Draft National 304(a) Aluminum Aquatic Life Criteria

Diana EignorEcological Risk Assessment Branch

Health and Ecological Criteria DivisionOffice of Science and Technology

Office of Water US Environmental Protection Agency

bull Attendees who are members of the media can submit any questions to the press office Trisha Lynn (202) 564-2615

2

Overview

Background 2017 Recommended Criteria Status

3

Sources of Aluminumbull Aluminum is found in most rocks and

minerals and can enter the environment through natural weathering

bull Aluminumrsquos solubility increase under acidic (pH lt6) or basic (pH gt8) water conditions

bull Aluminum is actively mined from bauxite used in industrial processes and alum (potassium aluminum sulfate) is used in the clarification process at drinking water facilities 4

Current 1988 Aluminum Criteriabull The current 1988 criteria account for the

ndash Influence of pH on toxicity (solely by limiting criteria application range)

ndash Similarity of acute toxicity of fish and invertebrates

ndash The greater sensitivity of invertebrates in chronic toxicity tests

5

Current 1988 Aluminum Criteriabull The final 1988 criteria document recommended

ndash An acute criterion of 750 microgL total aluminum when the pH is between 65 and 90

ndash Chronic criterion of 87 microgL total aluminum when the pH is between 65 and 90

ndash The 1988 freshwater chronic criterion of 87 microgL was lowered based on two tests (brook trout and striped bass)

ndash The studies were not pH adjusted and all the data were not normalized for hardness The 1988 existing criteria did not take hardness and DOC into account

6

Aluminum Standardsbull Aluminum Standards

ndash About 20 states have numeric criteria for aluminum for aquatic life

bull Most are based on EPArsquos 1988 values and two are hardness-based equations (CO and NM)

bull There are numerous site-specific criteria that involve the Biotic Ligand Model (BLM) or a Water Effects Ratio (WER) -adjusted 1988 aluminum criteria

ndash Aluminum is not a priority pollutant

7

Criteria Development Processbull All EPA Aquatic Life Criteria for Toxics undergo rigorous

scientific development and review processbull Develop criteria document draftbull Intra-agency peer review process bull Independent external peer reviewbull Revisions based on external peer review

Intra-agency review of draft criteria documentbull Release to the public in draft form to obtain scientific views

Revisions considering public comments Intra-agency review of final criteria document

bull Publication of final criteria document bull States may then consider recommended criteria in

development and adoption of state water quality standards8

Framework for Updated Draft Aluminum Criteria

I Screen toxicological studies for high

quality data

II Consider water conditions effects on

aluminum bioavailability

III Normalize toxicity data in species

sensitivity distribution to a set of water quality conditions

IV Construct sensitivity distribution to determine criteria

at set conditions

9

I Screen toxicological studies for high quality data

bull First step in all criteria developmentndash Data are evaluated based on the data quality objectives

outlined in the 1985 Guidelines methodology and other toxicity testing recommendations (ie EPA ASTM)

ndash High quality data are used quantitatively to develop criteria magnitudes

ndash Lower quality data are used qualitatively if appropriatendash Lowest quality data are considered unacceptable and not

usedbull Data are grouped into acute and chronic exposures

10

Aluminum Toxicity Databull The updated freshwater aluminum criteria meets the

acute and chronic minimum data requirements outlined in EPArsquos 1985 Guidelines

InvertebrateSpecies

Fish Species

AmphibianSpecies

Total Genera

Acute1988 AWQC 8 7 - 142017 Draft AWQC 11 8 1 18

Chronic1988 AWQC 2 1 - 32017 Draft AWQC 7 4 - 11

11 1988 AWQC Chronic CCC based on two studies for the striped

bass and brook trout

Aluminum Toxicity Data Freshwater mussels have been

suggested to be potentially sensitive to aluminum

EPA worked collaboratively with USGSrsquos Columbia Environmental Research Center (CERC) to have acute and chronic aluminum toxicity tests conducted with a unionid mussel (fatmucket Lampsilis siliquoidea) in water-only exposures to obtain additional data on invertebrates New data on amphipod (Hyalella azteca)

were collected to confirm laboratory conditions

12

Aluminum Toxicity Data

13

bull New data and models examinedndash Data submitted to EU REACH program

bull New freshwater aluminum toxicity data generated by Oregon State University

bull 2016 BLM model provided by the European Aluminum Association

ndash Saltwater data provided by Australia bull Insufficient data to develop estuarinemarine acute or chronic

criteria at this timendash Multiple Linear Regression (MLR) model developed by

DeForest et al 2017bull DeForest DK KV Brix LM Tear and WJ Adams 2017 Multiple Linear Regression (MLR) models for predicting chronic aluminum

toxicity to freshwater aquatic organisms and developing water quality guidelines Environ Toxicol Chem (DOI101002etc3922)

14

Minimum Dataset For Freshwater Criteria Derivation

SALMONID SECONDFISHFAMILY

CHORDATA

PLANKTONICCRUSTACEAN

BENTHIC CRUSTACEAN

INSECT ROTIFERA ANNELIDA MOLLUSCA

OTHERINSECT ORMOLLUSCA

EstuarineMarine Minimum Data Requirements

15

Family Minimum Data Requirement EstuarineMarine

Acute(Phylum Family Genus)

Chronic(Phylum Family Genus)

Family in the phylum Chordata - -

Family in the phylum Chordata - -

Either the Mysidae or Penaeidae family - -

Family in a phylum other than Arthropoda or Chordata Mollusca Ostreidae Crassostrea -

Family in a phylum other than Chordata Annelida Nereididae Neanthes -

Family in a phylum other than Chordata Annelida Capitellidae Capitella -

Family in a phylum other than Chordata Annelida Ctenodrilidae Ctenodrilus -

Any other family Arthropoda Ameiridae Nitokra -

II Evaluate water quality conditions to determine applicability

bull Criteria are often based on one or more water quality conditions (eg hardness) that affect toxicity of the material to test organisms

bull Aluminum toxicity is highly dependent on the pH of the test waterndash The pH of test waters affects the amount and form

of aluminum that is bioavailable to test speciesndash Hardness and DOC (Dissolved Organic Carbon)

also influence the amount of aluminum that is bioavailable

16

Multiple Bioavailability Modeling Approaches Underwent External Peer Review

17

bull Biotic Ligand Modelsbull Full aluminum BLM (2016 model version) ndash Temperature pH

Dissolved organic carbon (DOC) Major cations (calcium [Ca] magnesium [Mg] sodium [Na] and potassium [K]) Major anions (sulfate [SO4] and chloride [Cl]) Alkalinity and Sulfide

bull Simplified aluminum BLM - pH hardness DOC temperature

bull Multilinear Regression Equationsbull pH and Hardness

Accounting for Effects of Water Chemistry on Bioavailability and Toxicity

bull Water quality criteria can be modified quantitatively when enough data are available to demonstrate that those conditions similarly affect the results with a variety of speciesndash High quality data in the draft aluminum document demonstrated

that pH DOC and hardness affected a variety of species in a similar way when exposed to aluminum concentrations

bull Recent publications by Cardwell et al (2017) and Gensemer et al (2017) summarized short-term aluminum chronic toxicity data across a range of DOC pH and hardness values for three species

18

Multiple Linear Regression (MLR) Models to Account for Effects of Water Chemistry

bull MLR models were developed for each species (DeForest et al 2017)ndash 119862119862 119889119889119906119906119887119887119894119894119886119886 11986411986411986211986220=119890119890[minus41026+[0525timesln(119863119863119874119874119862119862)]+[2201timesln(ℎ119886119886119903119903119889119889)]+(11282times119901119901119867119867)minus(0633times1199011199011198671198672)minus[0264times119901119901119867119867ln(ℎ119886119886119903119903119889119889)]]

ndash 119875119875 119901119901119903119903119900119900119898119898119890119890119897119897119886119886119904119904 11986411986411986211986220=119890119890[minus14029+[0503timesln(119863119863119874119874119862119862)]+[3443timesln(ℎ119886119886119903119903119889119889)]+(3131times119901119901119867119867)minus[0494times119901119901119867119867ln(ℎ119886119886119903119903119889119889)]]

bull These models were applied to both the acute and chronic aluminum toxicity datandash The C dubia model was applied to invertebrate datandash The P promelas model was applied to vertebrate data

19

III Normalize toxicity data in species sensitivity distribution to a set of water

quality conditionsbull Just like single parameter hardness models the MLR

models were used to normalize the aluminum toxicity data to a single set of water quality conditionsndash As an illustration for one set of calculations the draft

aluminum AWQC set these conditions to pH 7 hardness of 100 mgL and DOC of 1 mgL

bull This allows for comparisons to be made between species based on their sensitivity to aluminumndash Apples to apples

20

IV Construct sensitivity distribution to determine criteria at set conditions

21

Figure 8 Ranked Summary of Total Aluminum Genus Mean Acute Values (GMAVs) - Freshwater at pH 7 Hardness of 100 mgL and DOC of 1 mgL

IV Construct sensitivity distribution to determine criteria at set conditions

22

Figure 10 Ranked Summary of Total Aluminum Genus Mean Chronic Values (GMCVs) ndash Freshwater Supplemented with Other Data to Fulfill Missing MDRs at pH 7 Hardness of 100 mgL and DOC of 1 mgL

Aluminum Criteria Calculatorbull The relative ranking of genera in the sensitivity

distribution to which the toxicity data are normalized will change based on the set of water chemistry conditions (ie pH hardness and DOC)

bull To make calculating aluminum criteria easier EPA developed an Aluminum Criteria Calculatorndash Automated so that user only needs to enter water chemistry

conditions of interestndash Allows quick calculations for a variety of conditionsndash Limits calculations to the recommended conditions for which

the MLR models were developed were specified for hardness and DOC

bull No extrapolation beyond hardness and DOC empirical data bounds

23

Aluminum Criteria Calculator SD Example

24

pH=7 Hardness=100 DOC=1 pH=6 Hardness=25 DOC=1

Chronic Ranked GMCVRank GMCV Genus

12 17098 Aeolosoma Invert11 gt 9746 Rana Amphib - Other Data10 5908 Chironomus Invert

9 4877 Lymnaea Mollusk8 2555 Brachionus Invert7 2488 Pimephales Fish6 1713 Hyalella Invert5 1182 Ceriodaphnia Invert4 1102 Danio Fish3 1042 Lampsilis Mollusk2 591 Salvelinus Fish1 509 Salmo Fish

FCV = 3940 microgL total aluminum

Chronic Ranked GMCVRank GMCV Genus

12 gt 2131 Rana Amphib - Other Data11 1710 Aeolosoma Invert10 591 Chironomus Invert

9 544 Pimephales Fish8 488 Lymnaea Mollusk7 255 Brachionus Invert6 241 Danio Fish5 171 Hyalella Invert4 129 Salvelinus Fish3 118 Ceriodaphnia Invert2 111 Salmo Fish1 104 Lampsilis Mollusk

FCV = 987 microgL total aluminum

Multiple Scenarios

Run Scenarios

Summary Sheet

Acute Dataset

Chronic Dataset

Multiple Scenarios

Run Scenarios

Summary Sheet

Acute Dataset

Chronic Dataset

Boundaries of the Criteria Outputsbull MLR models were developed using data that

encompassndash DOC range of 008-5 mgLndash Hardness range of 98-127 mgL (as CaCO3)ndash pH range of 6-81

bull MLR criteria outputs are bounded atndash Maximum of 150 mgL total hardness (as CaCO3)

ndash DOC of 50 mgLndash pH range of the model is 50 to 90

25

Aluminum Criteria Look-Up Tables

26

bull EPA also provided criteria look-up tables in Appendix K

Har

dnes

s

CCC(microgL total aluminum)

(DOC=05 mgL)

pH 50 pH 55 pH 60 pH 65 pH 70 pH 75 pH 80 pH 85 pH 90

25 23 a 14 b 69 c 160 f 350 f 620 i 540 b 410 a 230 a

50 43 a 24 b 100 d 170 f 310 f 630 f 670 j 400 a 200 a

75 61 a 33 b 130 e 180 f 290 f 530 f 730 i 390 a 190 a

100 78 a 41 b 150 e 190 f 280 g 460 f 770 c 390 a 180 a

150 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

200 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

250 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

300 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

350 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

400 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

Aluminum Criteria Look-Up Tables

27

bull EPA also provided criteria look-up tables in Appendix K

Har

dnes

s

CCC(microgL total aluminum)

(DOC=10 mgL)

pH 50 pH 55 pH 60 pH 65 pH 70 pH 75 pH 80 pH 85 pH 90

25 33 a 21 b 99 c 220 f 490 f 900 i 780 b 590 a 330 a

50 61 a 35 b 150 e 250 f 440 f 890 f 960 j 580 a 290 a

75 88 a 47 b 180 e 260 f 410 f 740 f 1100 i 570 a 270 a

100 11 a 59 b 210 e 270 f 390 g 650 f 1100 c 560 b 260 a

150 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

200 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

250 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

300 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

350 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

400 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

Aluminum Criteria Look-Up Tables

28

bull EPA also provided criteria look-up tables in Appendix K

Har

dnes

s

CCC(microgL total aluminum)

(DOC=25 mgL)

pH 50 pH 55 pH 60 pH 65 pH 70 pH 75 pH 80 pH 85 pH 90

25 54 a 34 b 160 c 350 f 770 f 1500 i 1300 b 960 a 540 a

50 99 a 57 b 230 e 390 f 690 f 1400 f 1600 j 930 a 470 a

75 14 a 77 b 290 e 410 f 640 f 1200 f 1700 i 920 a 440 a

100 18 a 96 b 330 e 420 f 620 g 1000 f 1800 d 920 b 420 a

150 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

200 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

250 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

300 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

350 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

400 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

Aluminum Criteria Look-Up Tables

29

bull EPA also provided criteria look-up tables in Appendix K

Har

dnes

s

CCC(microgL total aluminum)

(DOC=50 mgL)

pH 50 pH 55 pH 60 pH 65 pH 70 pH 75 pH 80 pH 85 pH 90

25 77 a 49 b 230 c 500 f 1100 f 2100 c 1900 b 1400 a 770 a

50 14 a 82 b 330 e 540 f 970 f 2000 f 2200 j 1300 a 680 a

75 20 a 110 b 410 e 570 f 900 f 1600 f 2500 i 1300 a 640 a

100 26 a 140 b 460 f 590 f 880 g 1400 f 2500 d 1300 b 600 a

150 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

200 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

250 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

300 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

350 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

400 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

Status

30

bull Draft Document Released to the Federal Register on 72817ndash Public comment period of 60 days ndash Due Date for Comments is 92617

bull Revisions will be made considering public commentsbull Subsequent EPA intra-agency review of final criteria

document will then be conducted bull Publication of final criteria document planned for Late Fall

2017

Federal Register Webpagehttpswwwfederalregistergovdocuments201

707282017-15968request-for-scientific-views-draft-updated-aquatic-life-ambient-water-quality-criteria-for-aluminum

Aluminum Criteria Webpagehttpswwwepagovwqc2017-draft-aquatic-life-

criteria-aluminum-freshwater-documents

31

	Appendix C Acceptable Chronic Toxicity Data of Aluminum to Freshwater Aquatic Animals
									MLR equation
	Species		Hardness (mgL as CaCO3)		pH		DOC(mgL)		EC20 Endpoint			EC20(microgL)			Normalized Conc- EC20(microgL)			SMCV-EC20(microgL)		GMCV-EC20(microgL)			Genus			DOC Notes		Dilution water
	Oligochaete Aeolosoma sp		48		6		025		Reproduction (population size)			1235			1710			1710		1710			Aeolosoma		Invert		Author reported half the detection limit
	Rotifer Brachionus calyciflorus		100		62		025		Reproduction (population size)			431			255			255		255			Brachionus		Invert		Author reported half the detection limit
	Great pond snail Lymnaea stagnalis		117		61		025		Biomass			746			488			488		488			Lymnaea		Mollusk		Author reported half the detection limit
	Fatmucket Lampsilis siliquoidea		1055		604		040		Biomass			169			104			104		104			Lampsilis		Mollusk		Author reported
	Cladoceran Ceriodaphnia dubia		50		715		11		Reproduction - youngstarting adult			1780			190						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		26		73		05		Reproduction - youngfemale			1557			272						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		46		75		05		Reproduction - youngfemale			8087			110						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		96		79		05		Reproduction - youngfemale			6519			80						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		194		81		05		Reproduction - youngfemale			6836			89						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		255		703		05		Reproduction (youngfemale)			250			56						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		122		713		05		Reproduction (youngfemale)			860			106						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		25		798		05		Reproduction (youngfemale)			700			101						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		61		803		05		Reproduction (youngfemale)			1010			136						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		122		810		05		Reproduction (youngfemale)			870			116						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		255		633		05		Reproduction (youngfemale)			260			176						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		121		636		05		Reproduction (youngfemale)			390			110			118		118			Ceriodaphnia		Invert		Author reported
	Amphipod Hyalella azteca		95		61		051		Biomass (28 d)			1993			101						Hyalella			Author reported
	Amphipod Hyalella azteca		106		604		033		Biomass (28 d)			425			289			171		171			Hyalella		Invert		Author reported
	Midge Chironomus riparius		118		558		18		Adult midge emergence			2955			119						Chironomus			Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Midge Chironomus riparius		119		505		18		Adult midge emergence			8442			2258						Chironomus			Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Midge Chironomus riparius		91		66		051		Reproduction ( of eggscase)			3387			766			591		591			Chironomus		Invert		Author reported
	Atlantic salmon Salmo salar		127		57		18		Biomass			6156			1112			1112		1112			Salmo		Fish		Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Brook trout Salvelinus fontinalis		123		655		19		Biomass			1644			59						Salvelinus			Author reported		wellRO mix
	Brook trout Salvelinus fontinalis		128		565		18		Biomass			1435			283			1293		1293			Salvelinus		Fish		Author reported		wellRO mix
	Fathead minnow Pimephales promelas		220		770		16		Biomass			6194			781						Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		well water
	Fathead minnow Pimephales promelas		96		620		025		Fry survival			4286			379			544		544			Pimephales		Fish		Author reported half the detection limit
	Zebrafish Danio rerio		83		61		025		Biomass			2344			241			241		241			Danio		Fish		Author reported half the detection limit
	Wood frog Rana sylvatica		115		469		16		MATC-survival		gt		2000		gt		2131		gt		2131		2131		gt		Rana		Amphib - Other Data		Value is from 2007 FW Cu AWQC Appendix C recommendation		aged tap water

	Appendix A Acceptable Acute Toxicity Data of Aluminum to Freshwater Aquatic Animals
	Species		Methoda		Hardness (mgL as CaCO3)		pH		DOC(mgL)			LC50 or EC50 Conc (microgL)		Reference			Normalized Conc - LC50(microgL)			Species Mean Acute Value(microgL)		Genus Mean Acute Value(microgL)			Genus			DOC Note		Dilution water
	Snail Physa sp		S M T		474		659		11		gt		23400		Call 1984 Call et al 1984		gt		4843			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		755		11			30600		Call 1984 Call et al 1984			2699			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		817		11		gt		24700		Call 1984 Call et al 1984		gt		2404			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		746		11			55500		Call 1984 Call et al 1984			5034			3546		3546			Physa		Mollusk		Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Fatmucket Lampsilis siliquoidea		F M T		1060		612		048		gt		6302		Wang et al 2016 Wang et al 2017		gt		2983		gt		2983		2983		gt		Lampsilis		Mollusk		Author reported
	Cladoceran Ceriodaphnia dubia		S M A		500		742		11			1900		McCauley et al 1986			173			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		S M A		505		786		11			1500		McCauley et al 1986			129			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		UW-S Lake Superior lab water
	Cladoceran Ceriodaphnia dubia		S M A		500		813		11			2560		McCauley et al 1986			243			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		R M T		26		75		05			720		ENSR 1992d			111			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		R M T		46		76		05			1880		ENSR 1992d			250			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		R M T		96		78		05			2450		ENSR 1992d			289			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		S U NR		90		715		05			3727		Fort and Stover 1995			500			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U NR		90		715		05			5673		Fort and Stover 1995			761			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U NR		89		76		05			2880		Soucek et al 2001			336			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		601		05			7112		European Al Association 2009			169			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		605		2			6865		European Al Association 2009			709			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		609		4			15581		European Al Association 2009			1007			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		601		05			681		European Al Association 2009			162			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		603		05			1630		European Al Association 2009			367			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		597		05			1785		European Al Association 2009			473			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		592		05			1410		European Al Association 2009			429			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		699		05		gt		1300		European Al Association 2009		gt		419			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		785		05		gt		5000		European Al Association 2009		gt		796			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		68		2		gt		10000		European Al Association 2009		gt		2076			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		782		2		gt		15000		European Al Association 2009		gt		1163			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		677		4		gt		10000		European Al Association 2009		gt		1516			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		766		4		gt		15000		European Al Association 2009		gt		861			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		79		05		gt		2000		European Al Association 2009		gt		315			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		789		05		gt		2000		European Al Association 2009		gt		316			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		604		05			1108		European Al Association 2009			85			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		598		2			11371		European Al Association 2009			482			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		573		4			80467		European Al Association 2009			4357			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		671		05		gt		10000		European Al Association 2009		gt		2379			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		783		05		gt		5000		European Al Association 2009		gt		634			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		679		2		gt		10000		European Al Association 2009		gt		1039			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		767		2		gt		15000		European Al Association 2009		gt		908			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		668		4		gt		15000		European Al Association 2009		gt		1246			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		762		4		gt		15000		European Al Association 2009		gt		633			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		606		2			33868		European Al Association 2009			792			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		56		4			104842		European Al Association 2009			4880			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		693		05		gt		5000		European Al Association 2009		gt		713			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		120		788		05		gt		5000		European Al Association 2009		gt		590			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		120		676		2		gt		15000		European Al Association 2009		gt		1212			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		771		2		gt		15000		European Al Association 2009		gt		810			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		66		4		gt		15000		European Al Association 2009		gt		1014			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		76		4		gt		15000		European Al Association 2009		gt		555			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			11971		European Al Association 2010			270			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			27478		European Al Association 2010			619			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			11998		European Al Association 2010			270			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		607		05			92495		European Al Association 2010			188			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		609		05			31337		European Al Association 2010			604			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		61		05			33235		European Al Association 2010			624			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		708		05		gt		8864		European Al Association 2010		gt		253			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		779		05		gt		42783		European Al Association 2010		gt		693			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		753		05			13204		European Al Association 2010			24			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		600		601		05			46326		European Al Association 2010			380			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		600		599		05		gt		8590		European Al Association 2010		gt		737			509				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia reticulata		F M T		451		60		11			304		Shephard 1983			201			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia reticulata		F M T		40		55		11			362		Shephard 1983			5531			1054		733			Ceriodaphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake SuperiorDI Mix
	Cladoceran Daphnia magna		S U NR		453		774		11			3900		Biesinger and Christensen 1972			338			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Daphnia magna		S M T		220		76		16			38200		Kimball 1978			2030			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Well water
	Cladoceran Daphnia magna		S U T		451		725		11			2800		Shephard 1983			287			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Daphnia magna		S U T		168		599		05			5000		European Al Association 2009		gt		227			-				Daphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Daphnia magna		S U T		168		792		05			7950		European Al Association 2009			92			333		333			Daphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Ostracod Stenocypris major		R M T		1563		651		16			310196		Shuhaimi-Othman et al 2011a			1022			1022		1022			Stenocypris		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (Malaysia)
	Amphipod Crangonyx pseudogracilis		R U T		50		675		16			9190		Martin and Holdich 1986			1217			1217		1217			Crangonyx		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (UK)
	Amphipod Hyalella azteca		F M T		105		613		048		gt		5997		Wang et al 2016 Wang et al 2017		gt		2800		gt		2800		2800		gt		Hyalella		Invert		Author reported
	Stonefly Acroneuria sp		S M T		474		746		11		gt		22600		Call et al 1984		gt		2050		gt		2050		2050		gt		Acroneuria		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Midge Chironomus plumosus		S U T		80		70		16			30000		Fargasova 2001			2536			2536		2536			Chironomus		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (Slovakia)
	Midge Paratanytarsus dissimilis		S M T		1743		728		28		gt		77700		Lamb and Bailey 1981 1983		gt		6232		gt		6232		6232		gt		Paratanytarsus		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Liberty Lake Washington
	Rainbow trout Oncorhynchus mykiss		F M T		274		758		05		gt		9840		Gundersen et al 1994		gt		1256			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		452		762		05		gt		8070		Gundersen et al 1994		gt		1140			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		895		758		05		gt		8160		Gundersen et al 1994		gt		1489			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		1304		758		05		gt		8200		Gundersen et al 1994		gt		1676			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		232		825		05			6170		Gundersen et al 1994			260			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		350		826		05			6170		Gundersen et al 1994			333			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		836		829		05			7670		Gundersen et al 1994			701			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		1158		830		05			6930		Gundersen et al 1994			777			801		801			Oncorhynchus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Atlantic salmon Salmo salar		S U T		68		55		05			584		Hamilton and Haines 1995			4602			-				Salmo			Value is from 2007 FW Cu AWQC Appendix C recommendation		Soft recon
	Atlantic salmon Salmo salar		S U T		68		65		05			599		Hamilton and Haines 1995			531			1564		1564			Salmo		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Soft recon
	Brook trout Salvelinus fontinalis		S U T		40		56		16			6530		Tandjung 1982			6947			-				Salvelinus			Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Brook trout Salvelinus fontinalis		S U T		18		56		16			3400		Tandjung 1982			6209			-				Salvelinus			Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Brook trout Salvelinus fontinalis		S U T		2		56		16			370		Tandjung 1982			2988			5051		5051			Salvelinus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Green sunfish Lepomis cyanellus		S M T		474		755		11		gt		50000		Call et al 1984		gt		5255		gt		5255		5255		gt		Lepomis		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Rio Grande silvery minnow Hybognathus amarus		R M T		140		81		05		gt		59100		Buhl 2002		gt		8620		gt		8620		8620		gt		Hybognathus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon water
	Fathead minnow Pimephales promelas		S M T		474		761		11		gt		48200		Call et al 1984		gt		4707			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Fathead minnow Pimephales promelas		S M T		474		805		11		gt		49800		Call et al 1984		gt		2837			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Fathead minnow Pimephales promelas		R M T		26		78		05			1160		ENSR 1992c			104			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		46		76		05			8180		ENSR 1992c			1191			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		96		81		05			20300		ENSR 1992c			2398			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		194		81		05			44800		ENSR 1992c			7839			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		140		81		05		gt		59100		Buhl 2002		gt		8620			2224		2224			Pimephales		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon water
	Smallmouth bass Micropterus dolomieui		S M T		1245		505		16			130		Kane and Rabeni 1987			859			859		859			Micropterus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Green tree frog Hyla cinerea		R M T		455		549		05		gt		4052		Jung and Jagoe 1995		gt		4377		gt		4377		4377		gt		Hyla		Amphibian		Value is from 2007 FW Cu AWQC Appendix C recommendation		artificial soft recon

	Normalization Chemistry
	pH		6					FAV		360
	Hardness		25					CMC		180
	DOC		1					CCC		99
	Acute Ranked GMAV											Chronic Ranked GMCV
	Rank			GMAV		Genus								Rank			GMCV		Genus
	18		gt		8620		Hybognathus		Fish							12		gt		2131		Rana		Amphib - Other Data
	17		gt		6232		Paratanytarsus		Invert							11			1710		Aeolosoma		Invert
	16		gt		5255		Lepomis		Fish							10			591		Chironomus		Invert
	15			5051		Salvelinus		Fish							9			544		Pimephales		Fish
	14		gt		4377		Hyla		Amphibian							8			488		Lymnaea		Mollusk
	13			3546		Physa		Mollusk							7			255		Brachionus		Invert
	12		gt		2983		Lampsilis		Mollusk							6			241		Danio		Fish
	11		gt		2800		Hyalella		Invert							5			171		Hyalella		Invert
	10			2536		Chironomus		Invert							4			129		Salvelinus		Fish
	9			2224		Pimephales		Fish							3			118		Ceriodaphnia		Invert
	8		gt		2050		Acroneuria		Invert							2			111		Salmo		Fish
	7			1564		Salmo		Fish							1			104		Lampsilis		Mollusk
	6			1217		Crangonyx		Invert
	5			1022		Stenocypris		Invert
	4			859		Micropterus		Fish
	3			801		Oncorhynchus		Fish
	2			733		Ceriodaphnia		Invert
	1			333		Daphnia		Invert
		N		Rank		Genus		GMAV		ln(GMAV)		ln(GMAV)2		P=R(N+1)		sqrt(P)				N		Rank		Genus		GMCV		ln(GMCV)		ln(GMCV)2		P=R(N+1)		sqrt(P)
		18		4		Micropterus		859		676		4564		0211		0459				12		4		Salvelinus		129		486		2364		0308		0555
			3		Oncorhynchus		801		669		4469		0158		0397					3		Ceriodaphnia		118		477		2277		0231		0480
			2		Ceriodaphnia		733		660		4352		0105		0324					2		Salmo		111		471		2220		0154		0392
			1		Daphnia		333		581		3374		0053		0229					1		Lampsilis		104		465		2159		0077		0277
			Sum				2585		1676		0526		141					Sum				1899		902		0769		170
			S2 =		1987										S2 =		059
			L =		4890										L =		4420
			A =		5887										A =		4592
			FAV =		360										FCV =		987
			CMC =		180

				All concentrations reported are microgL total Aluminum
				lt-----Click Button after entering data
	ENTER DATA HERE									ERRORNUM		ACUTE														CHRONIC
										Rank 1				Rank 2				Rank 3				Rank 4					Rank 1				Rank 2				Rank 3				Rank 4
	SiteName		DOC(mgL)		Hardness(mgL as CaCO3)		pH			FAV		CMC		CCC			Genus		GMAV			Genus		GMAV			Genus		GMAV			Genus		GMAV				Genus		GMCV			Genus		GMCV			Genus		GMCV			Genus		GMCV
	Site 1		50		25		9		75428737034162		35229324666008		1800		770			Daphnia			33452		Ceriodaphnia			73576		Stenocypris			102576		Crangonyx			122230			Lampsilis			10463		Ceriodaphnia			11865		Hyalella			17200		Brachionus			25648
	Site 2		50		50		9		75428737034162		31204991509063		1600		680			Daphnia			29631		Ceriodaphnia			65171		Stenocypris			90859		Crangonyx			108267			Lampsilis			9268		Ceriodaphnia			10510		Hyalella			15236		Brachionus			22718
	Site 3		50		75		9		75428737034162		29067527928199		1500		640			Daphnia			27601		Ceriodaphnia			60707		Stenocypris			84635		Crangonyx			100851			Lampsilis			8633		Ceriodaphnia			9790		Hyalella			14192		Brachionus			21162
	Site 4		50		100		9		75428737034162		27640367912592		1400		600			Daphnia			26246		Ceriodaphnia			57726		Stenocypris			80480		Crangonyx			95899			Lampsilis			8209		Ceriodaphnia			9309		Hyalella			13495		Brachionus			20123
	Site 5		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 6		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 7		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 8		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 9		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 10		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 11		50		25		85		75428737034162		63245275664384		3200		1400			Daphnia			60055		Ceriodaphnia			132086		Stenocypris			184149		Crangonyx			219432			Lampsilis			18783		Ceriodaphnia			21301		Hyalella			30879		Brachionus			46045
	Site 12		50		50		85		75428737034162		61388044609926		3100		1300			Daphnia			58291		Ceriodaphnia			128208		Stenocypris			178742		Crangonyx			212988			Lampsilis			18232		Ceriodaphnia			20676		Hyalella			29972		Brachionus			44693
	Site 13		50		75		85		75428737034162		60327020429132		3000		1300			Daphnia			57284		Ceriodaphnia			125992		Stenocypris			175652		Crangonyx			209307			Lampsilis			17917		Ceriodaphnia			20318		Hyalella			29454		Brachionus			43920
	Site 14		50		100		85		75428737034162		59585352130146		3000		1300			Daphnia			56580		Ceriodaphnia			124443		Stenocypris			173493		Crangonyx			206734			Lampsilis			17696		Ceriodaphnia			20068		Hyalella			29092		Salmo			41264
	Site 15		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 16		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 17		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 18		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 19		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 20		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370

	Appendix C Acceptable Chronic Toxicity Data of Aluminum to Freshwater Aquatic Animals
									MLR equation
	Species		Hardness (mgL as CaCO3)		pH		DOC(mgL)		EC20 Endpoint			EC20(microgL)			Normalized Conc- EC20(microgL)			SMCV-EC20(microgL)		GMCV-EC20(microgL)			Genus			DOC Notes		Dilution water
	Oligochaete Aeolosoma sp		48		6		025		Reproduction (population size)			1235			17098			17098		17098			Aeolosoma		Invert		Author reported half the detection limit
	Rotifer Brachionus calyciflorus		100		62		025		Reproduction (population size)			431			2555			2555		2555			Brachionus		Invert		Author reported half the detection limit
	Great pond snail Lymnaea stagnalis		117		61		025		Biomass			746			4877			4877		4877			Lymnaea		Mollusk		Author reported half the detection limit
	Fatmucket Lampsilis siliquoidea		1055		604		040		Biomass			169			1042			1042		1042			Lampsilis		Mollusk		Author reported
	Cladoceran Ceriodaphnia dubia		50		715		11		Reproduction - youngstarting adult			1780			1898						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		26		73		05		Reproduction - youngfemale			1557			2718						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		46		75		05		Reproduction - youngfemale			8087			1102						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		96		79		05		Reproduction - youngfemale			6519			796						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		194		81		05		Reproduction - youngfemale			6836			888						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		255		703		05		Reproduction (youngfemale)			250			563						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		122		713		05		Reproduction (youngfemale)			860			1061						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		25		798		05		Reproduction (youngfemale)			700			1006						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		61		803		05		Reproduction (youngfemale)			1010			1363						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		122		810		05		Reproduction (youngfemale)			870			1164						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		255		633		05		Reproduction (youngfemale)			260			1758						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		121		636		05		Reproduction (youngfemale)			390			1101			1182		1182			Ceriodaphnia		Invert		Author reported
	Amphipod Hyalella azteca		95		61		051		Biomass (28 d)			1993			1014						Hyalella			Author reported
	Amphipod Hyalella azteca		106		604		033		Biomass (28 d)			425			2895			1713		1713			Hyalella		Invert		Author reported
	Midge Chironomus riparius		118		558		18		Adult midge emergence			2955			1192						Chironomus			Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Midge Chironomus riparius		119		505		18		Adult midge emergence			8442			22578						Chironomus			Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Midge Chironomus riparius		91		66		051		Reproduction ( of eggscase)			3387			7664			5908		5908			Chironomus		Invert		Author reported
	Atlantic salmon Salmo salar		127		57		18		Biomass			6156			5085			5085		5085			Salmo		Fish		Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Brook trout Salvelinus fontinalis		123		655		19		Biomass			1644			270						Salvelinus			Author reported		wellRO mix
	Brook trout Salvelinus fontinalis		128		565		18		Biomass			1435			1295			5914		5914			Salvelinus		Fish		Author reported		wellRO mix
	Fathead minnow Pimephales promelas		220		770		16		Biomass			6194			3569						Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		well water
	Fathead minnow Pimephales promelas		96		620		025		Fry survival			4286			1734			2488		2488			Pimephales		Fish		Author reported half the detection limit
	Zebrafish Danio rerio		83		61		025		Biomass			2344			1102			1102		1102			Danio		Fish		Author reported half the detection limit
	Wood frog Rana sylvatica		115		469		16		MATC-survival		gt		2000		gt		9746		gt		9746		9746		gt		Rana		Amphib - Other Data		Value is from 2007 FW Cu AWQC Appendix C recommendation		aged tap water

	Appendix A Acceptable Acute Toxicity Data of Aluminum to Freshwater Aquatic Animals
	Species		Methoda		Hardness (mgL as CaCO3)		pH		DOC(mgL)			LC50 or EC50 Conc (microgL)		Reference			Normalized Conc - LC50(microgL)			Species Mean Acute Value(microgL)		Genus Mean Acute Value(microgL)			Genus			DOC Note		Dilution water
	Snail Physa sp		S M T		474		659		11		gt		23400		Call 1984 Call et al 1984		gt		48425			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		755		11			30600		Call 1984 Call et al 1984			26986			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		817		11		gt		24700		Call 1984 Call et al 1984		gt		24040			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		746		11			55500		Call 1984 Call et al 1984			50338			35462		35462			Physa		Mollusk		Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Fatmucket Lampsilis siliquoidea		F M T		1060		612		048		gt		6302		Wang et al 2016 Wang et al 2017		gt		29834		gt		29834		29834		gt		Lampsilis		Mollusk		Author reported
	Cladoceran Ceriodaphnia dubia		S M A		500		742		11			1900		McCauley et al 1986			1728			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		S M A		505		786		11			1500		McCauley et al 1986			1294			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		UW-S Lake Superior lab water
	Cladoceran Ceriodaphnia dubia		S M A		500		813		11			2560		McCauley et al 1986			2431			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		R M T		26		75		05			720		ENSR 1992d			1113			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		R M T		46		76		05			1880		ENSR 1992d			2495			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		R M T		96		78		05			2450		ENSR 1992d			2894			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		S U NR		90		715		05			3727		Fort and Stover 1995			5002			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U NR		90		715		05			5673		Fort and Stover 1995			7613			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U NR		89		76		05			2880		Soucek et al 2001			3362			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		601		05			7112		European Al Association 2009			1691			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		605		2			6865		European Al Association 2009			7088			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		609		4			15581		European Al Association 2009			10072			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		601		05			681		European Al Association 2009			1620			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		603		05			1630		European Al Association 2009			3674			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		597		05			1785		European Al Association 2009			4732			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		592		05			1410		European Al Association 2009			4295			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		699		05		gt		1300		European Al Association 2009		gt		4189			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		785		05		gt		5000		European Al Association 2009		gt		7960			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		68		2		gt		10000		European Al Association 2009		gt		20755			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		782		2		gt		15000		European Al Association 2009		gt		11626			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		677		4		gt		10000		European Al Association 2009		gt		15161			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		766		4		gt		15000		European Al Association 2009		gt		8608			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		79		05		gt		2000		European Al Association 2009		gt		3150			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		789		05		gt		2000		European Al Association 2009		gt		3156			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		604		05			1108		European Al Association 2009			850			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		598		2			11371		European Al Association 2009			4817			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		573		4			80467		European Al Association 2009			43570			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		671		05		gt		10000		European Al Association 2009		gt		23786			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		783		05		gt		5000		European Al Association 2009		gt		6344			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		679		2		gt		10000		European Al Association 2009		gt		10394			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		767		2		gt		15000		European Al Association 2009		gt		9082			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		668		4		gt		15000		European Al Association 2009		gt		12461			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		762		4		gt		15000		European Al Association 2009		gt		6332			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		606		2			33868		European Al Association 2009			7920			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		56		4			104842		European Al Association 2009			48800			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		693		05		gt		5000		European Al Association 2009		gt		7126			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		120		788		05		gt		5000		European Al Association 2009		gt		5898			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		120		676		2		gt		15000		European Al Association 2009		gt		12117			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		771		2		gt		15000		European Al Association 2009		gt		8096			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		66		4		gt		15000		European Al Association 2009		gt		10139			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		76		4		gt		15000		European Al Association 2009		gt		5545			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			11971		European Al Association 2010			2699			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			27478		European Al Association 2010			6194			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			11998		European Al Association 2010			2705			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		607		05			92495		European Al Association 2010			1876			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		609		05			31337		European Al Association 2010			6035			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		61		05			33235		European Al Association 2010			6238			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		708		05		gt		8864		European Al Association 2010		gt		2534			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		779		05		gt		42783		European Al Association 2010		gt		6930			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		753		05			13204		European Al Association 2010			244			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		600		601		05			46326		European Al Association 2010			3798			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		600		599		05		gt		8590		European Al Association 2010		gt		7366			5094				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia reticulata		F M T		451		60		11			304		Shephard 1983			2009			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia reticulata		F M T		40		55		11			362		Shephard 1983			55307			10542		7328			Ceriodaphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake SuperiorDI Mix
	Cladoceran Daphnia magna		S U NR		453		774		11			3900		Biesinger and Christensen 1972			3382			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Daphnia magna		S M T		220		76		16			38200		Kimball 1978			20303			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Well water
	Cladoceran Daphnia magna		S U T		451		725		11			2800		Shephard 1983			2868			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Daphnia magna		S U T		168		599		05			5000		European Al Association 2009		gt		2265			-				Daphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Daphnia magna		S U T		168		792		05			7950		European Al Association 2009			920			3332		3332			Daphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Ostracod Stenocypris major		R M T		1563		651		16			310196		Shuhaimi-Othman et al 2011a			10216			10216		10216			Stenocypris		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (Malaysia)
	Amphipod Crangonyx pseudogracilis		R U T		50		675		16			9190		Martin and Holdich 1986			12174			12174		12174			Crangonyx		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (UK)
	Amphipod Hyalella azteca		F M T		105		613		048		gt		5997		Wang et al 2016 Wang et al 2017		gt		28002		gt		28002		28002		gt		Hyalella		Invert		Author reported
	Stonefly Acroneuria sp		S M T		474		746		11		gt		22600		Call et al 1984		gt		20498		gt		20498		20498		gt		Acroneuria		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Midge Chironomus plumosus		S U T		80		70		16			30000		Fargasova 2001			25361			25361		25361			Chironomus		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (Slovakia)
	Midge Paratanytarsus dissimilis		S M T		1743		728		28		gt		77700		Lamb and Bailey 1981 1983		gt		62318		gt		62318		62318		gt		Paratanytarsus		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Liberty Lake Washington
	Rainbow trout Oncorhynchus mykiss		F M T		274		758		05		gt		9840		Gundersen et al 1994		gt		5745			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		452		762		05		gt		8070		Gundersen et al 1994		gt		5212			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		895		758		05		gt		8160		Gundersen et al 1994		gt		6807			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		1304		758		05		gt		8200		Gundersen et al 1994		gt		7662			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		232		825		05			6170		Gundersen et al 1994			1190			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		350		826		05			6170		Gundersen et al 1994			1523			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		836		829		05			7670		Gundersen et al 1994			3205			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		1158		830		05			6930		Gundersen et al 1994			3554			3661		3661			Oncorhynchus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Atlantic salmon Salmo salar		S U T		68		55		05			584		Hamilton and Haines 1995			21042			-				Salmo			Value is from 2007 FW Cu AWQC Appendix C recommendation		Soft recon
	Atlantic salmon Salmo salar		S U T		68		65		05			599		Hamilton and Haines 1995			2430			7151		7151			Salmo		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Soft recon
	Brook trout Salvelinus fontinalis		S U T		40		56		16			6530		Tandjung 1982			31767			-				Salvelinus			Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Brook trout Salvelinus fontinalis		S U T		18		56		16			3400		Tandjung 1982			28391			-				Salvelinus			Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Brook trout Salvelinus fontinalis		S U T		2		56		16			370		Tandjung 1982			13663			23097		23097			Salvelinus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Green sunfish Lepomis cyanellus		S M T		474		755		11		gt		50000		Call et al 1984		gt		24028		gt		24028		24028		gt		Lepomis		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Rio Grande silvery minnow Hybognathus amarus		R M T		140		81		05		gt		59100		Buhl 2002		gt		39414		gt		39414		39414		gt		Hybognathus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon water
	Fathead minnow Pimephales promelas		S M T		474		761		11		gt		48200		Call et al 1984		gt		21522			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Fathead minnow Pimephales promelas		S M T		474		805		11		gt		49800		Call et al 1984		gt		12972			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Fathead minnow Pimephales promelas		R M T		26		78		05			1160		ENSR 1992c			477			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		46		76		05			8180		ENSR 1992c			5446			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		96		81		05			20300		ENSR 1992c			10966			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		194		81		05			44800		ENSR 1992c			35847			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		140		81		05		gt		59100		Buhl 2002		gt		39414			10168		10168			Pimephales		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon water
	Smallmouth bass Micropterus dolomieui		S M T		1245		505		16			130		Kane and Rabeni 1987			3929			3929		3929			Micropterus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Green tree frog Hyla cinerea		R M T		455		549		05		gt		4052		Jung and Jagoe 1995		gt		20016		gt		20016		20016		gt		Hyla		Amphibian		Value is from 2007 FW Cu AWQC Appendix C recommendation		artificial soft recon

	Normalization Chemistry
	pH		7					FAV		2741
	Hardness		100					CMC		1400
	DOC		1					CCC		390
	Acute Ranked GMAV											Chronic Ranked GMCV
	Rank			GMAV		Genus								Rank			GMCV		Genus
	18		gt		62318		Paratanytarsus		Invert							12			17098		Aeolosoma		Invert
	17		gt		39414		Hybognathus		Fish							11		gt		9746		Rana		Amphib - Other Data
	16			35462		Physa		Mollusk							10			5908		Chironomus		Invert
	15		gt		29834		Lampsilis		Mollusk							9			4877		Lymnaea		Mollusk
	14		gt		28002		Hyalella		Invert							8			2555		Brachionus		Invert
	13			25361		Chironomus		Invert							7			2488		Pimephales		Fish
	12		gt		24028		Lepomis		Fish							6			1713		Hyalella		Invert
	11			23097		Salvelinus		Fish							5			1182		Ceriodaphnia		Invert
	10		gt		20498		Acroneuria		Invert							4			1102		Danio		Fish
	9		gt		20016		Hyla		Amphibian							3			1042		Lampsilis		Mollusk
	8			12174		Crangonyx		Invert							2			591		Salvelinus		Fish
	7			10216		Stenocypris		Invert							1			509		Salmo		Fish
	6			10168		Pimephales		Fish
	5			7328		Ceriodaphnia		Invert
	4			7151		Salmo		Fish
	3			3929		Micropterus		Fish
	2			3661		Oncorhynchus		Fish
	1			3332		Daphnia		Invert
		N		Rank		Genus		GMAV		ln(GMAV)		ln(GMAV)2		P=R(N+1)		sqrt(P)				N		Rank		Genus		GMCV		ln(GMCV)		ln(GMCV)2		P=R(N+1)		sqrt(P)
		18		4		Salmo		7151		887		7876		0211		0459				12		4		Danio		1102		700		4907		0308		0555
			3		Micropterus		3929		828		6849		0158		0397					3		Lampsilis		1042		695		4829		0231		0480
			2		Oncorhynchus		3661		821		6733		0105		0324					2		Salvelinus		591		638		4074		0154		0392
			1		Daphnia		3332		811		6579		0053		0229					1		Salmo		509		623		3883		0077		0277
			Sum				3347		2804		0526		141					Sum				2657		1769		0769		170
			S2 =		1223										S2 =		1080
			L =		7134										L =		5242
			A =		7916										A =		5976
			FAV =		2741										FCV =		3940
			CMC =		1371

				All concentrations reported are microgL total Aluminum
				lt-----Click Button after entering data
	ENTER DATA HERE									ERRORNUM		ACUTE														CHRONIC
										Rank 1				Rank 2				Rank 3				Rank 4					Rank 1				Rank 2				Rank 3				Rank 4
	SiteName		DOC(mgL)		Hardness(mgL as CaCO3)		pH			FAV		CMC		CCC			Genus		GMAV			Genus		GMAV			Genus		GMAV			Genus		GMAV				Genus		GMCV			Genus		GMCV			Genus		GMCV			Genus		GMCV
	Site 1		50		25		9		75428737034162		35229324666008		1800		770			Daphnia			33452		Ceriodaphnia			73576		Stenocypris			102576		Crangonyx			122230			Lampsilis			10463		Ceriodaphnia			11865		Hyalella			17200		Brachionus			25648
	Site 2		50		50		9		75428737034162		31204991509063		1600		680			Daphnia			29631		Ceriodaphnia			65171		Stenocypris			90859		Crangonyx			108267			Lampsilis			9268		Ceriodaphnia			10510		Hyalella			15236		Brachionus			22718
	Site 3		50		75		9		75428737034162		29067527928199		1500		640			Daphnia			27601		Ceriodaphnia			60707		Stenocypris			84635		Crangonyx			100851			Lampsilis			8633		Ceriodaphnia			9790		Hyalella			14192		Brachionus			21162
	Site 4		50		100		9		75428737034162		27640367912592		1400		600			Daphnia			26246		Ceriodaphnia			57726		Stenocypris			80480		Crangonyx			95899			Lampsilis			8209		Ceriodaphnia			9309		Hyalella			13495		Brachionus			20123
	Site 5		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 6		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 7		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 8		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 9		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 10		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 11		50		25		85		75428737034162		63245275664384		3200		1400			Daphnia			60055		Ceriodaphnia			132086		Stenocypris			184149		Crangonyx			219432			Lampsilis			18783		Ceriodaphnia			21301		Hyalella			30879		Brachionus			46045
	Site 12		50		50		85		75428737034162		61388044609926		3100		1300			Daphnia			58291		Ceriodaphnia			128208		Stenocypris			178742		Crangonyx			212988			Lampsilis			18232		Ceriodaphnia			20676		Hyalella			29972		Brachionus			44693
	Site 13		50		75		85		75428737034162		60327020429132		3000		1300			Daphnia			57284		Ceriodaphnia			125992		Stenocypris			175652		Crangonyx			209307			Lampsilis			17917		Ceriodaphnia			20318		Hyalella			29454		Brachionus			43920
	Site 14		50		100		85		75428737034162		59585352130146		3000		1300			Daphnia			56580		Ceriodaphnia			124443		Stenocypris			173493		Crangonyx			206734			Lampsilis			17696		Ceriodaphnia			20068		Hyalella			29092		Salmo			41264
	Site 15		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 16		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 17		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 18		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 19		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 20		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370

bull Attendees who are members of the media can submit any questions to the press office Trisha Lynn (202) 564-2615

2

Overview

Background 2017 Recommended Criteria Status

3

Sources of Aluminumbull Aluminum is found in most rocks and

minerals and can enter the environment through natural weathering

bull Aluminumrsquos solubility increase under acidic (pH lt6) or basic (pH gt8) water conditions

bull Aluminum is actively mined from bauxite used in industrial processes and alum (potassium aluminum sulfate) is used in the clarification process at drinking water facilities 4

Current 1988 Aluminum Criteriabull The current 1988 criteria account for the

ndash Influence of pH on toxicity (solely by limiting criteria application range)

ndash Similarity of acute toxicity of fish and invertebrates

ndash The greater sensitivity of invertebrates in chronic toxicity tests

5

Current 1988 Aluminum Criteriabull The final 1988 criteria document recommended

ndash An acute criterion of 750 microgL total aluminum when the pH is between 65 and 90

ndash Chronic criterion of 87 microgL total aluminum when the pH is between 65 and 90

ndash The 1988 freshwater chronic criterion of 87 microgL was lowered based on two tests (brook trout and striped bass)

ndash The studies were not pH adjusted and all the data were not normalized for hardness The 1988 existing criteria did not take hardness and DOC into account

6

Aluminum Standardsbull Aluminum Standards

ndash About 20 states have numeric criteria for aluminum for aquatic life

bull Most are based on EPArsquos 1988 values and two are hardness-based equations (CO and NM)

bull There are numerous site-specific criteria that involve the Biotic Ligand Model (BLM) or a Water Effects Ratio (WER) -adjusted 1988 aluminum criteria

ndash Aluminum is not a priority pollutant

7

Criteria Development Processbull All EPA Aquatic Life Criteria for Toxics undergo rigorous

scientific development and review processbull Develop criteria document draftbull Intra-agency peer review process bull Independent external peer reviewbull Revisions based on external peer review

Intra-agency review of draft criteria documentbull Release to the public in draft form to obtain scientific views

Revisions considering public comments Intra-agency review of final criteria document

bull Publication of final criteria document bull States may then consider recommended criteria in

development and adoption of state water quality standards8

Framework for Updated Draft Aluminum Criteria

I Screen toxicological studies for high

quality data

II Consider water conditions effects on

aluminum bioavailability

III Normalize toxicity data in species

sensitivity distribution to a set of water quality conditions

IV Construct sensitivity distribution to determine criteria

at set conditions

9

I Screen toxicological studies for high quality data

bull First step in all criteria developmentndash Data are evaluated based on the data quality objectives

outlined in the 1985 Guidelines methodology and other toxicity testing recommendations (ie EPA ASTM)

ndash High quality data are used quantitatively to develop criteria magnitudes

ndash Lower quality data are used qualitatively if appropriatendash Lowest quality data are considered unacceptable and not

usedbull Data are grouped into acute and chronic exposures

10

Aluminum Toxicity Databull The updated freshwater aluminum criteria meets the

acute and chronic minimum data requirements outlined in EPArsquos 1985 Guidelines

InvertebrateSpecies

Fish Species

AmphibianSpecies

Total Genera

Acute1988 AWQC 8 7 - 142017 Draft AWQC 11 8 1 18

Chronic1988 AWQC 2 1 - 32017 Draft AWQC 7 4 - 11

11 1988 AWQC Chronic CCC based on two studies for the striped

bass and brook trout

Aluminum Toxicity Data Freshwater mussels have been

suggested to be potentially sensitive to aluminum

EPA worked collaboratively with USGSrsquos Columbia Environmental Research Center (CERC) to have acute and chronic aluminum toxicity tests conducted with a unionid mussel (fatmucket Lampsilis siliquoidea) in water-only exposures to obtain additional data on invertebrates New data on amphipod (Hyalella azteca)

were collected to confirm laboratory conditions

12

Aluminum Toxicity Data

13

bull New data and models examinedndash Data submitted to EU REACH program

bull New freshwater aluminum toxicity data generated by Oregon State University

bull 2016 BLM model provided by the European Aluminum Association

ndash Saltwater data provided by Australia bull Insufficient data to develop estuarinemarine acute or chronic

criteria at this timendash Multiple Linear Regression (MLR) model developed by

DeForest et al 2017bull DeForest DK KV Brix LM Tear and WJ Adams 2017 Multiple Linear Regression (MLR) models for predicting chronic aluminum

toxicity to freshwater aquatic organisms and developing water quality guidelines Environ Toxicol Chem (DOI101002etc3922)

14

Minimum Dataset For Freshwater Criteria Derivation

SALMONID SECONDFISHFAMILY

CHORDATA

PLANKTONICCRUSTACEAN

BENTHIC CRUSTACEAN

INSECT ROTIFERA ANNELIDA MOLLUSCA

OTHERINSECT ORMOLLUSCA

EstuarineMarine Minimum Data Requirements

15

Family Minimum Data Requirement EstuarineMarine

Acute(Phylum Family Genus)

Chronic(Phylum Family Genus)

Family in the phylum Chordata - -

Family in the phylum Chordata - -

Either the Mysidae or Penaeidae family - -

Family in a phylum other than Arthropoda or Chordata Mollusca Ostreidae Crassostrea -

Family in a phylum other than Chordata Annelida Nereididae Neanthes -

Family in a phylum other than Chordata Annelida Capitellidae Capitella -

Family in a phylum other than Chordata Annelida Ctenodrilidae Ctenodrilus -

Any other family Arthropoda Ameiridae Nitokra -

II Evaluate water quality conditions to determine applicability

bull Criteria are often based on one or more water quality conditions (eg hardness) that affect toxicity of the material to test organisms

bull Aluminum toxicity is highly dependent on the pH of the test waterndash The pH of test waters affects the amount and form

of aluminum that is bioavailable to test speciesndash Hardness and DOC (Dissolved Organic Carbon)

also influence the amount of aluminum that is bioavailable

16

Multiple Bioavailability Modeling Approaches Underwent External Peer Review

17

bull Biotic Ligand Modelsbull Full aluminum BLM (2016 model version) ndash Temperature pH

Dissolved organic carbon (DOC) Major cations (calcium [Ca] magnesium [Mg] sodium [Na] and potassium [K]) Major anions (sulfate [SO4] and chloride [Cl]) Alkalinity and Sulfide

bull Simplified aluminum BLM - pH hardness DOC temperature

bull Multilinear Regression Equationsbull pH and Hardness

Accounting for Effects of Water Chemistry on Bioavailability and Toxicity

bull Water quality criteria can be modified quantitatively when enough data are available to demonstrate that those conditions similarly affect the results with a variety of speciesndash High quality data in the draft aluminum document demonstrated

that pH DOC and hardness affected a variety of species in a similar way when exposed to aluminum concentrations

bull Recent publications by Cardwell et al (2017) and Gensemer et al (2017) summarized short-term aluminum chronic toxicity data across a range of DOC pH and hardness values for three species

18

Multiple Linear Regression (MLR) Models to Account for Effects of Water Chemistry

bull MLR models were developed for each species (DeForest et al 2017)ndash 119862119862 119889119889119906119906119887119887119894119894119886119886 11986411986411986211986220=119890119890[minus41026+[0525timesln(119863119863119874119874119862119862)]+[2201timesln(ℎ119886119886119903119903119889119889)]+(11282times119901119901119867119867)minus(0633times1199011199011198671198672)minus[0264times119901119901119867119867ln(ℎ119886119886119903119903119889119889)]]

ndash 119875119875 119901119901119903119903119900119900119898119898119890119890119897119897119886119886119904119904 11986411986411986211986220=119890119890[minus14029+[0503timesln(119863119863119874119874119862119862)]+[3443timesln(ℎ119886119886119903119903119889119889)]+(3131times119901119901119867119867)minus[0494times119901119901119867119867ln(ℎ119886119886119903119903119889119889)]]

bull These models were applied to both the acute and chronic aluminum toxicity datandash The C dubia model was applied to invertebrate datandash The P promelas model was applied to vertebrate data

19

III Normalize toxicity data in species sensitivity distribution to a set of water

quality conditionsbull Just like single parameter hardness models the MLR

models were used to normalize the aluminum toxicity data to a single set of water quality conditionsndash As an illustration for one set of calculations the draft

aluminum AWQC set these conditions to pH 7 hardness of 100 mgL and DOC of 1 mgL

bull This allows for comparisons to be made between species based on their sensitivity to aluminumndash Apples to apples

20

IV Construct sensitivity distribution to determine criteria at set conditions

21

Figure 8 Ranked Summary of Total Aluminum Genus Mean Acute Values (GMAVs) - Freshwater at pH 7 Hardness of 100 mgL and DOC of 1 mgL

IV Construct sensitivity distribution to determine criteria at set conditions

22

Figure 10 Ranked Summary of Total Aluminum Genus Mean Chronic Values (GMCVs) ndash Freshwater Supplemented with Other Data to Fulfill Missing MDRs at pH 7 Hardness of 100 mgL and DOC of 1 mgL

Aluminum Criteria Calculatorbull The relative ranking of genera in the sensitivity

distribution to which the toxicity data are normalized will change based on the set of water chemistry conditions (ie pH hardness and DOC)

bull To make calculating aluminum criteria easier EPA developed an Aluminum Criteria Calculatorndash Automated so that user only needs to enter water chemistry

conditions of interestndash Allows quick calculations for a variety of conditionsndash Limits calculations to the recommended conditions for which

the MLR models were developed were specified for hardness and DOC

bull No extrapolation beyond hardness and DOC empirical data bounds

23

Aluminum Criteria Calculator SD Example

24

pH=7 Hardness=100 DOC=1 pH=6 Hardness=25 DOC=1

Chronic Ranked GMCVRank GMCV Genus

12 17098 Aeolosoma Invert11 gt 9746 Rana Amphib - Other Data10 5908 Chironomus Invert

9 4877 Lymnaea Mollusk8 2555 Brachionus Invert7 2488 Pimephales Fish6 1713 Hyalella Invert5 1182 Ceriodaphnia Invert4 1102 Danio Fish3 1042 Lampsilis Mollusk2 591 Salvelinus Fish1 509 Salmo Fish

FCV = 3940 microgL total aluminum

Chronic Ranked GMCVRank GMCV Genus

12 gt 2131 Rana Amphib - Other Data11 1710 Aeolosoma Invert10 591 Chironomus Invert

9 544 Pimephales Fish8 488 Lymnaea Mollusk7 255 Brachionus Invert6 241 Danio Fish5 171 Hyalella Invert4 129 Salvelinus Fish3 118 Ceriodaphnia Invert2 111 Salmo Fish1 104 Lampsilis Mollusk

FCV = 987 microgL total aluminum

Multiple Scenarios

Run Scenarios

Summary Sheet

Acute Dataset

Chronic Dataset

Multiple Scenarios

Run Scenarios

Summary Sheet

Acute Dataset

Chronic Dataset

Boundaries of the Criteria Outputsbull MLR models were developed using data that

encompassndash DOC range of 008-5 mgLndash Hardness range of 98-127 mgL (as CaCO3)ndash pH range of 6-81

bull MLR criteria outputs are bounded atndash Maximum of 150 mgL total hardness (as CaCO3)

ndash DOC of 50 mgLndash pH range of the model is 50 to 90

25

Aluminum Criteria Look-Up Tables

26

bull EPA also provided criteria look-up tables in Appendix K

Har

dnes

s

CCC(microgL total aluminum)

(DOC=05 mgL)

pH 50 pH 55 pH 60 pH 65 pH 70 pH 75 pH 80 pH 85 pH 90

25 23 a 14 b 69 c 160 f 350 f 620 i 540 b 410 a 230 a

50 43 a 24 b 100 d 170 f 310 f 630 f 670 j 400 a 200 a

75 61 a 33 b 130 e 180 f 290 f 530 f 730 i 390 a 190 a

100 78 a 41 b 150 e 190 f 280 g 460 f 770 c 390 a 180 a

150 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

200 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

250 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

300 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

350 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

400 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

Aluminum Criteria Look-Up Tables

27

bull EPA also provided criteria look-up tables in Appendix K

Har

dnes

s

CCC(microgL total aluminum)

(DOC=10 mgL)

pH 50 pH 55 pH 60 pH 65 pH 70 pH 75 pH 80 pH 85 pH 90

25 33 a 21 b 99 c 220 f 490 f 900 i 780 b 590 a 330 a

50 61 a 35 b 150 e 250 f 440 f 890 f 960 j 580 a 290 a

75 88 a 47 b 180 e 260 f 410 f 740 f 1100 i 570 a 270 a

100 11 a 59 b 210 e 270 f 390 g 650 f 1100 c 560 b 260 a

150 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

200 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

250 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

300 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

350 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

400 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

Aluminum Criteria Look-Up Tables

28

bull EPA also provided criteria look-up tables in Appendix K

Har

dnes

s

CCC(microgL total aluminum)

(DOC=25 mgL)

pH 50 pH 55 pH 60 pH 65 pH 70 pH 75 pH 80 pH 85 pH 90

25 54 a 34 b 160 c 350 f 770 f 1500 i 1300 b 960 a 540 a

50 99 a 57 b 230 e 390 f 690 f 1400 f 1600 j 930 a 470 a

75 14 a 77 b 290 e 410 f 640 f 1200 f 1700 i 920 a 440 a

100 18 a 96 b 330 e 420 f 620 g 1000 f 1800 d 920 b 420 a

150 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

200 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

250 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

300 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

350 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

400 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

Aluminum Criteria Look-Up Tables

29

bull EPA also provided criteria look-up tables in Appendix K

Har

dnes

s

CCC(microgL total aluminum)

(DOC=50 mgL)

pH 50 pH 55 pH 60 pH 65 pH 70 pH 75 pH 80 pH 85 pH 90

25 77 a 49 b 230 c 500 f 1100 f 2100 c 1900 b 1400 a 770 a

50 14 a 82 b 330 e 540 f 970 f 2000 f 2200 j 1300 a 680 a

75 20 a 110 b 410 e 570 f 900 f 1600 f 2500 i 1300 a 640 a

100 26 a 140 b 460 f 590 f 880 g 1400 f 2500 d 1300 b 600 a

150 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

200 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

250 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

300 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

350 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

400 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

Status

30

bull Draft Document Released to the Federal Register on 72817ndash Public comment period of 60 days ndash Due Date for Comments is 92617

bull Revisions will be made considering public commentsbull Subsequent EPA intra-agency review of final criteria

document will then be conducted bull Publication of final criteria document planned for Late Fall

2017

Federal Register Webpagehttpswwwfederalregistergovdocuments201

707282017-15968request-for-scientific-views-draft-updated-aquatic-life-ambient-water-quality-criteria-for-aluminum

Aluminum Criteria Webpagehttpswwwepagovwqc2017-draft-aquatic-life-

criteria-aluminum-freshwater-documents

31

	Appendix C Acceptable Chronic Toxicity Data of Aluminum to Freshwater Aquatic Animals
									MLR equation
	Species		Hardness (mgL as CaCO3)		pH		DOC(mgL)		EC20 Endpoint			EC20(microgL)			Normalized Conc- EC20(microgL)			SMCV-EC20(microgL)		GMCV-EC20(microgL)			Genus			DOC Notes		Dilution water
	Oligochaete Aeolosoma sp		48		6		025		Reproduction (population size)			1235			1710			1710		1710			Aeolosoma		Invert		Author reported half the detection limit
	Rotifer Brachionus calyciflorus		100		62		025		Reproduction (population size)			431			255			255		255			Brachionus		Invert		Author reported half the detection limit
	Great pond snail Lymnaea stagnalis		117		61		025		Biomass			746			488			488		488			Lymnaea		Mollusk		Author reported half the detection limit
	Fatmucket Lampsilis siliquoidea		1055		604		040		Biomass			169			104			104		104			Lampsilis		Mollusk		Author reported
	Cladoceran Ceriodaphnia dubia		50		715		11		Reproduction - youngstarting adult			1780			190						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		26		73		05		Reproduction - youngfemale			1557			272						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		46		75		05		Reproduction - youngfemale			8087			110						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		96		79		05		Reproduction - youngfemale			6519			80						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		194		81		05		Reproduction - youngfemale			6836			89						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		255		703		05		Reproduction (youngfemale)			250			56						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		122		713		05		Reproduction (youngfemale)			860			106						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		25		798		05		Reproduction (youngfemale)			700			101						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		61		803		05		Reproduction (youngfemale)			1010			136						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		122		810		05		Reproduction (youngfemale)			870			116						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		255		633		05		Reproduction (youngfemale)			260			176						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		121		636		05		Reproduction (youngfemale)			390			110			118		118			Ceriodaphnia		Invert		Author reported
	Amphipod Hyalella azteca		95		61		051		Biomass (28 d)			1993			101						Hyalella			Author reported
	Amphipod Hyalella azteca		106		604		033		Biomass (28 d)			425			289			171		171			Hyalella		Invert		Author reported
	Midge Chironomus riparius		118		558		18		Adult midge emergence			2955			119						Chironomus			Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Midge Chironomus riparius		119		505		18		Adult midge emergence			8442			2258						Chironomus			Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Midge Chironomus riparius		91		66		051		Reproduction ( of eggscase)			3387			766			591		591			Chironomus		Invert		Author reported
	Atlantic salmon Salmo salar		127		57		18		Biomass			6156			1112			1112		1112			Salmo		Fish		Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Brook trout Salvelinus fontinalis		123		655		19		Biomass			1644			59						Salvelinus			Author reported		wellRO mix
	Brook trout Salvelinus fontinalis		128		565		18		Biomass			1435			283			1293		1293			Salvelinus		Fish		Author reported		wellRO mix
	Fathead minnow Pimephales promelas		220		770		16		Biomass			6194			781						Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		well water
	Fathead minnow Pimephales promelas		96		620		025		Fry survival			4286			379			544		544			Pimephales		Fish		Author reported half the detection limit
	Zebrafish Danio rerio		83		61		025		Biomass			2344			241			241		241			Danio		Fish		Author reported half the detection limit
	Wood frog Rana sylvatica		115		469		16		MATC-survival		gt		2000		gt		2131		gt		2131		2131		gt		Rana		Amphib - Other Data		Value is from 2007 FW Cu AWQC Appendix C recommendation		aged tap water

