Catalogo Applied Stem Cell

Applied StemCell

Applied Stem Cell Inc.’s proprietary TARGATT™ technology enables highly efficient site-specific gene integration in mammalian cells and animals1, 2. This technology uses ФC31 integrase to insert any gene of interest into a docking site, pre-engineered in an intergenic region and transcriptionally active genomic locus. Our TARGATT™ technology improves several aspects in the generation of transgenic cell lines and animals: (1) High integration efficiency mediated by ФC31 integrase reduces time and cost; (2) Site-specific integration at a pre-selected genomic locus eliminates position effect and ensures high level expression of the transgene; (3) Integration at intergenic region ensures that no internal genes are interrupted; (4) Single copy gene integration eliminates repeat-induced gene silencing and genomic instability; (5) Site-specific integration allows a precise comparison of the effects of the transgenes among different lines.TARGATT™ technology can be utilized for a variety of applications including reporter gene expression, gene knockdown, disease cell and animal models.

Fast & Site-Specific Knock-in TechnologyFast & Site-Specific Knock-in Technology

Gene of interest (eg. GFP) TARGATT™ Vector with

Screening pups for site-specific gene integration

Transfection

Embryos with attp sites are collected from TARGATT™ mouse.

References:1. B. Tasic et al., Proc Natl Acad Sci USA. 108, 7902 (2011) .2. J. Rossant, L. M. J. Nutter, M. Gertsenstein, Proc Natl Acad Sci USA. 108, 7659

Background:

Applications Applications

How it works:

TARGATT™

attB

1

2

in vivo screenings Generation of humanized models Generation of disease modelsGeneration of drug / genome interaction models

in vitro screenings Drug discoveryStudy drug interactionsToxicity StudyPersonalized medicine

4

Toll Free Phone: 1-866-497-4180 www.appliedstemcell.com Applied StemCell Toll Free Phone: 1-866-497-4180 www.appliedstemcell.com

1 2

TARGATT™ Embryos & Transgenic Kit

Fast & Site-Specific Knock-in Mouse Service

TARGATT™ Services and Products

If you prefer to generate a knock-in mouse model on your own , TARGATT™ Embryo and Transgenic Kits are available for purchase.For your convenience, you may also mix and match any of our TARGATT™ products and services.

TARGATT™ H11 Embryos FVB (Frozen) AST-0002 $3,600* TARGATT™ H11 Embryos C57BL/6 (Frozen) AST-0012 $4,800* TARGATT™ Rosa26 Embryos FVB (Frozen) AST-0004 $3,600* TARGATT™ Transgenic Kit AST-1002 2 rounds of microinjections $750

MTA is required upon ordering. *Quotes for academic research. Please contact us for industrial rates.

Traditional Knock-in

Comparison of timelines for Knock-in Mouse Models

TARGATT™ System

Vector Design

Pronuclear Microinjection

F0 Genotyping

3 months

Vector Design

ES cellTargeting

Chimeras

Germline Mice

Blastocyst microinjection

Technology Overview

vs

10 months

Service Includes:Vector design and constructionIntegration enzyme generationDNA preparation Embryo microinjection / ImplantationAnimal care-housingGenotyping

Knock-in Mouse Service (C57BL/6 strain) AST-5021 $19,000 Knock-in Mouse Service (FVB strain) AST-5001 $17,000

Features:Fast: Our proprietary technology will generate your site-specific knock-in mouse in 3 months.Reliable: Any gene of interest can be specifically inserted at a defined, transcriptionally active locus. We guarantee transgene expression.Versatile: Tissue specific and/or ubiquitous expression are available.

Reliable Gene Expression

Efficiency Up to 40% Low Site-Specificity Yes Yes Transgene Expression Yes YesTransgene Copy Number One copy One copyTurnaround Time 3 Months 10 Months

TARGATT™ System

Traditional Rosa26 Knock-in

Generate master TARGATT™ Cells with attp sites (6 months)

TARGATT™ Cell Lines Expressing Gene of Interest

Knock-in Mouse Generation

in vivo Model in vitro Model

Stable Cell Line Generation

1

2

3

4

3Pronuclear injection into TARGATT™ embryos.

within 3 monthsKnock-in Mouse

within 1 - 3 monthsKnock-in Cell Line

Applied StemCell Toll Free Phone: 1-866-497-4180 www.appliedstemcell.com Applied StemCell Toll Free Phone: 1-866-497-4180 www.appliedstemcell.com

3 4

Cell Line Gene Modification Service

Based on our proprietary site-specific TARGATT™ technology for fast knock-in (KI) mouse generation, Applied StemCell (ASC) recently developed a TARGATT™ system specifically for introducing gene(s) of interest into mammalian cell lines. The unique advantage of this system is that any gene of interest can be inserted into a defined, transcriptionally-active locus with high gene expression in a single copy fashion.

Adding TARGATT™ sites to your cell line allows you to insert any gene of interest in a specific locus in the genome.

