Content

Plenary Lecture 1

FL-1 Insect fungi: development of insects for the bioprospecting of novel

metabolites 3

Drion G. Boucias

PL-2 The application of fluorescence in-situ hybridisation (FISH) for microbial

community structure and architecture determination in the activated sludge

process 5

Linda Blackall

Basic and applied science: The pharmaceutical and pharmacological expertise

of the University of Kuopio, Finland 7

Pekka Hyvonen

Medical Biotechnology I : Post Genomic Era 11

IL-Medical 101 A new class of potent antimalarial drugs based on anti-phospholipid

effectors. A need for post-genomic based technologies to identify the

pharmacological target 13

HENRI VIAL

Genomic approach and bioinformatics in searching for genes involved in clinical

malaria 15

Anavaj Sakuntabhai

Melioidosis genome project (Only title shown)

Sirirurg Songsivilai

Genome project and cancer research in Thailand 17

Apiwat Mutirangura

Medical Biotechnology II: Diagnostic Test Kit 19

IL-Medical 1101 Progressing of diagnostic test kit industry in the international level 2 1

Bernd Koenig

IL-Medical 1102 Invention of ready-to-use diagnostic test kits for tropical infections

(Only Title shown)

Wanpen Chaicumpa

IL-Medial 1103 Leptospira identification test kit 2 3

Suttipant Sarasombath

IL-Medical 1144 PCR kit for detection of Salmonella (Only title shown)

Watanalai Panbangred

Food Technology: Functional Food for Good Health

iL-FOOd-01 Potential and growth opportunities in functional food

Pisuth Lertvilai

ILfOOd-02 Regulations on dietary supplement

Chanin Charoenpong

lL-F0&03 Ingredients for functional food and nutraceutical food

Tuangthip Aroonpairoj

I L f c d C 4 Challenges of GMF for higher nutritional quality foods

Sakarindr Bhumiratana

Seed Biotechnology 33

ILSeed4l Trend in seed industry in Asia-Pacific (Only title shown)

Manas Chiaravanond

I L S ~ ~ ~ M Seed quality and quality assessment 3 5

M. J. Hill

IL-Sed-03 Utilization of modern technology and biotechnology in corn seed industry

in the Asia-Pacific 36

Tunya Kunta

l~Seed-04 DNA technology in seed industry 37

Somvong Trakoonrung

Conference on Functional Genomics of Rice 39

F'LRiceOl Developing rice as a cereal genomics model 4 1

Stephen Goff

F'L-Ria302 Current status of genome sequencing and concerted progress of functional

genomics at the rice genome research program 4 2

Takuji Sasaki

Opportunities and challenges for biotechnology transfer to developing countries:

An evolving perspective from private sector 4 3

Shanthu Shantharam

Post-genomics era and application in Thailand 44

Apichart Vanavichit

The ground zero of rice genome in Thailand: tagging, mapping

and breeding efforts 4 5

Theerayut Toojinda

Rice P-glucosidase genomics, identification and characterization 4 7

James R. Ketudat Cairns

Biodiversity of the rice blast pathogen, Pyricularia grisea, and their

interaction with the resistant genes

Prasartporn Smitamana

Effect of extracellular product of Skeletonema costatum on growth of

Vibrio harveyi 66

Orapin Khongpakdee

Effects of four natural foods on spawning of banana shrimps

(Penaeus merguiensis De Man) 6 7

Chairat Pumchouy

(XShrimp-12 Effects of production conditions on chitosan deacetylation yield 6 8

Piyabutr Wanichpongpan

National Conference on Starch Technology 69

p~starch-I Structure of amylopectin and its effect on starch properties 7 1

Jay-lin Jane

\LStalrh-01 Isolation and characterisation of Pachyrhizus ahipa

(Andean yam bean) starch 7 2

Wolfgang Bergthaller

Grain amaranth - integrated development of starch and other co-products 74

Harold Corke

Survey of amylose content in naked barley (H. vulgare. Nudum) 7 5

A. Mohammadkhani

Preparation of cationic starch by dry process 76

Ma Chengfeng

Modification of tapioca starch by acetylation for frozen food products 77

nanong Naivikul

Utilization of the combined crosslink-acid modified tapioca starch for the

pharmaceutical industry 78

Saiya vit Varavinit

Endothermic transitions of acid-modified tapioca starches; annealing and

freeze-thaw stability study 7 9

Napaporn Atichokudomchai

Preparation and properties of small-particle cassava starch 80

Varangkana Sanguanpong

Rheological characterization and modeling of the gelatinization of a starch

dispersion 81

Jirarat Tattiyakul

Mathematical modeling of structural relaxation of selected sugars 82

Ratchapong Wungtanagorn

JSPS Seminar I : Microbial Utilization of Organic Waste Resources83

JSPSCOl Construction of a complex microbial community which can degrade

rice straw effectively 8 5

Yasuo Igarashi, Z. Cui, S. Haruta, and M. lshii

Cloning of Aspergillus aculeatus P-mannosidase gene and its

overproduction in Aspergillus oryzae 86

Motoo Arai, Shin Kanamasa, Goro Takada, Jun-ichi Sumitani, and Takashi

Kawaguchi

Production of P-glucosidase in the cassava-liquid waste and velvet bean

(Mucuna pruriens DC) 8 7

Kapti Rahayu Kuswanto and Sri Wedhastri

Suitability of decomposed empty oil palm empty fruit bunch ( E F B )

by Aspergillus terreus SUK-1 for cultivation of mushrooms 8 8

Daud, F., Chiang, F. P., Senafi, S., Kader, J. and Omar, 0 .