	Appendix A Acceptable Acute Toxicity Data of Aluminum to Freshwater Aquatic Animals
	Species		Methoda		Hardness (mgL as CaCO3)		pH		DOC(mgL)			LC50 or EC50 Conc (microgL)		Reference			Normalized Conc - LC50(microgL)			Species Mean Acute Value(microgL)		Genus Mean Acute Value(microgL)			Genus			DOC Note		Dilution water
	Snail Physa sp		S M T		474		659		11		gt		23400		Call 1984 Call et al 1984		gt		4843			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		755		11			30600		Call 1984 Call et al 1984			2699			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		817		11		gt		24700		Call 1984 Call et al 1984		gt		2404			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		746		11			55500		Call 1984 Call et al 1984			5034			3546		3546			Physa		Mollusk		Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Fatmucket Lampsilis siliquoidea		F M T		1060		612		048		gt		6302		Wang et al 2016 Wang et al 2017		gt		2983		gt		2983		2983		gt		Lampsilis		Mollusk		Author reported
	Cladoceran Ceriodaphnia dubia		S M A		500		742		11			1900		McCauley et al 1986			173			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		S M A		505		786		11			1500		McCauley et al 1986			129			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		UW-S Lake Superior lab water
	Cladoceran Ceriodaphnia dubia		S M A		500		813		11			2560		McCauley et al 1986			243			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		R M T		26		75		05			720		ENSR 1992d			111			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		R M T		46		76		05			1880		ENSR 1992d			250			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		R M T		96		78		05			2450		ENSR 1992d			289			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		S U NR		90		715		05			3727		Fort and Stover 1995			500			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U NR		90		715		05			5673		Fort and Stover 1995			761			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U NR		89		76		05			2880		Soucek et al 2001			336			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		601		05			7112		European Al Association 2009			169			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		605		2			6865		European Al Association 2009			709			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		609		4			15581		European Al Association 2009			1007			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		601		05			681		European Al Association 2009			162			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		603		05			1630		European Al Association 2009			367			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		597		05			1785		European Al Association 2009			473			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		592		05			1410		European Al Association 2009			429			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		699		05		gt		1300		European Al Association 2009		gt		419			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		785		05		gt		5000		European Al Association 2009		gt		796			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		68		2		gt		10000		European Al Association 2009		gt		2076			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		782		2		gt		15000		European Al Association 2009		gt		1163			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		677		4		gt		10000		European Al Association 2009		gt		1516			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		766		4		gt		15000		European Al Association 2009		gt		861			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		79		05		gt		2000		European Al Association 2009		gt		315			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		789		05		gt		2000		European Al Association 2009		gt		316			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		604		05			1108		European Al Association 2009			85			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		598		2			11371		European Al Association 2009			482			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		573		4			80467		European Al Association 2009			4357			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		671		05		gt		10000		European Al Association 2009		gt		2379			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		783		05		gt		5000		European Al Association 2009		gt		634			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		679		2		gt		10000		European Al Association 2009		gt		1039			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		767		2		gt		15000		European Al Association 2009		gt		908			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		668		4		gt		15000		European Al Association 2009		gt		1246			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		762		4		gt		15000		European Al Association 2009		gt		633			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		606		2			33868		European Al Association 2009			792			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		56		4			104842		European Al Association 2009			4880			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		693		05		gt		5000		European Al Association 2009		gt		713			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		120		788		05		gt		5000		European Al Association 2009		gt		590			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		120		676		2		gt		15000		European Al Association 2009		gt		1212			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		771		2		gt		15000		European Al Association 2009		gt		810			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		66		4		gt		15000		European Al Association 2009		gt		1014			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		76		4		gt		15000		European Al Association 2009		gt		555			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			11971		European Al Association 2010			270			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			27478		European Al Association 2010			619			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			11998		European Al Association 2010			270			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		607		05			92495		European Al Association 2010			188			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		609		05			31337		European Al Association 2010			604			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		61		05			33235		European Al Association 2010			624			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		708		05		gt		8864		European Al Association 2010		gt		253			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		779		05		gt		42783		European Al Association 2010		gt		693			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		753		05			13204		European Al Association 2010			24			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		600		601		05			46326		European Al Association 2010			380			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		600		599		05		gt		8590		European Al Association 2010		gt		737			509				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia reticulata		F M T		451		60		11			304		Shephard 1983			201			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia reticulata		F M T		40		55		11			362		Shephard 1983			5531			1054		733			Ceriodaphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake SuperiorDI Mix
	Cladoceran Daphnia magna		S U NR		453		774		11			3900		Biesinger and Christensen 1972			338			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Daphnia magna		S M T		220		76		16			38200		Kimball 1978			2030			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Well water
	Cladoceran Daphnia magna		S U T		451		725		11			2800		Shephard 1983			287			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Daphnia magna		S U T		168		599		05			5000		European Al Association 2009		gt		227			-				Daphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Daphnia magna		S U T		168		792		05			7950		European Al Association 2009			92			333		333			Daphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Ostracod Stenocypris major		R M T		1563		651		16			310196		Shuhaimi-Othman et al 2011a			1022			1022		1022			Stenocypris		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (Malaysia)
	Amphipod Crangonyx pseudogracilis		R U T		50		675		16			9190		Martin and Holdich 1986			1217			1217		1217			Crangonyx		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (UK)
	Amphipod Hyalella azteca		F M T		105		613		048		gt		5997		Wang et al 2016 Wang et al 2017		gt		2800		gt		2800		2800		gt		Hyalella		Invert		Author reported
	Stonefly Acroneuria sp		S M T		474		746		11		gt		22600		Call et al 1984		gt		2050		gt		2050		2050		gt		Acroneuria		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Midge Chironomus plumosus		S U T		80		70		16			30000		Fargasova 2001			2536			2536		2536			Chironomus		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (Slovakia)
	Midge Paratanytarsus dissimilis		S M T		1743		728		28		gt		77700		Lamb and Bailey 1981 1983		gt		6232		gt		6232		6232		gt		Paratanytarsus		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Liberty Lake Washington
	Rainbow trout Oncorhynchus mykiss		F M T		274		758		05		gt		9840		Gundersen et al 1994		gt		1256			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		452		762		05		gt		8070		Gundersen et al 1994		gt		1140			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		895		758		05		gt		8160		Gundersen et al 1994		gt		1489			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		1304		758		05		gt		8200		Gundersen et al 1994		gt		1676			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		232		825		05			6170		Gundersen et al 1994			260			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		350		826		05			6170		Gundersen et al 1994			333			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		836		829		05			7670		Gundersen et al 1994			701			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		1158		830		05			6930		Gundersen et al 1994			777			801		801			Oncorhynchus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Atlantic salmon Salmo salar		S U T		68		55		05			584		Hamilton and Haines 1995			4602			-				Salmo			Value is from 2007 FW Cu AWQC Appendix C recommendation		Soft recon
	Atlantic salmon Salmo salar		S U T		68		65		05			599		Hamilton and Haines 1995			531			1564		1564			Salmo		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Soft recon
	Brook trout Salvelinus fontinalis		S U T		40		56		16			6530		Tandjung 1982			6947			-				Salvelinus			Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Brook trout Salvelinus fontinalis		S U T		18		56		16			3400		Tandjung 1982			6209			-				Salvelinus			Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Brook trout Salvelinus fontinalis		S U T		2		56		16			370		Tandjung 1982			2988			5051		5051			Salvelinus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Green sunfish Lepomis cyanellus		S M T		474		755		11		gt		50000		Call et al 1984		gt		5255		gt		5255		5255		gt		Lepomis		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Rio Grande silvery minnow Hybognathus amarus		R M T		140		81		05		gt		59100		Buhl 2002		gt		8620		gt		8620		8620		gt		Hybognathus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon water
	Fathead minnow Pimephales promelas		S M T		474		761		11		gt		48200		Call et al 1984		gt		4707			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Fathead minnow Pimephales promelas		S M T		474		805		11		gt		49800		Call et al 1984		gt		2837			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Fathead minnow Pimephales promelas		R M T		26		78		05			1160		ENSR 1992c			104			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		46		76		05			8180		ENSR 1992c			1191			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		96		81		05			20300		ENSR 1992c			2398			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		194		81		05			44800		ENSR 1992c			7839			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		140		81		05		gt		59100		Buhl 2002		gt		8620			2224		2224			Pimephales		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon water
	Smallmouth bass Micropterus dolomieui		S M T		1245		505		16			130		Kane and Rabeni 1987			859			859		859			Micropterus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Green tree frog Hyla cinerea		R M T		455		549		05		gt		4052		Jung and Jagoe 1995		gt		4377		gt		4377		4377		gt		Hyla		Amphibian		Value is from 2007 FW Cu AWQC Appendix C recommendation		artificial soft recon

	Normalization Chemistry
	pH		6					FAV		360
	Hardness		25					CMC		180
	DOC		1					CCC		99
	Acute Ranked GMAV											Chronic Ranked GMCV
	Rank			GMAV		Genus								Rank			GMCV		Genus
	18		gt		8620		Hybognathus		Fish							12		gt		2131		Rana		Amphib - Other Data
	17		gt		6232		Paratanytarsus		Invert							11			1710		Aeolosoma		Invert
	16		gt		5255		Lepomis		Fish							10			591		Chironomus		Invert
	15			5051		Salvelinus		Fish							9			544		Pimephales		Fish
	14		gt		4377		Hyla		Amphibian							8			488		Lymnaea		Mollusk
	13			3546		Physa		Mollusk							7			255		Brachionus		Invert
	12		gt		2983		Lampsilis		Mollusk							6			241		Danio		Fish
	11		gt		2800		Hyalella		Invert							5			171		Hyalella		Invert
	10			2536		Chironomus		Invert							4			129		Salvelinus		Fish
	9			2224		Pimephales		Fish							3			118		Ceriodaphnia		Invert
	8		gt		2050		Acroneuria		Invert							2			111		Salmo		Fish
	7			1564		Salmo		Fish							1			104		Lampsilis		Mollusk
	6			1217		Crangonyx		Invert
	5			1022		Stenocypris		Invert
	4			859		Micropterus		Fish
	3			801		Oncorhynchus		Fish
	2			733		Ceriodaphnia		Invert
	1			333		Daphnia		Invert
		N		Rank		Genus		GMAV		ln(GMAV)		ln(GMAV)2		P=R(N+1)		sqrt(P)				N		Rank		Genus		GMCV		ln(GMCV)		ln(GMCV)2		P=R(N+1)		sqrt(P)
		18		4		Micropterus		859		676		4564		0211		0459				12		4		Salvelinus		129		486		2364		0308		0555
			3		Oncorhynchus		801		669		4469		0158		0397					3		Ceriodaphnia		118		477		2277		0231		0480
			2		Ceriodaphnia		733		660		4352		0105		0324					2		Salmo		111		471		2220		0154		0392
			1		Daphnia		333		581		3374		0053		0229					1		Lampsilis		104		465		2159		0077		0277
			Sum				2585		1676		0526		141					Sum				1899		902		0769		170
			S2 =		1987										S2 =		059
			L =		4890										L =		4420
			A =		5887										A =		4592
			FAV =		360										FCV =		987
			CMC =		180

				All concentrations reported are microgL total Aluminum
				lt-----Click Button after entering data
	ENTER DATA HERE									ERRORNUM		ACUTE														CHRONIC
										Rank 1				Rank 2				Rank 3				Rank 4					Rank 1				Rank 2				Rank 3				Rank 4
	SiteName		DOC(mgL)		Hardness(mgL as CaCO3)		pH			FAV		CMC		CCC			Genus		GMAV			Genus		GMAV			Genus		GMAV			Genus		GMAV				Genus		GMCV			Genus		GMCV			Genus		GMCV			Genus		GMCV
	Site 1		50		25		9		75428737034162		35229324666008		1800		770			Daphnia			33452		Ceriodaphnia			73576		Stenocypris			102576		Crangonyx			122230			Lampsilis			10463		Ceriodaphnia			11865		Hyalella			17200		Brachionus			25648
	Site 2		50		50		9		75428737034162		31204991509063		1600		680			Daphnia			29631		Ceriodaphnia			65171		Stenocypris			90859		Crangonyx			108267			Lampsilis			9268		Ceriodaphnia			10510		Hyalella			15236		Brachionus			22718
	Site 3		50		75		9		75428737034162		29067527928199		1500		640			Daphnia			27601		Ceriodaphnia			60707		Stenocypris			84635		Crangonyx			100851			Lampsilis			8633		Ceriodaphnia			9790		Hyalella			14192		Brachionus			21162
	Site 4		50		100		9		75428737034162		27640367912592		1400		600			Daphnia			26246		Ceriodaphnia			57726		Stenocypris			80480		Crangonyx			95899			Lampsilis			8209		Ceriodaphnia			9309		Hyalella			13495		Brachionus			20123
	Site 5		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 6		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 7		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 8		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 9		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 10		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 11		50		25		85		75428737034162		63245275664384		3200		1400			Daphnia			60055		Ceriodaphnia			132086		Stenocypris			184149		Crangonyx			219432			Lampsilis			18783		Ceriodaphnia			21301		Hyalella			30879		Brachionus			46045
	Site 12		50		50		85		75428737034162		61388044609926		3100		1300			Daphnia			58291		Ceriodaphnia			128208		Stenocypris			178742		Crangonyx			212988			Lampsilis			18232		Ceriodaphnia			20676		Hyalella			29972		Brachionus			44693
	Site 13		50		75		85		75428737034162		60327020429132		3000		1300			Daphnia			57284		Ceriodaphnia			125992		Stenocypris			175652		Crangonyx			209307			Lampsilis			17917		Ceriodaphnia			20318		Hyalella			29454		Brachionus			43920
	Site 14		50		100		85		75428737034162		59585352130146		3000		1300			Daphnia			56580		Ceriodaphnia			124443		Stenocypris			173493		Crangonyx			206734			Lampsilis			17696		Ceriodaphnia			20068		Hyalella			29092		Salmo			41264
	Site 15		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 16		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 17		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 18		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 19		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 20		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370

	Appendix C Acceptable Chronic Toxicity Data of Aluminum to Freshwater Aquatic Animals
									MLR equation
	Species		Hardness (mgL as CaCO3)		pH		DOC(mgL)		EC20 Endpoint			EC20(microgL)			Normalized Conc- EC20(microgL)			SMCV-EC20(microgL)		GMCV-EC20(microgL)			Genus			DOC Notes		Dilution water
	Oligochaete Aeolosoma sp		48		6		025		Reproduction (population size)			1235			17098			17098		17098			Aeolosoma		Invert		Author reported half the detection limit
	Rotifer Brachionus calyciflorus		100		62		025		Reproduction (population size)			431			2555			2555		2555			Brachionus		Invert		Author reported half the detection limit
	Great pond snail Lymnaea stagnalis		117		61		025		Biomass			746			4877			4877		4877			Lymnaea		Mollusk		Author reported half the detection limit
	Fatmucket Lampsilis siliquoidea		1055		604		040		Biomass			169			1042			1042		1042			Lampsilis		Mollusk		Author reported
	Cladoceran Ceriodaphnia dubia		50		715		11		Reproduction - youngstarting adult			1780			1898						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		26		73		05		Reproduction - youngfemale			1557			2718						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		46		75		05		Reproduction - youngfemale			8087			1102						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		96		79		05		Reproduction - youngfemale			6519			796						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		194		81		05		Reproduction - youngfemale			6836			888						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		255		703		05		Reproduction (youngfemale)			250			563						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		122		713		05		Reproduction (youngfemale)			860			1061						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		25		798		05		Reproduction (youngfemale)			700			1006						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		61		803		05		Reproduction (youngfemale)			1010			1363						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		122		810		05		Reproduction (youngfemale)			870			1164						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		255		633		05		Reproduction (youngfemale)			260			1758						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		121		636		05		Reproduction (youngfemale)			390			1101			1182		1182			Ceriodaphnia		Invert		Author reported
	Amphipod Hyalella azteca		95		61		051		Biomass (28 d)			1993			1014						Hyalella			Author reported
	Amphipod Hyalella azteca		106		604		033		Biomass (28 d)			425			2895			1713		1713			Hyalella		Invert		Author reported
	Midge Chironomus riparius		118		558		18		Adult midge emergence			2955			1192						Chironomus			Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Midge Chironomus riparius		119		505		18		Adult midge emergence			8442			22578						Chironomus			Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Midge Chironomus riparius		91		66		051		Reproduction ( of eggscase)			3387			7664			5908		5908			Chironomus		Invert		Author reported
	Atlantic salmon Salmo salar		127		57		18		Biomass			6156			5085			5085		5085			Salmo		Fish		Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Brook trout Salvelinus fontinalis		123		655		19		Biomass			1644			270						Salvelinus			Author reported		wellRO mix
	Brook trout Salvelinus fontinalis		128		565		18		Biomass			1435			1295			5914		5914			Salvelinus		Fish		Author reported		wellRO mix
	Fathead minnow Pimephales promelas		220		770		16		Biomass			6194			3569						Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		well water
	Fathead minnow Pimephales promelas		96		620		025		Fry survival			4286			1734			2488		2488			Pimephales		Fish		Author reported half the detection limit
	Zebrafish Danio rerio		83		61		025		Biomass			2344			1102			1102		1102			Danio		Fish		Author reported half the detection limit
	Wood frog Rana sylvatica		115		469		16		MATC-survival		gt		2000		gt		9746		gt		9746		9746		gt		Rana		Amphib - Other Data		Value is from 2007 FW Cu AWQC Appendix C recommendation		aged tap water

	Appendix A Acceptable Acute Toxicity Data of Aluminum to Freshwater Aquatic Animals
	Species		Methoda		Hardness (mgL as CaCO3)		pH		DOC(mgL)			LC50 or EC50 Conc (microgL)		Reference			Normalized Conc - LC50(microgL)			Species Mean Acute Value(microgL)		Genus Mean Acute Value(microgL)			Genus			DOC Note		Dilution water
	Snail Physa sp		S M T		474		659		11		gt		23400		Call 1984 Call et al 1984		gt		48425			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		755		11			30600		Call 1984 Call et al 1984			26986			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		817		11		gt		24700		Call 1984 Call et al 1984		gt		24040			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		746		11			55500		Call 1984 Call et al 1984			50338			35462		35462			Physa		Mollusk		Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Fatmucket Lampsilis siliquoidea		F M T		1060		612		048		gt		6302		Wang et al 2016 Wang et al 2017		gt		29834		gt		29834		29834		gt		Lampsilis		Mollusk		Author reported
	Cladoceran Ceriodaphnia dubia		S M A		500		742		11			1900		McCauley et al 1986			1728			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		S M A		505		786		11			1500		McCauley et al 1986			1294			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		UW-S Lake Superior lab water
	Cladoceran Ceriodaphnia dubia		S M A		500		813		11			2560		McCauley et al 1986			2431			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		R M T		26		75		05			720		ENSR 1992d			1113			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		R M T		46		76		05			1880		ENSR 1992d			2495			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		R M T		96		78		05			2450		ENSR 1992d			2894			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		S U NR		90		715		05			3727		Fort and Stover 1995			5002			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U NR		90		715		05			5673		Fort and Stover 1995			7613			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U NR		89		76		05			2880		Soucek et al 2001			3362			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		601		05			7112		European Al Association 2009			1691			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		605		2			6865		European Al Association 2009			7088			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		609		4			15581		European Al Association 2009			10072			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		601		05			681		European Al Association 2009			1620			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		603		05			1630		European Al Association 2009			3674			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		597		05			1785		European Al Association 2009			4732			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		592		05			1410		European Al Association 2009			4295			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		699		05		gt		1300		European Al Association 2009		gt		4189			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		785		05		gt		5000		European Al Association 2009		gt		7960			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		68		2		gt		10000		European Al Association 2009		gt		20755			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		782		2		gt		15000		European Al Association 2009		gt		11626			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		677		4		gt		10000		European Al Association 2009		gt		15161			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		766		4		gt		15000		European Al Association 2009		gt		8608			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		79		05		gt		2000		European Al Association 2009		gt		3150			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		789		05		gt		2000		European Al Association 2009		gt		3156			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		604		05			1108		European Al Association 2009			850			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		598		2			11371		European Al Association 2009			4817			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		573		4			80467		European Al Association 2009			43570			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		671		05		gt		10000		European Al Association 2009		gt		23786			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		783		05		gt		5000		European Al Association 2009		gt		6344			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		679		2		gt		10000		European Al Association 2009		gt		10394			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		767		2		gt		15000		European Al Association 2009		gt		9082			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		668		4		gt		15000		European Al Association 2009		gt		12461			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		762		4		gt		15000		European Al Association 2009		gt		6332			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		606		2			33868		European Al Association 2009			7920			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		56		4			104842		European Al Association 2009			48800			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		693		05		gt		5000		European Al Association 2009		gt		7126			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		120		788		05		gt		5000		European Al Association 2009		gt		5898			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		120		676		2		gt		15000		European Al Association 2009		gt		12117			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		771		2		gt		15000		European Al Association 2009		gt		8096			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		66		4		gt		15000		European Al Association 2009		gt		10139			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		76		4		gt		15000		European Al Association 2009		gt		5545			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			11971		European Al Association 2010			2699			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			27478		European Al Association 2010			6194			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			11998		European Al Association 2010			2705			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		607		05			92495		European Al Association 2010			1876			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		609		05			31337		European Al Association 2010			6035			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		61		05			33235		European Al Association 2010			6238			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		708		05		gt		8864		European Al Association 2010		gt		2534			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		779		05		gt		42783		European Al Association 2010		gt		6930			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		753		05			13204		European Al Association 2010			244			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		600		601		05			46326		European Al Association 2010			3798			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		600		599		05		gt		8590		European Al Association 2010		gt		7366			5094				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia reticulata		F M T		451		60		11			304		Shephard 1983			2009			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia reticulata		F M T		40		55		11			362		Shephard 1983			55307			10542		7328			Ceriodaphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake SuperiorDI Mix
	Cladoceran Daphnia magna		S U NR		453		774		11			3900		Biesinger and Christensen 1972			3382			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Daphnia magna		S M T		220		76		16			38200		Kimball 1978			20303			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Well water
	Cladoceran Daphnia magna		S U T		451		725		11			2800		Shephard 1983			2868			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Daphnia magna		S U T		168		599		05			5000		European Al Association 2009		gt		2265			-				Daphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Daphnia magna		S U T		168		792		05			7950		European Al Association 2009			920			3332		3332			Daphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Ostracod Stenocypris major		R M T		1563		651		16			310196		Shuhaimi-Othman et al 2011a			10216			10216		10216			Stenocypris		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (Malaysia)
	Amphipod Crangonyx pseudogracilis		R U T		50		675		16			9190		Martin and Holdich 1986			12174			12174		12174			Crangonyx		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (UK)
	Amphipod Hyalella azteca		F M T		105		613		048		gt		5997		Wang et al 2016 Wang et al 2017		gt		28002		gt		28002		28002		gt		Hyalella		Invert		Author reported
	Stonefly Acroneuria sp		S M T		474		746		11		gt		22600		Call et al 1984		gt		20498		gt		20498		20498		gt		Acroneuria		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Midge Chironomus plumosus		S U T		80		70		16			30000		Fargasova 2001			25361			25361		25361			Chironomus		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (Slovakia)
	Midge Paratanytarsus dissimilis		S M T		1743		728		28		gt		77700		Lamb and Bailey 1981 1983		gt		62318		gt		62318		62318		gt		Paratanytarsus		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Liberty Lake Washington
	Rainbow trout Oncorhynchus mykiss		F M T		274		758		05		gt		9840		Gundersen et al 1994		gt		5745			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		452		762		05		gt		8070		Gundersen et al 1994		gt		5212			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		895		758		05		gt		8160		Gundersen et al 1994		gt		6807			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		1304		758		05		gt		8200		Gundersen et al 1994		gt		7662			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		232		825		05			6170		Gundersen et al 1994			1190			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		350		826		05			6170		Gundersen et al 1994			1523			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		836		829		05			7670		Gundersen et al 1994			3205			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		1158		830		05			6930		Gundersen et al 1994			3554			3661		3661			Oncorhynchus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Atlantic salmon Salmo salar		S U T		68		55		05			584		Hamilton and Haines 1995			21042			-				Salmo			Value is from 2007 FW Cu AWQC Appendix C recommendation		Soft recon
	Atlantic salmon Salmo salar		S U T		68		65		05			599		Hamilton and Haines 1995			2430			7151		7151			Salmo		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Soft recon
	Brook trout Salvelinus fontinalis		S U T		40		56		16			6530		Tandjung 1982			31767			-				Salvelinus			Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Brook trout Salvelinus fontinalis		S U T		18		56		16			3400		Tandjung 1982			28391			-				Salvelinus			Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Brook trout Salvelinus fontinalis		S U T		2		56		16			370		Tandjung 1982			13663			23097		23097			Salvelinus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Green sunfish Lepomis cyanellus		S M T		474		755		11		gt		50000		Call et al 1984		gt		24028		gt		24028		24028		gt		Lepomis		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Rio Grande silvery minnow Hybognathus amarus		R M T		140		81		05		gt		59100		Buhl 2002		gt		39414		gt		39414		39414		gt		Hybognathus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon water
	Fathead minnow Pimephales promelas		S M T		474		761		11		gt		48200		Call et al 1984		gt		21522			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Fathead minnow Pimephales promelas		S M T		474		805		11		gt		49800		Call et al 1984		gt		12972			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Fathead minnow Pimephales promelas		R M T		26		78		05			1160		ENSR 1992c			477			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		46		76		05			8180		ENSR 1992c			5446			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		96		81		05			20300		ENSR 1992c			10966			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		194		81		05			44800		ENSR 1992c			35847			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		140		81		05		gt		59100		Buhl 2002		gt		39414			10168		10168			Pimephales		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon water
	Smallmouth bass Micropterus dolomieui		S M T		1245		505		16			130		Kane and Rabeni 1987			3929			3929		3929			Micropterus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Green tree frog Hyla cinerea		R M T		455		549		05		gt		4052		Jung and Jagoe 1995		gt		20016		gt		20016		20016		gt		Hyla		Amphibian		Value is from 2007 FW Cu AWQC Appendix C recommendation		artificial soft recon

	Normalization Chemistry
	pH		7					FAV		2741
	Hardness		100					CMC		1400
	DOC		1					CCC		390
	Acute Ranked GMAV											Chronic Ranked GMCV
	Rank			GMAV		Genus								Rank			GMCV		Genus
	18		gt		62318		Paratanytarsus		Invert							12			17098		Aeolosoma		Invert
	17		gt		39414		Hybognathus		Fish							11		gt		9746		Rana		Amphib - Other Data
	16			35462		Physa		Mollusk							10			5908		Chironomus		Invert
	15		gt		29834		Lampsilis		Mollusk							9			4877		Lymnaea		Mollusk
	14		gt		28002		Hyalella		Invert							8			2555		Brachionus		Invert
	13			25361		Chironomus		Invert							7			2488		Pimephales		Fish
	12		gt		24028		Lepomis		Fish							6			1713		Hyalella		Invert
	11			23097		Salvelinus		Fish							5			1182		Ceriodaphnia		Invert
	10		gt		20498		Acroneuria		Invert							4			1102		Danio		Fish
	9		gt		20016		Hyla		Amphibian							3			1042		Lampsilis		Mollusk
	8			12174		Crangonyx		Invert							2			591		Salvelinus		Fish
	7			10216		Stenocypris		Invert							1			509		Salmo		Fish
	6			10168		Pimephales		Fish
	5			7328		Ceriodaphnia		Invert
	4			7151		Salmo		Fish
	3			3929		Micropterus		Fish
	2			3661		Oncorhynchus		Fish
	1			3332		Daphnia		Invert
		N		Rank		Genus		GMAV		ln(GMAV)		ln(GMAV)2		P=R(N+1)		sqrt(P)				N		Rank		Genus		GMCV		ln(GMCV)		ln(GMCV)2		P=R(N+1)		sqrt(P)
		18		4		Salmo		7151		887		7876		0211		0459				12		4		Danio		1102		700		4907		0308		0555
			3		Micropterus		3929		828		6849		0158		0397					3		Lampsilis		1042		695		4829		0231		0480
			2		Oncorhynchus		3661		821		6733		0105		0324					2		Salvelinus		591		638		4074		0154		0392
			1		Daphnia		3332		811		6579		0053		0229					1		Salmo		509		623		3883		0077		0277
			Sum				3347		2804		0526		141					Sum				2657		1769		0769		170
			S2 =		1223										S2 =		1080
			L =		7134										L =		5242
			A =		7916										A =		5976
			FAV =		2741										FCV =		3940
			CMC =		1371

				All concentrations reported are microgL total Aluminum
				lt-----Click Button after entering data
	ENTER DATA HERE									ERRORNUM		ACUTE														CHRONIC
										Rank 1				Rank 2				Rank 3				Rank 4					Rank 1				Rank 2				Rank 3				Rank 4
	SiteName		DOC(mgL)		Hardness(mgL as CaCO3)		pH			FAV		CMC		CCC			Genus		GMAV			Genus		GMAV			Genus		GMAV			Genus		GMAV				Genus		GMCV			Genus		GMCV			Genus		GMCV			Genus		GMCV
	Site 1		50		25		9		75428737034162		35229324666008		1800		770			Daphnia			33452		Ceriodaphnia			73576		Stenocypris			102576		Crangonyx			122230			Lampsilis			10463		Ceriodaphnia			11865		Hyalella			17200		Brachionus			25648
	Site 2		50		50		9		75428737034162		31204991509063		1600		680			Daphnia			29631		Ceriodaphnia			65171		Stenocypris			90859		Crangonyx			108267			Lampsilis			9268		Ceriodaphnia			10510		Hyalella			15236		Brachionus			22718
	Site 3		50		75		9		75428737034162		29067527928199		1500		640			Daphnia			27601		Ceriodaphnia			60707		Stenocypris			84635		Crangonyx			100851			Lampsilis			8633		Ceriodaphnia			9790		Hyalella			14192		Brachionus			21162
	Site 4		50		100		9		75428737034162		27640367912592		1400		600			Daphnia			26246		Ceriodaphnia			57726		Stenocypris			80480		Crangonyx			95899			Lampsilis			8209		Ceriodaphnia			9309		Hyalella			13495		Brachionus			20123
	Site 5		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 6		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 7		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 8		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 9		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 10		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 11		50		25		85		75428737034162		63245275664384		3200		1400			Daphnia			60055		Ceriodaphnia			132086		Stenocypris			184149		Crangonyx			219432			Lampsilis			18783		Ceriodaphnia			21301		Hyalella			30879		Brachionus			46045
	Site 12		50		50		85		75428737034162		61388044609926		3100		1300			Daphnia			58291		Ceriodaphnia			128208		Stenocypris			178742		Crangonyx			212988			Lampsilis			18232		Ceriodaphnia			20676		Hyalella			29972		Brachionus			44693
	Site 13		50		75		85		75428737034162		60327020429132		3000		1300			Daphnia			57284		Ceriodaphnia			125992		Stenocypris			175652		Crangonyx			209307			Lampsilis			17917		Ceriodaphnia			20318		Hyalella			29454		Brachionus			43920
	Site 14		50		100		85		75428737034162		59585352130146		3000		1300			Daphnia			56580		Ceriodaphnia			124443		Stenocypris			173493		Crangonyx			206734			Lampsilis			17696		Ceriodaphnia			20068		Hyalella			29092		Salmo			41264
	Site 15		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 16		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 17		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 18		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 19		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 20		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370

Overview

Background 2017 Recommended Criteria Status

3

Sources of Aluminumbull Aluminum is found in most rocks and

minerals and can enter the environment through natural weathering

bull Aluminumrsquos solubility increase under acidic (pH lt6) or basic (pH gt8) water conditions

bull Aluminum is actively mined from bauxite used in industrial processes and alum (potassium aluminum sulfate) is used in the clarification process at drinking water facilities 4

Current 1988 Aluminum Criteriabull The current 1988 criteria account for the

ndash Influence of pH on toxicity (solely by limiting criteria application range)

ndash Similarity of acute toxicity of fish and invertebrates

ndash The greater sensitivity of invertebrates in chronic toxicity tests

5

Current 1988 Aluminum Criteriabull The final 1988 criteria document recommended

ndash An acute criterion of 750 microgL total aluminum when the pH is between 65 and 90

ndash Chronic criterion of 87 microgL total aluminum when the pH is between 65 and 90

ndash The 1988 freshwater chronic criterion of 87 microgL was lowered based on two tests (brook trout and striped bass)

ndash The studies were not pH adjusted and all the data were not normalized for hardness The 1988 existing criteria did not take hardness and DOC into account

6

Aluminum Standardsbull Aluminum Standards

ndash About 20 states have numeric criteria for aluminum for aquatic life

bull Most are based on EPArsquos 1988 values and two are hardness-based equations (CO and NM)

bull There are numerous site-specific criteria that involve the Biotic Ligand Model (BLM) or a Water Effects Ratio (WER) -adjusted 1988 aluminum criteria

ndash Aluminum is not a priority pollutant

7

Criteria Development Processbull All EPA Aquatic Life Criteria for Toxics undergo rigorous

scientific development and review processbull Develop criteria document draftbull Intra-agency peer review process bull Independent external peer reviewbull Revisions based on external peer review

Intra-agency review of draft criteria documentbull Release to the public in draft form to obtain scientific views

Revisions considering public comments Intra-agency review of final criteria document

bull Publication of final criteria document bull States may then consider recommended criteria in

development and adoption of state water quality standards8

Framework for Updated Draft Aluminum Criteria

I Screen toxicological studies for high

quality data

II Consider water conditions effects on

aluminum bioavailability

III Normalize toxicity data in species

sensitivity distribution to a set of water quality conditions

IV Construct sensitivity distribution to determine criteria

at set conditions

9

I Screen toxicological studies for high quality data

bull First step in all criteria developmentndash Data are evaluated based on the data quality objectives

outlined in the 1985 Guidelines methodology and other toxicity testing recommendations (ie EPA ASTM)

ndash High quality data are used quantitatively to develop criteria magnitudes

ndash Lower quality data are used qualitatively if appropriatendash Lowest quality data are considered unacceptable and not

usedbull Data are grouped into acute and chronic exposures

10

Aluminum Toxicity Databull The updated freshwater aluminum criteria meets the

acute and chronic minimum data requirements outlined in EPArsquos 1985 Guidelines

InvertebrateSpecies

Fish Species

AmphibianSpecies

Total Genera

Acute1988 AWQC 8 7 - 142017 Draft AWQC 11 8 1 18

Chronic1988 AWQC 2 1 - 32017 Draft AWQC 7 4 - 11

11 1988 AWQC Chronic CCC based on two studies for the striped

bass and brook trout

Aluminum Toxicity Data Freshwater mussels have been

suggested to be potentially sensitive to aluminum

EPA worked collaboratively with USGSrsquos Columbia Environmental Research Center (CERC) to have acute and chronic aluminum toxicity tests conducted with a unionid mussel (fatmucket Lampsilis siliquoidea) in water-only exposures to obtain additional data on invertebrates New data on amphipod (Hyalella azteca)

were collected to confirm laboratory conditions

12

Aluminum Toxicity Data

13

bull New data and models examinedndash Data submitted to EU REACH program

bull New freshwater aluminum toxicity data generated by Oregon State University

bull 2016 BLM model provided by the European Aluminum Association

ndash Saltwater data provided by Australia bull Insufficient data to develop estuarinemarine acute or chronic

criteria at this timendash Multiple Linear Regression (MLR) model developed by

DeForest et al 2017bull DeForest DK KV Brix LM Tear and WJ Adams 2017 Multiple Linear Regression (MLR) models for predicting chronic aluminum

toxicity to freshwater aquatic organisms and developing water quality guidelines Environ Toxicol Chem (DOI101002etc3922)

14

Minimum Dataset For Freshwater Criteria Derivation

SALMONID SECONDFISHFAMILY

CHORDATA

PLANKTONICCRUSTACEAN

BENTHIC CRUSTACEAN

INSECT ROTIFERA ANNELIDA MOLLUSCA

OTHERINSECT ORMOLLUSCA

EstuarineMarine Minimum Data Requirements

15

Family Minimum Data Requirement EstuarineMarine

Acute(Phylum Family Genus)

Chronic(Phylum Family Genus)

Family in the phylum Chordata - -

Family in the phylum Chordata - -

Either the Mysidae or Penaeidae family - -

Family in a phylum other than Arthropoda or Chordata Mollusca Ostreidae Crassostrea -

Family in a phylum other than Chordata Annelida Nereididae Neanthes -

Family in a phylum other than Chordata Annelida Capitellidae Capitella -

Family in a phylum other than Chordata Annelida Ctenodrilidae Ctenodrilus -

Any other family Arthropoda Ameiridae Nitokra -

II Evaluate water quality conditions to determine applicability

bull Criteria are often based on one or more water quality conditions (eg hardness) that affect toxicity of the material to test organisms

bull Aluminum toxicity is highly dependent on the pH of the test waterndash The pH of test waters affects the amount and form

of aluminum that is bioavailable to test speciesndash Hardness and DOC (Dissolved Organic Carbon)

also influence the amount of aluminum that is bioavailable

16

Multiple Bioavailability Modeling Approaches Underwent External Peer Review

17

bull Biotic Ligand Modelsbull Full aluminum BLM (2016 model version) ndash Temperature pH

Dissolved organic carbon (DOC) Major cations (calcium [Ca] magnesium [Mg] sodium [Na] and potassium [K]) Major anions (sulfate [SO4] and chloride [Cl]) Alkalinity and Sulfide

bull Simplified aluminum BLM - pH hardness DOC temperature

bull Multilinear Regression Equationsbull pH and Hardness

Accounting for Effects of Water Chemistry on Bioavailability and Toxicity

bull Water quality criteria can be modified quantitatively when enough data are available to demonstrate that those conditions similarly affect the results with a variety of speciesndash High quality data in the draft aluminum document demonstrated

that pH DOC and hardness affected a variety of species in a similar way when exposed to aluminum concentrations

bull Recent publications by Cardwell et al (2017) and Gensemer et al (2017) summarized short-term aluminum chronic toxicity data across a range of DOC pH and hardness values for three species

18

Multiple Linear Regression (MLR) Models to Account for Effects of Water Chemistry

bull MLR models were developed for each species (DeForest et al 2017)ndash 119862119862 119889119889119906119906119887119887119894119894119886119886 11986411986411986211986220=119890119890[minus41026+[0525timesln(119863119863119874119874119862119862)]+[2201timesln(ℎ119886119886119903119903119889119889)]+(11282times119901119901119867119867)minus(0633times1199011199011198671198672)minus[0264times119901119901119867119867ln(ℎ119886119886119903119903119889119889)]]

ndash 119875119875 119901119901119903119903119900119900119898119898119890119890119897119897119886119886119904119904 11986411986411986211986220=119890119890[minus14029+[0503timesln(119863119863119874119874119862119862)]+[3443timesln(ℎ119886119886119903119903119889119889)]+(3131times119901119901119867119867)minus[0494times119901119901119867119867ln(ℎ119886119886119903119903119889119889)]]

bull These models were applied to both the acute and chronic aluminum toxicity datandash The C dubia model was applied to invertebrate datandash The P promelas model was applied to vertebrate data

19

III Normalize toxicity data in species sensitivity distribution to a set of water

quality conditionsbull Just like single parameter hardness models the MLR

models were used to normalize the aluminum toxicity data to a single set of water quality conditionsndash As an illustration for one set of calculations the draft

aluminum AWQC set these conditions to pH 7 hardness of 100 mgL and DOC of 1 mgL

bull This allows for comparisons to be made between species based on their sensitivity to aluminumndash Apples to apples

20

IV Construct sensitivity distribution to determine criteria at set conditions

21

Figure 8 Ranked Summary of Total Aluminum Genus Mean Acute Values (GMAVs) - Freshwater at pH 7 Hardness of 100 mgL and DOC of 1 mgL

IV Construct sensitivity distribution to determine criteria at set conditions

22

Figure 10 Ranked Summary of Total Aluminum Genus Mean Chronic Values (GMCVs) ndash Freshwater Supplemented with Other Data to Fulfill Missing MDRs at pH 7 Hardness of 100 mgL and DOC of 1 mgL

Aluminum Criteria Calculatorbull The relative ranking of genera in the sensitivity

distribution to which the toxicity data are normalized will change based on the set of water chemistry conditions (ie pH hardness and DOC)

bull To make calculating aluminum criteria easier EPA developed an Aluminum Criteria Calculatorndash Automated so that user only needs to enter water chemistry

conditions of interestndash Allows quick calculations for a variety of conditionsndash Limits calculations to the recommended conditions for which

the MLR models were developed were specified for hardness and DOC

bull No extrapolation beyond hardness and DOC empirical data bounds

23

Aluminum Criteria Calculator SD Example

24

pH=7 Hardness=100 DOC=1 pH=6 Hardness=25 DOC=1

Chronic Ranked GMCVRank GMCV Genus

12 17098 Aeolosoma Invert11 gt 9746 Rana Amphib - Other Data10 5908 Chironomus Invert

9 4877 Lymnaea Mollusk8 2555 Brachionus Invert7 2488 Pimephales Fish6 1713 Hyalella Invert5 1182 Ceriodaphnia Invert4 1102 Danio Fish3 1042 Lampsilis Mollusk2 591 Salvelinus Fish1 509 Salmo Fish

FCV = 3940 microgL total aluminum

Chronic Ranked GMCVRank GMCV Genus

12 gt 2131 Rana Amphib - Other Data11 1710 Aeolosoma Invert10 591 Chironomus Invert

9 544 Pimephales Fish8 488 Lymnaea Mollusk7 255 Brachionus Invert6 241 Danio Fish5 171 Hyalella Invert4 129 Salvelinus Fish3 118 Ceriodaphnia Invert2 111 Salmo Fish1 104 Lampsilis Mollusk

FCV = 987 microgL total aluminum

Multiple Scenarios

Run Scenarios

Summary Sheet

Acute Dataset

Chronic Dataset

Multiple Scenarios

Run Scenarios

Summary Sheet

Acute Dataset

Chronic Dataset

Boundaries of the Criteria Outputsbull MLR models were developed using data that

encompassndash DOC range of 008-5 mgLndash Hardness range of 98-127 mgL (as CaCO3)ndash pH range of 6-81

bull MLR criteria outputs are bounded atndash Maximum of 150 mgL total hardness (as CaCO3)

ndash DOC of 50 mgLndash pH range of the model is 50 to 90

25

Aluminum Criteria Look-Up Tables

26

bull EPA also provided criteria look-up tables in Appendix K

Har

dnes

s

CCC(microgL total aluminum)

(DOC=05 mgL)

pH 50 pH 55 pH 60 pH 65 pH 70 pH 75 pH 80 pH 85 pH 90

25 23 a 14 b 69 c 160 f 350 f 620 i 540 b 410 a 230 a

50 43 a 24 b 100 d 170 f 310 f 630 f 670 j 400 a 200 a

75 61 a 33 b 130 e 180 f 290 f 530 f 730 i 390 a 190 a

100 78 a 41 b 150 e 190 f 280 g 460 f 770 c 390 a 180 a

150 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

200 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

250 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

300 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

350 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

400 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

Aluminum Criteria Look-Up Tables

27

bull EPA also provided criteria look-up tables in Appendix K

Har

dnes

s

CCC(microgL total aluminum)

(DOC=10 mgL)

pH 50 pH 55 pH 60 pH 65 pH 70 pH 75 pH 80 pH 85 pH 90

25 33 a 21 b 99 c 220 f 490 f 900 i 780 b 590 a 330 a

50 61 a 35 b 150 e 250 f 440 f 890 f 960 j 580 a 290 a

75 88 a 47 b 180 e 260 f 410 f 740 f 1100 i 570 a 270 a

100 11 a 59 b 210 e 270 f 390 g 650 f 1100 c 560 b 260 a

150 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

200 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

250 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

300 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

350 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

400 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

Aluminum Criteria Look-Up Tables

28

bull EPA also provided criteria look-up tables in Appendix K

Har

dnes

s

CCC(microgL total aluminum)

(DOC=25 mgL)

pH 50 pH 55 pH 60 pH 65 pH 70 pH 75 pH 80 pH 85 pH 90

25 54 a 34 b 160 c 350 f 770 f 1500 i 1300 b 960 a 540 a

50 99 a 57 b 230 e 390 f 690 f 1400 f 1600 j 930 a 470 a

75 14 a 77 b 290 e 410 f 640 f 1200 f 1700 i 920 a 440 a

100 18 a 96 b 330 e 420 f 620 g 1000 f 1800 d 920 b 420 a

150 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

200 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

250 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

300 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

350 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

400 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

Aluminum Criteria Look-Up Tables

29

bull EPA also provided criteria look-up tables in Appendix K

Har

dnes

s

CCC(microgL total aluminum)

(DOC=50 mgL)

pH 50 pH 55 pH 60 pH 65 pH 70 pH 75 pH 80 pH 85 pH 90

25 77 a 49 b 230 c 500 f 1100 f 2100 c 1900 b 1400 a 770 a

50 14 a 82 b 330 e 540 f 970 f 2000 f 2200 j 1300 a 680 a

75 20 a 110 b 410 e 570 f 900 f 1600 f 2500 i 1300 a 640 a

100 26 a 140 b 460 f 590 f 880 g 1400 f 2500 d 1300 b 600 a

150 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

200 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

250 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

300 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

350 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

400 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

Status

30

bull Draft Document Released to the Federal Register on 72817ndash Public comment period of 60 days ndash Due Date for Comments is 92617

bull Revisions will be made considering public commentsbull Subsequent EPA intra-agency review of final criteria

document will then be conducted bull Publication of final criteria document planned for Late Fall

2017

Federal Register Webpagehttpswwwfederalregistergovdocuments201

707282017-15968request-for-scientific-views-draft-updated-aquatic-life-ambient-water-quality-criteria-for-aluminum

Aluminum Criteria Webpagehttpswwwepagovwqc2017-draft-aquatic-life-

criteria-aluminum-freshwater-documents

31

	Appendix C Acceptable Chronic Toxicity Data of Aluminum to Freshwater Aquatic Animals
									MLR equation
	Species		Hardness (mgL as CaCO3)		pH		DOC(mgL)		EC20 Endpoint			EC20(microgL)			Normalized Conc- EC20(microgL)			SMCV-EC20(microgL)		GMCV-EC20(microgL)			Genus			DOC Notes		Dilution water
	Oligochaete Aeolosoma sp		48		6		025		Reproduction (population size)			1235			1710			1710		1710			Aeolosoma		Invert		Author reported half the detection limit
	Rotifer Brachionus calyciflorus		100		62		025		Reproduction (population size)			431			255			255		255			Brachionus		Invert		Author reported half the detection limit
	Great pond snail Lymnaea stagnalis		117		61		025		Biomass			746			488			488		488			Lymnaea		Mollusk		Author reported half the detection limit
	Fatmucket Lampsilis siliquoidea		1055		604		040		Biomass			169			104			104		104			Lampsilis		Mollusk		Author reported
	Cladoceran Ceriodaphnia dubia		50		715		11		Reproduction - youngstarting adult			1780			190						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		26		73		05		Reproduction - youngfemale			1557			272						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		46		75		05		Reproduction - youngfemale			8087			110						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		96		79		05		Reproduction - youngfemale			6519			80						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		194		81		05		Reproduction - youngfemale			6836			89						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		255		703		05		Reproduction (youngfemale)			250			56						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		122		713		05		Reproduction (youngfemale)			860			106						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		25		798		05		Reproduction (youngfemale)			700			101						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		61		803		05		Reproduction (youngfemale)			1010			136						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		122		810		05		Reproduction (youngfemale)			870			116						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		255		633		05		Reproduction (youngfemale)			260			176						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		121		636		05		Reproduction (youngfemale)			390			110			118		118			Ceriodaphnia		Invert		Author reported
	Amphipod Hyalella azteca		95		61		051		Biomass (28 d)			1993			101						Hyalella			Author reported
	Amphipod Hyalella azteca		106		604		033		Biomass (28 d)			425			289			171		171			Hyalella		Invert		Author reported
	Midge Chironomus riparius		118		558		18		Adult midge emergence			2955			119						Chironomus			Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Midge Chironomus riparius		119		505		18		Adult midge emergence			8442			2258						Chironomus			Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Midge Chironomus riparius		91		66		051		Reproduction ( of eggscase)			3387			766			591		591			Chironomus		Invert		Author reported
	Atlantic salmon Salmo salar		127		57		18		Biomass			6156			1112			1112		1112			Salmo		Fish		Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Brook trout Salvelinus fontinalis		123		655		19		Biomass			1644			59						Salvelinus			Author reported		wellRO mix
	Brook trout Salvelinus fontinalis		128		565		18		Biomass			1435			283			1293		1293			Salvelinus		Fish		Author reported		wellRO mix
	Fathead minnow Pimephales promelas		220		770		16		Biomass			6194			781						Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		well water
	Fathead minnow Pimephales promelas		96		620		025		Fry survival			4286			379			544		544			Pimephales		Fish		Author reported half the detection limit
	Zebrafish Danio rerio		83		61		025		Biomass			2344			241			241		241			Danio		Fish		Author reported half the detection limit
	Wood frog Rana sylvatica		115		469		16		MATC-survival		gt		2000		gt		2131		gt		2131		2131		gt		Rana		Amphib - Other Data		Value is from 2007 FW Cu AWQC Appendix C recommendation		aged tap water

	Appendix A Acceptable Acute Toxicity Data of Aluminum to Freshwater Aquatic Animals
	Species		Methoda		Hardness (mgL as CaCO3)		pH		DOC(mgL)			LC50 or EC50 Conc (microgL)		Reference			Normalized Conc - LC50(microgL)			Species Mean Acute Value(microgL)		Genus Mean Acute Value(microgL)			Genus			DOC Note		Dilution water
	Snail Physa sp		S M T		474		659		11		gt		23400		Call 1984 Call et al 1984		gt		4843			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		755		11			30600		Call 1984 Call et al 1984			2699			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		817		11		gt		24700		Call 1984 Call et al 1984		gt		2404			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		746		11			55500		Call 1984 Call et al 1984			5034			3546		3546			Physa		Mollusk		Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Fatmucket Lampsilis siliquoidea		F M T		1060		612		048		gt		6302		Wang et al 2016 Wang et al 2017		gt		2983		gt		2983		2983		gt		Lampsilis		Mollusk		Author reported
	Cladoceran Ceriodaphnia dubia		S M A		500		742		11			1900		McCauley et al 1986			173			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		S M A		505		786		11			1500		McCauley et al 1986			129			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		UW-S Lake Superior lab water
	Cladoceran Ceriodaphnia dubia		S M A		500		813		11			2560		McCauley et al 1986			243			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		R M T		26		75		05			720		ENSR 1992d			111			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		R M T		46		76		05			1880		ENSR 1992d			250			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		R M T		96		78		05			2450		ENSR 1992d			289			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		S U NR		90		715		05			3727		Fort and Stover 1995			500			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U NR		90		715		05			5673		Fort and Stover 1995			761			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U NR		89		76		05			2880		Soucek et al 2001			336			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		601		05			7112		European Al Association 2009			169			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		605		2			6865		European Al Association 2009			709			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		609		4			15581		European Al Association 2009			1007			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		601		05			681		European Al Association 2009			162			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		603		05			1630		European Al Association 2009			367			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		597		05			1785		European Al Association 2009			473			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		592		05			1410		European Al Association 2009			429			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		699		05		gt		1300		European Al Association 2009		gt		419			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		785		05		gt		5000		European Al Association 2009		gt		796			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		68		2		gt		10000		European Al Association 2009		gt		2076			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		782		2		gt		15000		European Al Association 2009		gt		1163			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		677		4		gt		10000		European Al Association 2009		gt		1516			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		766		4		gt		15000		European Al Association 2009		gt		861			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		79		05		gt		2000		European Al Association 2009		gt		315			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		789		05		gt		2000		European Al Association 2009		gt		316			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		604		05			1108		European Al Association 2009			85			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		598		2			11371		European Al Association 2009			482			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		573		4			80467		European Al Association 2009			4357			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		671		05		gt		10000		European Al Association 2009		gt		2379			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		783		05		gt		5000		European Al Association 2009		gt		634			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		679		2		gt		10000		European Al Association 2009		gt		1039			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		767		2		gt		15000		European Al Association 2009		gt		908			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		668		4		gt		15000		European Al Association 2009		gt		1246			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		762		4		gt		15000		European Al Association 2009		gt		633			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		606		2			33868		European Al Association 2009			792			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		56		4			104842		European Al Association 2009			4880			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		693		05		gt		5000		European Al Association 2009		gt		713			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		120		788		05		gt		5000		European Al Association 2009		gt		590			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		120		676		2		gt		15000		European Al Association 2009		gt		1212			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		771		2		gt		15000		European Al Association 2009		gt		810			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		66		4		gt		15000		European Al Association 2009		gt		1014			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		76		4		gt		15000		European Al Association 2009		gt		555			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			11971		European Al Association 2010			270			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			27478		European Al Association 2010			619			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			11998		European Al Association 2010			270			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		607		05			92495		European Al Association 2010			188			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		609		05			31337		European Al Association 2010			604			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		61		05			33235		European Al Association 2010			624			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		708		05		gt		8864		European Al Association 2010		gt		253			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		779		05		gt		42783		European Al Association 2010		gt		693			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		753		05			13204		European Al Association 2010			24			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		600		601		05			46326		European Al Association 2010			380			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		600		599		05		gt		8590		European Al Association 2010		gt		737			509				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia reticulata		F M T		451		60		11			304		Shephard 1983			201			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia reticulata		F M T		40		55		11			362		Shephard 1983			5531			1054		733			Ceriodaphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake SuperiorDI Mix
	Cladoceran Daphnia magna		S U NR		453		774		11			3900		Biesinger and Christensen 1972			338			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Daphnia magna		S M T		220		76		16			38200		Kimball 1978			2030			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Well water
	Cladoceran Daphnia magna		S U T		451		725		11			2800		Shephard 1983			287			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Daphnia magna		S U T		168		599		05			5000		European Al Association 2009		gt		227			-				Daphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Daphnia magna		S U T		168		792		05			7950		European Al Association 2009			92			333		333			Daphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Ostracod Stenocypris major		R M T		1563		651		16			310196		Shuhaimi-Othman et al 2011a			1022			1022		1022			Stenocypris		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (Malaysia)
	Amphipod Crangonyx pseudogracilis		R U T		50		675		16			9190		Martin and Holdich 1986			1217			1217		1217			Crangonyx		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (UK)
	Amphipod Hyalella azteca		F M T		105		613		048		gt		5997		Wang et al 2016 Wang et al 2017		gt		2800		gt		2800		2800		gt		Hyalella		Invert		Author reported
	Stonefly Acroneuria sp		S M T		474		746		11		gt		22600		Call et al 1984		gt		2050		gt		2050		2050		gt		Acroneuria		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Midge Chironomus plumosus		S U T		80		70		16			30000		Fargasova 2001			2536			2536		2536			Chironomus		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (Slovakia)
	Midge Paratanytarsus dissimilis		S M T		1743		728		28		gt		77700		Lamb and Bailey 1981 1983		gt		6232		gt		6232		6232		gt		Paratanytarsus		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Liberty Lake Washington
	Rainbow trout Oncorhynchus mykiss		F M T		274		758		05		gt		9840		Gundersen et al 1994		gt		1256			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		452		762		05		gt		8070		Gundersen et al 1994		gt		1140			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		895		758		05		gt		8160		Gundersen et al 1994		gt		1489			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		1304		758		05		gt		8200		Gundersen et al 1994		gt		1676			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		232		825		05			6170		Gundersen et al 1994			260			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		350		826		05			6170		Gundersen et al 1994			333			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		836		829		05			7670		Gundersen et al 1994			701			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		1158		830		05			6930		Gundersen et al 1994			777			801		801			Oncorhynchus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Atlantic salmon Salmo salar		S U T		68		55		05			584		Hamilton and Haines 1995			4602			-				Salmo			Value is from 2007 FW Cu AWQC Appendix C recommendation		Soft recon
	Atlantic salmon Salmo salar		S U T		68		65		05			599		Hamilton and Haines 1995			531			1564		1564			Salmo		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Soft recon
	Brook trout Salvelinus fontinalis		S U T		40		56		16			6530		Tandjung 1982			6947			-				Salvelinus			Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Brook trout Salvelinus fontinalis		S U T		18		56		16			3400		Tandjung 1982			6209			-				Salvelinus			Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Brook trout Salvelinus fontinalis		S U T		2		56		16			370		Tandjung 1982			2988			5051		5051			Salvelinus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Green sunfish Lepomis cyanellus		S M T		474		755		11		gt		50000		Call et al 1984		gt		5255		gt		5255		5255		gt		Lepomis		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Rio Grande silvery minnow Hybognathus amarus		R M T		140		81		05		gt		59100		Buhl 2002		gt		8620		gt		8620		8620		gt		Hybognathus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon water
	Fathead minnow Pimephales promelas		S M T		474		761		11		gt		48200		Call et al 1984		gt		4707			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Fathead minnow Pimephales promelas		S M T		474		805		11		gt		49800		Call et al 1984		gt		2837			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Fathead minnow Pimephales promelas		R M T		26		78		05			1160		ENSR 1992c			104			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		46		76		05			8180		ENSR 1992c			1191			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		96		81		05			20300		ENSR 1992c			2398			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		194		81		05			44800		ENSR 1992c			7839			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		140		81		05		gt		59100		Buhl 2002		gt		8620			2224		2224			Pimephales		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon water
	Smallmouth bass Micropterus dolomieui		S M T		1245		505		16			130		Kane and Rabeni 1987			859			859		859			Micropterus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Green tree frog Hyla cinerea		R M T		455		549		05		gt		4052		Jung and Jagoe 1995		gt		4377		gt		4377		4377		gt		Hyla		Amphibian		Value is from 2007 FW Cu AWQC Appendix C recommendation		artificial soft recon

	Normalization Chemistry
	pH		6					FAV		360
	Hardness		25					CMC		180
	DOC		1					CCC		99
	Acute Ranked GMAV											Chronic Ranked GMCV
	Rank			GMAV		Genus								Rank			GMCV		Genus
	18		gt		8620		Hybognathus		Fish							12		gt		2131		Rana		Amphib - Other Data
	17		gt		6232		Paratanytarsus		Invert							11			1710		Aeolosoma		Invert
	16		gt		5255		Lepomis		Fish							10			591		Chironomus		Invert
	15			5051		Salvelinus		Fish							9			544		Pimephales		Fish
	14		gt		4377		Hyla		Amphibian							8			488		Lymnaea		Mollusk
	13			3546		Physa		Mollusk							7			255		Brachionus		Invert
	12		gt		2983		Lampsilis		Mollusk							6			241		Danio		Fish
	11		gt		2800		Hyalella		Invert							5			171		Hyalella		Invert
	10			2536		Chironomus		Invert							4			129		Salvelinus		Fish
	9			2224		Pimephales		Fish							3			118		Ceriodaphnia		Invert
	8		gt		2050		Acroneuria		Invert							2			111		Salmo		Fish
	7			1564		Salmo		Fish							1			104		Lampsilis		Mollusk
	6			1217		Crangonyx		Invert
	5			1022		Stenocypris		Invert
	4			859		Micropterus		Fish
	3			801		Oncorhynchus		Fish
	2			733		Ceriodaphnia		Invert
	1			333		Daphnia		Invert
		N		Rank		Genus		GMAV		ln(GMAV)		ln(GMAV)2		P=R(N+1)		sqrt(P)				N		Rank		Genus		GMCV		ln(GMCV)		ln(GMCV)2		P=R(N+1)		sqrt(P)
		18		4		Micropterus		859		676		4564		0211		0459				12		4		Salvelinus		129		486		2364		0308		0555
			3		Oncorhynchus		801		669		4469		0158		0397					3		Ceriodaphnia		118		477		2277		0231		0480
			2		Ceriodaphnia		733		660		4352		0105		0324					2		Salmo		111		471		2220		0154		0392
			1		Daphnia		333		581		3374		0053		0229					1		Lampsilis		104		465		2159		0077		0277
			Sum				2585		1676		0526		141					Sum				1899		902		0769		170
			S2 =		1987										S2 =		059
			L =		4890										L =		4420
			A =		5887										A =		4592
			FAV =		360										FCV =		987
			CMC =		180

				All concentrations reported are microgL total Aluminum
				lt-----Click Button after entering data
	ENTER DATA HERE									ERRORNUM		ACUTE														CHRONIC
										Rank 1				Rank 2				Rank 3				Rank 4					Rank 1				Rank 2				Rank 3				Rank 4
	SiteName		DOC(mgL)		Hardness(mgL as CaCO3)		pH			FAV		CMC		CCC			Genus		GMAV			Genus		GMAV			Genus		GMAV			Genus		GMAV				Genus		GMCV			Genus		GMCV			Genus		GMCV			Genus		GMCV
	Site 1		50		25		9		75428737034162		35229324666008		1800		770			Daphnia			33452		Ceriodaphnia			73576		Stenocypris			102576		Crangonyx			122230			Lampsilis			10463		Ceriodaphnia			11865		Hyalella			17200		Brachionus			25648
	Site 2		50		50		9		75428737034162		31204991509063		1600		680			Daphnia			29631		Ceriodaphnia			65171		Stenocypris			90859		Crangonyx			108267			Lampsilis			9268		Ceriodaphnia			10510		Hyalella			15236		Brachionus			22718
	Site 3		50		75		9		75428737034162		29067527928199		1500		640			Daphnia			27601		Ceriodaphnia			60707		Stenocypris			84635		Crangonyx			100851			Lampsilis			8633		Ceriodaphnia			9790		Hyalella			14192		Brachionus			21162
	Site 4		50		100		9		75428737034162		27640367912592		1400		600			Daphnia			26246		Ceriodaphnia			57726		Stenocypris			80480		Crangonyx			95899			Lampsilis			8209		Ceriodaphnia			9309		Hyalella			13495		Brachionus			20123
	Site 5		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 6		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 7		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 8		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 9		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 10		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 11		50		25		85		75428737034162		63245275664384		3200		1400			Daphnia			60055		Ceriodaphnia			132086		Stenocypris			184149		Crangonyx			219432			Lampsilis			18783		Ceriodaphnia			21301		Hyalella			30879		Brachionus			46045
	Site 12		50		50		85		75428737034162		61388044609926		3100		1300			Daphnia			58291		Ceriodaphnia			128208		Stenocypris			178742		Crangonyx			212988			Lampsilis			18232		Ceriodaphnia			20676		Hyalella			29972		Brachionus			44693
	Site 13		50		75		85		75428737034162		60327020429132		3000		1300			Daphnia			57284		Ceriodaphnia			125992		Stenocypris			175652		Crangonyx			209307			Lampsilis			17917		Ceriodaphnia			20318		Hyalella			29454		Brachionus			43920
	Site 14		50		100		85		75428737034162		59585352130146		3000		1300			Daphnia			56580		Ceriodaphnia			124443		Stenocypris			173493		Crangonyx			206734			Lampsilis			17696		Ceriodaphnia			20068		Hyalella			29092		Salmo			41264
	Site 15		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 16		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 17		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 18		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 19		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 20		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370

	Appendix C Acceptable Chronic Toxicity Data of Aluminum to Freshwater Aquatic Animals
									MLR equation
	Species		Hardness (mgL as CaCO3)		pH		DOC(mgL)		EC20 Endpoint			EC20(microgL)			Normalized Conc- EC20(microgL)			SMCV-EC20(microgL)		GMCV-EC20(microgL)			Genus			DOC Notes		Dilution water
	Oligochaete Aeolosoma sp		48		6		025		Reproduction (population size)			1235			17098			17098		17098			Aeolosoma		Invert		Author reported half the detection limit
	Rotifer Brachionus calyciflorus		100		62		025		Reproduction (population size)			431			2555			2555		2555			Brachionus		Invert		Author reported half the detection limit
	Great pond snail Lymnaea stagnalis		117		61		025		Biomass			746			4877			4877		4877			Lymnaea		Mollusk		Author reported half the detection limit
	Fatmucket Lampsilis siliquoidea		1055		604		040		Biomass			169			1042			1042		1042			Lampsilis		Mollusk		Author reported
	Cladoceran Ceriodaphnia dubia		50		715		11		Reproduction - youngstarting adult			1780			1898						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		26		73		05		Reproduction - youngfemale			1557			2718						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		46		75		05		Reproduction - youngfemale			8087			1102						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		96		79		05		Reproduction - youngfemale			6519			796						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		194		81		05		Reproduction - youngfemale			6836			888						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		255		703		05		Reproduction (youngfemale)			250			563						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		122		713		05		Reproduction (youngfemale)			860			1061						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		25		798		05		Reproduction (youngfemale)			700			1006						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		61		803		05		Reproduction (youngfemale)			1010			1363						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		122		810		05		Reproduction (youngfemale)			870			1164						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		255		633		05		Reproduction (youngfemale)			260			1758						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		121		636		05		Reproduction (youngfemale)			390			1101			1182		1182			Ceriodaphnia		Invert		Author reported
	Amphipod Hyalella azteca		95		61		051		Biomass (28 d)			1993			1014						Hyalella			Author reported
	Amphipod Hyalella azteca		106		604		033		Biomass (28 d)			425			2895			1713		1713			Hyalella		Invert		Author reported
	Midge Chironomus riparius		118		558		18		Adult midge emergence			2955			1192						Chironomus			Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Midge Chironomus riparius		119		505		18		Adult midge emergence			8442			22578						Chironomus			Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Midge Chironomus riparius		91		66		051		Reproduction ( of eggscase)			3387			7664			5908		5908			Chironomus		Invert		Author reported
	Atlantic salmon Salmo salar		127		57		18		Biomass			6156			5085			5085		5085			Salmo		Fish		Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Brook trout Salvelinus fontinalis		123		655		19		Biomass			1644			270						Salvelinus			Author reported		wellRO mix
	Brook trout Salvelinus fontinalis		128		565		18		Biomass			1435			1295			5914		5914			Salvelinus		Fish		Author reported		wellRO mix
	Fathead minnow Pimephales promelas		220		770		16		Biomass			6194			3569						Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		well water
	Fathead minnow Pimephales promelas		96		620		025		Fry survival			4286			1734			2488		2488			Pimephales		Fish		Author reported half the detection limit
	Zebrafish Danio rerio		83		61		025		Biomass			2344			1102			1102		1102			Danio		Fish		Author reported half the detection limit
	Wood frog Rana sylvatica		115		469		16		MATC-survival		gt		2000		gt		9746		gt		9746		9746		gt		Rana		Amphib - Other Data		Value is from 2007 FW Cu AWQC Appendix C recommendation		aged tap water

	Appendix A Acceptable Acute Toxicity Data of Aluminum to Freshwater Aquatic Animals
	Species		Methoda		Hardness (mgL as CaCO3)		pH		DOC(mgL)			LC50 or EC50 Conc (microgL)		Reference			Normalized Conc - LC50(microgL)			Species Mean Acute Value(microgL)		Genus Mean Acute Value(microgL)			Genus			DOC Note		Dilution water
	Snail Physa sp		S M T		474		659		11		gt		23400		Call 1984 Call et al 1984		gt		48425			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		755		11			30600		Call 1984 Call et al 1984			26986			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		817		11		gt		24700		Call 1984 Call et al 1984		gt		24040			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		746		11			55500		Call 1984 Call et al 1984			50338			35462		35462			Physa		Mollusk		Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Fatmucket Lampsilis siliquoidea		F M T		1060		612		048		gt		6302		Wang et al 2016 Wang et al 2017		gt		29834		gt		29834		29834		gt		Lampsilis		Mollusk		Author reported
	Cladoceran Ceriodaphnia dubia		S M A		500		742		11			1900		McCauley et al 1986			1728			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		S M A		505		786		11			1500		McCauley et al 1986			1294			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		UW-S Lake Superior lab water
	Cladoceran Ceriodaphnia dubia		S M A		500		813		11			2560		McCauley et al 1986			2431			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		R M T		26		75		05			720		ENSR 1992d			1113			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		R M T		46		76		05			1880		ENSR 1992d			2495			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		R M T		96		78		05			2450		ENSR 1992d			2894			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		S U NR		90		715		05			3727		Fort and Stover 1995			5002			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U NR		90		715		05			5673		Fort and Stover 1995			7613			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U NR		89		76		05			2880		Soucek et al 2001			3362			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		601		05			7112		European Al Association 2009			1691			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		605		2			6865		European Al Association 2009			7088			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		609		4			15581		European Al Association 2009			10072			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		601		05			681		European Al Association 2009			1620			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		603		05			1630		European Al Association 2009			3674			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		597		05			1785		European Al Association 2009			4732			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		592		05			1410		European Al Association 2009			4295			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		699		05		gt		1300		European Al Association 2009		gt		4189			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		785		05		gt		5000		European Al Association 2009		gt		7960			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		68		2		gt		10000		European Al Association 2009		gt		20755			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		782		2		gt		15000		European Al Association 2009		gt		11626			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		677		4		gt		10000		European Al Association 2009		gt		15161			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		766		4		gt		15000		European Al Association 2009		gt		8608			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		79		05		gt		2000		European Al Association 2009		gt		3150			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		789		05		gt		2000		European Al Association 2009		gt		3156			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		604		05			1108		European Al Association 2009			850			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		598		2			11371		European Al Association 2009			4817			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		573		4			80467		European Al Association 2009			43570			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		671		05		gt		10000		European Al Association 2009		gt		23786			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		783		05		gt		5000		European Al Association 2009		gt		6344			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		679		2		gt		10000		European Al Association 2009		gt		10394			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		767		2		gt		15000		European Al Association 2009		gt		9082			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		668		4		gt		15000		European Al Association 2009		gt		12461			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		762		4		gt		15000		European Al Association 2009		gt		6332			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		606		2			33868		European Al Association 2009			7920			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		56		4			104842		European Al Association 2009			48800			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		693		05		gt		5000		European Al Association 2009		gt		7126			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		120		788		05		gt		5000		European Al Association 2009		gt		5898			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		120		676		2		gt		15000		European Al Association 2009		gt		12117			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		771		2		gt		15000		European Al Association 2009		gt		8096			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		66		4		gt		15000		European Al Association 2009		gt		10139			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		76		4		gt		15000		European Al Association 2009		gt		5545			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			11971		European Al Association 2010			2699			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			27478		European Al Association 2010			6194			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			11998		European Al Association 2010			2705			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		607		05			92495		European Al Association 2010			1876			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		609		05			31337		European Al Association 2010			6035			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		61		05			33235		European Al Association 2010			6238			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		708		05		gt		8864		European Al Association 2010		gt		2534			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		779		05		gt		42783		European Al Association 2010		gt		6930			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		753		05			13204		European Al Association 2010			244			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		600		601		05			46326		European Al Association 2010			3798			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		600		599		05		gt		8590		European Al Association 2010		gt		7366			5094				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia reticulata		F M T		451		60		11			304		Shephard 1983			2009			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia reticulata		F M T		40		55		11			362		Shephard 1983			55307			10542		7328			Ceriodaphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake SuperiorDI Mix
	Cladoceran Daphnia magna		S U NR		453		774		11			3900		Biesinger and Christensen 1972			3382			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Daphnia magna		S M T		220		76		16			38200		Kimball 1978			20303			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Well water
	Cladoceran Daphnia magna		S U T		451		725		11			2800		Shephard 1983			2868			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Daphnia magna		S U T		168		599		05			5000		European Al Association 2009		gt		2265			-				Daphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Daphnia magna		S U T		168		792		05			7950		European Al Association 2009			920			3332		3332			Daphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Ostracod Stenocypris major		R M T		1563		651		16			310196		Shuhaimi-Othman et al 2011a			10216			10216		10216			Stenocypris		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (Malaysia)
	Amphipod Crangonyx pseudogracilis		R U T		50		675		16			9190		Martin and Holdich 1986			12174			12174		12174			Crangonyx		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (UK)
	Amphipod Hyalella azteca		F M T		105		613		048		gt		5997		Wang et al 2016 Wang et al 2017		gt		28002		gt		28002		28002		gt		Hyalella		Invert		Author reported
	Stonefly Acroneuria sp		S M T		474		746		11		gt		22600		Call et al 1984		gt		20498		gt		20498		20498		gt		Acroneuria		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Midge Chironomus plumosus		S U T		80		70		16			30000		Fargasova 2001			25361			25361		25361			Chironomus		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (Slovakia)
	Midge Paratanytarsus dissimilis		S M T		1743		728		28		gt		77700		Lamb and Bailey 1981 1983		gt		62318		gt		62318		62318		gt		Paratanytarsus		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Liberty Lake Washington
	Rainbow trout Oncorhynchus mykiss		F M T		274		758		05		gt		9840		Gundersen et al 1994		gt		5745			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		452		762		05		gt		8070		Gundersen et al 1994		gt		5212			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		895		758		05		gt		8160		Gundersen et al 1994		gt		6807			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		1304		758		05		gt		8200		Gundersen et al 1994		gt		7662			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		232		825		05			6170		Gundersen et al 1994			1190			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		350		826		05			6170		Gundersen et al 1994			1523			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		836		829		05			7670		Gundersen et al 1994			3205			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		1158		830		05			6930		Gundersen et al 1994			3554			3661		3661			Oncorhynchus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Atlantic salmon Salmo salar		S U T		68		55		05			584		Hamilton and Haines 1995			21042			-				Salmo			Value is from 2007 FW Cu AWQC Appendix C recommendation		Soft recon
	Atlantic salmon Salmo salar		S U T		68		65		05			599		Hamilton and Haines 1995			2430			7151		7151			Salmo		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Soft recon
	Brook trout Salvelinus fontinalis		S U T		40		56		16			6530		Tandjung 1982			31767			-				Salvelinus			Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Brook trout Salvelinus fontinalis		S U T		18		56		16			3400		Tandjung 1982			28391			-				Salvelinus			Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Brook trout Salvelinus fontinalis		S U T		2		56		16			370		Tandjung 1982			13663			23097		23097			Salvelinus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Green sunfish Lepomis cyanellus		S M T		474		755		11		gt		50000		Call et al 1984		gt		24028		gt		24028		24028		gt		Lepomis		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Rio Grande silvery minnow Hybognathus amarus		R M T		140		81		05		gt		59100		Buhl 2002		gt		39414		gt		39414		39414		gt		Hybognathus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon water
	Fathead minnow Pimephales promelas		S M T		474		761		11		gt		48200		Call et al 1984		gt		21522			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Fathead minnow Pimephales promelas		S M T		474		805		11		gt		49800		Call et al 1984		gt		12972			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Fathead minnow Pimephales promelas		R M T		26		78		05			1160		ENSR 1992c			477			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		46		76		05			8180		ENSR 1992c			5446			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		96		81		05			20300		ENSR 1992c			10966			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		194		81		05			44800		ENSR 1992c			35847			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		140		81		05		gt		59100		Buhl 2002		gt		39414			10168		10168			Pimephales		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon water
	Smallmouth bass Micropterus dolomieui		S M T		1245		505		16			130		Kane and Rabeni 1987			3929			3929		3929			Micropterus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Green tree frog Hyla cinerea		R M T		455		549		05		gt		4052		Jung and Jagoe 1995		gt		20016		gt		20016		20016		gt		Hyla		Amphibian		Value is from 2007 FW Cu AWQC Appendix C recommendation		artificial soft recon