Site-Specific

Knockin efficiency

Transgene copy

Off-target event

Others

Yes

Very low

Single

Very Low

Yes

High

Single

Very Low

Yes

Low

Single

Yes

No

High

Multiple

Yes

No

High

Multiple

Yes

Technical Advantages of TARGATT™:

Application of the TARGATT™ System for the Generation of Site-Specific Knock-in Mammalian Cell Lines

TARGATT™ System

HomologousRecombination ZFN Viral SystemTransposon

HighExpression

Time Consuming

Costly &High mutation rates

Insertional Mutations

Biosafety Level 2

attB

Gene of Interest (eg. GFP)TARGATT™ Vector with

Neuron Other Cell TypesBlood Cell

Cells with attp site Cell Lines Expressing Gene of Interest Characterizaton

(RT-PCR, Staining)

Service Includes:Vector constructionTransfection / selection Cell screeningCell expansion / validationCell cryopreservation

Stem Cell Differentiation and Characterizaton

Master Cell Line Stable Cell Line Generation

3 months

ASC provides high quality, one stop services for the generation of gene targeted mouse models. Cost-effective: Applied StemCell, Inc. has years of experience and a high success rate in generating custom gene targeted mice using our proprietary germline transmittable mESC lines. High Quality: We have high QC standard checkpoints at every step to ensure your project progresses smoothly.Fast Turnaround Time: We streamline every step. Gene targeted mouse models can be generated in as fast as 7 months.

Conventional Knock-in/out Mouse Generation Service

DNA Cloning Services

Virus Packaging Service

Behavioral Phenotyping Service for Knock-in/out MiceCompare behavioral differences between transgenic mice & wild type mice.

We provide high titer to ultra-high titer, ready to use VSV-G pseudoviral packaging services.

Service Includes:Gene targeting vector constructionES cell targeting Cre/FLP recombination Chimera production via blastocyst injectionChimera breeding to germline Mouse genotyping

Chimera from blastocyst injection

Examples of services:Cloning of amplified DNA fragmentsGene targeting vector construction for homologous recombination Bacterial Artificial Chromosome (BAC) recombineeringGeneration of RNAi and inducible vectorsSite-specific mutagenesisDe novo gene synthesis

Other Mouse Models / Gene Targeting Services

Custom Knock-in Cell Line Service AST-6021 Inquire TARGATT™ Knock-in Cell Line Service AST-6001 $25,000

Knock-in/out Mouse Generation (C57BL/6 strain) ASC-5023 $30,000 Knock-in/out Mouse Generation (129 / hybrid) ASC-5003 $26,000

Gene Expression Vector ASC-7002 $3,000 - $6,000 Knock-in/out Vector ASC-7001 $6,000 - $9,000

Lentivirus Packaging Service Regular Titer >10^7 IFU/ml ASC-7011 $900 Lentivirus Packaging Service High Titer >10^8 IFU/ml ASC-7012 $1,100 Lentivirus Packaging Service Ultra-High Titer >10^9 IFU/ml ASC-7013 $1,400 Retrovirus Packaging Service Regular Titer >10^6 IFU/ml ASC-7021 $1,000 Retrovirus Packaging Service Regular Titer >10^7 IFU/ml ASC-7022 $1,200

Promo

Molecular Cloning Services


5 6

Mouse ESC Derivation Service ASC-6001 $2,900 Our scientists have successfully derived over 80 new mouse embryonic stem cell l ines, including those from gene targeted embryos.

To publish your new ES or iPS cell line, do you need:- Stringent molecular and functional assays to evaluate pluripotency criteria?- Karyotype analysis to rule out genetic aberrations?

We offer all services needed for a complete characterization of your pluripotent cell lines.

Teratoma Formation Analysis ServiceFeatures:- Turnaround time: 4- 12 weeks- Number of cells required / injection site: Mouse ESCs/iPSCs = 0.5 x 10^6 Human ESCs/iPSCs = 1 x 10^6 - Success Rate: >97%

Service Highlights:Injection Specifics: A total of 4 mice (3 experimental and 1 control) will be used. Each recipient mouse will be injected at two locations: in the kidney capsule and in the testis.Steps: Cell injection, teratoma harvesting, Tissue Sectioning, H&E staining and histology analysis of teratoma sections.

Service Includes:1. Complete report with histological analysis and high quality/publication grade images of endoderm, mesoderm and ectoderm formation. 2. Tissue blocks and H&E stained tissue section slides

Teratoma Formation Analysis (Human, Mouse) ASC-6008 $2,900

References:K. Chen et al., Stem Cell Research USA. 9, 237–248 (2012)We are the exclusive teratoma service provider for Coriell Institute (Patient Specific iPSCs). See our service reports here:http://ccr.coriell.org/Sections/Collections/NIGMS/ipsc_list.aspx?PgId=696&coll=GM

FAQQ: What is your success rate for teratoma formation?A: After analyzing control and test samples, we have determined that 90% of our injections give rise to verifiable teratomas. Injections into both the kidney capsule and the testis ensures teratoma formation from pluripotent cells in one or both organs. The site and degree of teratoma formation is cell line dependent. For example, only 82% of hIPSC form teratomas in both the kidney and the testis. Other cell lines form teratomas in only the kidney or testis. By injecting three mice at two sites per test sample, we ensure that the best conditions are used to observe teratoma formation.