Expression of chitinase and hydrogenase genes in

Clostridium paraputrificum for enhancing hydrogen gas production 89

Kunio Ohmiya, Kenji Morimoto, Dwierra Evvyernie, Michiko Yoshimoto, Tetsuya

Kimura, and Kazuo Sakka

Trend in using fungal enzymes, lignin- and pectin-degrading enzymes, in

improvement of the paper mulberry pulping process 9 0

Phuntip Poonpairoj, Churapa Peerapatsakul and Lerluck Chitradon

Paper Presentation only

Cloning of the poly(L-lactic acid) depolymerase gene (pld) from

the chromosome of Amycolatopsis sp. strain K104-1 9 1

Emiko Matsuda, Kohei Nakamura, Toshio Tomita, Naoki Abe, and Yoshiyuki

Kamio

The use of a high specific activity and high level of crude

cellulase-free-xylanase from a thermophilic fungus in pulp bleaching 92

N. S. Mendoza, L. A. Mabilangan, T. S. Caliwara and N. M. Unciano

Application of alkaline xylanolytic enzymes of alkaliphilic Bacillus sp. K-I in pulp

prebleaching process 93

Khanock Ratanakhanokchai and Khin Lay Kyu

Cellulolytic and hemicellulolytic activity of thermophilic bacteria to degrade

agricultural waste 95

Yantyati Widyastuti, Kusmiati, Shanti Ratnakomala, Tresnawati Purwadaria, Esti

Utarti

Utilization of chitin by Bacillus thuringiensis subsp. kurstaki HDI(G) 9 6

Chanpen Wiwat, and Phetrung Phanphiriya

Molecular ecological analysis on microbial community in biodegradation

process of organic solid waste 9 7

Shin Haruta, K. Nakamura, M. Ishii, and Y. lgarashi

JSPS Seminar II : Microbial Genetics and Microbial Diversity

in Southeast Asia 99

Endophytic fungi isolated from Ulmus davidiana and their association

with the host plant 101

Fusao Tomita, Michiko Tanaka, Teruo Sone, Michiyo Nara, Manabu Suto,

Atsushi Yokota, and Kozo Asano

Endophytic fungi prospecting from medicinal plants in Malaysia for useful

substances 102

Son Radu, Cheah Yoke Kqueen, Mohamed lsmail Abdul Karim, Manabu Suto,

Michiko Tanaka, and Fusao Tomita

The production of lepidimoide from polysaccharide extracted from

Abelmoschus sp. by endophytic fungi - Identification of product and the

producing organism 103

Kozo Asano, Michiko Tanaka, Makoto Yoshimura, Teruo Sone, Katsutoshi

Hirose, Manabu Suto, Atsushi Yokota and Fusao Tomita

Studies on Indonesian endophytic microorganism and their potentials

as a-glucosidase producer 104

Trisanti Anindyawati, Katsuichi Saito, Michiko Tanaka, Kozo Asano, Endang

Sukara, Hirokazu Matsui and Fusao Tomita

Isolation, optimization and characterization of xylanase from

endophytic fungi 105

Saisamorn Lumyong, Nakorn Norkaew, Damrong Ponputhachart and Fusao

Tomita

Isolation and partial purification of bacteriocin produced by microorganisms

from Agos-os 106

Julie D. Tan, Fusao Tornita, Kozo Asano and Flor Crisanta. F . Galvez

Development of 16s rDNA targeted probes for detection of some human

health beneficial strains of intestinal microorganisms 107

Akarat Suksomcheep, I Nengah Sujaya, Katsuichi Saito, Atushi Yokota, Kozo

Asano, and Fusao Tomita

Purification and characterization of protoporphyrinase from Pseudomonas

azelaica YA-1 108

Gina Rio Dedeles, Ayumi Abe, Katsuichi Saito, Teruo Sone, Kozo Asano, Koichi

Saitoh, Atsushi Yokota, and Fusao Tomita

Partial 16s rDNA analysis of anti-fungal producing streptomycetes

isolated from coastal areas of Thailand 109

Arinthip Thamchaipenet, Vachira Chaipackdee, Hiroko Kawasaki and Tatsuji

Seki

Identification of pathogenic and non-pathogenic Vibrio strains

from shrimp and shrimp farms in the Philippines using 16s rRNA

sequencing & biochemical ID systems 110

R. G. Monsalud, L. M. Tapay, F. 0. Magbanua, M. Kurahashi and A. Yokota

Isolation and identification of bacterial strains from marine mollusca 111

Midori Kurahashi and Akira Yokota

Inhibitory effect of an antagonistic streptomycete on the control of

Fusarium wilt pathogen of banana 112

Getha, K., Vikineswary, S. and Seki, T.

JSPS Seminar Ill : Development of Sago Industry 113

JSPSIII41 Sago palm, the high potential bioresource for green chemistry,

alternative to petrochemical industry 115

Ayaaki lshizaki

JSPSlll42 High-speed lactic acid production by electrodialysis culture-associated

product separation 116

Yoshiyuki Nomura

JSPSIIi-03 Direct fermentation of sago starch to lactic acid by a local Lactococcus

strain 117

Dulce M. Flores, Grashiela P. Silva, Jennifer T. Pena

Molecular characterization of a bacteriocin of Pediococcus acidilactici

and its production in sago and sugarcane-based substrates 118

F. B. Elegado, K. Sonomoto and A. lshizaki

Weed dynamics at pepper (Piper nigrum, L.) field under sago pith

residue and some mulches stress 119

H. M. H. Bintoro, Anis Tatik Maryani, Nobuo Sugiyama, Kuniyuki Saito

Economic and waste management of sago processing 120

K. 6. Bujang

In vitro culture of sago palm (Metroxylon sagu Rottb.) 121

Suravoot Yooyongwech, Krisda Wasantanarut, Sucharit Suanpairot, Pirojana

Luangpituksa, and Kanyaratt Supaibulwatana

JSPS Seminar IV : Development of Nitrogen-fixing Symbionts and

JSPSIV-I 1

Mycorrhizae for Sustainable Agriculture and Forestry 123

Expression of the engineered metallothionein gene in nodules of

Astragalus sinicus with promoters from the symbiotic plasmid 125

Rutchadaporn Sriprang, Makoto Hayashi, Mitsuo Yarnashita, Hisayo Ono,

Yoshikatsu Murooka

Development of rhizobial inoculant production and formulation:

