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Introduction:
Established as Department of Bacteriology & Hygiene in 1965, the department has taken consistent lead role for
teaching and research activities in the disciplines of microbiology and immunology. The faculty is trained in modern methods of teaching and research, and keeps updated in knowledge and advances in these disciplines. The
department has been consistently making tangible contributions towards prevention and control of infectious
animal diseases. The pioneering and first-time lead studies have been made in the fields of poxviruses, salmonellae and other enterobacteria, foot-and-mouth disease virus, blue-tongue virus, equine herpesvirus, bovine rotaviruses,
buffalo immunology, experimental vaccines, genetically engineered nanobody production by phage display
technology, monoclonal antibody-based and molecular diagnostic tests, etc. The faculty and students have won several awards, honours and recognition, including ICAR Team Award, Rafi Ahmad Kidwai Memorial Prize, Hari
Om Trust Award, National Fellows and Professor, Fellows of the national societies, post-doctoral fellowships,
Nehru Award for best PhD thesis, etc. for their contributions in the disciplines of microbiology and immunology.
Since c. 1995, the department as ICAR Centre for Advanced Studies/Faculty Training has trained in advances in microbiology & immunology faculty from various SAUs and ICAR institutions in India.
Objectives of CAFT:
1. To impart training(s) on advances in veterinary microbiology & immunology to the
faculty/scientists in SAUs and ICAR institutions in India
2. To develop advance teaching and research methods & tools in the disciplines of
microbiology and immunology for strengthening education
Faculty: Name & Designation (as on 31.12.2019):
Sr.
No.
Name and Qualification Designation
1 Dr. Naresh Kumar Kakker, B.V.Sc. & A.H., M.V.Sc., Ph.D.
(UK), PDF (UK) Pr. Scientist &
HoD
Director, ICAR-
CAFT
2 Dr. (Mrs) Archana Sharma, B.V.Sc. & A.H., M.V.Sc., Ph.D.
(India) Professor
3 Dr. Jagveer Rawat, B.V.Sc. & A.H., M.V.Sc., Ph.D. (UK)
Associate
Professor
4 Dr Swati Dahiya, B.V.Sc. & A.H., M.V.Sc., Ph.D.
(India)
Scientist
5 Dr Rajesh Chhabra. B.V.Sc. & A.H., M.V.Sc., Ph.D.
(UK)
Scientist
6 Dr. (Mrs.) Vandana Bhanot, B.V.Sc. & A.H., M.V.Sc., Ph.D. (India)
ADIO
CENTRE OF ADVANCED FACULTY TRAINING
IN VETERINARY MICROBIOLOGY
DEPARTMENT OF VETERINARY MICROBIOLOGY
LALA LAJPAT RAI UNIVERSITY OF VETERINARY & ANIMAL SCIENCES
HISAR, HARYANA
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7 Dr. P. S. Cheema, B.V.Sc. & A.H., M.V.Sc., Ph.D.
(India)
ADIO
8 Dr. Pankaj Kumar, B.V.Sc. & A.H., M.V.Sc., Ph.D.
(India)
ADIO
9 Dr. Anshul Lather B.V.Sc. & A.H., M.V.Sc., Ph.D.
(India)
Assistant Professor
10 Dr. Akhil Kumar B.V.Sc. & A.H., M.V.Sc., Ph.D.
(India)
Scientist
11 Dr. Mahavir Singh B.V.Sc. & A.H., M.V.Sc., Ph.D.
(India)
Scientist
12 Dr. Parveen Kumar B.V.Sc. & A.H., M.V.Sc.,
Ph.D. (India)
Assistant
Professor
13 Dr. Anita Dalal B.V.Sc. & A.H., M.V.Sc., Ph.D.
(India)
Assistant
Professor
14 Dr. Sanjeevna Kumari B.V.Sc. & A.H., M.V.Sc.,
Ph.D. (India)
Assistant
Professor
15 Dr. Rahul Yadav B.V.Sc. & A.H., M.V.Sc., Ph.D.
(India)
