D I S H A A · Irish Wolfhound, English Springer Spaniel, Vizsla, Bernese Mountain Dog, Standard Poodle, Belgian Shepherd, Border Collie, Australian Shepherd, Border Terrier •hile

For more information, visit www.PurinaProPlanVets.com or call us at 1-800-222-VETS (8387),

8:00 am to 4:30 pm CST, Monday-Friday

PURINA TRADEMARKS ARE OWNED BY SOCIÉTÉ DES PRODUITS NESTLÉ S.A. ANY OTHER MARKS ARE PROPERTY OF THEIR RESPECTIVE OWNERS. PRINTED IN USA.

VET 0303A-0117 2016

Learn more at www.PurinaProPlanVets.com

STUDY DESIGN

Disorientation Interaction with people and/or

pets altered

Sleep/wake cycles altered

House soiling Activity altered (decline in activity,

restlessness, repetitive movements)

AnxietyD I S H A A

A concentrated source of MCT oil

When added to a dog’s daily diet, MCTs are easy to digest and are converted by the liver into an additional brain energy source, ketones, including βHB.

Ketones are used very efficiently by the brain of senior dogs, helping address the change in glucose metabolism

HOW MCT OIL NOURISHES THE BRAIN OF SENIOR DOGS

UNIQUE BLEND OF NUTRIENTS

Arginine Supports healthy circulation, blood

pressure and brain function

EPA + DHA Supports brain structure

and function. EPA helps reduce inflammation.

Antioxidants Help reduce oxidative stress

B Vitamins Used in energy metabolism and

DNA maintenance

CANINE COGNITIVE DYSFUNCTION SYNDROME

MECHANISM OF ACTION

INCIDENCE

Most commonly affects older dogs11

8–10 years: 3.4%10–12 years: 5%

12–14 years: 23.3%>14 years: 41%

RESULTS

EPA+DHA Antioxidants B Vitamins

BVITS

Arginine


BVITS

Arginine


BVITS

Arginine


BVITS

Arginine

MEDICAL INDICATIONS

In 90 days, dogs significantly improved across all DISHAA categories

• Randomized, double-blinded

• 3-month study of dogs fed adiet containing MCT oil and aunique blend of nutrients

• Dogs diagnosed by veterinarianwith CDS using DISHAA(DISHA + Anxiety)

CDS DEFINITION

Age-related neurodegenerative

disorder characterized by gradual and progressive

cognitive decline

An estimated14% of dogs 8+11

but only 1.9% are diagnosed

1 2016 Veterinary Medicine Landscape Dashboard2 Skerritt G. Canine Epilepsy. In Practice. 1988;10:27-30.3 Berendt M, Farquhar RG, Mandigers PJJ, et al. International veterinary epilepsy task force consensus report of epilepsy definition, classification and terminology in companion animals. BMC Vet Res 2015;11:182.

4 Packer RMA, Shihab NK, Torres BBJ, et al. Responses to Successive Anti-Epileptic Drugs in Canine Idiopathic Epilepsy. Vet Rec. 2015.

5 Arrol L, Penderis J, Garosi L, et al. Aetiology and Long-Term Outcome of Juvenile Epilepsy in 136 Dogs. Vet Rec.2012;170:335.

6 Podell M, Fenner W. Bromide Therapy in Refractory Canine Idiopathic Epilepsy. J Vet Intern Med. 1993;7:318-327.

7 Trepanier L, Schwark W, Van Schoick A, et al. Therapeutic Serum Drug Concentrations in Epileptic Dogs Treated with Potassium Bromide Alone or in Combination with Other Anticonvulsants: 122 cases (1992-1996). J Am Vet Med Assoc.1998;213:1449-1453.

8 Schwartz-Porsche D, Loscher W, Frey H. Therapeutic Efficacy of Phenobarbital and Primidone in Canine Epilepsy: A Comparison. J Vet Pharmacol Ther. 1985;8:113-119.

9 Law TH, Davies ESS, Pan Y, et al. A randomized trial of medium-chain TAG diet as treatment with idiopathic epilepsy. Brit J Nutr 2015;114:1438-1447.

10 Chang P-S, Augustin K, Boddum K, et al. Seizure Control by Decanoic Acid through Direct AMPA Receptor Inhibition. Brain. 2015;25:1-13.

11 Salvin HE, McGreevy PD, Sachdev PS, & Valenzuela MJ. Under diagnosis of canine cognitive dysfunction: a cross-sectional survey of older companion dogs. Vet J. 2010; 184: 277–81.