	Normalization Chemistry
	pH		7					FAV		2741
	Hardness		100					CMC		1400
	DOC		1					CCC		390
	Acute Ranked GMAV											Chronic Ranked GMCV
	Rank			GMAV		Genus								Rank			GMCV		Genus
	18		gt		62318		Paratanytarsus		Invert							12			17098		Aeolosoma		Invert
	17		gt		39414		Hybognathus		Fish							11		gt		9746		Rana		Amphib - Other Data
	16			35462		Physa		Mollusk							10			5908		Chironomus		Invert
	15		gt		29834		Lampsilis		Mollusk							9			4877		Lymnaea		Mollusk
	14		gt		28002		Hyalella		Invert							8			2555		Brachionus		Invert
	13			25361		Chironomus		Invert							7			2488		Pimephales		Fish
	12		gt		24028		Lepomis		Fish							6			1713		Hyalella		Invert
	11			23097		Salvelinus		Fish							5			1182		Ceriodaphnia		Invert
	10		gt		20498		Acroneuria		Invert							4			1102		Danio		Fish
	9		gt		20016		Hyla		Amphibian							3			1042		Lampsilis		Mollusk
	8			12174		Crangonyx		Invert							2			591		Salvelinus		Fish
	7			10216		Stenocypris		Invert							1			509		Salmo		Fish
	6			10168		Pimephales		Fish
	5			7328		Ceriodaphnia		Invert
	4			7151		Salmo		Fish
	3			3929		Micropterus		Fish
	2			3661		Oncorhynchus		Fish
	1			3332		Daphnia		Invert
		N		Rank		Genus		GMAV		ln(GMAV)		ln(GMAV)2		P=R(N+1)		sqrt(P)				N		Rank		Genus		GMCV		ln(GMCV)		ln(GMCV)2		P=R(N+1)		sqrt(P)
		18		4		Salmo		7151		887		7876		0211		0459				12		4		Danio		1102		700		4907		0308		0555
			3		Micropterus		3929		828		6849		0158		0397					3		Lampsilis		1042		695		4829		0231		0480
			2		Oncorhynchus		3661		821		6733		0105		0324					2		Salvelinus		591		638		4074		0154		0392
			1		Daphnia		3332		811		6579		0053		0229					1		Salmo		509		623		3883		0077		0277
			Sum				3347		2804		0526		141					Sum				2657		1769		0769		170
			S2 =		1223										S2 =		1080
			L =		7134										L =		5242
			A =		7916										A =		5976
			FAV =		2741										FCV =		3940
			CMC =		1371

				All concentrations reported are microgL total Aluminum
				lt-----Click Button after entering data
	ENTER DATA HERE									ERRORNUM		ACUTE														CHRONIC
										Rank 1				Rank 2				Rank 3				Rank 4					Rank 1				Rank 2				Rank 3				Rank 4
	SiteName		DOC(mgL)		Hardness(mgL as CaCO3)		pH			FAV		CMC		CCC			Genus		GMAV			Genus		GMAV			Genus		GMAV			Genus		GMAV				Genus		GMCV			Genus		GMCV			Genus		GMCV			Genus		GMCV
	Site 1		50		25		9		75428737034162		35229324666008		1800		770			Daphnia			33452		Ceriodaphnia			73576		Stenocypris			102576		Crangonyx			122230			Lampsilis			10463		Ceriodaphnia			11865		Hyalella			17200		Brachionus			25648
	Site 2		50		50		9		75428737034162		31204991509063		1600		680			Daphnia			29631		Ceriodaphnia			65171		Stenocypris			90859		Crangonyx			108267			Lampsilis			9268		Ceriodaphnia			10510		Hyalella			15236		Brachionus			22718
	Site 3		50		75		9		75428737034162		29067527928199		1500		640			Daphnia			27601		Ceriodaphnia			60707		Stenocypris			84635		Crangonyx			100851			Lampsilis			8633		Ceriodaphnia			9790		Hyalella			14192		Brachionus			21162
	Site 4		50		100		9		75428737034162		27640367912592		1400		600			Daphnia			26246		Ceriodaphnia			57726		Stenocypris			80480		Crangonyx			95899			Lampsilis			8209		Ceriodaphnia			9309		Hyalella			13495		Brachionus			20123
	Site 5		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 6		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 7		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 8		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 9		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 10		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 11		50		25		85		75428737034162		63245275664384		3200		1400			Daphnia			60055		Ceriodaphnia			132086		Stenocypris			184149		Crangonyx			219432			Lampsilis			18783		Ceriodaphnia			21301		Hyalella			30879		Brachionus			46045
	Site 12		50		50		85		75428737034162		61388044609926		3100		1300			Daphnia			58291		Ceriodaphnia			128208		Stenocypris			178742		Crangonyx			212988			Lampsilis			18232		Ceriodaphnia			20676		Hyalella			29972		Brachionus			44693
	Site 13		50		75		85		75428737034162		60327020429132		3000		1300			Daphnia			57284		Ceriodaphnia			125992		Stenocypris			175652		Crangonyx			209307			Lampsilis			17917		Ceriodaphnia			20318		Hyalella			29454		Brachionus			43920
	Site 14		50		100		85		75428737034162		59585352130146		3000		1300			Daphnia			56580		Ceriodaphnia			124443		Stenocypris			173493		Crangonyx			206734			Lampsilis			17696		Ceriodaphnia			20068		Hyalella			29092		Salmo			41264
	Site 15		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 16		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 17		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 18		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 19		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 20		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370

Sources of Aluminumbull Aluminum is found in most rocks and

minerals and can enter the environment through natural weathering

bull Aluminumrsquos solubility increase under acidic (pH lt6) or basic (pH gt8) water conditions

bull Aluminum is actively mined from bauxite used in industrial processes and alum (potassium aluminum sulfate) is used in the clarification process at drinking water facilities 4

Current 1988 Aluminum Criteriabull The current 1988 criteria account for the

ndash Influence of pH on toxicity (solely by limiting criteria application range)

ndash Similarity of acute toxicity of fish and invertebrates

ndash The greater sensitivity of invertebrates in chronic toxicity tests

5

Current 1988 Aluminum Criteriabull The final 1988 criteria document recommended

ndash An acute criterion of 750 microgL total aluminum when the pH is between 65 and 90

ndash Chronic criterion of 87 microgL total aluminum when the pH is between 65 and 90

ndash The 1988 freshwater chronic criterion of 87 microgL was lowered based on two tests (brook trout and striped bass)

ndash The studies were not pH adjusted and all the data were not normalized for hardness The 1988 existing criteria did not take hardness and DOC into account

6

Aluminum Standardsbull Aluminum Standards

ndash About 20 states have numeric criteria for aluminum for aquatic life

bull Most are based on EPArsquos 1988 values and two are hardness-based equations (CO and NM)

bull There are numerous site-specific criteria that involve the Biotic Ligand Model (BLM) or a Water Effects Ratio (WER) -adjusted 1988 aluminum criteria

ndash Aluminum is not a priority pollutant

7

Criteria Development Processbull All EPA Aquatic Life Criteria for Toxics undergo rigorous

scientific development and review processbull Develop criteria document draftbull Intra-agency peer review process bull Independent external peer reviewbull Revisions based on external peer review

Intra-agency review of draft criteria documentbull Release to the public in draft form to obtain scientific views

Revisions considering public comments Intra-agency review of final criteria document

bull Publication of final criteria document bull States may then consider recommended criteria in

development and adoption of state water quality standards8

Framework for Updated Draft Aluminum Criteria

I Screen toxicological studies for high

quality data

II Consider water conditions effects on

aluminum bioavailability

III Normalize toxicity data in species

sensitivity distribution to a set of water quality conditions

IV Construct sensitivity distribution to determine criteria

at set conditions

9

I Screen toxicological studies for high quality data

bull First step in all criteria developmentndash Data are evaluated based on the data quality objectives

outlined in the 1985 Guidelines methodology and other toxicity testing recommendations (ie EPA ASTM)

ndash High quality data are used quantitatively to develop criteria magnitudes

ndash Lower quality data are used qualitatively if appropriatendash Lowest quality data are considered unacceptable and not

usedbull Data are grouped into acute and chronic exposures

10

Aluminum Toxicity Databull The updated freshwater aluminum criteria meets the

acute and chronic minimum data requirements outlined in EPArsquos 1985 Guidelines

InvertebrateSpecies

Fish Species

AmphibianSpecies

Total Genera

Acute1988 AWQC 8 7 - 142017 Draft AWQC 11 8 1 18

Chronic1988 AWQC 2 1 - 32017 Draft AWQC 7 4 - 11

11 1988 AWQC Chronic CCC based on two studies for the striped

bass and brook trout

Aluminum Toxicity Data Freshwater mussels have been

suggested to be potentially sensitive to aluminum

EPA worked collaboratively with USGSrsquos Columbia Environmental Research Center (CERC) to have acute and chronic aluminum toxicity tests conducted with a unionid mussel (fatmucket Lampsilis siliquoidea) in water-only exposures to obtain additional data on invertebrates New data on amphipod (Hyalella azteca)

were collected to confirm laboratory conditions

12

Aluminum Toxicity Data

13

bull New data and models examinedndash Data submitted to EU REACH program

bull New freshwater aluminum toxicity data generated by Oregon State University

bull 2016 BLM model provided by the European Aluminum Association

ndash Saltwater data provided by Australia bull Insufficient data to develop estuarinemarine acute or chronic

criteria at this timendash Multiple Linear Regression (MLR) model developed by

DeForest et al 2017bull DeForest DK KV Brix LM Tear and WJ Adams 2017 Multiple Linear Regression (MLR) models for predicting chronic aluminum

toxicity to freshwater aquatic organisms and developing water quality guidelines Environ Toxicol Chem (DOI101002etc3922)

14

Minimum Dataset For Freshwater Criteria Derivation

SALMONID SECONDFISHFAMILY

CHORDATA

PLANKTONICCRUSTACEAN

BENTHIC CRUSTACEAN

INSECT ROTIFERA ANNELIDA MOLLUSCA

OTHERINSECT ORMOLLUSCA

EstuarineMarine Minimum Data Requirements

15

Family Minimum Data Requirement EstuarineMarine

Acute(Phylum Family Genus)

Chronic(Phylum Family Genus)

Family in the phylum Chordata - -

Family in the phylum Chordata - -

Either the Mysidae or Penaeidae family - -

Family in a phylum other than Arthropoda or Chordata Mollusca Ostreidae Crassostrea -

Family in a phylum other than Chordata Annelida Nereididae Neanthes -

Family in a phylum other than Chordata Annelida Capitellidae Capitella -

Family in a phylum other than Chordata Annelida Ctenodrilidae Ctenodrilus -

Any other family Arthropoda Ameiridae Nitokra -

II Evaluate water quality conditions to determine applicability

bull Criteria are often based on one or more water quality conditions (eg hardness) that affect toxicity of the material to test organisms

bull Aluminum toxicity is highly dependent on the pH of the test waterndash The pH of test waters affects the amount and form

of aluminum that is bioavailable to test speciesndash Hardness and DOC (Dissolved Organic Carbon)

also influence the amount of aluminum that is bioavailable

16

Multiple Bioavailability Modeling Approaches Underwent External Peer Review

17

bull Biotic Ligand Modelsbull Full aluminum BLM (2016 model version) ndash Temperature pH

Dissolved organic carbon (DOC) Major cations (calcium [Ca] magnesium [Mg] sodium [Na] and potassium [K]) Major anions (sulfate [SO4] and chloride [Cl]) Alkalinity and Sulfide

bull Simplified aluminum BLM - pH hardness DOC temperature

bull Multilinear Regression Equationsbull pH and Hardness

Accounting for Effects of Water Chemistry on Bioavailability and Toxicity

bull Water quality criteria can be modified quantitatively when enough data are available to demonstrate that those conditions similarly affect the results with a variety of speciesndash High quality data in the draft aluminum document demonstrated

that pH DOC and hardness affected a variety of species in a similar way when exposed to aluminum concentrations

bull Recent publications by Cardwell et al (2017) and Gensemer et al (2017) summarized short-term aluminum chronic toxicity data across a range of DOC pH and hardness values for three species

18

Multiple Linear Regression (MLR) Models to Account for Effects of Water Chemistry

bull MLR models were developed for each species (DeForest et al 2017)ndash 119862119862 119889119889119906119906119887119887119894119894119886119886 11986411986411986211986220=119890119890[minus41026+[0525timesln(119863119863119874119874119862119862)]+[2201timesln(ℎ119886119886119903119903119889119889)]+(11282times119901119901119867119867)minus(0633times1199011199011198671198672)minus[0264times119901119901119867119867ln(ℎ119886119886119903119903119889119889)]]

ndash 119875119875 119901119901119903119903119900119900119898119898119890119890119897119897119886119886119904119904 11986411986411986211986220=119890119890[minus14029+[0503timesln(119863119863119874119874119862119862)]+[3443timesln(ℎ119886119886119903119903119889119889)]+(3131times119901119901119867119867)minus[0494times119901119901119867119867ln(ℎ119886119886119903119903119889119889)]]

bull These models were applied to both the acute and chronic aluminum toxicity datandash The C dubia model was applied to invertebrate datandash The P promelas model was applied to vertebrate data

19

III Normalize toxicity data in species sensitivity distribution to a set of water

quality conditionsbull Just like single parameter hardness models the MLR

models were used to normalize the aluminum toxicity data to a single set of water quality conditionsndash As an illustration for one set of calculations the draft

aluminum AWQC set these conditions to pH 7 hardness of 100 mgL and DOC of 1 mgL

bull This allows for comparisons to be made between species based on their sensitivity to aluminumndash Apples to apples

20

IV Construct sensitivity distribution to determine criteria at set conditions

21

Figure 8 Ranked Summary of Total Aluminum Genus Mean Acute Values (GMAVs) - Freshwater at pH 7 Hardness of 100 mgL and DOC of 1 mgL

IV Construct sensitivity distribution to determine criteria at set conditions

22

Figure 10 Ranked Summary of Total Aluminum Genus Mean Chronic Values (GMCVs) ndash Freshwater Supplemented with Other Data to Fulfill Missing MDRs at pH 7 Hardness of 100 mgL and DOC of 1 mgL

Aluminum Criteria Calculatorbull The relative ranking of genera in the sensitivity

distribution to which the toxicity data are normalized will change based on the set of water chemistry conditions (ie pH hardness and DOC)

bull To make calculating aluminum criteria easier EPA developed an Aluminum Criteria Calculatorndash Automated so that user only needs to enter water chemistry

conditions of interestndash Allows quick calculations for a variety of conditionsndash Limits calculations to the recommended conditions for which

the MLR models were developed were specified for hardness and DOC

bull No extrapolation beyond hardness and DOC empirical data bounds

23

Aluminum Criteria Calculator SD Example

24

pH=7 Hardness=100 DOC=1 pH=6 Hardness=25 DOC=1

Chronic Ranked GMCVRank GMCV Genus

12 17098 Aeolosoma Invert11 gt 9746 Rana Amphib - Other Data10 5908 Chironomus Invert

9 4877 Lymnaea Mollusk8 2555 Brachionus Invert7 2488 Pimephales Fish6 1713 Hyalella Invert5 1182 Ceriodaphnia Invert4 1102 Danio Fish3 1042 Lampsilis Mollusk2 591 Salvelinus Fish1 509 Salmo Fish

FCV = 3940 microgL total aluminum

Chronic Ranked GMCVRank GMCV Genus

12 gt 2131 Rana Amphib - Other Data11 1710 Aeolosoma Invert10 591 Chironomus Invert

9 544 Pimephales Fish8 488 Lymnaea Mollusk7 255 Brachionus Invert6 241 Danio Fish5 171 Hyalella Invert4 129 Salvelinus Fish3 118 Ceriodaphnia Invert2 111 Salmo Fish1 104 Lampsilis Mollusk

FCV = 987 microgL total aluminum

Multiple Scenarios

Run Scenarios

Summary Sheet

Acute Dataset

Chronic Dataset

Multiple Scenarios

Run Scenarios

Summary Sheet

Acute Dataset

Chronic Dataset

Boundaries of the Criteria Outputsbull MLR models were developed using data that

encompassndash DOC range of 008-5 mgLndash Hardness range of 98-127 mgL (as CaCO3)ndash pH range of 6-81

bull MLR criteria outputs are bounded atndash Maximum of 150 mgL total hardness (as CaCO3)

ndash DOC of 50 mgLndash pH range of the model is 50 to 90

25

Aluminum Criteria Look-Up Tables

26

bull EPA also provided criteria look-up tables in Appendix K

Har

dnes

s

CCC(microgL total aluminum)

(DOC=05 mgL)

pH 50 pH 55 pH 60 pH 65 pH 70 pH 75 pH 80 pH 85 pH 90

25 23 a 14 b 69 c 160 f 350 f 620 i 540 b 410 a 230 a

50 43 a 24 b 100 d 170 f 310 f 630 f 670 j 400 a 200 a

75 61 a 33 b 130 e 180 f 290 f 530 f 730 i 390 a 190 a

100 78 a 41 b 150 e 190 f 280 g 460 f 770 c 390 a 180 a

150 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

200 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

250 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

300 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

350 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

400 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

Aluminum Criteria Look-Up Tables

27

bull EPA also provided criteria look-up tables in Appendix K

Har

dnes

s

CCC(microgL total aluminum)

(DOC=10 mgL)

pH 50 pH 55 pH 60 pH 65 pH 70 pH 75 pH 80 pH 85 pH 90

25 33 a 21 b 99 c 220 f 490 f 900 i 780 b 590 a 330 a

50 61 a 35 b 150 e 250 f 440 f 890 f 960 j 580 a 290 a

75 88 a 47 b 180 e 260 f 410 f 740 f 1100 i 570 a 270 a

100 11 a 59 b 210 e 270 f 390 g 650 f 1100 c 560 b 260 a

150 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

200 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

250 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

300 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

350 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

400 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

Aluminum Criteria Look-Up Tables

28

bull EPA also provided criteria look-up tables in Appendix K

Har

dnes

s

CCC(microgL total aluminum)

(DOC=25 mgL)

pH 50 pH 55 pH 60 pH 65 pH 70 pH 75 pH 80 pH 85 pH 90

25 54 a 34 b 160 c 350 f 770 f 1500 i 1300 b 960 a 540 a

50 99 a 57 b 230 e 390 f 690 f 1400 f 1600 j 930 a 470 a

75 14 a 77 b 290 e 410 f 640 f 1200 f 1700 i 920 a 440 a

100 18 a 96 b 330 e 420 f 620 g 1000 f 1800 d 920 b 420 a

150 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

200 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

250 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

300 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

350 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

400 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

Aluminum Criteria Look-Up Tables

29

bull EPA also provided criteria look-up tables in Appendix K

Har

dnes

s

CCC(microgL total aluminum)

(DOC=50 mgL)

pH 50 pH 55 pH 60 pH 65 pH 70 pH 75 pH 80 pH 85 pH 90

25 77 a 49 b 230 c 500 f 1100 f 2100 c 1900 b 1400 a 770 a

50 14 a 82 b 330 e 540 f 970 f 2000 f 2200 j 1300 a 680 a

75 20 a 110 b 410 e 570 f 900 f 1600 f 2500 i 1300 a 640 a

100 26 a 140 b 460 f 590 f 880 g 1400 f 2500 d 1300 b 600 a

150 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

200 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

250 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

300 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

350 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

400 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

Status

30

bull Draft Document Released to the Federal Register on 72817ndash Public comment period of 60 days ndash Due Date for Comments is 92617

bull Revisions will be made considering public commentsbull Subsequent EPA intra-agency review of final criteria

document will then be conducted bull Publication of final criteria document planned for Late Fall

2017

Federal Register Webpagehttpswwwfederalregistergovdocuments201

707282017-15968request-for-scientific-views-draft-updated-aquatic-life-ambient-water-quality-criteria-for-aluminum

Aluminum Criteria Webpagehttpswwwepagovwqc2017-draft-aquatic-life-

criteria-aluminum-freshwater-documents

31

	Appendix C Acceptable Chronic Toxicity Data of Aluminum to Freshwater Aquatic Animals
									MLR equation
	Species		Hardness (mgL as CaCO3)		pH		DOC(mgL)		EC20 Endpoint			EC20(microgL)			Normalized Conc- EC20(microgL)			SMCV-EC20(microgL)		GMCV-EC20(microgL)			Genus			DOC Notes		Dilution water
	Oligochaete Aeolosoma sp		48		6		025		Reproduction (population size)			1235			1710			1710		1710			Aeolosoma		Invert		Author reported half the detection limit
	Rotifer Brachionus calyciflorus		100		62		025		Reproduction (population size)			431			255			255		255			Brachionus		Invert		Author reported half the detection limit
	Great pond snail Lymnaea stagnalis		117		61		025		Biomass			746			488			488		488			Lymnaea		Mollusk		Author reported half the detection limit
	Fatmucket Lampsilis siliquoidea		1055		604		040		Biomass			169			104			104		104			Lampsilis		Mollusk		Author reported
	Cladoceran Ceriodaphnia dubia		50		715		11		Reproduction - youngstarting adult			1780			190						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		26		73		05		Reproduction - youngfemale			1557			272						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		46		75		05		Reproduction - youngfemale			8087			110						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		96		79		05		Reproduction - youngfemale			6519			80						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		194		81		05		Reproduction - youngfemale			6836			89						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		255		703		05		Reproduction (youngfemale)			250			56						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		122		713		05		Reproduction (youngfemale)			860			106						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		25		798		05		Reproduction (youngfemale)			700			101						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		61		803		05		Reproduction (youngfemale)			1010			136						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		122		810		05		Reproduction (youngfemale)			870			116						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		255		633		05		Reproduction (youngfemale)			260			176						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		121		636		05		Reproduction (youngfemale)			390			110			118		118			Ceriodaphnia		Invert		Author reported
	Amphipod Hyalella azteca		95		61		051		Biomass (28 d)			1993			101						Hyalella			Author reported
	Amphipod Hyalella azteca		106		604		033		Biomass (28 d)			425			289			171		171			Hyalella		Invert		Author reported
	Midge Chironomus riparius		118		558		18		Adult midge emergence			2955			119						Chironomus			Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Midge Chironomus riparius		119		505		18		Adult midge emergence			8442			2258						Chironomus			Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Midge Chironomus riparius		91		66		051		Reproduction ( of eggscase)			3387			766			591		591			Chironomus		Invert		Author reported
	Atlantic salmon Salmo salar		127		57		18		Biomass			6156			1112			1112		1112			Salmo		Fish		Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Brook trout Salvelinus fontinalis		123		655		19		Biomass			1644			59						Salvelinus			Author reported		wellRO mix
	Brook trout Salvelinus fontinalis		128		565		18		Biomass			1435			283			1293		1293			Salvelinus		Fish		Author reported		wellRO mix
	Fathead minnow Pimephales promelas		220		770		16		Biomass			6194			781						Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		well water
	Fathead minnow Pimephales promelas		96		620		025		Fry survival			4286			379			544		544			Pimephales		Fish		Author reported half the detection limit
	Zebrafish Danio rerio		83		61		025		Biomass			2344			241			241		241			Danio		Fish		Author reported half the detection limit
	Wood frog Rana sylvatica		115		469		16		MATC-survival		gt		2000		gt		2131		gt		2131		2131		gt		Rana		Amphib - Other Data		Value is from 2007 FW Cu AWQC Appendix C recommendation		aged tap water

	Appendix A Acceptable Acute Toxicity Data of Aluminum to Freshwater Aquatic Animals
	Species		Methoda		Hardness (mgL as CaCO3)		pH		DOC(mgL)			LC50 or EC50 Conc (microgL)		Reference			Normalized Conc - LC50(microgL)			Species Mean Acute Value(microgL)		Genus Mean Acute Value(microgL)			Genus			DOC Note		Dilution water
	Snail Physa sp		S M T		474		659		11		gt		23400		Call 1984 Call et al 1984		gt		4843			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		755		11			30600		Call 1984 Call et al 1984			2699			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		817		11		gt		24700		Call 1984 Call et al 1984		gt		2404			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		746		11			55500		Call 1984 Call et al 1984			5034			3546		3546			Physa		Mollusk		Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Fatmucket Lampsilis siliquoidea		F M T		1060		612		048		gt		6302		Wang et al 2016 Wang et al 2017		gt		2983		gt		2983		2983		gt		Lampsilis		Mollusk		Author reported
	Cladoceran Ceriodaphnia dubia		S M A		500		742		11			1900		McCauley et al 1986			173			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		S M A		505		786		11			1500		McCauley et al 1986			129			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		UW-S Lake Superior lab water
	Cladoceran Ceriodaphnia dubia		S M A		500		813		11			2560		McCauley et al 1986			243			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		R M T		26		75		05			720		ENSR 1992d			111			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		R M T		46		76		05			1880		ENSR 1992d			250			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		R M T		96		78		05			2450		ENSR 1992d			289			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		S U NR		90		715		05			3727		Fort and Stover 1995			500			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U NR		90		715		05			5673		Fort and Stover 1995			761			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U NR		89		76		05			2880		Soucek et al 2001			336			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		601		05			7112		European Al Association 2009			169			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		605		2			6865		European Al Association 2009			709			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		609		4			15581		European Al Association 2009			1007			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		601		05			681		European Al Association 2009			162			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		603		05			1630		European Al Association 2009			367			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		597		05			1785		European Al Association 2009			473			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		592		05			1410		European Al Association 2009			429			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		699		05		gt		1300		European Al Association 2009		gt		419			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		785		05		gt		5000		European Al Association 2009		gt		796			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		68		2		gt		10000		European Al Association 2009		gt		2076			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		782		2		gt		15000		European Al Association 2009		gt		1163			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		677		4		gt		10000		European Al Association 2009		gt		1516			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		766		4		gt		15000		European Al Association 2009		gt		861			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		79		05		gt		2000		European Al Association 2009		gt		315			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		789		05		gt		2000		European Al Association 2009		gt		316			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		604		05			1108		European Al Association 2009			85			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		598		2			11371		European Al Association 2009			482			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		573		4			80467		European Al Association 2009			4357			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		671		05		gt		10000		European Al Association 2009		gt		2379			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		783		05		gt		5000		European Al Association 2009		gt		634			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		679		2		gt		10000		European Al Association 2009		gt		1039			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		767		2		gt		15000		European Al Association 2009		gt		908			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		668		4		gt		15000		European Al Association 2009		gt		1246			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		762		4		gt		15000		European Al Association 2009		gt		633			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		606		2			33868		European Al Association 2009			792			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		56		4			104842		European Al Association 2009			4880			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		693		05		gt		5000		European Al Association 2009		gt		713			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		120		788		05		gt		5000		European Al Association 2009		gt		590			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		120		676		2		gt		15000		European Al Association 2009		gt		1212			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		771		2		gt		15000		European Al Association 2009		gt		810			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		66		4		gt		15000		European Al Association 2009		gt		1014			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		76		4		gt		15000		European Al Association 2009		gt		555			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			11971		European Al Association 2010			270			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			27478		European Al Association 2010			619			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			11998		European Al Association 2010			270			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		607		05			92495		European Al Association 2010			188			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		609		05			31337		European Al Association 2010			604			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		61		05			33235		European Al Association 2010			624			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		708		05		gt		8864		European Al Association 2010		gt		253			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		779		05		gt		42783		European Al Association 2010		gt		693			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		753		05			13204		European Al Association 2010			24			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		600		601		05			46326		European Al Association 2010			380			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		600		599		05		gt		8590		European Al Association 2010		gt		737			509				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia reticulata		F M T		451		60		11			304		Shephard 1983			201			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia reticulata		F M T		40		55		11			362		Shephard 1983			5531			1054		733			Ceriodaphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake SuperiorDI Mix
	Cladoceran Daphnia magna		S U NR		453		774		11			3900		Biesinger and Christensen 1972			338			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Daphnia magna		S M T		220		76		16			38200		Kimball 1978			2030			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Well water
	Cladoceran Daphnia magna		S U T		451		725		11			2800		Shephard 1983			287			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Daphnia magna		S U T		168		599		05			5000		European Al Association 2009		gt		227			-				Daphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Daphnia magna		S U T		168		792		05			7950		European Al Association 2009			92			333		333			Daphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Ostracod Stenocypris major		R M T		1563		651		16			310196		Shuhaimi-Othman et al 2011a			1022			1022		1022			Stenocypris		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (Malaysia)
	Amphipod Crangonyx pseudogracilis		R U T		50		675		16			9190		Martin and Holdich 1986			1217			1217		1217			Crangonyx		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (UK)
	Amphipod Hyalella azteca		F M T		105		613		048		gt		5997		Wang et al 2016 Wang et al 2017		gt		2800		gt		2800		2800		gt		Hyalella		Invert		Author reported
	Stonefly Acroneuria sp		S M T		474		746		11		gt		22600		Call et al 1984		gt		2050		gt		2050		2050		gt		Acroneuria		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Midge Chironomus plumosus		S U T		80		70		16			30000		Fargasova 2001			2536			2536		2536			Chironomus		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (Slovakia)
	Midge Paratanytarsus dissimilis		S M T		1743		728		28		gt		77700		Lamb and Bailey 1981 1983		gt		6232		gt		6232		6232		gt		Paratanytarsus		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Liberty Lake Washington
	Rainbow trout Oncorhynchus mykiss		F M T		274		758		05		gt		9840		Gundersen et al 1994		gt		1256			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		452		762		05		gt		8070		Gundersen et al 1994		gt		1140			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		895		758		05		gt		8160		Gundersen et al 1994		gt		1489			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		1304		758		05		gt		8200		Gundersen et al 1994		gt		1676			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		232		825		05			6170		Gundersen et al 1994			260			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		350		826		05			6170		Gundersen et al 1994			333			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		836		829		05			7670		Gundersen et al 1994			701			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		1158		830		05			6930		Gundersen et al 1994			777			801		801			Oncorhynchus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Atlantic salmon Salmo salar		S U T		68		55		05			584		Hamilton and Haines 1995			4602			-				Salmo			Value is from 2007 FW Cu AWQC Appendix C recommendation		Soft recon
	Atlantic salmon Salmo salar		S U T		68		65		05			599		Hamilton and Haines 1995			531			1564		1564			Salmo		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Soft recon
	Brook trout Salvelinus fontinalis		S U T		40		56		16			6530		Tandjung 1982			6947			-				Salvelinus			Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Brook trout Salvelinus fontinalis		S U T		18		56		16			3400		Tandjung 1982			6209			-				Salvelinus			Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Brook trout Salvelinus fontinalis		S U T		2		56		16			370		Tandjung 1982			2988			5051		5051			Salvelinus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Green sunfish Lepomis cyanellus		S M T		474		755		11		gt		50000		Call et al 1984		gt		5255		gt		5255		5255		gt		Lepomis		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Rio Grande silvery minnow Hybognathus amarus		R M T		140		81		05		gt		59100		Buhl 2002		gt		8620		gt		8620		8620		gt		Hybognathus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon water
	Fathead minnow Pimephales promelas		S M T		474		761		11		gt		48200		Call et al 1984		gt		4707			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Fathead minnow Pimephales promelas		S M T		474		805		11		gt		49800		Call et al 1984		gt		2837			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Fathead minnow Pimephales promelas		R M T		26		78		05			1160		ENSR 1992c			104			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		46		76		05			8180		ENSR 1992c			1191			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		96		81		05			20300		ENSR 1992c			2398			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		194		81		05			44800		ENSR 1992c			7839			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		140		81		05		gt		59100		Buhl 2002		gt		8620			2224		2224			Pimephales		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon water
	Smallmouth bass Micropterus dolomieui		S M T		1245		505		16			130		Kane and Rabeni 1987			859			859		859			Micropterus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Green tree frog Hyla cinerea		R M T		455		549		05		gt		4052		Jung and Jagoe 1995		gt		4377		gt		4377		4377		gt		Hyla		Amphibian		Value is from 2007 FW Cu AWQC Appendix C recommendation		artificial soft recon

	Normalization Chemistry
	pH		6					FAV		360
	Hardness		25					CMC		180
	DOC		1					CCC		99
	Acute Ranked GMAV											Chronic Ranked GMCV
	Rank			GMAV		Genus								Rank			GMCV		Genus
	18		gt		8620		Hybognathus		Fish							12		gt		2131		Rana		Amphib - Other Data
	17		gt		6232		Paratanytarsus		Invert							11			1710		Aeolosoma		Invert
	16		gt		5255		Lepomis		Fish							10			591		Chironomus		Invert
	15			5051		Salvelinus		Fish							9			544		Pimephales		Fish
	14		gt		4377		Hyla		Amphibian							8			488		Lymnaea		Mollusk
	13			3546		Physa		Mollusk							7			255		Brachionus		Invert
	12		gt		2983		Lampsilis		Mollusk							6			241		Danio		Fish
	11		gt		2800		Hyalella		Invert							5			171		Hyalella		Invert
	10			2536		Chironomus		Invert							4			129		Salvelinus		Fish
	9			2224		Pimephales		Fish							3			118		Ceriodaphnia		Invert
	8		gt		2050		Acroneuria		Invert							2			111		Salmo		Fish
	7			1564		Salmo		Fish							1			104		Lampsilis		Mollusk
	6			1217		Crangonyx		Invert
	5			1022		Stenocypris		Invert
	4			859		Micropterus		Fish
	3			801		Oncorhynchus		Fish
	2			733		Ceriodaphnia		Invert
	1			333		Daphnia		Invert
		N		Rank		Genus		GMAV		ln(GMAV)		ln(GMAV)2		P=R(N+1)		sqrt(P)				N		Rank		Genus		GMCV		ln(GMCV)		ln(GMCV)2		P=R(N+1)		sqrt(P)
		18		4		Micropterus		859		676		4564		0211		0459				12		4		Salvelinus		129		486		2364		0308		0555
			3		Oncorhynchus		801		669		4469		0158		0397					3		Ceriodaphnia		118		477		2277		0231		0480
			2		Ceriodaphnia		733		660		4352		0105		0324					2		Salmo		111		471		2220		0154		0392
			1		Daphnia		333		581		3374		0053		0229					1		Lampsilis		104		465		2159		0077		0277
			Sum				2585		1676		0526		141					Sum				1899		902		0769		170
			S2 =		1987										S2 =		059
			L =		4890										L =		4420
			A =		5887										A =		4592
			FAV =		360										FCV =		987
			CMC =		180

				All concentrations reported are microgL total Aluminum
				lt-----Click Button after entering data
	ENTER DATA HERE									ERRORNUM		ACUTE														CHRONIC
										Rank 1				Rank 2				Rank 3				Rank 4					Rank 1				Rank 2				Rank 3				Rank 4
	SiteName		DOC(mgL)		Hardness(mgL as CaCO3)		pH			FAV		CMC		CCC			Genus		GMAV			Genus		GMAV			Genus		GMAV			Genus		GMAV				Genus		GMCV			Genus		GMCV			Genus		GMCV			Genus		GMCV
	Site 1		50		25		9		75428737034162		35229324666008		1800		770			Daphnia			33452		Ceriodaphnia			73576		Stenocypris			102576		Crangonyx			122230			Lampsilis			10463		Ceriodaphnia			11865		Hyalella			17200		Brachionus			25648
	Site 2		50		50		9		75428737034162		31204991509063		1600		680			Daphnia			29631		Ceriodaphnia			65171		Stenocypris			90859		Crangonyx			108267			Lampsilis			9268		Ceriodaphnia			10510		Hyalella			15236		Brachionus			22718
	Site 3		50		75		9		75428737034162		29067527928199		1500		640			Daphnia			27601		Ceriodaphnia			60707		Stenocypris			84635		Crangonyx			100851			Lampsilis			8633		Ceriodaphnia			9790		Hyalella			14192		Brachionus			21162
	Site 4		50		100		9		75428737034162		27640367912592		1400		600			Daphnia			26246		Ceriodaphnia			57726		Stenocypris			80480		Crangonyx			95899			Lampsilis			8209		Ceriodaphnia			9309		Hyalella			13495		Brachionus			20123
	Site 5		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 6		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 7		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 8		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 9		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 10		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 11		50		25		85		75428737034162		63245275664384		3200		1400			Daphnia			60055		Ceriodaphnia			132086		Stenocypris			184149		Crangonyx			219432			Lampsilis			18783		Ceriodaphnia			21301		Hyalella			30879		Brachionus			46045
	Site 12		50		50		85		75428737034162		61388044609926		3100		1300			Daphnia			58291		Ceriodaphnia			128208		Stenocypris			178742		Crangonyx			212988			Lampsilis			18232		Ceriodaphnia			20676		Hyalella			29972		Brachionus			44693
	Site 13		50		75		85		75428737034162		60327020429132		3000		1300			Daphnia			57284		Ceriodaphnia			125992		Stenocypris			175652		Crangonyx			209307			Lampsilis			17917		Ceriodaphnia			20318		Hyalella			29454		Brachionus			43920
	Site 14		50		100		85		75428737034162		59585352130146		3000		1300			Daphnia			56580		Ceriodaphnia			124443		Stenocypris			173493		Crangonyx			206734			Lampsilis			17696		Ceriodaphnia			20068		Hyalella			29092		Salmo			41264
	Site 15		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 16		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 17		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 18		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 19		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 20		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370

	Appendix C Acceptable Chronic Toxicity Data of Aluminum to Freshwater Aquatic Animals
									MLR equation
	Species		Hardness (mgL as CaCO3)		pH		DOC(mgL)		EC20 Endpoint			EC20(microgL)			Normalized Conc- EC20(microgL)			SMCV-EC20(microgL)		GMCV-EC20(microgL)			Genus			DOC Notes		Dilution water
	Oligochaete Aeolosoma sp		48		6		025		Reproduction (population size)			1235			17098			17098		17098			Aeolosoma		Invert		Author reported half the detection limit
	Rotifer Brachionus calyciflorus		100		62		025		Reproduction (population size)			431			2555			2555		2555			Brachionus		Invert		Author reported half the detection limit
	Great pond snail Lymnaea stagnalis		117		61		025		Biomass			746			4877			4877		4877			Lymnaea		Mollusk		Author reported half the detection limit
	Fatmucket Lampsilis siliquoidea		1055		604		040		Biomass			169			1042			1042		1042			Lampsilis		Mollusk		Author reported
	Cladoceran Ceriodaphnia dubia		50		715		11		Reproduction - youngstarting adult			1780			1898						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		26		73		05		Reproduction - youngfemale			1557			2718						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		46		75		05		Reproduction - youngfemale			8087			1102						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		96		79		05		Reproduction - youngfemale			6519			796						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		194		81		05		Reproduction - youngfemale			6836			888						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		255		703		05		Reproduction (youngfemale)			250			563						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		122		713		05		Reproduction (youngfemale)			860			1061						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		25		798		05		Reproduction (youngfemale)			700			1006						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		61		803		05		Reproduction (youngfemale)			1010			1363						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		122		810		05		Reproduction (youngfemale)			870			1164						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		255		633		05		Reproduction (youngfemale)			260			1758						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		121		636		05		Reproduction (youngfemale)			390			1101			1182		1182			Ceriodaphnia		Invert		Author reported
	Amphipod Hyalella azteca		95		61		051		Biomass (28 d)			1993			1014						Hyalella			Author reported
	Amphipod Hyalella azteca		106		604		033		Biomass (28 d)			425			2895			1713		1713			Hyalella		Invert		Author reported
	Midge Chironomus riparius		118		558		18		Adult midge emergence			2955			1192						Chironomus			Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Midge Chironomus riparius		119		505		18		Adult midge emergence			8442			22578						Chironomus			Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Midge Chironomus riparius		91		66		051		Reproduction ( of eggscase)			3387			7664			5908		5908			Chironomus		Invert		Author reported
	Atlantic salmon Salmo salar		127		57		18		Biomass			6156			5085			5085		5085			Salmo		Fish		Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Brook trout Salvelinus fontinalis		123		655		19		Biomass			1644			270						Salvelinus			Author reported		wellRO mix
	Brook trout Salvelinus fontinalis		128		565		18		Biomass			1435			1295			5914		5914			Salvelinus		Fish		Author reported		wellRO mix
	Fathead minnow Pimephales promelas		220		770		16		Biomass			6194			3569						Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		well water
	Fathead minnow Pimephales promelas		96		620		025		Fry survival			4286			1734			2488		2488			Pimephales		Fish		Author reported half the detection limit
	Zebrafish Danio rerio		83		61		025		Biomass			2344			1102			1102		1102			Danio		Fish		Author reported half the detection limit
	Wood frog Rana sylvatica		115		469		16		MATC-survival		gt		2000		gt		9746		gt		9746		9746		gt		Rana		Amphib - Other Data		Value is from 2007 FW Cu AWQC Appendix C recommendation		aged tap water

	Appendix A Acceptable Acute Toxicity Data of Aluminum to Freshwater Aquatic Animals
	Species		Methoda		Hardness (mgL as CaCO3)		pH		DOC(mgL)			LC50 or EC50 Conc (microgL)		Reference			Normalized Conc - LC50(microgL)			Species Mean Acute Value(microgL)		Genus Mean Acute Value(microgL)			Genus			DOC Note		Dilution water
	Snail Physa sp		S M T		474		659		11		gt		23400		Call 1984 Call et al 1984		gt		48425			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		755		11			30600		Call 1984 Call et al 1984			26986			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		817		11		gt		24700		Call 1984 Call et al 1984		gt		24040			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		746		11			55500		Call 1984 Call et al 1984			50338			35462		35462			Physa		Mollusk		Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Fatmucket Lampsilis siliquoidea		F M T		1060		612		048		gt		6302		Wang et al 2016 Wang et al 2017		gt		29834		gt		29834		29834		gt		Lampsilis		Mollusk		Author reported
	Cladoceran Ceriodaphnia dubia		S M A		500		742		11			1900		McCauley et al 1986			1728			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		S M A		505		786		11			1500		McCauley et al 1986			1294			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		UW-S Lake Superior lab water
	Cladoceran Ceriodaphnia dubia		S M A		500		813		11			2560		McCauley et al 1986			2431			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		R M T		26		75		05			720		ENSR 1992d			1113			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		R M T		46		76		05			1880		ENSR 1992d			2495			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		R M T		96		78		05			2450		ENSR 1992d			2894			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		S U NR		90		715		05			3727		Fort and Stover 1995			5002			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U NR		90		715		05			5673		Fort and Stover 1995			7613			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U NR		89		76		05			2880		Soucek et al 2001			3362			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		601		05			7112		European Al Association 2009			1691			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		605		2			6865		European Al Association 2009			7088			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		609		4			15581		European Al Association 2009			10072			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		601		05			681		European Al Association 2009			1620			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		603		05			1630		European Al Association 2009			3674			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		597		05			1785		European Al Association 2009			4732			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		592		05			1410		European Al Association 2009			4295			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		699		05		gt		1300		European Al Association 2009		gt		4189			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		785		05		gt		5000		European Al Association 2009		gt		7960			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		68		2		gt		10000		European Al Association 2009		gt		20755			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		782		2		gt		15000		European Al Association 2009		gt		11626			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		677		4		gt		10000		European Al Association 2009		gt		15161			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		766		4		gt		15000		European Al Association 2009		gt		8608			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		79		05		gt		2000		European Al Association 2009		gt		3150			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		789		05		gt		2000		European Al Association 2009		gt		3156			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		604		05			1108		European Al Association 2009			850			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		598		2			11371		European Al Association 2009			4817			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		573		4			80467		European Al Association 2009			43570			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		671		05		gt		10000		European Al Association 2009		gt		23786			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		783		05		gt		5000		European Al Association 2009		gt		6344			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		679		2		gt		10000		European Al Association 2009		gt		10394			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		767		2		gt		15000		European Al Association 2009		gt		9082			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		668		4		gt		15000		European Al Association 2009		gt		12461			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		762		4		gt		15000		European Al Association 2009		gt		6332			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		606		2			33868		European Al Association 2009			7920			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		56		4			104842		European Al Association 2009			48800			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		693		05		gt		5000		European Al Association 2009		gt		7126			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		120		788		05		gt		5000		European Al Association 2009		gt		5898			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		120		676		2		gt		15000		European Al Association 2009		gt		12117			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		771		2		gt		15000		European Al Association 2009		gt		8096			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		66		4		gt		15000		European Al Association 2009		gt		10139			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		76		4		gt		15000		European Al Association 2009		gt		5545			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			11971		European Al Association 2010			2699			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			27478		European Al Association 2010			6194			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			11998		European Al Association 2010			2705			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		607		05			92495		European Al Association 2010			1876			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		609		05			31337		European Al Association 2010			6035			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		61		05			33235		European Al Association 2010			6238			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		708		05		gt		8864		European Al Association 2010		gt		2534			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		779		05		gt		42783		European Al Association 2010		gt		6930			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		753		05			13204		European Al Association 2010			244			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		600		601		05			46326		European Al Association 2010			3798			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		600		599		05		gt		8590		European Al Association 2010		gt		7366			5094				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia reticulata		F M T		451		60		11			304		Shephard 1983			2009			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia reticulata		F M T		40		55		11			362		Shephard 1983			55307			10542		7328			Ceriodaphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake SuperiorDI Mix
	Cladoceran Daphnia magna		S U NR		453		774		11			3900		Biesinger and Christensen 1972			3382			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Daphnia magna		S M T		220		76		16			38200		Kimball 1978			20303			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Well water
	Cladoceran Daphnia magna		S U T		451		725		11			2800		Shephard 1983			2868			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Daphnia magna		S U T		168		599		05			5000		European Al Association 2009		gt		2265			-				Daphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Daphnia magna		S U T		168		792		05			7950		European Al Association 2009			920			3332		3332			Daphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Ostracod Stenocypris major		R M T		1563		651		16			310196		Shuhaimi-Othman et al 2011a			10216			10216		10216			Stenocypris		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (Malaysia)
	Amphipod Crangonyx pseudogracilis		R U T		50		675		16			9190		Martin and Holdich 1986			12174			12174		12174			Crangonyx		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (UK)
	Amphipod Hyalella azteca		F M T		105		613		048		gt		5997		Wang et al 2016 Wang et al 2017		gt		28002		gt		28002		28002		gt		Hyalella		Invert		Author reported
	Stonefly Acroneuria sp		S M T		474		746		11		gt		22600		Call et al 1984		gt		20498		gt		20498		20498		gt		Acroneuria		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Midge Chironomus plumosus		S U T		80		70		16			30000		Fargasova 2001			25361			25361		25361			Chironomus		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (Slovakia)
	Midge Paratanytarsus dissimilis		S M T		1743		728		28		gt		77700		Lamb and Bailey 1981 1983		gt		62318		gt		62318		62318		gt		Paratanytarsus		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Liberty Lake Washington
	Rainbow trout Oncorhynchus mykiss		F M T		274		758		05		gt		9840		Gundersen et al 1994		gt		5745			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		452		762		05		gt		8070		Gundersen et al 1994		gt		5212			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		895		758		05		gt		8160		Gundersen et al 1994		gt		6807			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		1304		758		05		gt		8200		Gundersen et al 1994		gt		7662			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		232		825		05			6170		Gundersen et al 1994			1190			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		350		826		05			6170		Gundersen et al 1994			1523			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		836		829		05			7670		Gundersen et al 1994			3205			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		1158		830		05			6930		Gundersen et al 1994			3554			3661		3661			Oncorhynchus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Atlantic salmon Salmo salar		S U T		68		55		05			584		Hamilton and Haines 1995			21042			-				Salmo			Value is from 2007 FW Cu AWQC Appendix C recommendation		Soft recon
	Atlantic salmon Salmo salar		S U T		68		65		05			599		Hamilton and Haines 1995			2430			7151		7151			Salmo		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Soft recon
	Brook trout Salvelinus fontinalis		S U T		40		56		16			6530		Tandjung 1982			31767			-				Salvelinus			Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Brook trout Salvelinus fontinalis		S U T		18		56		16			3400		Tandjung 1982			28391			-				Salvelinus			Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Brook trout Salvelinus fontinalis		S U T		2		56		16			370		Tandjung 1982			13663			23097		23097			Salvelinus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Green sunfish Lepomis cyanellus		S M T		474		755		11		gt		50000		Call et al 1984		gt		24028		gt		24028		24028		gt		Lepomis		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Rio Grande silvery minnow Hybognathus amarus		R M T		140		81		05		gt		59100		Buhl 2002		gt		39414		gt		39414		39414		gt		Hybognathus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon water
	Fathead minnow Pimephales promelas		S M T		474		761		11		gt		48200		Call et al 1984		gt		21522			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Fathead minnow Pimephales promelas		S M T		474		805		11		gt		49800		Call et al 1984		gt		12972			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Fathead minnow Pimephales promelas		R M T		26		78		05			1160		ENSR 1992c			477			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		46		76		05			8180		ENSR 1992c			5446			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		96		81		05			20300		ENSR 1992c			10966			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		194		81		05			44800		ENSR 1992c			35847			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		140		81		05		gt		59100		Buhl 2002		gt		39414			10168		10168			Pimephales		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon water
	Smallmouth bass Micropterus dolomieui		S M T		1245		505		16			130		Kane and Rabeni 1987			3929			3929		3929			Micropterus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Green tree frog Hyla cinerea		R M T		455		549		05		gt		4052		Jung and Jagoe 1995		gt		20016		gt		20016		20016		gt		Hyla		Amphibian		Value is from 2007 FW Cu AWQC Appendix C recommendation		artificial soft recon

	Normalization Chemistry
	pH		7					FAV		2741
	Hardness		100					CMC		1400
	DOC		1					CCC		390
	Acute Ranked GMAV											Chronic Ranked GMCV
	Rank			GMAV		Genus								Rank			GMCV		Genus
	18		gt		62318		Paratanytarsus		Invert							12			17098		Aeolosoma		Invert
	17		gt		39414		Hybognathus		Fish							11		gt		9746		Rana		Amphib - Other Data
	16			35462		Physa		Mollusk							10			5908		Chironomus		Invert
	15		gt		29834		Lampsilis		Mollusk							9			4877		Lymnaea		Mollusk
	14		gt		28002		Hyalella		Invert							8			2555		Brachionus		Invert
	13			25361		Chironomus		Invert							7			2488		Pimephales		Fish
	12		gt		24028		Lepomis		Fish							6			1713		Hyalella		Invert
	11			23097		Salvelinus		Fish							5			1182		Ceriodaphnia		Invert
	10		gt		20498		Acroneuria		Invert							4			1102		Danio		Fish
	9		gt		20016		Hyla		Amphibian							3			1042		Lampsilis		Mollusk
	8			12174		Crangonyx		Invert							2			591		Salvelinus		Fish
	7			10216		Stenocypris		Invert							1			509		Salmo		Fish
	6			10168		Pimephales		Fish
	5			7328		Ceriodaphnia		Invert
	4			7151		Salmo		Fish
	3			3929		Micropterus		Fish
	2			3661		Oncorhynchus		Fish
	1			3332		Daphnia		Invert
		N		Rank		Genus		GMAV		ln(GMAV)		ln(GMAV)2		P=R(N+1)		sqrt(P)				N		Rank		Genus		GMCV		ln(GMCV)		ln(GMCV)2		P=R(N+1)		sqrt(P)
		18		4		Salmo		7151		887		7876		0211		0459				12		4		Danio		1102		700		4907		0308		0555
			3		Micropterus		3929		828		6849		0158		0397					3		Lampsilis		1042		695		4829		0231		0480
			2		Oncorhynchus		3661		821		6733		0105		0324					2		Salvelinus		591		638		4074		0154		0392
			1		Daphnia		3332		811		6579		0053		0229					1		Salmo		509		623		3883		0077		0277
			Sum				3347		2804		0526		141					Sum				2657		1769		0769		170
			S2 =		1223										S2 =		1080
			L =		7134										L =		5242
			A =		7916										A =		5976
			FAV =		2741										FCV =		3940
			CMC =		1371

				All concentrations reported are microgL total Aluminum
				lt-----Click Button after entering data
	ENTER DATA HERE									ERRORNUM		ACUTE														CHRONIC
										Rank 1				Rank 2				Rank 3				Rank 4					Rank 1				Rank 2				Rank 3				Rank 4
	SiteName		DOC(mgL)		Hardness(mgL as CaCO3)		pH			FAV		CMC		CCC			Genus		GMAV			Genus		GMAV			Genus		GMAV			Genus		GMAV				Genus		GMCV			Genus		GMCV			Genus		GMCV			Genus		GMCV
	Site 1		50		25		9		75428737034162		35229324666008		1800		770			Daphnia			33452		Ceriodaphnia			73576		Stenocypris			102576		Crangonyx			122230			Lampsilis			10463		Ceriodaphnia			11865		Hyalella			17200		Brachionus			25648
	Site 2		50		50		9		75428737034162		31204991509063		1600		680			Daphnia			29631		Ceriodaphnia			65171		Stenocypris			90859		Crangonyx			108267			Lampsilis			9268		Ceriodaphnia			10510		Hyalella			15236		Brachionus			22718
	Site 3		50		75		9		75428737034162		29067527928199		1500		640			Daphnia			27601		Ceriodaphnia			60707		Stenocypris			84635		Crangonyx			100851			Lampsilis			8633		Ceriodaphnia			9790		Hyalella			14192		Brachionus			21162
	Site 4		50		100		9		75428737034162		27640367912592		1400		600			Daphnia			26246		Ceriodaphnia			57726		Stenocypris			80480		Crangonyx			95899			Lampsilis			8209		Ceriodaphnia			9309		Hyalella			13495		Brachionus			20123
	Site 5		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 6		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 7		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 8		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 9		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 10		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 11		50		25		85		75428737034162		63245275664384		3200		1400			Daphnia			60055		Ceriodaphnia			132086		Stenocypris			184149		Crangonyx			219432			Lampsilis			18783		Ceriodaphnia			21301		Hyalella			30879		Brachionus			46045
	Site 12		50		50		85		75428737034162		61388044609926		3100		1300			Daphnia			58291		Ceriodaphnia			128208		Stenocypris			178742		Crangonyx			212988			Lampsilis			18232		Ceriodaphnia			20676		Hyalella			29972		Brachionus			44693
	Site 13		50		75		85		75428737034162		60327020429132		3000		1300			Daphnia			57284		Ceriodaphnia			125992		Stenocypris			175652		Crangonyx			209307			Lampsilis			17917		Ceriodaphnia			20318		Hyalella			29454		Brachionus			43920
	Site 14		50		100		85		75428737034162		59585352130146		3000		1300			Daphnia			56580		Ceriodaphnia			124443		Stenocypris			173493		Crangonyx			206734			Lampsilis			17696		Ceriodaphnia			20068		Hyalella			29092		Salmo			41264
	Site 15		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 16		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 17		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 18		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 19		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 20		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370

Current 1988 Aluminum Criteriabull The current 1988 criteria account for the

ndash Influence of pH on toxicity (solely by limiting criteria application range)

ndash Similarity of acute toxicity of fish and invertebrates

ndash The greater sensitivity of invertebrates in chronic toxicity tests

5

Current 1988 Aluminum Criteriabull The final 1988 criteria document recommended

ndash An acute criterion of 750 microgL total aluminum when the pH is between 65 and 90

ndash Chronic criterion of 87 microgL total aluminum when the pH is between 65 and 90

ndash The 1988 freshwater chronic criterion of 87 microgL was lowered based on two tests (brook trout and striped bass)

ndash The studies were not pH adjusted and all the data were not normalized for hardness The 1988 existing criteria did not take hardness and DOC into account

6

Aluminum Standardsbull Aluminum Standards

ndash About 20 states have numeric criteria for aluminum for aquatic life

bull Most are based on EPArsquos 1988 values and two are hardness-based equations (CO and NM)

bull There are numerous site-specific criteria that involve the Biotic Ligand Model (BLM) or a Water Effects Ratio (WER) -adjusted 1988 aluminum criteria

ndash Aluminum is not a priority pollutant

7

Criteria Development Processbull All EPA Aquatic Life Criteria for Toxics undergo rigorous

scientific development and review processbull Develop criteria document draftbull Intra-agency peer review process bull Independent external peer reviewbull Revisions based on external peer review

Intra-agency review of draft criteria documentbull Release to the public in draft form to obtain scientific views

Revisions considering public comments Intra-agency review of final criteria document

bull Publication of final criteria document bull States may then consider recommended criteria in

development and adoption of state water quality standards8

Framework for Updated Draft Aluminum Criteria

I Screen toxicological studies for high

quality data

II Consider water conditions effects on

aluminum bioavailability

III Normalize toxicity data in species

sensitivity distribution to a set of water quality conditions

IV Construct sensitivity distribution to determine criteria

at set conditions

9

I Screen toxicological studies for high quality data

bull First step in all criteria developmentndash Data are evaluated based on the data quality objectives

outlined in the 1985 Guidelines methodology and other toxicity testing recommendations (ie EPA ASTM)

ndash High quality data are used quantitatively to develop criteria magnitudes

ndash Lower quality data are used qualitatively if appropriatendash Lowest quality data are considered unacceptable and not

usedbull Data are grouped into acute and chronic exposures

10

Aluminum Toxicity Databull The updated freshwater aluminum criteria meets the

acute and chronic minimum data requirements outlined in EPArsquos 1985 Guidelines

InvertebrateSpecies

Fish Species

AmphibianSpecies

Total Genera

Acute1988 AWQC 8 7 - 142017 Draft AWQC 11 8 1 18

Chronic1988 AWQC 2 1 - 32017 Draft AWQC 7 4 - 11

11 1988 AWQC Chronic CCC based on two studies for the striped

bass and brook trout

Aluminum Toxicity Data Freshwater mussels have been

suggested to be potentially sensitive to aluminum

EPA worked collaboratively with USGSrsquos Columbia Environmental Research Center (CERC) to have acute and chronic aluminum toxicity tests conducted with a unionid mussel (fatmucket Lampsilis siliquoidea) in water-only exposures to obtain additional data on invertebrates New data on amphipod (Hyalella azteca)

were collected to confirm laboratory conditions

12

Aluminum Toxicity Data

13

bull New data and models examinedndash Data submitted to EU REACH program

bull New freshwater aluminum toxicity data generated by Oregon State University

bull 2016 BLM model provided by the European Aluminum Association

ndash Saltwater data provided by Australia bull Insufficient data to develop estuarinemarine acute or chronic

criteria at this timendash Multiple Linear Regression (MLR) model developed by

DeForest et al 2017bull DeForest DK KV Brix LM Tear and WJ Adams 2017 Multiple Linear Regression (MLR) models for predicting chronic aluminum

toxicity to freshwater aquatic organisms and developing water quality guidelines Environ Toxicol Chem (DOI101002etc3922)

14

Minimum Dataset For Freshwater Criteria Derivation

SALMONID SECONDFISHFAMILY

CHORDATA

PLANKTONICCRUSTACEAN

BENTHIC CRUSTACEAN

INSECT ROTIFERA ANNELIDA MOLLUSCA

OTHERINSECT ORMOLLUSCA

EstuarineMarine Minimum Data Requirements

15

Family Minimum Data Requirement EstuarineMarine

Acute(Phylum Family Genus)

Chronic(Phylum Family Genus)

Family in the phylum Chordata - -

Family in the phylum Chordata - -

Either the Mysidae or Penaeidae family - -

Family in a phylum other than Arthropoda or Chordata Mollusca Ostreidae Crassostrea -

Family in a phylum other than Chordata Annelida Nereididae Neanthes -

Family in a phylum other than Chordata Annelida Capitellidae Capitella -

Family in a phylum other than Chordata Annelida Ctenodrilidae Ctenodrilus -

Any other family Arthropoda Ameiridae Nitokra -

II Evaluate water quality conditions to determine applicability

bull Criteria are often based on one or more water quality conditions (eg hardness) that affect toxicity of the material to test organisms

bull Aluminum toxicity is highly dependent on the pH of the test waterndash The pH of test waters affects the amount and form

of aluminum that is bioavailable to test speciesndash Hardness and DOC (Dissolved Organic Carbon)

also influence the amount of aluminum that is bioavailable

16

Multiple Bioavailability Modeling Approaches Underwent External Peer Review

17

bull Biotic Ligand Modelsbull Full aluminum BLM (2016 model version) ndash Temperature pH

Dissolved organic carbon (DOC) Major cations (calcium [Ca] magnesium [Mg] sodium [Na] and potassium [K]) Major anions (sulfate [SO4] and chloride [Cl]) Alkalinity and Sulfide

bull Simplified aluminum BLM - pH hardness DOC temperature

bull Multilinear Regression Equationsbull pH and Hardness

Accounting for Effects of Water Chemistry on Bioavailability and Toxicity

bull Water quality criteria can be modified quantitatively when enough data are available to demonstrate that those conditions similarly affect the results with a variety of speciesndash High quality data in the draft aluminum document demonstrated

that pH DOC and hardness affected a variety of species in a similar way when exposed to aluminum concentrations

bull Recent publications by Cardwell et al (2017) and Gensemer et al (2017) summarized short-term aluminum chronic toxicity data across a range of DOC pH and hardness values for three species

18

Multiple Linear Regression (MLR) Models to Account for Effects of Water Chemistry

bull MLR models were developed for each species (DeForest et al 2017)ndash 119862119862 119889119889119906119906119887119887119894119894119886119886 11986411986411986211986220=119890119890[minus41026+[0525timesln(119863119863119874119874119862119862)]+[2201timesln(ℎ119886119886119903119903119889119889)]+(11282times119901119901119867119867)minus(0633times1199011199011198671198672)minus[0264times119901119901119867119867ln(ℎ119886119886119903119903119889119889)]]

ndash 119875119875 119901119901119903119903119900119900119898119898119890119890119897119897119886119886119904119904 11986411986411986211986220=119890119890[minus14029+[0503timesln(119863119863119874119874119862119862)]+[3443timesln(ℎ119886119886119903119903119889119889)]+(3131times119901119901119867119867)minus[0494times119901119901119867119867ln(ℎ119886119886119903119903119889119889)]]

bull These models were applied to both the acute and chronic aluminum toxicity datandash The C dubia model was applied to invertebrate datandash The P promelas model was applied to vertebrate data

19

III Normalize toxicity data in species sensitivity distribution to a set of water

quality conditionsbull Just like single parameter hardness models the MLR

models were used to normalize the aluminum toxicity data to a single set of water quality conditionsndash As an illustration for one set of calculations the draft

aluminum AWQC set these conditions to pH 7 hardness of 100 mgL and DOC of 1 mgL

bull This allows for comparisons to be made between species based on their sensitivity to aluminumndash Apples to apples

20

IV Construct sensitivity distribution to determine criteria at set conditions

21

Figure 8 Ranked Summary of Total Aluminum Genus Mean Acute Values (GMAVs) - Freshwater at pH 7 Hardness of 100 mgL and DOC of 1 mgL

IV Construct sensitivity distribution to determine criteria at set conditions

22

Figure 10 Ranked Summary of Total Aluminum Genus Mean Chronic Values (GMCVs) ndash Freshwater Supplemented with Other Data to Fulfill Missing MDRs at pH 7 Hardness of 100 mgL and DOC of 1 mgL

Aluminum Criteria Calculatorbull The relative ranking of genera in the sensitivity

distribution to which the toxicity data are normalized will change based on the set of water chemistry conditions (ie pH hardness and DOC)

bull To make calculating aluminum criteria easier EPA developed an Aluminum Criteria Calculatorndash Automated so that user only needs to enter water chemistry

conditions of interestndash Allows quick calculations for a variety of conditionsndash Limits calculations to the recommended conditions for which

the MLR models were developed were specified for hardness and DOC

bull No extrapolation beyond hardness and DOC empirical data bounds

23

Aluminum Criteria Calculator SD Example

24

pH=7 Hardness=100 DOC=1 pH=6 Hardness=25 DOC=1

Chronic Ranked GMCVRank GMCV Genus

12 17098 Aeolosoma Invert11 gt 9746 Rana Amphib - Other Data10 5908 Chironomus Invert

9 4877 Lymnaea Mollusk8 2555 Brachionus Invert7 2488 Pimephales Fish6 1713 Hyalella Invert5 1182 Ceriodaphnia Invert4 1102 Danio Fish3 1042 Lampsilis Mollusk2 591 Salvelinus Fish1 509 Salmo Fish

FCV = 3940 microgL total aluminum

Chronic Ranked GMCVRank GMCV Genus

12 gt 2131 Rana Amphib - Other Data11 1710 Aeolosoma Invert10 591 Chironomus Invert

9 544 Pimephales Fish8 488 Lymnaea Mollusk7 255 Brachionus Invert6 241 Danio Fish5 171 Hyalella Invert4 129 Salvelinus Fish3 118 Ceriodaphnia Invert2 111 Salmo Fish1 104 Lampsilis Mollusk

FCV = 987 microgL total aluminum

Multiple Scenarios

Run Scenarios

Summary Sheet

Acute Dataset

Chronic Dataset

Multiple Scenarios

Run Scenarios

Summary Sheet

Acute Dataset

Chronic Dataset

Boundaries of the Criteria Outputsbull MLR models were developed using data that

encompassndash DOC range of 008-5 mgLndash Hardness range of 98-127 mgL (as CaCO3)ndash pH range of 6-81

bull MLR criteria outputs are bounded atndash Maximum of 150 mgL total hardness (as CaCO3)

ndash DOC of 50 mgLndash pH range of the model is 50 to 90

25

Aluminum Criteria Look-Up Tables

26

bull EPA also provided criteria look-up tables in Appendix K

Har

dnes

s

CCC(microgL total aluminum)

(DOC=05 mgL)

pH 50 pH 55 pH 60 pH 65 pH 70 pH 75 pH 80 pH 85 pH 90

25 23 a 14 b 69 c 160 f 350 f 620 i 540 b 410 a 230 a

50 43 a 24 b 100 d 170 f 310 f 630 f 670 j 400 a 200 a

75 61 a 33 b 130 e 180 f 290 f 530 f 730 i 390 a 190 a

100 78 a 41 b 150 e 190 f 280 g 460 f 770 c 390 a 180 a

150 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

200 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

250 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

300 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

350 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

400 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

Aluminum Criteria Look-Up Tables

27

bull EPA also provided criteria look-up tables in Appendix K

Har

dnes

s

CCC(microgL total aluminum)

(DOC=10 mgL)

pH 50 pH 55 pH 60 pH 65 pH 70 pH 75 pH 80 pH 85 pH 90

25 33 a 21 b 99 c 220 f 490 f 900 i 780 b 590 a 330 a

50 61 a 35 b 150 e 250 f 440 f 890 f 960 j 580 a 290 a

75 88 a 47 b 180 e 260 f 410 f 740 f 1100 i 570 a 270 a

100 11 a 59 b 210 e 270 f 390 g 650 f 1100 c 560 b 260 a

150 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

200 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

250 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

300 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

350 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

400 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

Aluminum Criteria Look-Up Tables

28

bull EPA also provided criteria look-up tables in Appendix K

Har

dnes

s

CCC(microgL total aluminum)

(DOC=25 mgL)

pH 50 pH 55 pH 60 pH 65 pH 70 pH 75 pH 80 pH 85 pH 90

25 54 a 34 b 160 c 350 f 770 f 1500 i 1300 b 960 a 540 a

50 99 a 57 b 230 e 390 f 690 f 1400 f 1600 j 930 a 470 a

75 14 a 77 b 290 e 410 f 640 f 1200 f 1700 i 920 a 440 a

100 18 a 96 b 330 e 420 f 620 g 1000 f 1800 d 920 b 420 a

150 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

200 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

250 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

300 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

350 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

400 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

Aluminum Criteria Look-Up Tables

29

bull EPA also provided criteria look-up tables in Appendix K

Har

dnes

s

CCC(microgL total aluminum)

(DOC=50 mgL)

pH 50 pH 55 pH 60 pH 65 pH 70 pH 75 pH 80 pH 85 pH 90

25 77 a 49 b 230 c 500 f 1100 f 2100 c 1900 b 1400 a 770 a

50 14 a 82 b 330 e 540 f 970 f 2000 f 2200 j 1300 a 680 a

75 20 a 110 b 410 e 570 f 900 f 1600 f 2500 i 1300 a 640 a

100 26 a 140 b 460 f 590 f 880 g 1400 f 2500 d 1300 b 600 a

150 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

200 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

250 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

300 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

350 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

400 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

Status

30

bull Draft Document Released to the Federal Register on 72817ndash Public comment period of 60 days ndash Due Date for Comments is 92617

bull Revisions will be made considering public commentsbull Subsequent EPA intra-agency review of final criteria

document will then be conducted bull Publication of final criteria document planned for Late Fall