Q: How long does it take to get results?A: To form mature teratomas, it takes 3-6 weeks for mouse ES/iPSCs and 5-10 weeks for hES/iPSCs. As a general practice, we allow the teratomas to grow for up to 12 weeks before we sacrifice the mice.

Q: How do I prepare cells for the assay?A: Follow the instructions outlined in the Requisition Form found at http://www.appliedstemcell.com/userfiles/files/Teratoma_Service_Requisition_Form.pdfTypically, two T75 flasks are sufficient for the assay. We recommend that the cells be shipped (by one day delivery) at 70% confluence in flasks filled completely with culture media.

Derivation and Characterization Services

Derivation Services

Derivation

Characterization Services

Immunocytochemistry (Mouse) ASC-6006 $599 Immunocytochemistry (Human) ASC-6007 $799 RT-PCR Inquire EB Formation Service (P.8) ASC-9020 $980 Teratoma Formation Analysis (P.6-7) ASC-6008 $2,900

Germline Transmission

Chromosome Counting ASC-6003-C $400 G-banding (Mouse) ASC-6003-GM $700 G-banding (Human) ASC-6003-GH $700 Detection of genetic abnormalitiesChromosome counting of metaphase spreads and G-banding Sex Determination ASC-6004 $200

Is it male or female? We will give you the answer!

Pluripotency Characterization

Karyotyping

Success Rate

iPSC Generation Service (P.9)

Key points

Type

ASC’s methods

mESC/miPSC

100% 100% 93.7% 25-40%Teratoma

Formation Rate

Turnaround Time

Cells needed 0.5-1 million/site 0.5-2 million/site 1-2 million/site 3-5 million/site

3-5 weeks 5-8 weeks 5-10 weeks 12-18 weeks

Di�erentiation distinctive distinctive distinctive poor

hESC hESChiPSC

Traditional methods


7

Enhanced differentiation capability:Our technique of injection under both the kidney capsule and testis results in distinct teratoma formations with differentiated tissues derived from all three germ layers (A to F).

High Teratoma Formation Rate:Three recipient mice per sample are dually injected under BOTH the kidney capsule and testis to ensure teratoma formation from pluripotent cells at one or both organs. ~82% of human iPS cell lines form teratomas in both the kidney and testis. 8.9% forms kidney tumor only, 2.6% forms testis tumor only.

Technique Highlights

Kidney

+ + 82.3 %

+ - 8.9 %- + 2.6 %

- - 6.2 %

Testis% of iPSC Lines Producing

Publication Grade Teratomas*

*Teratomas with distinct tissue types

Satisfaction Guaranteed!!Please contact us with the information

about your samples for details and pricing

A

D E F

G H I

B C

Embryoid Body (EB) Formation and Characterization Service

Features:- A complete service that includes embryoid body (EB) formation and characterization to assess the pluripotency of your ESC/iPSC lines.- A fast alternative to traditional teratoma formation methods. Our turnaround time is approximately 3 weeks.- Ideal for rapid initial screenings or large sample screenings.

EB Formation and Characterization Service ASC-9020 $980/sample (Additional $500 culture fee for frozen samples may apply)

A

B

I II

I. EB derived from GFP-expressing human ESCs.

II. β- Tubulin III (ecdoderm) SMA: Smooth muscle actin (mesoderm) AFP: Alpha fetal protein (endoderm)

β-Tubulin III SMA

AFP

Satisfaction Guaranteed!!Contact us with information


Service Includes:- Formation of EBs.- IHC staining of EBs for pluripotency markers (Alpha fetoprotein for endoderm, smooth muscle actin for mesodern and Beta III tubulin for ectoderm).- A full report that includes high resolution, publication-grade images.

8

A : Cartilage B : Neuronal rosetteC : GlandsD : Pigmentaed cellsE : Bone F : Stratified squamous epithelium G - I : Subcutaceous injections give rise to poorly differentiation tumor tissue.


9 10

Genome Footprint-Free iPS Cell Generation Service (mRNA/Episomal)Frustrated with the amount of time and effort required to generate and characterize iPS cell lines? Wishing your valuable time was directed towards addressing the high priority issues of your research project rather than the time consuming tasks of iPS cell line generation and characterization? Let Applied StemCell generate the iPS cell line for you.

Features:- Our non-integrating method ensures the absence of a genomic footprint: ideal for drug discovery and cell therapy applications.- Two methods are available: DNA episomal vectors containing the reprogramming factors or synthetic mRNA of each reprogramming factor.- A high reprogramming efficiency is achieved with both the mRNA (0.5%) and the episomal vectors (0.2%).- Both reprogramming vectors and mRNA are removed during the cell cycle. - Safety: both episomal vectors and mRNA are safe to handle.