Scientist
16 Dr. Ajit Singh, B.V.Sc. & A.H., M.V.Sc., Ph.D.
(India), PDF (UK)
ICAR-
Emeritus
Scientist
Human Resource Development:
Particulars of Short Courses/Training program for HRD conducted during 2017-20
S. No. Name of the Program Period No. of Participants
Internal External Total
1. Modern Technologies for
Production and Applications
of Antibodies for Animal Health Improvement
March 1 to 21, 2017 (3
weeks)
2 23 25
2. Microbial Genomics and
Proteomics in Diagnosis and
Control of Diseases of Veterinary Importance
November 7 to 27,
2017 (3weeks)
2 13 15
3. Diagnosis and Control of
Emerging and Re-emerging
Trans-boundary Diseases of
Poultry
February 7 to 27,
2018 (3
weeks)
2 23 25
4. Recent Advances in Cell
based Technologies for
Animal Disease Diagnostics
and Therapeutic
October 11 to 31,
2018 (3 weeks)
3 11 14
5. Microbiological Techniques for Diagnosis of Emerging
and Re-emerging diseases of
Veterinary Importance
November 20 to
December 10, 2019 (3 weeks)
4 11 15
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Infrastructure development (equipments, etc.):
Equipments associated with Department of Veterinary Microbiology
Sr.No. Name of equipment/article
Quantity
A. FOR RESEARCH
1 Pipette washer One
2 MicroPipette controller Three
3 Electronic Balance One
4 Computer Operator Chair with Arms One
5 Water Distillation 10ltr. Cap. One
6 Finnpipette India 0.5-10µl Four
7 Finnpipette India 40-200µl Four
8 Finnpipette Digital 30-300µl 8Channel Four
9 Extension Board Four
10 Spin coProjection Microscope One
11 Hot Air Oven 915x610x610mm One
12 BOD Incubator (SEW) One
13 Pipette Stand Four
14 Low Temp. Res. Cabinet–20⁰C One
15 Laminar Flow 4’x2’x2’ Two
16 Voltage stabilizer 5KVA Four
17 Voltage stabilizer 1KVA One
18 Autoclave 600x450mm One
19 Refrigerator Kelvinator 310 One
20 Refrigerator Voltas Two
21 Enameled Tray 12”*15” Six
22 Enameled Tray 10”*12” Six
23 Carboys 30Ltr capacity Two
24 Carboys 20Ltr capacity Four
25 Tray Plastic 24”x16”x15” Four
26 UPS1000VA with Battery One
27 BOD 375x570x550 NSW Two
28 Getner Biolux Research Binocular Microscope Two
29 Vaccum Pressure Pump Two
30 Filco Digital pH Meter One
31 Servo Voltage Stabilizer 5KVA Two
32 Servo Voltage Stabilizer 4KVA One
33 Weswox inverted Tissue culture Trinocular Microscope One
34 Laminar Flow4’x2’x2’ Two
35 Vortex Mixer One
36 Compact Laboratory Centrifuge One
37 Electrophoresis Power Supply One
38 Vertical Gel regular Dual One
39 Submarine Analytical Model One
40 Leveling Table25cmX15cm One
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41 Glass Plates Four sets
42 Acrylic Clamp Screws(2) One set
43 Gel Casting unit One
44 Table Top centrifuge One
45 Digital Electronic Top Loading Balance MAC-22 One
46 Deep Frezer(Remi), 0-20⁰C One
47 Steel Almirah(Big) Six
48 Steel Almirah(Small) Four
49 Serological Water Bath One
50 Air Coolers Five
51 Air Conditioners Three
52 Remi Magnetic Stirrer One
53 Vertical Autoclave 355x550mm One
54 Thermocyclers Three
55 CO2 incubators Two
56 IR concentrator One
57 MilliQ water purification system One
58 Vertical & horizontal electrophoresis system with power supplies Two
59 Western blot system One
60 Shaker incubator with cooling One
61 Centrifuge, highspeed, cooling One
62 Microcentrifuge, highspeed, cooling One
63 Biosafety cabinets Two
64 UV/vistransilluminator, with digital imaging Two
65 Cryocans, liquid nitrogen cylinders Four
66 Micropipetting devices Twenty
67 Cryopreservation facilities, -70°C Two
68 Ultrasonicator One
69 ELISA reader Two
70 Nucleic acid & protein quantification system One
71 Alpha Imager 1220 Gel Documentation and Analysis
System with Accessories One
72 Carl Zeiss Trinocular Inverted Microscope One
73 Thermal Cycler (transferred from BPD) One
74 ELISA Reader (transferred from BPD) One
75 Biological safety Cabinet (BSC) Class II, Type A2 One
76 Gel Documentation system One
77 96-Well Gradient Thermal Cycler One
78 Refrigerated Microcentrifuge One
B. For Teaching
1 Silver coated Projection Screen wall and mounting Two
2 Portable 0HP One
3 Automatic Slide Projector One
4 Episcope Model EA-2A One
5 LCD Projector One
6 Photocopier machine One
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7 Computer Work Station One
8 Computer Three
9 HPLaserjet 6P One
10 Modem One
11 Spectrophotometer One
12 Colony Counter One
13 Hand Held UV Lamp One
14 Compound Microscope Twenty Five
15 Refrigerator Two
16 Serological Water Bath One
17 Egg Incubator One
18 Refrigerated Centrifuge One
Technologies, products & processes developed:
1. Monoclonal antibody production by hybridoma technology
2. Monoclonal antibody-based ELISA kit for estimation of immune status of cattle and buffaloes
vaccinated against H.S.
3. Monoclonal antibody-based latex agglutination test (called TE-LAT) for detection of Trypanosoma
evansi antigen (‘surra’ infection) in animals (In collaboration with Regional Research
Station,Uchani).
4. Phage display technology for production of recombinant single-domain antibody of Indian desert
camel.
5. Phage display library of single-domain antibodies (>107 clones) of E. coli
lipopolysachharide- immunized Indian desert camel.
6. E.coli LPS-binding single-domain antibody clones selected from the phage display library
mentioned above & deposited in VTCC, NRCE, Hisar.
7. Staphylococcus aureus β-hemolysin-neutralizing single-domain antibody clone selected from the
phage display library mentioned above & deposited in VTCC, NRCE, Hisar.
8. DIVA test (3A-NSP antigen based ELISA) as per OIE protocol in collaboration with Freidrich Loeffler
Institute, Germany for differentiation of FMD infected and vaccinated animals.
Services offered to farmers/generalpublic:
1. PCR-based confirmatory diagnostic test for H.S.
2. Latex agglutination test for diagnosis of Trypanosoma evansi infection in domestic animals (In
collaboration with Department of Veterinary Parasitology).
3. PCR–based diagnostic tests for hemoprotozoa infections (In collaboration with Department of
Veterinary Parasitology).
Renovation of Lecture Room/Laboratories:
1. Lecture room renovated and equipped with LCD & overhead projector. 2. Seminar/Conference room renovated and equipped with LCD projector.
