-
日本 家禽学会 誌
第 46巻 第 Jl 号 （2009年 4月）
目 次
研究報告
ニワト リ卵胞頼粒膜におけるプラスミノ ーゲン ・アクチベーター活性と小卵胞の急速成長相への転移との関係
…・・武石 勝 ・安住水穂 ・西田沙世・山村奈美子・ 後藤尚也・渋弁仁志 ・土井守 ・上吉道治 J l
PCR-RFLP法を用いた名古屋種雄の遅羽性遺伝子型判定
…...・H ・－…中村明弘 ・小林正直 ・野田賢治 ・近藤 ー ・神作宜男 J 9
乾燥酵母細胞壁 （バイオモス〉によるブロイラ一生産成績改善および排池物中へのサルモネラ排出抑制
…・・鈴木敏明・ 菊浦裕二 ・高知由紀 ・伊藤美雪 J 16
解説・情報・資料
ニワトリおよびウズラにおける遺伝育種学的研究の 100年....・H ・.....・H ・.....・H ・－－……・・都築政起
・後藤直樹 J 23
lT1T，』回音S団~jfJJll,!品店r..a …・ J 30 総説
アヒルのゲノム研究と応用
Y.H. Huang, N. Li, D.W. Burt and F. Wu
－……………・・…・・・・…・・W.P.S.J.64 (3) 329 341. 2008.
ガチョウにおける繁殖季節性の制御
Z.D. Shi, Y.B. Tian, W. Wu and Z.Y. Wang・・・・・…・－一－・…・・・・・H ・H
・－…W.P.S.よ64 (3) : 343-355. 2008.
ニワトリの受精卵の培養前後および初生雛における性判定法
E.F. Kaleta and T. Redmann －…・・・・・…・・・・….....・...…....・H
・........・・・・W.P.S.よ64(3) : 391 399. 2008.
9際化前の家禽匹における重要な代謝経路
J.E. De Oliveira, Z. Uni and P.R. Ferket ・ ・・・・H ・－－….....・H
・....・a・－－…W.P.S.J.64 (4) : 488-499. 2008.
ブロイラーの産肉能力と僻化体長や体重との関係
R. Molenaar, I.A.M. Reijrink, R. Meりerhofand H. Van Den
Brand
リ H ・H ・－…・・…・・…W.P.S.J.64 (4): 599 603. 2008.
ニワトリ店の非侵襲的心電図測定
F. Hab巴rmann,D. Feske and H. Tonhardt …－－….......・H ・....・H ・・・
・ W.P.S.J. 64 (4) : 605-610. 2008.
l'.';,fJ',~；司耐靖§＇－......：.•
2007年における遺伝子組換え作物の栽培状況 米持千里……....・H ・－－－・・H ・H ・...・H ・－－……H ・H
・...・H ・.....・H ・...J 37
1918年パンデミック（スペイン風邪）の原因ウイルスのもつ異常な増殖力と病原性は，
主にウイルス遺伝子の RNAポリメラーゼ複合体に起因する 小山卓美・佐藤国雄….........…....・ H ・・ J
39
2009年度春季大会演題 ・…....・H ・－－…・・……....・H ・....・H ・....・H ・－ －………・・・H
・H ・－…－…....・H ・－……….....・H ・..J 41
学会記事....・H
・－…......................................................................................................................•
・ J 44
-
Japanese Journal of Poultry Science
Vol. 46 No. Jl, April, 2009
Contents
Full Papers
Relationship between Plasminogen Activator Activity in Granulosa
and Follicular Transformation
to the Rapid Growth Phase of Small Follicles
・ Masaru Takeishi, Mizuho Azumi, Sayo Nishida, Namiko
Yamamura,
Hisaya Goto, Hitoshi Shibui, Osamu Doi and Michiharu Kamiyoshi J
1
Determination of Late Feathering Genotype in Nagoya Breed Males