sugar producing from various raw starch materials by usin

amylase-producing fungi 126

Panlada Tittabutr, Waraporn Payakapong, Neung Teaurnroong and Nantakorn

Boonkerd

Energy metabolism and host legume relationships in local isolates of

rhizobia; malic enzymes in rhizobia as a marker of the metabolism type 127

Shigeyuki Tajima, Suphawat Sinsuwongwat, Achara Nuntagij, Naofurni

Hirarnitsu and Mika Agarie

Cooperative degradation of simazine by plants and endophytes 128

Takashi Ozawa, Ryota Yoshida, Yoshiaki Wakashiro, and Hitorni Hase

Commercial production of biofertilizer and bioenhancer using Azospirillurn

and Bacillus spp. for improved growth of oil palm seedlings and bananas 129

Shamsuddin, 2. H., Arnir, H. G., Mia, M. A. B., Prernalatha, P., Halirni, M. S., Khor,

S. K., Marziah, M., Ariff, A. 6 . and Ooi, T. C.

Expression of the globin gene family responding to the microsymbionts infection

on Lotus japonicus 130

Toshiki Uchiumi, Yoshikazu Shirnoda, Tornoko Tsuruta, Yurni Mukoyoshi,

Akihiro Suzuki, Shusei Sato, Keishi Senoo, Satoshi Tabata, Shiro Higashi, and

Mikiko Abe

Study of Azospirillurn inoculant development for crops 131

Harmastini Sukiman, Sylvia Lekatornpessy, Dini Ariani and Ardiansyah

Nitrogen fixation of vegetable soybean in the farmers' fields of traditional

soybean growing area of northern Thailand and methods for improvement 132

Ampan Bhromsiri, Anongporn Yodnoi and Daniel Muloi

Different mechanisms of four aluminum (Al)-resistant transgenes for

Al toxicity in Arabidopsis thaliana 133

Bunichi Ezaki and Hideaki Matsurnoto

Residual effect of inoculated soybean in rotation system soybean-rice

cultivated in Kien Giang province, Vietnam 134

Nguyen Huu Hiep, Dang Thanh Son, Nguyen Van Bil, Pharn Cong Hoan and

Tran Trong Thang

Two ftsZ genes of Mesorhizobiurn huakuii 135

Makoto Fujie, Masatoshi Konishi, Takashi Yarnada

Glucose degradation by pure culture isolated microorganisms from the

anaerobic digestors of pineapple peel 151

Pornpan Lerstaveesin, Yuvadee Nakapadungrat, Saowanee Manatsirikiat,

Morakot Tanticharoen and Pawinee Chaiprasert

Study on acetic acid utilization of sulfate-reducing bacteria and methane-

producing bacteria at mesophilic temperature 152

Yuvadee Nakapadungrat, Pornpan Lerstaveesin, Ubonwan Chaiyo, Pawinee

Chaiprasert, Morakot Tanticharoen

Organic nitrogen removal from landfill leachate using an aerobic fixed film

bioreactor 153

R. B. C. Uy, W. I. Jose, W. L. Barraquio, and A. P. Rollon

Reduction in the transfer of nitrogen and phosphorus from paddy fields to a

nearby lake using a circular irrigation system 154

Ken-ichi Suga, Motoki Tatani, Jun-ichiro Tokunaga, Tornoko Fujirnura, Yoshio

Katakura, Takeshi Ornasa, Michirnasa Kishirnoto

JSPS Seminar VI : Bioactive Compounds from Bioresources Indigenous to

Southeast Asia 155

JSPSVIM Production of bioactive extracellular violet pigment, nostocine

A by freshwater cyanobacterium, Nostoc spongiaeforrne TlSTR 8169 157

Kazumasa Hirata, Susilaningsih Dwi, Sayaka Yoshitorni, and Kazuhisa

Miyarnoto

Biosynthetic study on rubber producing plants 158

Ei-ichiro Fukusaki and Akio Kobayashi

Production of sweet table wine by controlled alcohol fermentation using

immobilized yeast and an antimicrobial substance from paprika seeds 159

Tohru Okuda

Screening and identification of some endophyte microbes producing

Cinchona alkaloids 160

Partomuan Simanjuntak, Titi Parwati, Nia K. Sufiantini, Titik K. Prana, Judhi

Rachmat and Hirotaka Shibuya

Effect of crude extract from Hapalosiphon sp. TlSTR 8252

on beet armyworm 161

Aparat Mahakhant, Jirapatch Polchai, Watchareeporn Luakhurnhan, Prapaisri

Sornchai Vullapa Arunpairojana and Kazuhisa Miyarnoto

Identification of biosurfactant producing and oil degrading bacteria isolated

in Thailand by 16s rRNA gene-polymerase chain reaction technique 162

Attawut Impoolsup, Tornomi Uchida, Masahito Fukunaga, Mayurni Mizokarni,

Takashi Ishizu, Kazuyoshi Ohashi, Hirotaka Shibuya, lsamu Maeda, Kazuhisa

Miyarnoto

in wine-making \ Wimolsiri Porntaveewat, Tsutomu Takayanagi and KL

JSPS Seminar VII : Improvement of Enzyme Function with Prote

Engineering Technology

JSPSVII-01 Production of maltodextrin and glucose from cassava starch by enzyn

hydrolysis using am-I alpha-amylase and an glucoamylase 167

T.M. Espino, J.D. C. Jacob, F.Z. Tambalo and R.D. Tambalo

Thermostable protease production and strain improvement to overproduce

the enzymes of an indigenous thermophilic bacteria 168

N. R. Prayitno, A. M. Fuad, Ardiansyah and J. Rachmat

Thermostable lipase from locally isolated thermophilic bacterium 169

Raja Noor Zaliha Raja Abd. Rahman, Leow Thean Chor, Abu Bakar Salleh,

Che Nyonya Abd. Razak, Mahiran Basri and Mohd. Basyaruddin Abdul Rahman.

