Biology Catalase Experiment

8/20/2019 Biology Catalase Experiment

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Nimisha Sharma

Biology

IB1

Efect o the substrate concentration on enzymecatalysed reaction

Aim: To investigate how does changing the substrate concentration of

hydrogen peroxide aect the rate of reaction whereby hydrogen peroxide

is broken down to oxygen gas and water by the enzyme catalase,

measured by the volume of oxygen gas released in 30 seconds.

Raw ata

!able 1" Showing #olume o $%ygen released at diferent

concentrations o hydrogen &ero%ide o#er '( seconds)

*oncentra

tion o

+,$, -

./1)(0

olume o $, released2cm' / ()3

!rial

s

3)(2sec

/ ()3

1()(2se

c/ ()3

13)(2se

c/ ()3

,()(2se

c/ ()3

,3)(2se

c/ ()3

'()(2se

c/ ()3

'31 3)( ,3)( 4,)( 51)( 67)( 88)(, 1')( '()( '4)( '6)( '7)( 41)(' 17)( 4()( 48)( 3')( 34)( 33)(

'(

1 ,)( 18)( 4,)( 53)( 68)( 83)(, ')( ,6)( '4)( 6,)( 8,)( 83)(' 7)( ,()( 48)( 63)( 88)( 74)(

,31 ')( 5)( 13)( ,')( '5)( 3,)(, 4)( 3)( 7)( 1')( ,')( 4,)(' 4)( 5)( 1()( 1,)( '4)( 34)(

,(

1 ,)( 4)( 3)( 1()( 14)( 17)(

, ')( 5)( 1()( 15)( ,4)( ,3)(' 6)( 7)( 1,)( 16)( ,3)( '1)(

131 ')( 3)( 6)( 1()( 1,)( 13)(, ')( 5)( 1()( 14)( 18)( ,')(' ')( 4)( 3)( 5)( 8)( 11)(

1(1 ()( ()( ()( ()( ()( ()(, ')( 4)( 4)( 5)( 6)( 7)(' 4)( 4)( 3)( 5)( 5)( 6)(

31 ()( ()( ()( ()( ()( ()(, ,)( ')( 4)( 5)( 6)( 7)(

' ')( ')( 3)( 5)( 5)( 6)(


2/9

ata 9rocessing

!able ," Showing a#erage #olume o o%ygen released at each

concentration o hydrogen &ero%ide

olume o $,2cm' /()3+,$,*oncentrat

ion - ./

1)(0

3)(2s

ec/

()3

1()(2s

ec/

()3

13)(2s

ec/

()3

,()(2s

ec/

()3

,3)(2s

ec/

()3

'()(2s

ec/

()3

!ria

ls

'3

1

1,)' '1)6

41)'

3()'

36)'

51)','

'(

1

4)6

,1)6

4()6

6()6

8,)6

88)(,'

,3

1

')6 3)6

11)'

15)(

'1)(

47)','

,(

1

4)( 5)'

7)(

14)'

,1)(

,3)(,

'

1

')(

3)(

6)' 1()(

1,)6

15)',

'

1(

1

,)'

,)6

')6

4)6

3)6

5)6,

'

3

1

1)6

,)(

')(

4)(

4)' 3)',

'

!able '" Showing the Error bars .a%"in2,0

olume o $,2cm'

/()3+,$, 3)(2s 1()(2s 13)(2s ,()(2s ,3)(2s '()(2s


3/9

*oncentrat

ion - ./

1)(0

ec/

()3

ec/

()3

ec/

()3

ec/

()3

ec/

()3

ec/

()3

'3!rial 1

!rial ,

!rial '

6)( 6)3 6)( 1,)( ,()( ,')3

'(!rial 1

!rial ,

!rial '

')3 4)3 1()( 3)( 3)( 4)3

,3!rial 1

!rial ,

!rial '

()3 ()3 ')( 3)3 5)3 5)(

,(!rial 1

!rial ,

!rial '

,)3 ,)3 ')3 ')3 3)3 5)(

13!rial 1

!rial ,

!rial '

()( 1)( ,)3 4)( 3)( 5)(

1(!rial 1

!rial ,!rial '

,)( ,)( ')( 4)( 3)( 5)(

3!rial 1

!rial ,

!rial '

1)3 1)3 ,)3 ')( ')3 4)3

The data collected above had to be processed in order to draw a

meaningful conclusion from it. irst, ! found the mean volume of "xygen

released for 3 trials of each dierent substrate concentration. Then !calculated the error bars #range$ around the mean for all the trials of each

dierent substrate concentration.