Further Characterization Services Available:- Karyotyping- Embryoid Body (EB) Formation and Characterization to test iPSC pluripotency- Teratoma Formation Analysis Service to test iPSC pluripotency

iPSC Generation Service (mRNA) ASC-6022 $10,000 iPSC Generation Service (Episomal) ASC-6023 $10,000

Cell Recovery and QuarantinePrimary Cell Line

(i.e. skin fibroblasts)

ReprogrammingmRNA

or

Colony Formation

Colony Expansion

Cryopreservation

Transfection with Episomal DNA Vectors

or mRNA

Characterization viaantibody staining and

RT-PCRTRA-1-60

Oct-4

Reprogramming Plasmids

6-Step iPSC Generation Service can generate iPS cell lines using two proven genome footprint-free methods (mRNA or episomal vectors)

hESC/iPSC Neuronal Differentiaton Service Do you want to differentiate your pluripotent cell line into neurons or glial cells?Let us make your life easier! We will make Neural Stem Cells (NSCs) or Neural Crest Stem Cells (NCSCs) for you! -You send us your pluripotent cell line (or we can make one for you)-We use our proprietary neural induction protocol and reagents to differentiate your ESC/iPSC into neural stem cells (NSCs) or neural crest stem cells (NCSCs)-NSCs and NCSCs are proliferating cells that can be frozen and cultured over a prolonged period of time. They are also capable of further differentiation into multiple neural cell types.

NSCs Differentiation Service ASC-8001 $9,500 NCSCs Differentiation Service ASC-8001C $9,500

Related Services:Cardiomyocytes Differentiation Service ASC-8003 $5,000Skeltal Muscle Differentiation Service ASC-8005 $5,000

Neural Stem Cells (NSCs) / Neural Crest Stem Cells (NCSCs)Primary Cell Line

(i.e. Patient-Specific Skin Fibroblasts)

iPS Cells

Reprogramming

Direct Conversion

Left: NSC stained with Nestin (green) and Sox2 (red)

Oligodendrocytes

CardiomyocytesDopaminergic

Neurons

Skeletal MuscleHepatocytes

Differentiation

Characterization

Other Options

Normal & Patient-SpecificDisease Samples & iPSCsNow available online!

Cells / cDNA / Total RNA- Neurological Disorders- Oncogenic Disorders- Muscular Disorders- Genetic Disorders- Autoimmune Disorders and more.

Differentiation

Differentiation

Characterization

Satisfaction Guaranteed!Contact us with information


Generation and Differentiation Services


1112

References: *K. Takahashi et al., Cell. 131, 861-72 (2007)

ASC’s iPS Cell Generation Kit contains an optimized ratio of retroviral reprogramming vectors that has been modified for the consistent generation of iPS cells*. A user-friendly protocol along with our excellent customer service will make the task of iPS cell generation worry-free.

Complete iPS Cell Generation Kit ASK-3011 (up to 4 reactions) $1,200

Individual retrovirus is also available:Human iPSC VSVg Retrovirus Cocktail 0.4 mL ASR-2005 $1,000High Titer GFP VSVg Retrovirus 0.1 mL ASR-2006 $230

iPS Cell Generation Kit (Retrovirus)

Virus

GFP

hOCT4

hSOX2

DAPI Merge

Top row: BJ cells (Human Fibroblasts) were transduced for 24h with Retro-GFP (MOI: 0.2).

Kit Includes:1. A ready-to-use cocktail of retroviral reprogramming factors (Oct4, Sox2, Klf4 and LMyc)The concentration of each vector has been optimized to obtain a high reprogramming efficiency.2. Retro-GFP This GFP-containing vector is used for the determination of the optimal condition to maximize the transduction efficiency. 3. Polybrene To enhance efficiency of transduction.4. Complete medium Just mix the medium supplements and Basal hiPS cell medium included in the kit. 5. Feeder cells CF-1 MEFs are provided for candidate clone culturing

Features:High Reprogramming Efficiency User-Friendly ProtocolEvery batch of retrovirus is tested for its ability to generate iPSC colonies

ESC/iPSC Characterization Kit

ES/iPS Cell Pluripotency RT-PCR Kit

Features:Sophisticated Marker Selection: Each kit includes an optimal panel of pluripotency markers.

High Sensitivity: Partially differentiated hiPSC clones can be detected.

Convenient: The ready-to-use kit contains all the buffers and solutions needed to perform immunocytochemical and enzyme activity analyses.

Mouse ESC/iPSC Characterization Kit (20 reactions) ASK-3005 $200Human ESC/iPSC Characterization Kit (20 reactions) ASK-3006 $290

Easy-to-use RT-PCR kit to confirm the pluripotency of human/mouse ES/iPS cell lines with well established pluripotency markers.

Mouse ESC/iPSC Pluripotency RT-PCR Kit (20 reactions) ASK-6001 $50Human ESC/iPSC Pluripotency RT-PCR Kit (20 reactions) ASK-6101 $50

Hoechst staining Anti-SSEA-4 staining Merged

Human ES cell

Oct-4 Sox-2 SSEA-4 TRA-1-60 TRA-1-81 AP

+ +

+-

- -

-

+ - - - -

- -

- -

- -

-

+

+

+

+

+ +

+

+

Human Neural Stem Cell

Human Mesenchymal Stem Cell

Oral SCC9 β6 Cells

Human Amniotic Mesenchymal Stem Cell

Marker selection reference table:

Stem Cell Research Products

Kit Includes: 1. Easy-to-use primary and secondary antibodies with well-established pluripotency markers to confirm pluripotency of human/mouse ES/iPS cell lines. Primary antibodies Human: OCT4, SOX2, SSEA4, TRA-1-60, TRA-1-81 Mouse: OCT4, SOX2, SSEA12. Fixation, Permeabilization, Blocking, and Mounting Reagents3. DNA counterstain4. Alkaline Phosphatase activity assay

Best Selling


13 14

Neural Stem Cell Generation Kit

Looking for an alternative to expensive protein neural-induction reagents?Tired of the heterogeneous nature of embryoid body formation and the subsequent protracted length of neuronal differentiation?ASC’s Neural Stem Cell Generation Kit is your answer!