3. Renovation of the office of Head-cum-Director.
4. A well-maintained laboratory for CAFT trainings, headed by a professor in the faculty.
Page 6
5. UG laboratory renovated, white board and microbiology/immunology display boards fixed.
6. Immunotechnology laboratory established for work on production of monoclonal antibodies and phage display technology for nanobody production.
7. RRC on FMD virus is housed in an air-conditioned building, equipped with all modern facilities.
8. Hemorrhagic septicaemia laboratory is renovated with air-conditioning & modern equipment.
9. Media & cleaning room renovated.
Library upgraded:
A. Library room renovated: Air-conditioned room having all collections of books, theses,
manuals, proceedings of summer institutes, etc.
B. Computers with Internet facilities to all faculty members.
C. CeRa access to journals & books from leading publishers via Nehru Library, CCSHAU,
Hisar.
D. List of books added to the departmental library:
Sr.
No.
Title of the Book Authors/Editors Year of
Publicatio
n
1. Cell Biology Assays: Essential Methods G. Kreitzer et al. 2010
2. The Microbial Challenge R.I. Krasner 2010
3. Microbiology: A Clinical Approach A. Strelkauskas et al. 2010
4. Immunology: a short course R. Coico & G. Sunshine 2009
5. Cell Biology Assay: Proteins G. Kreitzer et al. 2010
6. The Protein Hand Book J. M. Walker 2009
7. Basic Immunology, Functions &
Disorder of the Immune System
A.K Abbas & A.H.
Lichtman
2011
8. Poultry Diseases M. Pattison et al. 2008
9. Principles of Molecular Virology A.J. Cann 2005
10. Immunology: Understanding the
Immune System
K.D. Elgert 2009
11. Handbook of Microbiological Media R.M. Atlas 2010
12. Bacterial Virulence: Basic Principles,
Models and Global Approaches
S. Philippe 2010
13. Atlas of Immunology J.M. Cruse & R.E.Lewis 2010
14. Pathological Basis of Veterinary Disease M. Donald et al. 2007
15. Molecular Biology of the Cell B. Alberts et al. 2008
16. Kuby Immunology T.J. Kindt et al. 2007
17. Janeway’s Immunobiology K. Murphy et al. 2008
18. Veterinary Microbiology D.C. Hirsh et al. 2004
19. Cell Biology S. Tompkins 2010
20. Microarray Analysis of the Physical
Genome Methods & Protocols
J.R. Pollack 2009
21. Understanding Viruses T. Schuurs 2009
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22. Comprehensive Source Book of
Bacterial ProteinToxins
J.E. Alouf & M.R. Potoff 2006
23. Black’s Veterinary Dictionary E. Boden 2006
24. High-yield Immunology A.G. Johnson & B.L.
Clarke
2006
25. Gene Transfer, Delivery and Expression
Of DNA and RNA: A Laboratory Manual
T. Friemann& J. Rossi 2007
26. Immunogenicity of Bio-pharmaceuticals M .van de Weert &
E.H. Muller
2008
27. Basic Immunology, Functions and
Disorders of the Immune System
A.K. Abbas &
A.H. Lichtman
2009
28. Current Protocols: Essential Lab
Techniques
S.R. Gallagher &
E.A. Wiley
2008
29. Handbook of Media for Clinical
Microbiology
R.M. Atlas & J.W. Snyder 2006
30. Bacterial Pathogenesis: Methods and
Protocols
F.R. DeLeo & M. Otto 2008
31. Neuroimmunobiology: Natural
Immunity
L.Bertok & D.A.Chow 2005
32. Advanced Techniques in Diagnostic
Microbiology
Yi-Wei Tang & C.W.
Stratton
2006
33. Immunology of Fungal Infections G. D. Brown & M.G.
Netea
2007
34. Principles of Immunology- Students
Compendium
B.K. Sinha 2007
35. Cellular and Molecular Immunology A.K. Abbas et al. 2007
36. Janeway’s Immunobiology K. Murphy et al. 2008
37. The Vaccine Book B.R. Bloom & P. Lambert 2003
38. Gene Expression Systems J.M. Fernandez &
J.P. Hoeffler
1999
39. Methods in Microbiology: Microbiology
of Infection
S.H.E. Kauffmann &
D. Kabelitz
2002
40. Physiology and Bio-chemistry of
Prokaryotes
David O. White 2007
41. Immunotoxicology and
Immunopharmacology
R. Luebke et al. 2007
42. Molecular Genetics of Bacteria L. Snyder &
W. Champness
2007
43. Immunotherapy for Infectious Diseases J.M. Jacobson 2002
44. Immunosuppressant Analogs in C.V. Borlongan et al. 2003
Neuroprotection
45. Cytokine Knockouts G. Fantuzzi 2003
46. Alcamo’s Fundamentals of Microbiology J. Pommemlle 2007
47. Fields Virology Vol. I & II D.M. Knipe & P.M.
Howley
2007
Page 8
48. Escherichia Coli in Domestic Animals
and humans
C.L. Gyles 1994
49. Essential Genes B. Lewin 2006
50. Kuby Immunology T.J. Kindt et al. 2007
51. Methods for General and Molecular
Microbiology, 3rd Edition
C. A. Reddy, Terry J.
Beveridge, John A. Breznak, George Marzluf
2007
52. Fundamentals of Molecular Virology, 2nd
Edition
Nicholas H. Acheson 2011
53. The Ecology of Mycobacteria: Impact on Animals and Human Health, 2nd Edition
Jindrick Kazda, SvoParlik et al.