Effect of modification on Candida rugosa lipase: a structural approach 170

Salleh, A. B., Ario Tejo, B., Basri, M., Rahman, M. 5. A,, Rahman, R. N. Z. A.

Production and structural analyses of biosurfactant produced from

Pseudomonas sp. A41 using palm oil as carbon source 171

J. Thaniyavarn, A. Chongchin, N. Wanitsuksombut, S. Thaniyavarn, P.

Pinphanichakarn, A. Petsom, M. Morikawa and S. Kanaya.

Dextranase from Arthrobacter sp. AG-2 and characterization thereof 172

Supat Chareonpornwattana, Natinee Suvanasingha, Suhuttaya Jiranuntipon,

Anantapong Sukket, Jiraporn Thaniyavarn and Suthep Thaniyavarn

Subtilisin-like protease from a hyperthermophilic archaeon 173

Yuji Kannan, Yuichi Koga, Yohei Inoue, Mitsuru Haruki, Masahiro Takagi,

Tadayuki Imanaka, Masaaki Morikawa, and Shigenori Kanaya

JSPS Seminar Vlll : Process Development for Microbial Production of Organic

Acids, Bio-polymers and Related Food Additives 175

JAPSVIII41 Process development for microbial production of useful compounds from

regional resources in tropics 177

Suteaki Shioya, Hiroshi Shimizu, Keiji Uchiyama, Keisuke Nagahisa, Somchai

Krairak, Suchada Chanprateep, Songsri Kulpreecha, Arbakariya Bin AriR

Mohamed lsmail Abdul Karim, Suraini Abd. Aziz, Rosfarizan Mohamad, Somchai

Chauvatcharin, Patoomporn Chim-anage, Busaba Yongsmith

lnvestigation factors on effecting infectivity of NG.

exigua and Spodoptera litura larvae

Jaruwan Kongtarat

lnvestigation on the toxic metabolite production of Nomur;

Lepidopterous larvae

Pannipa Prompiboon

Studies on phathogenecity of local isolates of Nomuraea rilleyi agal,

Spodoptera litura 194

Phornpoj Srisukchayakul

Conidia production of the entomopathogenic fungus Nomuraea rileyi by solid

substrate fermentation 195

Thawatchai Mongkolwai, Ubon Vatanabot, Chumsri boonyat, Chanpen Wiwat

and Amaret Bhumiratana

Endophytic fungi of grasses from the central region of Thailand 196

Sansanalak Rachdawong and Wilairat Liewkongsataporn

Antagonistic effect of endophytic fungi on Colletotrichurn musae 197

Udomluck Thiantarworn, Saisamorn Lumyon, Wanchai Sonthichai and

Naowarat Cheeptham

Antifungal activity from endophytic fungi 198

Urat Pimolsri and Saisamorn Lumyong

Screening of marine photosynthetic bacteria producing 5-aminolevuliniv acid 199

Amornrat Tangprasittipap, Poonsuk Prasertsan and Wanna Choorit

Screening of phytase producing strains from Actinomycetes for application

in animal seed 200

Tewa Upathanpreecha, Saisamorn Lumyong and Chartchai Khanongnuch

Screening of ligninolytic enzyme from Basidiornycetes isolated in Thailand 201

Jittima Isarasenaruk, Yuwapin Dandusitapunth and Jones, E.B. G.

Isolation of Actinomycetes and screening for possible antitumor activities

from cave soil in Mae Hongson province 202

Jeerapun Jaiinphon, Saisamorn Lumyong, Wanchai Sonthichai and

Naowarat Cheeptham

Collection and isolation of lichen fungi for the Sioresources screening

programme 203

Veera Sri-indrasutdhi and Somsak Sivichai

Biodiversity study of freshwater fungi in 'Thailand 204

Somsak Sivichai and Nuttawut Boonyuen

Development of parasol mushroom (Macrolepiota) culture

for commercial scale 205

Chainarong Boonkemthong , Flegel T. W., Satit Thaithatgoon and Tearnworks

of Arunyik Mushroom Center

Isolation and identification of yeasts isolated from various natural

substrates in Thailand 206

Sasitorn Jindamorakot, Wanchern Potacharoen, Hiroko Kawasaki, Tatsuji Seki,

Manee Tantirungkit, Savitree Lirntong, Takashi Nakase and Morakot

Tunticharoen

Isolation and identification of coprophilous fungi 207

Sayanh Somrithipol, Nigel L. Hywel-Jones and Ruud Valyasevi

Marasmioid and Mycenoid Fungi in Thailand (phase I ) 208

Thitiya Boonpratuang, Porarnate Ruksawong, Timothy W. Flegel

Nigel Hywel-Jones and Dennis E. Desjardin

Isolation of fungi for bioactive compounds screening and lignin degrading

enzymes from Thai forests 209

Prasert Srikitikulchai, E.B. Gareth Jones, Sornsak Sivichai and Wanchern

Potacharoen

Where to classify Torpedospora arnbispinosa and T. radiata ? 210

Jariya Sakayaroj, K. Lai Pang and E. B. Gareth Jones

Alkaline-tolerant fungi in Thailand 21 1

Kladwang, W., Hywel-Jones, N., Lange, L, Bhurnirattana, A., Flegel, T., and

Meevuthisom, V.