Neuro-Sure™ Medium: Number of Rosette Clusters / Well

Features:- Fast, efficient, and user-friendly- Special formula: Increases the efficiency of rosette formation by 10-fold over standard neuron and/or induction media- Generation of proliferating Neural Stem Cells (NSCs) that can give rise to large quantities of neuronal and glial cells- Suitable for the generation of various neural cells (glial cells, astrocytes, dopaminergic neurons, motor neurons)

ASK-4002 Neural Stem Cell Generation & Characterization Kit $700ASK-4003 Neural Stem Cell Generation Kit $500ASK-4001 Neuro-Sure™ Induction Medium Size: 100 mL $150ASK-4101 Neuro-Sure™ Culture Medium Size: 100 mL Inquire

Neuro-Sure™ (ASM-4001)Standard NIM

3 30

a) A single Rosette cluster is obeserved using standard neural induction medium.b) Multiple rosette clusters are observed using Neuro-Sure™ Medium.

Mouse Embryonic Fibroblast (MEF) cells are used as feeder layers for ES/iPS cell cultures. We have a broad selection of high-quality MEF products including:

CF-1 Neomycin-resistant DR4 (resistant to neomycin, hygromycin, puromycin, and 6- thioguanine)

DR4, P2, untreated ASF-1001 1 x 10^6 cells $180 DR4, P3, irradiated ASF-1013 4 x 10^6 cells $120 ASF-1015 2 x 10^6 cells $70

CF-1, P2, untreated ASF-1201 1 x 10^6 cells $90 CF-1, P3, irradiated ASF-1213 4 x 10^6 cells $45 ASF-1215 2 x 10^6 cells $35

CF-1, P3, mitomycin-C treated ASF-1223 4 x 10^6 cells $45 ASF-1225 2 x 10^6 cells $35

Neo-resistant, P2, untreated ASF-1101 1 x 10^6 cells $90 Neo-resistant, P3, irradiated ASF-1113 4 x 10^6 cells $45 ASF-1115 2 x 10^6 cells $35

SNLSNL 76/7 feeders, STO cell line, P12, untreated ASF-1305 2 x 10^6 cells $90 SNL 76/7 feeders, STO cell line, P14, irradiated ASF-1317 5 x 10^6 cells $45

SNL 76/71, 2,3, derived from the mouse fibroblast line STO, is transformed with both the neomycin and LIF genes and has been shown to support the growth of both ES and iPS cell lines.

References:1. Okita, K; Ichisaka, T; Yamanaka, S. Nature 448:313–317. (2007) 2.Takahashi K, Okita K, Nakagawa M, Yamanaka S. Nat Protoc. 2:3081-9. (2007) 3. Takahashi K, Narita M, Yokura M, Ichisaka T, Yamanaka S. PLoS One 4(12):e8067 (2009)

MEF Feeder Cells (Mouse Embryonic Fibroblasts)

- Average viability >95%- Tested for long term culture of either mouse or human ES/iPSC lines- Manufactured at our US facility- Rapid inventory turnover ensures fresh stocks are shipped within 2-3 days- Our MEFs have been used by more than 200 clients in both academic and industry labs.

CF-1


15 16

MEF Conditioned Medium ASC’s Conditioned Medium is harvested from cultured CF-1 MEFs. Our stringent quality control and functional control assays ensure the pluripotency of your ES/iPSC lines will be maintened in this feeder-free culture system.ASM-5008 100 ml $120

Serum- and Feeder-Free Medium (SFFM)ESC-Sure™ SFFM is an optimized medium formulated for the culture of human ES/iPSC lines requiring serum-free and feeder-free culture conditioins. ASM-5010 100 ml $99

Mouse ESC Culture Supplement ESC-Sure™ mESC Mate is a basal medium supplement that contains all the factors required by mESC lines.ASM-5011 100 ml $200

Mouse ESC Complete Medium ESC-Sure™ mESC Complete Medium is a ready-to-use medium that contains all the reagents required for mouse ESC and iPSC cultures. ASM-5022 500ml $180

Neural Stem Cell Differentiation MediumNeuro-Sure Differentiation Medium is a specially formulated medium designed to increase the efficiency of the differentiation of ESCs or iPSCs into neural epithelia. The increase in differentiation efficiency is 10-fold higher than those resulting from standard neural induction media formulas. ASM-4011 100ml $120　