2009
54. Antimicrobial therapy in Swines, a
practical approach, 1st Edition
Lorenzo Fraile 2013
55. Molecular Medical Microbiology, Vol. I,
2nd Edition
Yi-Wei Tang, Max
Sussman et al.
2014
56. Molecular Medical Microbiology, Vol. II,
2nd Edition
Yi-Wei Tang, Max
Sussman et al.
2014
57. Veterinary Immunology: Principles and
Practice, 2nd Edition
Michael J. Day, Ronald D.
Schultz
2014
58. Immunology: A Short Course, 7th Edition Richard Coico, Geoffrey
Sunshine
2015
59. Concise Review of Veterinary
Microbiology, 2nd Edition
P. J. Quinn, B. K. Markey,
F. C. Leonard, E. S.
Fitzpatrick, S. Fanning
2015
60. Molecular Medical Microbiology, Vol. III,
2nd Edition
Yi-Wei Tang, Max
Sussman et al.
2014
61. Fenner's Veterinary Virology; 5th Edition N. James Maclachlan,
Edward J. Dubovi
2016
62. Kamadhenu Cows of India, 1st Edition Rajni Sekhri Sibal 2016
63. Objectives & Short Answer Questions
Veterinary Bacteriology & Mycology, 2nd Edition
B. S. Malik, C. S. Bakshi 2016
64. Microbiology: A Laboratory Manual, 11th
Edition
James G. Cappuccino,
Chad T. Welsh
2017
65. Prescott's Microbiology; 10th Edition Joanne Willey, Linda
Sherwood, Christopher J.
Woolverton
2017
66. Microbiology: An Introduction, 13th
Edition
Gerard J. Tortora, Berdell
R. Funke and Christine L.
Case
2018
67. Textbook of Medical Mycology, 4th edition Jagdish Chander 2018
68. Viruses, 1st Edition Paula Tennant, Gustavo
Fermin, Jerome Foster
2018
69. Kuby Immunology, 8th Edition
Jenni Punt, Sharon Stranford, Patricia Jones,
Judith A Owen
2018
Page 9
Awards/Recognitions:
S. No. Award/Recognition CAFT
Faculty
member
Conferred by/Agency Year
1 Commonwealth Academic Staff
Fellowship
Dr. Naresh
Kumar
Kakker
Commonwealth
Scholarship Commission, London,
U.K.
2008
2 Dr. P. Madhusudan Gold Medal
Award, IAAVR
Dr. Ajit
Singh
Indian Association for
Advancement of Veterinary
Research
2009
3 Best Teacher Award (ICAR
sponsored)
Dr. Ajit
Singh
CCS Haryana Agricultural
University, Hisar
2010
4 Commonwealth Academic Staff
Fellowship
Dr. Ajit
Singh
Commonwealth
Scholarship Commission, London,
U.K.
2010
5 Appreciation Letter, PD, AICRP on
FMD
Dr. Naresh
Kakker
Project Director, Foot-and- Mouth
Disease (ICAR), IVRI Campus
Mukteswar, Uttarakhand
2010
6 Fellow, Society For Applied
Biotechnology
Dr. Ajit
Singh
Society for Applied
Biotechnology
2011
7 Ist Prize, Best Regional
Research Station, AICRP on
FMD
Dr. Ravindra
Sharma & Dr.
Naresh
Kakker
Project Coordinator,
AICRP on FMD, IVRI,
Mukteshwar
2011
8 Honoured for delivering
Invited talk on “Foot-and-
Mouth Disease in India”
during “Brainstorming
workshop on Foot-and-
Mouth Disease”
Dr. Naresh Kumar Kakker
ICAR-CIRB, Sirsa
Road, Hisar
Septe
mber
14-15,
2018
9 Honoured for being the
“Rapporteur” in the
Technical Session during
3rd Convention of Vety.
Biochemists and
Biotechnologists and
National Symposium on
Bridging Biochemical
Interventions
Dr. Naresh Kumar Kakker
Pr.Scientist–
cum-HOD,
Veterinary Microbiology
and Incharge,
CCL, COVS, LUVAS
Directorate of HRM,
LUVAS, Hisar
November
3-4,
2018
10 Honoured for being invited
as Expert Speaker on “Status
of FMD in Haryana and
Delhi” during
“Brainstorming workshop on
Foot-and-Mouth Disease”
Dr. Swati
Dahiya,
Scientist
ICAR-CIRB, Sirsa
Road, Hisar
Septe
mber 14-15,
2018
Page 10
11 Best oral presentation award Dr. Akhil
Kumar Gupta,
Scientist
VI Annual
Convention of Society for Veterinary Science &
Biotechnology
held at College of Veterinary &
Animal Science,
Navania, Vallabhnagar,
Udaipur (Rajasthan).
13-14 Dece
mber
2018
12 Best oral presentation award Dr. Akhil
Kumar Gupta,
Scientist
37th Annual
convention of Indian society of
Veterinary Medicine held at
RAJUVAS, Bikaner
Feb.
1-3,
2019
Publications
1. Agnihotri, D., Singh, Y., Maan, S., Jain, V.K., Kumar, A., Sindhu, N., Jhamb, R., Goel, P., Kumar, A.
and Dalal, A. (2017). Molecular detection and clinico-haematological study of viral gastroenteritis in dogs. Haryana Vet. 56(1): 72-76.
