13

BVisionBVision Dedicated for the Morris water maze test study of effect of different compounds on spatial orientation and memory of experimental animals

Embed Size (px)

Citation preview

Page 1: BVisionBVision Dedicated for the Morris water maze test study of effect of different compounds on spatial orientation and memory of experimental animals
Page 2: BVisionBVision Dedicated for the Morris water maze test study of effect of different compounds on spatial orientation and memory of experimental animals

BVisionDedicated for the

Morris water maze test

study of effect of different compounds on spatial orientation and

memory of experimental animals

Video camera

Water poolHidden Platform

Animal (rat)

BV

Page 3: BVisionBVision Dedicated for the Morris water maze test study of effect of different compounds on spatial orientation and memory of experimental animals

BVision

Data

Tracking System Analyzing Tools

Acquisition

Management

Visualization

Analysis

Configuration parameters

BV

Automatic creation of the video archive

Page 4: BVisionBVision Dedicated for the Morris water maze test study of effect of different compounds on spatial orientation and memory of experimental animals

BV

• Program correction of the video path in the framegrabber.

• Minimizing load of the processor.

• Fast signal proceeding (up to 25 frames/s

• Displaying zones boundaries

Live Video

Page 5: BVisionBVision Dedicated for the Morris water maze test study of effect of different compounds on spatial orientation and memory of experimental animals

Data structure and storage Flexible database management system.

Tracks and Experiments Stored in the Database

BV

Page 6: BVisionBVision Dedicated for the Morris water maze test study of effect of different compounds on spatial orientation and memory of experimental animals

Arena and zones definition Automatical detection of borders of the pool and other

analyzed zones (for example, platform)

Result of Zones Detection

Restoration of the Platform Position

BV

Page 7: BVisionBVision Dedicated for the Morris water maze test study of effect of different compounds on spatial orientation and memory of experimental animals

Object Detection Algorithm

Формальный нейрон

Framegrabber Input video signal

Program correction of the

video path

Differentiation of all the found

objects by size

Corrected video signal

Detected animal

1

Differentiation of the residual objects

according to physical reasons

2

BV

Page 8: BVisionBVision Dedicated for the Morris water maze test study of effect of different compounds on spatial orientation and memory of experimental animals

Tracking process

BV

Page 9: BVisionBVision Dedicated for the Morris water maze test study of effect of different compounds on spatial orientation and memory of experimental animals

Parameters

BV

• escape latency,

• accounting for time of active swimming only (with speed lower than selected threshold),

• trajectory length,

• speed,

• index of “pasive” swimming” (to characte-rize decrease of animals’ mobility),

• time in zone of interest (ZOI),

• % of tracks failed to find the platform,

• straightness index (deviation from the direct path to platform),

• cumulative and reverse cumulative distances to platform,

• angle characteristics of movement relative platform,

• Whishaw “alley” index, etc.

Page 10: BVisionBVision Dedicated for the Morris water maze test study of effect of different compounds on spatial orientation and memory of experimental animals

Analysis of a Single Track

BV

Page 11: BVisionBVision Dedicated for the Morris water maze test study of effect of different compounds on spatial orientation and memory of experimental animals

Zones Definitions for Analysis

BV

Page 12: BVisionBVision Dedicated for the Morris water maze test study of effect of different compounds on spatial orientation and memory of experimental animals

Main Analysis Window

BV

Page 13: BVisionBVision Dedicated for the Morris water maze test study of effect of different compounds on spatial orientation and memory of experimental animals

Results Visualization

BV