Biodiversity of fungi on palms in sirindhorn peat swamp forest, Narathiwat,

Thailand 212

Aom Pinnoi, Urnpava Pinruan, Kevin D.Hyde and E.B.Gareth Jones

Microbial Genetics 21 3

P-MicroGenetic-01 Efficient expression of a heterologous protein in Escherichia coli

by phycocyanin promoter of Spirulina platensis 21 5

Chetkul, W. and Cheevadhanarak, S.

P-Miuu-Genetid2 Functional and cryptoregiochemical study of desC gene encoding A9

fatty acid desaturase from Spirulina platensis C1 (Althrospira sp. PCC 9438) 21 6

Meesapyodsuk, D, Reed, D. W., Cheevadhanarak, S., Deshniurn, P.,

Tanticharoen, M. and Covello, P. S.

P-Micro-Genetic-03 Unsaturated fatty acid defective mutant of Mucor rouxii : A useful tool for

studying the regulation of fatty acid synthesis 21 7

Rapeepun Pongchuachidthai, Kanchana Rueksorntawin, Kobkul Laoteng,

Morakot Tanticharoen and Supapon Cheevadhanarak

Transcriptional regulation of the A9-desaturase gene of Mucor rouxii

is mediated by a regulatory element with repressing activity 218

Rawisara Ruenwai, Kobkul Laoteng, Morakot Tanticharoen and Supapon

Cheevadhanarak

Development of a transformation system for Mucor rouxii ATCC 24905 by

electroporation of yeast-like cell 219

Sukanya Jeennor, Kanchana Rueksomtawin, Kobkul Laoteng, Morakot

Tanticharoen and Supapon Cheevadhanarak

Molecular cloning of hyaluronan synthase (HAS) gene from group C

Streptococcus zooepidemicus 220

Boonsri Jongsareejit, Amaret Bhumiratana, Masaaki Morikawa and Shigenori

Kanaya

Mutation of Arthrobacter sp. Strain AG-2 for enhancing dextranase

production 22 1

Suhuttaya Jiranuntipon, Suttiruk Tungjitpinitkarn, Supat Chareonpornwattana,

Jiraporn Thaniyavarn and Suthep Thaniyavarn.

Study of the effect of protease on recombinant protein poduction

in Aspergillus oryzae using GFP as a reporter 222

Anuwat Tachaleat, Somkiet Techkarnjanaruk and Supapon Cheevadhanarak

Effect of difference detergent and carbon sources on extracellular lipase

production by cloned Bacillus stearothermophilus TP811 in E.coli MI5

[ P R E P ~ ] 223

Padchanee Sangthong and Suree Phutrakul

Characteristics of Plutella xylostella trypsin and chymotrypsin genes

in relation to the use of their propeptides as pest control agents

Khemika Songjang, Barry N Perry and P D S Caligari

Improvement of methionine content in food and fodder yeast,

Saccharomyces cerevisiae, by genetic manipulation

Savitree Limtong, Ruthada Chankian, Chutima Disyen, Vichien

Kitpreechavanich, Manee Tantirungkij and Wichien Yongmanitchai

Strain improvement of starch-utilizing bacteria by mutagenesi

to enhance L-Lactic acid production 226

Sureelak Rodtong, Jitraporn Sansit, Phetchara Chimsoongnern, Kunthika

Vechklang and Ayaaki lshizaki

Detection and sequence analysis of the gene encoding L-lactate

dehydrogenase from starch-utilizing bacteria 227

Sureelak Rodtong

Cloning and expression of a chitinase gene from the Thai-isolated

Helicoverpa armigera nucleopolyhedrovirus 228

Navaporn Posayapisi, Kanokwan Poomputsa, Phenjun Mekvichitsaeng and

Somkiet Techkarnjnaruk

Development of expression vectors for propionibacteria and their

application to increase synthesis of 5-aminolevulinic acid. 229

P. Kiatpapan and Y. Murooka

Cloning of bt gene effecting Spodoptera exigua 230

Benjawan Lertwiriyawonga, Pattana Srifah and Jariya Chanpaisaengb

Conjugational transfer between E.coli and Streptomyces rimosus R7

and S. rimosus 401 8 231

Kanokon Yaodam, Nattika Pulsawat, Siriporn Khumwan

and Arinthip Thamchaipenet

C1 metal and cell-Xanthomonas fur mutants show alterated organic

peroxide resistance 232

R.Sallabhan, W. Eiamphungporn, S. Loprasertl, S.Atichartpongku1 and

S. Mongkolsuk

Modulation of oxidative stress responses by cadmium ion in Xanthomonas 233

Peerakarn Banjerdkij, Paiboon Vattanaviboon, Preeda Parkpian, Suvit

Loprasert and Skorn Mongkolsuk

Preliminary identification of fast-growing Bradyrhizobium japonicum

isolated from soybean root nodule in acid soil 234

Patima Permpoonpattana, Suwat Saengkerdsub and Kanjana Chansa-ngavej

Genetic structure of indigenous non-nodulating and nodulating populations

of Bradyrhizobium from Thailand soils 235

Neelawan Pongsilp, Neung Teaumroong, Atchara Nantagit, Michael J.

Sadowsky and Nantakon Boonkerd

Primer design for nodC gene of Bradyrhizobium 236

Wora-anong Mapakdee and Hataichanoke Niamsup

Identification of the accumulated intermediates from acenaphthylene

degradation by Rhizobium sp. CU-A1 transposon mutants 237

Siriwat Poonthrigpun, Amorn Petsom, Kanchana Juntongjin, Kobchai

Pattaragulwanit, Suthep Thaniyavarn and Pairoh Pinpanichakarn

Gene involving in acenaphthylene degradation of Rhizobium sp.

strain El I . 238

Tippawan Lorrattanachaiyong, Pairoh Pinpanichakarn, Kanchana Juntongjin,

Suthep Thaniyavarn and Kobchai Pattaragulwanit

Population Dynamics of Acetoclastic Methanogens and Methane

Production in Flooded Rice Root Soils 239

Sansanee Chawanakul, Pawinee Chaiprasert, Sirinthornthep Towprayoon,

Orapin Kerdchoechuen and Morakot Tanticharoen

A Study of methane production and classification of microorganisms

from root of rice cv. Supanburi 90 240

Winai Keawsawat, Jirasak Kongkiattikajorn and Somkiatti Tachakanchanarag

Phylogenetic Relationship of Paecilomyces Section lsarioideae based on

rDNA sequences 24 1

Janet Jennifer Luangsa-ard, Nigel Hywel-Jones, Leka Manoch and Robert A.