Sam&le calculations

1) %alculation of average &olume of the "xygen produced ' Sum o all

trials2No) o trials,) %alculation of error bars #range$ of the volume of oxygen produced' a%"

in2, .cm'0') The gradient in each graphs is found by using the (icrosoft )xcel on the

computer after generating the graphs.


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;ra&h 1

0 * +0 +* ,0 ,* 30 3*0

+

,

3

-

*

f#x$ ' 0.+/x

1 ' 0.22

&olume of oxygen produced in thirty seconds when the concentration of hydrogen peroxide is *

Time4seconds5+

&olume of oxygen4cm6

;ra&h ,

0 * +0 +* ,0 ,* 30 3*0

+

,

3

-

*

7

/

f#x$ ' 0.,3x

1 ' 0.22

&olume of oxygen produced in thirty seconds when the concentration of hydrogen peroxide is +0

Time4seconds 5+

&olume of "xygen4cm6


5/9

;ra&h '

0 * +0 +* ,0 ,* 30 3*0

*

+0

+*

,0

f#x$ ' 0.*,x1 ' +

&olume of oxygen produced in thirty seconds when the concentration of hydrogen peroxide is +*

Time4seconds 5+


;ra&h 4

0 * +0 +* ,0 ,* 30 3*0

*

+0

+*

,0

,*

30

f#x$ ' 0.7/x

1 ' 0.22

&olume of oxygen produced in thirty seconds when the concentration of hydrogen peroxide is ,0

Time4seconds 5+



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;ra&h 3

0 * +0 +* ,0 ,* 30 3*0

+0

,0

30

-0

*0

0

f#x$ ' +.,-x

1 ' 0.2+

&olume of oxygen produced in thirty seconds when the concentration of hydrogen peroxide is ,*

Time4seconds 5 +

&oume of "xygen4cm6

;ra&h 5

0 * +0 +* ,0 ,* 30 3*0

+0

,0

30

-0

*0

0

70

/0

20

+00

f#x$ ' 3.0x

1 ' 0.2/

&olume of oxygen produced in thirty seconds when the concentration of hydrogen peroxide is 30

Time/seconds ±1



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;ra&h 6

0 * +0 +* ,0 ,* 30 3*0

+0

,0

30

-0

*0

0

70f#x$ ' ,.3,x

1 ' 0.2/

&oume of "xygen produced in thirty seconds when the concentration of hydrogen peroxide is 3*

Time4seconds 5+


9rocessed data

!able 4" Showing the gradients ound in the gra&hs 1"6

*oncentration o +,$, .-0 Rate o Reaction cm' sec"1

( ()(% 3 ()183% 1( (),'4% 13 ()317% ,( ()68,% ,3 1),4%

'( ')(5% '3 ,)',%;ra&h 8


8/9

0 * +0 +* ,0 ,* 30 3* -00

0.*

+

+.*

,

,.*

3

3.*

Efect o the substrate concentration.+,$,0 on the rate o reaction

%oncentration of 8,",

ate of reaction cm6 sec9:

*onclusion

The data obtained from this experiment suggests that as the concentration of

hydrogen peroxide increases, the rate of reaction increases and that my

hypothesis is valid. The data shows that as the substrate concentration of

hydrogen peroxide increases the amount of oxygen production also increases; it

can be seen on the processed data where the average oxygen produced in *

concentration of hydrogen peroxide is *.3cm3 whereas in 30 concentration it is

//.0 cm

3

. "n the other hand,


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substrate increases the rate of reaction also increases. Aecond of all, the

uncertainty of 0.* is very low and cannot eect the reaction very much.

Third of all, the value was very reliable as when e?uals +.0, all points

lie exactly on a straight line or trend line. The values of in graph +B7 are

also very close to +.0 which suggests that the experiment was carriedpretty well and the results are also good. Cut, even though it was

performed carefully to minimize errors, in graph * there are only few

points on the trend line and the value is lower than the other graphs.

The highest value is in >raph 3 of 0.223/ which is very close to + and

the lowest value is in >raph * of 0./-+ which is ?uite far from + and

suggests that there were few errors.

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