2. Ahuja, S., Bhanot, V. and Mandal, B.S. (2019). Impact in knowledge gain of rural women through dairy
trainings .The Pharma Innovation Journal. 8(2): 351-353.
3. Batra, K., Nanda, T., Kumar, A., Gupta, A.K., Kumari, R., Kumar, V., Sheoran, N. and Maan, S. (2018).
Molecular Cloning and Characterization of Mx2 for Early Pregnancy Diagnosis in Bubalus bubalis. Proc.
Natl. Acad. Sci., India, Sect. B Biol. Sci. pp:1-14.
4. Bhanot, V., Ganguly, A. and Sharma, P. (2019). Hematological alterations and molecular detection of
theileriosis in crossbred cattle. Indian Journal of Animal Science. 89(6): 615–618.
5. Bhanot, V., Ganuly, A., Prakash, A. and Gupta, G. (2018). Comparative efficacy of Direct blood PCR
with microscopy for detection of Theileriosis in cross breed cattle. Indian journal of Comparative
Microbiology, Immunology and Infectious Disease.16-19.
6. Brar, R., Bhanot, V., Mahajan, N.K., Jindal, N. and Prakash, A. (2019). Retrospective studies on
epidemiology of chicken infectious anemia in broiler chicken in Haryana. Indian Journal of Poultry
Science. 54(1): 69−72.
7. Brar, R., Bhanot, V., Mahajan, N.K., Jindal, N., Prakash, A., Jangir, B.L. and Nehra, V. (2018). Chicken infectious anemia in layer chicken: epidemiological and pathological findings. The Haryana Veterinarian.
57(2): 128-131.
8. Brar, R., Bhanot, V., Mahajan, N.K., Tomar, P., Prakash, A. and Maan, S. (2019). Detection and molecular
characterization of chicken infectious anaemia virus in poultry flocks in Haryana. Indian Journal of
Animal Sciences. 89(12): 1309–1312.
9. Cheema, P.S., Singh, S., Kathiresan, S., Kumar, R., Bhanot, V. and Singh, V.P. (2017). Synthesis
recombinant p48 of Mycoplasma agalactiae by Site directed mutagenesis and its Immunological
characterization. Animal Biotechnology. 28(1): 11-17.
10. Dalal, A., Kakker, N.K., Kumar, A., Chaudhary, D., Bansal, N., Sindhu, N., Kumar, T., Maan, N.S. and
Maan, S. (2017). Whole genome sequence analysis of Bluetongue virus serotype 12 from Northen India.
Int. J. Curr. Microbiol. App. Sci. 6(12): 2346-2348.
11. Dalal, A., Kumar, V., Chaudhary, D., Bansal, N., Kumar, A., Kakker, N.K. and Maan, S. (2017). Past and
Present overview of “Orf”. Int. J. Curr. Microbiol..App.
12. Dalal, A., Maan, S., Kumar, A., Bansal, N., Kumar, V., Maan, N.S. and Kakker, N.K. (2017). Molecular
Analysis of Genome Segment-3 of Bluetongue Virus Serotype 12 Isolates from Haryana. Veterinary
World. 10(11): 1389-1393.
13. Dandasena, D., Bhandari, V., Sreenivasamurthy, G.S., Murthy, S., Roy, S., Bhanot, V., Arora, J.S., Singh,
S. and Sharma, P. (2018). A Real-Time PCR based assay for determining parasite to host ratio and
parasitemia in clinical samples of Bovine Theileriosis. Scientific Reports. 8: 15441. DOI:10.1038/s41598-
018-33721-3.
14. Dhundwal, K., Pander, B.L., Dalal, D.S., Magotra, A., Mittal, D., Singh, M., Malik, A. and Garg, A.
(2019). Characterization and validation of point mutation in MBL1 gene and its relationship with mastitis
in Murrah buffalo (Bubalus bubalis). Buffalo Bulletin. 38(3): 451-457.
15. Dutt, R., Singh, G., Singh, M., Sharma, M., Dalal, J. and Chandolia, R.K. (2017). Diagnosis of subclinical
endometritis in Murrah buffaloes through cytobrush technique. International Journal of Current Microbiology and Applied Sciences. 6(11): 494-499.
Page 11
16. Ganguly, A., Bisla, R.S., Ganguly, I., Singh, H., Bhanot, V. and Chaudhiri, S.S. (2017). Direct Blood
PCR detection of Babesia bigemina and its effect on haematological and biochemical profile in crossbred
cattle of eastern Haryana. Indian Journal of Animal research. 51(1): 141-145.
17. Ganguly, A., Bisla, R.S., Singh, H., Bhanot, V., Kumar, A., Kumari, S., Maharana, B.R. and Ganguly, I.
(2017). Prevalence and haemato-biochemical changes of tick borne haemoparasitic diseases in crossbred cattle of Haryana, India. Indian Journal of Animal Sciences. 87(5): 552-557.
18. Jhambh, R., Dimri, U., Gopalakrishnan, A., Singh, M. and Chhabra, R. (2017). Prevalence and risk factors
of subclinical mastitis in buffaloes at an organised dairy farm in western Haryana. The Haryana
Veterinarian. 56(2): 189-193.
19. Kumar, A., Nanda, T., Kumar, V., Chhabra, R., Mittal, D., Singh, M. and Maan, S. (2017). Isolation and
identification of Brucella species from aborted foetuses of crossbred cattle. The Haryana Veterinarian.
56(2): 184-188.