Samson

Desosamine transferase gene disruption in Streptomyces venezuelae :

a model for post-polyketide gene manipulation 242

Yuwadee Talawanich , Arinthip Thamchipenet, Prasat Kittakoop and Yongyuth

Yuthavong

Conference on Functional Genomics of Rice 243

PRicPOl Distribution of blast pathogen in Thailand related with pathogenicity on rice 245

Sermsiri Mayteeworakoon, Thanee Sriwongchai, Saengchai sripracone,

Suchada Pimpisitthavorn, Pilaiwan Hutamekalin and Pattama Sirithunya

PeiceU2 Fertility and mating type of Magnapothe grisea rice pathogen in Thailand 246

Saengchai Sriprakhon, Eduard Roumen, Arunee Surin,

Janet Jenifer Luangsa-ard, Thanapom Veerapraditsin,

Suchada Pimpisitthavorn and Pattama Sirithunya

AFLP analysis of blast pathogen diversity in Thailand 247

Pilaiwan Hutamekalin, Thanaporn Veerapraditsin, Suchada Pimpisitthavorn,

Thani Sreewongchai and Pattama Sirithunya

Studies inhibition of appressorium formation of Thai blast fungal 248

Suchada Pimpisiithavorn, Thanee Sreewongchai, Sermsiri Mayteeworakoon,

Pilaiwan Hutamekalin, Saengchai Sriprakhon, Kittipat Ukoskit and Pattama

Sirithunya

Detection of Rice Ragged Stunt Virus by RT-PCR method 249

T. Wijarat, S. Attathom, W. Kositrattana and 0. Chatcha wankarnphanich

Mapping multiple QTLs conferring brown planthopper resistance

using advance introgressed lines 250

J. Jairin, T. Toojinda, S. Tragoonrung, S. Amornsilpa, S. Sarkarung, S.

Wanchana, S. Tayapat and A. Vanavichit

Development of the particle inflow gun and optimizing the particle

bombardment method for efficient genetic transformation in mature

embryos of cereals 263

N. Abumhadi, A. Trifonova, S. Takumi, C. Nakamura, E. Todorovska, Ljubomir

Getov, N. Christov and A. Atanassov

llant Biotechnology 265

Plants as bioreactors for pharmaceutical protein production 267

Kazuhito Fujiyama and Tatsuji Seki

Isolation of replicase gene from papaya ring spot virus and transformation

of tobacco by Agrobacterium tumefaciens method 268

N.S. Kottearachchi, S. Kertbundit and M. Juricek

Transgenic papaya for resistance to papaya ring spot virus in Thailand 269

N. Warin, T.M. Burns, S. Chowpongpang, K. Kanokwaree, M. Boontum, M.

Sratoncjjun, S. Wasee, W. Kositratana, J. Dale and S. Attathom

Detection of two I-aminocyclopropane-Icarboxylate oxidase (ACO) genes

differential expression in developmental stages of papaya fruit using RT-

PCRmethod 270

Orawan Kumdee, Sermsiri Chanpreme and Parichart Burns

Detection of plant viruses by PCR without nucleic acid extraction 271

Supaporn Leamkhang, Lampung Raingwong, Nuchanard Warin

and Orawan Chatchawankanphanich

Sequence analysis of tomato-infecting geminiviruses in Thailand

Sopid Sawanwit, Supat Attathom and Orawan Chatchawankanphanich

lmmunoassays for detection of white fly-transmitted geminiviruses

in Thailand 273

C. Seepiban, N. Warin, R. Hongprayoon, S. Attathom and 0. Gajanandana

Transient GUS gene expression in Dendrobium by microprojectile

bombardment 274

Usa Ketsuwona, Paffana Srifah and Salak Phansiri

Mechanism of resistance to bacterial wilt disease caused by Ralstonia

solanacearum in pepper 275

Jutatape Watcharachaiyakup, Niphone Thaveechai, Julapark Chunwongse

and Wichai Kositratana

The application of protoplast technology for sugarcane breeding 276

Rongrong Homhual, Siwimol Juangjan and Prasert Chatwachirawong

The study of genetic relationship and molecular marker identification of SUT

Dendrocalamus asper 277

Kanok-orn Srilunchang and Mariena Ketudat-Cairns

Purification and characterization of U-mannosidases from Rosella (Hibiscus

sabdariffa L. var. sabdariffa) 278

Patjaraporn Wongvithoonyaporn, Dumrongkiet Arthan and Jisnuson Svasti

Bioactive Compounds from Bioresources 279

P-Bioactive Comp-01 Antimicrobial study on Thai medicinal plants against human

dermatopathogens 281

Mansuang Wuthi-udomlert and Sompop Prathanturarug

P-Bioactive Comp-02 Antimicrobial activity of the fruits of lllicium verum, Hook 282

Khesorn Nanthachit

P-Bioactive Comp-03 Antibacteria activity of the pods of Moringa oleifera, Lamk. 283

Khesorn Nanthachit

P-Bioactive Cornp-04 Antioxidant activity from Stevia (Stevia rebaudiana Bertoni) 284

Anchana Chanwitheesuk and Nuansri Rakariyatham

P-Bioactive Cornp-05 Bioactive secondary metabolites from endophytic fungi in Thailand 285