ESC/iPSC Culture Media

Stem Cell Growth / Differentiation Factors Wnt PathwayASG-2003-1 DKK-1 (Human Cells) 25 ug $240ASG-2005-1 Frizzled-5 Fc Fusion (Human Cells) 50 ug $210ASG-2006-1 Frizzled-8 Fc Fusion (Human Cells) 50 ug $210ASG-2011-1 SFRP5 (E.Coli) 50 ug $330ASG-2014-1 Wnt-3a (Human Cells) 5 ug $280ASG-2015-1 Wnt-5a (Human Cells) 5 ug $380

Notch PathwayASI-0030-1 DAPT 10 mg $180ASG-2009-1 JAG-1 (Synthesized) 100 ug $110

Hedgehog PathwayASG-2012-1 Sonic Hedgehog (Human cells) 5 ug $260

TGF-beta Family PathwayASG-2001-1 Activin A (Human Cells) 5 ug $180ASG-2002-1 BMP-4 (Human Cells) 5 ug $195 ASG-1012-1 BMP-7 (Human Cells) 10 ug $213ASG-2010-1 Noggin (Human Cells) 50 ug $215ASG-2013-1 TGF-beta1 (Human Cells) 5 ug $199

OthersASG-0003 bFGF (E.Coli) 50 ug $95ASG-1010-1 beta-NGF (Human Cells) 10 ug $104ASG-2004-1 EGF (E .Coli) 50 ug $85ASG-1014-1 EPO (Human Cells) 10 ug $240ASG-1004-3 GM-CSF (Human Cells) 10 ug $213 ASG-1006-3 IFN-alpha 2A (Human Cells) 10 ug $142ASG-2007-1 IGF-I Long R3 (E. Coli) 100 ug $120ASG-2008-1 Insulin (E. Coli) 1000 ug $110ASG-1005-2 IL-2 (Human Cells 10 ug $109ASG-1007-2 M-CSF (Human Cells) 10 ug $207ASG-1009-2 TNF-alpha (Human Cells) 10 ug $87

Request samples!We provide free samples of bFGF, Wnt-3a, TGF-beta1, Noggin, and BMP-4.Contact us at [email protected]. Promo # ASCRP08

GermLine* C57BL/6-EZ ASE-9007 2.5 x 10^6 cells $900 GermLine* C57BL/129SvJ ASE-9005 1 x 10^6 cells $795 GermLine* 129-EZ ASE-9006 2.5 x 10^6 cells $795 GermLine* mESC BALB/c EZ ASE-9008 2.5 x 10^6 cells $600 mESC C57BL/6-ALBINO ASE-9009 1 x 10^6 cells $795

*Germline transmission guaranteed

Human iPS Cell Lines generated by retrovirus vectors or episomal expression.

Human iPS Cells (Retro) ASE-9101 2x10^5 cells/vial $850Human iPS Cells (Episomal) ASE-9102 2x10^5 cells/vial $1200

ESC-Sure™ Fetal Bovine Serum (FBS) is the only serum product on the market that is tested for germline transmission of mouse ES cells.

Germline transmission tested! Mycoplasma-free Ready-to-use (no heat inactivation needed) Virus-free Toxin-free Competitively priced Each lot of ESC-Sure™ FBS is put through a series of quality control tests in addition to a series of functional tests. These tests ensure that each lot of ESC-Sure™ supports the normal morphology, karyotype, pluripotency, and germline transmission of mouse ESCs. Moreover, the series of quality control tests are conducted over several freeze/thaw cycles, therefore ensuring that every lot of ESC-Sure™ purchased is of the highest quality.

ESC-Sure™ Fetal Bovine serum, Germline transmission tested ASM-5006 100 ml $120 ESC-Sure™ Fetal Bovine serum, Germline transmission tested ASM-5007 500 ml $420

Germline Transmission Tested ESC-Sure™ Fetal Bovine Serum (FBS)

Mouse ES cells cultured in ESC-Sure™ FBS maintain normal morphology and pluripotency. ES cells cultured in our FBS are competent for tetraploid complementation.

Germline transmission test by tetraploid complementation

Mouse derived from injected ES cells (agouti)