2017

Federal Register Webpagehttpswwwfederalregistergovdocuments201

707282017-15968request-for-scientific-views-draft-updated-aquatic-life-ambient-water-quality-criteria-for-aluminum

Aluminum Criteria Webpagehttpswwwepagovwqc2017-draft-aquatic-life-

criteria-aluminum-freshwater-documents

31

	Appendix C Acceptable Chronic Toxicity Data of Aluminum to Freshwater Aquatic Animals
									MLR equation
	Species		Hardness (mgL as CaCO3)		pH		DOC(mgL)		EC20 Endpoint			EC20(microgL)			Normalized Conc- EC20(microgL)			SMCV-EC20(microgL)		GMCV-EC20(microgL)			Genus			DOC Notes		Dilution water
	Oligochaete Aeolosoma sp		48		6		025		Reproduction (population size)			1235			1710			1710		1710			Aeolosoma		Invert		Author reported half the detection limit
	Rotifer Brachionus calyciflorus		100		62		025		Reproduction (population size)			431			255			255		255			Brachionus		Invert		Author reported half the detection limit
	Great pond snail Lymnaea stagnalis		117		61		025		Biomass			746			488			488		488			Lymnaea		Mollusk		Author reported half the detection limit
	Fatmucket Lampsilis siliquoidea		1055		604		040		Biomass			169			104			104		104			Lampsilis		Mollusk		Author reported
	Cladoceran Ceriodaphnia dubia		50		715		11		Reproduction - youngstarting adult			1780			190						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		26		73		05		Reproduction - youngfemale			1557			272						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		46		75		05		Reproduction - youngfemale			8087			110						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		96		79		05		Reproduction - youngfemale			6519			80						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		194		81		05		Reproduction - youngfemale			6836			89						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		255		703		05		Reproduction (youngfemale)			250			56						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		122		713		05		Reproduction (youngfemale)			860			106						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		25		798		05		Reproduction (youngfemale)			700			101						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		61		803		05		Reproduction (youngfemale)			1010			136						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		122		810		05		Reproduction (youngfemale)			870			116						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		255		633		05		Reproduction (youngfemale)			260			176						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		121		636		05		Reproduction (youngfemale)			390			110			118		118			Ceriodaphnia		Invert		Author reported
	Amphipod Hyalella azteca		95		61		051		Biomass (28 d)			1993			101						Hyalella			Author reported
	Amphipod Hyalella azteca		106		604		033		Biomass (28 d)			425			289			171		171			Hyalella		Invert		Author reported
	Midge Chironomus riparius		118		558		18		Adult midge emergence			2955			119						Chironomus			Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Midge Chironomus riparius		119		505		18		Adult midge emergence			8442			2258						Chironomus			Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Midge Chironomus riparius		91		66		051		Reproduction ( of eggscase)			3387			766			591		591			Chironomus		Invert		Author reported
	Atlantic salmon Salmo salar		127		57		18		Biomass			6156			1112			1112		1112			Salmo		Fish		Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Brook trout Salvelinus fontinalis		123		655		19		Biomass			1644			59						Salvelinus			Author reported		wellRO mix
	Brook trout Salvelinus fontinalis		128		565		18		Biomass			1435			283			1293		1293			Salvelinus		Fish		Author reported		wellRO mix
	Fathead minnow Pimephales promelas		220		770		16		Biomass			6194			781						Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		well water
	Fathead minnow Pimephales promelas		96		620		025		Fry survival			4286			379			544		544			Pimephales		Fish		Author reported half the detection limit
	Zebrafish Danio rerio		83		61		025		Biomass			2344			241			241		241			Danio		Fish		Author reported half the detection limit
	Wood frog Rana sylvatica		115		469		16		MATC-survival		gt		2000		gt		2131		gt		2131		2131		gt		Rana		Amphib - Other Data		Value is from 2007 FW Cu AWQC Appendix C recommendation		aged tap water

	Appendix A Acceptable Acute Toxicity Data of Aluminum to Freshwater Aquatic Animals
	Species		Methoda		Hardness (mgL as CaCO3)		pH		DOC(mgL)			LC50 or EC50 Conc (microgL)		Reference			Normalized Conc - LC50(microgL)			Species Mean Acute Value(microgL)		Genus Mean Acute Value(microgL)			Genus			DOC Note		Dilution water
	Snail Physa sp		S M T		474		659		11		gt		23400		Call 1984 Call et al 1984		gt		4843			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		755		11			30600		Call 1984 Call et al 1984			2699			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		817		11		gt		24700		Call 1984 Call et al 1984		gt		2404			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		746		11			55500		Call 1984 Call et al 1984			5034			3546		3546			Physa		Mollusk		Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Fatmucket Lampsilis siliquoidea		F M T		1060		612		048		gt		6302		Wang et al 2016 Wang et al 2017		gt		2983		gt		2983		2983		gt		Lampsilis		Mollusk		Author reported
	Cladoceran Ceriodaphnia dubia		S M A		500		742		11			1900		McCauley et al 1986			173			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		S M A		505		786		11			1500		McCauley et al 1986			129			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		UW-S Lake Superior lab water
	Cladoceran Ceriodaphnia dubia		S M A		500		813		11			2560		McCauley et al 1986			243			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		R M T		26		75		05			720		ENSR 1992d			111			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		R M T		46		76		05			1880		ENSR 1992d			250			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		R M T		96		78		05			2450		ENSR 1992d			289			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		S U NR		90		715		05			3727		Fort and Stover 1995			500			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U NR		90		715		05			5673		Fort and Stover 1995			761			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U NR		89		76		05			2880		Soucek et al 2001			336			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		601		05			7112		European Al Association 2009			169			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		605		2			6865		European Al Association 2009			709			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		609		4			15581		European Al Association 2009			1007			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		601		05			681		European Al Association 2009			162			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		603		05			1630		European Al Association 2009			367			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		597		05			1785		European Al Association 2009			473			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		592		05			1410		European Al Association 2009			429			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		699		05		gt		1300		European Al Association 2009		gt		419			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		785		05		gt		5000		European Al Association 2009		gt		796			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		68		2		gt		10000		European Al Association 2009		gt		2076			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		782		2		gt		15000		European Al Association 2009		gt		1163			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		677		4		gt		10000		European Al Association 2009		gt		1516			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		766		4		gt		15000		European Al Association 2009		gt		861			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		79		05		gt		2000		European Al Association 2009		gt		315			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		789		05		gt		2000		European Al Association 2009		gt		316			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		604		05			1108		European Al Association 2009			85			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		598		2			11371		European Al Association 2009			482			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		573		4			80467		European Al Association 2009			4357			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		671		05		gt		10000		European Al Association 2009		gt		2379			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		783		05		gt		5000		European Al Association 2009		gt		634			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		679		2		gt		10000		European Al Association 2009		gt		1039			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		767		2		gt		15000		European Al Association 2009		gt		908			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		668		4		gt		15000		European Al Association 2009		gt		1246			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		762		4		gt		15000		European Al Association 2009		gt		633			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		606		2			33868		European Al Association 2009			792			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		56		4			104842		European Al Association 2009			4880			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		693		05		gt		5000		European Al Association 2009		gt		713			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		120		788		05		gt		5000		European Al Association 2009		gt		590			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		120		676		2		gt		15000		European Al Association 2009		gt		1212			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		771		2		gt		15000		European Al Association 2009		gt		810			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		66		4		gt		15000		European Al Association 2009		gt		1014			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		76		4		gt		15000		European Al Association 2009		gt		555			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			11971		European Al Association 2010			270			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			27478		European Al Association 2010			619			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			11998		European Al Association 2010			270			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		607		05			92495		European Al Association 2010			188			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		609		05			31337		European Al Association 2010			604			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		61		05			33235		European Al Association 2010			624			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		708		05		gt		8864		European Al Association 2010		gt		253			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		779		05		gt		42783		European Al Association 2010		gt		693			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		753		05			13204		European Al Association 2010			24			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		600		601		05			46326		European Al Association 2010			380			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		600		599		05		gt		8590		European Al Association 2010		gt		737			509				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia reticulata		F M T		451		60		11			304		Shephard 1983			201			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia reticulata		F M T		40		55		11			362		Shephard 1983			5531			1054		733			Ceriodaphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake SuperiorDI Mix
	Cladoceran Daphnia magna		S U NR		453		774		11			3900		Biesinger and Christensen 1972			338			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Daphnia magna		S M T		220		76		16			38200		Kimball 1978			2030			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Well water
	Cladoceran Daphnia magna		S U T		451		725		11			2800		Shephard 1983			287			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Daphnia magna		S U T		168		599		05			5000		European Al Association 2009		gt		227			-				Daphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Daphnia magna		S U T		168		792		05			7950		European Al Association 2009			92			333		333			Daphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Ostracod Stenocypris major		R M T		1563		651		16			310196		Shuhaimi-Othman et al 2011a			1022			1022		1022			Stenocypris		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (Malaysia)
	Amphipod Crangonyx pseudogracilis		R U T		50		675		16			9190		Martin and Holdich 1986			1217			1217		1217			Crangonyx		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (UK)
	Amphipod Hyalella azteca		F M T		105		613		048		gt		5997		Wang et al 2016 Wang et al 2017		gt		2800		gt		2800		2800		gt		Hyalella		Invert		Author reported
	Stonefly Acroneuria sp		S M T		474		746		11		gt		22600		Call et al 1984		gt		2050		gt		2050		2050		gt		Acroneuria		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Midge Chironomus plumosus		S U T		80		70		16			30000		Fargasova 2001			2536			2536		2536			Chironomus		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (Slovakia)
	Midge Paratanytarsus dissimilis		S M T		1743		728		28		gt		77700		Lamb and Bailey 1981 1983		gt		6232		gt		6232		6232		gt		Paratanytarsus		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Liberty Lake Washington
	Rainbow trout Oncorhynchus mykiss		F M T		274		758		05		gt		9840		Gundersen et al 1994		gt		1256			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		452		762		05		gt		8070		Gundersen et al 1994		gt		1140			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		895		758		05		gt		8160		Gundersen et al 1994		gt		1489			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		1304		758		05		gt		8200		Gundersen et al 1994		gt		1676			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		232		825		05			6170		Gundersen et al 1994			260			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		350		826		05			6170		Gundersen et al 1994			333			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		836		829		05			7670		Gundersen et al 1994			701			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		1158		830		05			6930		Gundersen et al 1994			777			801		801			Oncorhynchus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Atlantic salmon Salmo salar		S U T		68		55		05			584		Hamilton and Haines 1995			4602			-				Salmo			Value is from 2007 FW Cu AWQC Appendix C recommendation		Soft recon
	Atlantic salmon Salmo salar		S U T		68		65		05			599		Hamilton and Haines 1995			531			1564		1564			Salmo		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Soft recon
	Brook trout Salvelinus fontinalis		S U T		40		56		16			6530		Tandjung 1982			6947			-				Salvelinus			Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Brook trout Salvelinus fontinalis		S U T		18		56		16			3400		Tandjung 1982			6209			-				Salvelinus			Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Brook trout Salvelinus fontinalis		S U T		2		56		16			370		Tandjung 1982			2988			5051		5051			Salvelinus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Green sunfish Lepomis cyanellus		S M T		474		755		11		gt		50000		Call et al 1984		gt		5255		gt		5255		5255		gt		Lepomis		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Rio Grande silvery minnow Hybognathus amarus		R M T		140		81		05		gt		59100		Buhl 2002		gt		8620		gt		8620		8620		gt		Hybognathus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon water
	Fathead minnow Pimephales promelas		S M T		474		761		11		gt		48200		Call et al 1984		gt		4707			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Fathead minnow Pimephales promelas		S M T		474		805		11		gt		49800		Call et al 1984		gt		2837			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Fathead minnow Pimephales promelas		R M T		26		78		05			1160		ENSR 1992c			104			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		46		76		05			8180		ENSR 1992c			1191			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		96		81		05			20300		ENSR 1992c			2398			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		194		81		05			44800		ENSR 1992c			7839			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		140		81		05		gt		59100		Buhl 2002		gt		8620			2224		2224			Pimephales		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon water
	Smallmouth bass Micropterus dolomieui		S M T		1245		505		16			130		Kane and Rabeni 1987			859			859		859			Micropterus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Green tree frog Hyla cinerea		R M T		455		549		05		gt		4052		Jung and Jagoe 1995		gt		4377		gt		4377		4377		gt		Hyla		Amphibian		Value is from 2007 FW Cu AWQC Appendix C recommendation		artificial soft recon

	Normalization Chemistry
	pH		6					FAV		360
	Hardness		25					CMC		180
	DOC		1					CCC		99
	Acute Ranked GMAV											Chronic Ranked GMCV
	Rank			GMAV		Genus								Rank			GMCV		Genus
	18		gt		8620		Hybognathus		Fish							12		gt		2131		Rana		Amphib - Other Data
	17		gt		6232		Paratanytarsus		Invert							11			1710		Aeolosoma		Invert
	16		gt		5255		Lepomis		Fish							10			591		Chironomus		Invert
	15			5051		Salvelinus		Fish							9			544		Pimephales		Fish
	14		gt		4377		Hyla		Amphibian							8			488		Lymnaea		Mollusk
	13			3546		Physa		Mollusk							7			255		Brachionus		Invert
	12		gt		2983		Lampsilis		Mollusk							6			241		Danio		Fish
	11		gt		2800		Hyalella		Invert							5			171		Hyalella		Invert
	10			2536		Chironomus		Invert							4			129		Salvelinus		Fish
	9			2224		Pimephales		Fish							3			118		Ceriodaphnia		Invert
	8		gt		2050		Acroneuria		Invert							2			111		Salmo		Fish
	7			1564		Salmo		Fish							1			104		Lampsilis		Mollusk
	6			1217		Crangonyx		Invert
	5			1022		Stenocypris		Invert
	4			859		Micropterus		Fish
	3			801		Oncorhynchus		Fish
	2			733		Ceriodaphnia		Invert
	1			333		Daphnia		Invert
		N		Rank		Genus		GMAV		ln(GMAV)		ln(GMAV)2		P=R(N+1)		sqrt(P)				N		Rank		Genus		GMCV		ln(GMCV)		ln(GMCV)2		P=R(N+1)		sqrt(P)
		18		4		Micropterus		859		676		4564		0211		0459				12		4		Salvelinus		129		486		2364		0308		0555
			3		Oncorhynchus		801		669		4469		0158		0397					3		Ceriodaphnia		118		477		2277		0231		0480
			2		Ceriodaphnia		733		660		4352		0105		0324					2		Salmo		111		471		2220		0154		0392
			1		Daphnia		333		581		3374		0053		0229					1		Lampsilis		104		465		2159		0077		0277
			Sum				2585		1676		0526		141					Sum				1899		902		0769		170
			S2 =		1987										S2 =		059
			L =		4890										L =		4420
			A =		5887										A =		4592
			FAV =		360										FCV =		987
			CMC =		180

				All concentrations reported are microgL total Aluminum
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	ENTER DATA HERE									ERRORNUM		ACUTE														CHRONIC
										Rank 1				Rank 2				Rank 3				Rank 4					Rank 1				Rank 2				Rank 3				Rank 4
	SiteName		DOC(mgL)		Hardness(mgL as CaCO3)		pH			FAV		CMC		CCC			Genus		GMAV			Genus		GMAV			Genus		GMAV			Genus		GMAV				Genus		GMCV			Genus		GMCV			Genus		GMCV			Genus		GMCV
	Site 1		50		25		9		75428737034162		35229324666008		1800		770			Daphnia			33452		Ceriodaphnia			73576		Stenocypris			102576		Crangonyx			122230			Lampsilis			10463		Ceriodaphnia			11865		Hyalella			17200		Brachionus			25648
	Site 2		50		50		9		75428737034162		31204991509063		1600		680			Daphnia			29631		Ceriodaphnia			65171		Stenocypris			90859		Crangonyx			108267			Lampsilis			9268		Ceriodaphnia			10510		Hyalella			15236		Brachionus			22718
	Site 3		50		75		9		75428737034162		29067527928199		1500		640			Daphnia			27601		Ceriodaphnia			60707		Stenocypris			84635		Crangonyx			100851			Lampsilis			8633		Ceriodaphnia			9790		Hyalella			14192		Brachionus			21162
	Site 4		50		100		9		75428737034162		27640367912592		1400		600			Daphnia			26246		Ceriodaphnia			57726		Stenocypris			80480		Crangonyx			95899			Lampsilis			8209		Ceriodaphnia			9309		Hyalella			13495		Brachionus			20123
	Site 5		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 6		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 7		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 8		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 9		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 10		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 11		50		25		85		75428737034162		63245275664384		3200		1400			Daphnia			60055		Ceriodaphnia			132086		Stenocypris			184149		Crangonyx			219432			Lampsilis			18783		Ceriodaphnia			21301		Hyalella			30879		Brachionus			46045
	Site 12		50		50		85		75428737034162		61388044609926		3100		1300			Daphnia			58291		Ceriodaphnia			128208		Stenocypris			178742		Crangonyx			212988			Lampsilis			18232		Ceriodaphnia			20676		Hyalella			29972		Brachionus			44693
	Site 13		50		75		85		75428737034162		60327020429132		3000		1300			Daphnia			57284		Ceriodaphnia			125992		Stenocypris			175652		Crangonyx			209307			Lampsilis			17917		Ceriodaphnia			20318		Hyalella			29454		Brachionus			43920
	Site 14		50		100		85		75428737034162		59585352130146		3000		1300			Daphnia			56580		Ceriodaphnia			124443		Stenocypris			173493		Crangonyx			206734			Lampsilis			17696		Ceriodaphnia			20068		Hyalella			29092		Salmo			41264
	Site 15		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 16		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 17		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 18		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 19		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 20		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370

	Appendix C Acceptable Chronic Toxicity Data of Aluminum to Freshwater Aquatic Animals
									MLR equation
	Species		Hardness (mgL as CaCO3)		pH		DOC(mgL)		EC20 Endpoint			EC20(microgL)			Normalized Conc- EC20(microgL)			SMCV-EC20(microgL)		GMCV-EC20(microgL)			Genus			DOC Notes		Dilution water
	Oligochaete Aeolosoma sp		48		6		025		Reproduction (population size)			1235			17098			17098		17098			Aeolosoma		Invert		Author reported half the detection limit
	Rotifer Brachionus calyciflorus		100		62		025		Reproduction (population size)			431			2555			2555		2555			Brachionus		Invert		Author reported half the detection limit
	Great pond snail Lymnaea stagnalis		117		61		025		Biomass			746			4877			4877		4877			Lymnaea		Mollusk		Author reported half the detection limit
	Fatmucket Lampsilis siliquoidea		1055		604		040		Biomass			169			1042			1042		1042			Lampsilis		Mollusk		Author reported
	Cladoceran Ceriodaphnia dubia		50		715		11		Reproduction - youngstarting adult			1780			1898						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		26		73		05		Reproduction - youngfemale			1557			2718						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		46		75		05		Reproduction - youngfemale			8087			1102						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		96		79		05		Reproduction - youngfemale			6519			796						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		194		81		05		Reproduction - youngfemale			6836			888						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		255		703		05		Reproduction (youngfemale)			250			563						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		122		713		05		Reproduction (youngfemale)			860			1061						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		25		798		05		Reproduction (youngfemale)			700			1006						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		61		803		05		Reproduction (youngfemale)			1010			1363						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		122		810		05		Reproduction (youngfemale)			870			1164						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		255		633		05		Reproduction (youngfemale)			260			1758						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		121		636		05		Reproduction (youngfemale)			390			1101			1182		1182			Ceriodaphnia		Invert		Author reported
	Amphipod Hyalella azteca		95		61		051		Biomass (28 d)			1993			1014						Hyalella			Author reported
	Amphipod Hyalella azteca		106		604		033		Biomass (28 d)			425			2895			1713		1713			Hyalella		Invert		Author reported
	Midge Chironomus riparius		118		558		18		Adult midge emergence			2955			1192						Chironomus			Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Midge Chironomus riparius		119		505		18		Adult midge emergence			8442			22578						Chironomus			Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Midge Chironomus riparius		91		66		051		Reproduction ( of eggscase)			3387			7664			5908		5908			Chironomus		Invert		Author reported
	Atlantic salmon Salmo salar		127		57		18		Biomass			6156			5085			5085		5085			Salmo		Fish		Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Brook trout Salvelinus fontinalis		123		655		19		Biomass			1644			270						Salvelinus			Author reported		wellRO mix
	Brook trout Salvelinus fontinalis		128		565		18		Biomass			1435			1295			5914		5914			Salvelinus		Fish		Author reported		wellRO mix
	Fathead minnow Pimephales promelas		220		770		16		Biomass			6194			3569						Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		well water
	Fathead minnow Pimephales promelas		96		620		025		Fry survival			4286			1734			2488		2488			Pimephales		Fish		Author reported half the detection limit
	Zebrafish Danio rerio		83		61		025		Biomass			2344			1102			1102		1102			Danio		Fish		Author reported half the detection limit
	Wood frog Rana sylvatica		115		469		16		MATC-survival		gt		2000		gt		9746		gt		9746		9746		gt		Rana		Amphib - Other Data		Value is from 2007 FW Cu AWQC Appendix C recommendation		aged tap water

	Appendix A Acceptable Acute Toxicity Data of Aluminum to Freshwater Aquatic Animals
	Species		Methoda		Hardness (mgL as CaCO3)		pH		DOC(mgL)			LC50 or EC50 Conc (microgL)		Reference			Normalized Conc - LC50(microgL)			Species Mean Acute Value(microgL)		Genus Mean Acute Value(microgL)			Genus			DOC Note		Dilution water
	Snail Physa sp		S M T		474		659		11		gt		23400		Call 1984 Call et al 1984		gt		48425			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		755		11			30600		Call 1984 Call et al 1984			26986			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		817		11		gt		24700		Call 1984 Call et al 1984		gt		24040			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		746		11			55500		Call 1984 Call et al 1984			50338			35462		35462			Physa		Mollusk		Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Fatmucket Lampsilis siliquoidea		F M T		1060		612		048		gt		6302		Wang et al 2016 Wang et al 2017		gt		29834		gt		29834		29834		gt		Lampsilis		Mollusk		Author reported
	Cladoceran Ceriodaphnia dubia		S M A		500		742		11			1900		McCauley et al 1986			1728			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		S M A		505		786		11			1500		McCauley et al 1986			1294			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		UW-S Lake Superior lab water
	Cladoceran Ceriodaphnia dubia		S M A		500		813		11			2560		McCauley et al 1986			2431			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		R M T		26		75		05			720		ENSR 1992d			1113			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		R M T		46		76		05			1880		ENSR 1992d			2495			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		R M T		96		78		05			2450		ENSR 1992d			2894			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		S U NR		90		715		05			3727		Fort and Stover 1995			5002			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U NR		90		715		05			5673		Fort and Stover 1995			7613			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U NR		89		76		05			2880		Soucek et al 2001			3362			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		601		05			7112		European Al Association 2009			1691			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		605		2			6865		European Al Association 2009			7088			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		609		4			15581		European Al Association 2009			10072			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		601		05			681		European Al Association 2009			1620			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		603		05			1630		European Al Association 2009			3674			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		597		05			1785		European Al Association 2009			4732			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		592		05			1410		European Al Association 2009			4295			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		699		05		gt		1300		European Al Association 2009		gt		4189			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		785		05		gt		5000		European Al Association 2009		gt		7960			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		68		2		gt		10000		European Al Association 2009		gt		20755			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		782		2		gt		15000		European Al Association 2009		gt		11626			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		677		4		gt		10000		European Al Association 2009		gt		15161			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		766		4		gt		15000		European Al Association 2009		gt		8608			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		79		05		gt		2000		European Al Association 2009		gt		3150			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		789		05		gt		2000		European Al Association 2009		gt		3156			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		604		05			1108		European Al Association 2009			850			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		598		2			11371		European Al Association 2009			4817			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		573		4			80467		European Al Association 2009			43570			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		671		05		gt		10000		European Al Association 2009		gt		23786			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		783		05		gt		5000		European Al Association 2009		gt		6344			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		679		2		gt		10000		European Al Association 2009		gt		10394			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		767		2		gt		15000		European Al Association 2009		gt		9082			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		668		4		gt		15000		European Al Association 2009		gt		12461			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		762		4		gt		15000		European Al Association 2009		gt		6332			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		606		2			33868		European Al Association 2009			7920			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		56		4			104842		European Al Association 2009			48800			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		693		05		gt		5000		European Al Association 2009		gt		7126			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		120		788		05		gt		5000		European Al Association 2009		gt		5898			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		120		676		2		gt		15000		European Al Association 2009		gt		12117			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		771		2		gt		15000		European Al Association 2009		gt		8096			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		66		4		gt		15000		European Al Association 2009		gt		10139			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		76		4		gt		15000		European Al Association 2009		gt		5545			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			11971		European Al Association 2010			2699			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			27478		European Al Association 2010			6194			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			11998		European Al Association 2010			2705			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		607		05			92495		European Al Association 2010			1876			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		609		05			31337		European Al Association 2010			6035			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		61		05			33235		European Al Association 2010			6238			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		708		05		gt		8864		European Al Association 2010		gt		2534			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		779		05		gt		42783		European Al Association 2010		gt		6930			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		753		05			13204		European Al Association 2010			244			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		600		601		05			46326		European Al Association 2010			3798			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		600		599		05		gt		8590		European Al Association 2010		gt		7366			5094				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia reticulata		F M T		451		60		11			304		Shephard 1983			2009			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia reticulata		F M T		40		55		11			362		Shephard 1983			55307			10542		7328			Ceriodaphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake SuperiorDI Mix
	Cladoceran Daphnia magna		S U NR		453		774		11			3900		Biesinger and Christensen 1972			3382			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Daphnia magna		S M T		220		76		16			38200		Kimball 1978			20303			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Well water
	Cladoceran Daphnia magna		S U T		451		725		11			2800		Shephard 1983			2868			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Daphnia magna		S U T		168		599		05			5000		European Al Association 2009		gt		2265			-				Daphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Daphnia magna		S U T		168		792		05			7950		European Al Association 2009			920			3332		3332			Daphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Ostracod Stenocypris major		R M T		1563		651		16			310196		Shuhaimi-Othman et al 2011a			10216			10216		10216			Stenocypris		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (Malaysia)
	Amphipod Crangonyx pseudogracilis		R U T		50		675		16			9190		Martin and Holdich 1986			12174			12174		12174			Crangonyx		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (UK)
	Amphipod Hyalella azteca		F M T		105		613		048		gt		5997		Wang et al 2016 Wang et al 2017		gt		28002		gt		28002		28002		gt		Hyalella		Invert		Author reported
	Stonefly Acroneuria sp		S M T		474		746		11		gt		22600		Call et al 1984		gt		20498		gt		20498		20498		gt		Acroneuria		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Midge Chironomus plumosus		S U T		80		70		16			30000		Fargasova 2001			25361			25361		25361			Chironomus		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (Slovakia)
	Midge Paratanytarsus dissimilis		S M T		1743		728		28		gt		77700		Lamb and Bailey 1981 1983		gt		62318		gt		62318		62318		gt		Paratanytarsus		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Liberty Lake Washington
	Rainbow trout Oncorhynchus mykiss		F M T		274		758		05		gt		9840		Gundersen et al 1994		gt		5745			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		452		762		05		gt		8070		Gundersen et al 1994		gt		5212			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		895		758		05		gt		8160		Gundersen et al 1994		gt		6807			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		1304		758		05		gt		8200		Gundersen et al 1994		gt		7662			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		232		825		05			6170		Gundersen et al 1994			1190			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		350		826		05			6170		Gundersen et al 1994			1523			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		836		829		05			7670		Gundersen et al 1994			3205			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		1158		830		05			6930		Gundersen et al 1994			3554			3661		3661			Oncorhynchus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Atlantic salmon Salmo salar		S U T		68		55		05			584		Hamilton and Haines 1995			21042			-				Salmo			Value is from 2007 FW Cu AWQC Appendix C recommendation		Soft recon
	Atlantic salmon Salmo salar		S U T		68		65		05			599		Hamilton and Haines 1995			2430			7151		7151			Salmo		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Soft recon
	Brook trout Salvelinus fontinalis		S U T		40		56		16			6530		Tandjung 1982			31767			-				Salvelinus			Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Brook trout Salvelinus fontinalis		S U T		18		56		16			3400		Tandjung 1982			28391			-				Salvelinus			Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Brook trout Salvelinus fontinalis		S U T		2		56		16			370		Tandjung 1982			13663			23097		23097			Salvelinus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Green sunfish Lepomis cyanellus		S M T		474		755		11		gt		50000		Call et al 1984		gt		24028		gt		24028		24028		gt		Lepomis		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Rio Grande silvery minnow Hybognathus amarus		R M T		140		81		05		gt		59100		Buhl 2002		gt		39414		gt		39414		39414		gt		Hybognathus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon water
	Fathead minnow Pimephales promelas		S M T		474		761		11		gt		48200		Call et al 1984		gt		21522			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Fathead minnow Pimephales promelas		S M T		474		805		11		gt		49800		Call et al 1984		gt		12972			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Fathead minnow Pimephales promelas		R M T		26		78		05			1160		ENSR 1992c			477			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		46		76		05			8180		ENSR 1992c			5446			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		96		81		05			20300		ENSR 1992c			10966			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		194		81		05			44800		ENSR 1992c			35847			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		140		81		05		gt		59100		Buhl 2002		gt		39414			10168		10168			Pimephales		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon water
	Smallmouth bass Micropterus dolomieui		S M T		1245		505		16			130		Kane and Rabeni 1987			3929			3929		3929			Micropterus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Green tree frog Hyla cinerea		R M T		455		549		05		gt		4052		Jung and Jagoe 1995		gt		20016		gt		20016		20016		gt		Hyla		Amphibian		Value is from 2007 FW Cu AWQC Appendix C recommendation		artificial soft recon

	Normalization Chemistry
	pH		7					FAV		2741
	Hardness		100					CMC		1400
	DOC		1					CCC		390
	Acute Ranked GMAV											Chronic Ranked GMCV
	Rank			GMAV		Genus								Rank			GMCV		Genus
	18		gt		62318		Paratanytarsus		Invert							12			17098		Aeolosoma		Invert
	17		gt		39414		Hybognathus		Fish							11		gt		9746		Rana		Amphib - Other Data
	16			35462		Physa		Mollusk							10			5908		Chironomus		Invert
	15		gt		29834		Lampsilis		Mollusk							9			4877		Lymnaea		Mollusk
	14		gt		28002		Hyalella		Invert							8			2555		Brachionus		Invert
	13			25361		Chironomus		Invert							7			2488		Pimephales		Fish
	12		gt		24028		Lepomis		Fish							6			1713		Hyalella		Invert
	11			23097		Salvelinus		Fish							5			1182		Ceriodaphnia		Invert
	10		gt		20498		Acroneuria		Invert							4			1102		Danio		Fish
	9		gt		20016		Hyla		Amphibian							3			1042		Lampsilis		Mollusk
	8			12174		Crangonyx		Invert							2			591		Salvelinus		Fish
	7			10216		Stenocypris		Invert							1			509		Salmo		Fish
	6			10168		Pimephales		Fish
	5			7328		Ceriodaphnia		Invert
	4			7151		Salmo		Fish
	3			3929		Micropterus		Fish
	2			3661		Oncorhynchus		Fish
	1			3332		Daphnia		Invert
		N		Rank		Genus		GMAV		ln(GMAV)		ln(GMAV)2		P=R(N+1)		sqrt(P)				N		Rank		Genus		GMCV		ln(GMCV)		ln(GMCV)2		P=R(N+1)		sqrt(P)
		18		4		Salmo		7151		887		7876		0211		0459				12		4		Danio		1102		700		4907		0308		0555
			3		Micropterus		3929		828		6849		0158		0397					3		Lampsilis		1042		695		4829		0231		0480
			2		Oncorhynchus		3661		821		6733		0105		0324					2		Salvelinus		591		638		4074		0154		0392
			1		Daphnia		3332		811		6579		0053		0229					1		Salmo		509		623		3883		0077		0277
			Sum				3347		2804		0526		141					Sum				2657		1769		0769		170
			S2 =		1223										S2 =		1080
			L =		7134										L =		5242
			A =		7916										A =		5976
			FAV =		2741										FCV =		3940
			CMC =		1371

				All concentrations reported are microgL total Aluminum
				lt-----Click Button after entering data
	ENTER DATA HERE									ERRORNUM		ACUTE														CHRONIC
										Rank 1				Rank 2				Rank 3				Rank 4					Rank 1				Rank 2				Rank 3				Rank 4
	SiteName		DOC(mgL)		Hardness(mgL as CaCO3)		pH			FAV		CMC		CCC			Genus		GMAV			Genus		GMAV			Genus		GMAV			Genus		GMAV				Genus		GMCV			Genus		GMCV			Genus		GMCV			Genus		GMCV
	Site 1		50		25		9		75428737034162		35229324666008		1800		770			Daphnia			33452		Ceriodaphnia			73576		Stenocypris			102576		Crangonyx			122230			Lampsilis			10463		Ceriodaphnia			11865		Hyalella			17200		Brachionus			25648
	Site 2		50		50		9		75428737034162		31204991509063		1600		680			Daphnia			29631		Ceriodaphnia			65171		Stenocypris			90859		Crangonyx			108267			Lampsilis			9268		Ceriodaphnia			10510		Hyalella			15236		Brachionus			22718
	Site 3		50		75		9		75428737034162		29067527928199		1500		640			Daphnia			27601		Ceriodaphnia			60707		Stenocypris			84635		Crangonyx			100851			Lampsilis			8633		Ceriodaphnia			9790		Hyalella			14192		Brachionus			21162
	Site 4		50		100		9		75428737034162		27640367912592		1400		600			Daphnia			26246		Ceriodaphnia			57726		Stenocypris			80480		Crangonyx			95899			Lampsilis			8209		Ceriodaphnia			9309		Hyalella			13495		Brachionus			20123
	Site 5		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 6		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 7		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 8		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 9		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 10		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 11		50		25		85		75428737034162		63245275664384		3200		1400			Daphnia			60055		Ceriodaphnia			132086		Stenocypris			184149		Crangonyx			219432			Lampsilis			18783		Ceriodaphnia			21301		Hyalella			30879		Brachionus			46045
	Site 12		50		50		85		75428737034162		61388044609926		3100		1300			Daphnia			58291		Ceriodaphnia			128208		Stenocypris			178742		Crangonyx			212988			Lampsilis			18232		Ceriodaphnia			20676		Hyalella			29972		Brachionus			44693
	Site 13		50		75		85		75428737034162		60327020429132		3000		1300			Daphnia			57284		Ceriodaphnia			125992		Stenocypris			175652		Crangonyx			209307			Lampsilis			17917		Ceriodaphnia			20318		Hyalella			29454		Brachionus			43920
	Site 14		50		100		85		75428737034162		59585352130146		3000		1300			Daphnia			56580		Ceriodaphnia			124443		Stenocypris			173493		Crangonyx			206734			Lampsilis			17696		Ceriodaphnia			20068		Hyalella			29092		Salmo			41264
	Site 15		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 16		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 17		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 18		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 19		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 20		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370

Current 1988 Aluminum Criteriabull The final 1988 criteria document recommended

ndash An acute criterion of 750 microgL total aluminum when the pH is between 65 and 90

ndash Chronic criterion of 87 microgL total aluminum when the pH is between 65 and 90

ndash The 1988 freshwater chronic criterion of 87 microgL was lowered based on two tests (brook trout and striped bass)

ndash The studies were not pH adjusted and all the data were not normalized for hardness The 1988 existing criteria did not take hardness and DOC into account

6

Aluminum Standardsbull Aluminum Standards

ndash About 20 states have numeric criteria for aluminum for aquatic life

bull Most are based on EPArsquos 1988 values and two are hardness-based equations (CO and NM)

bull There are numerous site-specific criteria that involve the Biotic Ligand Model (BLM) or a Water Effects Ratio (WER) -adjusted 1988 aluminum criteria

ndash Aluminum is not a priority pollutant

7

Criteria Development Processbull All EPA Aquatic Life Criteria for Toxics undergo rigorous

scientific development and review processbull Develop criteria document draftbull Intra-agency peer review process bull Independent external peer reviewbull Revisions based on external peer review

Intra-agency review of draft criteria documentbull Release to the public in draft form to obtain scientific views

Revisions considering public comments Intra-agency review of final criteria document

bull Publication of final criteria document bull States may then consider recommended criteria in

development and adoption of state water quality standards8

Framework for Updated Draft Aluminum Criteria

I Screen toxicological studies for high

quality data

II Consider water conditions effects on

aluminum bioavailability

III Normalize toxicity data in species

sensitivity distribution to a set of water quality conditions

IV Construct sensitivity distribution to determine criteria

at set conditions

9

I Screen toxicological studies for high quality data

bull First step in all criteria developmentndash Data are evaluated based on the data quality objectives

outlined in the 1985 Guidelines methodology and other toxicity testing recommendations (ie EPA ASTM)

ndash High quality data are used quantitatively to develop criteria magnitudes

ndash Lower quality data are used qualitatively if appropriatendash Lowest quality data are considered unacceptable and not

usedbull Data are grouped into acute and chronic exposures

10

Aluminum Toxicity Databull The updated freshwater aluminum criteria meets the

acute and chronic minimum data requirements outlined in EPArsquos 1985 Guidelines

InvertebrateSpecies

Fish Species

AmphibianSpecies

Total Genera

Acute1988 AWQC 8 7 - 142017 Draft AWQC 11 8 1 18

Chronic1988 AWQC 2 1 - 32017 Draft AWQC 7 4 - 11

11 1988 AWQC Chronic CCC based on two studies for the striped

bass and brook trout

Aluminum Toxicity Data Freshwater mussels have been

suggested to be potentially sensitive to aluminum

EPA worked collaboratively with USGSrsquos Columbia Environmental Research Center (CERC) to have acute and chronic aluminum toxicity tests conducted with a unionid mussel (fatmucket Lampsilis siliquoidea) in water-only exposures to obtain additional data on invertebrates New data on amphipod (Hyalella azteca)

were collected to confirm laboratory conditions

12

Aluminum Toxicity Data

13

bull New data and models examinedndash Data submitted to EU REACH program

bull New freshwater aluminum toxicity data generated by Oregon State University

bull 2016 BLM model provided by the European Aluminum Association

ndash Saltwater data provided by Australia bull Insufficient data to develop estuarinemarine acute or chronic

criteria at this timendash Multiple Linear Regression (MLR) model developed by

DeForest et al 2017bull DeForest DK KV Brix LM Tear and WJ Adams 2017 Multiple Linear Regression (MLR) models for predicting chronic aluminum

toxicity to freshwater aquatic organisms and developing water quality guidelines Environ Toxicol Chem (DOI101002etc3922)

14

Minimum Dataset For Freshwater Criteria Derivation

SALMONID SECONDFISHFAMILY

CHORDATA

PLANKTONICCRUSTACEAN

BENTHIC CRUSTACEAN

INSECT ROTIFERA ANNELIDA MOLLUSCA

OTHERINSECT ORMOLLUSCA

EstuarineMarine Minimum Data Requirements

15

Family Minimum Data Requirement EstuarineMarine

Acute(Phylum Family Genus)

Chronic(Phylum Family Genus)

Family in the phylum Chordata - -

Family in the phylum Chordata - -

Either the Mysidae or Penaeidae family - -

Family in a phylum other than Arthropoda or Chordata Mollusca Ostreidae Crassostrea -

Family in a phylum other than Chordata Annelida Nereididae Neanthes -

Family in a phylum other than Chordata Annelida Capitellidae Capitella -

Family in a phylum other than Chordata Annelida Ctenodrilidae Ctenodrilus -

Any other family Arthropoda Ameiridae Nitokra -

II Evaluate water quality conditions to determine applicability

bull Criteria are often based on one or more water quality conditions (eg hardness) that affect toxicity of the material to test organisms

bull Aluminum toxicity is highly dependent on the pH of the test waterndash The pH of test waters affects the amount and form

of aluminum that is bioavailable to test speciesndash Hardness and DOC (Dissolved Organic Carbon)

also influence the amount of aluminum that is bioavailable

16

Multiple Bioavailability Modeling Approaches Underwent External Peer Review

17

bull Biotic Ligand Modelsbull Full aluminum BLM (2016 model version) ndash Temperature pH

Dissolved organic carbon (DOC) Major cations (calcium [Ca] magnesium [Mg] sodium [Na] and potassium [K]) Major anions (sulfate [SO4] and chloride [Cl]) Alkalinity and Sulfide

bull Simplified aluminum BLM - pH hardness DOC temperature

bull Multilinear Regression Equationsbull pH and Hardness

Accounting for Effects of Water Chemistry on Bioavailability and Toxicity

bull Water quality criteria can be modified quantitatively when enough data are available to demonstrate that those conditions similarly affect the results with a variety of speciesndash High quality data in the draft aluminum document demonstrated

that pH DOC and hardness affected a variety of species in a similar way when exposed to aluminum concentrations

bull Recent publications by Cardwell et al (2017) and Gensemer et al (2017) summarized short-term aluminum chronic toxicity data across a range of DOC pH and hardness values for three species

18

Multiple Linear Regression (MLR) Models to Account for Effects of Water Chemistry

bull MLR models were developed for each species (DeForest et al 2017)ndash 119862119862 119889119889119906119906119887119887119894119894119886119886 11986411986411986211986220=119890119890[minus41026+[0525timesln(119863119863119874119874119862119862)]+[2201timesln(ℎ119886119886119903119903119889119889)]+(11282times119901119901119867119867)minus(0633times1199011199011198671198672)minus[0264times119901119901119867119867ln(ℎ119886119886119903119903119889119889)]]

ndash 119875119875 119901119901119903119903119900119900119898119898119890119890119897119897119886119886119904119904 11986411986411986211986220=119890119890[minus14029+[0503timesln(119863119863119874119874119862119862)]+[3443timesln(ℎ119886119886119903119903119889119889)]+(3131times119901119901119867119867)minus[0494times119901119901119867119867ln(ℎ119886119886119903119903119889119889)]]

bull These models were applied to both the acute and chronic aluminum toxicity datandash The C dubia model was applied to invertebrate datandash The P promelas model was applied to vertebrate data

19

III Normalize toxicity data in species sensitivity distribution to a set of water

quality conditionsbull Just like single parameter hardness models the MLR

models were used to normalize the aluminum toxicity data to a single set of water quality conditionsndash As an illustration for one set of calculations the draft

aluminum AWQC set these conditions to pH 7 hardness of 100 mgL and DOC of 1 mgL

bull This allows for comparisons to be made between species based on their sensitivity to aluminumndash Apples to apples

20

IV Construct sensitivity distribution to determine criteria at set conditions

21

Figure 8 Ranked Summary of Total Aluminum Genus Mean Acute Values (GMAVs) - Freshwater at pH 7 Hardness of 100 mgL and DOC of 1 mgL

IV Construct sensitivity distribution to determine criteria at set conditions

22

Figure 10 Ranked Summary of Total Aluminum Genus Mean Chronic Values (GMCVs) ndash Freshwater Supplemented with Other Data to Fulfill Missing MDRs at pH 7 Hardness of 100 mgL and DOC of 1 mgL

Aluminum Criteria Calculatorbull The relative ranking of genera in the sensitivity

distribution to which the toxicity data are normalized will change based on the set of water chemistry conditions (ie pH hardness and DOC)

bull To make calculating aluminum criteria easier EPA developed an Aluminum Criteria Calculatorndash Automated so that user only needs to enter water chemistry

conditions of interestndash Allows quick calculations for a variety of conditionsndash Limits calculations to the recommended conditions for which

the MLR models were developed were specified for hardness and DOC

bull No extrapolation beyond hardness and DOC empirical data bounds

23

Aluminum Criteria Calculator SD Example

24

pH=7 Hardness=100 DOC=1 pH=6 Hardness=25 DOC=1

Chronic Ranked GMCVRank GMCV Genus

12 17098 Aeolosoma Invert11 gt 9746 Rana Amphib - Other Data10 5908 Chironomus Invert

9 4877 Lymnaea Mollusk8 2555 Brachionus Invert7 2488 Pimephales Fish6 1713 Hyalella Invert5 1182 Ceriodaphnia Invert4 1102 Danio Fish3 1042 Lampsilis Mollusk2 591 Salvelinus Fish1 509 Salmo Fish

FCV = 3940 microgL total aluminum

Chronic Ranked GMCVRank GMCV Genus

12 gt 2131 Rana Amphib - Other Data11 1710 Aeolosoma Invert10 591 Chironomus Invert

9 544 Pimephales Fish8 488 Lymnaea Mollusk7 255 Brachionus Invert6 241 Danio Fish5 171 Hyalella Invert4 129 Salvelinus Fish3 118 Ceriodaphnia Invert2 111 Salmo Fish1 104 Lampsilis Mollusk

FCV = 987 microgL total aluminum

Multiple Scenarios

Run Scenarios

Summary Sheet

Acute Dataset

Chronic Dataset

Multiple Scenarios

Run Scenarios

Summary Sheet

Acute Dataset

Chronic Dataset

Boundaries of the Criteria Outputsbull MLR models were developed using data that

encompassndash DOC range of 008-5 mgLndash Hardness range of 98-127 mgL (as CaCO3)ndash pH range of 6-81

bull MLR criteria outputs are bounded atndash Maximum of 150 mgL total hardness (as CaCO3)

ndash DOC of 50 mgLndash pH range of the model is 50 to 90

25

Aluminum Criteria Look-Up Tables

26

bull EPA also provided criteria look-up tables in Appendix K

Har

dnes

s

CCC(microgL total aluminum)

(DOC=05 mgL)

pH 50 pH 55 pH 60 pH 65 pH 70 pH 75 pH 80 pH 85 pH 90

25 23 a 14 b 69 c 160 f 350 f 620 i 540 b 410 a 230 a

50 43 a 24 b 100 d 170 f 310 f 630 f 670 j 400 a 200 a

75 61 a 33 b 130 e 180 f 290 f 530 f 730 i 390 a 190 a

100 78 a 41 b 150 e 190 f 280 g 460 f 770 c 390 a 180 a

150 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

200 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

250 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

300 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

350 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

400 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

Aluminum Criteria Look-Up Tables

27

bull EPA also provided criteria look-up tables in Appendix K

Har

dnes

s

CCC(microgL total aluminum)

(DOC=10 mgL)

pH 50 pH 55 pH 60 pH 65 pH 70 pH 75 pH 80 pH 85 pH 90

25 33 a 21 b 99 c 220 f 490 f 900 i 780 b 590 a 330 a

50 61 a 35 b 150 e 250 f 440 f 890 f 960 j 580 a 290 a

75 88 a 47 b 180 e 260 f 410 f 740 f 1100 i 570 a 270 a

100 11 a 59 b 210 e 270 f 390 g 650 f 1100 c 560 b 260 a

150 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

200 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

250 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

300 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

350 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

400 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

Aluminum Criteria Look-Up Tables

28

bull EPA also provided criteria look-up tables in Appendix K

Har

dnes

s

CCC(microgL total aluminum)

(DOC=25 mgL)

pH 50 pH 55 pH 60 pH 65 pH 70 pH 75 pH 80 pH 85 pH 90

25 54 a 34 b 160 c 350 f 770 f 1500 i 1300 b 960 a 540 a

50 99 a 57 b 230 e 390 f 690 f 1400 f 1600 j 930 a 470 a

75 14 a 77 b 290 e 410 f 640 f 1200 f 1700 i 920 a 440 a

100 18 a 96 b 330 e 420 f 620 g 1000 f 1800 d 920 b 420 a

150 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

200 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

250 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

300 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

350 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

400 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

Aluminum Criteria Look-Up Tables

29

bull EPA also provided criteria look-up tables in Appendix K

Har

dnes

s

CCC(microgL total aluminum)

(DOC=50 mgL)

pH 50 pH 55 pH 60 pH 65 pH 70 pH 75 pH 80 pH 85 pH 90

25 77 a 49 b 230 c 500 f 1100 f 2100 c 1900 b 1400 a 770 a

50 14 a 82 b 330 e 540 f 970 f 2000 f 2200 j 1300 a 680 a

75 20 a 110 b 410 e 570 f 900 f 1600 f 2500 i 1300 a 640 a

100 26 a 140 b 460 f 590 f 880 g 1400 f 2500 d 1300 b 600 a

150 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

200 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

250 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

300 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

350 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

400 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

Status

30

bull Draft Document Released to the Federal Register on 72817ndash Public comment period of 60 days ndash Due Date for Comments is 92617

bull Revisions will be made considering public commentsbull Subsequent EPA intra-agency review of final criteria

document will then be conducted bull Publication of final criteria document planned for Late Fall

2017

Federal Register Webpagehttpswwwfederalregistergovdocuments201

707282017-15968request-for-scientific-views-draft-updated-aquatic-life-ambient-water-quality-criteria-for-aluminum

Aluminum Criteria Webpagehttpswwwepagovwqc2017-draft-aquatic-life-

criteria-aluminum-freshwater-documents

31

	Appendix C Acceptable Chronic Toxicity Data of Aluminum to Freshwater Aquatic Animals
									MLR equation
	Species		Hardness (mgL as CaCO3)		pH		DOC(mgL)		EC20 Endpoint			EC20(microgL)			Normalized Conc- EC20(microgL)			SMCV-EC20(microgL)		GMCV-EC20(microgL)			Genus			DOC Notes		Dilution water
	Oligochaete Aeolosoma sp		48		6		025		Reproduction (population size)			1235			1710			1710		1710			Aeolosoma		Invert		Author reported half the detection limit
	Rotifer Brachionus calyciflorus		100		62		025		Reproduction (population size)			431			255			255		255			Brachionus		Invert		Author reported half the detection limit
	Great pond snail Lymnaea stagnalis		117		61		025		Biomass			746			488			488		488			Lymnaea		Mollusk		Author reported half the detection limit
	Fatmucket Lampsilis siliquoidea		1055		604		040		Biomass			169			104			104		104			Lampsilis		Mollusk		Author reported
	Cladoceran Ceriodaphnia dubia		50		715		11		Reproduction - youngstarting adult			1780			190						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		26		73		05		Reproduction - youngfemale			1557			272						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		46		75		05		Reproduction - youngfemale			8087			110						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		96		79		05		Reproduction - youngfemale			6519			80						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		194		81		05		Reproduction - youngfemale			6836			89						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		255		703		05		Reproduction (youngfemale)			250			56						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		122		713		05		Reproduction (youngfemale)			860			106						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		25		798		05		Reproduction (youngfemale)			700			101						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		61		803		05		Reproduction (youngfemale)			1010			136						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		122		810		05		Reproduction (youngfemale)			870			116						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		255		633		05		Reproduction (youngfemale)			260			176						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		121		636		05		Reproduction (youngfemale)			390			110			118		118			Ceriodaphnia		Invert		Author reported
	Amphipod Hyalella azteca		95		61		051		Biomass (28 d)			1993			101						Hyalella			Author reported
	Amphipod Hyalella azteca		106		604		033		Biomass (28 d)			425			289			171		171			Hyalella		Invert		Author reported
	Midge Chironomus riparius		118		558		18		Adult midge emergence			2955			119						Chironomus			Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Midge Chironomus riparius		119		505		18		Adult midge emergence			8442			2258						Chironomus			Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Midge Chironomus riparius		91		66		051		Reproduction ( of eggscase)			3387			766			591		591			Chironomus		Invert		Author reported
	Atlantic salmon Salmo salar		127		57		18		Biomass			6156			1112			1112		1112			Salmo		Fish		Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Brook trout Salvelinus fontinalis		123		655		19		Biomass			1644			59						Salvelinus			Author reported		wellRO mix
	Brook trout Salvelinus fontinalis		128		565		18		Biomass			1435			283			1293		1293			Salvelinus		Fish		Author reported		wellRO mix
	Fathead minnow Pimephales promelas		220		770		16		Biomass			6194			781						Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		well water
	Fathead minnow Pimephales promelas		96		620		025		Fry survival			4286			379			544		544			Pimephales		Fish		Author reported half the detection limit
	Zebrafish Danio rerio		83		61		025		Biomass			2344			241			241		241			Danio		Fish		Author reported half the detection limit
	Wood frog Rana sylvatica		115		469		16		MATC-survival		gt		2000		gt		2131		gt		2131		2131		gt		Rana		Amphib - Other Data		Value is from 2007 FW Cu AWQC Appendix C recommendation		aged tap water

	Appendix A Acceptable Acute Toxicity Data of Aluminum to Freshwater Aquatic Animals
	Species		Methoda		Hardness (mgL as CaCO3)		pH		DOC(mgL)			LC50 or EC50 Conc (microgL)		Reference			Normalized Conc - LC50(microgL)			Species Mean Acute Value(microgL)		Genus Mean Acute Value(microgL)			Genus			DOC Note		Dilution water
	Snail Physa sp		S M T		474		659		11		gt		23400		Call 1984 Call et al 1984		gt		4843			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		755		11			30600		Call 1984 Call et al 1984			2699			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		817		11		gt		24700		Call 1984 Call et al 1984		gt		2404			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		746		11			55500		Call 1984 Call et al 1984			5034			3546		3546			Physa		Mollusk		Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Fatmucket Lampsilis siliquoidea		F M T		1060		612		048		gt		6302		Wang et al 2016 Wang et al 2017		gt		2983		gt		2983		2983		gt		Lampsilis		Mollusk		Author reported
	Cladoceran Ceriodaphnia dubia		S M A		500		742		11			1900		McCauley et al 1986			173			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		S M A		505		786		11			1500		McCauley et al 1986			129			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		UW-S Lake Superior lab water
	Cladoceran Ceriodaphnia dubia		S M A		500		813		11			2560		McCauley et al 1986			243			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		R M T		26		75		05			720		ENSR 1992d			111			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		R M T		46		76		05			1880		ENSR 1992d			250			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		R M T		96		78		05			2450		ENSR 1992d			289			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		S U NR		90		715		05			3727		Fort and Stover 1995			500			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U NR		90		715		05			5673		Fort and Stover 1995			761			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U NR		89		76		05			2880		Soucek et al 2001			336			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		601		05			7112		European Al Association 2009			169			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		605		2			6865		European Al Association 2009			709			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		609		4			15581		European Al Association 2009			1007			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		601		05			681		European Al Association 2009			162			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		603		05			1630		European Al Association 2009			367			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		597		05			1785		European Al Association 2009			473			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		592		05			1410		European Al Association 2009			429			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		699		05		gt		1300		European Al Association 2009		gt		419			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		785		05		gt		5000		European Al Association 2009		gt		796			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		68		2		gt		10000		European Al Association 2009		gt		2076			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		782		2		gt		15000		European Al Association 2009		gt		1163			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		677		4		gt		10000		European Al Association 2009		gt		1516			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		766		4		gt		15000		European Al Association 2009		gt		861			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		79		05		gt		2000		European Al Association 2009		gt		315			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		789		05		gt		2000		European Al Association 2009		gt		316			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		604		05			1108		European Al Association 2009			85			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		598		2			11371		European Al Association 2009			482			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		573		4			80467		European Al Association 2009			4357			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		671		05		gt		10000		European Al Association 2009		gt		2379			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		783		05		gt		5000		European Al Association 2009		gt		634			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		679		2		gt		10000		European Al Association 2009		gt		1039			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		767		2		gt		15000		European Al Association 2009		gt		908			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		668		4		gt		15000		European Al Association 2009		gt		1246			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		762		4		gt		15000		European Al Association 2009		gt		633			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		606		2			33868		European Al Association 2009			792			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		56		4			104842		European Al Association 2009			4880			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		693		05		gt		5000		European Al Association 2009		gt		713			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		120		788		05		gt		5000		European Al Association 2009		gt		590			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		120		676		2		gt		15000		European Al Association 2009		gt		1212			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		771		2		gt		15000		European Al Association 2009		gt		810			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		66		4		gt		15000		European Al Association 2009		gt		1014			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		76		4		gt		15000		European Al Association 2009		gt		555			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			11971		European Al Association 2010			270			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			27478		European Al Association 2010			619			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			11998		European Al Association 2010			270			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		607		05			92495		European Al Association 2010			188			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		609		05			31337		European Al Association 2010			604			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		61		05			33235		European Al Association 2010			624			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		708		05		gt		8864		European Al Association 2010		gt		253			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		779		05		gt		42783		European Al Association 2010		gt		693			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		753		05			13204		European Al Association 2010			24			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		600		601		05			46326		European Al Association 2010			380			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		600		599		05		gt		8590		European Al Association 2010		gt		737			509				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia reticulata		F M T		451		60		11			304		Shephard 1983			201			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia reticulata		F M T		40		55		11			362		Shephard 1983			5531			1054		733			Ceriodaphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake SuperiorDI Mix
	Cladoceran Daphnia magna		S U NR		453		774		11			3900		Biesinger and Christensen 1972			338			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Daphnia magna		S M T		220		76		16			38200		Kimball 1978			2030			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Well water
	Cladoceran Daphnia magna		S U T		451		725		11			2800		Shephard 1983			287			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Daphnia magna		S U T		168		599		05			5000		European Al Association 2009		gt		227			-				Daphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Daphnia magna		S U T		168		792		05			7950		European Al Association 2009			92			333		333			Daphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Ostracod Stenocypris major		R M T		1563		651		16			310196		Shuhaimi-Othman et al 2011a			1022			1022		1022			Stenocypris		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (Malaysia)
	Amphipod Crangonyx pseudogracilis		R U T		50		675		16			9190		Martin and Holdich 1986			1217			1217		1217			Crangonyx		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (UK)
	Amphipod Hyalella azteca		F M T		105		613		048		gt		5997		Wang et al 2016 Wang et al 2017		gt		2800		gt		2800		2800		gt		Hyalella		Invert		Author reported
	Stonefly Acroneuria sp		S M T		474		746		11		gt		22600		Call et al 1984		gt		2050		gt		2050		2050		gt		Acroneuria		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Midge Chironomus plumosus		S U T		80		70		16			30000		Fargasova 2001			2536			2536		2536			Chironomus		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (Slovakia)
	Midge Paratanytarsus dissimilis		S M T		1743		728		28		gt		77700		Lamb and Bailey 1981 1983		gt		6232		gt		6232		6232		gt		Paratanytarsus		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Liberty Lake Washington
	Rainbow trout Oncorhynchus mykiss		F M T		274		758		05		gt		9840		Gundersen et al 1994		gt		1256			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		452		762		05		gt		8070		Gundersen et al 1994		gt		1140			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		895		758		05		gt		8160		Gundersen et al 1994		gt		1489			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		1304		758		05		gt		8200		Gundersen et al 1994		gt		1676			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		232		825		05			6170		Gundersen et al 1994			260			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		350		826		05			6170		Gundersen et al 1994			333			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		836		829		05			7670		Gundersen et al 1994			701			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		1158		830		05			6930		Gundersen et al 1994			777			801		801			Oncorhynchus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Atlantic salmon Salmo salar		S U T		68		55		05			584		Hamilton and Haines 1995			4602			-				Salmo			Value is from 2007 FW Cu AWQC Appendix C recommendation		Soft recon
	Atlantic salmon Salmo salar		S U T		68		65		05			599		Hamilton and Haines 1995			531			1564		1564			Salmo		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Soft recon
	Brook trout Salvelinus fontinalis		S U T		40		56		16			6530		Tandjung 1982			6947			-				Salvelinus			Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Brook trout Salvelinus fontinalis		S U T		18		56		16			3400		Tandjung 1982			6209			-				Salvelinus			Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Brook trout Salvelinus fontinalis		S U T		2		56		16			370		Tandjung 1982			2988			5051		5051			Salvelinus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Green sunfish Lepomis cyanellus		S M T		474		755		11		gt		50000		Call et al 1984		gt		5255		gt		5255		5255		gt		Lepomis		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Rio Grande silvery minnow Hybognathus amarus		R M T		140		81		05		gt		59100		Buhl 2002		gt		8620		gt		8620		8620		gt		Hybognathus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon water
	Fathead minnow Pimephales promelas		S M T		474		761		11		gt		48200		Call et al 1984		gt		4707			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Fathead minnow Pimephales promelas		S M T		474		805		11		gt		49800		Call et al 1984		gt		2837			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Fathead minnow Pimephales promelas		R M T		26		78		05			1160		ENSR 1992c			104			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		46		76		05			8180		ENSR 1992c			1191			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		96		81		05			20300		ENSR 1992c			2398			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		194		81		05			44800		ENSR 1992c			7839			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		140		81		05		gt		59100		Buhl 2002		gt		8620			2224		2224			Pimephales		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon water
	Smallmouth bass Micropterus dolomieui		S M T		1245		505		16			130		Kane and Rabeni 1987			859			859		859			Micropterus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Green tree frog Hyla cinerea		R M T		455		549		05		gt		4052		Jung and Jagoe 1995		gt		4377		gt		4377		4377		gt		Hyla		Amphibian		Value is from 2007 FW Cu AWQC Appendix C recommendation		artificial soft recon

	Normalization Chemistry
	pH		6					FAV		360
	Hardness		25					CMC		180
	DOC		1					CCC		99
	Acute Ranked GMAV											Chronic Ranked GMCV
	Rank			GMAV		Genus								Rank			GMCV		Genus
	18		gt		8620		Hybognathus		Fish							12		gt		2131		Rana		Amphib - Other Data
	17		gt		6232		Paratanytarsus		Invert							11			1710		Aeolosoma		Invert
	16		gt		5255		Lepomis		Fish							10			591		Chironomus		Invert
	15			5051		Salvelinus		Fish							9			544		Pimephales		Fish
	14		gt		4377		Hyla		Amphibian							8			488		Lymnaea		Mollusk
	13			3546		Physa		Mollusk							7			255		Brachionus		Invert
	12		gt		2983		Lampsilis		Mollusk							6			241		Danio		Fish
	11		gt		2800		Hyalella		Invert							5			171		Hyalella		Invert
	10			2536		Chironomus		Invert							4			129		Salvelinus		Fish
	9			2224		Pimephales		Fish							3			118		Ceriodaphnia		Invert
	8		gt		2050		Acroneuria		Invert							2			111		Salmo		Fish
	7			1564		Salmo		Fish							1			104		Lampsilis		Mollusk
	6			1217		Crangonyx		Invert
	5			1022		Stenocypris		Invert
	4			859		Micropterus		Fish
	3			801		Oncorhynchus		Fish
	2			733		Ceriodaphnia		Invert
	1			333		Daphnia		Invert
		N		Rank		Genus		GMAV		ln(GMAV)		ln(GMAV)2		P=R(N+1)		sqrt(P)				N		Rank		Genus		GMCV		ln(GMCV)		ln(GMCV)2		P=R(N+1)		sqrt(P)
		18		4		Micropterus		859		676		4564		0211		0459				12		4		Salvelinus		129		486		2364		0308		0555
			3		Oncorhynchus		801		669		4469		0158		0397					3		Ceriodaphnia		118		477		2277		0231		0480
			2		Ceriodaphnia		733		660		4352		0105		0324					2		Salmo		111		471		2220		0154		0392
			1		Daphnia		333		581		3374		0053		0229					1		Lampsilis		104		465		2159		0077		0277
			Sum				2585		1676		0526		141					Sum				1899		902		0769		170
			S2 =		1987										S2 =		059
			L =		4890										L =		4420
			A =		5887										A =		4592
			FAV =		360										FCV =		987
			CMC =		180

				All concentrations reported are microgL total Aluminum
				lt-----Click Button after entering data
	ENTER DATA HERE									ERRORNUM		ACUTE														CHRONIC
										Rank 1				Rank 2				Rank 3				Rank 4					Rank 1				Rank 2				Rank 3				Rank 4
	SiteName		DOC(mgL)		Hardness(mgL as CaCO3)		pH			FAV		CMC		CCC			Genus		GMAV			Genus		GMAV			Genus		GMAV			Genus		GMAV				Genus		GMCV			Genus		GMCV			Genus		GMCV			Genus		GMCV
	Site 1		50		25		9		75428737034162		35229324666008		1800		770			Daphnia			33452		Ceriodaphnia			73576		Stenocypris			102576		Crangonyx			122230			Lampsilis			10463		Ceriodaphnia			11865		Hyalella			17200		Brachionus			25648
	Site 2		50		50		9		75428737034162		31204991509063		1600		680			Daphnia			29631		Ceriodaphnia			65171		Stenocypris			90859		Crangonyx			108267			Lampsilis			9268		Ceriodaphnia			10510		Hyalella			15236		Brachionus			22718
	Site 3		50		75		9		75428737034162		29067527928199		1500		640			Daphnia			27601		Ceriodaphnia			60707		Stenocypris			84635		Crangonyx			100851			Lampsilis			8633		Ceriodaphnia			9790		Hyalella			14192		Brachionus			21162
	Site 4		50		100		9		75428737034162		27640367912592		1400		600			Daphnia			26246		Ceriodaphnia			57726		Stenocypris			80480		Crangonyx			95899			Lampsilis			8209		Ceriodaphnia			9309		Hyalella			13495		Brachionus			20123
	Site 5		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 6		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 7		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 8		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 9		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 10		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 11		50		25		85		75428737034162		63245275664384		3200		1400			Daphnia			60055		Ceriodaphnia			132086		Stenocypris			184149		Crangonyx			219432			Lampsilis			18783		Ceriodaphnia			21301		Hyalella			30879		Brachionus			46045
	Site 12		50		50		85		75428737034162		61388044609926		3100		1300			Daphnia			58291		Ceriodaphnia			128208		Stenocypris			178742		Crangonyx			212988			Lampsilis			18232		Ceriodaphnia			20676		Hyalella			29972		Brachionus			44693
	Site 13		50		75		85		75428737034162		60327020429132		3000		1300			Daphnia			57284		Ceriodaphnia			125992		Stenocypris			175652		Crangonyx			209307			Lampsilis			17917		Ceriodaphnia			20318		Hyalella			29454		Brachionus			43920
	Site 14		50		100		85		75428737034162		59585352130146		3000		1300			Daphnia			56580		Ceriodaphnia			124443		Stenocypris			173493		Crangonyx			206734			Lampsilis			17696		Ceriodaphnia			20068		Hyalella			29092		Salmo			41264
	Site 15		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 16		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 17		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 18		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 19		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 20		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370

	Appendix C Acceptable Chronic Toxicity Data of Aluminum to Freshwater Aquatic Animals
									MLR equation
	Species		Hardness (mgL as CaCO3)		pH		DOC(mgL)		EC20 Endpoint			EC20(microgL)			Normalized Conc- EC20(microgL)			SMCV-EC20(microgL)		GMCV-EC20(microgL)			Genus			DOC Notes		Dilution water
	Oligochaete Aeolosoma sp		48		6		025		Reproduction (population size)			1235			17098			17098		17098			Aeolosoma		Invert		Author reported half the detection limit
	Rotifer Brachionus calyciflorus		100		62		025		Reproduction (population size)			431			2555			2555		2555			Brachionus		Invert		Author reported half the detection limit
	Great pond snail Lymnaea stagnalis		117		61		025		Biomass			746			4877			4877		4877			Lymnaea		Mollusk		Author reported half the detection limit
	Fatmucket Lampsilis siliquoidea		1055		604		040		Biomass			169			1042			1042		1042			Lampsilis		Mollusk		Author reported
	Cladoceran Ceriodaphnia dubia		50		715		11		Reproduction - youngstarting adult			1780			1898						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		26		73		05		Reproduction - youngfemale			1557			2718						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		46		75		05		Reproduction - youngfemale			8087			1102						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		96		79		05		Reproduction - youngfemale			6519			796						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		194		81		05		Reproduction - youngfemale			6836			888						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		255		703		05		Reproduction (youngfemale)			250			563						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		122		713		05		Reproduction (youngfemale)			860			1061						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		25		798		05		Reproduction (youngfemale)			700			1006						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		61		803		05		Reproduction (youngfemale)			1010			1363						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		122		810		05		Reproduction (youngfemale)			870			1164						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		255		633		05		Reproduction (youngfemale)			260			1758						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		121		636		05		Reproduction (youngfemale)			390			1101			1182		1182			Ceriodaphnia		Invert		Author reported
	Amphipod Hyalella azteca		95		61		051		Biomass (28 d)			1993			1014						Hyalella			Author reported
	Amphipod Hyalella azteca		106		604		033		Biomass (28 d)			425			2895			1713		1713			Hyalella		Invert		Author reported
	Midge Chironomus riparius		118		558		18		Adult midge emergence			2955			1192						Chironomus			Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Midge Chironomus riparius		119		505		18		Adult midge emergence			8442			22578						Chironomus			Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Midge Chironomus riparius		91		66		051		Reproduction ( of eggscase)			3387			7664			5908		5908			Chironomus		Invert		Author reported
	Atlantic salmon Salmo salar		127		57		18		Biomass			6156			5085			5085		5085			Salmo		Fish		Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Brook trout Salvelinus fontinalis		123		655		19		Biomass			1644			270						Salvelinus			Author reported		wellRO mix
	Brook trout Salvelinus fontinalis		128		565		18		Biomass			1435			1295			5914		5914			Salvelinus		Fish		Author reported		wellRO mix
	Fathead minnow Pimephales promelas		220		770		16		Biomass			6194			3569						Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		well water
	Fathead minnow Pimephales promelas		96		620		025		Fry survival			4286			1734			2488		2488			Pimephales		Fish		Author reported half the detection limit
	Zebrafish Danio rerio		83		61		025		Biomass			2344			1102			1102		1102			Danio		Fish		Author reported half the detection limit
	Wood frog Rana sylvatica		115		469		16		MATC-survival		gt		2000		gt		9746		gt		9746		9746		gt		Rana		Amphib - Other Data		Value is from 2007 FW Cu AWQC Appendix C recommendation		aged tap water

