DHI-The is for

INFORMATION

i

Mission Statement “To serve National DHIA members and the dairy industry

in advancing dairy information services.”

Vision Statement National DHIA will lead the dairy information industry to create,

utilize and advance world class information resources that are accurate, credible and uniform. 

We will accomplish this by:- Ensuring information accuracy

- Representing and supporting our members’ interests- Being the direct voice for the dairy information industry

- Working with industry partners

Polymorphism“poly” = many “morph” = form

Generalpopulation 94%

6%Single nucleotidePolymorphism (SNP)

GG GT TT

Genotype

Phenotype

Match Made In Heaven

Consider the possibility…4.5 million

cows

coast to coastCanada to

Mexico

Network of laboratories

Surveillance

Production efficiency

Genomics

Animal Health

Product QualityInternational

Competitiveness

Sustainability

to enhance:

to improve:

How Much Information is in Milk?

Everything– Cow– Environment

metabolitesantibodies

DNA

antig

ens

Caseins(A1/A2)

Fatty Acids(CLA)

Altering the Nutritional Value of Milk

An optimal milk exists– Phenotype/Genotype

=?

Mastitis-Udder InfectionIn early stages of infection,

there’s communication going on

Discover the language!

Johne’s Disease

Could milk work?

Johne’s

• Mycobacterium avium subspecies paratuberculosis(MAP)

Robert Whitlock, University of Pennsylvania

Won First Place in County Fair - Two Months Prior

Johne’s

Johne’s

• 22% increase in total sample volume from 2006

2007 Commercial Lab Activity

0

50

100

150

200

2003 2004 2005 2006 2007

Sam

ple

Vo

lum

e (0

00's

)

Milk Johne’s Testing

Milk Johne’s Testing Program

• Very Important Development– Links DHI to Animal

Health Programs– Showcases DHI

Infrastructure

TB

Brucellosis

Salmonella

• National Voluntary Program– Looking to DHI as

industry leader

Milk Johne’s Testing Program

• Advantages for Voluntary Program– Sample’s already in labs– Program built into dairy software– Linked with performance data

$

Herd Management Report

Milk Production 305-d ME (lbs) 1st lactation 2nd lactation 3rd lactation Johne’s Negative Group 21,537 21,763 19,210 Johne’s Positive Group 19,723 20,104 17,712 DIFFERENCE 1,814 1,659 1,498 Component Value 305-d ME (lbs) Protein Fat Johne’s Negative Group 36 40 Johne’s Positive Group -18 -28 DIFFERENCE 54 68

Johne’s Disease

Could milk work?

Leukosis

Leukosis

BLV – Bovine Leukosis Virus; Enzoonotic Bovine Leukosis

Leukosis

NAHMS ‘96

Leukosis

Leukosis

Leukosis Milk Testing Program

It’s the information!

Johne’s Disease

Could milk work?

BVD

Leukosis Disease

BVD

• Bovine Viral Diarrhea

BVD

BVDBVD

BVD Milk Testing Program

Traditional Program– In line samples – Ear notch

BVD Milk Testing Program

• 1000-Head Herd ($150 pooling fee)

– 4 PCR X $40 = $160

– 5 PCR X $40 = $200

– 50 ELISA X $6 = $300

– Total $810/1000 = $0.81/Cow ($0.50-$2.50)

250 Samples 250 Samples 250 Samples

50 Samples 50 Samples 50 Samples 50 Samples

50 Samples

250 Samples

50 Samples

PI Cow

Johne’s Disease

Could milk work?

Progesterone

Leukosis DiseaseBVD

Figure 1. The estrus cycle near the time of ovulation.

Progesterone Testing

• Progesterone ELISA– Individual animal test– Quantitative ELISA– High/low • Evaluation of

Synchronization– Progesterone from

functional CL– CL required to

respond to synchronization

Progesterone TestingProgesterone in Bovine Serum Versus Milk

0.0

2.0

4.0

6.0

8.0

10.0

12.0

14.0

16.0

18.0

Open < 14 Open 35-60 61-80 81-100 101-120 121-140 141-160 161+

Reproductive Status

P4

(n

g/m

L)

Milk

Serum

Progesterone Testing

Synchronization Program Evaluation

2 days

PGF2α PGF2αPGF2α GnRH GnRH AI

12 h14 days 14 days 7 days

HIGHHIGH HIGH HIGH LOW

Desired Progesterone Levels

Figure 2. PreSynch + OvSynch

Progesterone ELISA

• When Things Are Working

Progesterone Analysis Of Synchronization

0

10

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50

60

70

80

90

100

Lut 1 Lut 2 Lut 3

Lutalyse Shot Sequence

Pe

rce

nta

ge

Lo

w P

rog

es

tero

ne

1st Lactation

2nd+ Lactation

TARGET

Progesterone ELISA

• When Things Are Not Working

Progesterone Analysis Of Synchronization

0

10

20

30

40

50

60

70

80

90

100

Lut 1 Lut 2 Lut 3

Lutalyse Shot Sequence

Perc

en

tag

e L

ow

Pro

geste

ron

e Heifers

Cows

TARGET

Proposed Progesterone Profiling• Identify and Test Critical Samples• Monitor % Non-compliance

Progesterone Non-Compliance

0

10

20

30

40

50

60

70

1/12

/200

6

1/26

/200

6

2/9/

2006

2/23

/200

6

3/9/

2006

3/23

/200

6

4/6/

2006

4/20

/200

6

5/4/

2006

5/18

/200

6

6/1/

2006

6/15

/200

6

6/29

/200

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7/13

/200

6

7/27

/200

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/200

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8/24

/200

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9/7/

2006

9/21

/200

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10/5

/200

6

10/1

9/20

06

11/2

/200

6

11/1

6/20

06

11/3

0/20

06

12/1

4/20

06

Test Date

Per

cen

t N

on

-Co

mp

lian

ce

1st Lactation

2nd Lactation

3rd+ Lactation

BVD

Johne’s Disease

Could milk work?

Leukosis DiseaseProgesterone

Looking for Information?It’s In Her Milk

• Significant diagnostic opportunity• Immediate effects on management• Future effects on genetic selection• Enhance value of animal recording