20. Kumar, V., Maan, S., Kumar, A., Batra, K., Chaudhary, D., Dalal, A., Gupta, A.K., Bansal, N., Sheoran,
N. and Maan, N.S. (2017). Real time PCR assay for differentiation of Brucella abortus and Brucella
melitensis. Indian Journal of Animal Research. doiDoi:10.18805/ijar.v0iOF.8464.
21. Kumari, R., Kumar, V., Batra, K., Kumar, A., Bansal, N., Dalal, A., Maan, S. and Nanda, T. (2019).
Development of sybr green chemistry based real time PCR for validation of sperm sex ratio in buffalo
bull semen. Ruminant Science. 8(1): 39-44.
22. Maan, S., Kumar, A., Gupta, A.K., Dalal, A., Chaudhary, D., Gupta, T.K., Bansal, N., Kumar, V., Batra,
K., Sindhu, N., Kumar, A., Mahajan, N.K., Maan, N.S. and Mertens, P.P.C. (2017). Concurrent infection
of Bluetongue and Peste-des-petits-ruminants virus in small ruminants in Haryana state of India. Transboundary and emerging diseases. DOI: 10.111/tbed.12610.ISSN:1865-1682.
23. Maan, S., Tiwari, A., Chaudhary, D., Dalal, A., Bansal, N., Kumar, A., Kakker, N.K., Kumar, V., Batra,
K. and Maan. N.S. (2017). A comprehensive study on sero-prevalence of bluetongue virus in Haryana
state of India. Veterinary World. 10(11): 1464-1470.
24. Minhas, S.K., Kamble, N., Dey, S., Madhan, M.C. and Kataria, J.M. (2017). High-yield expression and
purification of recombinant σB protein of indigenous avian reovirus in prokaryotic system. International
journal of Current Microbiology and Applied sciences. 6(9): 434- 441.
25. Mittal, D., Sharma, A., Singh, M., Rajesh and Mahajan, N.K. (2018). Antimicrobial sensitivity pattern observed in microbes associated with bovine mastitis. The Haryana Veterinarian. 57(2): 215-218. Sci.
6(12): 2159-2173.
26. Sharma, A., Chhabra, R., Singh, M. and Charaya, G. (2018). Prevalence, etiology and antibiogram of
bacterial isolates recovered from mastitis of buffaloes. Buffalo Bulletin. 37(3): 313-320.
27. Sharma, S.K., Meena, D.S., Nathawat, P., Yadav, R., Mehta, S.C. and Kataria, A.K. (2018). Detection
of coagulase production variability among Staphylococcus aureus isolates of human and animal origins.
Veterinary Practitioner. 19(1): 1-4.
28. Sharma, S.K., Meena, D.S., Yadav, R., Meena, D. and Nathawat, P. (2017). Multidrug Resistance Pattern of Escherichia coli Isolates Obtained from Healthy and Diseased Camel (Camelusdromedarius). Journal
of Camel Practice and Research. 24(3): 275.
29. Singh, A. (2017). Biotechnological applications for Ayurvedic medicines of plant origin: Editorial
concept note. J. AYUSH 1:1.
30. Singh, A. (2018). Immunogenicity testing in the era of reverse and systems vaccinology. Vet. Immunol.
Biotechnol. 1(2): 3-11.
31. Singh, A. (2018). Immunology as applied to farm animal welfare: General principles and interventions. Res. & Rev.: J. Immunol. 8(1):1–13.
32. Singh, A. (2018). Next generation immunoassays for infectious diseases of animals. Res. & Rev.: J.
Immunol. (RRJoI). 7(2): 22-43.
33. Singh, M., Yadav, P., Sharma, A., Garg, V.K. and Mittal, D. (2017). Estimation of mineral and trace
element profile in bubaline milk affected with subclinical mastitis. Biological Trace Element Research.
176(2): 305-310.
34. Tomar, P., Singh, Y., Mahajan, N.K., Jindal, N. and Singh, M. (2017). Duplex PCR for direct detection
of Mycoplasma gallisepticum and Mycoplasma synoviae from tissues of poultry affected with respiratory infections. International Journal of Livestock Research. 7(11): 280-286.
35. Tomar, P., Singh, Y., Mahajan, N.K., Jindal, N. and Singh, M. (2017). Molecular detection of avian
Mycoplasmas in poultry affected with respiratory infections in Haryana (India). International Journal of
Current Microbiology and Applied Sciences. 6(6): 2155-2162.
36. Yadav, R., Yadav, J., Gahlot, K., Purva, M., Deora, A., Kumar, P., Nathawat, P., Rathore, N.S.,
Maherchandani, S. and Kashyap, S.K. (2017). Repetitive Extragenic Palindromic-PCR (REP-PCR) typing
of Campylobacter jejuni isolated from poultry. Veterinary Practitioner. 18(2): 160-163.
Page 12
37. Yadav, R., Yadav, J., Maherchandani, S. and Kashyap, S.K. (2018). Typing of Campylobacter jejuni
isolated from poultry on the basis of flaA-RFLP by various restriction enzymes. Journal of Veterinary and
Animal Science. 6: 1–5.
38. Yadav, V., Singh, A. and Rawat, J. (2017). Characterization of a broadly specific LPS-neutralizing
single domain antibody. Res. & Rev.: J. Immunol. 7(1): 1-10.