	Appendix A Acceptable Acute Toxicity Data of Aluminum to Freshwater Aquatic Animals
	Species		Methoda		Hardness (mgL as CaCO3)		pH		DOC(mgL)			LC50 or EC50 Conc (microgL)		Reference			Normalized Conc - LC50(microgL)			Species Mean Acute Value(microgL)		Genus Mean Acute Value(microgL)			Genus			DOC Note		Dilution water
	Snail Physa sp		S M T		474		659		11		gt		23400		Call 1984 Call et al 1984		gt		48425			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		755		11			30600		Call 1984 Call et al 1984			26986			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		817		11		gt		24700		Call 1984 Call et al 1984		gt		24040			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		746		11			55500		Call 1984 Call et al 1984			50338			35462		35462			Physa		Mollusk		Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Fatmucket Lampsilis siliquoidea		F M T		1060		612		048		gt		6302		Wang et al 2016 Wang et al 2017		gt		29834		gt		29834		29834		gt		Lampsilis		Mollusk		Author reported
	Cladoceran Ceriodaphnia dubia		S M A		500		742		11			1900		McCauley et al 1986			1728			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		S M A		505		786		11			1500		McCauley et al 1986			1294			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		UW-S Lake Superior lab water
	Cladoceran Ceriodaphnia dubia		S M A		500		813		11			2560		McCauley et al 1986			2431			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		R M T		26		75		05			720		ENSR 1992d			1113			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		R M T		46		76		05			1880		ENSR 1992d			2495			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		R M T		96		78		05			2450		ENSR 1992d			2894			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		S U NR		90		715		05			3727		Fort and Stover 1995			5002			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U NR		90		715		05			5673		Fort and Stover 1995			7613			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U NR		89		76		05			2880		Soucek et al 2001			3362			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		601		05			7112		European Al Association 2009			1691			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		605		2			6865		European Al Association 2009			7088			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		609		4			15581		European Al Association 2009			10072			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		601		05			681		European Al Association 2009			1620			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		603		05			1630		European Al Association 2009			3674			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		597		05			1785		European Al Association 2009			4732			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		592		05			1410		European Al Association 2009			4295			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		699		05		gt		1300		European Al Association 2009		gt		4189			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		785		05		gt		5000		European Al Association 2009		gt		7960			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		68		2		gt		10000		European Al Association 2009		gt		20755			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		782		2		gt		15000		European Al Association 2009		gt		11626			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		677		4		gt		10000		European Al Association 2009		gt		15161			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		766		4		gt		15000		European Al Association 2009		gt		8608			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		79		05		gt		2000		European Al Association 2009		gt		3150			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		789		05		gt		2000		European Al Association 2009		gt		3156			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		604		05			1108		European Al Association 2009			850			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		598		2			11371		European Al Association 2009			4817			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		573		4			80467		European Al Association 2009			43570			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		671		05		gt		10000		European Al Association 2009		gt		23786			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		783		05		gt		5000		European Al Association 2009		gt		6344			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		679		2		gt		10000		European Al Association 2009		gt		10394			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		767		2		gt		15000		European Al Association 2009		gt		9082			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		668		4		gt		15000		European Al Association 2009		gt		12461			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		762		4		gt		15000		European Al Association 2009		gt		6332			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		606		2			33868		European Al Association 2009			7920			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		56		4			104842		European Al Association 2009			48800			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		693		05		gt		5000		European Al Association 2009		gt		7126			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		120		788		05		gt		5000		European Al Association 2009		gt		5898			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		120		676		2		gt		15000		European Al Association 2009		gt		12117			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		771		2		gt		15000		European Al Association 2009		gt		8096			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		66		4		gt		15000		European Al Association 2009		gt		10139			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		76		4		gt		15000		European Al Association 2009		gt		5545			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			11971		European Al Association 2010			2699			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			27478		European Al Association 2010			6194			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			11998		European Al Association 2010			2705			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		607		05			92495		European Al Association 2010			1876			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		609		05			31337		European Al Association 2010			6035			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		61		05			33235		European Al Association 2010			6238			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		708		05		gt		8864		European Al Association 2010		gt		2534			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		779		05		gt		42783		European Al Association 2010		gt		6930			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		753		05			13204		European Al Association 2010			244			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		600		601		05			46326		European Al Association 2010			3798			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		600		599		05		gt		8590		European Al Association 2010		gt		7366			5094				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia reticulata		F M T		451		60		11			304		Shephard 1983			2009			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia reticulata		F M T		40		55		11			362		Shephard 1983			55307			10542		7328			Ceriodaphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake SuperiorDI Mix
	Cladoceran Daphnia magna		S U NR		453		774		11			3900		Biesinger and Christensen 1972			3382			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Daphnia magna		S M T		220		76		16			38200		Kimball 1978			20303			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Well water
	Cladoceran Daphnia magna		S U T		451		725		11			2800		Shephard 1983			2868			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Daphnia magna		S U T		168		599		05			5000		European Al Association 2009		gt		2265			-				Daphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Daphnia magna		S U T		168		792		05			7950		European Al Association 2009			920			3332		3332			Daphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Ostracod Stenocypris major		R M T		1563		651		16			310196		Shuhaimi-Othman et al 2011a			10216			10216		10216			Stenocypris		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (Malaysia)
	Amphipod Crangonyx pseudogracilis		R U T		50		675		16			9190		Martin and Holdich 1986			12174			12174		12174			Crangonyx		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (UK)
	Amphipod Hyalella azteca		F M T		105		613		048		gt		5997		Wang et al 2016 Wang et al 2017		gt		28002		gt		28002		28002		gt		Hyalella		Invert		Author reported
	Stonefly Acroneuria sp		S M T		474		746		11		gt		22600		Call et al 1984		gt		20498		gt		20498		20498		gt		Acroneuria		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Midge Chironomus plumosus		S U T		80		70		16			30000		Fargasova 2001			25361			25361		25361			Chironomus		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (Slovakia)
	Midge Paratanytarsus dissimilis		S M T		1743		728		28		gt		77700		Lamb and Bailey 1981 1983		gt		62318		gt		62318		62318		gt		Paratanytarsus		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Liberty Lake Washington
	Rainbow trout Oncorhynchus mykiss		F M T		274		758		05		gt		9840		Gundersen et al 1994		gt		5745			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		452		762		05		gt		8070		Gundersen et al 1994		gt		5212			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		895		758		05		gt		8160		Gundersen et al 1994		gt		6807			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		1304		758		05		gt		8200		Gundersen et al 1994		gt		7662			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		232		825		05			6170		Gundersen et al 1994			1190			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		350		826		05			6170		Gundersen et al 1994			1523			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		836		829		05			7670		Gundersen et al 1994			3205			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		1158		830		05			6930		Gundersen et al 1994			3554			3661		3661			Oncorhynchus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Atlantic salmon Salmo salar		S U T		68		55		05			584		Hamilton and Haines 1995			21042			-				Salmo			Value is from 2007 FW Cu AWQC Appendix C recommendation		Soft recon
	Atlantic salmon Salmo salar		S U T		68		65		05			599		Hamilton and Haines 1995			2430			7151		7151			Salmo		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Soft recon
	Brook trout Salvelinus fontinalis		S U T		40		56		16			6530		Tandjung 1982			31767			-				Salvelinus			Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Brook trout Salvelinus fontinalis		S U T		18		56		16			3400		Tandjung 1982			28391			-				Salvelinus			Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Brook trout Salvelinus fontinalis		S U T		2		56		16			370		Tandjung 1982			13663			23097		23097			Salvelinus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Green sunfish Lepomis cyanellus		S M T		474		755		11		gt		50000		Call et al 1984		gt		24028		gt		24028		24028		gt		Lepomis		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Rio Grande silvery minnow Hybognathus amarus		R M T		140		81		05		gt		59100		Buhl 2002		gt		39414		gt		39414		39414		gt		Hybognathus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon water
	Fathead minnow Pimephales promelas		S M T		474		761		11		gt		48200		Call et al 1984		gt		21522			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Fathead minnow Pimephales promelas		S M T		474		805		11		gt		49800		Call et al 1984		gt		12972			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Fathead minnow Pimephales promelas		R M T		26		78		05			1160		ENSR 1992c			477			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		46		76		05			8180		ENSR 1992c			5446			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		96		81		05			20300		ENSR 1992c			10966			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		194		81		05			44800		ENSR 1992c			35847			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		140		81		05		gt		59100		Buhl 2002		gt		39414			10168		10168			Pimephales		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon water
	Smallmouth bass Micropterus dolomieui		S M T		1245		505		16			130		Kane and Rabeni 1987			3929			3929		3929			Micropterus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Green tree frog Hyla cinerea		R M T		455		549		05		gt		4052		Jung and Jagoe 1995		gt		20016		gt		20016		20016		gt		Hyla		Amphibian		Value is from 2007 FW Cu AWQC Appendix C recommendation		artificial soft recon

	Normalization Chemistry
	pH		7					FAV		2741
	Hardness		100					CMC		1400
	DOC		1					CCC		390
	Acute Ranked GMAV											Chronic Ranked GMCV
	Rank			GMAV		Genus								Rank			GMCV		Genus
	18		gt		62318		Paratanytarsus		Invert							12			17098		Aeolosoma		Invert
	17		gt		39414		Hybognathus		Fish							11		gt		9746		Rana		Amphib - Other Data
	16			35462		Physa		Mollusk							10			5908		Chironomus		Invert
	15		gt		29834		Lampsilis		Mollusk							9			4877		Lymnaea		Mollusk
	14		gt		28002		Hyalella		Invert							8			2555		Brachionus		Invert
	13			25361		Chironomus		Invert							7			2488		Pimephales		Fish
	12		gt		24028		Lepomis		Fish							6			1713		Hyalella		Invert
	11			23097		Salvelinus		Fish							5			1182		Ceriodaphnia		Invert
	10		gt		20498		Acroneuria		Invert							4			1102		Danio		Fish
	9		gt		20016		Hyla		Amphibian							3			1042		Lampsilis		Mollusk
	8			12174		Crangonyx		Invert							2			591		Salvelinus		Fish
	7			10216		Stenocypris		Invert							1			509		Salmo		Fish
	6			10168		Pimephales		Fish
	5			7328		Ceriodaphnia		Invert
	4			7151		Salmo		Fish
	3			3929		Micropterus		Fish
	2			3661		Oncorhynchus		Fish
	1			3332		Daphnia		Invert
		N		Rank		Genus		GMAV		ln(GMAV)		ln(GMAV)2		P=R(N+1)		sqrt(P)				N		Rank		Genus		GMCV		ln(GMCV)		ln(GMCV)2		P=R(N+1)		sqrt(P)
		18		4		Salmo		7151		887		7876		0211		0459				12		4		Danio		1102		700		4907		0308		0555
			3		Micropterus		3929		828		6849		0158		0397					3		Lampsilis		1042		695		4829		0231		0480
			2		Oncorhynchus		3661		821		6733		0105		0324					2		Salvelinus		591		638		4074		0154		0392
			1		Daphnia		3332		811		6579		0053		0229					1		Salmo		509		623		3883		0077		0277
			Sum				3347		2804		0526		141					Sum				2657		1769		0769		170
			S2 =		1223										S2 =		1080
			L =		7134										L =		5242
			A =		7916										A =		5976
			FAV =		2741										FCV =		3940
			CMC =		1371

				All concentrations reported are microgL total Aluminum
				lt-----Click Button after entering data
	ENTER DATA HERE									ERRORNUM		ACUTE														CHRONIC
										Rank 1				Rank 2				Rank 3				Rank 4					Rank 1				Rank 2				Rank 3				Rank 4
	SiteName		DOC(mgL)		Hardness(mgL as CaCO3)		pH			FAV		CMC		CCC			Genus		GMAV			Genus		GMAV			Genus		GMAV			Genus		GMAV				Genus		GMCV			Genus		GMCV			Genus		GMCV			Genus		GMCV
	Site 1		50		25		9		75428737034162		35229324666008		1800		770			Daphnia			33452		Ceriodaphnia			73576		Stenocypris			102576		Crangonyx			122230			Lampsilis			10463		Ceriodaphnia			11865		Hyalella			17200		Brachionus			25648
	Site 2		50		50		9		75428737034162		31204991509063		1600		680			Daphnia			29631		Ceriodaphnia			65171		Stenocypris			90859		Crangonyx			108267			Lampsilis			9268		Ceriodaphnia			10510		Hyalella			15236		Brachionus			22718
	Site 3		50		75		9		75428737034162		29067527928199		1500		640			Daphnia			27601		Ceriodaphnia			60707		Stenocypris			84635		Crangonyx			100851			Lampsilis			8633		Ceriodaphnia			9790		Hyalella			14192		Brachionus			21162
	Site 4		50		100		9		75428737034162		27640367912592		1400		600			Daphnia			26246		Ceriodaphnia			57726		Stenocypris			80480		Crangonyx			95899			Lampsilis			8209		Ceriodaphnia			9309		Hyalella			13495		Brachionus			20123
	Site 5		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 6		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 7		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 8		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 9		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 10		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 11		50		25		85		75428737034162		63245275664384		3200		1400			Daphnia			60055		Ceriodaphnia			132086		Stenocypris			184149		Crangonyx			219432			Lampsilis			18783		Ceriodaphnia			21301		Hyalella			30879		Brachionus			46045
	Site 12		50		50		85		75428737034162		61388044609926		3100		1300			Daphnia			58291		Ceriodaphnia			128208		Stenocypris			178742		Crangonyx			212988			Lampsilis			18232		Ceriodaphnia			20676		Hyalella			29972		Brachionus			44693
	Site 13		50		75		85		75428737034162		60327020429132		3000		1300			Daphnia			57284		Ceriodaphnia			125992		Stenocypris			175652		Crangonyx			209307			Lampsilis			17917		Ceriodaphnia			20318		Hyalella			29454		Brachionus			43920
	Site 14		50		100		85		75428737034162		59585352130146		3000		1300			Daphnia			56580		Ceriodaphnia			124443		Stenocypris			173493		Crangonyx			206734			Lampsilis			17696		Ceriodaphnia			20068		Hyalella			29092		Salmo			41264
	Site 15		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 16		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 17		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 18		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 19		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 20		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370

Aluminum Standardsbull Aluminum Standards

ndash About 20 states have numeric criteria for aluminum for aquatic life

bull Most are based on EPArsquos 1988 values and two are hardness-based equations (CO and NM)

bull There are numerous site-specific criteria that involve the Biotic Ligand Model (BLM) or a Water Effects Ratio (WER) -adjusted 1988 aluminum criteria

ndash Aluminum is not a priority pollutant

7

Criteria Development Processbull All EPA Aquatic Life Criteria for Toxics undergo rigorous

scientific development and review processbull Develop criteria document draftbull Intra-agency peer review process bull Independent external peer reviewbull Revisions based on external peer review

Intra-agency review of draft criteria documentbull Release to the public in draft form to obtain scientific views

Revisions considering public comments Intra-agency review of final criteria document

bull Publication of final criteria document bull States may then consider recommended criteria in

development and adoption of state water quality standards8

Framework for Updated Draft Aluminum Criteria

I Screen toxicological studies for high

quality data

II Consider water conditions effects on

aluminum bioavailability

III Normalize toxicity data in species

sensitivity distribution to a set of water quality conditions

IV Construct sensitivity distribution to determine criteria

at set conditions

9

I Screen toxicological studies for high quality data

bull First step in all criteria developmentndash Data are evaluated based on the data quality objectives

outlined in the 1985 Guidelines methodology and other toxicity testing recommendations (ie EPA ASTM)

ndash High quality data are used quantitatively to develop criteria magnitudes

ndash Lower quality data are used qualitatively if appropriatendash Lowest quality data are considered unacceptable and not

usedbull Data are grouped into acute and chronic exposures

10

Aluminum Toxicity Databull The updated freshwater aluminum criteria meets the

acute and chronic minimum data requirements outlined in EPArsquos 1985 Guidelines

InvertebrateSpecies

Fish Species

AmphibianSpecies

Total Genera

Acute1988 AWQC 8 7 - 142017 Draft AWQC 11 8 1 18

Chronic1988 AWQC 2 1 - 32017 Draft AWQC 7 4 - 11

11 1988 AWQC Chronic CCC based on two studies for the striped

bass and brook trout

Aluminum Toxicity Data Freshwater mussels have been

suggested to be potentially sensitive to aluminum

EPA worked collaboratively with USGSrsquos Columbia Environmental Research Center (CERC) to have acute and chronic aluminum toxicity tests conducted with a unionid mussel (fatmucket Lampsilis siliquoidea) in water-only exposures to obtain additional data on invertebrates New data on amphipod (Hyalella azteca)

were collected to confirm laboratory conditions

12

Aluminum Toxicity Data

13

bull New data and models examinedndash Data submitted to EU REACH program

bull New freshwater aluminum toxicity data generated by Oregon State University

bull 2016 BLM model provided by the European Aluminum Association

ndash Saltwater data provided by Australia bull Insufficient data to develop estuarinemarine acute or chronic

criteria at this timendash Multiple Linear Regression (MLR) model developed by

DeForest et al 2017bull DeForest DK KV Brix LM Tear and WJ Adams 2017 Multiple Linear Regression (MLR) models for predicting chronic aluminum

toxicity to freshwater aquatic organisms and developing water quality guidelines Environ Toxicol Chem (DOI101002etc3922)

14

Minimum Dataset For Freshwater Criteria Derivation

SALMONID SECONDFISHFAMILY

CHORDATA

PLANKTONICCRUSTACEAN

BENTHIC CRUSTACEAN

INSECT ROTIFERA ANNELIDA MOLLUSCA

OTHERINSECT ORMOLLUSCA

EstuarineMarine Minimum Data Requirements

15

Family Minimum Data Requirement EstuarineMarine

Acute(Phylum Family Genus)

Chronic(Phylum Family Genus)

Family in the phylum Chordata - -

Family in the phylum Chordata - -

Either the Mysidae or Penaeidae family - -

Family in a phylum other than Arthropoda or Chordata Mollusca Ostreidae Crassostrea -

Family in a phylum other than Chordata Annelida Nereididae Neanthes -

Family in a phylum other than Chordata Annelida Capitellidae Capitella -

Family in a phylum other than Chordata Annelida Ctenodrilidae Ctenodrilus -

Any other family Arthropoda Ameiridae Nitokra -

II Evaluate water quality conditions to determine applicability

bull Criteria are often based on one or more water quality conditions (eg hardness) that affect toxicity of the material to test organisms

bull Aluminum toxicity is highly dependent on the pH of the test waterndash The pH of test waters affects the amount and form

of aluminum that is bioavailable to test speciesndash Hardness and DOC (Dissolved Organic Carbon)

also influence the amount of aluminum that is bioavailable

16

Multiple Bioavailability Modeling Approaches Underwent External Peer Review

17

bull Biotic Ligand Modelsbull Full aluminum BLM (2016 model version) ndash Temperature pH

Dissolved organic carbon (DOC) Major cations (calcium [Ca] magnesium [Mg] sodium [Na] and potassium [K]) Major anions (sulfate [SO4] and chloride [Cl]) Alkalinity and Sulfide

bull Simplified aluminum BLM - pH hardness DOC temperature

bull Multilinear Regression Equationsbull pH and Hardness

Accounting for Effects of Water Chemistry on Bioavailability and Toxicity

bull Water quality criteria can be modified quantitatively when enough data are available to demonstrate that those conditions similarly affect the results with a variety of speciesndash High quality data in the draft aluminum document demonstrated

that pH DOC and hardness affected a variety of species in a similar way when exposed to aluminum concentrations

bull Recent publications by Cardwell et al (2017) and Gensemer et al (2017) summarized short-term aluminum chronic toxicity data across a range of DOC pH and hardness values for three species

18

Multiple Linear Regression (MLR) Models to Account for Effects of Water Chemistry

bull MLR models were developed for each species (DeForest et al 2017)ndash 119862119862 119889119889119906119906119887119887119894119894119886119886 11986411986411986211986220=119890119890[minus41026+[0525timesln(119863119863119874119874119862119862)]+[2201timesln(ℎ119886119886119903119903119889119889)]+(11282times119901119901119867119867)minus(0633times1199011199011198671198672)minus[0264times119901119901119867119867ln(ℎ119886119886119903119903119889119889)]]

ndash 119875119875 119901119901119903119903119900119900119898119898119890119890119897119897119886119886119904119904 11986411986411986211986220=119890119890[minus14029+[0503timesln(119863119863119874119874119862119862)]+[3443timesln(ℎ119886119886119903119903119889119889)]+(3131times119901119901119867119867)minus[0494times119901119901119867119867ln(ℎ119886119886119903119903119889119889)]]

bull These models were applied to both the acute and chronic aluminum toxicity datandash The C dubia model was applied to invertebrate datandash The P promelas model was applied to vertebrate data

19

III Normalize toxicity data in species sensitivity distribution to a set of water

quality conditionsbull Just like single parameter hardness models the MLR

models were used to normalize the aluminum toxicity data to a single set of water quality conditionsndash As an illustration for one set of calculations the draft

aluminum AWQC set these conditions to pH 7 hardness of 100 mgL and DOC of 1 mgL

bull This allows for comparisons to be made between species based on their sensitivity to aluminumndash Apples to apples

20

IV Construct sensitivity distribution to determine criteria at set conditions

21

Figure 8 Ranked Summary of Total Aluminum Genus Mean Acute Values (GMAVs) - Freshwater at pH 7 Hardness of 100 mgL and DOC of 1 mgL

IV Construct sensitivity distribution to determine criteria at set conditions

22

Figure 10 Ranked Summary of Total Aluminum Genus Mean Chronic Values (GMCVs) ndash Freshwater Supplemented with Other Data to Fulfill Missing MDRs at pH 7 Hardness of 100 mgL and DOC of 1 mgL

Aluminum Criteria Calculatorbull The relative ranking of genera in the sensitivity

distribution to which the toxicity data are normalized will change based on the set of water chemistry conditions (ie pH hardness and DOC)

bull To make calculating aluminum criteria easier EPA developed an Aluminum Criteria Calculatorndash Automated so that user only needs to enter water chemistry

conditions of interestndash Allows quick calculations for a variety of conditionsndash Limits calculations to the recommended conditions for which

the MLR models were developed were specified for hardness and DOC

bull No extrapolation beyond hardness and DOC empirical data bounds

23

Aluminum Criteria Calculator SD Example

24

pH=7 Hardness=100 DOC=1 pH=6 Hardness=25 DOC=1

Chronic Ranked GMCVRank GMCV Genus

12 17098 Aeolosoma Invert11 gt 9746 Rana Amphib - Other Data10 5908 Chironomus Invert

9 4877 Lymnaea Mollusk8 2555 Brachionus Invert7 2488 Pimephales Fish6 1713 Hyalella Invert5 1182 Ceriodaphnia Invert4 1102 Danio Fish3 1042 Lampsilis Mollusk2 591 Salvelinus Fish1 509 Salmo Fish

FCV = 3940 microgL total aluminum

Chronic Ranked GMCVRank GMCV Genus

12 gt 2131 Rana Amphib - Other Data11 1710 Aeolosoma Invert10 591 Chironomus Invert

9 544 Pimephales Fish8 488 Lymnaea Mollusk7 255 Brachionus Invert6 241 Danio Fish5 171 Hyalella Invert4 129 Salvelinus Fish3 118 Ceriodaphnia Invert2 111 Salmo Fish1 104 Lampsilis Mollusk

FCV = 987 microgL total aluminum

Multiple Scenarios

Run Scenarios

Summary Sheet

Acute Dataset

Chronic Dataset

Multiple Scenarios

Run Scenarios

Summary Sheet

Acute Dataset

Chronic Dataset

Boundaries of the Criteria Outputsbull MLR models were developed using data that

encompassndash DOC range of 008-5 mgLndash Hardness range of 98-127 mgL (as CaCO3)ndash pH range of 6-81

bull MLR criteria outputs are bounded atndash Maximum of 150 mgL total hardness (as CaCO3)

ndash DOC of 50 mgLndash pH range of the model is 50 to 90

25

Aluminum Criteria Look-Up Tables

26

bull EPA also provided criteria look-up tables in Appendix K

Har

dnes

s

CCC(microgL total aluminum)

(DOC=05 mgL)

pH 50 pH 55 pH 60 pH 65 pH 70 pH 75 pH 80 pH 85 pH 90

25 23 a 14 b 69 c 160 f 350 f 620 i 540 b 410 a 230 a

50 43 a 24 b 100 d 170 f 310 f 630 f 670 j 400 a 200 a

75 61 a 33 b 130 e 180 f 290 f 530 f 730 i 390 a 190 a

100 78 a 41 b 150 e 190 f 280 g 460 f 770 c 390 a 180 a

150 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

200 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

250 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

300 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

350 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

400 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

Aluminum Criteria Look-Up Tables

27

bull EPA also provided criteria look-up tables in Appendix K

Har

dnes

s

CCC(microgL total aluminum)

(DOC=10 mgL)

pH 50 pH 55 pH 60 pH 65 pH 70 pH 75 pH 80 pH 85 pH 90

25 33 a 21 b 99 c 220 f 490 f 900 i 780 b 590 a 330 a

50 61 a 35 b 150 e 250 f 440 f 890 f 960 j 580 a 290 a

75 88 a 47 b 180 e 260 f 410 f 740 f 1100 i 570 a 270 a

100 11 a 59 b 210 e 270 f 390 g 650 f 1100 c 560 b 260 a

150 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

200 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

250 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

300 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

350 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

400 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

Aluminum Criteria Look-Up Tables

28

bull EPA also provided criteria look-up tables in Appendix K

Har

dnes

s

CCC(microgL total aluminum)

(DOC=25 mgL)

pH 50 pH 55 pH 60 pH 65 pH 70 pH 75 pH 80 pH 85 pH 90

25 54 a 34 b 160 c 350 f 770 f 1500 i 1300 b 960 a 540 a

50 99 a 57 b 230 e 390 f 690 f 1400 f 1600 j 930 a 470 a

75 14 a 77 b 290 e 410 f 640 f 1200 f 1700 i 920 a 440 a

100 18 a 96 b 330 e 420 f 620 g 1000 f 1800 d 920 b 420 a

150 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

200 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

250 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

300 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

350 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

400 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

Aluminum Criteria Look-Up Tables

29

bull EPA also provided criteria look-up tables in Appendix K

Har

dnes

s

CCC(microgL total aluminum)

(DOC=50 mgL)

pH 50 pH 55 pH 60 pH 65 pH 70 pH 75 pH 80 pH 85 pH 90

25 77 a 49 b 230 c 500 f 1100 f 2100 c 1900 b 1400 a 770 a

50 14 a 82 b 330 e 540 f 970 f 2000 f 2200 j 1300 a 680 a

75 20 a 110 b 410 e 570 f 900 f 1600 f 2500 i 1300 a 640 a

100 26 a 140 b 460 f 590 f 880 g 1400 f 2500 d 1300 b 600 a

150 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

200 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

250 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

300 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

350 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

400 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

Status

30

bull Draft Document Released to the Federal Register on 72817ndash Public comment period of 60 days ndash Due Date for Comments is 92617

bull Revisions will be made considering public commentsbull Subsequent EPA intra-agency review of final criteria

document will then be conducted bull Publication of final criteria document planned for Late Fall

2017

Federal Register Webpagehttpswwwfederalregistergovdocuments201

707282017-15968request-for-scientific-views-draft-updated-aquatic-life-ambient-water-quality-criteria-for-aluminum

Aluminum Criteria Webpagehttpswwwepagovwqc2017-draft-aquatic-life-

criteria-aluminum-freshwater-documents

31

	Appendix C Acceptable Chronic Toxicity Data of Aluminum to Freshwater Aquatic Animals
									MLR equation
	Species		Hardness (mgL as CaCO3)		pH		DOC(mgL)		EC20 Endpoint			EC20(microgL)			Normalized Conc- EC20(microgL)			SMCV-EC20(microgL)		GMCV-EC20(microgL)			Genus			DOC Notes		Dilution water
	Oligochaete Aeolosoma sp		48		6		025		Reproduction (population size)			1235			1710			1710		1710			Aeolosoma		Invert		Author reported half the detection limit
	Rotifer Brachionus calyciflorus		100		62		025		Reproduction (population size)			431			255			255		255			Brachionus		Invert		Author reported half the detection limit
	Great pond snail Lymnaea stagnalis		117		61		025		Biomass			746			488			488		488			Lymnaea		Mollusk		Author reported half the detection limit
	Fatmucket Lampsilis siliquoidea		1055		604		040		Biomass			169			104			104		104			Lampsilis		Mollusk		Author reported
	Cladoceran Ceriodaphnia dubia		50		715		11		Reproduction - youngstarting adult			1780			190						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		26		73		05		Reproduction - youngfemale			1557			272						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		46		75		05		Reproduction - youngfemale			8087			110						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		96		79		05		Reproduction - youngfemale			6519			80						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		194		81		05		Reproduction - youngfemale			6836			89						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		255		703		05		Reproduction (youngfemale)			250			56						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		122		713		05		Reproduction (youngfemale)			860			106						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		25		798		05		Reproduction (youngfemale)			700			101						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		61		803		05		Reproduction (youngfemale)			1010			136						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		122		810		05		Reproduction (youngfemale)			870			116						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		255		633		05		Reproduction (youngfemale)			260			176						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		121		636		05		Reproduction (youngfemale)			390			110			118		118			Ceriodaphnia		Invert		Author reported
	Amphipod Hyalella azteca		95		61		051		Biomass (28 d)			1993			101						Hyalella			Author reported
	Amphipod Hyalella azteca		106		604		033		Biomass (28 d)			425			289			171		171			Hyalella		Invert		Author reported
	Midge Chironomus riparius		118		558		18		Adult midge emergence			2955			119						Chironomus			Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Midge Chironomus riparius		119		505		18		Adult midge emergence			8442			2258						Chironomus			Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Midge Chironomus riparius		91		66		051		Reproduction ( of eggscase)			3387			766			591		591			Chironomus		Invert		Author reported
	Atlantic salmon Salmo salar		127		57		18		Biomass			6156			1112			1112		1112			Salmo		Fish		Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Brook trout Salvelinus fontinalis		123		655		19		Biomass			1644			59						Salvelinus			Author reported		wellRO mix
	Brook trout Salvelinus fontinalis		128		565		18		Biomass			1435			283			1293		1293			Salvelinus		Fish		Author reported		wellRO mix
	Fathead minnow Pimephales promelas		220		770		16		Biomass			6194			781						Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		well water
	Fathead minnow Pimephales promelas		96		620		025		Fry survival			4286			379			544		544			Pimephales		Fish		Author reported half the detection limit
	Zebrafish Danio rerio		83		61		025		Biomass			2344			241			241		241			Danio		Fish		Author reported half the detection limit
	Wood frog Rana sylvatica		115		469		16		MATC-survival		gt		2000		gt		2131		gt		2131		2131		gt		Rana		Amphib - Other Data		Value is from 2007 FW Cu AWQC Appendix C recommendation		aged tap water

	Appendix A Acceptable Acute Toxicity Data of Aluminum to Freshwater Aquatic Animals
	Species		Methoda		Hardness (mgL as CaCO3)		pH		DOC(mgL)			LC50 or EC50 Conc (microgL)		Reference			Normalized Conc - LC50(microgL)			Species Mean Acute Value(microgL)		Genus Mean Acute Value(microgL)			Genus			DOC Note		Dilution water
	Snail Physa sp		S M T		474		659		11		gt		23400		Call 1984 Call et al 1984		gt		4843			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		755		11			30600		Call 1984 Call et al 1984			2699			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		817		11		gt		24700		Call 1984 Call et al 1984		gt		2404			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		746		11			55500		Call 1984 Call et al 1984			5034			3546		3546			Physa		Mollusk		Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Fatmucket Lampsilis siliquoidea		F M T		1060		612		048		gt		6302		Wang et al 2016 Wang et al 2017		gt		2983		gt		2983		2983		gt		Lampsilis		Mollusk		Author reported
	Cladoceran Ceriodaphnia dubia		S M A		500		742		11			1900		McCauley et al 1986			173			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		S M A		505		786		11			1500		McCauley et al 1986			129			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		UW-S Lake Superior lab water
	Cladoceran Ceriodaphnia dubia		S M A		500		813		11			2560		McCauley et al 1986			243			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		R M T		26		75		05			720		ENSR 1992d			111			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		R M T		46		76		05			1880		ENSR 1992d			250			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		R M T		96		78		05			2450		ENSR 1992d			289			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		S U NR		90		715		05			3727		Fort and Stover 1995			500			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U NR		90		715		05			5673		Fort and Stover 1995			761			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U NR		89		76		05			2880		Soucek et al 2001			336			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		601		05			7112		European Al Association 2009			169			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		605		2			6865		European Al Association 2009			709			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		609		4			15581		European Al Association 2009			1007			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		601		05			681		European Al Association 2009			162			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		603		05			1630		European Al Association 2009			367			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		597		05			1785		European Al Association 2009			473			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		592		05			1410		European Al Association 2009			429			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		699		05		gt		1300		European Al Association 2009		gt		419			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		785		05		gt		5000		European Al Association 2009		gt		796			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		68		2		gt		10000		European Al Association 2009		gt		2076			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		782		2		gt		15000		European Al Association 2009		gt		1163			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		677		4		gt		10000		European Al Association 2009		gt		1516			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		766		4		gt		15000		European Al Association 2009		gt		861			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		79		05		gt		2000		European Al Association 2009		gt		315			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		789		05		gt		2000		European Al Association 2009		gt		316			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		604		05			1108		European Al Association 2009			85			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		598		2			11371		European Al Association 2009			482			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		573		4			80467		European Al Association 2009			4357			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		671		05		gt		10000		European Al Association 2009		gt		2379			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		783		05		gt		5000		European Al Association 2009		gt		634			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		679		2		gt		10000		European Al Association 2009		gt		1039			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		767		2		gt		15000		European Al Association 2009		gt		908			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		668		4		gt		15000		European Al Association 2009		gt		1246			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		762		4		gt		15000		European Al Association 2009		gt		633			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		606		2			33868		European Al Association 2009			792			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		56		4			104842		European Al Association 2009			4880			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		693		05		gt		5000		European Al Association 2009		gt		713			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		120		788		05		gt		5000		European Al Association 2009		gt		590			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		120		676		2		gt		15000		European Al Association 2009		gt		1212			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		771		2		gt		15000		European Al Association 2009		gt		810			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		66		4		gt		15000		European Al Association 2009		gt		1014			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		76		4		gt		15000		European Al Association 2009		gt		555			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			11971		European Al Association 2010			270			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			27478		European Al Association 2010			619			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			11998		European Al Association 2010			270			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		607		05			92495		European Al Association 2010			188			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		609		05			31337		European Al Association 2010			604			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		61		05			33235		European Al Association 2010			624			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		708		05		gt		8864		European Al Association 2010		gt		253			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		779		05		gt		42783		European Al Association 2010		gt		693			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		753		05			13204		European Al Association 2010			24			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		600		601		05			46326		European Al Association 2010			380			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		600		599		05		gt		8590		European Al Association 2010		gt		737			509				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia reticulata		F M T		451		60		11			304		Shephard 1983			201			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia reticulata		F M T		40		55		11			362		Shephard 1983			5531			1054		733			Ceriodaphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake SuperiorDI Mix
	Cladoceran Daphnia magna		S U NR		453		774		11			3900		Biesinger and Christensen 1972			338			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Daphnia magna		S M T		220		76		16			38200		Kimball 1978			2030			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Well water
	Cladoceran Daphnia magna		S U T		451		725		11			2800		Shephard 1983			287			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Daphnia magna		S U T		168		599		05			5000		European Al Association 2009		gt		227			-				Daphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Daphnia magna		S U T		168		792		05			7950		European Al Association 2009			92			333		333			Daphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Ostracod Stenocypris major		R M T		1563		651		16			310196		Shuhaimi-Othman et al 2011a			1022			1022		1022			Stenocypris		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (Malaysia)
	Amphipod Crangonyx pseudogracilis		R U T		50		675		16			9190		Martin and Holdich 1986			1217			1217		1217			Crangonyx		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (UK)
	Amphipod Hyalella azteca		F M T		105		613		048		gt		5997		Wang et al 2016 Wang et al 2017		gt		2800		gt		2800		2800		gt		Hyalella		Invert		Author reported
	Stonefly Acroneuria sp		S M T		474		746		11		gt		22600		Call et al 1984		gt		2050		gt		2050		2050		gt		Acroneuria		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Midge Chironomus plumosus		S U T		80		70		16			30000		Fargasova 2001			2536			2536		2536			Chironomus		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (Slovakia)
	Midge Paratanytarsus dissimilis		S M T		1743		728		28		gt		77700		Lamb and Bailey 1981 1983		gt		6232		gt		6232		6232		gt		Paratanytarsus		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Liberty Lake Washington
	Rainbow trout Oncorhynchus mykiss		F M T		274		758		05		gt		9840		Gundersen et al 1994		gt		1256			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		452		762		05		gt		8070		Gundersen et al 1994		gt		1140			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		895		758		05		gt		8160		Gundersen et al 1994		gt		1489			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		1304		758		05		gt		8200		Gundersen et al 1994		gt		1676			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		232		825		05			6170		Gundersen et al 1994			260			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		350		826		05			6170		Gundersen et al 1994			333			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		836		829		05			7670		Gundersen et al 1994			701			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		1158		830		05			6930		Gundersen et al 1994			777			801		801			Oncorhynchus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Atlantic salmon Salmo salar		S U T		68		55		05			584		Hamilton and Haines 1995			4602			-				Salmo			Value is from 2007 FW Cu AWQC Appendix C recommendation		Soft recon
	Atlantic salmon Salmo salar		S U T		68		65		05			599		Hamilton and Haines 1995			531			1564		1564			Salmo		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Soft recon
	Brook trout Salvelinus fontinalis		S U T		40		56		16			6530		Tandjung 1982			6947			-				Salvelinus			Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Brook trout Salvelinus fontinalis		S U T		18		56		16			3400		Tandjung 1982			6209			-				Salvelinus			Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Brook trout Salvelinus fontinalis		S U T		2		56		16			370		Tandjung 1982			2988			5051		5051			Salvelinus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Green sunfish Lepomis cyanellus		S M T		474		755		11		gt		50000		Call et al 1984		gt		5255		gt		5255		5255		gt		Lepomis		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Rio Grande silvery minnow Hybognathus amarus		R M T		140		81		05		gt		59100		Buhl 2002		gt		8620		gt		8620		8620		gt		Hybognathus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon water
	Fathead minnow Pimephales promelas		S M T		474		761		11		gt		48200		Call et al 1984		gt		4707			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Fathead minnow Pimephales promelas		S M T		474		805		11		gt		49800		Call et al 1984		gt		2837			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Fathead minnow Pimephales promelas		R M T		26		78		05			1160		ENSR 1992c			104			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		46		76		05			8180		ENSR 1992c			1191			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		96		81		05			20300		ENSR 1992c			2398			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		194		81		05			44800		ENSR 1992c			7839			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		140		81		05		gt		59100		Buhl 2002		gt		8620			2224		2224			Pimephales		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon water
	Smallmouth bass Micropterus dolomieui		S M T		1245		505		16			130		Kane and Rabeni 1987			859			859		859			Micropterus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Green tree frog Hyla cinerea		R M T		455		549		05		gt		4052		Jung and Jagoe 1995		gt		4377		gt		4377		4377		gt		Hyla		Amphibian		Value is from 2007 FW Cu AWQC Appendix C recommendation		artificial soft recon

	Normalization Chemistry
	pH		6					FAV		360
	Hardness		25					CMC		180
	DOC		1					CCC		99
	Acute Ranked GMAV											Chronic Ranked GMCV
	Rank			GMAV		Genus								Rank			GMCV		Genus
	18		gt		8620		Hybognathus		Fish							12		gt		2131		Rana		Amphib - Other Data
	17		gt		6232		Paratanytarsus		Invert							11			1710		Aeolosoma		Invert
	16		gt		5255		Lepomis		Fish							10			591		Chironomus		Invert
	15			5051		Salvelinus		Fish							9			544		Pimephales		Fish
	14		gt		4377		Hyla		Amphibian							8			488		Lymnaea		Mollusk
	13			3546		Physa		Mollusk							7			255		Brachionus		Invert
	12		gt		2983		Lampsilis		Mollusk							6			241		Danio		Fish
	11		gt		2800		Hyalella		Invert							5			171		Hyalella		Invert
	10			2536		Chironomus		Invert							4			129		Salvelinus		Fish
	9			2224		Pimephales		Fish							3			118		Ceriodaphnia		Invert
	8		gt		2050		Acroneuria		Invert							2			111		Salmo		Fish
	7			1564		Salmo		Fish							1			104		Lampsilis		Mollusk
	6			1217		Crangonyx		Invert
	5			1022		Stenocypris		Invert
	4			859		Micropterus		Fish
	3			801		Oncorhynchus		Fish
	2			733		Ceriodaphnia		Invert
	1			333		Daphnia		Invert
		N		Rank		Genus		GMAV		ln(GMAV)		ln(GMAV)2		P=R(N+1)		sqrt(P)				N		Rank		Genus		GMCV		ln(GMCV)		ln(GMCV)2		P=R(N+1)		sqrt(P)
		18		4		Micropterus		859		676		4564		0211		0459				12		4		Salvelinus		129		486		2364		0308		0555
			3		Oncorhynchus		801		669		4469		0158		0397					3		Ceriodaphnia		118		477		2277		0231		0480
			2		Ceriodaphnia		733		660		4352		0105		0324					2		Salmo		111		471		2220		0154		0392
			1		Daphnia		333		581		3374		0053		0229					1		Lampsilis		104		465		2159		0077		0277
			Sum				2585		1676		0526		141					Sum				1899		902		0769		170
			S2 =		1987										S2 =		059
			L =		4890										L =		4420
			A =		5887										A =		4592
			FAV =		360										FCV =		987
			CMC =		180

				All concentrations reported are microgL total Aluminum
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	ENTER DATA HERE									ERRORNUM		ACUTE														CHRONIC
										Rank 1				Rank 2				Rank 3				Rank 4					Rank 1				Rank 2				Rank 3				Rank 4
	SiteName		DOC(mgL)		Hardness(mgL as CaCO3)		pH			FAV		CMC		CCC			Genus		GMAV			Genus		GMAV			Genus		GMAV			Genus		GMAV				Genus		GMCV			Genus		GMCV			Genus		GMCV			Genus		GMCV
	Site 1		50		25		9		75428737034162		35229324666008		1800		770			Daphnia			33452		Ceriodaphnia			73576		Stenocypris			102576		Crangonyx			122230			Lampsilis			10463		Ceriodaphnia			11865		Hyalella			17200		Brachionus			25648
	Site 2		50		50		9		75428737034162		31204991509063		1600		680			Daphnia			29631		Ceriodaphnia			65171		Stenocypris			90859		Crangonyx			108267			Lampsilis			9268		Ceriodaphnia			10510		Hyalella			15236		Brachionus			22718
	Site 3		50		75		9		75428737034162		29067527928199		1500		640			Daphnia			27601		Ceriodaphnia			60707		Stenocypris			84635		Crangonyx			100851			Lampsilis			8633		Ceriodaphnia			9790		Hyalella			14192		Brachionus			21162
	Site 4		50		100		9		75428737034162		27640367912592		1400		600			Daphnia			26246		Ceriodaphnia			57726		Stenocypris			80480		Crangonyx			95899			Lampsilis			8209		Ceriodaphnia			9309		Hyalella			13495		Brachionus			20123
	Site 5		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 6		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 7		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 8		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 9		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 10		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 11		50		25		85		75428737034162		63245275664384		3200		1400			Daphnia			60055		Ceriodaphnia			132086		Stenocypris			184149		Crangonyx			219432			Lampsilis			18783		Ceriodaphnia			21301		Hyalella			30879		Brachionus			46045
	Site 12		50		50		85		75428737034162		61388044609926		3100		1300			Daphnia			58291		Ceriodaphnia			128208		Stenocypris			178742		Crangonyx			212988			Lampsilis			18232		Ceriodaphnia			20676		Hyalella			29972		Brachionus			44693
	Site 13		50		75		85		75428737034162		60327020429132		3000		1300			Daphnia			57284		Ceriodaphnia			125992		Stenocypris			175652		Crangonyx			209307			Lampsilis			17917		Ceriodaphnia			20318		Hyalella			29454		Brachionus			43920
	Site 14		50		100		85		75428737034162		59585352130146		3000		1300			Daphnia			56580		Ceriodaphnia			124443		Stenocypris			173493		Crangonyx			206734			Lampsilis			17696		Ceriodaphnia			20068		Hyalella			29092		Salmo			41264
	Site 15		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 16		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 17		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 18		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 19		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 20		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370

	Appendix C Acceptable Chronic Toxicity Data of Aluminum to Freshwater Aquatic Animals
									MLR equation
	Species		Hardness (mgL as CaCO3)		pH		DOC(mgL)		EC20 Endpoint			EC20(microgL)			Normalized Conc- EC20(microgL)			SMCV-EC20(microgL)		GMCV-EC20(microgL)			Genus			DOC Notes		Dilution water
	Oligochaete Aeolosoma sp		48		6		025		Reproduction (population size)			1235			17098			17098		17098			Aeolosoma		Invert		Author reported half the detection limit
	Rotifer Brachionus calyciflorus		100		62		025		Reproduction (population size)			431			2555			2555		2555			Brachionus		Invert		Author reported half the detection limit
	Great pond snail Lymnaea stagnalis		117		61		025		Biomass			746			4877			4877		4877			Lymnaea		Mollusk		Author reported half the detection limit
	Fatmucket Lampsilis siliquoidea		1055		604		040		Biomass			169			1042			1042		1042			Lampsilis		Mollusk		Author reported
	Cladoceran Ceriodaphnia dubia		50		715		11		Reproduction - youngstarting adult			1780			1898						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		26		73		05		Reproduction - youngfemale			1557			2718						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		46		75		05		Reproduction - youngfemale			8087			1102						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		96		79		05		Reproduction - youngfemale			6519			796						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		194		81		05		Reproduction - youngfemale			6836			888						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		255		703		05		Reproduction (youngfemale)			250			563						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		122		713		05		Reproduction (youngfemale)			860			1061						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		25		798		05		Reproduction (youngfemale)			700			1006						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		61		803		05		Reproduction (youngfemale)			1010			1363						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		122		810		05		Reproduction (youngfemale)			870			1164						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		255		633		05		Reproduction (youngfemale)			260			1758						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		121		636		05		Reproduction (youngfemale)			390			1101			1182		1182			Ceriodaphnia		Invert		Author reported
	Amphipod Hyalella azteca		95		61		051		Biomass (28 d)			1993			1014						Hyalella			Author reported
	Amphipod Hyalella azteca		106		604		033		Biomass (28 d)			425			2895			1713		1713			Hyalella		Invert		Author reported
	Midge Chironomus riparius		118		558		18		Adult midge emergence			2955			1192						Chironomus			Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Midge Chironomus riparius		119		505		18		Adult midge emergence			8442			22578						Chironomus			Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Midge Chironomus riparius		91		66		051		Reproduction ( of eggscase)			3387			7664			5908		5908			Chironomus		Invert		Author reported
	Atlantic salmon Salmo salar		127		57		18		Biomass			6156			5085			5085		5085			Salmo		Fish		Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Brook trout Salvelinus fontinalis		123		655		19		Biomass			1644			270						Salvelinus			Author reported		wellRO mix
	Brook trout Salvelinus fontinalis		128		565		18		Biomass			1435			1295			5914		5914			Salvelinus		Fish		Author reported		wellRO mix
	Fathead minnow Pimephales promelas		220		770		16		Biomass			6194			3569						Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		well water
	Fathead minnow Pimephales promelas		96		620		025		Fry survival			4286			1734			2488		2488			Pimephales		Fish		Author reported half the detection limit
	Zebrafish Danio rerio		83		61		025		Biomass			2344			1102			1102		1102			Danio		Fish		Author reported half the detection limit
	Wood frog Rana sylvatica		115		469		16		MATC-survival		gt		2000		gt		9746		gt		9746		9746		gt		Rana		Amphib - Other Data		Value is from 2007 FW Cu AWQC Appendix C recommendation		aged tap water

	Appendix A Acceptable Acute Toxicity Data of Aluminum to Freshwater Aquatic Animals
	Species		Methoda		Hardness (mgL as CaCO3)		pH		DOC(mgL)			LC50 or EC50 Conc (microgL)		Reference			Normalized Conc - LC50(microgL)			Species Mean Acute Value(microgL)		Genus Mean Acute Value(microgL)			Genus			DOC Note		Dilution water
	Snail Physa sp		S M T		474		659		11		gt		23400		Call 1984 Call et al 1984		gt		48425			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		755		11			30600		Call 1984 Call et al 1984			26986			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		817		11		gt		24700		Call 1984 Call et al 1984		gt		24040			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		746		11			55500		Call 1984 Call et al 1984			50338			35462		35462			Physa		Mollusk		Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Fatmucket Lampsilis siliquoidea		F M T		1060		612		048		gt		6302		Wang et al 2016 Wang et al 2017		gt		29834		gt		29834		29834		gt		Lampsilis		Mollusk		Author reported
	Cladoceran Ceriodaphnia dubia		S M A		500		742		11			1900		McCauley et al 1986			1728			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		S M A		505		786		11			1500		McCauley et al 1986			1294			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		UW-S Lake Superior lab water
	Cladoceran Ceriodaphnia dubia		S M A		500		813		11			2560		McCauley et al 1986			2431			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		R M T		26		75		05			720		ENSR 1992d			1113			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		R M T		46		76		05			1880		ENSR 1992d			2495			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		R M T		96		78		05			2450		ENSR 1992d			2894			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		S U NR		90		715		05			3727		Fort and Stover 1995			5002			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U NR		90		715		05			5673		Fort and Stover 1995			7613			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U NR		89		76		05			2880		Soucek et al 2001			3362			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		601		05			7112		European Al Association 2009			1691			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		605		2			6865		European Al Association 2009			7088			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		609		4			15581		European Al Association 2009			10072			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		601		05			681		European Al Association 2009			1620			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		603		05			1630		European Al Association 2009			3674			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		597		05			1785		European Al Association 2009			4732			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		592		05			1410		European Al Association 2009			4295			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		699		05		gt		1300		European Al Association 2009		gt		4189			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		785		05		gt		5000		European Al Association 2009		gt		7960			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		68		2		gt		10000		European Al Association 2009		gt		20755			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		782		2		gt		15000		European Al Association 2009		gt		11626			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		677		4		gt		10000		European Al Association 2009		gt		15161			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		766		4		gt		15000		European Al Association 2009		gt		8608			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		79		05		gt		2000		European Al Association 2009		gt		3150			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		789		05		gt		2000		European Al Association 2009		gt		3156			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		604		05			1108		European Al Association 2009			850			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		598		2			11371		European Al Association 2009			4817			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		573		4			80467		European Al Association 2009			43570			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		671		05		gt		10000		European Al Association 2009		gt		23786			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		783		05		gt		5000		European Al Association 2009		gt		6344			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		679		2		gt		10000		European Al Association 2009		gt		10394			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		767		2		gt		15000		European Al Association 2009		gt		9082			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		668		4		gt		15000		European Al Association 2009		gt		12461			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		762		4		gt		15000		European Al Association 2009		gt		6332			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		606		2			33868		European Al Association 2009			7920			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		56		4			104842		European Al Association 2009			48800			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		693		05		gt		5000		European Al Association 2009		gt		7126			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		120		788		05		gt		5000		European Al Association 2009		gt		5898			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		120		676		2		gt		15000		European Al Association 2009		gt		12117			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		771		2		gt		15000		European Al Association 2009		gt		8096			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		66		4		gt		15000		European Al Association 2009		gt		10139			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		76		4		gt		15000		European Al Association 2009		gt		5545			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			11971		European Al Association 2010			2699			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			27478		European Al Association 2010			6194			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			11998		European Al Association 2010			2705			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		607		05			92495		European Al Association 2010			1876			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		609		05			31337		European Al Association 2010			6035			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		61		05			33235		European Al Association 2010			6238			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		708		05		gt		8864		European Al Association 2010		gt		2534			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		779		05		gt		42783		European Al Association 2010		gt		6930			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		753		05			13204		European Al Association 2010			244			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		600		601		05			46326		European Al Association 2010			3798			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		600		599		05		gt		8590		European Al Association 2010		gt		7366			5094				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia reticulata		F M T		451		60		11			304		Shephard 1983			2009			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia reticulata		F M T		40		55		11			362		Shephard 1983			55307			10542		7328			Ceriodaphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake SuperiorDI Mix
	Cladoceran Daphnia magna		S U NR		453		774		11			3900		Biesinger and Christensen 1972			3382			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Daphnia magna		S M T		220		76		16			38200		Kimball 1978			20303			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Well water
	Cladoceran Daphnia magna		S U T		451		725		11			2800		Shephard 1983			2868			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Daphnia magna		S U T		168		599		05			5000		European Al Association 2009		gt		2265			-				Daphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Daphnia magna		S U T		168		792		05			7950		European Al Association 2009			920			3332		3332			Daphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Ostracod Stenocypris major		R M T		1563		651		16			310196		Shuhaimi-Othman et al 2011a			10216			10216		10216			Stenocypris		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (Malaysia)
	Amphipod Crangonyx pseudogracilis		R U T		50		675		16			9190		Martin and Holdich 1986			12174			12174		12174			Crangonyx		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (UK)
	Amphipod Hyalella azteca		F M T		105		613		048		gt		5997		Wang et al 2016 Wang et al 2017		gt		28002		gt		28002		28002		gt		Hyalella		Invert		Author reported
	Stonefly Acroneuria sp		S M T		474		746		11		gt		22600		Call et al 1984		gt		20498		gt		20498		20498		gt		Acroneuria		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Midge Chironomus plumosus		S U T		80		70		16			30000		Fargasova 2001			25361			25361		25361			Chironomus		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (Slovakia)
	Midge Paratanytarsus dissimilis		S M T		1743		728		28		gt		77700		Lamb and Bailey 1981 1983		gt		62318		gt		62318		62318		gt		Paratanytarsus		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Liberty Lake Washington
	Rainbow trout Oncorhynchus mykiss		F M T		274		758		05		gt		9840		Gundersen et al 1994		gt		5745			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		452		762		05		gt		8070		Gundersen et al 1994		gt		5212			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		895		758		05		gt		8160		Gundersen et al 1994		gt		6807			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		1304		758		05		gt		8200		Gundersen et al 1994		gt		7662			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		232		825		05			6170		Gundersen et al 1994			1190			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		350		826		05			6170		Gundersen et al 1994			1523			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		836		829		05			7670		Gundersen et al 1994			3205			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		1158		830		05			6930		Gundersen et al 1994			3554			3661		3661			Oncorhynchus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Atlantic salmon Salmo salar		S U T		68		55		05			584		Hamilton and Haines 1995			21042			-				Salmo			Value is from 2007 FW Cu AWQC Appendix C recommendation		Soft recon
	Atlantic salmon Salmo salar		S U T		68		65		05			599		Hamilton and Haines 1995			2430			7151		7151			Salmo		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Soft recon
	Brook trout Salvelinus fontinalis		S U T		40		56		16			6530		Tandjung 1982			31767			-				Salvelinus			Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Brook trout Salvelinus fontinalis		S U T		18		56		16			3400		Tandjung 1982			28391			-				Salvelinus			Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Brook trout Salvelinus fontinalis		S U T		2		56		16			370		Tandjung 1982			13663			23097		23097			Salvelinus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Green sunfish Lepomis cyanellus		S M T		474		755		11		gt		50000		Call et al 1984		gt		24028		gt		24028		24028		gt		Lepomis		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Rio Grande silvery minnow Hybognathus amarus		R M T		140		81		05		gt		59100		Buhl 2002		gt		39414		gt		39414		39414		gt		Hybognathus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon water
	Fathead minnow Pimephales promelas		S M T		474		761		11		gt		48200		Call et al 1984		gt		21522			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Fathead minnow Pimephales promelas		S M T		474		805		11		gt		49800		Call et al 1984		gt		12972			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Fathead minnow Pimephales promelas		R M T		26		78		05			1160		ENSR 1992c			477			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		46		76		05			8180		ENSR 1992c			5446			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		96		81		05			20300		ENSR 1992c			10966			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		194		81		05			44800		ENSR 1992c			35847			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		140		81		05		gt		59100		Buhl 2002		gt		39414			10168		10168			Pimephales		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon water
	Smallmouth bass Micropterus dolomieui		S M T		1245		505		16			130		Kane and Rabeni 1987			3929			3929		3929			Micropterus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Green tree frog Hyla cinerea		R M T		455		549		05		gt		4052		Jung and Jagoe 1995		gt		20016		gt		20016		20016		gt		Hyla		Amphibian		Value is from 2007 FW Cu AWQC Appendix C recommendation		artificial soft recon

	Normalization Chemistry
	pH		7					FAV		2741
	Hardness		100					CMC		1400
	DOC		1					CCC		390
	Acute Ranked GMAV											Chronic Ranked GMCV
	Rank			GMAV		Genus								Rank			GMCV		Genus
	18		gt		62318		Paratanytarsus		Invert							12			17098		Aeolosoma		Invert
	17		gt		39414		Hybognathus		Fish							11		gt		9746		Rana		Amphib - Other Data
	16			35462		Physa		Mollusk							10			5908		Chironomus		Invert
	15		gt		29834		Lampsilis		Mollusk							9			4877		Lymnaea		Mollusk
	14		gt		28002		Hyalella		Invert							8			2555		Brachionus		Invert
	13			25361		Chironomus		Invert							7			2488		Pimephales		Fish
	12		gt		24028		Lepomis		Fish							6			1713		Hyalella		Invert
	11			23097		Salvelinus		Fish							5			1182		Ceriodaphnia		Invert
	10		gt		20498		Acroneuria		Invert							4			1102		Danio		Fish
	9		gt		20016		Hyla		Amphibian							3			1042		Lampsilis		Mollusk
	8			12174		Crangonyx		Invert							2			591		Salvelinus		Fish
	7			10216		Stenocypris		Invert							1			509		Salmo		Fish
	6			10168		Pimephales		Fish
	5			7328		Ceriodaphnia		Invert
	4			7151		Salmo		Fish
	3			3929		Micropterus		Fish
	2			3661		Oncorhynchus		Fish
	1			3332		Daphnia		Invert
		N		Rank		Genus		GMAV		ln(GMAV)		ln(GMAV)2		P=R(N+1)		sqrt(P)				N		Rank		Genus		GMCV		ln(GMCV)		ln(GMCV)2		P=R(N+1)		sqrt(P)
		18		4		Salmo		7151		887		7876		0211		0459				12		4		Danio		1102		700		4907		0308		0555
			3		Micropterus		3929		828		6849		0158		0397					3		Lampsilis		1042		695		4829		0231		0480
			2		Oncorhynchus		3661		821		6733		0105		0324					2		Salvelinus		591		638		4074		0154		0392
			1		Daphnia		3332		811		6579		0053		0229					1		Salmo		509		623		3883		0077		0277
			Sum				3347		2804		0526		141					Sum				2657		1769		0769		170
			S2 =		1223										S2 =		1080
			L =		7134										L =		5242
			A =		7916										A =		5976
			FAV =		2741										FCV =		3940
			CMC =		1371

				All concentrations reported are microgL total Aluminum
				lt-----Click Button after entering data
	ENTER DATA HERE									ERRORNUM		ACUTE														CHRONIC
										Rank 1				Rank 2				Rank 3				Rank 4					Rank 1				Rank 2				Rank 3				Rank 4
	SiteName		DOC(mgL)		Hardness(mgL as CaCO3)		pH			FAV		CMC		CCC			Genus		GMAV			Genus		GMAV			Genus		GMAV			Genus		GMAV				Genus		GMCV			Genus		GMCV			Genus		GMCV			Genus		GMCV
	Site 1		50		25		9		75428737034162		35229324666008		1800		770			Daphnia			33452		Ceriodaphnia			73576		Stenocypris			102576		Crangonyx			122230			Lampsilis			10463		Ceriodaphnia			11865		Hyalella			17200		Brachionus			25648
	Site 2		50		50		9		75428737034162		31204991509063		1600		680			Daphnia			29631		Ceriodaphnia			65171		Stenocypris			90859		Crangonyx			108267			Lampsilis			9268		Ceriodaphnia			10510		Hyalella			15236		Brachionus			22718
	Site 3		50		75		9		75428737034162		29067527928199		1500		640			Daphnia			27601		Ceriodaphnia			60707		Stenocypris			84635		Crangonyx			100851			Lampsilis			8633		Ceriodaphnia			9790		Hyalella			14192		Brachionus			21162
	Site 4		50		100		9		75428737034162		27640367912592		1400		600			Daphnia			26246		Ceriodaphnia			57726		Stenocypris			80480		Crangonyx			95899			Lampsilis			8209		Ceriodaphnia			9309		Hyalella			13495		Brachionus			20123
	Site 5		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 6		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 7		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 8		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 9		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 10		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 11		50		25		85		75428737034162		63245275664384		3200		1400			Daphnia			60055		Ceriodaphnia			132086		Stenocypris			184149		Crangonyx			219432			Lampsilis			18783		Ceriodaphnia			21301		Hyalella			30879		Brachionus			46045
	Site 12		50		50		85		75428737034162		61388044609926		3100		1300			Daphnia			58291		Ceriodaphnia			128208		Stenocypris			178742		Crangonyx			212988			Lampsilis			18232		Ceriodaphnia			20676		Hyalella			29972		Brachionus			44693
	Site 13		50		75		85		75428737034162		60327020429132		3000		1300			Daphnia			57284		Ceriodaphnia			125992		Stenocypris			175652		Crangonyx			209307			Lampsilis			17917		Ceriodaphnia			20318		Hyalella			29454		Brachionus			43920
	Site 14		50		100		85		75428737034162		59585352130146		3000		1300			Daphnia			56580		Ceriodaphnia			124443		Stenocypris			173493		Crangonyx			206734			Lampsilis			17696		Ceriodaphnia			20068		Hyalella			29092		Salmo			41264
	Site 15		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 16		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 17		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 18		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 19		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 20		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370

Criteria Development Processbull All EPA Aquatic Life Criteria for Toxics undergo rigorous

scientific development and review processbull Develop criteria document draftbull Intra-agency peer review process bull Independent external peer reviewbull Revisions based on external peer review

Intra-agency review of draft criteria documentbull Release to the public in draft form to obtain scientific views

Revisions considering public comments Intra-agency review of final criteria document

bull Publication of final criteria document bull States may then consider recommended criteria in

development and adoption of state water quality standards8

Framework for Updated Draft Aluminum Criteria

I Screen toxicological studies for high

quality data

II Consider water conditions effects on

aluminum bioavailability

III Normalize toxicity data in species

sensitivity distribution to a set of water quality conditions

IV Construct sensitivity distribution to determine criteria

at set conditions

9

I Screen toxicological studies for high quality data

bull First step in all criteria developmentndash Data are evaluated based on the data quality objectives

outlined in the 1985 Guidelines methodology and other toxicity testing recommendations (ie EPA ASTM)

ndash High quality data are used quantitatively to develop criteria magnitudes

ndash Lower quality data are used qualitatively if appropriatendash Lowest quality data are considered unacceptable and not

usedbull Data are grouped into acute and chronic exposures

10

Aluminum Toxicity Databull The updated freshwater aluminum criteria meets the

acute and chronic minimum data requirements outlined in EPArsquos 1985 Guidelines

InvertebrateSpecies

Fish Species

AmphibianSpecies

Total Genera

Acute1988 AWQC 8 7 - 142017 Draft AWQC 11 8 1 18

Chronic1988 AWQC 2 1 - 32017 Draft AWQC 7 4 - 11

11 1988 AWQC Chronic CCC based on two studies for the striped

bass and brook trout

Aluminum Toxicity Data Freshwater mussels have been

suggested to be potentially sensitive to aluminum

EPA worked collaboratively with USGSrsquos Columbia Environmental Research Center (CERC) to have acute and chronic aluminum toxicity tests conducted with a unionid mussel (fatmucket Lampsilis siliquoidea) in water-only exposures to obtain additional data on invertebrates New data on amphipod (Hyalella azteca)

were collected to confirm laboratory conditions

12

Aluminum Toxicity Data

13

bull New data and models examinedndash Data submitted to EU REACH program

bull New freshwater aluminum toxicity data generated by Oregon State University

bull 2016 BLM model provided by the European Aluminum Association

ndash Saltwater data provided by Australia bull Insufficient data to develop estuarinemarine acute or chronic

criteria at this timendash Multiple Linear Regression (MLR) model developed by

DeForest et al 2017bull DeForest DK KV Brix LM Tear and WJ Adams 2017 Multiple Linear Regression (MLR) models for predicting chronic aluminum

toxicity to freshwater aquatic organisms and developing water quality guidelines Environ Toxicol Chem (DOI101002etc3922)

14

Minimum Dataset For Freshwater Criteria Derivation

SALMONID SECONDFISHFAMILY

CHORDATA

PLANKTONICCRUSTACEAN

BENTHIC CRUSTACEAN

INSECT ROTIFERA ANNELIDA MOLLUSCA

OTHERINSECT ORMOLLUSCA

EstuarineMarine Minimum Data Requirements

15

Family Minimum Data Requirement EstuarineMarine

Acute(Phylum Family Genus)

Chronic(Phylum Family Genus)

Family in the phylum Chordata - -

Family in the phylum Chordata - -

Either the Mysidae or Penaeidae family - -

Family in a phylum other than Arthropoda or Chordata Mollusca Ostreidae Crassostrea -

Family in a phylum other than Chordata Annelida Nereididae Neanthes -

Family in a phylum other than Chordata Annelida Capitellidae Capitella -

Family in a phylum other than Chordata Annelida Ctenodrilidae Ctenodrilus -

Any other family Arthropoda Ameiridae Nitokra -

II Evaluate water quality conditions to determine applicability

bull Criteria are often based on one or more water quality conditions (eg hardness) that affect toxicity of the material to test organisms

bull Aluminum toxicity is highly dependent on the pH of the test waterndash The pH of test waters affects the amount and form

of aluminum that is bioavailable to test speciesndash Hardness and DOC (Dissolved Organic Carbon)

also influence the amount of aluminum that is bioavailable

16

Multiple Bioavailability Modeling Approaches Underwent External Peer Review

17

bull Biotic Ligand Modelsbull Full aluminum BLM (2016 model version) ndash Temperature pH

Dissolved organic carbon (DOC) Major cations (calcium [Ca] magnesium [Mg] sodium [Na] and potassium [K]) Major anions (sulfate [SO4] and chloride [Cl]) Alkalinity and Sulfide

bull Simplified aluminum BLM - pH hardness DOC temperature

bull Multilinear Regression Equationsbull pH and Hardness

Accounting for Effects of Water Chemistry on Bioavailability and Toxicity

bull Water quality criteria can be modified quantitatively when enough data are available to demonstrate that those conditions similarly affect the results with a variety of speciesndash High quality data in the draft aluminum document demonstrated

that pH DOC and hardness affected a variety of species in a similar way when exposed to aluminum concentrations

bull Recent publications by Cardwell et al (2017) and Gensemer et al (2017) summarized short-term aluminum chronic toxicity data across a range of DOC pH and hardness values for three species

18

Multiple Linear Regression (MLR) Models to Account for Effects of Water Chemistry

bull MLR models were developed for each species (DeForest et al 2017)ndash 119862119862 119889119889119906119906119887119887119894119894119886119886 11986411986411986211986220=119890119890[minus41026+[0525timesln(119863119863119874119874119862119862)]+[2201timesln(ℎ119886119886119903119903119889119889)]+(11282times119901119901119867119867)minus(0633times1199011199011198671198672)minus[0264times119901119901119867119867ln(ℎ119886119886119903119903119889119889)]]

ndash 119875119875 119901119901119903119903119900119900119898119898119890119890119897119897119886119886119904119904 11986411986411986211986220=119890119890[minus14029+[0503timesln(119863119863119874119874119862119862)]+[3443timesln(ℎ119886119886119903119903119889119889)]+(3131times119901119901119867119867)minus[0494times119901119901119867119867ln(ℎ119886119886119903119903119889119889)]]

bull These models were applied to both the acute and chronic aluminum toxicity datandash The C dubia model was applied to invertebrate datandash The P promelas model was applied to vertebrate data

19

III Normalize toxicity data in species sensitivity distribution to a set of water

quality conditionsbull Just like single parameter hardness models the MLR

models were used to normalize the aluminum toxicity data to a single set of water quality conditionsndash As an illustration for one set of calculations the draft

aluminum AWQC set these conditions to pH 7 hardness of 100 mgL and DOC of 1 mgL

bull This allows for comparisons to be made between species based on their sensitivity to aluminumndash Apples to apples

20

IV Construct sensitivity distribution to determine criteria at set conditions

21

Figure 8 Ranked Summary of Total Aluminum Genus Mean Acute Values (GMAVs) - Freshwater at pH 7 Hardness of 100 mgL and DOC of 1 mgL

IV Construct sensitivity distribution to determine criteria at set conditions

22

Figure 10 Ranked Summary of Total Aluminum Genus Mean Chronic Values (GMCVs) ndash Freshwater Supplemented with Other Data to Fulfill Missing MDRs at pH 7 Hardness of 100 mgL and DOC of 1 mgL

Aluminum Criteria Calculatorbull The relative ranking of genera in the sensitivity

distribution to which the toxicity data are normalized will change based on the set of water chemistry conditions (ie pH hardness and DOC)

bull To make calculating aluminum criteria easier EPA developed an Aluminum Criteria Calculatorndash Automated so that user only needs to enter water chemistry

conditions of interestndash Allows quick calculations for a variety of conditionsndash Limits calculations to the recommended conditions for which

the MLR models were developed were specified for hardness and DOC

bull No extrapolation beyond hardness and DOC empirical data bounds

23

Aluminum Criteria Calculator SD Example

24

pH=7 Hardness=100 DOC=1 pH=6 Hardness=25 DOC=1

Chronic Ranked GMCVRank GMCV Genus

12 17098 Aeolosoma Invert11 gt 9746 Rana Amphib - Other Data10 5908 Chironomus Invert

9 4877 Lymnaea Mollusk8 2555 Brachionus Invert7 2488 Pimephales Fish6 1713 Hyalella Invert5 1182 Ceriodaphnia Invert4 1102 Danio Fish3 1042 Lampsilis Mollusk2 591 Salvelinus Fish1 509 Salmo Fish

FCV = 3940 microgL total aluminum

Chronic Ranked GMCVRank GMCV Genus

12 gt 2131 Rana Amphib - Other Data11 1710 Aeolosoma Invert10 591 Chironomus Invert

