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Dr. Anna Colangeli
Chair of Biology: Camosun College
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Genetic Research in the Garden 1
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**Based on phenotypic characteristics*Natural
Selection*Artificial Selection*Asexual
Reproduction*Cloning*grafting
Genetic Research in the Garden 2
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Genetic Research in the Garden 3
**Selecting best plants*Propagating by open pollination*Creating
hybrids by controlled crosses*Stabilizing hybrid lines by
replanting*Preserve original stock to maintain diversity*Preserving
heritage/heirloom varieties
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Genetic Research in the Garden 4
**Urban gardeners*Seed savers*Community gardening networks*Small
Agricultural Seed producers*Many others…..
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Genetic Research in the Garden 5
brings gardeners and farmers into the seed conversation by
offering a small-scale seed trial that can be done in a small space
and with a low time commitment.
http://www.bcseedtrials.ca/
http://www.bcseedtrials.ca/
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**Genetic engineering…or more accurately
*Recombinant DNA technology*Why is this possible?*Because every
organism on the planet uses the same genetic code
Genetic Research in the Garden 6
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**Precise cutting tools*Restriction enzymes*CRISPR/CAS9
*Rapid amplification of specific gene(s)*PCR – polymerase chain
reaction
* A natural genetic engineer*Agrobacterium tumefaciens
Genetic Research in the Garden 7
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Genetic Research in the Garden 8
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Genetic Research in the Garden 9
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Ti PlasmidpLUX Plasmid
Replace tumor and opine genes with genes of interest
Genetic Research in the Garden 10
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Genetic Research in the Garden 11
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Glow in the
dark plants!
pLUX Plasmid
Genetic Research in the Garden 12
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Genetic Research in the Garden 13
**GMO seeds are currently not available to home gardener
*Will probably not play a major role
http://www.innovationessence.com/first-genetically-modified/http://modernfarmer.com/2015/03/fda-approves-gmo-apples-and-potatoes/
http://www.innovationessence.com/first-genetically-modified/http://modernfarmer.com/2015/03/fda-approves-gmo-apples-and-potatoes/
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Genetic Research in the Garden 14
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Genetic Research in the Garden 15
www.isaaa.org
http://www.isaaa.org/
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Genetic Research in the Garden 16
http://www.hc-sc.gc.ca/fn-an/gmf-agm/appro/index-eng.php
http://www.hc-sc.gc.ca/fn-an/gmf-agm/appro/index-eng.php
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Genetic Research in the Garden 17
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*FasTrack*SMART Breeding
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Genetic Research in the Garden 18
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* http://www.goodfruit.com/on-a-fastrack/
https://agresearchmag.ars.usda.gov/2011/mar/breeding
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Genetic Research in the Garden 19
* http://ucanr.edu/sites/fastrack/Approach/
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Genetic Research in the Garden 20
* http://ucanr.edu/sites/fastrack/Approach/
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Genetic Research in the Garden 21
http://ucanr.edu/sites/fastrack/Approach/
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Genetic Research in the Garden 22
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http://www.greenpeace.org/international/en/publications/Campaign-reports/Agriculture/Smart-Breeding/
http://www.greenpeace.org/international/en/publications/Campaign-reports/Agriculture/Smart-Breeding/
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Genetic Research in the Garden 23
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*Selection with Markers and Advanced Reproductive Technology
*Utilizes MAS
*Marker Assisted Selection
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Genetic Research in the Garden 24
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(http://barleyworld.org/oregonwolfe).
http://barleyworld.org/oregonwolfe
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Genetic Research in the Garden 25
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https://www.washingtonpost.com/apps/g/page/national/screening-genes-for-better-breeding/870/
https://www.washingtonpost.com/apps/g/page/national/screening-genes-for-better-breeding/870/
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Genetic Research in the Garden 26
http://www.greenpeace.org/eu-unit/Global/eu-unit/reports-briefings/2014/Smart%20breeding%20-%20The%20next%20generation.pdf
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Genetic Research in the Garden
27http://www.greenpeace.org/eu-unit/Global/eu-unit/reports-briefings/2014/Smart%20breeding%20-%20The%20next%20generation.pdf
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Genetic Research in the Garden 28
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http://www.greenpeace.org/international/en/publications/Campaign-reports/Agriculture/Smart-Breeding/
http://www.greenpeace.org/international/en/publications/Campaign-reports/Agriculture/Smart-Breeding/
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Genetic Research in the Garden 29
**Will they be high tech????
http://www.nytimes.com/2013/04/25/technology/personaltech/calling-on-gadgetry-to-keep-the-garden-growing.html
http://www.nytimes.com/2013/04/25/technology/personaltech/calling-on-gadgetry-to-keep-the-garden-growing.html
