos (CMNC),

durante a adsorção e a diferentes tempos pós infeção, na percentagem de inibição da produção de

partículas virais infecciosas (HSV-1, HSV-2).

Molecular methods applied to fungal identification

and detection

Diversity of marine fungi on the Portuguese west coast

In collaboration with Margarida Barata (CBA)

Egídia Azevedo

PhD thesis (concluded)

- Azevedo E., Rebelo R., Caeiro M. F. & Barata M. (2010). Diversity and Richness of marine fungi on two

Portuguese marinas. Nova Hedwigia. 90 (3-4): 521- 531

- Azevedo A., Caeiro M.F., Rebelo R. & Barata M. (2011) Biodiversity and characterization of marine mycota from

Portuguese waters. Animal Biodiversity and Conservation 34.1: 205-215

- Azevedo E., Rebelo R., Caeiro M.F. & Barata M. (2012). Use of drift substrates to characterize marine fungal

communities from the west coast of Portugal. Mycologia 2012 11-191; Published online: January 12, 2012,

doi:10.3852/11-191 (in press)

- Azevedo E., Barata M., Marques M.I.& Caeiro M.F. Lignicolous Lulworthia spp. isolated in Portuguese waters:

characterization and phylogenetic analysis (under submission)

Rhizospheric microbial consortia to increase nutrient use efficiency. A tool

to be used in intensive farm systems (PTDC/AGR-PRO/115888/2009)

In collaboration with Cristina Cruz (CBA) - PI of the Project

Manuscript in preparation: • Next generation sequencing for metagenomics of endophytic fungi. Delgado M., Antunes P., Cruz C.

& Caeiro M.F.

PHYLOGENETIC TREE

FOR PORTUGUESE

LULWORTHIALES

Sequences: •Aligned with Clustalw

•Phylip analysis:

•Dnadist

•Njtree

•1000 bootstrap

Azevedo E., Barata M.

Marques M.I. & Caeiro M.F.

(under submission)

Endophytic fungi of maize:

Metagenomic analysis

Delgado M., Antunes P.,

Cruz C. & Caeiro M.F.

(2013) in preparation

Delgado M., Antunes P.,

Cruz C. & Caeiro M.F.

(2013) in preparation

Endophytic fungi of maize:

Metagenomic analysis