9 544 Pimephales Fish8 488 Lymnaea Mollusk7 255 Brachionus Invert6 241 Danio Fish5 171 Hyalella Invert4 129 Salvelinus Fish3 118 Ceriodaphnia Invert2 111 Salmo Fish1 104 Lampsilis Mollusk

FCV = 987 microgL total aluminum

Multiple Scenarios

Run Scenarios

Summary Sheet

Acute Dataset

Chronic Dataset

Multiple Scenarios

Run Scenarios

Summary Sheet

Acute Dataset

Chronic Dataset

Boundaries of the Criteria Outputsbull MLR models were developed using data that

encompassndash DOC range of 008-5 mgLndash Hardness range of 98-127 mgL (as CaCO3)ndash pH range of 6-81

bull MLR criteria outputs are bounded atndash Maximum of 150 mgL total hardness (as CaCO3)

ndash DOC of 50 mgLndash pH range of the model is 50 to 90

25

Aluminum Criteria Look-Up Tables

26

bull EPA also provided criteria look-up tables in Appendix K

Har

dnes

s

CCC(microgL total aluminum)

(DOC=05 mgL)

pH 50 pH 55 pH 60 pH 65 pH 70 pH 75 pH 80 pH 85 pH 90

25 23 a 14 b 69 c 160 f 350 f 620 i 540 b 410 a 230 a

50 43 a 24 b 100 d 170 f 310 f 630 f 670 j 400 a 200 a

75 61 a 33 b 130 e 180 f 290 f 530 f 730 i 390 a 190 a

100 78 a 41 b 150 e 190 f 280 g 460 f 770 c 390 a 180 a

150 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

200 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

250 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

300 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

350 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

400 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

Aluminum Criteria Look-Up Tables

27

bull EPA also provided criteria look-up tables in Appendix K

Har

dnes

s

CCC(microgL total aluminum)

(DOC=10 mgL)

pH 50 pH 55 pH 60 pH 65 pH 70 pH 75 pH 80 pH 85 pH 90

25 33 a 21 b 99 c 220 f 490 f 900 i 780 b 590 a 330 a

50 61 a 35 b 150 e 250 f 440 f 890 f 960 j 580 a 290 a

75 88 a 47 b 180 e 260 f 410 f 740 f 1100 i 570 a 270 a

100 11 a 59 b 210 e 270 f 390 g 650 f 1100 c 560 b 260 a

150 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

200 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

250 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

300 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

350 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

400 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

Aluminum Criteria Look-Up Tables

28

bull EPA also provided criteria look-up tables in Appendix K

Har

dnes

s

CCC(microgL total aluminum)

(DOC=25 mgL)

pH 50 pH 55 pH 60 pH 65 pH 70 pH 75 pH 80 pH 85 pH 90

25 54 a 34 b 160 c 350 f 770 f 1500 i 1300 b 960 a 540 a

50 99 a 57 b 230 e 390 f 690 f 1400 f 1600 j 930 a 470 a

75 14 a 77 b 290 e 410 f 640 f 1200 f 1700 i 920 a 440 a

100 18 a 96 b 330 e 420 f 620 g 1000 f 1800 d 920 b 420 a

150 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

200 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

250 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

300 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

350 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

400 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

Aluminum Criteria Look-Up Tables

29

bull EPA also provided criteria look-up tables in Appendix K

Har

dnes

s

CCC(microgL total aluminum)

(DOC=50 mgL)

pH 50 pH 55 pH 60 pH 65 pH 70 pH 75 pH 80 pH 85 pH 90

25 77 a 49 b 230 c 500 f 1100 f 2100 c 1900 b 1400 a 770 a

50 14 a 82 b 330 e 540 f 970 f 2000 f 2200 j 1300 a 680 a

75 20 a 110 b 410 e 570 f 900 f 1600 f 2500 i 1300 a 640 a

100 26 a 140 b 460 f 590 f 880 g 1400 f 2500 d 1300 b 600 a

150 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

200 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

250 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

300 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

350 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

400 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

Status

30

bull Draft Document Released to the Federal Register on 72817ndash Public comment period of 60 days ndash Due Date for Comments is 92617

bull Revisions will be made considering public commentsbull Subsequent EPA intra-agency review of final criteria

document will then be conducted bull Publication of final criteria document planned for Late Fall

2017

Federal Register Webpagehttpswwwfederalregistergovdocuments201

707282017-15968request-for-scientific-views-draft-updated-aquatic-life-ambient-water-quality-criteria-for-aluminum

Aluminum Criteria Webpagehttpswwwepagovwqc2017-draft-aquatic-life-

criteria-aluminum-freshwater-documents

31

	Appendix C Acceptable Chronic Toxicity Data of Aluminum to Freshwater Aquatic Animals
									MLR equation
	Species		Hardness (mgL as CaCO3)		pH		DOC(mgL)		EC20 Endpoint			EC20(microgL)			Normalized Conc- EC20(microgL)			SMCV-EC20(microgL)		GMCV-EC20(microgL)			Genus			DOC Notes		Dilution water
	Oligochaete Aeolosoma sp		48		6		025		Reproduction (population size)			1235			1710			1710		1710			Aeolosoma		Invert		Author reported half the detection limit
	Rotifer Brachionus calyciflorus		100		62		025		Reproduction (population size)			431			255			255		255			Brachionus		Invert		Author reported half the detection limit
	Great pond snail Lymnaea stagnalis		117		61		025		Biomass			746			488			488		488			Lymnaea		Mollusk		Author reported half the detection limit
	Fatmucket Lampsilis siliquoidea		1055		604		040		Biomass			169			104			104		104			Lampsilis		Mollusk		Author reported
	Cladoceran Ceriodaphnia dubia		50		715		11		Reproduction - youngstarting adult			1780			190						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		26		73		05		Reproduction - youngfemale			1557			272						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		46		75		05		Reproduction - youngfemale			8087			110						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		96		79		05		Reproduction - youngfemale			6519			80						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		194		81		05		Reproduction - youngfemale			6836			89						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		255		703		05		Reproduction (youngfemale)			250			56						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		122		713		05		Reproduction (youngfemale)			860			106						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		25		798		05		Reproduction (youngfemale)			700			101						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		61		803		05		Reproduction (youngfemale)			1010			136						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		122		810		05		Reproduction (youngfemale)			870			116						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		255		633		05		Reproduction (youngfemale)			260			176						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		121		636		05		Reproduction (youngfemale)			390			110			118		118			Ceriodaphnia		Invert		Author reported
	Amphipod Hyalella azteca		95		61		051		Biomass (28 d)			1993			101						Hyalella			Author reported
	Amphipod Hyalella azteca		106		604		033		Biomass (28 d)			425			289			171		171			Hyalella		Invert		Author reported
	Midge Chironomus riparius		118		558		18		Adult midge emergence			2955			119						Chironomus			Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Midge Chironomus riparius		119		505		18		Adult midge emergence			8442			2258						Chironomus			Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Midge Chironomus riparius		91		66		051		Reproduction ( of eggscase)			3387			766			591		591			Chironomus		Invert		Author reported
	Atlantic salmon Salmo salar		127		57		18		Biomass			6156			1112			1112		1112			Salmo		Fish		Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Brook trout Salvelinus fontinalis		123		655		19		Biomass			1644			59						Salvelinus			Author reported		wellRO mix
	Brook trout Salvelinus fontinalis		128		565		18		Biomass			1435			283			1293		1293			Salvelinus		Fish		Author reported		wellRO mix
	Fathead minnow Pimephales promelas		220		770		16		Biomass			6194			781						Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		well water
	Fathead minnow Pimephales promelas		96		620		025		Fry survival			4286			379			544		544			Pimephales		Fish		Author reported half the detection limit
	Zebrafish Danio rerio		83		61		025		Biomass			2344			241			241		241			Danio		Fish		Author reported half the detection limit
	Wood frog Rana sylvatica		115		469		16		MATC-survival		gt		2000		gt		2131		gt		2131		2131		gt		Rana		Amphib - Other Data		Value is from 2007 FW Cu AWQC Appendix C recommendation		aged tap water

	Appendix A Acceptable Acute Toxicity Data of Aluminum to Freshwater Aquatic Animals
	Species		Methoda		Hardness (mgL as CaCO3)		pH		DOC(mgL)			LC50 or EC50 Conc (microgL)		Reference			Normalized Conc - LC50(microgL)			Species Mean Acute Value(microgL)		Genus Mean Acute Value(microgL)			Genus			DOC Note		Dilution water
	Snail Physa sp		S M T		474		659		11		gt		23400		Call 1984 Call et al 1984		gt		4843			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		755		11			30600		Call 1984 Call et al 1984			2699			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		817		11		gt		24700		Call 1984 Call et al 1984		gt		2404			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		746		11			55500		Call 1984 Call et al 1984			5034			3546		3546			Physa		Mollusk		Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Fatmucket Lampsilis siliquoidea		F M T		1060		612		048		gt		6302		Wang et al 2016 Wang et al 2017		gt		2983		gt		2983		2983		gt		Lampsilis		Mollusk		Author reported
	Cladoceran Ceriodaphnia dubia		S M A		500		742		11			1900		McCauley et al 1986			173			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		S M A		505		786		11			1500		McCauley et al 1986			129			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		UW-S Lake Superior lab water
	Cladoceran Ceriodaphnia dubia		S M A		500		813		11			2560		McCauley et al 1986			243			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		R M T		26		75		05			720		ENSR 1992d			111			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		R M T		46		76		05			1880		ENSR 1992d			250			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		R M T		96		78		05			2450		ENSR 1992d			289			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		S U NR		90		715		05			3727		Fort and Stover 1995			500			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U NR		90		715		05			5673		Fort and Stover 1995			761			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U NR		89		76		05			2880		Soucek et al 2001			336			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		601		05			7112		European Al Association 2009			169			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		605		2			6865		European Al Association 2009			709			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		609		4			15581		European Al Association 2009			1007			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		601		05			681		European Al Association 2009			162			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		603		05			1630		European Al Association 2009			367			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		597		05			1785		European Al Association 2009			473			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		592		05			1410		European Al Association 2009			429			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		699		05		gt		1300		European Al Association 2009		gt		419			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		785		05		gt		5000		European Al Association 2009		gt		796			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		68		2		gt		10000		European Al Association 2009		gt		2076			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		782		2		gt		15000		European Al Association 2009		gt		1163			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		677		4		gt		10000		European Al Association 2009		gt		1516			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		766		4		gt		15000		European Al Association 2009		gt		861			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		79		05		gt		2000		European Al Association 2009		gt		315			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		789		05		gt		2000		European Al Association 2009		gt		316			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		604		05			1108		European Al Association 2009			85			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		598		2			11371		European Al Association 2009			482			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		573		4			80467		European Al Association 2009			4357			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		671		05		gt		10000		European Al Association 2009		gt		2379			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		783		05		gt		5000		European Al Association 2009		gt		634			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		679		2		gt		10000		European Al Association 2009		gt		1039			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		767		2		gt		15000		European Al Association 2009		gt		908			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		668		4		gt		15000		European Al Association 2009		gt		1246			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		762		4		gt		15000		European Al Association 2009		gt		633			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		606		2			33868		European Al Association 2009			792			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		56		4			104842		European Al Association 2009			4880			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		693		05		gt		5000		European Al Association 2009		gt		713			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		120		788		05		gt		5000		European Al Association 2009		gt		590			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		120		676		2		gt		15000		European Al Association 2009		gt		1212			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		771		2		gt		15000		European Al Association 2009		gt		810			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		66		4		gt		15000		European Al Association 2009		gt		1014			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		76		4		gt		15000		European Al Association 2009		gt		555			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			11971		European Al Association 2010			270			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			27478		European Al Association 2010			619			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			11998		European Al Association 2010			270			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		607		05			92495		European Al Association 2010			188			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		609		05			31337		European Al Association 2010			604			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		61		05			33235		European Al Association 2010			624			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		708		05		gt		8864		European Al Association 2010		gt		253			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		779		05		gt		42783		European Al Association 2010		gt		693			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		753		05			13204		European Al Association 2010			24			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		600		601		05			46326		European Al Association 2010			380			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		600		599		05		gt		8590		European Al Association 2010		gt		737			509				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia reticulata		F M T		451		60		11			304		Shephard 1983			201			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia reticulata		F M T		40		55		11			362		Shephard 1983			5531			1054		733			Ceriodaphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake SuperiorDI Mix
	Cladoceran Daphnia magna		S U NR		453		774		11			3900		Biesinger and Christensen 1972			338			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Daphnia magna		S M T		220		76		16			38200		Kimball 1978			2030			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Well water
	Cladoceran Daphnia magna		S U T		451		725		11			2800		Shephard 1983			287			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Daphnia magna		S U T		168		599		05			5000		European Al Association 2009		gt		227			-				Daphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Daphnia magna		S U T		168		792		05			7950		European Al Association 2009			92			333		333			Daphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Ostracod Stenocypris major		R M T		1563		651		16			310196		Shuhaimi-Othman et al 2011a			1022			1022		1022			Stenocypris		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (Malaysia)
	Amphipod Crangonyx pseudogracilis		R U T		50		675		16			9190		Martin and Holdich 1986			1217			1217		1217			Crangonyx		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (UK)
	Amphipod Hyalella azteca		F M T		105		613		048		gt		5997		Wang et al 2016 Wang et al 2017		gt		2800		gt		2800		2800		gt		Hyalella		Invert		Author reported
	Stonefly Acroneuria sp		S M T		474		746		11		gt		22600		Call et al 1984		gt		2050		gt		2050		2050		gt		Acroneuria		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Midge Chironomus plumosus		S U T		80		70		16			30000		Fargasova 2001			2536			2536		2536			Chironomus		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (Slovakia)
	Midge Paratanytarsus dissimilis		S M T		1743		728		28		gt		77700		Lamb and Bailey 1981 1983		gt		6232		gt		6232		6232		gt		Paratanytarsus		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Liberty Lake Washington
	Rainbow trout Oncorhynchus mykiss		F M T		274		758		05		gt		9840		Gundersen et al 1994		gt		1256			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		452		762		05		gt		8070		Gundersen et al 1994		gt		1140			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		895		758		05		gt		8160		Gundersen et al 1994		gt		1489			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		1304		758		05		gt		8200		Gundersen et al 1994		gt		1676			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		232		825		05			6170		Gundersen et al 1994			260			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		350		826		05			6170		Gundersen et al 1994			333			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		836		829		05			7670		Gundersen et al 1994			701			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		1158		830		05			6930		Gundersen et al 1994			777			801		801			Oncorhynchus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Atlantic salmon Salmo salar		S U T		68		55		05			584		Hamilton and Haines 1995			4602			-				Salmo			Value is from 2007 FW Cu AWQC Appendix C recommendation		Soft recon
	Atlantic salmon Salmo salar		S U T		68		65		05			599		Hamilton and Haines 1995			531			1564		1564			Salmo		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Soft recon
	Brook trout Salvelinus fontinalis		S U T		40		56		16			6530		Tandjung 1982			6947			-				Salvelinus			Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Brook trout Salvelinus fontinalis		S U T		18		56		16			3400		Tandjung 1982			6209			-				Salvelinus			Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Brook trout Salvelinus fontinalis		S U T		2		56		16			370		Tandjung 1982			2988			5051		5051			Salvelinus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Green sunfish Lepomis cyanellus		S M T		474		755		11		gt		50000		Call et al 1984		gt		5255		gt		5255		5255		gt		Lepomis		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Rio Grande silvery minnow Hybognathus amarus		R M T		140		81		05		gt		59100		Buhl 2002		gt		8620		gt		8620		8620		gt		Hybognathus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon water
	Fathead minnow Pimephales promelas		S M T		474		761		11		gt		48200		Call et al 1984		gt		4707			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Fathead minnow Pimephales promelas		S M T		474		805		11		gt		49800		Call et al 1984		gt		2837			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Fathead minnow Pimephales promelas		R M T		26		78		05			1160		ENSR 1992c			104			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		46		76		05			8180		ENSR 1992c			1191			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		96		81		05			20300		ENSR 1992c			2398			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		194		81		05			44800		ENSR 1992c			7839			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		140		81		05		gt		59100		Buhl 2002		gt		8620			2224		2224			Pimephales		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon water
	Smallmouth bass Micropterus dolomieui		S M T		1245		505		16			130		Kane and Rabeni 1987			859			859		859			Micropterus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Green tree frog Hyla cinerea		R M T		455		549		05		gt		4052		Jung and Jagoe 1995		gt		4377		gt		4377		4377		gt		Hyla		Amphibian		Value is from 2007 FW Cu AWQC Appendix C recommendation		artificial soft recon

	Normalization Chemistry
	pH		6					FAV		360
	Hardness		25					CMC		180
	DOC		1					CCC		99
	Acute Ranked GMAV											Chronic Ranked GMCV
	Rank			GMAV		Genus								Rank			GMCV		Genus
	18		gt		8620		Hybognathus		Fish							12		gt		2131		Rana		Amphib - Other Data
	17		gt		6232		Paratanytarsus		Invert							11			1710		Aeolosoma		Invert
	16		gt		5255		Lepomis		Fish							10			591		Chironomus		Invert
	15			5051		Salvelinus		Fish							9			544		Pimephales		Fish
	14		gt		4377		Hyla		Amphibian							8			488		Lymnaea		Mollusk
	13			3546		Physa		Mollusk							7			255		Brachionus		Invert
	12		gt		2983		Lampsilis		Mollusk							6			241		Danio		Fish
	11		gt		2800		Hyalella		Invert							5			171		Hyalella		Invert
	10			2536		Chironomus		Invert							4			129		Salvelinus		Fish
	9			2224		Pimephales		Fish							3			118		Ceriodaphnia		Invert
	8		gt		2050		Acroneuria		Invert							2			111		Salmo		Fish
	7			1564		Salmo		Fish							1			104		Lampsilis		Mollusk
	6			1217		Crangonyx		Invert
	5			1022		Stenocypris		Invert
	4			859		Micropterus		Fish
	3			801		Oncorhynchus		Fish
	2			733		Ceriodaphnia		Invert
	1			333		Daphnia		Invert
		N		Rank		Genus		GMAV		ln(GMAV)		ln(GMAV)2		P=R(N+1)		sqrt(P)				N		Rank		Genus		GMCV		ln(GMCV)		ln(GMCV)2		P=R(N+1)		sqrt(P)
		18		4		Micropterus		859		676		4564		0211		0459				12		4		Salvelinus		129		486		2364		0308		0555
			3		Oncorhynchus		801		669		4469		0158		0397					3		Ceriodaphnia		118		477		2277		0231		0480
			2		Ceriodaphnia		733		660		4352		0105		0324					2		Salmo		111		471		2220		0154		0392
			1		Daphnia		333		581		3374		0053		0229					1		Lampsilis		104		465		2159		0077		0277
			Sum				2585		1676		0526		141					Sum				1899		902		0769		170
			S2 =		1987										S2 =		059
			L =		4890										L =		4420
			A =		5887										A =		4592
			FAV =		360										FCV =		987
			CMC =		180

				All concentrations reported are microgL total Aluminum
				lt-----Click Button after entering data
	ENTER DATA HERE									ERRORNUM		ACUTE														CHRONIC
										Rank 1				Rank 2				Rank 3				Rank 4					Rank 1				Rank 2				Rank 3				Rank 4
	SiteName		DOC(mgL)		Hardness(mgL as CaCO3)		pH			FAV		CMC		CCC			Genus		GMAV			Genus		GMAV			Genus		GMAV			Genus		GMAV				Genus		GMCV			Genus		GMCV			Genus		GMCV			Genus		GMCV
	Site 1		50		25		9		75428737034162		35229324666008		1800		770			Daphnia			33452		Ceriodaphnia			73576		Stenocypris			102576		Crangonyx			122230			Lampsilis			10463		Ceriodaphnia			11865		Hyalella			17200		Brachionus			25648
	Site 2		50		50		9		75428737034162		31204991509063		1600		680			Daphnia			29631		Ceriodaphnia			65171		Stenocypris			90859		Crangonyx			108267			Lampsilis			9268		Ceriodaphnia			10510		Hyalella			15236		Brachionus			22718
	Site 3		50		75		9		75428737034162		29067527928199		1500		640			Daphnia			27601		Ceriodaphnia			60707		Stenocypris			84635		Crangonyx			100851			Lampsilis			8633		Ceriodaphnia			9790		Hyalella			14192		Brachionus			21162
	Site 4		50		100		9		75428737034162		27640367912592		1400		600			Daphnia			26246		Ceriodaphnia			57726		Stenocypris			80480		Crangonyx			95899			Lampsilis			8209		Ceriodaphnia			9309		Hyalella			13495		Brachionus			20123
	Site 5		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 6		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 7		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 8		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 9		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 10		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 11		50		25		85		75428737034162		63245275664384		3200		1400			Daphnia			60055		Ceriodaphnia			132086		Stenocypris			184149		Crangonyx			219432			Lampsilis			18783		Ceriodaphnia			21301		Hyalella			30879		Brachionus			46045
	Site 12		50		50		85		75428737034162		61388044609926		3100		1300			Daphnia			58291		Ceriodaphnia			128208		Stenocypris			178742		Crangonyx			212988			Lampsilis			18232		Ceriodaphnia			20676		Hyalella			29972		Brachionus			44693
	Site 13		50		75		85		75428737034162		60327020429132		3000		1300			Daphnia			57284		Ceriodaphnia			125992		Stenocypris			175652		Crangonyx			209307			Lampsilis			17917		Ceriodaphnia			20318		Hyalella			29454		Brachionus			43920
	Site 14		50		100		85		75428737034162		59585352130146		3000		1300			Daphnia			56580		Ceriodaphnia			124443		Stenocypris			173493		Crangonyx			206734			Lampsilis			17696		Ceriodaphnia			20068		Hyalella			29092		Salmo			41264
	Site 15		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 16		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 17		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 18		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 19		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 20		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370

	Appendix C Acceptable Chronic Toxicity Data of Aluminum to Freshwater Aquatic Animals
									MLR equation
	Species		Hardness (mgL as CaCO3)		pH		DOC(mgL)		EC20 Endpoint			EC20(microgL)			Normalized Conc- EC20(microgL)			SMCV-EC20(microgL)		GMCV-EC20(microgL)			Genus			DOC Notes		Dilution water
	Oligochaete Aeolosoma sp		48		6		025		Reproduction (population size)			1235			17098			17098		17098			Aeolosoma		Invert		Author reported half the detection limit
	Rotifer Brachionus calyciflorus		100		62		025		Reproduction (population size)			431			2555			2555		2555			Brachionus		Invert		Author reported half the detection limit
	Great pond snail Lymnaea stagnalis		117		61		025		Biomass			746			4877			4877		4877			Lymnaea		Mollusk		Author reported half the detection limit
	Fatmucket Lampsilis siliquoidea		1055		604		040		Biomass			169			1042			1042		1042			Lampsilis		Mollusk		Author reported
	Cladoceran Ceriodaphnia dubia		50		715		11		Reproduction - youngstarting adult			1780			1898						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		26		73		05		Reproduction - youngfemale			1557			2718						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		46		75		05		Reproduction - youngfemale			8087			1102						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		96		79		05		Reproduction - youngfemale			6519			796						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		194		81		05		Reproduction - youngfemale			6836			888						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		255		703		05		Reproduction (youngfemale)			250			563						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		122		713		05		Reproduction (youngfemale)			860			1061						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		25		798		05		Reproduction (youngfemale)			700			1006						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		61		803		05		Reproduction (youngfemale)			1010			1363						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		122		810		05		Reproduction (youngfemale)			870			1164						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		255		633		05		Reproduction (youngfemale)			260			1758						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		121		636		05		Reproduction (youngfemale)			390			1101			1182		1182			Ceriodaphnia		Invert		Author reported
	Amphipod Hyalella azteca		95		61		051		Biomass (28 d)			1993			1014						Hyalella			Author reported
	Amphipod Hyalella azteca		106		604		033		Biomass (28 d)			425			2895			1713		1713			Hyalella		Invert		Author reported
	Midge Chironomus riparius		118		558		18		Adult midge emergence			2955			1192						Chironomus			Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Midge Chironomus riparius		119		505		18		Adult midge emergence			8442			22578						Chironomus			Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Midge Chironomus riparius		91		66		051		Reproduction ( of eggscase)			3387			7664			5908		5908			Chironomus		Invert		Author reported
	Atlantic salmon Salmo salar		127		57		18		Biomass			6156			5085			5085		5085			Salmo		Fish		Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Brook trout Salvelinus fontinalis		123		655		19		Biomass			1644			270						Salvelinus			Author reported		wellRO mix
	Brook trout Salvelinus fontinalis		128		565		18		Biomass			1435			1295			5914		5914			Salvelinus		Fish		Author reported		wellRO mix
	Fathead minnow Pimephales promelas		220		770		16		Biomass			6194			3569						Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		well water
	Fathead minnow Pimephales promelas		96		620		025		Fry survival			4286			1734			2488		2488			Pimephales		Fish		Author reported half the detection limit
	Zebrafish Danio rerio		83		61		025		Biomass			2344			1102			1102		1102			Danio		Fish		Author reported half the detection limit
	Wood frog Rana sylvatica		115		469		16		MATC-survival		gt		2000		gt		9746		gt		9746		9746		gt		Rana		Amphib - Other Data		Value is from 2007 FW Cu AWQC Appendix C recommendation		aged tap water

	Appendix A Acceptable Acute Toxicity Data of Aluminum to Freshwater Aquatic Animals
	Species		Methoda		Hardness (mgL as CaCO3)		pH		DOC(mgL)			LC50 or EC50 Conc (microgL)		Reference			Normalized Conc - LC50(microgL)			Species Mean Acute Value(microgL)		Genus Mean Acute Value(microgL)			Genus			DOC Note		Dilution water
	Snail Physa sp		S M T		474		659		11		gt		23400		Call 1984 Call et al 1984		gt		48425			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		755		11			30600		Call 1984 Call et al 1984			26986			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		817		11		gt		24700		Call 1984 Call et al 1984		gt		24040			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		746		11			55500		Call 1984 Call et al 1984			50338			35462		35462			Physa		Mollusk		Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Fatmucket Lampsilis siliquoidea		F M T		1060		612		048		gt		6302		Wang et al 2016 Wang et al 2017		gt		29834		gt		29834		29834		gt		Lampsilis		Mollusk		Author reported
	Cladoceran Ceriodaphnia dubia		S M A		500		742		11			1900		McCauley et al 1986			1728			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		S M A		505		786		11			1500		McCauley et al 1986			1294			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		UW-S Lake Superior lab water
	Cladoceran Ceriodaphnia dubia		S M A		500		813		11			2560		McCauley et al 1986			2431			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		R M T		26		75		05			720		ENSR 1992d			1113			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		R M T		46		76		05			1880		ENSR 1992d			2495			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		R M T		96		78		05			2450		ENSR 1992d			2894			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		S U NR		90		715		05			3727		Fort and Stover 1995			5002			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U NR		90		715		05			5673		Fort and Stover 1995			7613			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U NR		89		76		05			2880		Soucek et al 2001			3362			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		601		05			7112		European Al Association 2009			1691			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		605		2			6865		European Al Association 2009			7088			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		609		4			15581		European Al Association 2009			10072			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		601		05			681		European Al Association 2009			1620			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		603		05			1630		European Al Association 2009			3674			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		597		05			1785		European Al Association 2009			4732			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		592		05			1410		European Al Association 2009			4295			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		699		05		gt		1300		European Al Association 2009		gt		4189			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		785		05		gt		5000		European Al Association 2009		gt		7960			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		68		2		gt		10000		European Al Association 2009		gt		20755			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		782		2		gt		15000		European Al Association 2009		gt		11626			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		677		4		gt		10000		European Al Association 2009		gt		15161			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		766		4		gt		15000		European Al Association 2009		gt		8608			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		79		05		gt		2000		European Al Association 2009		gt		3150			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		789		05		gt		2000		European Al Association 2009		gt		3156			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		604		05			1108		European Al Association 2009			850			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		598		2			11371		European Al Association 2009			4817			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		573		4			80467		European Al Association 2009			43570			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		671		05		gt		10000		European Al Association 2009		gt		23786			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		783		05		gt		5000		European Al Association 2009		gt		6344			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		679		2		gt		10000		European Al Association 2009		gt		10394			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		767		2		gt		15000		European Al Association 2009		gt		9082			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		668		4		gt		15000		European Al Association 2009		gt		12461			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		762		4		gt		15000		European Al Association 2009		gt		6332			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		606		2			33868		European Al Association 2009			7920			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		56		4			104842		European Al Association 2009			48800			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		693		05		gt		5000		European Al Association 2009		gt		7126			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		120		788		05		gt		5000		European Al Association 2009		gt		5898			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		120		676		2		gt		15000		European Al Association 2009		gt		12117			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		771		2		gt		15000		European Al Association 2009		gt		8096			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		66		4		gt		15000		European Al Association 2009		gt		10139			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		76		4		gt		15000		European Al Association 2009		gt		5545			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			11971		European Al Association 2010			2699			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			27478		European Al Association 2010			6194			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			11998		European Al Association 2010			2705			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		607		05			92495		European Al Association 2010			1876			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		609		05			31337		European Al Association 2010			6035			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		61		05			33235		European Al Association 2010			6238			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		708		05		gt		8864		European Al Association 2010		gt		2534			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		779		05		gt		42783		European Al Association 2010		gt		6930			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		753		05			13204		European Al Association 2010			244			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		600		601		05			46326		European Al Association 2010			3798			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		600		599		05		gt		8590		European Al Association 2010		gt		7366			5094				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia reticulata		F M T		451		60		11			304		Shephard 1983			2009			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia reticulata		F M T		40		55		11			362		Shephard 1983			55307			10542		7328			Ceriodaphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake SuperiorDI Mix
	Cladoceran Daphnia magna		S U NR		453		774		11			3900		Biesinger and Christensen 1972			3382			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Daphnia magna		S M T		220		76		16			38200		Kimball 1978			20303			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Well water
	Cladoceran Daphnia magna		S U T		451		725		11			2800		Shephard 1983			2868			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Daphnia magna		S U T		168		599		05			5000		European Al Association 2009		gt		2265			-				Daphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Daphnia magna		S U T		168		792		05			7950		European Al Association 2009			920			3332		3332			Daphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Ostracod Stenocypris major		R M T		1563		651		16			310196		Shuhaimi-Othman et al 2011a			10216			10216		10216			Stenocypris		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (Malaysia)
	Amphipod Crangonyx pseudogracilis		R U T		50		675		16			9190		Martin and Holdich 1986			12174			12174		12174			Crangonyx		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (UK)
	Amphipod Hyalella azteca		F M T		105		613		048		gt		5997		Wang et al 2016 Wang et al 2017		gt		28002		gt		28002		28002		gt		Hyalella		Invert		Author reported
	Stonefly Acroneuria sp		S M T		474		746		11		gt		22600		Call et al 1984		gt		20498		gt		20498		20498		gt		Acroneuria		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Midge Chironomus plumosus		S U T		80		70		16			30000		Fargasova 2001			25361			25361		25361			Chironomus		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (Slovakia)
	Midge Paratanytarsus dissimilis		S M T		1743		728		28		gt		77700		Lamb and Bailey 1981 1983		gt		62318		gt		62318		62318		gt		Paratanytarsus		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Liberty Lake Washington
	Rainbow trout Oncorhynchus mykiss		F M T		274		758		05		gt		9840		Gundersen et al 1994		gt		5745			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		452		762		05		gt		8070		Gundersen et al 1994		gt		5212			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		895		758		05		gt		8160		Gundersen et al 1994		gt		6807			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		1304		758		05		gt		8200		Gundersen et al 1994		gt		7662			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		232		825		05			6170		Gundersen et al 1994			1190			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		350		826		05			6170		Gundersen et al 1994			1523			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		836		829		05			7670		Gundersen et al 1994			3205			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		1158		830		05			6930		Gundersen et al 1994			3554			3661		3661			Oncorhynchus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Atlantic salmon Salmo salar		S U T		68		55		05			584		Hamilton and Haines 1995			21042			-				Salmo			Value is from 2007 FW Cu AWQC Appendix C recommendation		Soft recon
	Atlantic salmon Salmo salar		S U T		68		65		05			599		Hamilton and Haines 1995			2430			7151		7151			Salmo		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Soft recon
	Brook trout Salvelinus fontinalis		S U T		40		56		16			6530		Tandjung 1982			31767			-				Salvelinus			Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Brook trout Salvelinus fontinalis		S U T		18		56		16			3400		Tandjung 1982			28391			-				Salvelinus			Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Brook trout Salvelinus fontinalis		S U T		2		56		16			370		Tandjung 1982			13663			23097		23097			Salvelinus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Green sunfish Lepomis cyanellus		S M T		474		755		11		gt		50000		Call et al 1984		gt		24028		gt		24028		24028		gt		Lepomis		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Rio Grande silvery minnow Hybognathus amarus		R M T		140		81		05		gt		59100		Buhl 2002		gt		39414		gt		39414		39414		gt		Hybognathus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon water
	Fathead minnow Pimephales promelas		S M T		474		761		11		gt		48200		Call et al 1984		gt		21522			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Fathead minnow Pimephales promelas		S M T		474		805		11		gt		49800		Call et al 1984		gt		12972			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Fathead minnow Pimephales promelas		R M T		26		78		05			1160		ENSR 1992c			477			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		46		76		05			8180		ENSR 1992c			5446			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		96		81		05			20300		ENSR 1992c			10966			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		194		81		05			44800		ENSR 1992c			35847			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		140		81		05		gt		59100		Buhl 2002		gt		39414			10168		10168			Pimephales		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon water
	Smallmouth bass Micropterus dolomieui		S M T		1245		505		16			130		Kane and Rabeni 1987			3929			3929		3929			Micropterus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Green tree frog Hyla cinerea		R M T		455		549		05		gt		4052		Jung and Jagoe 1995		gt		20016		gt		20016		20016		gt		Hyla		Amphibian		Value is from 2007 FW Cu AWQC Appendix C recommendation		artificial soft recon

	Normalization Chemistry
	pH		7					FAV		2741
	Hardness		100					CMC		1400
	DOC		1					CCC		390
	Acute Ranked GMAV											Chronic Ranked GMCV
	Rank			GMAV		Genus								Rank			GMCV		Genus
	18		gt		62318		Paratanytarsus		Invert							12			17098		Aeolosoma		Invert
	17		gt		39414		Hybognathus		Fish							11		gt		9746		Rana		Amphib - Other Data
	16			35462		Physa		Mollusk							10			5908		Chironomus		Invert
	15		gt		29834		Lampsilis		Mollusk							9			4877		Lymnaea		Mollusk
	14		gt		28002		Hyalella		Invert							8			2555		Brachionus		Invert
	13			25361		Chironomus		Invert							7			2488		Pimephales		Fish
	12		gt		24028		Lepomis		Fish							6			1713		Hyalella		Invert
	11			23097		Salvelinus		Fish							5			1182		Ceriodaphnia		Invert
	10		gt		20498		Acroneuria		Invert							4			1102		Danio		Fish
	9		gt		20016		Hyla		Amphibian							3			1042		Lampsilis		Mollusk
	8			12174		Crangonyx		Invert							2			591		Salvelinus		Fish
	7			10216		Stenocypris		Invert							1			509		Salmo		Fish
	6			10168		Pimephales		Fish
	5			7328		Ceriodaphnia		Invert
	4			7151		Salmo		Fish
	3			3929		Micropterus		Fish
	2			3661		Oncorhynchus		Fish
	1			3332		Daphnia		Invert
		N		Rank		Genus		GMAV		ln(GMAV)		ln(GMAV)2		P=R(N+1)		sqrt(P)				N		Rank		Genus		GMCV		ln(GMCV)		ln(GMCV)2		P=R(N+1)		sqrt(P)
		18		4		Salmo		7151		887		7876		0211		0459				12		4		Danio		1102		700		4907		0308		0555
			3		Micropterus		3929		828		6849		0158		0397					3		Lampsilis		1042		695		4829		0231		0480
			2		Oncorhynchus		3661		821		6733		0105		0324					2		Salvelinus		591		638		4074		0154		0392
			1		Daphnia		3332		811		6579		0053		0229					1		Salmo		509		623		3883		0077		0277
			Sum				3347		2804		0526		141					Sum				2657		1769		0769		170
			S2 =		1223										S2 =		1080
			L =		7134										L =		5242
			A =		7916										A =		5976
			FAV =		2741										FCV =		3940
			CMC =		1371

				All concentrations reported are microgL total Aluminum
				lt-----Click Button after entering data
	ENTER DATA HERE									ERRORNUM		ACUTE														CHRONIC
										Rank 1				Rank 2				Rank 3				Rank 4					Rank 1				Rank 2				Rank 3				Rank 4
	SiteName		DOC(mgL)		Hardness(mgL as CaCO3)		pH			FAV		CMC		CCC			Genus		GMAV			Genus		GMAV			Genus		GMAV			Genus		GMAV				Genus		GMCV			Genus		GMCV			Genus		GMCV			Genus		GMCV
	Site 1		50		25		9		75428737034162		35229324666008		1800		770			Daphnia			33452		Ceriodaphnia			73576		Stenocypris			102576		Crangonyx			122230			Lampsilis			10463		Ceriodaphnia			11865		Hyalella			17200		Brachionus			25648
	Site 2		50		50		9		75428737034162		31204991509063		1600		680			Daphnia			29631		Ceriodaphnia			65171		Stenocypris			90859		Crangonyx			108267			Lampsilis			9268		Ceriodaphnia			10510		Hyalella			15236		Brachionus			22718
	Site 3		50		75		9		75428737034162		29067527928199		1500		640			Daphnia			27601		Ceriodaphnia			60707		Stenocypris			84635		Crangonyx			100851			Lampsilis			8633		Ceriodaphnia			9790		Hyalella			14192		Brachionus			21162
	Site 4		50		100		9		75428737034162		27640367912592		1400		600			Daphnia			26246		Ceriodaphnia			57726		Stenocypris			80480		Crangonyx			95899			Lampsilis			8209		Ceriodaphnia			9309		Hyalella			13495		Brachionus			20123
	Site 5		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 6		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 7		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 8		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 9		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 10		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 11		50		25		85		75428737034162		63245275664384		3200		1400			Daphnia			60055		Ceriodaphnia			132086		Stenocypris			184149		Crangonyx			219432			Lampsilis			18783		Ceriodaphnia			21301		Hyalella			30879		Brachionus			46045
	Site 12		50		50		85		75428737034162		61388044609926		3100		1300			Daphnia			58291		Ceriodaphnia			128208		Stenocypris			178742		Crangonyx			212988			Lampsilis			18232		Ceriodaphnia			20676		Hyalella			29972		Brachionus			44693
	Site 13		50		75		85		75428737034162		60327020429132		3000		1300			Daphnia			57284		Ceriodaphnia			125992		Stenocypris			175652		Crangonyx			209307			Lampsilis			17917		Ceriodaphnia			20318		Hyalella			29454		Brachionus			43920
	Site 14		50		100		85		75428737034162		59585352130146		3000		1300			Daphnia			56580		Ceriodaphnia			124443		Stenocypris			173493		Crangonyx			206734			Lampsilis			17696		Ceriodaphnia			20068		Hyalella			29092		Salmo			41264
	Site 15		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 16		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 17		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 18		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 19		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 20		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370

Framework for Updated Draft Aluminum Criteria

I Screen toxicological studies for high

quality data

II Consider water conditions effects on

aluminum bioavailability

III Normalize toxicity data in species

sensitivity distribution to a set of water quality conditions

IV Construct sensitivity distribution to determine criteria

at set conditions

9

I Screen toxicological studies for high quality data

bull First step in all criteria developmentndash Data are evaluated based on the data quality objectives

outlined in the 1985 Guidelines methodology and other toxicity testing recommendations (ie EPA ASTM)

ndash High quality data are used quantitatively to develop criteria magnitudes

ndash Lower quality data are used qualitatively if appropriatendash Lowest quality data are considered unacceptable and not

usedbull Data are grouped into acute and chronic exposures

10

Aluminum Toxicity Databull The updated freshwater aluminum criteria meets the

acute and chronic minimum data requirements outlined in EPArsquos 1985 Guidelines

InvertebrateSpecies

Fish Species

AmphibianSpecies

Total Genera

Acute1988 AWQC 8 7 - 142017 Draft AWQC 11 8 1 18

Chronic1988 AWQC 2 1 - 32017 Draft AWQC 7 4 - 11

11 1988 AWQC Chronic CCC based on two studies for the striped

bass and brook trout

Aluminum Toxicity Data Freshwater mussels have been

suggested to be potentially sensitive to aluminum

EPA worked collaboratively with USGSrsquos Columbia Environmental Research Center (CERC) to have acute and chronic aluminum toxicity tests conducted with a unionid mussel (fatmucket Lampsilis siliquoidea) in water-only exposures to obtain additional data on invertebrates New data on amphipod (Hyalella azteca)

were collected to confirm laboratory conditions

12

Aluminum Toxicity Data

13

bull New data and models examinedndash Data submitted to EU REACH program

bull New freshwater aluminum toxicity data generated by Oregon State University

bull 2016 BLM model provided by the European Aluminum Association

ndash Saltwater data provided by Australia bull Insufficient data to develop estuarinemarine acute or chronic

criteria at this timendash Multiple Linear Regression (MLR) model developed by

DeForest et al 2017bull DeForest DK KV Brix LM Tear and WJ Adams 2017 Multiple Linear Regression (MLR) models for predicting chronic aluminum

toxicity to freshwater aquatic organisms and developing water quality guidelines Environ Toxicol Chem (DOI101002etc3922)

14

Minimum Dataset For Freshwater Criteria Derivation

SALMONID SECONDFISHFAMILY

CHORDATA

PLANKTONICCRUSTACEAN

BENTHIC CRUSTACEAN

INSECT ROTIFERA ANNELIDA MOLLUSCA

OTHERINSECT ORMOLLUSCA

EstuarineMarine Minimum Data Requirements

15

Family Minimum Data Requirement EstuarineMarine

Acute(Phylum Family Genus)

Chronic(Phylum Family Genus)

Family in the phylum Chordata - -

Family in the phylum Chordata - -

Either the Mysidae or Penaeidae family - -

Family in a phylum other than Arthropoda or Chordata Mollusca Ostreidae Crassostrea -

Family in a phylum other than Chordata Annelida Nereididae Neanthes -

Family in a phylum other than Chordata Annelida Capitellidae Capitella -

Family in a phylum other than Chordata Annelida Ctenodrilidae Ctenodrilus -

Any other family Arthropoda Ameiridae Nitokra -

II Evaluate water quality conditions to determine applicability

bull Criteria are often based on one or more water quality conditions (eg hardness) that affect toxicity of the material to test organisms

bull Aluminum toxicity is highly dependent on the pH of the test waterndash The pH of test waters affects the amount and form

of aluminum that is bioavailable to test speciesndash Hardness and DOC (Dissolved Organic Carbon)

also influence the amount of aluminum that is bioavailable

16

Multiple Bioavailability Modeling Approaches Underwent External Peer Review

17

bull Biotic Ligand Modelsbull Full aluminum BLM (2016 model version) ndash Temperature pH

Dissolved organic carbon (DOC) Major cations (calcium [Ca] magnesium [Mg] sodium [Na] and potassium [K]) Major anions (sulfate [SO4] and chloride [Cl]) Alkalinity and Sulfide

bull Simplified aluminum BLM - pH hardness DOC temperature

bull Multilinear Regression Equationsbull pH and Hardness

Accounting for Effects of Water Chemistry on Bioavailability and Toxicity

bull Water quality criteria can be modified quantitatively when enough data are available to demonstrate that those conditions similarly affect the results with a variety of speciesndash High quality data in the draft aluminum document demonstrated

that pH DOC and hardness affected a variety of species in a similar way when exposed to aluminum concentrations

bull Recent publications by Cardwell et al (2017) and Gensemer et al (2017) summarized short-term aluminum chronic toxicity data across a range of DOC pH and hardness values for three species

18

Multiple Linear Regression (MLR) Models to Account for Effects of Water Chemistry

bull MLR models were developed for each species (DeForest et al 2017)ndash 119862119862 119889119889119906119906119887119887119894119894119886119886 11986411986411986211986220=119890119890[minus41026+[0525timesln(119863119863119874119874119862119862)]+[2201timesln(ℎ119886119886119903119903119889119889)]+(11282times119901119901119867119867)minus(0633times1199011199011198671198672)minus[0264times119901119901119867119867ln(ℎ119886119886119903119903119889119889)]]

ndash 119875119875 119901119901119903119903119900119900119898119898119890119890119897119897119886119886119904119904 11986411986411986211986220=119890119890[minus14029+[0503timesln(119863119863119874119874119862119862)]+[3443timesln(ℎ119886119886119903119903119889119889)]+(3131times119901119901119867119867)minus[0494times119901119901119867119867ln(ℎ119886119886119903119903119889119889)]]

bull These models were applied to both the acute and chronic aluminum toxicity datandash The C dubia model was applied to invertebrate datandash The P promelas model was applied to vertebrate data

19

III Normalize toxicity data in species sensitivity distribution to a set of water

quality conditionsbull Just like single parameter hardness models the MLR

models were used to normalize the aluminum toxicity data to a single set of water quality conditionsndash As an illustration for one set of calculations the draft

aluminum AWQC set these conditions to pH 7 hardness of 100 mgL and DOC of 1 mgL

bull This allows for comparisons to be made between species based on their sensitivity to aluminumndash Apples to apples

20

IV Construct sensitivity distribution to determine criteria at set conditions

21

Figure 8 Ranked Summary of Total Aluminum Genus Mean Acute Values (GMAVs) - Freshwater at pH 7 Hardness of 100 mgL and DOC of 1 mgL

IV Construct sensitivity distribution to determine criteria at set conditions

22

Figure 10 Ranked Summary of Total Aluminum Genus Mean Chronic Values (GMCVs) ndash Freshwater Supplemented with Other Data to Fulfill Missing MDRs at pH 7 Hardness of 100 mgL and DOC of 1 mgL

Aluminum Criteria Calculatorbull The relative ranking of genera in the sensitivity

distribution to which the toxicity data are normalized will change based on the set of water chemistry conditions (ie pH hardness and DOC)

bull To make calculating aluminum criteria easier EPA developed an Aluminum Criteria Calculatorndash Automated so that user only needs to enter water chemistry

conditions of interestndash Allows quick calculations for a variety of conditionsndash Limits calculations to the recommended conditions for which

the MLR models were developed were specified for hardness and DOC

bull No extrapolation beyond hardness and DOC empirical data bounds

23

Aluminum Criteria Calculator SD Example

24

pH=7 Hardness=100 DOC=1 pH=6 Hardness=25 DOC=1

Chronic Ranked GMCVRank GMCV Genus

12 17098 Aeolosoma Invert11 gt 9746 Rana Amphib - Other Data10 5908 Chironomus Invert

9 4877 Lymnaea Mollusk8 2555 Brachionus Invert7 2488 Pimephales Fish6 1713 Hyalella Invert5 1182 Ceriodaphnia Invert4 1102 Danio Fish3 1042 Lampsilis Mollusk2 591 Salvelinus Fish1 509 Salmo Fish

FCV = 3940 microgL total aluminum

Chronic Ranked GMCVRank GMCV Genus

12 gt 2131 Rana Amphib - Other Data11 1710 Aeolosoma Invert10 591 Chironomus Invert

9 544 Pimephales Fish8 488 Lymnaea Mollusk7 255 Brachionus Invert6 241 Danio Fish5 171 Hyalella Invert4 129 Salvelinus Fish3 118 Ceriodaphnia Invert2 111 Salmo Fish1 104 Lampsilis Mollusk

FCV = 987 microgL total aluminum

Multiple Scenarios

Run Scenarios

Summary Sheet

Acute Dataset

Chronic Dataset

Multiple Scenarios

Run Scenarios

Summary Sheet

Acute Dataset

Chronic Dataset

Boundaries of the Criteria Outputsbull MLR models were developed using data that

encompassndash DOC range of 008-5 mgLndash Hardness range of 98-127 mgL (as CaCO3)ndash pH range of 6-81

bull MLR criteria outputs are bounded atndash Maximum of 150 mgL total hardness (as CaCO3)

ndash DOC of 50 mgLndash pH range of the model is 50 to 90

25

Aluminum Criteria Look-Up Tables

26

bull EPA also provided criteria look-up tables in Appendix K

Har

dnes

s

CCC(microgL total aluminum)

(DOC=05 mgL)

pH 50 pH 55 pH 60 pH 65 pH 70 pH 75 pH 80 pH 85 pH 90

25 23 a 14 b 69 c 160 f 350 f 620 i 540 b 410 a 230 a

50 43 a 24 b 100 d 170 f 310 f 630 f 670 j 400 a 200 a

75 61 a 33 b 130 e 180 f 290 f 530 f 730 i 390 a 190 a

100 78 a 41 b 150 e 190 f 280 g 460 f 770 c 390 a 180 a

150 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

200 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

250 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

300 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

350 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

400 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

Aluminum Criteria Look-Up Tables

27

bull EPA also provided criteria look-up tables in Appendix K

Har

dnes

s

CCC(microgL total aluminum)

(DOC=10 mgL)

pH 50 pH 55 pH 60 pH 65 pH 70 pH 75 pH 80 pH 85 pH 90

25 33 a 21 b 99 c 220 f 490 f 900 i 780 b 590 a 330 a

50 61 a 35 b 150 e 250 f 440 f 890 f 960 j 580 a 290 a

75 88 a 47 b 180 e 260 f 410 f 740 f 1100 i 570 a 270 a

100 11 a 59 b 210 e 270 f 390 g 650 f 1100 c 560 b 260 a

150 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

200 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

250 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

300 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

350 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

400 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

Aluminum Criteria Look-Up Tables

28

bull EPA also provided criteria look-up tables in Appendix K

Har

dnes

s

CCC(microgL total aluminum)

(DOC=25 mgL)

pH 50 pH 55 pH 60 pH 65 pH 70 pH 75 pH 80 pH 85 pH 90

25 54 a 34 b 160 c 350 f 770 f 1500 i 1300 b 960 a 540 a

50 99 a 57 b 230 e 390 f 690 f 1400 f 1600 j 930 a 470 a

75 14 a 77 b 290 e 410 f 640 f 1200 f 1700 i 920 a 440 a

100 18 a 96 b 330 e 420 f 620 g 1000 f 1800 d 920 b 420 a

150 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

200 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

250 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

300 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

350 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

400 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

Aluminum Criteria Look-Up Tables

29

bull EPA also provided criteria look-up tables in Appendix K

Har

dnes

s

CCC(microgL total aluminum)

(DOC=50 mgL)

pH 50 pH 55 pH 60 pH 65 pH 70 pH 75 pH 80 pH 85 pH 90

25 77 a 49 b 230 c 500 f 1100 f 2100 c 1900 b 1400 a 770 a

50 14 a 82 b 330 e 540 f 970 f 2000 f 2200 j 1300 a 680 a

75 20 a 110 b 410 e 570 f 900 f 1600 f 2500 i 1300 a 640 a

100 26 a 140 b 460 f 590 f 880 g 1400 f 2500 d 1300 b 600 a

150 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

200 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

250 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

300 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

350 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

400 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

Status

30

bull Draft Document Released to the Federal Register on 72817ndash Public comment period of 60 days ndash Due Date for Comments is 92617

bull Revisions will be made considering public commentsbull Subsequent EPA intra-agency review of final criteria

document will then be conducted bull Publication of final criteria document planned for Late Fall

2017

Federal Register Webpagehttpswwwfederalregistergovdocuments201

707282017-15968request-for-scientific-views-draft-updated-aquatic-life-ambient-water-quality-criteria-for-aluminum

Aluminum Criteria Webpagehttpswwwepagovwqc2017-draft-aquatic-life-

criteria-aluminum-freshwater-documents

31

	Appendix C Acceptable Chronic Toxicity Data of Aluminum to Freshwater Aquatic Animals
									MLR equation
	Species		Hardness (mgL as CaCO3)		pH		DOC(mgL)		EC20 Endpoint			EC20(microgL)			Normalized Conc- EC20(microgL)			SMCV-EC20(microgL)		GMCV-EC20(microgL)			Genus			DOC Notes		Dilution water
	Oligochaete Aeolosoma sp		48		6		025		Reproduction (population size)			1235			1710			1710		1710			Aeolosoma		Invert		Author reported half the detection limit
	Rotifer Brachionus calyciflorus		100		62		025		Reproduction (population size)			431			255			255		255			Brachionus		Invert		Author reported half the detection limit
	Great pond snail Lymnaea stagnalis		117		61		025		Biomass			746			488			488		488			Lymnaea		Mollusk		Author reported half the detection limit
	Fatmucket Lampsilis siliquoidea		1055		604		040		Biomass			169			104			104		104			Lampsilis		Mollusk		Author reported
	Cladoceran Ceriodaphnia dubia		50		715		11		Reproduction - youngstarting adult			1780			190						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		26		73		05		Reproduction - youngfemale			1557			272						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		46		75		05		Reproduction - youngfemale			8087			110						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		96		79		05		Reproduction - youngfemale			6519			80						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		194		81		05		Reproduction - youngfemale			6836			89						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		255		703		05		Reproduction (youngfemale)			250			56						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		122		713		05		Reproduction (youngfemale)			860			106						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		25		798		05		Reproduction (youngfemale)			700			101						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		61		803		05		Reproduction (youngfemale)			1010			136						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		122		810		05		Reproduction (youngfemale)			870			116						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		255		633		05		Reproduction (youngfemale)			260			176						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		121		636		05		Reproduction (youngfemale)			390			110			118		118			Ceriodaphnia		Invert		Author reported
	Amphipod Hyalella azteca		95		61		051		Biomass (28 d)			1993			101						Hyalella			Author reported
	Amphipod Hyalella azteca		106		604		033		Biomass (28 d)			425			289			171		171			Hyalella		Invert		Author reported
	Midge Chironomus riparius		118		558		18		Adult midge emergence			2955			119						Chironomus			Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Midge Chironomus riparius		119		505		18		Adult midge emergence			8442			2258						Chironomus			Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Midge Chironomus riparius		91		66		051		Reproduction ( of eggscase)			3387			766			591		591			Chironomus		Invert		Author reported
	Atlantic salmon Salmo salar		127		57		18		Biomass			6156			1112			1112		1112			Salmo		Fish		Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Brook trout Salvelinus fontinalis		123		655		19		Biomass			1644			59						Salvelinus			Author reported		wellRO mix
	Brook trout Salvelinus fontinalis		128		565		18		Biomass			1435			283			1293		1293			Salvelinus		Fish		Author reported		wellRO mix
	Fathead minnow Pimephales promelas		220		770		16		Biomass			6194			781						Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		well water
	Fathead minnow Pimephales promelas		96		620		025		Fry survival			4286			379			544		544			Pimephales		Fish		Author reported half the detection limit
	Zebrafish Danio rerio		83		61		025		Biomass			2344			241			241		241			Danio		Fish		Author reported half the detection limit
	Wood frog Rana sylvatica		115		469		16		MATC-survival		gt		2000		gt		2131		gt		2131		2131		gt		Rana		Amphib - Other Data		Value is from 2007 FW Cu AWQC Appendix C recommendation		aged tap water

	Appendix A Acceptable Acute Toxicity Data of Aluminum to Freshwater Aquatic Animals
	Species		Methoda		Hardness (mgL as CaCO3)		pH		DOC(mgL)			LC50 or EC50 Conc (microgL)		Reference			Normalized Conc - LC50(microgL)			Species Mean Acute Value(microgL)		Genus Mean Acute Value(microgL)			Genus			DOC Note		Dilution water
	Snail Physa sp		S M T		474		659		11		gt		23400		Call 1984 Call et al 1984		gt		4843			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		755		11			30600		Call 1984 Call et al 1984			2699			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		817		11		gt		24700		Call 1984 Call et al 1984		gt		2404			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		746		11			55500		Call 1984 Call et al 1984			5034			3546		3546			Physa		Mollusk		Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Fatmucket Lampsilis siliquoidea		F M T		1060		612		048		gt		6302		Wang et al 2016 Wang et al 2017		gt		2983		gt		2983		2983		gt		Lampsilis		Mollusk		Author reported
	Cladoceran Ceriodaphnia dubia		S M A		500		742		11			1900		McCauley et al 1986			173			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		S M A		505		786		11			1500		McCauley et al 1986			129			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		UW-S Lake Superior lab water
	Cladoceran Ceriodaphnia dubia		S M A		500		813		11			2560		McCauley et al 1986			243			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		R M T		26		75		05			720		ENSR 1992d			111			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		R M T		46		76		05			1880		ENSR 1992d			250			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		R M T		96		78		05			2450		ENSR 1992d			289			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		S U NR		90		715		05			3727		Fort and Stover 1995			500			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U NR		90		715		05			5673		Fort and Stover 1995			761			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U NR		89		76		05			2880		Soucek et al 2001			336			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		601		05			7112		European Al Association 2009			169			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		605		2			6865		European Al Association 2009			709			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		609		4			15581		European Al Association 2009			1007			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		601		05			681		European Al Association 2009			162			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		603		05			1630		European Al Association 2009			367			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		597		05			1785		European Al Association 2009			473			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		592		05			1410		European Al Association 2009			429			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		699		05		gt		1300		European Al Association 2009		gt		419			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		785		05		gt		5000		European Al Association 2009		gt		796			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		68		2		gt		10000		European Al Association 2009		gt		2076			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		782		2		gt		15000		European Al Association 2009		gt		1163			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		677		4		gt		10000		European Al Association 2009		gt		1516			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		766		4		gt		15000		European Al Association 2009		gt		861			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		79		05		gt		2000		European Al Association 2009		gt		315			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		789		05		gt		2000		European Al Association 2009		gt		316			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		604		05			1108		European Al Association 2009			85			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		598		2			11371		European Al Association 2009			482			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		573		4			80467		European Al Association 2009			4357			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		671		05		gt		10000		European Al Association 2009		gt		2379			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		783		05		gt		5000		European Al Association 2009		gt		634			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		679		2		gt		10000		European Al Association 2009		gt		1039			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		767		2		gt		15000		European Al Association 2009		gt		908			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		668		4		gt		15000		European Al Association 2009		gt		1246			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		762		4		gt		15000		European Al Association 2009		gt		633			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		606		2			33868		European Al Association 2009			792			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		56		4			104842		European Al Association 2009			4880			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		693		05		gt		5000		European Al Association 2009		gt		713			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		120		788		05		gt		5000		European Al Association 2009		gt		590			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		120		676		2		gt		15000		European Al Association 2009		gt		1212			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		771		2		gt		15000		European Al Association 2009		gt		810			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		66		4		gt		15000		European Al Association 2009		gt		1014			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		76		4		gt		15000		European Al Association 2009		gt		555			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			11971		European Al Association 2010			270			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			27478		European Al Association 2010			619			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			11998		European Al Association 2010			270			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		607		05			92495		European Al Association 2010			188			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		609		05			31337		European Al Association 2010			604			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		61		05			33235		European Al Association 2010			624			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		708		05		gt		8864		European Al Association 2010		gt		253			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		779		05		gt		42783		European Al Association 2010		gt		693			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		753		05			13204		European Al Association 2010			24			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		600		601		05			46326		European Al Association 2010			380			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		600		599		05		gt		8590		European Al Association 2010		gt		737			509				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia reticulata		F M T		451		60		11			304		Shephard 1983			201			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia reticulata		F M T		40		55		11			362		Shephard 1983			5531			1054		733			Ceriodaphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake SuperiorDI Mix
	Cladoceran Daphnia magna		S U NR		453		774		11			3900		Biesinger and Christensen 1972			338			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Daphnia magna		S M T		220		76		16			38200		Kimball 1978			2030			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Well water
	Cladoceran Daphnia magna		S U T		451		725		11			2800		Shephard 1983			287			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Daphnia magna		S U T		168		599		05			5000		European Al Association 2009		gt		227			-				Daphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Daphnia magna		S U T		168		792		05			7950		European Al Association 2009			92			333		333			Daphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Ostracod Stenocypris major		R M T		1563		651		16			310196		Shuhaimi-Othman et al 2011a			1022			1022		1022			Stenocypris		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (Malaysia)
	Amphipod Crangonyx pseudogracilis		R U T		50		675		16			9190		Martin and Holdich 1986			1217			1217		1217			Crangonyx		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (UK)
	Amphipod Hyalella azteca		F M T		105		613		048		gt		5997		Wang et al 2016 Wang et al 2017		gt		2800		gt		2800		2800		gt		Hyalella		Invert		Author reported
	Stonefly Acroneuria sp		S M T		474		746		11		gt		22600		Call et al 1984		gt		2050		gt		2050		2050		gt		Acroneuria		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Midge Chironomus plumosus		S U T		80		70		16			30000		Fargasova 2001			2536			2536		2536			Chironomus		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (Slovakia)
	Midge Paratanytarsus dissimilis		S M T		1743		728		28		gt		77700		Lamb and Bailey 1981 1983		gt		6232		gt		6232		6232		gt		Paratanytarsus		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Liberty Lake Washington
	Rainbow trout Oncorhynchus mykiss		F M T		274		758		05		gt		9840		Gundersen et al 1994		gt		1256			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		452		762		05		gt		8070		Gundersen et al 1994		gt		1140			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		895		758		05		gt		8160		Gundersen et al 1994		gt		1489			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		1304		758		05		gt		8200		Gundersen et al 1994		gt		1676			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		232		825		05			6170		Gundersen et al 1994			260			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		350		826		05			6170		Gundersen et al 1994			333			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		836		829		05			7670		Gundersen et al 1994			701			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		1158		830		05			6930		Gundersen et al 1994			777			801		801			Oncorhynchus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Atlantic salmon Salmo salar		S U T		68		55		05			584		Hamilton and Haines 1995			4602			-				Salmo			Value is from 2007 FW Cu AWQC Appendix C recommendation		Soft recon
	Atlantic salmon Salmo salar		S U T		68		65		05			599		Hamilton and Haines 1995			531			1564		1564			Salmo		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Soft recon
	Brook trout Salvelinus fontinalis		S U T		40		56		16			6530		Tandjung 1982			6947			-				Salvelinus			Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Brook trout Salvelinus fontinalis		S U T		18		56		16			3400		Tandjung 1982			6209			-				Salvelinus			Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Brook trout Salvelinus fontinalis		S U T		2		56		16			370		Tandjung 1982			2988			5051		5051			Salvelinus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Green sunfish Lepomis cyanellus		S M T		474		755		11		gt		50000		Call et al 1984		gt		5255		gt		5255		5255		gt		Lepomis		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Rio Grande silvery minnow Hybognathus amarus		R M T		140		81		05		gt		59100		Buhl 2002		gt		8620		gt		8620		8620		gt		Hybognathus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon water
	Fathead minnow Pimephales promelas		S M T		474		761		11		gt		48200		Call et al 1984		gt		4707			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Fathead minnow Pimephales promelas		S M T		474		805		11		gt		49800		Call et al 1984		gt		2837			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Fathead minnow Pimephales promelas		R M T		26		78		05			1160		ENSR 1992c			104			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		46		76		05			8180		ENSR 1992c			1191			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		96		81		05			20300		ENSR 1992c			2398			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		194		81		05			44800		ENSR 1992c			7839			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		140		81		05		gt		59100		Buhl 2002		gt		8620			2224		2224			Pimephales		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon water
	Smallmouth bass Micropterus dolomieui		S M T		1245		505		16			130		Kane and Rabeni 1987			859			859		859			Micropterus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Green tree frog Hyla cinerea		R M T		455		549		05		gt		4052		Jung and Jagoe 1995		gt		4377		gt		4377		4377		gt		Hyla		Amphibian		Value is from 2007 FW Cu AWQC Appendix C recommendation		artificial soft recon

	Normalization Chemistry
	pH		6					FAV		360
	Hardness		25					CMC		180
	DOC		1					CCC		99
	Acute Ranked GMAV											Chronic Ranked GMCV
	Rank			GMAV		Genus								Rank			GMCV		Genus
	18		gt		8620		Hybognathus		Fish							12		gt		2131		Rana		Amphib - Other Data
	17		gt		6232		Paratanytarsus		Invert							11			1710		Aeolosoma		Invert
	16		gt		5255		Lepomis		Fish							10			591		Chironomus		Invert
	15			5051		Salvelinus		Fish							9			544		Pimephales		Fish
	14		gt		4377		Hyla		Amphibian							8			488		Lymnaea		Mollusk
	13			3546		Physa		Mollusk							7			255		Brachionus		Invert
	12		gt		2983		Lampsilis		Mollusk							6			241		Danio		Fish
	11		gt		2800		Hyalella		Invert							5			171		Hyalella		Invert
	10			2536		Chironomus		Invert							4			129		Salvelinus		Fish
	9			2224		Pimephales		Fish							3			118		Ceriodaphnia		Invert
	8		gt		2050		Acroneuria		Invert							2			111		Salmo		Fish
	7			1564		Salmo		Fish							1			104		Lampsilis		Mollusk
	6			1217		Crangonyx		Invert
	5			1022		Stenocypris		Invert
	4			859		Micropterus		Fish
	3			801		Oncorhynchus		Fish
	2			733		Ceriodaphnia		Invert
	1			333		Daphnia		Invert
		N		Rank		Genus		GMAV		ln(GMAV)		ln(GMAV)2		P=R(N+1)		sqrt(P)				N		Rank		Genus		GMCV		ln(GMCV)		ln(GMCV)2		P=R(N+1)		sqrt(P)
		18		4		Micropterus		859		676		4564		0211		0459				12		4		Salvelinus		129		486		2364		0308		0555
			3		Oncorhynchus		801		669		4469		0158		0397					3		Ceriodaphnia		118		477		2277		0231		0480
			2		Ceriodaphnia		733		660		4352		0105		0324					2		Salmo		111		471		2220		0154		0392
			1		Daphnia		333		581		3374		0053		0229					1		Lampsilis		104		465		2159		0077		0277
			Sum				2585		1676		0526		141					Sum				1899		902		0769		170
			S2 =		1987										S2 =		059
			L =		4890										L =		4420
			A =		5887										A =		4592
			FAV =		360										FCV =		987
			CMC =		180

				All concentrations reported are microgL total Aluminum
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	ENTER DATA HERE									ERRORNUM		ACUTE														CHRONIC
										Rank 1				Rank 2				Rank 3				Rank 4					Rank 1				Rank 2				Rank 3				Rank 4
	SiteName		DOC(mgL)		Hardness(mgL as CaCO3)		pH			FAV		CMC		CCC			Genus		GMAV			Genus		GMAV			Genus		GMAV			Genus		GMAV				Genus		GMCV			Genus		GMCV			Genus		GMCV			Genus		GMCV
	Site 1		50		25		9		75428737034162		35229324666008		1800		770			Daphnia			33452		Ceriodaphnia			73576		Stenocypris			102576		Crangonyx			122230			Lampsilis			10463		Ceriodaphnia			11865		Hyalella			17200		Brachionus			25648
	Site 2		50		50		9		75428737034162		31204991509063		1600		680			Daphnia			29631		Ceriodaphnia			65171		Stenocypris			90859		Crangonyx			108267			Lampsilis			9268		Ceriodaphnia			10510		Hyalella			15236		Brachionus			22718
	Site 3		50		75		9		75428737034162		29067527928199		1500		640			Daphnia			27601		Ceriodaphnia			60707		Stenocypris			84635		Crangonyx			100851			Lampsilis			8633		Ceriodaphnia			9790		Hyalella			14192		Brachionus			21162
	Site 4		50		100		9		75428737034162		27640367912592		1400		600			Daphnia			26246		Ceriodaphnia			57726		Stenocypris			80480		Crangonyx			95899			Lampsilis			8209		Ceriodaphnia			9309		Hyalella			13495		Brachionus			20123
	Site 5		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 6		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 7		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 8		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 9		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 10		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 11		50		25		85		75428737034162		63245275664384		3200		1400			Daphnia			60055		Ceriodaphnia			132086		Stenocypris			184149		Crangonyx			219432			Lampsilis			18783		Ceriodaphnia			21301		Hyalella			30879		Brachionus			46045
	Site 12		50		50		85		75428737034162		61388044609926		3100		1300			Daphnia			58291		Ceriodaphnia			128208		Stenocypris			178742		Crangonyx			212988			Lampsilis			18232		Ceriodaphnia			20676		Hyalella			29972		Brachionus			44693
	Site 13		50		75		85		75428737034162		60327020429132		3000		1300			Daphnia			57284		Ceriodaphnia			125992		Stenocypris			175652		Crangonyx			209307			Lampsilis			17917		Ceriodaphnia			20318		Hyalella			29454		Brachionus			43920
	Site 14		50		100		85		75428737034162		59585352130146		3000		1300			Daphnia			56580		Ceriodaphnia			124443		Stenocypris			173493		Crangonyx			206734			Lampsilis			17696		Ceriodaphnia			20068		Hyalella			29092		Salmo			41264
	Site 15		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 16		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 17		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 18		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 19		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 20		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370