Mouse ES cells cultured in ESC-Sure™ FBS

Mouse ES Cell Lines

Human iPS Cell Lines


17

MEF Feeder Cells

ASF-1001 DR4 MEF, P2, untreated 1 vial of 1x10^6 cells $180

ASF-1002 DR4 MEF, P2, untreated 3 vials of 1x10^6 cells $490

ASF-1013 DR4 MEF, P3, irradiated 1 vial of 4x10^6 cells $120

ASF-1014 DR4 MEF, P3, irradiated 5 vials of 4x10^6 cells $550

ASF-1015 DR4 MEF, P3, irradiated 1 vial of 2x10^6 cells $70

ASF-1016 DR4 MEF, P3, irradiated 5 vials of 2x10^6 cells $325

ASF-1101 Neo-resistant MEF, P2, untreated 1 vial of 1x10^6 cells $90

ASF-1102 Neo-resistant MEF, P2, untreated 3 vials of 1x10^6 cells $255

ASF-1113 Neo-resistant MEF, P3, irradiated 1 vial of 4x10^6 cells $45

ASF-1114 Neo-resistant MEF, P3, irradiated 5 vials of 4x10^6 cells $215

ASF-1115 Neo-resistant MEF, P3, irradiated 1 vial of 2x10^6 cells $35

ASF-1116 Neo-resistant MEF, P3, irradiated 8 vials of 2x10^6 cells $256

ASF-1201 CF-1 MEF, P2, untreated 1 vial of 1x10^6 cells $90

ASF-1202 CF-1 MEF, P2, untreated 3 vials of 1x10^6 cells $255

ASF-1213 CF-1 MEF, P3, irradiated 1 vial of 4x10^6 cells $45

ASF-1214 CF-1 MEF, P3, irradiated 5 vials of 4x10^6 cells $215


ASF-1216 CF-1 MEF, P3, irradiated 8 vials of 2x10^6 cells $256


ASF-1223 CF-1 MEF, P3, mytomycin-C treated 1 vial of 4x10^6 cells $45

ASF-1224 CF-1 MEF, P3, mytomycin-C treated 5 vials of 4x10^6 cells $215

ASF-1225 CF-1 MEF, P3, mytomycin-C treated 1 vial of 2x10^6 cells $35

ASF-1226 CF-1 MEF, P3, mytomycin-C treated 8 vials of 2x10^6 cells $256

ASF-1305 SNL 76/7 cell line, P12, untreated 1 vial of 2x10^6 cells $90

ASF-1317 SNL 76/7 cell line, P14, irradiated 1 vial of 5x10^6 cells $45

ASF-1318 SNL 76/7 cell line, P14, irradiated 5 vials of 5x10^6 cells $215

Media / Serum

ASM-4001 Neuro-Sure™ Medium 100 mL $150

ASM-5001 ESC-Sure™ Mouse DMEM 10 bottles of 450 mL (Set) $180

ASM-5002 ESC-Sure™ Human DMEM/F12 10 bottles of 400 mL (Set) $210

ASM-5003 Mouse ES Cell Freezing Medium 50 mL $65

ASM-5004 Human ES Cell Freezing Medium 50 mL $68

ASM-5006 ESC-Sure™ FBS, germline-transmission tested 100 mL $120

ASM-5007 ESC-Sure™ FBS, germline-transmission tested 500 mL $420

ASM-5008 ESC-Sure™ Conditioned Medium for hESC/iPSC 100 mL $120

ASM-5010 Serum-/Feeder-free Medium for hESC/iPSC 100 mL $99

ASM-5011 ESC-Sure™ mESC Mate 100 mL $200

ASM-5012 ESC-Sure™ Embryoid Body Formation Medium 100 mL $40

ASM-5013 ESC-Sure™ Embryoid Body Formation Medium 500 mL $170

ASM-5016 ESC-Sure™ ES Grade FBS 100 mL $95

ASM-5017 ESC-Sure™ ES Grade FBS 500 mL $320

ASM-5021 mESC Complete Medium 100 mL $60

ASM-5022 mESC Complete Medium 500 mL $180

Cell Culture Plates

AM-ART201 NAMEX™ HSC Expansion Kit (6-well) 1 kit $1349

AM-ART202 NAMEX™ HSC Expansion Kit (24-well)1 kit $1399

Mouse ES Cell Lines / Human iPS Cells

ASE-9005 GermLine mESC C57BL6/129SvJ 1x10^6 cells $795

ASE-9006 GermLine mESC 129-EZ 2.5x10^6 cells $795

ASE-9007 GermLine mESC C57BL/6-EZ 2.5x10^6 cells $900

ASE-9008 GermLine mESC BALB/c EZ 2.5x10^6 cells $600

ASE-9009 mESC C57BL/6-ALBINO 1x10^6 cells $795

ASE-9101 iPS Cell Line (Retrovirus) 2x10^5 cells $850

ASE-9102 iPS Cell Line (Episomal) 2x10^5 cells $1200

Antibodies and Characterization Kit

ASA-0001 Goat-α-mouse, 590 nm excitation 2 mL $20

ASA-0003 Goat-α-rabbit, 590 nm excitation 2 mL $20

ASA-0004 Goat-α-mouse, 488 nm excitation 2 mL $20

ASA-0006 Goat-α-rabbit, 488 nm excitation 2 mL $20

ASA-0110 α-Oct4 (h/m), monoclonal 800 uL $80

ASA-0120 α-Sox2 (h/m), monoclonal 800 uL $80

ASA-0135 α-SSEA-1 (m), monoclonal 800 uL $80

ASA-0150 α-SSEA-4 (m), monoclonal 800 uL $80

ASA-0160 α-TRA-1-60 (h), monoclonal 800 uL $80

ASA-0170 α-TRA-1-81 (h), monoclonal 800 uL $80