*

*as an adjunct to veterinary therapy

Idiopathic Epilepsy when fed as an adjunct to veterinary therapy

Cognitive Dysfunction Syndrome




VET 0303A-0117 2016


STUDY DESIGN


pets altered




AnxietyD I S H A A












DNA maintenance


MECHANISM OF ACTION

INCIDENCE


8–10 years: 3.4%10–12 years: 5%

12–14 years: 23.3%>14 years: 41%

RESULTS


BVITS

Arginine


BVITS

Arginine


BVITS

Arginine


BVITS

Arginine

MEDICAL INDICATIONS



• 3-month study of dogs fed adiet containing MCT oil and aunique blend of nutrients

• Dogs diagnosed by veterinarianwith CDS using DISHAA(DISHA + Anxiety)

CDS DEFINITION



cognitive decline









9 Law TH, Davies ESS, Pan Y, et al. A randomized trial of medium-chain TAG diet as treatment with idiopathic epilepsy. Brit J Nutr 2015;114:1438-1447.



*


Idiopathic Epilepsy when fed as an adjunct to veterinary therapy


• Randomized, placebo-controlled, double-blinded, crossover design

• 6-month study of dogs with idiopathic epilepsy; dogs fed a test diet with MCT oil for 3 months and control diet for 3 months

RESULTSMost dogs with idiopathic epilepsy showed a reduction in seizure frequency in 90 days when fed a test diet with MCT oil, as an adjunct to veterinary therapy.

• 21 dogs completed

• Dogs on medication but still having seizures (≥ 3 seizures in last 3 months)

OVER THE COURSE OF 90 DAYS:

71% of dogs showed a reduction in seizure frequency

48% of dogs showed a 50% or greater reduction in seizure frequency

14% of dogs achieved complete seizure freedom

STUDY DESIGN9

Cell Membrane

PostsynapticNeuronGluA1

MCT Oil

AMPAreceptor

MCTs block AMPA receptor

Synapse

PresynapticNeuron

X

Formulated with medium chain triglyceride oil to

help nutritionally manage dogs with epilepsy

+


BVITS

Arginine

Formulated with medium chain triglyceride oil to help nutritionally manage dogs with epilepsy

Enhanced with a unique blend of nutrients and medium chain triglyceride oil to help nutritionally manage dogs with


+


BVITS

Arginine



Cognitive Dysfunction Syndrome Enhanced with a unique

blend of nutrients and medium chain triglyceride

oil to help nutritionally manage dogs with Cognitive

Dysfunction Syndrome

CANINE EPILEPSY OVERVIEW

RESEARCH AND RESULTS

Most idiopathic seizures commence between

1 and 3 years of age2

• Labrador Retriever, Belgian Shepherd, Petit Basset Griffon Vendeen, Boxer, Irish Wolfhound, English Springer Spaniel, Vizsla, Bernese Mountain Dog, Standard Poodle, Belgian Shepherd, Border Collie, Australian Shepherd, Border Terrier

• While most prevalent, any breed/dog could be affected

Epilepsy is the #1 condition in dogs

diagnosed with neurological conditions1

• Typical antiepileptic drugs (phenobarbital and potassium bromide) are GABAergic enhancing inhibitory neurotransmission

• Experts believe MCTs (c-10 – decanoic acid) may have direct anti-seizure effects via blockage of AMPA receptors inhibiting excitatory neurotransmission; enhancing typical antiepileptic therapy10

PATENTP E N D I N G

A NOVEL MECHANISM OF ACTIONHEALTH BENEFITS

PURINA ® PRO PLAN ® VETERINARY DIETS NC NEUROCARE ™

An estimated

1 in 111 dogs are affected by epilepsy1

BENEFIT INGREDIENTS MECHANISM

NEUROLOGIC HEALTH• Helps nutritionally manage dogs

with idiopathic epilepsy (as an adjunct to veterinary therapy)

• Medium chain triglyceride oil (MCT oil)

• MCTs may have direct anti-seizure effects by being a noncompetitive AMPA receptor antagonist

COGNITIVE HEALTH• Helps nutritionally manage dogs with


• Medium chain triglyceride oil and a unique blend of nutrients

• MCTs are converted to β-hydroxybutyrate which can act as an alternative energy source to compensate for decreased glucose metabolism by the brain

• A unique blend of nutrients – arginine, EPA + DHA, antioxidants, and B-vitamins – to help support cognitive health

GENERAL HEALTH• Complete and balanced nutrition

formulated to meet the nutritional needs for growth of puppies and maintenance of adult dogs as established by AAFCO

• Antioxidant vitamins C & E

• Protein• Omega-3 fatty acids

• Contains antioxidant vitamins C and E to support a healthy immune system, including a high level of vitamin E to help reduce oxidative stress