Using PCR-RFLP Method
.. Akihiro Nakamura, Masanao Kobayashi Kenji Noda, Hajime Kondo
and Norio Kansaku J 9
Improved Productivity and Reduced Salmonella Shedding by a Dried
Yeast Cell Wall Product
(Bio-Mos) in Commercial Broiler Production
.. Toshiaki Suzuki, Yuji Kiku-ura, Tomoyuki Taka and Miyuki Ito
J 16
Commentary and Views
One Hundred Y巴arsof Breeding and Genetic Research in Domestic
Fowls and Japanese Quail
...・H ・－－…・MasaokiTsudzuki and Naoki Goto J 23
Japanese Abstracts of World’s Poultry Science Journal Papers
・・・・・・・・・・・・・・・・・・・・・・・・・・・・・口・・・・・・・・・・・ー・・・・・・・ J 30
News of Poultry Diseases, Eggs and Meats, Economics
…・…・・・…・…...・H ・.....・ H ・.....・H ・－－…...・ H ・－……・・ J 37
Paper Titles of 2009 JPSA Spring Meeting ・ .. ・………－ ...・H
・.....・ H ・.....・H ・...・H ・H ・H ・－・……・…・・・・…・・H ・H ・ J 41
Official Information of JPSA・H ・－－………...・ H ・.....・ H ・H ・H
・.....・H ・.....・H ・－－………・・・・…・・・…......・H ・.....・ H ・－．．， J 44
-
JPSAI, Japan Poultry Science Association
-=- r; t- 1) Q�§miJif.ll§it.:: to t:r � 7° 5 .7. � J - 1i' '/ ·
7 7 -J- � - Y -ft5it!t c
���0����ffi�0E3c®��
1 a,ls:1£-@;-WJFf1*:rt�t±rf:l:9
�tt� ;/1, -C, 1 tH 'o -c- i:. 1:, ,$:�!Re'
!iJJlfJI/jiffiff§--C-@;-ijx � n 7., PA
tJ1�,Jfil4X�ffi"'-O)fi;ftt:!ffl-g-T 7.i tJ,i§tJ,Hitt L t::o
;;;fs.:��c:/i, �::kN�ff§ (Fl) O)�j:?� 18 �Fs�M1;:ffi�-t 7.i
�JlJJ(;:, B§. v 1;··;1;- '/fffi;£N�¥.�iJ, G, ::k� � t;1 9 :ffi:§
0P"�ff?J
(F9) t;,i::, Fl ic:O)N�ff§'a:''!*l&Lt:: a
P"�ff§0!1:!iiillJJJi'1&, N�ff?Jlltcffil1Jlt:a:-�llilL,
N�fffilllt!.:::iolt7.i E2i/l0C'a:'7 :/�-1 £.. / 7 •:, -l?-1 c',
ffifj):Jlt/;::io IH, PA f.s'tt�§.*;!l!;'It� ffl \i' 7.,:;fjf!c:,
DNA�:/ 7 c,:. =-lv7 � :,,O)W2f!c:i![IJ;'E L. t: 0 � G
1;:, g1J0@{,t;:t;, GlaltfH.::'f*I& L t::N�ff§t;,
G*fl*1lx-!JJJc,�fl:4X L. N�ff?J*fl*1lx1:-W� L t:a ?�fffil!l!:S:ii
F6 t;, G F5 r;:iJ, 1:t-cff�t.:�1JD L. 'f:-01&1i 1i1l'[i1H,,s1
t.:�1JD L t:: a N�ff?J!J!ii±�1JDIH·N�fffil�jfil;ffi1:� L -c
�I±\ Lt::/jo/J'If;ftt/J$.(i, F6 t;, G F5 /;:tJ,if-C'ff�t.:�1JD
L, F5 t;, G F4 1.::tJ,1:t-c 1::·-1; �* Ltd&.