	Appendix C Acceptable Chronic Toxicity Data of Aluminum to Freshwater Aquatic Animals
									MLR equation
	Species		Hardness (mgL as CaCO3)		pH		DOC(mgL)		EC20 Endpoint			EC20(microgL)			Normalized Conc- EC20(microgL)			SMCV-EC20(microgL)		GMCV-EC20(microgL)			Genus			DOC Notes		Dilution water
	Oligochaete Aeolosoma sp		48		6		025		Reproduction (population size)			1235			17098			17098		17098			Aeolosoma		Invert		Author reported half the detection limit
	Rotifer Brachionus calyciflorus		100		62		025		Reproduction (population size)			431			2555			2555		2555			Brachionus		Invert		Author reported half the detection limit
	Great pond snail Lymnaea stagnalis		117		61		025		Biomass			746			4877			4877		4877			Lymnaea		Mollusk		Author reported half the detection limit
	Fatmucket Lampsilis siliquoidea		1055		604		040		Biomass			169			1042			1042		1042			Lampsilis		Mollusk		Author reported
	Cladoceran Ceriodaphnia dubia		50		715		11		Reproduction - youngstarting adult			1780			1898						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		26		73		05		Reproduction - youngfemale			1557			2718						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		46		75		05		Reproduction - youngfemale			8087			1102						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		96		79		05		Reproduction - youngfemale			6519			796						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		194		81		05		Reproduction - youngfemale			6836			888						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		255		703		05		Reproduction (youngfemale)			250			563						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		122		713		05		Reproduction (youngfemale)			860			1061						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		25		798		05		Reproduction (youngfemale)			700			1006						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		61		803		05		Reproduction (youngfemale)			1010			1363						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		122		810		05		Reproduction (youngfemale)			870			1164						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		255		633		05		Reproduction (youngfemale)			260			1758						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		121		636		05		Reproduction (youngfemale)			390			1101			1182		1182			Ceriodaphnia		Invert		Author reported
	Amphipod Hyalella azteca		95		61		051		Biomass (28 d)			1993			1014						Hyalella			Author reported
	Amphipod Hyalella azteca		106		604		033		Biomass (28 d)			425			2895			1713		1713			Hyalella		Invert		Author reported
	Midge Chironomus riparius		118		558		18		Adult midge emergence			2955			1192						Chironomus			Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Midge Chironomus riparius		119		505		18		Adult midge emergence			8442			22578						Chironomus			Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Midge Chironomus riparius		91		66		051		Reproduction ( of eggscase)			3387			7664			5908		5908			Chironomus		Invert		Author reported
	Atlantic salmon Salmo salar		127		57		18		Biomass			6156			5085			5085		5085			Salmo		Fish		Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Brook trout Salvelinus fontinalis		123		655		19		Biomass			1644			270						Salvelinus			Author reported		wellRO mix
	Brook trout Salvelinus fontinalis		128		565		18		Biomass			1435			1295			5914		5914			Salvelinus		Fish		Author reported		wellRO mix
	Fathead minnow Pimephales promelas		220		770		16		Biomass			6194			3569						Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		well water
	Fathead minnow Pimephales promelas		96		620		025		Fry survival			4286			1734			2488		2488			Pimephales		Fish		Author reported half the detection limit
	Zebrafish Danio rerio		83		61		025		Biomass			2344			1102			1102		1102			Danio		Fish		Author reported half the detection limit
	Wood frog Rana sylvatica		115		469		16		MATC-survival		gt		2000		gt		9746		gt		9746		9746		gt		Rana		Amphib - Other Data		Value is from 2007 FW Cu AWQC Appendix C recommendation		aged tap water

	Appendix A Acceptable Acute Toxicity Data of Aluminum to Freshwater Aquatic Animals
	Species		Methoda		Hardness (mgL as CaCO3)		pH		DOC(mgL)			LC50 or EC50 Conc (microgL)		Reference			Normalized Conc - LC50(microgL)			Species Mean Acute Value(microgL)		Genus Mean Acute Value(microgL)			Genus			DOC Note		Dilution water
	Snail Physa sp		S M T		474		659		11		gt		23400		Call 1984 Call et al 1984		gt		48425			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		755		11			30600		Call 1984 Call et al 1984			26986			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		817		11		gt		24700		Call 1984 Call et al 1984		gt		24040			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		746		11			55500		Call 1984 Call et al 1984			50338			35462		35462			Physa		Mollusk		Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Fatmucket Lampsilis siliquoidea		F M T		1060		612		048		gt		6302		Wang et al 2016 Wang et al 2017		gt		29834		gt		29834		29834		gt		Lampsilis		Mollusk		Author reported
	Cladoceran Ceriodaphnia dubia		S M A		500		742		11			1900		McCauley et al 1986			1728			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		S M A		505		786		11			1500		McCauley et al 1986			1294			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		UW-S Lake Superior lab water
	Cladoceran Ceriodaphnia dubia		S M A		500		813		11			2560		McCauley et al 1986			2431			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		R M T		26		75		05			720		ENSR 1992d			1113			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		R M T		46		76		05			1880		ENSR 1992d			2495			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		R M T		96		78		05			2450		ENSR 1992d			2894			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		S U NR		90		715		05			3727		Fort and Stover 1995			5002			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U NR		90		715		05			5673		Fort and Stover 1995			7613			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U NR		89		76		05			2880		Soucek et al 2001			3362			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		601		05			7112		European Al Association 2009			1691			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		605		2			6865		European Al Association 2009			7088			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		609		4			15581		European Al Association 2009			10072			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		601		05			681		European Al Association 2009			1620			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		603		05			1630		European Al Association 2009			3674			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		597		05			1785		European Al Association 2009			4732			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		592		05			1410		European Al Association 2009			4295			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		699		05		gt		1300		European Al Association 2009		gt		4189			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		785		05		gt		5000		European Al Association 2009		gt		7960			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		68		2		gt		10000		European Al Association 2009		gt		20755			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		782		2		gt		15000		European Al Association 2009		gt		11626			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		677		4		gt		10000		European Al Association 2009		gt		15161			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		766		4		gt		15000		European Al Association 2009		gt		8608			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		79		05		gt		2000		European Al Association 2009		gt		3150			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		789		05		gt		2000		European Al Association 2009		gt		3156			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		604		05			1108		European Al Association 2009			850			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		598		2			11371		European Al Association 2009			4817			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		573		4			80467		European Al Association 2009			43570			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		671		05		gt		10000		European Al Association 2009		gt		23786			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		783		05		gt		5000		European Al Association 2009		gt		6344			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		679		2		gt		10000		European Al Association 2009		gt		10394			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		767		2		gt		15000		European Al Association 2009		gt		9082			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		668		4		gt		15000		European Al Association 2009		gt		12461			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		762		4		gt		15000		European Al Association 2009		gt		6332			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		606		2			33868		European Al Association 2009			7920			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		56		4			104842		European Al Association 2009			48800			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		693		05		gt		5000		European Al Association 2009		gt		7126			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		120		788		05		gt		5000		European Al Association 2009		gt		5898			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		120		676		2		gt		15000		European Al Association 2009		gt		12117			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		771		2		gt		15000		European Al Association 2009		gt		8096			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		66		4		gt		15000		European Al Association 2009		gt		10139			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		76		4		gt		15000		European Al Association 2009		gt		5545			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			11971		European Al Association 2010			2699			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			27478		European Al Association 2010			6194			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			11998		European Al Association 2010			2705			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		607		05			92495		European Al Association 2010			1876			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		609		05			31337		European Al Association 2010			6035			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		61		05			33235		European Al Association 2010			6238			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		708		05		gt		8864		European Al Association 2010		gt		2534			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		779		05		gt		42783		European Al Association 2010		gt		6930			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		753		05			13204		European Al Association 2010			244			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		600		601		05			46326		European Al Association 2010			3798			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		600		599		05		gt		8590		European Al Association 2010		gt		7366			5094				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia reticulata		F M T		451		60		11			304		Shephard 1983			2009			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia reticulata		F M T		40		55		11			362		Shephard 1983			55307			10542		7328			Ceriodaphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake SuperiorDI Mix
	Cladoceran Daphnia magna		S U NR		453		774		11			3900		Biesinger and Christensen 1972			3382			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Daphnia magna		S M T		220		76		16			38200		Kimball 1978			20303			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Well water
	Cladoceran Daphnia magna		S U T		451		725		11			2800		Shephard 1983			2868			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Daphnia magna		S U T		168		599		05			5000		European Al Association 2009		gt		2265			-				Daphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Daphnia magna		S U T		168		792		05			7950		European Al Association 2009			920			3332		3332			Daphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Ostracod Stenocypris major		R M T		1563		651		16			310196		Shuhaimi-Othman et al 2011a			10216			10216		10216			Stenocypris		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (Malaysia)
	Amphipod Crangonyx pseudogracilis		R U T		50		675		16			9190		Martin and Holdich 1986			12174			12174		12174			Crangonyx		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (UK)
	Amphipod Hyalella azteca		F M T		105		613		048		gt		5997		Wang et al 2016 Wang et al 2017		gt		28002		gt		28002		28002		gt		Hyalella		Invert		Author reported
	Stonefly Acroneuria sp		S M T		474		746		11		gt		22600		Call et al 1984		gt		20498		gt		20498		20498		gt		Acroneuria		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Midge Chironomus plumosus		S U T		80		70		16			30000		Fargasova 2001			25361			25361		25361			Chironomus		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (Slovakia)
	Midge Paratanytarsus dissimilis		S M T		1743		728		28		gt		77700		Lamb and Bailey 1981 1983		gt		62318		gt		62318		62318		gt		Paratanytarsus		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Liberty Lake Washington
	Rainbow trout Oncorhynchus mykiss		F M T		274		758		05		gt		9840		Gundersen et al 1994		gt		5745			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		452		762		05		gt		8070		Gundersen et al 1994		gt		5212			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		895		758		05		gt		8160		Gundersen et al 1994		gt		6807			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		1304		758		05		gt		8200		Gundersen et al 1994		gt		7662			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		232		825		05			6170		Gundersen et al 1994			1190			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		350		826		05			6170		Gundersen et al 1994			1523			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		836		829		05			7670		Gundersen et al 1994			3205			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		1158		830		05			6930		Gundersen et al 1994			3554			3661		3661			Oncorhynchus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Atlantic salmon Salmo salar		S U T		68		55		05			584		Hamilton and Haines 1995			21042			-				Salmo			Value is from 2007 FW Cu AWQC Appendix C recommendation		Soft recon
	Atlantic salmon Salmo salar		S U T		68		65		05			599		Hamilton and Haines 1995			2430			7151		7151			Salmo		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Soft recon
	Brook trout Salvelinus fontinalis		S U T		40		56		16			6530		Tandjung 1982			31767			-				Salvelinus			Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Brook trout Salvelinus fontinalis		S U T		18		56		16			3400		Tandjung 1982			28391			-				Salvelinus			Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Brook trout Salvelinus fontinalis		S U T		2		56		16			370		Tandjung 1982			13663			23097		23097			Salvelinus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Green sunfish Lepomis cyanellus		S M T		474		755		11		gt		50000		Call et al 1984		gt		24028		gt		24028		24028		gt		Lepomis		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Rio Grande silvery minnow Hybognathus amarus		R M T		140		81		05		gt		59100		Buhl 2002		gt		39414		gt		39414		39414		gt		Hybognathus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon water
	Fathead minnow Pimephales promelas		S M T		474		761		11		gt		48200		Call et al 1984		gt		21522			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Fathead minnow Pimephales promelas		S M T		474		805		11		gt		49800		Call et al 1984		gt		12972			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Fathead minnow Pimephales promelas		R M T		26		78		05			1160		ENSR 1992c			477			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		46		76		05			8180		ENSR 1992c			5446			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		96		81		05			20300		ENSR 1992c			10966			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		194		81		05			44800		ENSR 1992c			35847			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		140		81		05		gt		59100		Buhl 2002		gt		39414			10168		10168			Pimephales		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon water
	Smallmouth bass Micropterus dolomieui		S M T		1245		505		16			130		Kane and Rabeni 1987			3929			3929		3929			Micropterus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Green tree frog Hyla cinerea		R M T		455		549		05		gt		4052		Jung and Jagoe 1995		gt		20016		gt		20016		20016		gt		Hyla		Amphibian		Value is from 2007 FW Cu AWQC Appendix C recommendation		artificial soft recon

	Normalization Chemistry
	pH		7					FAV		2741
	Hardness		100					CMC		1400
	DOC		1					CCC		390
	Acute Ranked GMAV											Chronic Ranked GMCV
	Rank			GMAV		Genus								Rank			GMCV		Genus
	18		gt		62318		Paratanytarsus		Invert							12			17098		Aeolosoma		Invert
	17		gt		39414		Hybognathus		Fish							11		gt		9746		Rana		Amphib - Other Data
	16			35462		Physa		Mollusk							10			5908		Chironomus		Invert
	15		gt		29834		Lampsilis		Mollusk							9			4877		Lymnaea		Mollusk
	14		gt		28002		Hyalella		Invert							8			2555		Brachionus		Invert
	13			25361		Chironomus		Invert							7			2488		Pimephales		Fish
	12		gt		24028		Lepomis		Fish							6			1713		Hyalella		Invert
	11			23097		Salvelinus		Fish							5			1182		Ceriodaphnia		Invert
	10		gt		20498		Acroneuria		Invert							4			1102		Danio		Fish
	9		gt		20016		Hyla		Amphibian							3			1042		Lampsilis		Mollusk
	8			12174		Crangonyx		Invert							2			591		Salvelinus		Fish
	7			10216		Stenocypris		Invert							1			509		Salmo		Fish
	6			10168		Pimephales		Fish
	5			7328		Ceriodaphnia		Invert
	4			7151		Salmo		Fish
	3			3929		Micropterus		Fish
	2			3661		Oncorhynchus		Fish
	1			3332		Daphnia		Invert
		N		Rank		Genus		GMAV		ln(GMAV)		ln(GMAV)2		P=R(N+1)		sqrt(P)				N		Rank		Genus		GMCV		ln(GMCV)		ln(GMCV)2		P=R(N+1)		sqrt(P)
		18		4		Salmo		7151		887		7876		0211		0459				12		4		Danio		1102		700		4907		0308		0555
			3		Micropterus		3929		828		6849		0158		0397					3		Lampsilis		1042		695		4829		0231		0480
			2		Oncorhynchus		3661		821		6733		0105		0324					2		Salvelinus		591		638		4074		0154		0392
			1		Daphnia		3332		811		6579		0053		0229					1		Salmo		509		623		3883		0077		0277
			Sum				3347		2804		0526		141					Sum				2657		1769		0769		170
			S2 =		1223										S2 =		1080
			L =		7134										L =		5242
			A =		7916										A =		5976
			FAV =		2741										FCV =		3940
			CMC =		1371

				All concentrations reported are microgL total Aluminum
				lt-----Click Button after entering data
	ENTER DATA HERE									ERRORNUM		ACUTE														CHRONIC
										Rank 1				Rank 2				Rank 3				Rank 4					Rank 1				Rank 2				Rank 3				Rank 4
	SiteName		DOC(mgL)		Hardness(mgL as CaCO3)		pH			FAV		CMC		CCC			Genus		GMAV			Genus		GMAV			Genus		GMAV			Genus		GMAV				Genus		GMCV			Genus		GMCV			Genus		GMCV			Genus		GMCV
	Site 1		50		25		9		75428737034162		35229324666008		1800		770			Daphnia			33452		Ceriodaphnia			73576		Stenocypris			102576		Crangonyx			122230			Lampsilis			10463		Ceriodaphnia			11865		Hyalella			17200		Brachionus			25648
	Site 2		50		50		9		75428737034162		31204991509063		1600		680			Daphnia			29631		Ceriodaphnia			65171		Stenocypris			90859		Crangonyx			108267			Lampsilis			9268		Ceriodaphnia			10510		Hyalella			15236		Brachionus			22718
	Site 3		50		75		9		75428737034162		29067527928199		1500		640			Daphnia			27601		Ceriodaphnia			60707		Stenocypris			84635		Crangonyx			100851			Lampsilis			8633		Ceriodaphnia			9790		Hyalella			14192		Brachionus			21162
	Site 4		50		100		9		75428737034162		27640367912592		1400		600			Daphnia			26246		Ceriodaphnia			57726		Stenocypris			80480		Crangonyx			95899			Lampsilis			8209		Ceriodaphnia			9309		Hyalella			13495		Brachionus			20123
	Site 5		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 6		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 7		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 8		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 9		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 10		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 11		50		25		85		75428737034162		63245275664384		3200		1400			Daphnia			60055		Ceriodaphnia			132086		Stenocypris			184149		Crangonyx			219432			Lampsilis			18783		Ceriodaphnia			21301		Hyalella			30879		Brachionus			46045
	Site 12		50		50		85		75428737034162		61388044609926		3100		1300			Daphnia			58291		Ceriodaphnia			128208		Stenocypris			178742		Crangonyx			212988			Lampsilis			18232		Ceriodaphnia			20676		Hyalella			29972		Brachionus			44693
	Site 13		50		75		85		75428737034162		60327020429132		3000		1300			Daphnia			57284		Ceriodaphnia			125992		Stenocypris			175652		Crangonyx			209307			Lampsilis			17917		Ceriodaphnia			20318		Hyalella			29454		Brachionus			43920
	Site 14		50		100		85		75428737034162		59585352130146		3000		1300			Daphnia			56580		Ceriodaphnia			124443		Stenocypris			173493		Crangonyx			206734			Lampsilis			17696		Ceriodaphnia			20068		Hyalella			29092		Salmo			41264
	Site 15		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 16		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 17		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 18		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 19		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 20		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370

I Screen toxicological studies for high quality data

bull First step in all criteria developmentndash Data are evaluated based on the data quality objectives

outlined in the 1985 Guidelines methodology and other toxicity testing recommendations (ie EPA ASTM)

ndash High quality data are used quantitatively to develop criteria magnitudes

ndash Lower quality data are used qualitatively if appropriatendash Lowest quality data are considered unacceptable and not

usedbull Data are grouped into acute and chronic exposures

10

Aluminum Toxicity Databull The updated freshwater aluminum criteria meets the

acute and chronic minimum data requirements outlined in EPArsquos 1985 Guidelines

InvertebrateSpecies

Fish Species

AmphibianSpecies

Total Genera

Acute1988 AWQC 8 7 - 142017 Draft AWQC 11 8 1 18

Chronic1988 AWQC 2 1 - 32017 Draft AWQC 7 4 - 11

11 1988 AWQC Chronic CCC based on two studies for the striped

bass and brook trout

Aluminum Toxicity Data Freshwater mussels have been

suggested to be potentially sensitive to aluminum

EPA worked collaboratively with USGSrsquos Columbia Environmental Research Center (CERC) to have acute and chronic aluminum toxicity tests conducted with a unionid mussel (fatmucket Lampsilis siliquoidea) in water-only exposures to obtain additional data on invertebrates New data on amphipod (Hyalella azteca)

were collected to confirm laboratory conditions

12

Aluminum Toxicity Data

13

bull New data and models examinedndash Data submitted to EU REACH program

bull New freshwater aluminum toxicity data generated by Oregon State University

bull 2016 BLM model provided by the European Aluminum Association

ndash Saltwater data provided by Australia bull Insufficient data to develop estuarinemarine acute or chronic

criteria at this timendash Multiple Linear Regression (MLR) model developed by

DeForest et al 2017bull DeForest DK KV Brix LM Tear and WJ Adams 2017 Multiple Linear Regression (MLR) models for predicting chronic aluminum

toxicity to freshwater aquatic organisms and developing water quality guidelines Environ Toxicol Chem (DOI101002etc3922)

14

Minimum Dataset For Freshwater Criteria Derivation

SALMONID SECONDFISHFAMILY

CHORDATA

PLANKTONICCRUSTACEAN

BENTHIC CRUSTACEAN

INSECT ROTIFERA ANNELIDA MOLLUSCA

OTHERINSECT ORMOLLUSCA

EstuarineMarine Minimum Data Requirements

15

Family Minimum Data Requirement EstuarineMarine

Acute(Phylum Family Genus)

Chronic(Phylum Family Genus)

Family in the phylum Chordata - -

Family in the phylum Chordata - -

Either the Mysidae or Penaeidae family - -

Family in a phylum other than Arthropoda or Chordata Mollusca Ostreidae Crassostrea -

Family in a phylum other than Chordata Annelida Nereididae Neanthes -

Family in a phylum other than Chordata Annelida Capitellidae Capitella -

Family in a phylum other than Chordata Annelida Ctenodrilidae Ctenodrilus -

Any other family Arthropoda Ameiridae Nitokra -

II Evaluate water quality conditions to determine applicability

bull Criteria are often based on one or more water quality conditions (eg hardness) that affect toxicity of the material to test organisms

bull Aluminum toxicity is highly dependent on the pH of the test waterndash The pH of test waters affects the amount and form

of aluminum that is bioavailable to test speciesndash Hardness and DOC (Dissolved Organic Carbon)

also influence the amount of aluminum that is bioavailable

16

Multiple Bioavailability Modeling Approaches Underwent External Peer Review

17

bull Biotic Ligand Modelsbull Full aluminum BLM (2016 model version) ndash Temperature pH

Dissolved organic carbon (DOC) Major cations (calcium [Ca] magnesium [Mg] sodium [Na] and potassium [K]) Major anions (sulfate [SO4] and chloride [Cl]) Alkalinity and Sulfide

bull Simplified aluminum BLM - pH hardness DOC temperature

bull Multilinear Regression Equationsbull pH and Hardness

Accounting for Effects of Water Chemistry on Bioavailability and Toxicity

bull Water quality criteria can be modified quantitatively when enough data are available to demonstrate that those conditions similarly affect the results with a variety of speciesndash High quality data in the draft aluminum document demonstrated

that pH DOC and hardness affected a variety of species in a similar way when exposed to aluminum concentrations

bull Recent publications by Cardwell et al (2017) and Gensemer et al (2017) summarized short-term aluminum chronic toxicity data across a range of DOC pH and hardness values for three species

18

Multiple Linear Regression (MLR) Models to Account for Effects of Water Chemistry

bull MLR models were developed for each species (DeForest et al 2017)ndash 119862119862 119889119889119906119906119887119887119894119894119886119886 11986411986411986211986220=119890119890[minus41026+[0525timesln(119863119863119874119874119862119862)]+[2201timesln(ℎ119886119886119903119903119889119889)]+(11282times119901119901119867119867)minus(0633times1199011199011198671198672)minus[0264times119901119901119867119867ln(ℎ119886119886119903119903119889119889)]]

ndash 119875119875 119901119901119903119903119900119900119898119898119890119890119897119897119886119886119904119904 11986411986411986211986220=119890119890[minus14029+[0503timesln(119863119863119874119874119862119862)]+[3443timesln(ℎ119886119886119903119903119889119889)]+(3131times119901119901119867119867)minus[0494times119901119901119867119867ln(ℎ119886119886119903119903119889119889)]]

bull These models were applied to both the acute and chronic aluminum toxicity datandash The C dubia model was applied to invertebrate datandash The P promelas model was applied to vertebrate data

19

III Normalize toxicity data in species sensitivity distribution to a set of water

quality conditionsbull Just like single parameter hardness models the MLR

models were used to normalize the aluminum toxicity data to a single set of water quality conditionsndash As an illustration for one set of calculations the draft

aluminum AWQC set these conditions to pH 7 hardness of 100 mgL and DOC of 1 mgL

bull This allows for comparisons to be made between species based on their sensitivity to aluminumndash Apples to apples

20

IV Construct sensitivity distribution to determine criteria at set conditions

21

Figure 8 Ranked Summary of Total Aluminum Genus Mean Acute Values (GMAVs) - Freshwater at pH 7 Hardness of 100 mgL and DOC of 1 mgL

IV Construct sensitivity distribution to determine criteria at set conditions

22

Figure 10 Ranked Summary of Total Aluminum Genus Mean Chronic Values (GMCVs) ndash Freshwater Supplemented with Other Data to Fulfill Missing MDRs at pH 7 Hardness of 100 mgL and DOC of 1 mgL

Aluminum Criteria Calculatorbull The relative ranking of genera in the sensitivity

distribution to which the toxicity data are normalized will change based on the set of water chemistry conditions (ie pH hardness and DOC)

bull To make calculating aluminum criteria easier EPA developed an Aluminum Criteria Calculatorndash Automated so that user only needs to enter water chemistry

conditions of interestndash Allows quick calculations for a variety of conditionsndash Limits calculations to the recommended conditions for which

the MLR models were developed were specified for hardness and DOC

bull No extrapolation beyond hardness and DOC empirical data bounds

23

Aluminum Criteria Calculator SD Example

24

pH=7 Hardness=100 DOC=1 pH=6 Hardness=25 DOC=1

Chronic Ranked GMCVRank GMCV Genus

12 17098 Aeolosoma Invert11 gt 9746 Rana Amphib - Other Data10 5908 Chironomus Invert

9 4877 Lymnaea Mollusk8 2555 Brachionus Invert7 2488 Pimephales Fish6 1713 Hyalella Invert5 1182 Ceriodaphnia Invert4 1102 Danio Fish3 1042 Lampsilis Mollusk2 591 Salvelinus Fish1 509 Salmo Fish

FCV = 3940 microgL total aluminum

Chronic Ranked GMCVRank GMCV Genus

12 gt 2131 Rana Amphib - Other Data11 1710 Aeolosoma Invert10 591 Chironomus Invert

9 544 Pimephales Fish8 488 Lymnaea Mollusk7 255 Brachionus Invert6 241 Danio Fish5 171 Hyalella Invert4 129 Salvelinus Fish3 118 Ceriodaphnia Invert2 111 Salmo Fish1 104 Lampsilis Mollusk

FCV = 987 microgL total aluminum

Multiple Scenarios

Run Scenarios

Summary Sheet

Acute Dataset

Chronic Dataset

Multiple Scenarios

Run Scenarios

Summary Sheet

Acute Dataset

Chronic Dataset

Boundaries of the Criteria Outputsbull MLR models were developed using data that

encompassndash DOC range of 008-5 mgLndash Hardness range of 98-127 mgL (as CaCO3)ndash pH range of 6-81

bull MLR criteria outputs are bounded atndash Maximum of 150 mgL total hardness (as CaCO3)

ndash DOC of 50 mgLndash pH range of the model is 50 to 90

25

Aluminum Criteria Look-Up Tables

26

bull EPA also provided criteria look-up tables in Appendix K

Har

dnes

s

CCC(microgL total aluminum)

(DOC=05 mgL)

pH 50 pH 55 pH 60 pH 65 pH 70 pH 75 pH 80 pH 85 pH 90

25 23 a 14 b 69 c 160 f 350 f 620 i 540 b 410 a 230 a

50 43 a 24 b 100 d 170 f 310 f 630 f 670 j 400 a 200 a

75 61 a 33 b 130 e 180 f 290 f 530 f 730 i 390 a 190 a

100 78 a 41 b 150 e 190 f 280 g 460 f 770 c 390 a 180 a

150 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

200 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

250 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

300 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

350 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

400 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

Aluminum Criteria Look-Up Tables

27

bull EPA also provided criteria look-up tables in Appendix K

Har

dnes

s

CCC(microgL total aluminum)

(DOC=10 mgL)

pH 50 pH 55 pH 60 pH 65 pH 70 pH 75 pH 80 pH 85 pH 90

25 33 a 21 b 99 c 220 f 490 f 900 i 780 b 590 a 330 a

50 61 a 35 b 150 e 250 f 440 f 890 f 960 j 580 a 290 a

75 88 a 47 b 180 e 260 f 410 f 740 f 1100 i 570 a 270 a

100 11 a 59 b 210 e 270 f 390 g 650 f 1100 c 560 b 260 a

150 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

200 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

250 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

300 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

350 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

400 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

Aluminum Criteria Look-Up Tables

28

bull EPA also provided criteria look-up tables in Appendix K

Har

dnes

s

CCC(microgL total aluminum)

(DOC=25 mgL)

pH 50 pH 55 pH 60 pH 65 pH 70 pH 75 pH 80 pH 85 pH 90

25 54 a 34 b 160 c 350 f 770 f 1500 i 1300 b 960 a 540 a

50 99 a 57 b 230 e 390 f 690 f 1400 f 1600 j 930 a 470 a

75 14 a 77 b 290 e 410 f 640 f 1200 f 1700 i 920 a 440 a

100 18 a 96 b 330 e 420 f 620 g 1000 f 1800 d 920 b 420 a

150 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

200 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

250 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

300 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

350 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

400 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

Aluminum Criteria Look-Up Tables

29

bull EPA also provided criteria look-up tables in Appendix K

Har

dnes

s

CCC(microgL total aluminum)

(DOC=50 mgL)

pH 50 pH 55 pH 60 pH 65 pH 70 pH 75 pH 80 pH 85 pH 90

25 77 a 49 b 230 c 500 f 1100 f 2100 c 1900 b 1400 a 770 a

50 14 a 82 b 330 e 540 f 970 f 2000 f 2200 j 1300 a 680 a

75 20 a 110 b 410 e 570 f 900 f 1600 f 2500 i 1300 a 640 a

100 26 a 140 b 460 f 590 f 880 g 1400 f 2500 d 1300 b 600 a

150 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

200 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

250 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

300 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

350 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

400 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

Status

30

bull Draft Document Released to the Federal Register on 72817ndash Public comment period of 60 days ndash Due Date for Comments is 92617

bull Revisions will be made considering public commentsbull Subsequent EPA intra-agency review of final criteria

document will then be conducted bull Publication of final criteria document planned for Late Fall

2017

Federal Register Webpagehttpswwwfederalregistergovdocuments201

707282017-15968request-for-scientific-views-draft-updated-aquatic-life-ambient-water-quality-criteria-for-aluminum

Aluminum Criteria Webpagehttpswwwepagovwqc2017-draft-aquatic-life-

criteria-aluminum-freshwater-documents

31

	Appendix C Acceptable Chronic Toxicity Data of Aluminum to Freshwater Aquatic Animals
									MLR equation
	Species		Hardness (mgL as CaCO3)		pH		DOC(mgL)		EC20 Endpoint			EC20(microgL)			Normalized Conc- EC20(microgL)			SMCV-EC20(microgL)		GMCV-EC20(microgL)			Genus			DOC Notes		Dilution water
	Oligochaete Aeolosoma sp		48		6		025		Reproduction (population size)			1235			1710			1710		1710			Aeolosoma		Invert		Author reported half the detection limit
	Rotifer Brachionus calyciflorus		100		62		025		Reproduction (population size)			431			255			255		255			Brachionus		Invert		Author reported half the detection limit
	Great pond snail Lymnaea stagnalis		117		61		025		Biomass			746			488			488		488			Lymnaea		Mollusk		Author reported half the detection limit
	Fatmucket Lampsilis siliquoidea		1055		604		040		Biomass			169			104			104		104			Lampsilis		Mollusk		Author reported
	Cladoceran Ceriodaphnia dubia		50		715		11		Reproduction - youngstarting adult			1780			190						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		26		73		05		Reproduction - youngfemale			1557			272						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		46		75		05		Reproduction - youngfemale			8087			110						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		96		79		05		Reproduction - youngfemale			6519			80						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		194		81		05		Reproduction - youngfemale			6836			89						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		255		703		05		Reproduction (youngfemale)			250			56						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		122		713		05		Reproduction (youngfemale)			860			106						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		25		798		05		Reproduction (youngfemale)			700			101						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		61		803		05		Reproduction (youngfemale)			1010			136						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		122		810		05		Reproduction (youngfemale)			870			116						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		255		633		05		Reproduction (youngfemale)			260			176						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		121		636		05		Reproduction (youngfemale)			390			110			118		118			Ceriodaphnia		Invert		Author reported
	Amphipod Hyalella azteca		95		61		051		Biomass (28 d)			1993			101						Hyalella			Author reported
	Amphipod Hyalella azteca		106		604		033		Biomass (28 d)			425			289			171		171			Hyalella		Invert		Author reported
	Midge Chironomus riparius		118		558		18		Adult midge emergence			2955			119						Chironomus			Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Midge Chironomus riparius		119		505		18		Adult midge emergence			8442			2258						Chironomus			Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Midge Chironomus riparius		91		66		051		Reproduction ( of eggscase)			3387			766			591		591			Chironomus		Invert		Author reported
	Atlantic salmon Salmo salar		127		57		18		Biomass			6156			1112			1112		1112			Salmo		Fish		Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Brook trout Salvelinus fontinalis		123		655		19		Biomass			1644			59						Salvelinus			Author reported		wellRO mix
	Brook trout Salvelinus fontinalis		128		565		18		Biomass			1435			283			1293		1293			Salvelinus		Fish		Author reported		wellRO mix
	Fathead minnow Pimephales promelas		220		770		16		Biomass			6194			781						Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		well water
	Fathead minnow Pimephales promelas		96		620		025		Fry survival			4286			379			544		544			Pimephales		Fish		Author reported half the detection limit
	Zebrafish Danio rerio		83		61		025		Biomass			2344			241			241		241			Danio		Fish		Author reported half the detection limit
	Wood frog Rana sylvatica		115		469		16		MATC-survival		gt		2000		gt		2131		gt		2131		2131		gt		Rana		Amphib - Other Data		Value is from 2007 FW Cu AWQC Appendix C recommendation		aged tap water

	Appendix A Acceptable Acute Toxicity Data of Aluminum to Freshwater Aquatic Animals
	Species		Methoda		Hardness (mgL as CaCO3)		pH		DOC(mgL)			LC50 or EC50 Conc (microgL)		Reference			Normalized Conc - LC50(microgL)			Species Mean Acute Value(microgL)		Genus Mean Acute Value(microgL)			Genus			DOC Note		Dilution water
	Snail Physa sp		S M T		474		659		11		gt		23400		Call 1984 Call et al 1984		gt		4843			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		755		11			30600		Call 1984 Call et al 1984			2699			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		817		11		gt		24700		Call 1984 Call et al 1984		gt		2404			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		746		11			55500		Call 1984 Call et al 1984			5034			3546		3546			Physa		Mollusk		Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Fatmucket Lampsilis siliquoidea		F M T		1060		612		048		gt		6302		Wang et al 2016 Wang et al 2017		gt		2983		gt		2983		2983		gt		Lampsilis		Mollusk		Author reported
	Cladoceran Ceriodaphnia dubia		S M A		500		742		11			1900		McCauley et al 1986			173			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		S M A		505		786		11			1500		McCauley et al 1986			129			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		UW-S Lake Superior lab water
	Cladoceran Ceriodaphnia dubia		S M A		500		813		11			2560		McCauley et al 1986			243			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		R M T		26		75		05			720		ENSR 1992d			111			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		R M T		46		76		05			1880		ENSR 1992d			250			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		R M T		96		78		05			2450		ENSR 1992d			289			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		S U NR		90		715		05			3727		Fort and Stover 1995			500			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U NR		90		715		05			5673		Fort and Stover 1995			761			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U NR		89		76		05			2880		Soucek et al 2001			336			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		601		05			7112		European Al Association 2009			169			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		605		2			6865		European Al Association 2009			709			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		609		4			15581		European Al Association 2009			1007			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		601		05			681		European Al Association 2009			162			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		603		05			1630		European Al Association 2009			367			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		597		05			1785		European Al Association 2009			473			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		592		05			1410		European Al Association 2009			429			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		699		05		gt		1300		European Al Association 2009		gt		419			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		785		05		gt		5000		European Al Association 2009		gt		796			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		68		2		gt		10000		European Al Association 2009		gt		2076			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		782		2		gt		15000		European Al Association 2009		gt		1163			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		677		4		gt		10000		European Al Association 2009		gt		1516			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		766		4		gt		15000		European Al Association 2009		gt		861			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		79		05		gt		2000		European Al Association 2009		gt		315			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		789		05		gt		2000		European Al Association 2009		gt		316			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		604		05			1108		European Al Association 2009			85			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		598		2			11371		European Al Association 2009			482			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		573		4			80467		European Al Association 2009			4357			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		671		05		gt		10000		European Al Association 2009		gt		2379			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		783		05		gt		5000		European Al Association 2009		gt		634			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		679		2		gt		10000		European Al Association 2009		gt		1039			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		767		2		gt		15000		European Al Association 2009		gt		908			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		668		4		gt		15000		European Al Association 2009		gt		1246			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		762		4		gt		15000		European Al Association 2009		gt		633			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		606		2			33868		European Al Association 2009			792			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		56		4			104842		European Al Association 2009			4880			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		693		05		gt		5000		European Al Association 2009		gt		713			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		120		788		05		gt		5000		European Al Association 2009		gt		590			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		120		676		2		gt		15000		European Al Association 2009		gt		1212			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		771		2		gt		15000		European Al Association 2009		gt		810			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		66		4		gt		15000		European Al Association 2009		gt		1014			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		76		4		gt		15000		European Al Association 2009		gt		555			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			11971		European Al Association 2010			270			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			27478		European Al Association 2010			619			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			11998		European Al Association 2010			270			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		607		05			92495		European Al Association 2010			188			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		609		05			31337		European Al Association 2010			604			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		61		05			33235		European Al Association 2010			624			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		708		05		gt		8864		European Al Association 2010		gt		253			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		779		05		gt		42783		European Al Association 2010		gt		693			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		753		05			13204		European Al Association 2010			24			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		600		601		05			46326		European Al Association 2010			380			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		600		599		05		gt		8590		European Al Association 2010		gt		737			509				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia reticulata		F M T		451		60		11			304		Shephard 1983			201			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia reticulata		F M T		40		55		11			362		Shephard 1983			5531			1054		733			Ceriodaphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake SuperiorDI Mix
	Cladoceran Daphnia magna		S U NR		453		774		11			3900		Biesinger and Christensen 1972			338			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Daphnia magna		S M T		220		76		16			38200		Kimball 1978			2030			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Well water
	Cladoceran Daphnia magna		S U T		451		725		11			2800		Shephard 1983			287			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Daphnia magna		S U T		168		599		05			5000		European Al Association 2009		gt		227			-				Daphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Daphnia magna		S U T		168		792		05			7950		European Al Association 2009			92			333		333			Daphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Ostracod Stenocypris major		R M T		1563		651		16			310196		Shuhaimi-Othman et al 2011a			1022			1022		1022			Stenocypris		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (Malaysia)
	Amphipod Crangonyx pseudogracilis		R U T		50		675		16			9190		Martin and Holdich 1986			1217			1217		1217			Crangonyx		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (UK)
	Amphipod Hyalella azteca		F M T		105		613		048		gt		5997		Wang et al 2016 Wang et al 2017		gt		2800		gt		2800		2800		gt		Hyalella		Invert		Author reported
	Stonefly Acroneuria sp		S M T		474		746		11		gt		22600		Call et al 1984		gt		2050		gt		2050		2050		gt		Acroneuria		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Midge Chironomus plumosus		S U T		80		70		16			30000		Fargasova 2001			2536			2536		2536			Chironomus		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (Slovakia)
	Midge Paratanytarsus dissimilis		S M T		1743		728		28		gt		77700		Lamb and Bailey 1981 1983		gt		6232		gt		6232		6232		gt		Paratanytarsus		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Liberty Lake Washington
	Rainbow trout Oncorhynchus mykiss		F M T		274		758		05		gt		9840		Gundersen et al 1994		gt		1256			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		452		762		05		gt		8070		Gundersen et al 1994		gt		1140			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		895		758		05		gt		8160		Gundersen et al 1994		gt		1489			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		1304		758		05		gt		8200		Gundersen et al 1994		gt		1676			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		232		825		05			6170		Gundersen et al 1994			260			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		350		826		05			6170		Gundersen et al 1994			333			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		836		829		05			7670		Gundersen et al 1994			701			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		1158		830		05			6930		Gundersen et al 1994			777			801		801			Oncorhynchus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Atlantic salmon Salmo salar		S U T		68		55		05			584		Hamilton and Haines 1995			4602			-				Salmo			Value is from 2007 FW Cu AWQC Appendix C recommendation		Soft recon
	Atlantic salmon Salmo salar		S U T		68		65		05			599		Hamilton and Haines 1995			531			1564		1564			Salmo		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Soft recon
	Brook trout Salvelinus fontinalis		S U T		40		56		16			6530		Tandjung 1982			6947			-				Salvelinus			Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Brook trout Salvelinus fontinalis		S U T		18		56		16			3400		Tandjung 1982			6209			-				Salvelinus			Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Brook trout Salvelinus fontinalis		S U T		2		56		16			370		Tandjung 1982			2988			5051		5051			Salvelinus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Green sunfish Lepomis cyanellus		S M T		474		755		11		gt		50000		Call et al 1984		gt		5255		gt		5255		5255		gt		Lepomis		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Rio Grande silvery minnow Hybognathus amarus		R M T		140		81		05		gt		59100		Buhl 2002		gt		8620		gt		8620		8620		gt		Hybognathus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon water
	Fathead minnow Pimephales promelas		S M T		474		761		11		gt		48200		Call et al 1984		gt		4707			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Fathead minnow Pimephales promelas		S M T		474		805		11		gt		49800		Call et al 1984		gt		2837			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Fathead minnow Pimephales promelas		R M T		26		78		05			1160		ENSR 1992c			104			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		46		76		05			8180		ENSR 1992c			1191			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		96		81		05			20300		ENSR 1992c			2398			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		194		81		05			44800		ENSR 1992c			7839			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		140		81		05		gt		59100		Buhl 2002		gt		8620			2224		2224			Pimephales		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon water
	Smallmouth bass Micropterus dolomieui		S M T		1245		505		16			130		Kane and Rabeni 1987			859			859		859			Micropterus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Green tree frog Hyla cinerea		R M T		455		549		05		gt		4052		Jung and Jagoe 1995		gt		4377		gt		4377		4377		gt		Hyla		Amphibian		Value is from 2007 FW Cu AWQC Appendix C recommendation		artificial soft recon

	Normalization Chemistry
	pH		6					FAV		360
	Hardness		25					CMC		180
	DOC		1					CCC		99
	Acute Ranked GMAV											Chronic Ranked GMCV
	Rank			GMAV		Genus								Rank			GMCV		Genus
	18		gt		8620		Hybognathus		Fish							12		gt		2131		Rana		Amphib - Other Data
	17		gt		6232		Paratanytarsus		Invert							11			1710		Aeolosoma		Invert
	16		gt		5255		Lepomis		Fish							10			591		Chironomus		Invert
	15			5051		Salvelinus		Fish							9			544		Pimephales		Fish
	14		gt		4377		Hyla		Amphibian							8			488		Lymnaea		Mollusk
	13			3546		Physa		Mollusk							7			255		Brachionus		Invert
	12		gt		2983		Lampsilis		Mollusk							6			241		Danio		Fish
	11		gt		2800		Hyalella		Invert							5			171		Hyalella		Invert
	10			2536		Chironomus		Invert							4			129		Salvelinus		Fish
	9			2224		Pimephales		Fish							3			118		Ceriodaphnia		Invert
	8		gt		2050		Acroneuria		Invert							2			111		Salmo		Fish
	7			1564		Salmo		Fish							1			104		Lampsilis		Mollusk
	6			1217		Crangonyx		Invert
	5			1022		Stenocypris		Invert
	4			859		Micropterus		Fish
	3			801		Oncorhynchus		Fish
	2			733		Ceriodaphnia		Invert
	1			333		Daphnia		Invert
		N		Rank		Genus		GMAV		ln(GMAV)		ln(GMAV)2		P=R(N+1)		sqrt(P)				N		Rank		Genus		GMCV		ln(GMCV)		ln(GMCV)2		P=R(N+1)		sqrt(P)
		18		4		Micropterus		859		676		4564		0211		0459				12		4		Salvelinus		129		486		2364		0308		0555
			3		Oncorhynchus		801		669		4469		0158		0397					3		Ceriodaphnia		118		477		2277		0231		0480
			2		Ceriodaphnia		733		660		4352		0105		0324					2		Salmo		111		471		2220		0154		0392
			1		Daphnia		333		581		3374		0053		0229					1		Lampsilis		104		465		2159		0077		0277
			Sum				2585		1676		0526		141					Sum				1899		902		0769		170
			S2 =		1987										S2 =		059
			L =		4890										L =		4420
			A =		5887										A =		4592
			FAV =		360										FCV =		987
			CMC =		180

				All concentrations reported are microgL total Aluminum
				lt-----Click Button after entering data
	ENTER DATA HERE									ERRORNUM		ACUTE														CHRONIC
										Rank 1				Rank 2				Rank 3				Rank 4					Rank 1				Rank 2				Rank 3				Rank 4
	SiteName		DOC(mgL)		Hardness(mgL as CaCO3)		pH			FAV		CMC		CCC			Genus		GMAV			Genus		GMAV			Genus		GMAV			Genus		GMAV				Genus		GMCV			Genus		GMCV			Genus		GMCV			Genus		GMCV
	Site 1		50		25		9		75428737034162		35229324666008		1800		770			Daphnia			33452		Ceriodaphnia			73576		Stenocypris			102576		Crangonyx			122230			Lampsilis			10463		Ceriodaphnia			11865		Hyalella			17200		Brachionus			25648
	Site 2		50		50		9		75428737034162		31204991509063		1600		680			Daphnia			29631		Ceriodaphnia			65171		Stenocypris			90859		Crangonyx			108267			Lampsilis			9268		Ceriodaphnia			10510		Hyalella			15236		Brachionus			22718
	Site 3		50		75		9		75428737034162		29067527928199		1500		640			Daphnia			27601		Ceriodaphnia			60707		Stenocypris			84635		Crangonyx			100851			Lampsilis			8633		Ceriodaphnia			9790		Hyalella			14192		Brachionus			21162
	Site 4		50		100		9		75428737034162		27640367912592		1400		600			Daphnia			26246		Ceriodaphnia			57726		Stenocypris			80480		Crangonyx			95899			Lampsilis			8209		Ceriodaphnia			9309		Hyalella			13495		Brachionus			20123
	Site 5		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 6		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 7		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 8		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 9		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 10		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 11		50		25		85		75428737034162		63245275664384		3200		1400			Daphnia			60055		Ceriodaphnia			132086		Stenocypris			184149		Crangonyx			219432			Lampsilis			18783		Ceriodaphnia			21301		Hyalella			30879		Brachionus			46045
	Site 12		50		50		85		75428737034162		61388044609926		3100		1300			Daphnia			58291		Ceriodaphnia			128208		Stenocypris			178742		Crangonyx			212988			Lampsilis			18232		Ceriodaphnia			20676		Hyalella			29972		Brachionus			44693
	Site 13		50		75		85		75428737034162		60327020429132		3000		1300			Daphnia			57284		Ceriodaphnia			125992		Stenocypris			175652		Crangonyx			209307			Lampsilis			17917		Ceriodaphnia			20318		Hyalella			29454		Brachionus			43920
	Site 14		50		100		85		75428737034162		59585352130146		3000		1300			Daphnia			56580		Ceriodaphnia			124443		Stenocypris			173493		Crangonyx			206734			Lampsilis			17696		Ceriodaphnia			20068		Hyalella			29092		Salmo			41264
	Site 15		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 16		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 17		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 18		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 19		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 20		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370

	Appendix C Acceptable Chronic Toxicity Data of Aluminum to Freshwater Aquatic Animals
									MLR equation
	Species		Hardness (mgL as CaCO3)		pH		DOC(mgL)		EC20 Endpoint			EC20(microgL)			Normalized Conc- EC20(microgL)			SMCV-EC20(microgL)		GMCV-EC20(microgL)			Genus			DOC Notes		Dilution water
	Oligochaete Aeolosoma sp		48		6		025		Reproduction (population size)			1235			17098			17098		17098			Aeolosoma		Invert		Author reported half the detection limit
	Rotifer Brachionus calyciflorus		100		62		025		Reproduction (population size)			431			2555			2555		2555			Brachionus		Invert		Author reported half the detection limit
	Great pond snail Lymnaea stagnalis		117		61		025		Biomass			746			4877			4877		4877			Lymnaea		Mollusk		Author reported half the detection limit
	Fatmucket Lampsilis siliquoidea		1055		604		040		Biomass			169			1042			1042		1042			Lampsilis		Mollusk		Author reported
	Cladoceran Ceriodaphnia dubia		50		715		11		Reproduction - youngstarting adult			1780			1898						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		26		73		05		Reproduction - youngfemale			1557			2718						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		46		75		05		Reproduction - youngfemale			8087			1102						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		96		79		05		Reproduction - youngfemale			6519			796						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		194		81		05		Reproduction - youngfemale			6836			888						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		255		703		05		Reproduction (youngfemale)			250			563						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		122		713		05		Reproduction (youngfemale)			860			1061						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		25		798		05		Reproduction (youngfemale)			700			1006						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		61		803		05		Reproduction (youngfemale)			1010			1363						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		122		810		05		Reproduction (youngfemale)			870			1164						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		255		633		05		Reproduction (youngfemale)			260			1758						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		121		636		05		Reproduction (youngfemale)			390			1101			1182		1182			Ceriodaphnia		Invert		Author reported
	Amphipod Hyalella azteca		95		61		051		Biomass (28 d)			1993			1014						Hyalella			Author reported
	Amphipod Hyalella azteca		106		604		033		Biomass (28 d)			425			2895			1713		1713			Hyalella		Invert		Author reported
	Midge Chironomus riparius		118		558		18		Adult midge emergence			2955			1192						Chironomus			Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Midge Chironomus riparius		119		505		18		Adult midge emergence			8442			22578						Chironomus			Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Midge Chironomus riparius		91		66		051		Reproduction ( of eggscase)			3387			7664			5908		5908			Chironomus		Invert		Author reported
	Atlantic salmon Salmo salar		127		57		18		Biomass			6156			5085			5085		5085			Salmo		Fish		Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Brook trout Salvelinus fontinalis		123		655		19		Biomass			1644			270						Salvelinus			Author reported		wellRO mix
	Brook trout Salvelinus fontinalis		128		565		18		Biomass			1435			1295			5914		5914			Salvelinus		Fish		Author reported		wellRO mix
	Fathead minnow Pimephales promelas		220		770		16		Biomass			6194			3569						Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		well water
	Fathead minnow Pimephales promelas		96		620		025		Fry survival			4286			1734			2488		2488			Pimephales		Fish		Author reported half the detection limit
	Zebrafish Danio rerio		83		61		025		Biomass			2344			1102			1102		1102			Danio		Fish		Author reported half the detection limit
	Wood frog Rana sylvatica		115		469		16		MATC-survival		gt		2000		gt		9746		gt		9746		9746		gt		Rana		Amphib - Other Data		Value is from 2007 FW Cu AWQC Appendix C recommendation		aged tap water

	Appendix A Acceptable Acute Toxicity Data of Aluminum to Freshwater Aquatic Animals
	Species		Methoda		Hardness (mgL as CaCO3)		pH		DOC(mgL)			LC50 or EC50 Conc (microgL)		Reference			Normalized Conc - LC50(microgL)			Species Mean Acute Value(microgL)		Genus Mean Acute Value(microgL)			Genus			DOC Note		Dilution water
	Snail Physa sp		S M T		474		659		11		gt		23400		Call 1984 Call et al 1984		gt		48425			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		755		11			30600		Call 1984 Call et al 1984			26986			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		817		11		gt		24700		Call 1984 Call et al 1984		gt		24040			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		746		11			55500		Call 1984 Call et al 1984			50338			35462		35462			Physa		Mollusk		Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Fatmucket Lampsilis siliquoidea		F M T		1060		612		048		gt		6302		Wang et al 2016 Wang et al 2017		gt		29834		gt		29834		29834		gt		Lampsilis		Mollusk		Author reported
	Cladoceran Ceriodaphnia dubia		S M A		500		742		11			1900		McCauley et al 1986			1728			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		S M A		505		786		11			1500		McCauley et al 1986			1294			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		UW-S Lake Superior lab water
	Cladoceran Ceriodaphnia dubia		S M A		500		813		11			2560		McCauley et al 1986			2431			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		R M T		26		75		05			720		ENSR 1992d			1113			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		R M T		46		76		05			1880		ENSR 1992d			2495			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		R M T		96		78		05			2450		ENSR 1992d			2894			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		S U NR		90		715		05			3727		Fort and Stover 1995			5002			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U NR		90		715		05			5673		Fort and Stover 1995			7613			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U NR		89		76		05			2880		Soucek et al 2001			3362			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		601		05			7112		European Al Association 2009			1691			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		605		2			6865		European Al Association 2009			7088			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		609		4			15581		European Al Association 2009			10072			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		601		05			681		European Al Association 2009			1620			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		603		05			1630		European Al Association 2009			3674			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		597		05			1785		European Al Association 2009			4732			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		592		05			1410		European Al Association 2009			4295			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		699		05		gt		1300		European Al Association 2009		gt		4189			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		785		05		gt		5000		European Al Association 2009		gt		7960			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		68		2		gt		10000		European Al Association 2009		gt		20755			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		782		2		gt		15000		European Al Association 2009		gt		11626			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		677		4		gt		10000		European Al Association 2009		gt		15161			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		766		4		gt		15000		European Al Association 2009		gt		8608			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		79		05		gt		2000		European Al Association 2009		gt		3150			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		789		05		gt		2000		European Al Association 2009		gt		3156			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		604		05			1108		European Al Association 2009			850			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		598		2			11371		European Al Association 2009			4817			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		573		4			80467		European Al Association 2009			43570			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		671		05		gt		10000		European Al Association 2009		gt		23786			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		783		05		gt		5000		European Al Association 2009		gt		6344			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		679		2		gt		10000		European Al Association 2009		gt		10394			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		767		2		gt		15000		European Al Association 2009		gt		9082			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		668		4		gt		15000		European Al Association 2009		gt		12461			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		762		4		gt		15000		European Al Association 2009		gt		6332			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		606		2			33868		European Al Association 2009			7920			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		56		4			104842		European Al Association 2009			48800			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		693		05		gt		5000		European Al Association 2009		gt		7126			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		120		788		05		gt		5000		European Al Association 2009		gt		5898			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		120		676		2		gt		15000		European Al Association 2009		gt		12117			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		771		2		gt		15000		European Al Association 2009		gt		8096			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		66		4		gt		15000		European Al Association 2009		gt		10139			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		76		4		gt		15000		European Al Association 2009		gt		5545			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			11971		European Al Association 2010			2699			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			27478		European Al Association 2010			6194			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			11998		European Al Association 2010			2705			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		607		05			92495		European Al Association 2010			1876			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		609		05			31337		European Al Association 2010			6035			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		61		05			33235		European Al Association 2010			6238			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		708		05		gt		8864		European Al Association 2010		gt		2534			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		779		05		gt		42783		European Al Association 2010		gt		6930			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		753		05			13204		European Al Association 2010			244			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		600		601		05			46326		European Al Association 2010			3798			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		600		599		05		gt		8590		European Al Association 2010		gt		7366			5094				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia reticulata		F M T		451		60		11			304		Shephard 1983			2009			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia reticulata		F M T		40		55		11			362		Shephard 1983			55307			10542		7328			Ceriodaphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake SuperiorDI Mix
	Cladoceran Daphnia magna		S U NR		453		774		11			3900		Biesinger and Christensen 1972			3382			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Daphnia magna		S M T		220		76		16			38200		Kimball 1978			20303			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Well water
	Cladoceran Daphnia magna		S U T		451		725		11			2800		Shephard 1983			2868			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Daphnia magna		S U T		168		599		05			5000		European Al Association 2009		gt		2265			-				Daphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Daphnia magna		S U T		168		792		05			7950		European Al Association 2009			920			3332		3332			Daphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Ostracod Stenocypris major		R M T		1563		651		16			310196		Shuhaimi-Othman et al 2011a			10216			10216		10216			Stenocypris		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (Malaysia)
	Amphipod Crangonyx pseudogracilis		R U T		50		675		16			9190		Martin and Holdich 1986			12174			12174		12174			Crangonyx		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (UK)
	Amphipod Hyalella azteca		F M T		105		613		048		gt		5997		Wang et al 2016 Wang et al 2017		gt		28002		gt		28002		28002		gt		Hyalella		Invert		Author reported
	Stonefly Acroneuria sp		S M T		474		746		11		gt		22600		Call et al 1984		gt		20498		gt		20498		20498		gt		Acroneuria		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Midge Chironomus plumosus		S U T		80		70		16			30000		Fargasova 2001			25361			25361		25361			Chironomus		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (Slovakia)
	Midge Paratanytarsus dissimilis		S M T		1743		728		28		gt		77700		Lamb and Bailey 1981 1983		gt		62318		gt		62318		62318		gt		Paratanytarsus		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Liberty Lake Washington
	Rainbow trout Oncorhynchus mykiss		F M T		274		758		05		gt		9840		Gundersen et al 1994		gt		5745			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		452		762		05		gt		8070		Gundersen et al 1994		gt		5212			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		895		758		05		gt		8160		Gundersen et al 1994		gt		6807			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		1304		758		05		gt		8200		Gundersen et al 1994		gt		7662			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		232		825		05			6170		Gundersen et al 1994			1190			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		350		826		05			6170		Gundersen et al 1994			1523			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		836		829		05			7670		Gundersen et al 1994			3205			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		1158		830		05			6930		Gundersen et al 1994			3554			3661		3661			Oncorhynchus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Atlantic salmon Salmo salar		S U T		68		55		05			584		Hamilton and Haines 1995			21042			-				Salmo			Value is from 2007 FW Cu AWQC Appendix C recommendation		Soft recon
	Atlantic salmon Salmo salar		S U T		68		65		05			599		Hamilton and Haines 1995			2430			7151		7151			Salmo		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Soft recon
	Brook trout Salvelinus fontinalis		S U T		40		56		16			6530		Tandjung 1982			31767			-				Salvelinus			Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Brook trout Salvelinus fontinalis		S U T		18		56		16			3400		Tandjung 1982			28391			-				Salvelinus			Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Brook trout Salvelinus fontinalis		S U T		2		56		16			370		Tandjung 1982			13663			23097		23097			Salvelinus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Green sunfish Lepomis cyanellus		S M T		474		755		11		gt		50000		Call et al 1984		gt		24028		gt		24028		24028		gt		Lepomis		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Rio Grande silvery minnow Hybognathus amarus		R M T		140		81		05		gt		59100		Buhl 2002		gt		39414		gt		39414		39414		gt		Hybognathus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon water
	Fathead minnow Pimephales promelas		S M T		474		761		11		gt		48200		Call et al 1984		gt		21522			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Fathead minnow Pimephales promelas		S M T		474		805		11		gt		49800		Call et al 1984		gt		12972			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Fathead minnow Pimephales promelas		R M T		26		78		05			1160		ENSR 1992c			477			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		46		76		05			8180		ENSR 1992c			5446			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		96		81		05			20300		ENSR 1992c			10966			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		194		81		05			44800		ENSR 1992c			35847			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		140		81		05		gt		59100		Buhl 2002		gt		39414			10168		10168			Pimephales		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon water
	Smallmouth bass Micropterus dolomieui		S M T		1245		505		16			130		Kane and Rabeni 1987			3929			3929		3929			Micropterus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Green tree frog Hyla cinerea		R M T		455		549		05		gt		4052		Jung and Jagoe 1995		gt		20016		gt		20016		20016		gt		Hyla		Amphibian		Value is from 2007 FW Cu AWQC Appendix C recommendation		artificial soft recon

	Normalization Chemistry
	pH		7					FAV		2741
	Hardness		100					CMC		1400
	DOC		1					CCC		390
	Acute Ranked GMAV											Chronic Ranked GMCV
	Rank			GMAV		Genus								Rank			GMCV		Genus
	18		gt		62318		Paratanytarsus		Invert							12			17098		Aeolosoma		Invert
	17		gt		39414		Hybognathus		Fish							11		gt		9746		Rana		Amphib - Other Data
	16			35462		Physa		Mollusk							10			5908		Chironomus		Invert
	15		gt		29834		Lampsilis		Mollusk							9			4877		Lymnaea		Mollusk
	14		gt		28002		Hyalella		Invert							8			2555		Brachionus		Invert
	13			25361		Chironomus		Invert							7			2488		Pimephales		Fish
	12		gt		24028		Lepomis		Fish							6			1713		Hyalella		Invert
	11			23097		Salvelinus		Fish							5			1182		Ceriodaphnia		Invert
	10		gt		20498		Acroneuria		Invert							4			1102		Danio		Fish
	9		gt		20016		Hyla		Amphibian							3			1042		Lampsilis		Mollusk
	8			12174		Crangonyx		Invert							2			591		Salvelinus		Fish
	7			10216		Stenocypris		Invert							1			509		Salmo		Fish
	6			10168		Pimephales		Fish
	5			7328		Ceriodaphnia		Invert
	4			7151		Salmo		Fish
	3			3929		Micropterus		Fish
	2			3661		Oncorhynchus		Fish
	1			3332		Daphnia		Invert
		N		Rank		Genus		GMAV		ln(GMAV)		ln(GMAV)2		P=R(N+1)		sqrt(P)				N		Rank		Genus		GMCV		ln(GMCV)		ln(GMCV)2		P=R(N+1)		sqrt(P)
		18		4		Salmo		7151		887		7876		0211		0459				12		4		Danio		1102		700		4907		0308		0555
			3		Micropterus		3929		828		6849		0158		0397					3		Lampsilis		1042		695		4829		0231		0480
			2		Oncorhynchus		3661		821		6733		0105		0324					2		Salvelinus		591		638		4074		0154		0392
			1		Daphnia		3332		811		6579		0053		0229					1		Salmo		509		623		3883		0077		0277
			Sum				3347		2804		0526		141					Sum				2657		1769		0769		170
			S2 =		1223										S2 =		1080
			L =		7134										L =		5242
			A =		7916										A =		5976
			FAV =		2741										FCV =		3940
			CMC =		1371

				All concentrations reported are microgL total Aluminum
				lt-----Click Button after entering data
	ENTER DATA HERE									ERRORNUM		ACUTE														CHRONIC
										Rank 1				Rank 2				Rank 3				Rank 4					Rank 1				Rank 2				Rank 3				Rank 4
	SiteName		DOC(mgL)		Hardness(mgL as CaCO3)		pH			FAV		CMC		CCC			Genus		GMAV			Genus		GMAV			Genus		GMAV			Genus		GMAV				Genus		GMCV			Genus		GMCV			Genus		GMCV			Genus		GMCV
	Site 1		50		25		9		75428737034162		35229324666008		1800		770			Daphnia			33452		Ceriodaphnia			73576		Stenocypris			102576		Crangonyx			122230			Lampsilis			10463		Ceriodaphnia			11865		Hyalella			17200		Brachionus			25648
	Site 2		50		50		9		75428737034162		31204991509063		1600		680			Daphnia			29631		Ceriodaphnia			65171		Stenocypris			90859		Crangonyx			108267			Lampsilis			9268		Ceriodaphnia			10510		Hyalella			15236		Brachionus			22718
	Site 3		50		75		9		75428737034162		29067527928199		1500		640			Daphnia			27601		Ceriodaphnia			60707		Stenocypris			84635		Crangonyx			100851			Lampsilis			8633		Ceriodaphnia			9790		Hyalella			14192		Brachionus			21162
	Site 4		50		100		9		75428737034162		27640367912592		1400		600			Daphnia			26246		Ceriodaphnia			57726		Stenocypris			80480		Crangonyx			95899			Lampsilis			8209		Ceriodaphnia			9309		Hyalella			13495		Brachionus			20123
	Site 5		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 6		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 7		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 8		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 9		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 10		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 11		50		25		85		75428737034162		63245275664384		3200		1400			Daphnia			60055		Ceriodaphnia			132086		Stenocypris			184149		Crangonyx			219432			Lampsilis			18783		Ceriodaphnia			21301		Hyalella			30879		Brachionus			46045
	Site 12		50		50		85		75428737034162		61388044609926		3100		1300			Daphnia			58291		Ceriodaphnia			128208		Stenocypris			178742		Crangonyx			212988			Lampsilis			18232		Ceriodaphnia			20676		Hyalella			29972		Brachionus			44693
	Site 13		50		75		85		75428737034162		60327020429132		3000		1300			Daphnia			57284		Ceriodaphnia			125992		Stenocypris			175652		Crangonyx			209307			Lampsilis			17917		Ceriodaphnia			20318		Hyalella			29454		Brachionus			43920
	Site 14		50		100		85		75428737034162		59585352130146		3000		1300			Daphnia			56580		Ceriodaphnia			124443		Stenocypris			173493		Crangonyx			206734			Lampsilis			17696		Ceriodaphnia			20068		Hyalella			29092		Salmo			41264
	Site 15		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 16		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 17		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 18		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 19		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 20		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370

Aluminum Toxicity Databull The updated freshwater aluminum criteria meets the

acute and chronic minimum data requirements outlined in EPArsquos 1985 Guidelines

InvertebrateSpecies

Fish Species

AmphibianSpecies

Total Genera

Acute1988 AWQC 8 7 - 142017 Draft AWQC 11 8 1 18

Chronic1988 AWQC 2 1 - 32017 Draft AWQC 7 4 - 11

11 1988 AWQC Chronic CCC based on two studies for the striped

bass and brook trout

Aluminum Toxicity Data Freshwater mussels have been

suggested to be potentially sensitive to aluminum

EPA worked collaboratively with USGSrsquos Columbia Environmental Research Center (CERC) to have acute and chronic aluminum toxicity tests conducted with a unionid mussel (fatmucket Lampsilis siliquoidea) in water-only exposures to obtain additional data on invertebrates New data on amphipod (Hyalella azteca)

were collected to confirm laboratory conditions

12

Aluminum Toxicity Data

13

bull New data and models examinedndash Data submitted to EU REACH program

bull New freshwater aluminum toxicity data generated by Oregon State University

bull 2016 BLM model provided by the European Aluminum Association

ndash Saltwater data provided by Australia bull Insufficient data to develop estuarinemarine acute or chronic

criteria at this timendash Multiple Linear Regression (MLR) model developed by

DeForest et al 2017bull DeForest DK KV Brix LM Tear and WJ Adams 2017 Multiple Linear Regression (MLR) models for predicting chronic aluminum

toxicity to freshwater aquatic organisms and developing water quality guidelines Environ Toxicol Chem (DOI101002etc3922)

14

Minimum Dataset For Freshwater Criteria Derivation

SALMONID SECONDFISHFAMILY

CHORDATA

PLANKTONICCRUSTACEAN

BENTHIC CRUSTACEAN

INSECT ROTIFERA ANNELIDA MOLLUSCA

OTHERINSECT ORMOLLUSCA

EstuarineMarine Minimum Data Requirements

15

Family Minimum Data Requirement EstuarineMarine

Acute(Phylum Family Genus)

Chronic(Phylum Family Genus)

Family in the phylum Chordata - -

Family in the phylum Chordata - -

Either the Mysidae or Penaeidae family - -

Family in a phylum other than Arthropoda or Chordata Mollusca Ostreidae Crassostrea -

Family in a phylum other than Chordata Annelida Nereididae Neanthes -

Family in a phylum other than Chordata Annelida Capitellidae Capitella -

Family in a phylum other than Chordata Annelida Ctenodrilidae Ctenodrilus -

Any other family Arthropoda Ameiridae Nitokra -

II Evaluate water quality conditions to determine applicability

bull Criteria are often based on one or more water quality conditions (eg hardness) that affect toxicity of the material to test organisms

bull Aluminum toxicity is highly dependent on the pH of the test waterndash The pH of test waters affects the amount and form

of aluminum that is bioavailable to test speciesndash Hardness and DOC (Dissolved Organic Carbon)

also influence the amount of aluminum that is bioavailable

16

Multiple Bioavailability Modeling Approaches Underwent External Peer Review

17

bull Biotic Ligand Modelsbull Full aluminum BLM (2016 model version) ndash Temperature pH

Dissolved organic carbon (DOC) Major cations (calcium [Ca] magnesium [Mg] sodium [Na] and potassium [K]) Major anions (sulfate [SO4] and chloride [Cl]) Alkalinity and Sulfide

bull Simplified aluminum BLM - pH hardness DOC temperature

bull Multilinear Regression Equationsbull pH and Hardness

Accounting for Effects of Water Chemistry on Bioavailability and Toxicity

bull Water quality criteria can be modified quantitatively when enough data are available to demonstrate that those conditions similarly affect the results with a variety of speciesndash High quality data in the draft aluminum document demonstrated

that pH DOC and hardness affected a variety of species in a similar way when exposed to aluminum concentrations

bull Recent publications by Cardwell et al (2017) and Gensemer et al (2017) summarized short-term aluminum chronic toxicity data across a range of DOC pH and hardness values for three species

18

Multiple Linear Regression (MLR) Models to Account for Effects of Water Chemistry

bull MLR models were developed for each species (DeForest et al 2017)ndash 119862119862 119889119889119906119906119887119887119894119894119886119886 11986411986411986211986220=119890119890[minus41026+[0525timesln(119863119863119874119874119862119862)]+[2201timesln(ℎ119886119886119903119903119889119889)]+(11282times119901119901119867119867)minus(0633times1199011199011198671198672)minus[0264times119901119901119867119867ln(ℎ119886119886119903119903119889119889)]]

ndash 119875119875 119901119901119903119903119900119900119898119898119890119890119897119897119886119886119904119904 11986411986411986211986220=119890119890[minus14029+[0503timesln(119863119863119874119874119862119862)]+[3443timesln(ℎ119886119886119903119903119889119889)]+(3131times119901119901119867119867)minus[0494times119901119901119867119867ln(ℎ119886119886119903119903119889119889)]]

bull These models were applied to both the acute and chronic aluminum toxicity datandash The C dubia model was applied to invertebrate datandash The P promelas model was applied to vertebrate data

19

III Normalize toxicity data in species sensitivity distribution to a set of water

quality conditionsbull Just like single parameter hardness models the MLR

models were used to normalize the aluminum toxicity data to a single set of water quality conditionsndash As an illustration for one set of calculations the draft

aluminum AWQC set these conditions to pH 7 hardness of 100 mgL and DOC of 1 mgL

bull This allows for comparisons to be made between species based on their sensitivity to aluminumndash Apples to apples

20

IV Construct sensitivity distribution to determine criteria at set conditions

21

Figure 8 Ranked Summary of Total Aluminum Genus Mean Acute Values (GMAVs) - Freshwater at pH 7 Hardness of 100 mgL and DOC of 1 mgL

IV Construct sensitivity distribution to determine criteria at set conditions

22

Figure 10 Ranked Summary of Total Aluminum Genus Mean Chronic Values (GMCVs) ndash Freshwater Supplemented with Other Data to Fulfill Missing MDRs at pH 7 Hardness of 100 mgL and DOC of 1 mgL

Aluminum Criteria Calculatorbull The relative ranking of genera in the sensitivity

distribution to which the toxicity data are normalized will change based on the set of water chemistry conditions (ie pH hardness and DOC)

bull To make calculating aluminum criteria easier EPA developed an Aluminum Criteria Calculatorndash Automated so that user only needs to enter water chemistry

conditions of interestndash Allows quick calculations for a variety of conditionsndash Limits calculations to the recommended conditions for which

the MLR models were developed were specified for hardness and DOC

bull No extrapolation beyond hardness and DOC empirical data bounds

23

Aluminum Criteria Calculator SD Example

24

pH=7 Hardness=100 DOC=1 pH=6 Hardness=25 DOC=1

Chronic Ranked GMCVRank GMCV Genus

12 17098 Aeolosoma Invert11 gt 9746 Rana Amphib - Other Data10 5908 Chironomus Invert

9 4877 Lymnaea Mollusk8 2555 Brachionus Invert7 2488 Pimephales Fish6 1713 Hyalella Invert5 1182 Ceriodaphnia Invert4 1102 Danio Fish3 1042 Lampsilis Mollusk2 591 Salvelinus Fish1 509 Salmo Fish

FCV = 3940 microgL total aluminum

Chronic Ranked GMCVRank GMCV Genus

12 gt 2131 Rana Amphib - Other Data11 1710 Aeolosoma Invert10 591 Chironomus Invert

