Canine distemper virus (Distemper-1) ANTISERUM: Custom Monoclonals International. [email protected] Mouse monoclonal antibody. Clone: DVD-12. Isotype: IgG2Bk REACTIVITY ACCORDING TO MANUFACTURER: Immunofluorescence: It detects mainly cytoplasmic inclusion bodies. Immunohistochemistry: Effective on paraffin-embedded tissues. WORKING DILUTION: 1/800. Pretreatment with steamer (citrate buffer, pH 6.0). METHOD: LSAB2-PO. 30 min. RT. CELLS/TISSUES STAINED (CANINE): Kidney: Strong and diffuse reaction in urothelium of renal pelvis. Inclusion bodies are not as apparent as with HE stain. Urinary bladder: Similar reaction in urothelium. REFERENCES: Canine distemper (Distemper-2) ANTISERUM: VMRD (CDV-NP). Mouse monoclonal antibody. Isotype: IgG2bk>IgG1k REACTIVITY ACCORDING TO MANUFACTURER: Specific for canine distemper virus. It does not react with canine adenovirus type 2, canine coronavirus, canine parainfluenza virus or canine parvovirus. By immunofluorescence, staining is only cytoplasmic; inclusion bodies are stained most intensely. It is suitable also for immunohistochemistry using paraffin-embedded tissues. STAINING PROCEDURE ACCORDING TO MANUFACTURER: WORKING DILUTION: 1/800. Pretreatment with steamer (citrate buffer, pH 6.0). METHOD: LSAB 2-PO. 30 min. RT. CELLS/TISSUES STAINED (canine tissues unless specified): Urinary bladder: Diffuse cytoplasmic staining and occasional nuclear inclusions in urothelium. Similar in urothelium of renal pelvis. Epithelial cells of airways in the lung and macrophages in pulmonary parenchyma. Lymphoid cells in the spleen. REFERENCES:

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canine distemper 2015

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Canine distemper virus (Distemper-1) ANTISERUM: Custom Monoclonals International. [email protected] Mouse monoclonal antibody. Clone: DVD-12. Isotype: IgG2Bk REACTIVITY ACCORDING TO MANUFACTURER: Immunofluorescence: It detects mainly cytoplasmic inclusion bodies. Immunohistochemistry: Effective on paraffin-embedded tissues. WORKING DILUTION: 1/800. Pretreatment with steamer (citrate buffer, pH 6.0). METHOD: LSAB2-PO. 30 min. RT. CELLS/TISSUES STAINED (CANINE): Kidney: Strong and diffuse reaction in urothelium of renal pelvis. Inclusion bodies are not as apparent as with HE stain. Urinary bladder: Similar reaction in urothelium. REFERENCES:

Canine distemper (Distemper-2) ANTISERUM: VMRD (CDV-NP). Mouse monoclonal antibody. Isotype: IgG2bk>IgG1k REACTIVITY ACCORDING TO MANUFACTURER: Specific for canine distemper virus. It does not react with canine adenovirus type 2, canine coronavirus, canine parainfluenza virus or canine parvovirus. By immunofluorescence, staining is only cytoplasmic; inclusion bodies are stained most intensely. It is suitable also for immunohistochemistry using paraffin-embedded tissues. STAINING PROCEDURE ACCORDING TO MANUFACTURER: WORKING DILUTION: 1/800. Pretreatment with steamer (citrate buffer, pH 6.0). METHOD: LSAB 2-PO. 30 min. RT. CELLS/TISSUES STAINED (canine tissues unless specified): Urinary bladder: Diffuse cytoplasmic staining and occasional nuclear inclusions in urothelium. Similar in urothelium of renal pelvis. Epithelial cells of airways in the lung and macrophages in pulmonary parenchyma. Lymphoid cells in the spleen. REFERENCES: