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8/12/2019 Concept Map Iuk107
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Other Chem WindowIUK107 Part 2Other Window of Chemistry for Technologists1. Materials: synthetic (IUK 107 Part 1) & natural
(IUK 107 Part 2)
2. Trendy natural: Biomass3. Inorganic component is naturally contained in
biomass
4. Analysis should include inorganics: Uv-vis,AAS, ICP, EDAX.
5. Previous organic analysis included: IR, NMR,(+GC-MS)
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Concept MapIUK107 Part 2Other Window of Chemistry for Technologists
5 December 2013
1.Organic & Inorganic Samples:
Preparation Concept & Analyses
2Touch up on MS of HalogenatedCompounds
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INORGANICS: Biomass & Materials
New: fungus on keratinised tissue:
Touch on sample preparation: matrix + filler
Scanning Electron Microscopy with Energy DispersiveAnalysis of X-ray
Light & Absorption as Materials Response to Radiation
Summary - InorganicsIUK107 Part 2 Other Window of Chemistry for Technologists
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Towards relevance in intellectual capacity:
Uv-visible Spectroscopy
Atomic Absorption Spectroscopy
Inductively Coupled PlasmaQuiz 1
Group Presentation - excellent collaborative workalthough too detail.
SummaryIUK107 Part 2 Other Window of Chemistry for Technologists
UV-VIS AAS ICP
Source Uv-vis light HCL Plasma
Sample Response (many effects) Atomise Ionise
Power Low E radiation Furnace: 2000oC
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INORGANICSupposedly
Sample preparationIUK107 Part 2 Other Window of Chemistry for Technologists
Sample can containmixture of elements
Name 1 that
experience
inteference if in
mixture
Normally Quantitative
-calibration curve
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ORGANICSample preparation
IUK107 Part 2 Other Window of Chemistry for Technologists
Borrowed pic
dont
confuse
Consequence
Required due to
instrument limitation at
handling mixture
Name one technique
thats capable
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SEM/EDAX: Electron image | EDX: Intensity vs energy(keV)
UV-VIS: 200-800 nm; analyte responses: A vs l (nm)
AAS: HCL source; atomisation; atoms A;A vs l (nm)
ICP: Plasma source and ioniser; ions A;A vs l (nm)
Spectral ResponsesIUK107 Part 2 Other Window of Chemistry for Technologists
02/12/2013 8Concept Map - Part 2 IUK107 - Other ChemWindow for Technologists
IR: wavelength =0.78-1000 mm: % transmittance vs 1/l (cm-1 :
400-4000)
NMR: nucleus resonance; probe dependent; Intensity vschemical shift (ppm)
GC-MS: GC Separation:intensity vs tR | MS Fragment:
Abundance vs m/z (speciation)
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Uv-visible SpectroscopyAll - colour
Atomic Absorption Spectroscopy
Inductively Coupled Plasma
SEM/EDXS, organic, inorganic-EDX Infra-red Spectroscopy:L/S/G
Nuclear Magnetic Resonance: PROBE - L/S
Gas Chromatography-Mass Spectrometry L/G
volatile, stable
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IR Scan the functional group of a molecule
Pure or reference/standard/expertise/familliarity required
NMR structural elucidation
Pure or simple mixture
MS verify/confirm elucidated structure
Must pure or need standards
Stepwise Analysis - organicsIUK107 Part 2 Other Window of Chemistry for Technologists
Functionalregion: 1500-
4000 cm-1
Fingerprint:500-1500 cm-1
Atlas/Database match
Mixture is ridiculous
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Uv viswherecolouris present
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SEM/EDXCheck types of elements
Proceed with quantitative techniques
AASDecide on hollow cathode lamp
Prepare standards
Find sample digestion procedure
ICPAllows us to skip SEM/EDX and AASTo some extent, ICPMSrules out theneed for XRD, XRF, XPS
Stepwise Analysis InorganicsIUK107 Part 2 Other Window of Chemistry for Technologists
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Uv viswherecolouris present
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Start off: SEM/EDX Imageidentify variation (# of
forms)
Semiquantitative via EDX Check the types of elements
present
Check presence of C
If nonecheck ash %
If C present, analysis of inorganicsrequires ashing procedure
Nextproceed woth analysis.
Stepwise Analysis Totally unknown (s l)IUK107 Part 2Other Window of Chemistry for Technologists
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If (l): Start
off: IR for
functional
group.
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CH2Cl2 Calculate MW. This is for M+
Check radioisotopic probability:
M+ and all M++2
Abundance: 50:50% (note complexity)
Draw:
Indication:
IUK107 - Part 2. Separation and Speciation:GC-MS
13
Important Examples
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Mass SpectroscopyIUK107 Part 2Other Window of Chemistry for Technologists1. CH2CCl2
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CH2Br2 Calculate MW. This is for M+
Check radioisotopic probability:
M+ and all M++2
Abundance: 50:50% (note complexity)
Draw:
Indication:
IUK107 - Part 2. Separation and Speciation:GC-MS
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Important Examples
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Mass SpectroscopyIUK107 Part 2Other Window of Chemistry for Technologists1. CH2Br2
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QuestionsIUK107 Part 2Other Window of Chemistry for Technologists
1. Quiz 1
2. Quiz 2
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Concept Map - togetherIUK107 Part 2Other Window of Chemistry for Technologists
02/12/2013 18Concept Map - Part 2 IUK107 - Other ChemWindow for Technologists