• High in protein to help maintain lean muscle mass

• Omega-3 fatty acids to help support brain health and EPA to help reduce inflammation

BREEDS WITH GENETIC PREDISPOSITION TO EPILEPSY 3

Goal of Epilepsy Management

REDUCE OR ELIMINATE SEIZURE ACTIVITYReduction in seizure frequency of at least 50% is considered successful.4

Over two-thirds of dogs continue to have seizures long-term.5

20-30% of dogs remain poorly controlled despite appropriate medication. 6,7,8

• Randomized, placebo-controlled,double-blinded, crossover design

• 6-month study of dogs with idiopathic epilepsy;dogs fed a test diet with MCT oil for 3 monthsand control diet for 3 months



• Dogs on medication but still having seizures(≥ 3 seizures in last 3 months)


71% of dogs showed areduction in seizure frequency

48% of dogs showed a 50% or greater reduction in seizure frequency


STUDY DESIGN9

Cell Membrane


MCT Oil

AMPAreceptor


Synapse

PresynapticNeuron

X



+


BVITS

Arginine




+


BVITS

Arginine




Enhanced with a unique blend of nutrients and

medium chain triglyceride oil to help nutritionally

manage dogs with Cognitive Dysfunction Syndrome





• Labrador Retriever, Belgian Shepherd, Petit Basset Griffon Vendeen, Boxer,Irish Wolfhound, English Springer Spaniel, Vizsla, Bernese Mountain Dog,Standard Poodle, Border Collie, Australian Shepherd, Border Terrier




• Typical antiepileptic drugs(phenobarbital and potassiumbromide) are GABAergic enhancinginhibitory neurotransmission

• Experts believe MCTs (C10:0 –decanoic acid) may have directanti-seizure effects via blockage ofAMPA receptors inhibiting excitatoryneurotransmission; enhancingtypical antiepileptic therapy10

PATENTP E N D I N G



An estimated











• A unique blend of nutrients – arginine, EPA + DHA, antioxidants, and B-vitamins – to help support cognitive health













• Randomized, placebo-controlled,double-blinded, crossover design

• 6-month study of dogs with idiopathic epilepsy;dogs fed a test diet with MCT oil for 3 monthsand control diet for 3 months



• Dogs on medication but still having seizures(≥ 3 seizures in last 3 months)


71% of dogs showed areduction in seizure frequency

48% of dogs showed a 50%or greater reduction in seizure frequency


STUDY DESIGN9

Cell Membrane


MCT Oil

AMPAreceptor


Synapse

PresynapticNeuron

X



+


BVITS

Arginine




+


BVITS

Arginine




Enhanced with a unique blend of nutrients and

medium chain triglyceride oil to help nutritionally

manage dogs with Cognitive Dysfunction Syndrome





• Labrador Retriever, Belgian Shepherd, Petit Basset Griffon Vendeen, Boxer,Irish Wolfhound, English Springer Spaniel, Vizsla, Bernese Mountain Dog,Standard Poodle, Belgian Shepherd, Border Collie, Australian Shepherd,Border Terrier




• Typical antiepileptic drugs(phenobarbital and potassiumbromide) are GABAergic enhancinginhibitory neurotransmission

• Experts believe MCTs (C10:0 –decanoic acid) may have directanti-seizure effects via blockage ofAMPA receptors inhibiting excitatoryneurotransmission; enhancingtypical antiepileptic therapy10

PATENTP E N D I N G



An estimated











• A unique blend of nutrients – arginine, EPA + DHA, antioxidants, and B-vitamins – tohelp support cognitive health
















VET 0303A-0117 2016


STUDY DESIGN


pets altered




AnxietyD I S H A A












DNA maintenance


MECHANISM OF ACTION

INCIDENCE


8–10 years: 3.4%10–12 years: 5%

12–14 years: 23.3%>14 years: 41%

RESULTS


BVITS

Arginine


BVITS

Arginine


BVITS

Arginine


BVITS

Arginine

MEDICAL INDICATIONS



• 3-month study of dogs fed a diet containing MCT oil and a unique blend of nutrients

• Dogs diagnosed with CDS by veterinarian using DISHAA (DISHA + Anxiety)

CDS DEFINITION



cognitive decline









9 Law TH, Davies ES, Pan Y, et al. A randomised trial of a medium-chain TAG diet as treatment for dogs with idiopathic epilepsy. Br J Nutr. 2015 Nov 14;114(9):1438-47



*


Idiopathic Epilepsy as an adjunct to veterinary therapy


Documents

D I S H A A · Irish Wolfhound, English Springer Spaniel, Vizsla, Bernese Mountain Dog, Standard Poodle, Belgian Shepherd, Border Collie, Australian Shepherd, Border Terrier •hile