-c-O)'j&/iP"�ff?JO)��
/;:it£H�1>Lt::o PA f.s'tic E2 iilOCii. �/;: F8 iJ,G F7
KtJ>WCfJ21d�1JDL. F7 t.:::io,1-C�t>�Hl:a:-�Lt::f&, g�
ij§O)��/;:it£H�1> Lt.::o ffitj):JltO) DNA '@;';!i(i F9 t;, G
F5 /;:tJ, ,:t-c1;?iJ-?1;:�1JD Ltd&. t O)'j&1i
!ill'-Ji'--Ctltft Lt::o ffif1/ili*@fffil/j Ii F9 tJ, G F7 i c' (i
3-4 /eii c '&ic' JS -:o t::tJ:(, F6 --C fi 2�3 � c fJ. I') F5
/J, G Fl /;: :lo l 1 -C (i�/iic' as -:o t:a
L ;/1, G (i')*5�t;, G,
*�-�--C(it1'l?J�ff2JO)�,)filijx�ffi"'-O)fi;ft(i F6 C:�L -:J t::
cm�� n, f'Eft�jjijO) F7 r.:::io1i 1 -c PA $fie E2
�Jj'.t;t;!:\;1.:::J:�;/JD L, LtJ, t,� t, �Hl�� Lt: i:: ct;, G,
1]\?�/l?JO)�,Jfilijx�ffi"'-O)fi;ftt;: E2 O):IJ. tJ. G f', ffit1
Jlt/.:::io l;t 7., PA $''ti t,jffl-g-L -C,' 7., c �P� � ht.:: o
Jl
日本家禽学会誌， 46:Jl-JB, 2009
武石 勝1・安住水穂2・西田沙世2・山村奈美子2・後藤尚也1.
渋井仁志、1・土井守3.上吉道治2
キーワード ：プラスミノ ーゲン ・アクチベーター， 穎粒膜，エストラジオール 17，β，卵胞急速成長相，ニワトリ
緒雷
JPSA-PCテキストボックス
-
日 本家禽学会誌 46巻 Jl号（2009)
Relationship between Plasminogen Activator Activity in
Granulosa
and Follicular Transformation to the Rapid Growth
Phase of Small Follicles
Masaru Takeishi1, Mizuho Azumi2, Sayo Nishida2, Namiko
Yamamura2, Hisaya Goto1,
Hitoshi Shibui1, Osamu Doi3 and Michiharu Kamiyoshi2
1 Laboratory of Nippon Formula Feed Mfg. Co., Ltd. Tochigi
321-3621
2 Faculty of Agriculture, Gif University, Gif 501-1193
3 Faculty of Applied Biological Science, Gif University, Gif 501
1193
In domestic fowl, estradiol-17β （E2) is reported in relation to
the follicular transformation to the rapid
growth phase of small follicles. However, the pa此icipationof the
plasminogen activator (PA）加gran叫osa,which
is known to be involved in cell proliferation and extra-cellular
matrix remodeling, is not su血cientlyclear. To
confirm its participation, follicle喧fromthe largest follicle
(Fl) to the ninth largest follicle (F9) were collected at
18 hours before ovulation of the Fl and weighed. In the
follicles, the activity of PA in granulosa was m巴asured
using plasminogen extracted from rooster’s plasma and
chromogenic substrate. DNA content of granulosa was
determined by the diphenylamine method. Also, E2 in theca was
determined by radioimmunoassay. Morpholog-
ical investigation of ovarian follicles was implemented by the
usual method.
Follicular weight increased significantly from F6 to F5, and
thereafter in a straight manner until Fl. The
material transfer rate of follicles increased significantly from
F6 to F5, and the peak was from F5 to F4, and then
decreased according to the growth of follicles. Both the PA
activity of granulosa and the E2 concentration of
theca increased significantly from F8 to F7, showed a peak in F7
and decreased according to the growth of
follicles. The granulosa layer changed from multilayer into
monolayer in the developmental stage from F7 to F
5. DNA content of granulosa increased from F9 to F5, and
thereafter remained constant. In the present
experiment, follicular transformation of the rapid growth pha総
ofsmall follicles was found to occur in F6, and
both E2 and PA activity increased before the follicular
transformation. This suggests that not only E2 of theca
but also PA activity of granulosa participate in the
transformation to the rapid growth phase of small follicles.
