Biology 3 Lab Updated 9/6/13

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Biology 3 Lab Updated 9/6/13

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Biology 3 Lab Updated 9/6/13

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Biology 3 Lab Updated 9/6/13

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Biology 3 Lab Updated 9/6/13

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Biology 3 Lab Updated 9/6/13

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Biology 3 Lab Updated 9/6/13

Review Questions Regarding Laboratory Techniques: Safety Precautions

1. In this laboratory, where is the nearest:

(a) Fire extinguisher? ________________________________________________

(b) Safety shower? _________________________________________________

(c) Eyewash Fountain? ______________________________________________

(d) Fire Alarm? ____________________________________________________

(e) Telephone? ____________________________________________________

(f) Fire Blanket? ___________________________________________________

2. What does the acronym MSDS stand for? What does it mean?

______________________________________________________________________________ ______________________________________________________________________________ ______________________________________________________________________________

3. Briefly explain the prerequisite process for the disposal of excess materials? ______________________________________________________________________________ ______________________________________________________________________________ ______________________________________________________________________________

4. List all the defects that make glassware unusable? ______________________________________________________________________________ ______________________________________________________________________________ ______________________________________________________________________________ ______________________________________________________________________________ ______________________________________________________________________________ ______________________________________________________________________________ ______________________________________________________________________________ ______________________________________________________________________________

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Biology 3 Lab Updated 9/6/13

5. Briefly describe the precautions you should take when you mix chemicals in a test tube? ______________________________________________________________________________ ______________________________________________________________________________ ______________________________________________________________________________

6. Briefly describe the manner in which you protect your hands when inserting glass tubing into a rubber stopper?

______________________________________________________________________________ ______________________________________________________________________________ ______________________________________________________________________________

7. What do the upper and right segments of the NFPA symbolize?

(a) _____________________________________________________________________ ____________________________________________________________________________________________________________

(b) _____________________________________________________________________ ____________________________________________________________________________________________________________

8. Briefly describe the proper technique for heating liquids in a test tube to temperatures below 100 0C?

______________________________________________________________________________ ______________________________________________________________________________ ______________________________________________________________________________ ______________________________________________________________________________

9. Briefly describe what you should do first if you burn a small area on your skin in the laboratory? ______________________________________________________________________________ ______________________________________________________________________________ ______________________________________________________________________________

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Biology 3 Lab Updated 9/6/13

10. What should you do immediately if you notice that your clothing is starting to burn? ______________________________________________________________________________ ______________________________________________________________________________ ______________________________________________________________________________ ______________________________________________________________________________ ______________________________________________________________________________ ______________________________________________________________________________

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Biology 3 Lab Updated 9/6/13

Glassware and General equipment

Laboratory apparatus used in both general chemistry and biology can be

divided into two broad categories: glassware and equipment. Each piece of apparatus is designed to be used for one or more specific purposes. Figures 5(a) on pages 12 and 13 show illustrations, names, uses and sizes of most glassware and general equipment. Be sure you know the names and uses of each item shown.

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Glassware Questions

1. Which is the best piece of equipment(s) to use to measure exactly 5.0 mL of a liquid? ______________________________________________________________________________ ______________________________________________________________________________

2. Which piece of equipment(s) should you use to measure exactly 2.75 mL of a liquid? ______________________________________________________________________________ ______________________________________________________________________________

3. Which is the best piece of equipment(s) should you use to remove a red-hot crucible from a clay triangle?

______________________________________________________________________________ ______________________________________________________________________________

4. Describe which is the best method and heat source to use to heat a flammable liquid? ______________________________________________________________________________ ______________________________________________________________________________

5. Describe which is the best method and which piece of equipment should you use to heat a solid to a high temperature?

______________________________________________________________________________ ______________________________________________________________________________ ______________________________________________________________________________

6. What type of glassware should you use to prepare exactly 250 mL of a standard solution?

______________________________________________________________________________ ______________________________________________________________________________

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7. Describe which method is the best and which piece of equipment should you use to hold

a test tube over a Bunsen burner flame? ______________________________________________________________________________ ______________________________________________________________________________ ______________________________________________________________________________ ______________________________________________________________________________

8. Name three types of glassware that you can use to evaporate a solution?

(1) _____________________________________________________________________ (2) _____________________________________________________________________

(3) _____________________________________________________________________

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