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7/29/2016
1
Chapter 9
CELLULAR RESPIRATION
Relationship of Processes
Respiration: exergonic (releases E)
C6H12O6 + 6O2 6H2O + 6CO2 + ATP (+ heat)
Photosynthesis: endergonic (requires E)
6H2O + 6CO2 + Light C6H12O6 + 6O2
Cellular Respiration
complex process whereby cells make ATP by breaking
down organic compounds(glucose)
many rxns in aerobic respiration are redox: (one
reactant is oxidized while another is reduced)
location: mitochondrial cristae
Oxidation and Reduction
loses e- (donor)
gains e- (acceptor)
Not all electrons are transferred from one
substance to another, some change the degree of
e- sharing in covalent bonds.
Glucose Catabolism
Catabolic Pathway
Complex organic Simpler waste molecules products with less E
Some E used to do work
and dissipated as heat
Oxygen is extremely electronegative
Oxidation of glucose transfers e- to lower energy state-
does main work of respiration
releases energy to make ATP
** done in series of steps**
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Moving Electrons
Electrons cannot move alone in cells
• Move as part of H atom
• Energy is released as electrons “fall” from organic
molecule to O2.
Electron Carriers • Move electrons by shuttling H atoms around
NAD+ NADH
FAD+2 FADH2
NAD+
• Coenzyme (nicotinamide adenine dinucleotide)
• Electron acceptor- carries electrons to ETC
• Acts as an oxidizing agent during respiration
• Dehydroginase(enzyme)- catalyzes NAD+ NADH
NAD animation
Electron Transport Chain
• ETC breaks the fall of electrons of oxidation of
glucose into series of smaller steps
• Released energy is used to make ATP
• Extra energy is released as heat.
Glycolysis (glucose/splitting)
GLUCOSE(6C)is partially oxidized
end products: 2 PYRUVATE(3C), 2 NADH, 2 ATP
- occurs in cytosol
- 1st step: always occurs before
respiration or fermentation
- occurs in the absence of oxygen
- ancient pathway (early prokaryotes)
- inefficient
Glycolysis Overview
Stage 1: Energy Investment
• Cell uses ATP to
phosphorylate glucose
• Endergonic
Stage 2: Energy Payoff
• Two 3-C compounds oxidized
• Exergonic
Yield
2 pyruvate
2 NADH
2 ATP- substrate-level
phosphorylation
- Phosphate comes from sugar
substrate(PEP), not ETC
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Steps of Glycolysis
glycolysis animation
2 Possible Pathways for Pyruvate
If O2 present If no O2 present
respiration fermentation
(aerobic) (anaerobic respiration)
mitochondria cytosol
Stages of Cellular Respiration
1. Glycolysis
2. Citric Acid(Krebs)Cycle
3. Oxidative Phosphorylation
Cellular Respiration(aerobic)
C6H12O6 + 6 O2 6 H2O + 6 CO2 + 32 ATP
Process of breakdown of pyruvate in the
presence of oxygen
- prokaryotic cells: occurs in cytosol
- eukaryotic cells: occurs in mitochondria
- much more efficient than anaerobic
respiration
2 Major Stages of Respiration
1. Citric Acid Cycle
- oxidation of glucose is completed
- NADH and FADH2 are produced
2. Electron transport chain
- NADH is used to make ATP via oxidative phosphorolation
- location where most ATP is made
Location of Processes
Citric Acid Cycle
(matrix)
ETC
(inner
membrane of
cristae)
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Pyruvate oxidized to Acetyl CoA
Occurs before the Citric Acid Cycle
• 3 step oxidation process
• NAD+ reduced to NADH (acetate formed)
• Coenzyme A attached to acetate to form Acetyl CoA
• CO2 by-product is released into atmosphere
Pyruvate + CoA + NAD+ Acetyl CoA + CO2 + NADH
Citric Acid Cycle (Krebs Cycle) 2 pyruvate from glycolysis enter cycle
Occurs in mitochondrial matrix
Glucose is fully oxidized
animation
Oxidative Phosphorylation (production of ATP)
Electron Transport
Chain
• Occurs in inner membrane
of mitochondria
• Produces ATP by
oxidative
phosphorylation via
chemiosmosis
Chemiosmosis
• H+ ions pumped across
inner mitochondrial
membrane
• H+ ions diffuse through
ATP synthase to make
ATP
Electron Transport Chain
• Collection of molecules embedded in
inner membrane of mitochondria
• Tightly bound protein complexes
• FADH2 and NADH donate e- for
electron transport (redox rxns)
for ATP synthesis
• Does not make ATP directly, ATP
made through chemiosmosis
• O2 is final e- acceptor from H+
to form H2O
Oxidative Phosphorylation
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Energy Coupling of Chemiosmosis
How ATP Synthase Works
• H+ ions enter stator (1/2
channel) in membrane
• H+ ions enter rotor- changes
shape of subunits
– Rotor spins within membrane
• Each H+ ion makes one complete
turn
– Passes into matrix
• Turning of rod produces ATP from
ADP and Pi
animation ETC
Fermentation = glycolysis + regeneration of NAD+
1. Lactate Fermentation (animals)
- pyruvate converted to lactate
A. NADH is oxidized and donates its H to pyruvate
B. resultant NAD returns to glycolysis where it is reduced to NADH
C no release of CO2
** 2 ATP formed **
** process is CYCLICAL**
**lactate eventually diffuses into liver where it is converted back to pyruvate
when O2 again present **
2. Alcoholic Fermentation (yeasts, plant cells, microorganisms)
- pyruvate converted to ethanol
A. CO2 molecule is removed from pyruvate (3C) forming acetaldehyde
(2 C) B. acetyldehyde is reduced to form ethanol
2 H (from NADH + H ion) are added to 2C compound to form
ethanol
C. NAD is formed (back to glycolysis)
** 2 ATP formed **
** process is CYCLICAL**
**causes alcohol in beer and wine,
air bubbles in bread, beer, and wine**
NO ATP FORMED IN
FERMENTATION
PURPOSE OF FERMENTATION:
TO REGENERATE NAD FOR GLYCOLYSIS
2 ATP produce enough energy for prokaryotes and small
multi-cellular eukaryotes
Useful for anaerobes and facultative anaerobes
Very widespread metabolic pathway of Earth’s organisms
Alternative Energy Sources
What if the body runs out of sugar for
glycolysis? Can the body still make ATP?
YES
This is how the Atkins and South Beach diets
work.
They are low carb, high protein/fat diets.
Alternative Energy Sources
Fats as fuel:
• Glycerol PGAL glycolysis
• FA tails Acetyl CoA Krebs
Proteins as fuel:
• proteins amino acids
• Amino acids pyruvate or
acetyl CoA Krebs
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review animation
Biofeedback Regulation of
Cellular Respiration
Importance of Phosphofructokinase:
(regulates rate of respiration)
• Allosteric enzyme- receptors for
specific inhibitors and activators
• controls rate of glycolysis and
citric acid cycle
• Inhibited by ATP and citrate
citrate citric acid cycle
ATP glycolysis
• Stimulated by AMP
• AMP+ P + P ATP
Review of Respiration
Glycolysis
Citric Acid Cycle
Oxidative Phosphorylation
Electron Transport Chain Chemiosmosis