ASA-1011 Anti-WNT-3a (h/m), polyclonal 100 ug $260

ASA-1012 Anti-WNT-5a (h/m), polyclonal 100 ug $260

ASA-4010 Anti-Nestin (h), monoclonal 800 uL $80

ASK-3005 Mouse ESC/iPSC Characterization Kit 1 kit (20 reactions) $399

ASK-3006 Human ESC/iPSC Characterization Kit 1 kit (20 rxn) $579

ASB-0001 Alkaline Phosphatase (AP) Test Solution 1.5 mL $30

ASB-0101 Cell Fixation Solution 15 mL $20

ASB-0102 Permeabilization Solution 15 mL $20

ASB-0103 Blocking Solution 15 mL $30

ASB-0104 DNA Staining Solution 5 mL $20

ASB-0105 Anti-Quench Mounting Solution 5 mL $20

Reprogramming and Differentiation

ASR-2005 Human iPSC VSVg Retrovirus Cocktail 0.4 mL $1000

ASR-2006 High Titer GFP VSVg Retrovirus 0.1 mL $230

ASK-3011 iPS Cell Generation Kit 1 kit (4 reactions) $1200

ASK-4002 NSC Generation & Characterization Kit 1 kit (5 rxn) $700

ASK-4003 NSC Generation Kit 1 kit (5 reactions) $500

ASKHZ-0001 G4 Dendritic Cell Generation Kit 100 mL $299

ASKHZ-0002 G4 Dendritic Cell Generation Kit 500 mL $1299

TARGATT™ Series

AST-0001 TARGATT™ H11 Fresh Embryos (FVB) 25-35 embryos Inquire

AST-0002 TARGATT™ H11 Frozen Embryos (FVB) 45-50 embryos Inquire

AST-0011 TARGATT™ H11 Fresh Embryos (B6) 25-35 embryos Inquire

AST-0012 TARGATT™ H11 Frozen Embryos (B6) 45-50 embryos Inquire

AST-0003 TARGATT™ Rosa26 Fresh Embryos (FVB) 25-35 embryos Inquire

AST-0004 TARGATT™ Rosa 26 Frozen Embryos (FVB)45-50 embryos Inquire

AST-1001 TARGATT™ Transgenic Kit (5 microinjections) $1450

AST-1002 TARGATT™ Transgenic Kit (2 microinjections) $750

AST-2001 TARGATT™ H11 Mouse Tail Lysis PCR Kit 100 rxn $189

AST-2002 TARGATT™ Rosa26 Mouse Tail Lysis PCR Kit 100 rxn $189

AST-3001 to AST-3017: TARGATT™ plasmids (2 ug). Available upon request.

Assays and Arrays

ASCAP-0007 Stem Cell miRNA Array 3 reactions $519

ASCMA-0129 Stem Cell-Specific miRNA Plate Assay Kit I 12 rxns $449

ASCMA-0130 Stem Cell-Specific miRNA Plate Assay Kit II 12 rxns $449

ASCMA-0102 Stem Cell-Specific miRNA Plate Assay Kit III 12 rxns $449

ASCMA-0131 Stem Cell-Specific miRNA Plate Assay Kit IV 12 rxns $449

ASCMA-1003 Stem Cell-Associated miRNA Plate Assay Kit 2 rxns $409

ASCFA-1003 Stem Cell TF Activation Profiling Plate Array I 6 rxns $599

ASCAP-0171 Wnt/b-Catenin Regulated cDNA Plate Array (h) 4 rxns $409

ASCAP-0181 Stem Cell Marker cDNA Plate Array (h) 3 rxns $409

ASCAP-1171 Wnt/b-Catenin Regulated cDNA Plate Array (m) 4 rxns $409

ASCSC-0001 Single Stem Cell Gene Expression Assay Kit 12 rxns $469

ASCSK-0001 Nuclear Extraction Kit 1 kit (200 rxns) $99

ASK-6001 mESC/iPSC Pluripotency RT-PCR Kit 1 kit (20 rxns) $50

ASK-6101 hESC/iPSC Pluripotency RT-PCR Kit 1 kit (20 rxns) $50

Growth Factors

ASG-0003 Recombinant Human bFGF 50 ug $95

ASG-2004-1 Recombinant Human EGF 50 ug $85



ASG-2007-1 Recombinant Human IGF-I Long R3 100 ug $120

ASG-2008-1 Recombinant Human Insulin 1000 ug $110

ASG-2011-1 Recombinant Human SFRP5 50 ug $330

ASG-2001-1 Recombinant Human/Mouse Activin A 5 ug $180

ASG-2001-2 Recombinant Human/Mouse Activin A 25 ug $750

ASG-2002-1 Recombinant Human BMP-4 5 ug $195

ASG-2002-2 Recombinant Human BMP-4 25 ug $960

ASG-2003-1 Recombinant Human DKK-1 25 ug $240

ASG-2003-2 Recombinant Human DKK-1 100 ug $980

ASG-2005-1 Recombinant Frizzled-5 Fc Fusion 50 ug $210




ASG-2010-1 Recombinant Human Noggin 50 ug $215

ASG-2010-2 Recombinant Human Noggin 250 ug $920

ASG-2012-1 Recombinant Human Sonic Hedgehog 5 ug $260

ASG-2013-1 Recombinant Human TGF-beta 1 5 ug $199

ASG-2014-1 Recombinant Human WNT-3a 5 ug $280

ASG-2015-1 Recombinant Human WNT-5a 5 ug $380

Small Molecules

ASG-2009-1 JAG-1 protein active peptide fragment 100 ug $110

18

Documents

Catalogo Applied Stem Cell