Palangpon Kongsaeree and Chanika Vongvien

P-Bioactive CompO6 Bio-prospecting of fungi in Thailand 286

Kanyawim Kirtikara, Chotika Suyarnsestakorn, Daraporn Pittayakhajonwut,

Jarin Kramyu, Kannawat Danvisetkanjana, Sasarose Rochanaruangrai and

Vanicha Vichai

P-~ioactive Comp-07 In vitro analysis of insect fungi: A cytotoxicity study 287

Supakit Wanasith, Napaporn Ittiworapong, Sukitaya Veeranondha, Nigel Hywel-

Jones and Patricia L. Watts

P-Bioactive Cornp-08 Establishing a facility to screen for substances with antituberculous drugs 288

Saovaluk vimuttipong, Kamolchanok Rukseree, Nuntawan Thongon, Prasit

Palittapongarnpim, Roongnapa Prachaktam, Charoen Chuchottaworn and

Poonpilas Hongmanee

P-Bioactive Cornp-09 Identification of a putative multifunctional nonribosomal peptide synthetase

gene of Xylaria sp. BCC 1067 with unusual motif of a condensation domain289

Porntip Paungmoung, Juntira Punya, Taweerat Vichisoonthonkul, Somchai

Pongpattanakitchote, Morakot Tanticharoen, Sakarindr Bhumiratana and

Supapon Cheevadhanarak

P-Bioactive Cornp-I o Generation of blocked mutants for studying the biosynthetic pathway of

polyketides in Xylaria sp. BCC1067 290

Somchai Pongpattanakitshote, Vasunan Chumchua, Juntira Punya and

Supapon Cheevadhanarak

m mpll Effect of ecdysteroid precursors feeding on formation of P-ecdysone in cell

suspension cultures of Vitex glabrata R.Br. 29 1

Preeyada Duanghaklang, Duangjai Sinlaparaya, Sanha Panichajakul and

Somsak Damronglert

ue ComplZ Optimum condition for extraction lnd~go from Indigofera tinctoria and

Baphicacanthus cusla and preparation of ready use natural indigo

for natural dyeing 292 P Nittaya Chanayath, Sorasak Lhieochaiphant and Suree Phutrakul

&a Cornpl3 Antibacterial activity of brown seaweed and phytoplankton extracts 293 F. Suprapa Tongsupa, Wilaiwan Chotigeat, Amornrat Phongdara I% and Kidchakan Supamattaya

a l Biotechnology 295

01 A Burkholderia pseudomallei oxyR : gene regulation and mutants b 9 characterization 297

1 S. Loprasert, R. Sallabhan, W. Whangsuk and S. Mongkolsuk

Subtypes, genotypes and "a" determinant variation of HBs gene in HBV in

Thailand 298

Apiradee Theamboonlers, Naruporn Scotraj, Maturapod Sriponthong,

Pojchanad Jantaradsamee and Yong Poovorawan

Subcellular localization of the adrenal secretory protease (ASP) expressed

in the Y1 cell line 299

Khomsorn Lomthaisong, Andrew Bicknell and Philip Lowry

Expression of the rat organic anion transporter gene OAT1 in the methylotrophic

yeast Pichia pastoris 300

Benjamas Punlungka, Chuenchit Boonchird and Samaisukh Sophasan

Monoclonal antibodies specific for Listeria 32 kDa heat-stable flagellar

antigens 30 1

Orawan Himananto and Patricia Watts

Sounthern blot analysis of 1291iNOS knockout and 1291wild type mice 302

Darunee Buripakdi Lawson, Nanapith Pratipnatalang, Wantanee Ratanasak

and Varaporn Akkarapatumwong

A new bio-compatible pH cleavable linker for solid phase synthesis of

squalamine analogues and transfection agents 303

Bordin Chitkul, Butrus Atrash, Stifun Mittoo and Mark Bradley

Directed evolution of malarial dihydrofolate reductase mutants conferring

resistance against potential lead compounds 304

Sudsanguan Chusacultanachai, Pornpan Thiensathit, Bongkot Tarnchompoo,

Worachart Sirawaraporn and Yongyuth Yuthavong

Heterologous expression and biochemical characterization of dihydrofolate

reductase (DHFR) and dihydrofolate reductase-thymidylate synthas

(DHFR-TS) from Plasmodium vivax 305

Ubolsree Leartsakulpanich, Mallika Imwong, Sasithon Pukriltayakamee,

Nicholas J. White, Snounou Georges, Worachart Sirawaraporn

and Yongyuth Yuthavong

Transcriptional upregulation of alternate cyclooxygenase isoform

in COX-1' and COX-2' cell lines

Vanicha Vichai and Kanyawim Kirtikara

Animal Biotechnology 307

P-hima14 I Pilot scale production of baculovirus in insect cell cuture for biopesticide 309

Wairuj Dechmahitkul ,Kathariya Paewkhaw, Kanokwan Pumputra, Utai Ketnuti

and Phenjun Mekvichitsaeng

P-AnimaW2 Expression of dengue viral envelope protein in Bombyx mori cells using

a baculovirus expression vector system 31 0

Amorn Owatworakit, Phenjun Mekvichitsaeng and Kanokwan Poomputsa

Optimization of recombinant dengue envelope protein production

in insect cell culture 31 1

Somsak Sotasan, Phenjun Mekvichitsaeng and Kanokwan Poomputsa

Process development in lactic acid bacteria production for probiotics use 312

Kanokarn Kocharin and Somchai Chauvatcharin

Digestive enzymes production from a soysauce koji mold for use as feed

supplement 31 3

Kwanjira Chantasartrassmee and Saovanee Dharmsthiti

Purification and production of thermo-acido-tolerant phytase

from Bacillus subtilis PHOI 314

Suttiluk Popanit and Saovanee Dharmsthiti

Feasibility of bacterial P -Mannanase utilization in quality improvement of

coconut meal diets fed for broilers 315

Chatchai Sanguansook, Saisamorn Lumyong, Suchon Tangtaweewipat

and Chartchai Khanongnuch

Applications of biotechnology in livestock production and fisheries

In Srilanka 316

C.M.B. Dematawewa

The biolarvicides bactivec and griselesf, as a new alternative,

in the control of Dengue, Malaria, Filariasis and Japanese

Encephalitis diseases vectors in Asia 317

Martinez Arias, A., Bounpone Sivadong, M. D. S. Rajamanthri, R. R. G.

