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BPTI: Molecular Dynamics (300K)
A model of Bacteriorhodopsin
BR photocycle
t [ps]
C13 – C14
C14 – C15
13-14
14-15
NH
H N
Prot.
hν
W. Zinth et. al.
Time-resolved Pump-Probe Spectrum of Sensorhodopsin
W. Zinth et. al.
BR J
J K
hν
FTIR: Spectra from Interferograms
mirror position
IR spectrum of polystyrene
IR amide bands of proteins
Amide I band provides information on secondary structure
Monitoring protein unfolding by FTIR: folded
Monitoring protein unfolding by FTIR: unfolded
Difference FTIR
Fritz Siebert
Time resolved difference FTIR of bacteriorhodopsin
Fritz Siebert
rupture force
cantilever-position x
force
0
250 pN
Single Molecule Force Spectroscopy
E.-L. Florin, V. T. Moy, H. E. Gaub, Science 264, 415 (1994)
rupture force [pN]co
unts
Gautel et al., Science 1997, Biophysical Journal 1999Linke and Fernandez, J. of Muscle and Cell Mot. 2002
Force probe experiments (AFM, opt. tweezer):--> Ig domains as “shock absorbers“