(Japanese Journal of Poultry Science, 46: Jl-J8, 2009)
Key words ; chicken, estradiol-17.β，glanulosa layer, plasminogen
activatore activity, rapid growth phase
J8
-
JPSA� Japan Poultry Science Association
cpf1BJ:l5.l1 • iNiiE[�:2 · f:fEBiUt
1 • :ililii -1 • t$ff1r�2
1 �;J;O�!ll!�tl.g-�!���/!i'{luf'J'E��. �ffi��;J;Dfll:I�:� cf.UIT
'.fif'F 480-1193 2 Jf',fffi1':$l!ix�$J'.IB. t$�/ 11
!J¥J:ffltl/JRrniJH!!riZ2 229-8501
.:::...r; r IJ t3Jfiljjij9,( ;1,;;q=l3*Q)r"Jt£tt01 ;L,,;,
1-r:i][£1lcJIJi:tk:� U.:o -'c
-
中村ら：名古屋種雄の遅羽性遺伝子型判定
Determination of Late Feathering Genotype in Nagoya Breed
Males Using PCR-RFLP Method
Akihiro Nakamura1, Masanao Kobayashi2, Kenji Noda1,
H吋imeKondo1 and Norio Kansaku2
1 Animal Husbandry Research Division, Aichi-ken Agricultural
Research Center, Yazako, Nagakute, Aichi 480-1193, Japan
2 Laboratory of Animal Genetics and Breeding, Azabu University,
Fuchinobe, Sagamihara 229 8501, Japan
The avian endogenous virus gene (ev-21) is closely associated on
the dominant sex-linked late feathering (LF)
gene, K, on the Z chromosome of chickens. The LF and early
feathering (EF) phenotypes are widely used for sex
identi宣cation; when k+ /k+ sires are mated with Kl -hens, male
chicks are LF and females ar巴EF.To introduce
the feather sexing, two lines (EF male line and LF female line)
are m田 ssary.However, homozygous (K/K）卸d
heterozygous (Kンk+)late feathering genotypes exist泊 LFmales and
separating K/K and K/k + by phenotypic
differences at hatch is not possible. Consequently,
labor-intensive progeny testing has been required in order to
巴：stablishLF female line. In White Leghorn, determination of the
genotype can be conducted by using restriction
fragment length polymorphism (RFLP) analysis of the Kor k+ gene
fiat虫ingregion segments amplified by出e
polymeras巴 chainreaction (PCR). The present study was conducted
to establish a specific PCR assay that
distinguishes Nagoya breed K/K males from K/k+ males.
To investigate the differences of DNA sequence between Kand k+
genes in Nagoya breed, a total of 1456bp
of the flanking regions in K and k + was sequenced. Comparison
between K and k+ in Nagoya breed, indicated that
differences were detected at 7 positions. Transition was
detected at position 213, 294 and 616. Transversion was
detected at position 333, 694 and 794. Deletion was detected at
position 189-193 in K. At position 292-295, k+
contains a Mbo I site (GATC), whereas K did not contain the
recognition site. Thus, these results indicate that
the Mbo I RFLP can be used in Nagoya breed to differentiate the
Kand k+ alleles.
(Japanese Journal of Poultry Science, 46 : J9 JI 5, 2009)
Key words: feather sexing, Nagoya breed, polymeras巴
chainreaction, restriction fragment length polymorphism
Jl5
-
JPSA#, Japan Poultry Science Association
�1*M£HB3��m: C/"'-1' ;.t- -c 7-) t=ct. {) 7··0 -1' 5
-��nx*l��
S ct L}�Fi'lli:!W cp "'- 0) -tJ- J v -c -* 5 �F tf:H[p ftlj
1 !Jo/J@:/', 1 ::t -y- ·:1 J �A�H, *Ei:llB/il!IRz 105-0014 2
�A4H± � "'/�:;; 7 r - .b., Jffi!i2.���M-ffll::kilffli1HJ!f:i5
899-8313
3 ��JH�t±lli'ilw� ::1 o 1 '7 -,
f.'i'ilwb!/t�EBffll&.tl!flHJT/ill 708-0341
::k��i1Ei · -iJ- Jv-t: .t '7�0) :·:; .b.�ttlii-ooUIH,,
��i-ffiitf.51" ·HJ:J:l:JO)� 7->�1*M£HIB�� (r{1 :t -t: .:z.,