39. Maan, S., Maan, N.S., Batra, K., Kumar, A., Gupta, A.K., Rao, PP., Hemadri, D., Reddy, Y.N.,
Guimera, M., Belaganahalli, M.N. and Mertens, P.P.C. (2016). Reverse transcription loop-
mediated isothermal amplification assays for rapid identification of eastern and western strains
of bluetongue virus in India. J. Virol. Methods. 234: 65-74.
40. Kumar, L., Batra, K., Chaudhary, D., Gupta, A.K., Dalal, A., Kalyanaraman, B., Irulappan, G.P.,
Kumar, V. and Maan, S. (2016). Full-Genome Sequence Analysis of a Reassortant Strain of
Bluetongue virus Serotype 16 from Southern India. Genome. Announc. 4(4): e00783-16.
doi:10.1128/genomeA.00783-16.
41. Singh, M., Sharma, A., Kumar, A., Mittal, D., Kumar, P. and Charaya, G. (2016). Relative
expression of proinflammatory cytokines by Real Time PCR in milk somatic cells of subclinical
mastitis affected buffaloes. The Indian Journal of Animal Sciences. 86(9): 991–993.
42. Singh, M., Sharma, A., Mittal, D., Yadav, P. and Charaya, G. (2016). Assessment of lactate
dehydrogenase enzyme activity in milk as a marker for detection of subclinical mastitis. Journal
of Animal Research. 6(2): 113-116.
43. Gupta, R., Jindal, N., Arora, D., Singh, M. and Kapoor, P.K. (2016). Detection of Salmonella
Typhimurium, Salmonella Enteritidis and Salmonella Gallinarum from suspected cases of Fowl
Typhoid in poultry in Haryana. The Indian Veterinary Journal. 93(7): 44-47.
44. Sharma, V., Jakhar, K.K. and Dahiya, S. (2016). Immuno-pathological studies on broiler
chicken experimentally infected with Escherichia coli and supplemented with neem
(Azadriachta indica) leaf extract. Vet World. 9(7): 735-741.
Books/E-books &chapters:
Yadav, J., Yadav, R. and Yadav, R. (2019). Haemoprotozoan Disease in Cattles. Book title:
Cattle health concern in India. Agrobioos online. ISBN : 9788193467398.
Yadav, R., Yadav, J., Diwakar, R.P. and Mehra, U.S. (2019). Haemorrhagic Septicaemia.
Book title: Cattle heatlth concern in India. Agrobioos online. ISBN: 9788193467398.
Manuals & chapters inmanuals:
Kumar, P. and Minhas, S.K. (2018). Characterization of cell lines. Manual of 32nd CAFT, Veterinary Microbiology, LUVAS pp. 15-18.
Minhas, S.K., Dalal, A., Kumar, P., Sharma, A. and Dahiya, S. (2018). Culture environment, media and
supplement for animal cell culture. Manual of 32nd CAFT, Veterinary Microbiology, LUVAS pp. 80-84.
Dalal, A., Minhas, S.K., Kakker, N.K. and Dahiya, S. (2018). Quantitation, cryopreservation and revival
of cell culture stocks. Manual of 32nd CAFT, Veterinary Microbiology, LUVAS pp. 107-111.
Kakker, N.K. and Kumari, S. (2019). An overview of Endemic, Emerging and Re-emerging diseases of
Veterinary Importance. Manual of 33rd CAFT, Veterinary Microbiology, LUVAS pp. 1-8.
Dalal, A., Kumari, S. and Kakker, N.K. (2019). Sampling and transportation of clinical material from field
to laboratory for disease diagnosis. Manual of 33rd CAFT, Veterinary Microbiology, LUVAS pp. 13-20.
Lather, A., Dahiya, S., Gupta, A.K. and Kumari, S. (2019). Cell mediated immunity based diagnostic assays for the infectious diseases of veterinary importance. Manual of 33rd CAFT, Veterinary Microbiology,
LUVAS pp. 72-76.
Kumar, P., Lather, A., Kumari, S. and Kaur, R. (2019). Recent approaches for development of reverse
genetics system for Pestivirus. Manual of 33rd CAFT, Veterinary Microbiology, LUVAS pp. 87-92.
Page 13
Kumari, S., Lather, A., Kumar, P. and Dalal. A. (2019). Isolation of viruses in Embryonaqted Chicken
Eggs and Preparation Of Chicken Embryo Fibroblast (CEF) culture. Manual of 33rd CAFT, Veterinary
Microbiology, LUVAS pp. 122-127.
Kumar, P., Kumari, S. and Kaur, R. (2019). Bioinformatics tools to study molecular epidemiology of
emerging veterinary pathogens. Manual of 33rd CAFT, Veterinary Microbiology, LUVAS pp 159-161.
Chhabra, R., Yadav, R., Singh, M. and Charaya, G. (2018). Mastitis: An overview. Published in training
manual “Hands on Training on Mastitis” held at College Central Laboratory from May 15-18, May 22-
25, May 29 –June 1, 2018. pp: 1-11.
Kumar, P., Chhabra, R., Yadav, R., Singh, M. and Charaya, G. (2018). Diagnosis of mastitis in dairy
animals. Published in training manual “Hands on Training on Mastitis” held at College Central
Laboratory from May 15-18, May 22-25, May 29 –June 1, 2018. pp: 13-18.
Yadav, R., Singh, M., Chhabra, R. and Kumar, P. (2018). Mastitic milk sample collection and processing.
Published in training manual “Hands on Training on Mastitis” held at College Central Laboratory from
May 15-18, May 22-25, May 29 –June 1, 2018. pp: 19-21.