Wimalaratne and M. R. S. S. Bandara.

Microbial population dynamics in anaerobic hybrid reactor during

start-up period 331

Chinnapong Wangnai, Pawinee Chaiprasert, Pratap Pullammanappallil

and Jurg Keller

Experience on improving tapioca starch wastewater treatment plant:

minimization of caustic soda consumption in UASB unit 332

Pratin Kullavanijaya, Pawinee Chaiprasert and Morakot Tanticharoen

Community composition of an acetate-enriched culture as revealed

by 16s rDNA clone library and fluorescent in situ hybridization 333

Somkiat Techkarnjanaruk, Wiwat Jupraputtasri, Pornpan Panichnumsin,

Annop Nopharatana, Supapon Cheevadhanarak, Pawinee Chaiprasert,

Sakarindr Bhumiratana and Morakot Tanticharoen

Improved sensitivity of fluorescent in situ hybridization for detection

of microbial communities by use of tyramide signal amplification system 334 Wiwat Jupraputtasri, Pornpan Panichnumsin, Annop Nopharatana,

Supapon Cheevadhanarak, Pawinee Chaiprasert, Sakarindr Bhumiratana,

Morakot Tanticharoen and Somkiet Techkamjnaruk i

Effect of sulfate on the performance of anaerobic CSTRs in treatment of I 1

modified starch wastewater 33

Pornpan Panichnumsin, Kalaya Charoensuksuwan, Annop Nopparatana,

Pawinee Chaiprasert, Morakot Tanticharoen and Sakarindr Bhumiratana

Nickel removal from aqueous solution by fungal biomass cultured

from starch wastewater 33

Pairat Kaewsarn

Thai strains of toluene-utilizing Acinetobacter calcoaceticus isolated from

contaminated water can degrade trichloroethylene (TCE) 33

Milintawisamai, N, S.Niamsanit, M. Ponglikitmongkol, Y. Choanahee, N.

Sripakdee, P. Sangchumpol, S.Bunsaup and M. Milintawisamai

Comparison between ammonia removal efficiencies of natural

and commercial nitrifying bacteria in saline wastewater

by sequencing batch reactor (SBR) 33

Chanyarat Paungfoo, Sompong GThomg, Satid Rokaerd, Poonsuk Prasertsan,

Nugul lntrasungkha and Rao Bhamidimarri

Integration of biofilter for water treatment in closed system

intensive shrimp culturing 33

Sunun Siriraksophon, Yingsak Kripinit, and Sahdev Singh

Bioemulsifier production from Acinetobacter calcoaceticus LP009 34

Wiyada Mongkolthanaruk and Saovanee Dharmsthiti

Treatment-15 Cornposting production from food waste using garbage automatic

decomposer extinguisher (GADE) bioreactors VII: Relevance of the bulking

p agent and system used to nitrogen in composting 34 1

! 7 Songpon Koonsrisook and Sudarut Tripetchkul

*-waste Treatment-16 Anaerobic degradability of distillery spentwash. 342

Pratin Kullavanijaya, Pawinee Chaiprasert and Morakot Tanticharoen

Oxygenase enzymes involved in degradation of p-hydroxyphenylacetate 343

Pimchai Chaiyen, Chutintorn Suadee and Kittisak Thodsaporn

MiP and laccase production from tropical endophytic xylaria 344

Chonticha Urairuj and Saisarnorn Lurnyong

Decolorization of reactive dyes by a microbial consortium 345

Nuttapun Supaka, Kanchana Juntongjin and Sornsak Damronglerd

Mechanism in decolorization of slop distillery waste by acetic acid bacteria 346

Arnpun Chaikulsareewath and Nongpanga Khunajakr

Enhancement of agricultural materials on microbial remediation of PAHs

contaminated soil 34 7

Narirat Charoenchang, Pairoh Pinpanichakarn, Kobchai Pattaragulwanit,

Suthep Thaniyavarn and Kanchana Juntongjin

The impact of application of chlorates in longan plantations on

the environment and the remedy 348

Ongprasert, Somchai, Sutikoolabud, Pathipan and Aurntong, Supathida

Microbial Waste utilization 349

P-Waste Utilization41 Fed-batch etanol production from waste cassava starch by pH-dependent

substrate feed 35 1

Theppanya Charoenrat, Penkhae Wanchaithanawong, Vichien

Kitpreechavanich and Wirat Vanichsriratana

P-Waste Utilition42 Utilization of Agro-Industrial wastes for the production of Monascus

red pigment by solid state fermentation 352

Keitkaew Apinhapat, Nayuree Chainton, Chiraporn Ananchaipatfana, Chutirna

Kittipinyovath, Sivawan Phoolphundh and Aporn Wongwicharn

P-Waste Util'ition43 Production of Lentinus edodes spawn in liquid media 353

Sornpreeda Bootsisai, Taweerat Vichitsoonthonkul and Penjun Makvichitsang

P-Waste UtilizationG Selection of bacterial strain which produced multienzyme complex

of cellulolytic and xylanolytic enzymes 354

Khin Lay Kyu, Patthra Pason and Khanok Ratanakhanokchai

P-Waste Util'ition45 Feather degradation by Bacillus sp. FK 46 in submerged cultivation 355

W. Suntornsuk and L. Suntornsuk