Alltech 1± �) O)�.g-1.:: J:. 7., ::to 1 :;
-O)�@'.tt&�w1,"=i-.=-::i0) ::to 1 :; -�@:lE�-n;i:� Lt::a
:iffi1/tO)lft'.��Mi-ffl �,-c-�O) ::to1 :;-i-�:@'.1"7.> Jt±t!i,
148�ll$309Jj':i.J;Ji-xi®U: Lt::o 33�ll$Hil:�1Rc: L, WJAAffl�*4
(5�14 Bffi(l) 1ue1 ;t 'E- 7. i- 0.3%, 0f&*"l 47 Bliff;tf:l?i/fi
"'t:' 0.1% O)m1JDi-fr� \ fflliff,;1J[]�*4�.!,j.O){fullf c:.l:tfx
Lt:: o fflli�O)l!ic�iii.1*'1-i-ffl, 1--C tMJIJ���i-�@:-t 7., 0 Htii
24 �'/l$15 ]J;J;Ji-t.t�,.::, fiJHt�M (0-10 Bliff;) · MAAmaiiJM
(11�24 Blil\i) O)lj./(1{,( ;t'E-.7. 0.2% i-m1JDL-C
6l�ll$1.::*�.g.l.,, ffuO) 18�'/l$i-ffl!im1JDIRc: L-c.tttx'.Lt::o
it::, Ot±tli-1J-1v-t:i:-70).1l!tj:IO) t=J�tllt!lltiJi-lJ. 7.,
t::10. {fuO) 12 l�M 12 n�i- 3 mi.:5ht, 1 UHttm1m!Rc: L, {-fuO) 2
m,t,{ 1 :ct 'E-.:z. i- 0.1 it:: tiD.2% i- 24 B8iU tm1JD Lt::a
i±l?i/f*"l 1 �rs,iJIJO)��Pl��1l!i-�7" t- ;,;7.1
-
日本家禽学会誌 46巻 Jl号（2009)
Improved Productivity and Reduced Salmonella Shedding by a
Dried Yeast Cell Wall Product (Bio四 Mos)in
Commercial Broiler Production
Toshiaki Suzuki1,・Yuji Kiku唱ura2,Tomoyuki Taka3 and Miyuki
Ito3
1 Bussan Biotech Co.,Ltd., Shiba 2屯homeDaimon building, 3-3,
Shiba 2-chome, Minato-ku, Tokyo, 105 0014
2 Japan Farm Co., Ltd., 3887 Nogata, Oosaki-cho, Soh-gun,
Kagosima”ken, 899-8313
3 Okayama-ken Broiler Co., Ltd., 1647-1 Simohara,
Kagam泊o-machi,Tomada-gun, Okayama-ken, 708-0341
A dried yeast cell wall product (Bio-Mos, Alltech Inc.), which
agglutinates gram-negative bacteria such as
coliforms and Salmonella and activates immunity, was tested on
two broiler companies for its effectiveness on
productivity. At Company J, which utilizes ordina:τy commercial
feeds, 148 flocks (3.09 million birds) were us巴d.
33 flocks were giv巴nBio
shipment. Other flocks were control birds without Bio-Mos. At
Company 0, which uses AGP free commercial
feeds, of 24 flocks (150,000 birds), 6 flocks w巴r巴given0.2%
Bio-Mos in both starter (0-10 day) and grower (11-
24 day), and the other 18 flocks were control birds without
Bio-Mos. To旬stSalmonella shedding, another
120,000 birds, 12 flocks, were allocated to three groups: one
control and two with Bio-Mos added at 0.1 or 0.2%
up to 24 days of age. Cecal droppings collected也 thehouses
around one week before shipping w巴reincubated in
peptone solution, Hajna tetrathionate broth and then on MLCB
medium. Bacterial numbers were counted by the
MPN method. Results showed that, at Company J, Bio-Mos improved
the commercialized rate during the three
months of th巴巴xperimentby 2.1%. At Company 0, the out grade rate
was reduced by approximately 50% and
the commercialized rate was improved by 2.5%. Salmonella numbers
in excre.ta was reduced in the Bio-Mos
叩 pplementedgroups, whereas high Salmonella shedding was
observed in the unsupplemented group. After 0.2%
。fBio-Mos were fed to all flocks up to 24 days of age,
Salmonella numbers during the next six months became lower than the
detection limit in all flocks, which strongly suggested that effect
of Bio-Mos remains even after its
withdrawal. Bio-Mos is considered to be effective for broiler
production and Salmonella control, regardless of
AGP usage.
(Japanese Journal of Poult.砂 Science,46: J16-J22, 2009)
Key words: Bio-Mos, broiler, commercialized rate, productivity,
Salmonella