Chhabra, R., Yadav, R. and Singh, M. (2018). Cleaning, packing and sterilization of laboratory glassware.
Published in training manual “Hands on Training on Mastitis” held at College Central Laboratory from
May 15-18, May 22-25, May 29 –June 1, 2018. pp: 23-26.
Yadav, R., Singh, M. and Chhabra, R. (2018). Media preparation for bacteriological examination.
Published in training manual “Hands on Training on Mastitis” held at College Central Laboratory from
May 15-18, May 22-25, May 29 –June 1, 2018. pp: 27-31.
Singh, M., Yadav, R. and Chhabra, R. (2018). Identification of bacteria by colony morphology and staining
procedures. Published in training manual “Hands on Training on Mastitis” held at College Central Laboratory from May 15-18, May 22-25, May 29 –June 1, 2018. pp: 33-36.
Chhabra, R., Yadav, R. and Singh, M. (2018). Microbial identification using BiomerieuxVitek 2 system.
Published in training manual “Hands on Training on Mastitis” held at College Central Laboratory from May 15-18, May 22-25, May 29 –June 1, 2018. pp: 37-40.
Yadav, R., Chhabra, R. and Singh, M. (2018). Antibiotic sensitivity test of mastitic milk samples. Published
in training manual “Hands on Training on Mastitis” held at College Central Laboratory from May 15-18, May 22-25, May 29 –June 1, 2018. pp: 41-42.
Yadav, R., Chhabra, R. and Singh, M. (2018). Microbial identification by VITEK 2 system. Published in
training manual “Techniques in Molecular Biology and Genetic Engineering” held at Department of Animal Biotechnology from March 6-26, 2018. pp: 55-57.
Singh, M., Chhabra, R. and Yadav, R. (2018). Isolation, identification and antimicrobial sensitivity testing
of bacterial pathogens. Published in training manual “Techniques in Molecular Biology and Genetic Engineering” held at Department of Animal Biotechnology from March 6-26, 2018. pp: 55-57.
Chhabra, R., Singh, M. and Yadav, R. (2018). Flow cytometry analysis of lymphocyte populations from whole blood. Published in training manual “Techniques in Molecular Biology and Genetic Engineering”
held at Department of Animal Biotechnology from March 6-26, 2018. pp: 58-59.
Chhabra, R., Kundu, P., Krishan, B., Yadav, R. and Talukdar, S.J. (2018). Estimation of the CD4+ and CD8+ Cell Population of Chicken Blood By Flow Cytometry. i.b.i.d. pp: 103-104.
Chapters in conference/symposium compendium etc.
Chhabra, R., Shrinet, G., Dahiya, S., Yadav, R. and Singh, M. (2019). Phage therapy as an alternative
strategy: to combat AMR due to bovine mastitis. 19th Indian veterinary congress, XXVI Annual
conference of IAAVR and national symposium on ‘Innovative progress in animal health and production for safe and secured food under one health prospective’ held on 1-2 February 2019, at WBAFS, Kolkata,
West Bengal, pp: 69.
Any other: Compact Disks of Manuals
Page 14
1. Gupta, A.K., Singh, M. and Lather, A. (2019). Editors. Compact Disc of 33rd CAFT Course Manual
of the course entitled ‘Microbiological Techniques for Diagnosis of Emerging and Re-
emerging diseases of Veterinary Importance’, Deptt. of Veterinary Microbiology, LUVAS, Hisar, Haryana, Nov. 20 – Dec. 10, 2019.
2. Dahiya, S. and Chhabra, R. (2018). Editors. Compact Disc of 32nd CAFT Course Manual of the
course entitled ‘Recent Advances in Cell based Technologies for Animal Disease
Diagnostics and Therapeutic’, Deptt. of Veterinary Microbiology, LUVAS, Hisar, Haryana,
October 11 to 31, 2018.
3. Chhabra, R. and Kakker, N.K. (2018). Editors. Compact Disc of 31st CAFT Course Manual of the
course entitled ‘Diagnosis and Control of Emerging and Re-emerging Trans-boundary
Diseases of Poultry’, Deptt. of Veterinary Microbiology, LUVAS, Hisar, Haryana, February 7 to
27, 2018.
4. Kakker, N.K. and Chhabra, R. (2017). Editors. Compact Disc of 30th CAFT Course Manual of the
course entitled ‘Microbial Genomics and Proteomics in Diagnosis and Control of Diseases of Veterinary Importance’, Deptt. of Veterinary Microbiology, LUVAS, Hisar, Haryana,
November 7 to 27, 2017.
5. Singh, A. and Kadian, S.K. (2017). Editors. Compact Disc of 29th CAFT Course Manual of the
course entitled ‘Modern Technologies for Production and Applications of Antibodies for
Animal Health Improvement’, Deptt. of Veterinary Microbiology, LUVAS, Hisar, Haryana, March 1 to 21, 2017.
Financial Statement
Expenditure under CAFT during 2016-20
Head 2016-17 2017-18 2018-19 2019-20*
Operating cost
of Training
352367 938988 324044 356400
Recurring
Contingency
335838 382250 383097 ----
Non- Recurring
Contingency/T. A.
---- 3132 32254 ----
Library ---- 12127 26663 ----
Total 688205 1336497 766058 1798000*
(*allotted)
Dr. N. K. Kakker
Pr. Scientist & Head-cum-
Director ICAR-CAFT
Deptt. of Veterinary Microbiology LUVAS, Hisar-125004 (Haryana)
