- Home
Documents
- de DNA.pdf · todo de la lisis alcalina (4) y se marcó radioactivamente con 32P (dCTP) (Amersham)...
5
de DNA.pdf · todo de la lisis alcalina (4) y se marcó radioactivamente con 32P (dCTP) (Amersham) por el método de «nicktranslation» (5)
Embed Size (px)
Citation preview
![Structure of the Mouse CD72 (Lyb-2) Gene and Alternatively ...med.stanford.edu/content/dam/sm/robinsonlab/documents/ying_j_im… · (C57L) probe labeled with [~x-~’P] dCTP (Amersham,](https://img.pdfslide.net/doc/110x75/5f09efb77e708231d42937bc/structure-of-the-mouse-cd72-lyb-2-gene-and-alternatively-med-c57l-probe.jpg)
![Cloning genefragmentcodingfor a · sert [800 base pairs (bp)] was nick-translated with [a-32p]-dCTP(800Ci/mmol;1 Ci =37GBq)usinganick-translation kit (Amersham). Hybridization wasconducted](https://img.pdfslide.net/doc/110x75/61246ffe355dae44837fbe94/cloning-genefragmentcodingfor-a-sert-800-base-pairs-bp-was-nick-translated-with.jpg)
![Molecular cloning mRNA - PNASprobe wasthe EcoRI-cut gel-purified OATasecDNAinsert labeledwith[32P]dCTP(Amersham)bynick-translation (31). The hybridized blot was washed at high stringency](https://img.pdfslide.net/doc/110x75/5e3ccfe57efb5544245a1e66/molecular-cloning-mrna-pnas-probe-wasthe-ecori-cut-gel-purified-oatasecdnainsert.jpg)