9 544 Pimephales Fish8 488 Lymnaea Mollusk7 255 Brachionus Invert6 241 Danio Fish5 171 Hyalella Invert4 129 Salvelinus Fish3 118 Ceriodaphnia Invert2 111 Salmo Fish1 104 Lampsilis Mollusk

FCV = 987 microgL total aluminum

Multiple Scenarios

Run Scenarios

Summary Sheet

Acute Dataset

Chronic Dataset

Multiple Scenarios

Run Scenarios

Summary Sheet

Acute Dataset

Chronic Dataset

Boundaries of the Criteria Outputsbull MLR models were developed using data that

encompassndash DOC range of 008-5 mgLndash Hardness range of 98-127 mgL (as CaCO3)ndash pH range of 6-81

bull MLR criteria outputs are bounded atndash Maximum of 150 mgL total hardness (as CaCO3)

ndash DOC of 50 mgLndash pH range of the model is 50 to 90

25

Aluminum Criteria Look-Up Tables

26

bull EPA also provided criteria look-up tables in Appendix K

Har

dnes

s

CCC(microgL total aluminum)

(DOC=05 mgL)

pH 50 pH 55 pH 60 pH 65 pH 70 pH 75 pH 80 pH 85 pH 90

25 23 a 14 b 69 c 160 f 350 f 620 i 540 b 410 a 230 a

50 43 a 24 b 100 d 170 f 310 f 630 f 670 j 400 a 200 a

75 61 a 33 b 130 e 180 f 290 f 530 f 730 i 390 a 190 a

100 78 a 41 b 150 e 190 f 280 g 460 f 770 c 390 a 180 a

150 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

200 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

250 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

300 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

350 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

400 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

Aluminum Criteria Look-Up Tables

27

bull EPA also provided criteria look-up tables in Appendix K

Har

dnes

s

CCC(microgL total aluminum)

(DOC=10 mgL)

pH 50 pH 55 pH 60 pH 65 pH 70 pH 75 pH 80 pH 85 pH 90

25 33 a 21 b 99 c 220 f 490 f 900 i 780 b 590 a 330 a

50 61 a 35 b 150 e 250 f 440 f 890 f 960 j 580 a 290 a

75 88 a 47 b 180 e 260 f 410 f 740 f 1100 i 570 a 270 a

100 11 a 59 b 210 e 270 f 390 g 650 f 1100 c 560 b 260 a

150 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

200 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

250 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

300 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

350 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

400 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

Aluminum Criteria Look-Up Tables

28

bull EPA also provided criteria look-up tables in Appendix K

Har

dnes

s

CCC(microgL total aluminum)

(DOC=25 mgL)

pH 50 pH 55 pH 60 pH 65 pH 70 pH 75 pH 80 pH 85 pH 90

25 54 a 34 b 160 c 350 f 770 f 1500 i 1300 b 960 a 540 a

50 99 a 57 b 230 e 390 f 690 f 1400 f 1600 j 930 a 470 a

75 14 a 77 b 290 e 410 f 640 f 1200 f 1700 i 920 a 440 a

100 18 a 96 b 330 e 420 f 620 g 1000 f 1800 d 920 b 420 a

150 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

200 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

250 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

300 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

350 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

400 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

Aluminum Criteria Look-Up Tables

29

bull EPA also provided criteria look-up tables in Appendix K

Har

dnes

s

CCC(microgL total aluminum)

(DOC=50 mgL)

pH 50 pH 55 pH 60 pH 65 pH 70 pH 75 pH 80 pH 85 pH 90

25 77 a 49 b 230 c 500 f 1100 f 2100 c 1900 b 1400 a 770 a

50 14 a 82 b 330 e 540 f 970 f 2000 f 2200 j 1300 a 680 a

75 20 a 110 b 410 e 570 f 900 f 1600 f 2500 i 1300 a 640 a

100 26 a 140 b 460 f 590 f 880 g 1400 f 2500 d 1300 b 600 a

150 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

200 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

250 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

300 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

350 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

400 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

Status

30

bull Draft Document Released to the Federal Register on 72817ndash Public comment period of 60 days ndash Due Date for Comments is 92617

bull Revisions will be made considering public commentsbull Subsequent EPA intra-agency review of final criteria

document will then be conducted bull Publication of final criteria document planned for Late Fall

2017

Federal Register Webpagehttpswwwfederalregistergovdocuments201

707282017-15968request-for-scientific-views-draft-updated-aquatic-life-ambient-water-quality-criteria-for-aluminum

Aluminum Criteria Webpagehttpswwwepagovwqc2017-draft-aquatic-life-

criteria-aluminum-freshwater-documents

31

	Appendix C Acceptable Chronic Toxicity Data of Aluminum to Freshwater Aquatic Animals
									MLR equation
	Species		Hardness (mgL as CaCO3)		pH		DOC(mgL)		EC20 Endpoint			EC20(microgL)			Normalized Conc- EC20(microgL)			SMCV-EC20(microgL)		GMCV-EC20(microgL)			Genus			DOC Notes		Dilution water
	Oligochaete Aeolosoma sp		48		6		025		Reproduction (population size)			1235			1710			1710		1710			Aeolosoma		Invert		Author reported half the detection limit
	Rotifer Brachionus calyciflorus		100		62		025		Reproduction (population size)			431			255			255		255			Brachionus		Invert		Author reported half the detection limit
	Great pond snail Lymnaea stagnalis		117		61		025		Biomass			746			488			488		488			Lymnaea		Mollusk		Author reported half the detection limit
	Fatmucket Lampsilis siliquoidea		1055		604		040		Biomass			169			104			104		104			Lampsilis		Mollusk		Author reported
	Cladoceran Ceriodaphnia dubia		50		715		11		Reproduction - youngstarting adult			1780			190						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		26		73		05		Reproduction - youngfemale			1557			272						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		46		75		05		Reproduction - youngfemale			8087			110						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		96		79		05		Reproduction - youngfemale			6519			80						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		194		81		05		Reproduction - youngfemale			6836			89						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		255		703		05		Reproduction (youngfemale)			250			56						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		122		713		05		Reproduction (youngfemale)			860			106						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		25		798		05		Reproduction (youngfemale)			700			101						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		61		803		05		Reproduction (youngfemale)			1010			136						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		122		810		05		Reproduction (youngfemale)			870			116						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		255		633		05		Reproduction (youngfemale)			260			176						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		121		636		05		Reproduction (youngfemale)			390			110			118		118			Ceriodaphnia		Invert		Author reported
	Amphipod Hyalella azteca		95		61		051		Biomass (28 d)			1993			101						Hyalella			Author reported
	Amphipod Hyalella azteca		106		604		033		Biomass (28 d)			425			289			171		171			Hyalella		Invert		Author reported
	Midge Chironomus riparius		118		558		18		Adult midge emergence			2955			119						Chironomus			Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Midge Chironomus riparius		119		505		18		Adult midge emergence			8442			2258						Chironomus			Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Midge Chironomus riparius		91		66		051		Reproduction ( of eggscase)			3387			766			591		591			Chironomus		Invert		Author reported
	Atlantic salmon Salmo salar		127		57		18		Biomass			6156			1112			1112		1112			Salmo		Fish		Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Brook trout Salvelinus fontinalis		123		655		19		Biomass			1644			59						Salvelinus			Author reported		wellRO mix
	Brook trout Salvelinus fontinalis		128		565		18		Biomass			1435			283			1293		1293			Salvelinus		Fish		Author reported		wellRO mix
	Fathead minnow Pimephales promelas		220		770		16		Biomass			6194			781						Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		well water
	Fathead minnow Pimephales promelas		96		620		025		Fry survival			4286			379			544		544			Pimephales		Fish		Author reported half the detection limit
	Zebrafish Danio rerio		83		61		025		Biomass			2344			241			241		241			Danio		Fish		Author reported half the detection limit
	Wood frog Rana sylvatica		115		469		16		MATC-survival		gt		2000		gt		2131		gt		2131		2131		gt		Rana		Amphib - Other Data		Value is from 2007 FW Cu AWQC Appendix C recommendation		aged tap water

	Appendix A Acceptable Acute Toxicity Data of Aluminum to Freshwater Aquatic Animals
	Species		Methoda		Hardness (mgL as CaCO3)		pH		DOC(mgL)			LC50 or EC50 Conc (microgL)		Reference			Normalized Conc - LC50(microgL)			Species Mean Acute Value(microgL)		Genus Mean Acute Value(microgL)			Genus			DOC Note		Dilution water
	Snail Physa sp		S M T		474		659		11		gt		23400		Call 1984 Call et al 1984		gt		4843			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		755		11			30600		Call 1984 Call et al 1984			2699			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		817		11		gt		24700		Call 1984 Call et al 1984		gt		2404			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		746		11			55500		Call 1984 Call et al 1984			5034			3546		3546			Physa		Mollusk		Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Fatmucket Lampsilis siliquoidea		F M T		1060		612		048		gt		6302		Wang et al 2016 Wang et al 2017		gt		2983		gt		2983		2983		gt		Lampsilis		Mollusk		Author reported
	Cladoceran Ceriodaphnia dubia		S M A		500		742		11			1900		McCauley et al 1986			173			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		S M A		505		786		11			1500		McCauley et al 1986			129			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		UW-S Lake Superior lab water
	Cladoceran Ceriodaphnia dubia		S M A		500		813		11			2560		McCauley et al 1986			243			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		R M T		26		75		05			720		ENSR 1992d			111			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		R M T		46		76		05			1880		ENSR 1992d			250			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		R M T		96		78		05			2450		ENSR 1992d			289			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		S U NR		90		715		05			3727		Fort and Stover 1995			500			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U NR		90		715		05			5673		Fort and Stover 1995			761			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U NR		89		76		05			2880		Soucek et al 2001			336			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		601		05			7112		European Al Association 2009			169			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		605		2			6865		European Al Association 2009			709			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		609		4			15581		European Al Association 2009			1007			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		601		05			681		European Al Association 2009			162			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		603		05			1630		European Al Association 2009			367			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		597		05			1785		European Al Association 2009			473			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		592		05			1410		European Al Association 2009			429			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		699		05		gt		1300		European Al Association 2009		gt		419			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		785		05		gt		5000		European Al Association 2009		gt		796			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		68		2		gt		10000		European Al Association 2009		gt		2076			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		782		2		gt		15000		European Al Association 2009		gt		1163			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		677		4		gt		10000		European Al Association 2009		gt		1516			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		766		4		gt		15000		European Al Association 2009		gt		861			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		79		05		gt		2000		European Al Association 2009		gt		315			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		789		05		gt		2000		European Al Association 2009		gt		316			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		604		05			1108		European Al Association 2009			85			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		598		2			11371		European Al Association 2009			482			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		573		4			80467		European Al Association 2009			4357			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		671		05		gt		10000		European Al Association 2009		gt		2379			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		783		05		gt		5000		European Al Association 2009		gt		634			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		679		2		gt		10000		European Al Association 2009		gt		1039			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		767		2		gt		15000		European Al Association 2009		gt		908			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		668		4		gt		15000		European Al Association 2009		gt		1246			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		762		4		gt		15000		European Al Association 2009		gt		633			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		606		2			33868		European Al Association 2009			792			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		56		4			104842		European Al Association 2009			4880			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		693		05		gt		5000		European Al Association 2009		gt		713			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		120		788		05		gt		5000		European Al Association 2009		gt		590			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		120		676		2		gt		15000		European Al Association 2009		gt		1212			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		771		2		gt		15000		European Al Association 2009		gt		810			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		66		4		gt		15000		European Al Association 2009		gt		1014			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		76		4		gt		15000		European Al Association 2009		gt		555			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			11971		European Al Association 2010			270			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			27478		European Al Association 2010			619			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			11998		European Al Association 2010			270			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		607		05			92495		European Al Association 2010			188			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		609		05			31337		European Al Association 2010			604			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		61		05			33235		European Al Association 2010			624			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		708		05		gt		8864		European Al Association 2010		gt		253			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		779		05		gt		42783		European Al Association 2010		gt		693			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		753		05			13204		European Al Association 2010			24			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		600		601		05			46326		European Al Association 2010			380			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		600		599		05		gt		8590		European Al Association 2010		gt		737			509				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia reticulata		F M T		451		60		11			304		Shephard 1983			201			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia reticulata		F M T		40		55		11			362		Shephard 1983			5531			1054		733			Ceriodaphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake SuperiorDI Mix
	Cladoceran Daphnia magna		S U NR		453		774		11			3900		Biesinger and Christensen 1972			338			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Daphnia magna		S M T		220		76		16			38200		Kimball 1978			2030			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Well water
	Cladoceran Daphnia magna		S U T		451		725		11			2800		Shephard 1983			287			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Daphnia magna		S U T		168		599		05			5000		European Al Association 2009		gt		227			-				Daphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Daphnia magna		S U T		168		792		05			7950		European Al Association 2009			92			333		333			Daphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Ostracod Stenocypris major		R M T		1563		651		16			310196		Shuhaimi-Othman et al 2011a			1022			1022		1022			Stenocypris		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (Malaysia)
	Amphipod Crangonyx pseudogracilis		R U T		50		675		16			9190		Martin and Holdich 1986			1217			1217		1217			Crangonyx		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (UK)
	Amphipod Hyalella azteca		F M T		105		613		048		gt		5997		Wang et al 2016 Wang et al 2017		gt		2800		gt		2800		2800		gt		Hyalella		Invert		Author reported
	Stonefly Acroneuria sp		S M T		474		746		11		gt		22600		Call et al 1984		gt		2050		gt		2050		2050		gt		Acroneuria		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Midge Chironomus plumosus		S U T		80		70		16			30000		Fargasova 2001			2536			2536		2536			Chironomus		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (Slovakia)
	Midge Paratanytarsus dissimilis		S M T		1743		728		28		gt		77700		Lamb and Bailey 1981 1983		gt		6232		gt		6232		6232		gt		Paratanytarsus		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Liberty Lake Washington
	Rainbow trout Oncorhynchus mykiss		F M T		274		758		05		gt		9840		Gundersen et al 1994		gt		1256			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		452		762		05		gt		8070		Gundersen et al 1994		gt		1140			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		895		758		05		gt		8160		Gundersen et al 1994		gt		1489			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		1304		758		05		gt		8200		Gundersen et al 1994		gt		1676			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		232		825		05			6170		Gundersen et al 1994			260			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		350		826		05			6170		Gundersen et al 1994			333			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		836		829		05			7670		Gundersen et al 1994			701			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		1158		830		05			6930		Gundersen et al 1994			777			801		801			Oncorhynchus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Atlantic salmon Salmo salar		S U T		68		55		05			584		Hamilton and Haines 1995			4602			-				Salmo			Value is from 2007 FW Cu AWQC Appendix C recommendation		Soft recon
	Atlantic salmon Salmo salar		S U T		68		65		05			599		Hamilton and Haines 1995			531			1564		1564			Salmo		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Soft recon
	Brook trout Salvelinus fontinalis		S U T		40		56		16			6530		Tandjung 1982			6947			-				Salvelinus			Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Brook trout Salvelinus fontinalis		S U T		18		56		16			3400		Tandjung 1982			6209			-				Salvelinus			Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Brook trout Salvelinus fontinalis		S U T		2		56		16			370		Tandjung 1982			2988			5051		5051			Salvelinus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Green sunfish Lepomis cyanellus		S M T		474		755		11		gt		50000		Call et al 1984		gt		5255		gt		5255		5255		gt		Lepomis		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Rio Grande silvery minnow Hybognathus amarus		R M T		140		81		05		gt		59100		Buhl 2002		gt		8620		gt		8620		8620		gt		Hybognathus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon water
	Fathead minnow Pimephales promelas		S M T		474		761		11		gt		48200		Call et al 1984		gt		4707			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Fathead minnow Pimephales promelas		S M T		474		805		11		gt		49800		Call et al 1984		gt		2837			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Fathead minnow Pimephales promelas		R M T		26		78		05			1160		ENSR 1992c			104			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		46		76		05			8180		ENSR 1992c			1191			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		96		81		05			20300		ENSR 1992c			2398			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		194		81		05			44800		ENSR 1992c			7839			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		140		81		05		gt		59100		Buhl 2002		gt		8620			2224		2224			Pimephales		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon water
	Smallmouth bass Micropterus dolomieui		S M T		1245		505		16			130		Kane and Rabeni 1987			859			859		859			Micropterus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Green tree frog Hyla cinerea		R M T		455		549		05		gt		4052		Jung and Jagoe 1995		gt		4377		gt		4377		4377		gt		Hyla		Amphibian		Value is from 2007 FW Cu AWQC Appendix C recommendation		artificial soft recon

	Normalization Chemistry
	pH		6					FAV		360
	Hardness		25					CMC		180
	DOC		1					CCC		99
	Acute Ranked GMAV											Chronic Ranked GMCV
	Rank			GMAV		Genus								Rank			GMCV		Genus
	18		gt		8620		Hybognathus		Fish							12		gt		2131		Rana		Amphib - Other Data
	17		gt		6232		Paratanytarsus		Invert							11			1710		Aeolosoma		Invert
	16		gt		5255		Lepomis		Fish							10			591		Chironomus		Invert
	15			5051		Salvelinus		Fish							9			544		Pimephales		Fish
	14		gt		4377		Hyla		Amphibian							8			488		Lymnaea		Mollusk
	13			3546		Physa		Mollusk							7			255		Brachionus		Invert
	12		gt		2983		Lampsilis		Mollusk							6			241		Danio		Fish
	11		gt		2800		Hyalella		Invert							5			171		Hyalella		Invert
	10			2536		Chironomus		Invert							4			129		Salvelinus		Fish
	9			2224		Pimephales		Fish							3			118		Ceriodaphnia		Invert
	8		gt		2050		Acroneuria		Invert							2			111		Salmo		Fish
	7			1564		Salmo		Fish							1			104		Lampsilis		Mollusk
	6			1217		Crangonyx		Invert
	5			1022		Stenocypris		Invert
	4			859		Micropterus		Fish
	3			801		Oncorhynchus		Fish
	2			733		Ceriodaphnia		Invert
	1			333		Daphnia		Invert
		N		Rank		Genus		GMAV		ln(GMAV)		ln(GMAV)2		P=R(N+1)		sqrt(P)				N		Rank		Genus		GMCV		ln(GMCV)		ln(GMCV)2		P=R(N+1)		sqrt(P)
		18		4		Micropterus		859		676		4564		0211		0459				12		4		Salvelinus		129		486		2364		0308		0555
			3		Oncorhynchus		801		669		4469		0158		0397					3		Ceriodaphnia		118		477		2277		0231		0480
			2		Ceriodaphnia		733		660		4352		0105		0324					2		Salmo		111		471		2220		0154		0392
			1		Daphnia		333		581		3374		0053		0229					1		Lampsilis		104		465		2159		0077		0277
			Sum				2585		1676		0526		141					Sum				1899		902		0769		170
			S2 =		1987										S2 =		059
			L =		4890										L =		4420
			A =		5887										A =		4592
			FAV =		360										FCV =		987
			CMC =		180

				All concentrations reported are microgL total Aluminum
				lt-----Click Button after entering data
	ENTER DATA HERE									ERRORNUM		ACUTE														CHRONIC
										Rank 1				Rank 2				Rank 3				Rank 4					Rank 1				Rank 2				Rank 3				Rank 4
	SiteName		DOC(mgL)		Hardness(mgL as CaCO3)		pH			FAV		CMC		CCC			Genus		GMAV			Genus		GMAV			Genus		GMAV			Genus		GMAV				Genus		GMCV			Genus		GMCV			Genus		GMCV			Genus		GMCV
	Site 1		50		25		9		75428737034162		35229324666008		1800		770			Daphnia			33452		Ceriodaphnia			73576		Stenocypris			102576		Crangonyx			122230			Lampsilis			10463		Ceriodaphnia			11865		Hyalella			17200		Brachionus			25648
	Site 2		50		50		9		75428737034162		31204991509063		1600		680			Daphnia			29631		Ceriodaphnia			65171		Stenocypris			90859		Crangonyx			108267			Lampsilis			9268		Ceriodaphnia			10510		Hyalella			15236		Brachionus			22718
	Site 3		50		75		9		75428737034162		29067527928199		1500		640			Daphnia			27601		Ceriodaphnia			60707		Stenocypris			84635		Crangonyx			100851			Lampsilis			8633		Ceriodaphnia			9790		Hyalella			14192		Brachionus			21162
	Site 4		50		100		9		75428737034162		27640367912592		1400		600			Daphnia			26246		Ceriodaphnia			57726		Stenocypris			80480		Crangonyx			95899			Lampsilis			8209		Ceriodaphnia			9309		Hyalella			13495		Brachionus			20123
	Site 5		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 6		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 7		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 8		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 9		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 10		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 11		50		25		85		75428737034162		63245275664384		3200		1400			Daphnia			60055		Ceriodaphnia			132086		Stenocypris			184149		Crangonyx			219432			Lampsilis			18783		Ceriodaphnia			21301		Hyalella			30879		Brachionus			46045
	Site 12		50		50		85		75428737034162		61388044609926		3100		1300			Daphnia			58291		Ceriodaphnia			128208		Stenocypris			178742		Crangonyx			212988			Lampsilis			18232		Ceriodaphnia			20676		Hyalella			29972		Brachionus			44693
	Site 13		50		75		85		75428737034162		60327020429132		3000		1300			Daphnia			57284		Ceriodaphnia			125992		Stenocypris			175652		Crangonyx			209307			Lampsilis			17917		Ceriodaphnia			20318		Hyalella			29454		Brachionus			43920
	Site 14		50		100		85		75428737034162		59585352130146		3000		1300			Daphnia			56580		Ceriodaphnia			124443		Stenocypris			173493		Crangonyx			206734			Lampsilis			17696		Ceriodaphnia			20068		Hyalella			29092		Salmo			41264
	Site 15		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 16		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 17		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 18		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 19		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 20		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370

	Appendix C Acceptable Chronic Toxicity Data of Aluminum to Freshwater Aquatic Animals
									MLR equation
	Species		Hardness (mgL as CaCO3)		pH		DOC(mgL)		EC20 Endpoint			EC20(microgL)			Normalized Conc- EC20(microgL)			SMCV-EC20(microgL)		GMCV-EC20(microgL)			Genus			DOC Notes		Dilution water
	Oligochaete Aeolosoma sp		48		6		025		Reproduction (population size)			1235			17098			17098		17098			Aeolosoma		Invert		Author reported half the detection limit
	Rotifer Brachionus calyciflorus		100		62		025		Reproduction (population size)			431			2555			2555		2555			Brachionus		Invert		Author reported half the detection limit
	Great pond snail Lymnaea stagnalis		117		61		025		Biomass			746			4877			4877		4877			Lymnaea		Mollusk		Author reported half the detection limit
	Fatmucket Lampsilis siliquoidea		1055		604		040		Biomass			169			1042			1042		1042			Lampsilis		Mollusk		Author reported
	Cladoceran Ceriodaphnia dubia		50		715		11		Reproduction - youngstarting adult			1780			1898						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		26		73		05		Reproduction - youngfemale			1557			2718						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		46		75		05		Reproduction - youngfemale			8087			1102						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		96		79		05		Reproduction - youngfemale			6519			796						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		194		81		05		Reproduction - youngfemale			6836			888						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		255		703		05		Reproduction (youngfemale)			250			563						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		122		713		05		Reproduction (youngfemale)			860			1061						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		25		798		05		Reproduction (youngfemale)			700			1006						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		61		803		05		Reproduction (youngfemale)			1010			1363						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		122		810		05		Reproduction (youngfemale)			870			1164						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		255		633		05		Reproduction (youngfemale)			260			1758						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		121		636		05		Reproduction (youngfemale)			390			1101			1182		1182			Ceriodaphnia		Invert		Author reported
	Amphipod Hyalella azteca		95		61		051		Biomass (28 d)			1993			1014						Hyalella			Author reported
	Amphipod Hyalella azteca		106		604		033		Biomass (28 d)			425			2895			1713		1713			Hyalella		Invert		Author reported
	Midge Chironomus riparius		118		558		18		Adult midge emergence			2955			1192						Chironomus			Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Midge Chironomus riparius		119		505		18		Adult midge emergence			8442			22578						Chironomus			Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Midge Chironomus riparius		91		66		051		Reproduction ( of eggscase)			3387			7664			5908		5908			Chironomus		Invert		Author reported
	Atlantic salmon Salmo salar		127		57		18		Biomass			6156			5085			5085		5085			Salmo		Fish		Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Brook trout Salvelinus fontinalis		123		655		19		Biomass			1644			270						Salvelinus			Author reported		wellRO mix
	Brook trout Salvelinus fontinalis		128		565		18		Biomass			1435			1295			5914		5914			Salvelinus		Fish		Author reported		wellRO mix
	Fathead minnow Pimephales promelas		220		770		16		Biomass			6194			3569						Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		well water
	Fathead minnow Pimephales promelas		96		620		025		Fry survival			4286			1734			2488		2488			Pimephales		Fish		Author reported half the detection limit
	Zebrafish Danio rerio		83		61		025		Biomass			2344			1102			1102		1102			Danio		Fish		Author reported half the detection limit
	Wood frog Rana sylvatica		115		469		16		MATC-survival		gt		2000		gt		9746		gt		9746		9746		gt		Rana		Amphib - Other Data		Value is from 2007 FW Cu AWQC Appendix C recommendation		aged tap water

	Appendix A Acceptable Acute Toxicity Data of Aluminum to Freshwater Aquatic Animals
	Species		Methoda		Hardness (mgL as CaCO3)		pH		DOC(mgL)			LC50 or EC50 Conc (microgL)		Reference			Normalized Conc - LC50(microgL)			Species Mean Acute Value(microgL)		Genus Mean Acute Value(microgL)			Genus			DOC Note		Dilution water
	Snail Physa sp		S M T		474		659		11		gt		23400		Call 1984 Call et al 1984		gt		48425			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		755		11			30600		Call 1984 Call et al 1984			26986			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		817		11		gt		24700		Call 1984 Call et al 1984		gt		24040			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		746		11			55500		Call 1984 Call et al 1984			50338			35462		35462			Physa		Mollusk		Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Fatmucket Lampsilis siliquoidea		F M T		1060		612		048		gt		6302		Wang et al 2016 Wang et al 2017		gt		29834		gt		29834		29834		gt		Lampsilis		Mollusk		Author reported
	Cladoceran Ceriodaphnia dubia		S M A		500		742		11			1900		McCauley et al 1986			1728			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		S M A		505		786		11			1500		McCauley et al 1986			1294			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		UW-S Lake Superior lab water
	Cladoceran Ceriodaphnia dubia		S M A		500		813		11			2560		McCauley et al 1986			2431			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		R M T		26		75		05			720		ENSR 1992d			1113			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		R M T		46		76		05			1880		ENSR 1992d			2495			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		R M T		96		78		05			2450		ENSR 1992d			2894			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		S U NR		90		715		05			3727		Fort and Stover 1995			5002			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U NR		90		715		05			5673		Fort and Stover 1995			7613			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U NR		89		76		05			2880		Soucek et al 2001			3362			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		601		05			7112		European Al Association 2009			1691			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		605		2			6865		European Al Association 2009			7088			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		609		4			15581		European Al Association 2009			10072			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		601		05			681		European Al Association 2009			1620			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		603		05			1630		European Al Association 2009			3674			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		597		05			1785		European Al Association 2009			4732			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		592		05			1410		European Al Association 2009			4295			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		699		05		gt		1300		European Al Association 2009		gt		4189			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		785		05		gt		5000		European Al Association 2009		gt		7960			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		68		2		gt		10000		European Al Association 2009		gt		20755			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		782		2		gt		15000		European Al Association 2009		gt		11626			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		677		4		gt		10000		European Al Association 2009		gt		15161			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		766		4		gt		15000		European Al Association 2009		gt		8608			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		79		05		gt		2000		European Al Association 2009		gt		3150			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		789		05		gt		2000		European Al Association 2009		gt		3156			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		604		05			1108		European Al Association 2009			850			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		598		2			11371		European Al Association 2009			4817			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		573		4			80467		European Al Association 2009			43570			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		671		05		gt		10000		European Al Association 2009		gt		23786			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		783		05		gt		5000		European Al Association 2009		gt		6344			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		679		2		gt		10000		European Al Association 2009		gt		10394			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		767		2		gt		15000		European Al Association 2009		gt		9082			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		668		4		gt		15000		European Al Association 2009		gt		12461			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		762		4		gt		15000		European Al Association 2009		gt		6332			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		606		2			33868		European Al Association 2009			7920			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		56		4			104842		European Al Association 2009			48800			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		693		05		gt		5000		European Al Association 2009		gt		7126			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		120		788		05		gt		5000		European Al Association 2009		gt		5898			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		120		676		2		gt		15000		European Al Association 2009		gt		12117			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		771		2		gt		15000		European Al Association 2009		gt		8096			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		66		4		gt		15000		European Al Association 2009		gt		10139			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		76		4		gt		15000		European Al Association 2009		gt		5545			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			11971		European Al Association 2010			2699			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			27478		European Al Association 2010			6194			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			11998		European Al Association 2010			2705			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		607		05			92495		European Al Association 2010			1876			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		609		05			31337		European Al Association 2010			6035			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		61		05			33235		European Al Association 2010			6238			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		708		05		gt		8864		European Al Association 2010		gt		2534			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		779		05		gt		42783		European Al Association 2010		gt		6930			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		753		05			13204		European Al Association 2010			244			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		600		601		05			46326		European Al Association 2010			3798			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		600		599		05		gt		8590		European Al Association 2010		gt		7366			5094				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia reticulata		F M T		451		60		11			304		Shephard 1983			2009			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia reticulata		F M T		40		55		11			362		Shephard 1983			55307			10542		7328			Ceriodaphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake SuperiorDI Mix
	Cladoceran Daphnia magna		S U NR		453		774		11			3900		Biesinger and Christensen 1972			3382			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Daphnia magna		S M T		220		76		16			38200		Kimball 1978			20303			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Well water
	Cladoceran Daphnia magna		S U T		451		725		11			2800		Shephard 1983			2868			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Daphnia magna		S U T		168		599		05			5000		European Al Association 2009		gt		2265			-				Daphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Daphnia magna		S U T		168		792		05			7950		European Al Association 2009			920			3332		3332			Daphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Ostracod Stenocypris major		R M T		1563		651		16			310196		Shuhaimi-Othman et al 2011a			10216			10216		10216			Stenocypris		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (Malaysia)
	Amphipod Crangonyx pseudogracilis		R U T		50		675		16			9190		Martin and Holdich 1986			12174			12174		12174			Crangonyx		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (UK)
	Amphipod Hyalella azteca		F M T		105		613		048		gt		5997		Wang et al 2016 Wang et al 2017		gt		28002		gt		28002		28002		gt		Hyalella		Invert		Author reported
	Stonefly Acroneuria sp		S M T		474		746		11		gt		22600		Call et al 1984		gt		20498		gt		20498		20498		gt		Acroneuria		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Midge Chironomus plumosus		S U T		80		70		16			30000		Fargasova 2001			25361			25361		25361			Chironomus		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (Slovakia)
	Midge Paratanytarsus dissimilis		S M T		1743		728		28		gt		77700		Lamb and Bailey 1981 1983		gt		62318		gt		62318		62318		gt		Paratanytarsus		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Liberty Lake Washington
	Rainbow trout Oncorhynchus mykiss		F M T		274		758		05		gt		9840		Gundersen et al 1994		gt		5745			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		452		762		05		gt		8070		Gundersen et al 1994		gt		5212			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		895		758		05		gt		8160		Gundersen et al 1994		gt		6807			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		1304		758		05		gt		8200		Gundersen et al 1994		gt		7662			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		232		825		05			6170		Gundersen et al 1994			1190			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		350		826		05			6170		Gundersen et al 1994			1523			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		836		829		05			7670		Gundersen et al 1994			3205			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		1158		830		05			6930		Gundersen et al 1994			3554			3661		3661			Oncorhynchus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Atlantic salmon Salmo salar		S U T		68		55		05			584		Hamilton and Haines 1995			21042			-				Salmo			Value is from 2007 FW Cu AWQC Appendix C recommendation		Soft recon
	Atlantic salmon Salmo salar		S U T		68		65		05			599		Hamilton and Haines 1995			2430			7151		7151			Salmo		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Soft recon
	Brook trout Salvelinus fontinalis		S U T		40		56		16			6530		Tandjung 1982			31767			-				Salvelinus			Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Brook trout Salvelinus fontinalis		S U T		18		56		16			3400		Tandjung 1982			28391			-				Salvelinus			Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Brook trout Salvelinus fontinalis		S U T		2		56		16			370		Tandjung 1982			13663			23097		23097			Salvelinus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Green sunfish Lepomis cyanellus		S M T		474		755		11		gt		50000		Call et al 1984		gt		24028		gt		24028		24028		gt		Lepomis		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Rio Grande silvery minnow Hybognathus amarus		R M T		140		81		05		gt		59100		Buhl 2002		gt		39414		gt		39414		39414		gt		Hybognathus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon water
	Fathead minnow Pimephales promelas		S M T		474		761		11		gt		48200		Call et al 1984		gt		21522			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Fathead minnow Pimephales promelas		S M T		474		805		11		gt		49800		Call et al 1984		gt		12972			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Fathead minnow Pimephales promelas		R M T		26		78		05			1160		ENSR 1992c			477			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		46		76		05			8180		ENSR 1992c			5446			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		96		81		05			20300		ENSR 1992c			10966			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		194		81		05			44800		ENSR 1992c			35847			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		140		81		05		gt		59100		Buhl 2002		gt		39414			10168		10168			Pimephales		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon water
	Smallmouth bass Micropterus dolomieui		S M T		1245		505		16			130		Kane and Rabeni 1987			3929			3929		3929			Micropterus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Green tree frog Hyla cinerea		R M T		455		549		05		gt		4052		Jung and Jagoe 1995		gt		20016		gt		20016		20016		gt		Hyla		Amphibian		Value is from 2007 FW Cu AWQC Appendix C recommendation		artificial soft recon

	Normalization Chemistry
	pH		7					FAV		2741
	Hardness		100					CMC		1400
	DOC		1					CCC		390
	Acute Ranked GMAV											Chronic Ranked GMCV
	Rank			GMAV		Genus								Rank			GMCV		Genus
	18		gt		62318		Paratanytarsus		Invert							12			17098		Aeolosoma		Invert
	17		gt		39414		Hybognathus		Fish							11		gt		9746		Rana		Amphib - Other Data
	16			35462		Physa		Mollusk							10			5908		Chironomus		Invert
	15		gt		29834		Lampsilis		Mollusk							9			4877		Lymnaea		Mollusk
	14		gt		28002		Hyalella		Invert							8			2555		Brachionus		Invert
	13			25361		Chironomus		Invert							7			2488		Pimephales		Fish
	12		gt		24028		Lepomis		Fish							6			1713		Hyalella		Invert
	11			23097		Salvelinus		Fish							5			1182		Ceriodaphnia		Invert
	10		gt		20498		Acroneuria		Invert							4			1102		Danio		Fish
	9		gt		20016		Hyla		Amphibian							3			1042		Lampsilis		Mollusk
	8			12174		Crangonyx		Invert							2			591		Salvelinus		Fish
	7			10216		Stenocypris		Invert							1			509		Salmo		Fish
	6			10168		Pimephales		Fish
	5			7328		Ceriodaphnia		Invert
	4			7151		Salmo		Fish
	3			3929		Micropterus		Fish
	2			3661		Oncorhynchus		Fish
	1			3332		Daphnia		Invert
		N		Rank		Genus		GMAV		ln(GMAV)		ln(GMAV)2		P=R(N+1)		sqrt(P)				N		Rank		Genus		GMCV		ln(GMCV)		ln(GMCV)2		P=R(N+1)		sqrt(P)
		18		4		Salmo		7151		887		7876		0211		0459				12		4		Danio		1102		700		4907		0308		0555
			3		Micropterus		3929		828		6849		0158		0397					3		Lampsilis		1042		695		4829		0231		0480
			2		Oncorhynchus		3661		821		6733		0105		0324					2		Salvelinus		591		638		4074		0154		0392
			1		Daphnia		3332		811		6579		0053		0229					1		Salmo		509		623		3883		0077		0277
			Sum				3347		2804		0526		141					Sum				2657		1769		0769		170
			S2 =		1223										S2 =		1080
			L =		7134										L =		5242
			A =		7916										A =		5976
			FAV =		2741										FCV =		3940
			CMC =		1371

				All concentrations reported are microgL total Aluminum
				lt-----Click Button after entering data
	ENTER DATA HERE									ERRORNUM		ACUTE														CHRONIC
										Rank 1				Rank 2				Rank 3				Rank 4					Rank 1				Rank 2				Rank 3				Rank 4
	SiteName		DOC(mgL)		Hardness(mgL as CaCO3)		pH			FAV		CMC		CCC			Genus		GMAV			Genus		GMAV			Genus		GMAV			Genus		GMAV				Genus		GMCV			Genus		GMCV			Genus		GMCV			Genus		GMCV
	Site 1		50		25		9		75428737034162		35229324666008		1800		770			Daphnia			33452		Ceriodaphnia			73576		Stenocypris			102576		Crangonyx			122230			Lampsilis			10463		Ceriodaphnia			11865		Hyalella			17200		Brachionus			25648
	Site 2		50		50		9		75428737034162		31204991509063		1600		680			Daphnia			29631		Ceriodaphnia			65171		Stenocypris			90859		Crangonyx			108267			Lampsilis			9268		Ceriodaphnia			10510		Hyalella			15236		Brachionus			22718
	Site 3		50		75		9		75428737034162		29067527928199		1500		640			Daphnia			27601		Ceriodaphnia			60707		Stenocypris			84635		Crangonyx			100851			Lampsilis			8633		Ceriodaphnia			9790		Hyalella			14192		Brachionus			21162
	Site 4		50		100		9		75428737034162		27640367912592		1400		600			Daphnia			26246		Ceriodaphnia			57726		Stenocypris			80480		Crangonyx			95899			Lampsilis			8209		Ceriodaphnia			9309		Hyalella			13495		Brachionus			20123
	Site 5		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 6		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 7		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 8		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 9		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 10		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 11		50		25		85		75428737034162		63245275664384		3200		1400			Daphnia			60055		Ceriodaphnia			132086		Stenocypris			184149		Crangonyx			219432			Lampsilis			18783		Ceriodaphnia			21301		Hyalella			30879		Brachionus			46045
	Site 12		50		50		85		75428737034162		61388044609926		3100		1300			Daphnia			58291		Ceriodaphnia			128208		Stenocypris			178742		Crangonyx			212988			Lampsilis			18232		Ceriodaphnia			20676		Hyalella			29972		Brachionus			44693
	Site 13		50		75		85		75428737034162		60327020429132		3000		1300			Daphnia			57284		Ceriodaphnia			125992		Stenocypris			175652		Crangonyx			209307			Lampsilis			17917		Ceriodaphnia			20318		Hyalella			29454		Brachionus			43920
	Site 14		50		100		85		75428737034162		59585352130146		3000		1300			Daphnia			56580		Ceriodaphnia			124443		Stenocypris			173493		Crangonyx			206734			Lampsilis			17696		Ceriodaphnia			20068		Hyalella			29092		Salmo			41264
	Site 15		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 16		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 17		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 18		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 19		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 20		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370

Aluminum Toxicity Data Freshwater mussels have been

suggested to be potentially sensitive to aluminum

EPA worked collaboratively with USGSrsquos Columbia Environmental Research Center (CERC) to have acute and chronic aluminum toxicity tests conducted with a unionid mussel (fatmucket Lampsilis siliquoidea) in water-only exposures to obtain additional data on invertebrates New data on amphipod (Hyalella azteca)

were collected to confirm laboratory conditions

12

Aluminum Toxicity Data

13

bull New data and models examinedndash Data submitted to EU REACH program

bull New freshwater aluminum toxicity data generated by Oregon State University

bull 2016 BLM model provided by the European Aluminum Association

ndash Saltwater data provided by Australia bull Insufficient data to develop estuarinemarine acute or chronic

criteria at this timendash Multiple Linear Regression (MLR) model developed by

DeForest et al 2017bull DeForest DK KV Brix LM Tear and WJ Adams 2017 Multiple Linear Regression (MLR) models for predicting chronic aluminum

toxicity to freshwater aquatic organisms and developing water quality guidelines Environ Toxicol Chem (DOI101002etc3922)

14

Minimum Dataset For Freshwater Criteria Derivation

SALMONID SECONDFISHFAMILY

CHORDATA

PLANKTONICCRUSTACEAN

BENTHIC CRUSTACEAN

INSECT ROTIFERA ANNELIDA MOLLUSCA

OTHERINSECT ORMOLLUSCA

EstuarineMarine Minimum Data Requirements

15

Family Minimum Data Requirement EstuarineMarine

Acute(Phylum Family Genus)

Chronic(Phylum Family Genus)

Family in the phylum Chordata - -

Family in the phylum Chordata - -

Either the Mysidae or Penaeidae family - -

Family in a phylum other than Arthropoda or Chordata Mollusca Ostreidae Crassostrea -

Family in a phylum other than Chordata Annelida Nereididae Neanthes -

Family in a phylum other than Chordata Annelida Capitellidae Capitella -

Family in a phylum other than Chordata Annelida Ctenodrilidae Ctenodrilus -

Any other family Arthropoda Ameiridae Nitokra -

II Evaluate water quality conditions to determine applicability

bull Criteria are often based on one or more water quality conditions (eg hardness) that affect toxicity of the material to test organisms

bull Aluminum toxicity is highly dependent on the pH of the test waterndash The pH of test waters affects the amount and form

of aluminum that is bioavailable to test speciesndash Hardness and DOC (Dissolved Organic Carbon)

also influence the amount of aluminum that is bioavailable

16

Multiple Bioavailability Modeling Approaches Underwent External Peer Review

17

bull Biotic Ligand Modelsbull Full aluminum BLM (2016 model version) ndash Temperature pH

Dissolved organic carbon (DOC) Major cations (calcium [Ca] magnesium [Mg] sodium [Na] and potassium [K]) Major anions (sulfate [SO4] and chloride [Cl]) Alkalinity and Sulfide

bull Simplified aluminum BLM - pH hardness DOC temperature

bull Multilinear Regression Equationsbull pH and Hardness

Accounting for Effects of Water Chemistry on Bioavailability and Toxicity

bull Water quality criteria can be modified quantitatively when enough data are available to demonstrate that those conditions similarly affect the results with a variety of speciesndash High quality data in the draft aluminum document demonstrated

that pH DOC and hardness affected a variety of species in a similar way when exposed to aluminum concentrations

bull Recent publications by Cardwell et al (2017) and Gensemer et al (2017) summarized short-term aluminum chronic toxicity data across a range of DOC pH and hardness values for three species

18

Multiple Linear Regression (MLR) Models to Account for Effects of Water Chemistry

bull MLR models were developed for each species (DeForest et al 2017)ndash 119862119862 119889119889119906119906119887119887119894119894119886119886 11986411986411986211986220=119890119890[minus41026+[0525timesln(119863119863119874119874119862119862)]+[2201timesln(ℎ119886119886119903119903119889119889)]+(11282times119901119901119867119867)minus(0633times1199011199011198671198672)minus[0264times119901119901119867119867ln(ℎ119886119886119903119903119889119889)]]

ndash 119875119875 119901119901119903119903119900119900119898119898119890119890119897119897119886119886119904119904 11986411986411986211986220=119890119890[minus14029+[0503timesln(119863119863119874119874119862119862)]+[3443timesln(ℎ119886119886119903119903119889119889)]+(3131times119901119901119867119867)minus[0494times119901119901119867119867ln(ℎ119886119886119903119903119889119889)]]

bull These models were applied to both the acute and chronic aluminum toxicity datandash The C dubia model was applied to invertebrate datandash The P promelas model was applied to vertebrate data

19

III Normalize toxicity data in species sensitivity distribution to a set of water

quality conditionsbull Just like single parameter hardness models the MLR

models were used to normalize the aluminum toxicity data to a single set of water quality conditionsndash As an illustration for one set of calculations the draft

aluminum AWQC set these conditions to pH 7 hardness of 100 mgL and DOC of 1 mgL

bull This allows for comparisons to be made between species based on their sensitivity to aluminumndash Apples to apples

20

IV Construct sensitivity distribution to determine criteria at set conditions

21

Figure 8 Ranked Summary of Total Aluminum Genus Mean Acute Values (GMAVs) - Freshwater at pH 7 Hardness of 100 mgL and DOC of 1 mgL

IV Construct sensitivity distribution to determine criteria at set conditions

22

Figure 10 Ranked Summary of Total Aluminum Genus Mean Chronic Values (GMCVs) ndash Freshwater Supplemented with Other Data to Fulfill Missing MDRs at pH 7 Hardness of 100 mgL and DOC of 1 mgL

Aluminum Criteria Calculatorbull The relative ranking of genera in the sensitivity

distribution to which the toxicity data are normalized will change based on the set of water chemistry conditions (ie pH hardness and DOC)

bull To make calculating aluminum criteria easier EPA developed an Aluminum Criteria Calculatorndash Automated so that user only needs to enter water chemistry

conditions of interestndash Allows quick calculations for a variety of conditionsndash Limits calculations to the recommended conditions for which

the MLR models were developed were specified for hardness and DOC

bull No extrapolation beyond hardness and DOC empirical data bounds

23

Aluminum Criteria Calculator SD Example

24

pH=7 Hardness=100 DOC=1 pH=6 Hardness=25 DOC=1

Chronic Ranked GMCVRank GMCV Genus

12 17098 Aeolosoma Invert11 gt 9746 Rana Amphib - Other Data10 5908 Chironomus Invert

9 4877 Lymnaea Mollusk8 2555 Brachionus Invert7 2488 Pimephales Fish6 1713 Hyalella Invert5 1182 Ceriodaphnia Invert4 1102 Danio Fish3 1042 Lampsilis Mollusk2 591 Salvelinus Fish1 509 Salmo Fish

FCV = 3940 microgL total aluminum

Chronic Ranked GMCVRank GMCV Genus

12 gt 2131 Rana Amphib - Other Data11 1710 Aeolosoma Invert10 591 Chironomus Invert

9 544 Pimephales Fish8 488 Lymnaea Mollusk7 255 Brachionus Invert6 241 Danio Fish5 171 Hyalella Invert4 129 Salvelinus Fish3 118 Ceriodaphnia Invert2 111 Salmo Fish1 104 Lampsilis Mollusk

FCV = 987 microgL total aluminum

Multiple Scenarios

Run Scenarios

Summary Sheet

Acute Dataset

Chronic Dataset

Multiple Scenarios

Run Scenarios

Summary Sheet

Acute Dataset

Chronic Dataset

Boundaries of the Criteria Outputsbull MLR models were developed using data that

encompassndash DOC range of 008-5 mgLndash Hardness range of 98-127 mgL (as CaCO3)ndash pH range of 6-81

bull MLR criteria outputs are bounded atndash Maximum of 150 mgL total hardness (as CaCO3)

ndash DOC of 50 mgLndash pH range of the model is 50 to 90

25

Aluminum Criteria Look-Up Tables

26

bull EPA also provided criteria look-up tables in Appendix K

Har

dnes

s

CCC(microgL total aluminum)

(DOC=05 mgL)

pH 50 pH 55 pH 60 pH 65 pH 70 pH 75 pH 80 pH 85 pH 90

25 23 a 14 b 69 c 160 f 350 f 620 i 540 b 410 a 230 a

50 43 a 24 b 100 d 170 f 310 f 630 f 670 j 400 a 200 a

75 61 a 33 b 130 e 180 f 290 f 530 f 730 i 390 a 190 a

100 78 a 41 b 150 e 190 f 280 g 460 f 770 c 390 a 180 a

150 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

200 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

250 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

300 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

350 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

400 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

Aluminum Criteria Look-Up Tables

27

bull EPA also provided criteria look-up tables in Appendix K

Har

dnes

s

CCC(microgL total aluminum)

(DOC=10 mgL)

pH 50 pH 55 pH 60 pH 65 pH 70 pH 75 pH 80 pH 85 pH 90

25 33 a 21 b 99 c 220 f 490 f 900 i 780 b 590 a 330 a

50 61 a 35 b 150 e 250 f 440 f 890 f 960 j 580 a 290 a

75 88 a 47 b 180 e 260 f 410 f 740 f 1100 i 570 a 270 a

100 11 a 59 b 210 e 270 f 390 g 650 f 1100 c 560 b 260 a

150 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

200 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

250 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

300 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

350 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

400 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

Aluminum Criteria Look-Up Tables

28

bull EPA also provided criteria look-up tables in Appendix K

Har

dnes

s

CCC(microgL total aluminum)

(DOC=25 mgL)

pH 50 pH 55 pH 60 pH 65 pH 70 pH 75 pH 80 pH 85 pH 90

25 54 a 34 b 160 c 350 f 770 f 1500 i 1300 b 960 a 540 a

50 99 a 57 b 230 e 390 f 690 f 1400 f 1600 j 930 a 470 a

75 14 a 77 b 290 e 410 f 640 f 1200 f 1700 i 920 a 440 a

100 18 a 96 b 330 e 420 f 620 g 1000 f 1800 d 920 b 420 a

150 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

200 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

250 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

300 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

350 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

400 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

Aluminum Criteria Look-Up Tables

29

bull EPA also provided criteria look-up tables in Appendix K

Har

dnes

s

CCC(microgL total aluminum)

(DOC=50 mgL)

pH 50 pH 55 pH 60 pH 65 pH 70 pH 75 pH 80 pH 85 pH 90

25 77 a 49 b 230 c 500 f 1100 f 2100 c 1900 b 1400 a 770 a

50 14 a 82 b 330 e 540 f 970 f 2000 f 2200 j 1300 a 680 a

75 20 a 110 b 410 e 570 f 900 f 1600 f 2500 i 1300 a 640 a

100 26 a 140 b 460 f 590 f 880 g 1400 f 2500 d 1300 b 600 a

150 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

200 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

250 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

300 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

350 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

400 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

Status

30

bull Draft Document Released to the Federal Register on 72817ndash Public comment period of 60 days ndash Due Date for Comments is 92617

bull Revisions will be made considering public commentsbull Subsequent EPA intra-agency review of final criteria

document will then be conducted bull Publication of final criteria document planned for Late Fall

2017

Federal Register Webpagehttpswwwfederalregistergovdocuments201

707282017-15968request-for-scientific-views-draft-updated-aquatic-life-ambient-water-quality-criteria-for-aluminum

Aluminum Criteria Webpagehttpswwwepagovwqc2017-draft-aquatic-life-

criteria-aluminum-freshwater-documents

31

	Appendix C Acceptable Chronic Toxicity Data of Aluminum to Freshwater Aquatic Animals
									MLR equation
	Species		Hardness (mgL as CaCO3)		pH		DOC(mgL)		EC20 Endpoint			EC20(microgL)			Normalized Conc- EC20(microgL)			SMCV-EC20(microgL)		GMCV-EC20(microgL)			Genus			DOC Notes		Dilution water
	Oligochaete Aeolosoma sp		48		6		025		Reproduction (population size)			1235			1710			1710		1710			Aeolosoma		Invert		Author reported half the detection limit
	Rotifer Brachionus calyciflorus		100		62		025		Reproduction (population size)			431			255			255		255			Brachionus		Invert		Author reported half the detection limit
	Great pond snail Lymnaea stagnalis		117		61		025		Biomass			746			488			488		488			Lymnaea		Mollusk		Author reported half the detection limit
	Fatmucket Lampsilis siliquoidea		1055		604		040		Biomass			169			104			104		104			Lampsilis		Mollusk		Author reported
	Cladoceran Ceriodaphnia dubia		50		715		11		Reproduction - youngstarting adult			1780			190						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		26		73		05		Reproduction - youngfemale			1557			272						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		46		75		05		Reproduction - youngfemale			8087			110						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		96		79		05		Reproduction - youngfemale			6519			80						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		194		81		05		Reproduction - youngfemale			6836			89						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		255		703		05		Reproduction (youngfemale)			250			56						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		122		713		05		Reproduction (youngfemale)			860			106						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		25		798		05		Reproduction (youngfemale)			700			101						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		61		803		05		Reproduction (youngfemale)			1010			136						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		122		810		05		Reproduction (youngfemale)			870			116						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		255		633		05		Reproduction (youngfemale)			260			176						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		121		636		05		Reproduction (youngfemale)			390			110			118		118			Ceriodaphnia		Invert		Author reported
	Amphipod Hyalella azteca		95		61		051		Biomass (28 d)			1993			101						Hyalella			Author reported
	Amphipod Hyalella azteca		106		604		033		Biomass (28 d)			425			289			171		171			Hyalella		Invert		Author reported
	Midge Chironomus riparius		118		558		18		Adult midge emergence			2955			119						Chironomus			Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Midge Chironomus riparius		119		505		18		Adult midge emergence			8442			2258						Chironomus			Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Midge Chironomus riparius		91		66		051		Reproduction ( of eggscase)			3387			766			591		591			Chironomus		Invert		Author reported
	Atlantic salmon Salmo salar		127		57		18		Biomass			6156			1112			1112		1112			Salmo		Fish		Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Brook trout Salvelinus fontinalis		123		655		19		Biomass			1644			59						Salvelinus			Author reported		wellRO mix
	Brook trout Salvelinus fontinalis		128		565		18		Biomass			1435			283			1293		1293			Salvelinus		Fish		Author reported		wellRO mix
	Fathead minnow Pimephales promelas		220		770		16		Biomass			6194			781						Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		well water
	Fathead minnow Pimephales promelas		96		620		025		Fry survival			4286			379			544		544			Pimephales		Fish		Author reported half the detection limit
	Zebrafish Danio rerio		83		61		025		Biomass			2344			241			241		241			Danio		Fish		Author reported half the detection limit
	Wood frog Rana sylvatica		115		469		16		MATC-survival		gt		2000		gt		2131		gt		2131		2131		gt		Rana		Amphib - Other Data		Value is from 2007 FW Cu AWQC Appendix C recommendation		aged tap water

	Appendix A Acceptable Acute Toxicity Data of Aluminum to Freshwater Aquatic Animals
	Species		Methoda		Hardness (mgL as CaCO3)		pH		DOC(mgL)			LC50 or EC50 Conc (microgL)		Reference			Normalized Conc - LC50(microgL)			Species Mean Acute Value(microgL)		Genus Mean Acute Value(microgL)			Genus			DOC Note		Dilution water
	Snail Physa sp		S M T		474		659		11		gt		23400		Call 1984 Call et al 1984		gt		4843			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		755		11			30600		Call 1984 Call et al 1984			2699			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		817		11		gt		24700		Call 1984 Call et al 1984		gt		2404			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		746		11			55500		Call 1984 Call et al 1984			5034			3546		3546			Physa		Mollusk		Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Fatmucket Lampsilis siliquoidea		F M T		1060		612		048		gt		6302		Wang et al 2016 Wang et al 2017		gt		2983		gt		2983		2983		gt		Lampsilis		Mollusk		Author reported
	Cladoceran Ceriodaphnia dubia		S M A		500		742		11			1900		McCauley et al 1986			173			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		S M A		505		786		11			1500		McCauley et al 1986			129			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		UW-S Lake Superior lab water
	Cladoceran Ceriodaphnia dubia		S M A		500		813		11			2560		McCauley et al 1986			243			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		R M T		26		75		05			720		ENSR 1992d			111			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		R M T		46		76		05			1880		ENSR 1992d			250			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		R M T		96		78		05			2450		ENSR 1992d			289			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		S U NR		90		715		05			3727		Fort and Stover 1995			500			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U NR		90		715		05			5673		Fort and Stover 1995			761			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U NR		89		76		05			2880		Soucek et al 2001			336			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		601		05			7112		European Al Association 2009			169			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		605		2			6865		European Al Association 2009			709			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		609		4			15581		European Al Association 2009			1007			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		601		05			681		European Al Association 2009			162			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		603		05			1630		European Al Association 2009			367			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		597		05			1785		European Al Association 2009			473			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		592		05			1410		European Al Association 2009			429			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		699		05		gt		1300		European Al Association 2009		gt		419			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		785		05		gt		5000		European Al Association 2009		gt		796			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		68		2		gt		10000		European Al Association 2009		gt		2076			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		782		2		gt		15000		European Al Association 2009		gt		1163			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		677		4		gt		10000		European Al Association 2009		gt		1516			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		766		4		gt		15000		European Al Association 2009		gt		861			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		79		05		gt		2000		European Al Association 2009		gt		315			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		789		05		gt		2000		European Al Association 2009		gt		316			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		604		05			1108		European Al Association 2009			85			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		598		2			11371		European Al Association 2009			482			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		573		4			80467		European Al Association 2009			4357			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		671		05		gt		10000		European Al Association 2009		gt		2379			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		783		05		gt		5000		European Al Association 2009		gt		634			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		679		2		gt		10000		European Al Association 2009		gt		1039			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		767		2		gt		15000		European Al Association 2009		gt		908			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		668		4		gt		15000		European Al Association 2009		gt		1246			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		762		4		gt		15000		European Al Association 2009		gt		633			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		606		2			33868		European Al Association 2009			792			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		56		4			104842		European Al Association 2009			4880			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		693		05		gt		5000		European Al Association 2009		gt		713			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		120		788		05		gt		5000		European Al Association 2009		gt		590			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		120		676		2		gt		15000		European Al Association 2009		gt		1212			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		771		2		gt		15000		European Al Association 2009		gt		810			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		66		4		gt		15000		European Al Association 2009		gt		1014			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		76		4		gt		15000		European Al Association 2009		gt		555			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			11971		European Al Association 2010			270			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			27478		European Al Association 2010			619			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			11998		European Al Association 2010			270			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		607		05			92495		European Al Association 2010			188			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		609		05			31337		European Al Association 2010			604			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		61		05			33235		European Al Association 2010			624			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		708		05		gt		8864		European Al Association 2010		gt		253			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		779		05		gt		42783		European Al Association 2010		gt		693			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		753		05			13204		European Al Association 2010			24			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		600		601		05			46326		European Al Association 2010			380			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		600		599		05		gt		8590		European Al Association 2010		gt		737			509				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia reticulata		F M T		451		60		11			304		Shephard 1983			201			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia reticulata		F M T		40		55		11			362		Shephard 1983			5531			1054		733			Ceriodaphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake SuperiorDI Mix
	Cladoceran Daphnia magna		S U NR		453		774		11			3900		Biesinger and Christensen 1972			338			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Daphnia magna		S M T		220		76		16			38200		Kimball 1978			2030			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Well water
	Cladoceran Daphnia magna		S U T		451		725		11			2800		Shephard 1983			287			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Daphnia magna		S U T		168		599		05			5000		European Al Association 2009		gt		227			-				Daphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Daphnia magna		S U T		168		792		05			7950		European Al Association 2009			92			333		333			Daphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Ostracod Stenocypris major		R M T		1563		651		16			310196		Shuhaimi-Othman et al 2011a			1022			1022		1022			Stenocypris		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (Malaysia)
	Amphipod Crangonyx pseudogracilis		R U T		50		675		16			9190		Martin and Holdich 1986			1217			1217		1217			Crangonyx		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (UK)
	Amphipod Hyalella azteca		F M T		105		613		048		gt		5997		Wang et al 2016 Wang et al 2017		gt		2800		gt		2800		2800		gt		Hyalella		Invert		Author reported
	Stonefly Acroneuria sp		S M T		474		746		11		gt		22600		Call et al 1984		gt		2050		gt		2050		2050		gt		Acroneuria		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Midge Chironomus plumosus		S U T		80		70		16			30000		Fargasova 2001			2536			2536		2536			Chironomus		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (Slovakia)
	Midge Paratanytarsus dissimilis		S M T		1743		728		28		gt		77700		Lamb and Bailey 1981 1983		gt		6232		gt		6232		6232		gt		Paratanytarsus		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Liberty Lake Washington
	Rainbow trout Oncorhynchus mykiss		F M T		274		758		05		gt		9840		Gundersen et al 1994		gt		1256			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		452		762		05		gt		8070		Gundersen et al 1994		gt		1140			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		895		758		05		gt		8160		Gundersen et al 1994		gt		1489			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		1304		758		05		gt		8200		Gundersen et al 1994		gt		1676			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		232		825		05			6170		Gundersen et al 1994			260			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		350		826		05			6170		Gundersen et al 1994			333			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		836		829		05			7670		Gundersen et al 1994			701			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		1158		830		05			6930		Gundersen et al 1994			777			801		801			Oncorhynchus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Atlantic salmon Salmo salar		S U T		68		55		05			584		Hamilton and Haines 1995			4602			-				Salmo			Value is from 2007 FW Cu AWQC Appendix C recommendation		Soft recon
	Atlantic salmon Salmo salar		S U T		68		65		05			599		Hamilton and Haines 1995			531			1564		1564			Salmo		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Soft recon
	Brook trout Salvelinus fontinalis		S U T		40		56		16			6530		Tandjung 1982			6947			-				Salvelinus			Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Brook trout Salvelinus fontinalis		S U T		18		56		16			3400		Tandjung 1982			6209			-				Salvelinus			Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Brook trout Salvelinus fontinalis		S U T		2		56		16			370		Tandjung 1982			2988			5051		5051			Salvelinus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Green sunfish Lepomis cyanellus		S M T		474		755		11		gt		50000		Call et al 1984		gt		5255		gt		5255		5255		gt		Lepomis		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Rio Grande silvery minnow Hybognathus amarus		R M T		140		81		05		gt		59100		Buhl 2002		gt		8620		gt		8620		8620		gt		Hybognathus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon water
	Fathead minnow Pimephales promelas		S M T		474		761		11		gt		48200		Call et al 1984		gt		4707			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Fathead minnow Pimephales promelas		S M T		474		805		11		gt		49800		Call et al 1984		gt		2837			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Fathead minnow Pimephales promelas		R M T		26		78		05			1160		ENSR 1992c			104			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		46		76		05			8180		ENSR 1992c			1191			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		96		81		05			20300		ENSR 1992c			2398			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		194		81		05			44800		ENSR 1992c			7839			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		140		81		05		gt		59100		Buhl 2002		gt		8620			2224		2224			Pimephales		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon water
	Smallmouth bass Micropterus dolomieui		S M T		1245		505		16			130		Kane and Rabeni 1987			859			859		859			Micropterus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Green tree frog Hyla cinerea		R M T		455		549		05		gt		4052		Jung and Jagoe 1995		gt		4377		gt		4377		4377		gt		Hyla		Amphibian		Value is from 2007 FW Cu AWQC Appendix C recommendation		artificial soft recon

	Normalization Chemistry
	pH		6					FAV		360
	Hardness		25					CMC		180
	DOC		1					CCC		99
	Acute Ranked GMAV											Chronic Ranked GMCV
	Rank			GMAV		Genus								Rank			GMCV		Genus
	18		gt		8620		Hybognathus		Fish							12		gt		2131		Rana		Amphib - Other Data
	17		gt		6232		Paratanytarsus		Invert							11			1710		Aeolosoma		Invert
	16		gt		5255		Lepomis		Fish							10			591		Chironomus		Invert
	15			5051		Salvelinus		Fish							9			544		Pimephales		Fish
	14		gt		4377		Hyla		Amphibian							8			488		Lymnaea		Mollusk
	13			3546		Physa		Mollusk							7			255		Brachionus		Invert
	12		gt		2983		Lampsilis		Mollusk							6			241		Danio		Fish
	11		gt		2800		Hyalella		Invert							5			171		Hyalella		Invert
	10			2536		Chironomus		Invert							4			129		Salvelinus		Fish
	9			2224		Pimephales		Fish							3			118		Ceriodaphnia		Invert
	8		gt		2050		Acroneuria		Invert							2			111		Salmo		Fish
	7			1564		Salmo		Fish							1			104		Lampsilis		Mollusk
	6			1217		Crangonyx		Invert
	5			1022		Stenocypris		Invert
	4			859		Micropterus		Fish
	3			801		Oncorhynchus		Fish
	2			733		Ceriodaphnia		Invert
	1			333		Daphnia		Invert
		N		Rank		Genus		GMAV		ln(GMAV)		ln(GMAV)2		P=R(N+1)		sqrt(P)				N		Rank		Genus		GMCV		ln(GMCV)		ln(GMCV)2		P=R(N+1)		sqrt(P)
		18		4		Micropterus		859		676		4564		0211		0459				12		4		Salvelinus		129		486		2364		0308		0555
			3		Oncorhynchus		801		669		4469		0158		0397					3		Ceriodaphnia		118		477		2277		0231		0480
			2		Ceriodaphnia		733		660		4352		0105		0324					2		Salmo		111		471		2220		0154		0392
			1		Daphnia		333		581		3374		0053		0229					1		Lampsilis		104		465		2159		0077		0277
			Sum				2585		1676		0526		141					Sum				1899		902		0769		170
			S2 =		1987										S2 =		059
			L =		4890										L =		4420
			A =		5887										A =		4592
			FAV =		360										FCV =		987
			CMC =		180

				All concentrations reported are microgL total Aluminum
				lt-----Click Button after entering data
	ENTER DATA HERE									ERRORNUM		ACUTE														CHRONIC
										Rank 1				Rank 2				Rank 3				Rank 4					Rank 1				Rank 2				Rank 3				Rank 4
	SiteName		DOC(mgL)		Hardness(mgL as CaCO3)		pH			FAV		CMC		CCC			Genus		GMAV			Genus		GMAV			Genus		GMAV			Genus		GMAV				Genus		GMCV			Genus		GMCV			Genus		GMCV			Genus		GMCV
	Site 1		50		25		9		75428737034162		35229324666008		1800		770			Daphnia			33452		Ceriodaphnia			73576		Stenocypris			102576		Crangonyx			122230			Lampsilis			10463		Ceriodaphnia			11865		Hyalella			17200		Brachionus			25648
	Site 2		50		50		9		75428737034162		31204991509063		1600		680			Daphnia			29631		Ceriodaphnia			65171		Stenocypris			90859		Crangonyx			108267			Lampsilis			9268		Ceriodaphnia			10510		Hyalella			15236		Brachionus			22718
	Site 3		50		75		9		75428737034162		29067527928199		1500		640			Daphnia			27601		Ceriodaphnia			60707		Stenocypris			84635		Crangonyx			100851			Lampsilis			8633		Ceriodaphnia			9790		Hyalella			14192		Brachionus			21162
	Site 4		50		100		9		75428737034162		27640367912592		1400		600			Daphnia			26246		Ceriodaphnia			57726		Stenocypris			80480		Crangonyx			95899			Lampsilis			8209		Ceriodaphnia			9309		Hyalella			13495		Brachionus			20123
	Site 5		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 6		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 7		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 8		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 9		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 10		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 11		50		25		85		75428737034162		63245275664384		3200		1400			Daphnia			60055		Ceriodaphnia			132086		Stenocypris			184149		Crangonyx			219432			Lampsilis			18783		Ceriodaphnia			21301		Hyalella			30879		Brachionus			46045
	Site 12		50		50		85		75428737034162		61388044609926		3100		1300			Daphnia			58291		Ceriodaphnia			128208		Stenocypris			178742		Crangonyx			212988			Lampsilis			18232		Ceriodaphnia			20676		Hyalella			29972		Brachionus			44693
	Site 13		50		75		85		75428737034162		60327020429132		3000		1300			Daphnia			57284		Ceriodaphnia			125992		Stenocypris			175652		Crangonyx			209307			Lampsilis			17917		Ceriodaphnia			20318		Hyalella			29454		Brachionus			43920
	Site 14		50		100		85		75428737034162		59585352130146		3000		1300			Daphnia			56580		Ceriodaphnia			124443		Stenocypris			173493		Crangonyx			206734			Lampsilis			17696		Ceriodaphnia			20068		Hyalella			29092		Salmo			41264
	Site 15		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 16		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 17		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 18		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 19		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 20		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370

	Appendix C Acceptable Chronic Toxicity Data of Aluminum to Freshwater Aquatic Animals
									MLR equation
	Species		Hardness (mgL as CaCO3)		pH		DOC(mgL)		EC20 Endpoint			EC20(microgL)			Normalized Conc- EC20(microgL)			SMCV-EC20(microgL)		GMCV-EC20(microgL)			Genus			DOC Notes		Dilution water
	Oligochaete Aeolosoma sp		48		6		025		Reproduction (population size)			1235			17098			17098		17098			Aeolosoma		Invert		Author reported half the detection limit
	Rotifer Brachionus calyciflorus		100		62		025		Reproduction (population size)			431			2555			2555		2555			Brachionus		Invert		Author reported half the detection limit
	Great pond snail Lymnaea stagnalis		117		61		025		Biomass			746			4877			4877		4877			Lymnaea		Mollusk		Author reported half the detection limit
	Fatmucket Lampsilis siliquoidea		1055		604		040		Biomass			169			1042			1042		1042			Lampsilis		Mollusk		Author reported
	Cladoceran Ceriodaphnia dubia		50		715		11		Reproduction - youngstarting adult			1780			1898						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		26		73		05		Reproduction - youngfemale			1557			2718						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		46		75		05		Reproduction - youngfemale			8087			1102						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		96		79		05		Reproduction - youngfemale			6519			796						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		194		81		05		Reproduction - youngfemale			6836			888						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		255		703		05		Reproduction (youngfemale)			250			563						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		122		713		05		Reproduction (youngfemale)			860			1061						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		25		798		05		Reproduction (youngfemale)			700			1006						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		61		803		05		Reproduction (youngfemale)			1010			1363						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		122		810		05		Reproduction (youngfemale)			870			1164						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		255		633		05		Reproduction (youngfemale)			260			1758						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		121		636		05		Reproduction (youngfemale)			390			1101			1182		1182			Ceriodaphnia		Invert		Author reported
	Amphipod Hyalella azteca		95		61		051		Biomass (28 d)			1993			1014						Hyalella			Author reported
	Amphipod Hyalella azteca		106		604		033		Biomass (28 d)			425			2895			1713		1713			Hyalella		Invert		Author reported
	Midge Chironomus riparius		118		558		18		Adult midge emergence			2955			1192						Chironomus			Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Midge Chironomus riparius		119		505		18		Adult midge emergence			8442			22578						Chironomus			Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Midge Chironomus riparius		91		66		051		Reproduction ( of eggscase)			3387			7664			5908		5908			Chironomus		Invert		Author reported
	Atlantic salmon Salmo salar		127		57		18		Biomass			6156			5085			5085		5085			Salmo		Fish		Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Brook trout Salvelinus fontinalis		123		655		19		Biomass			1644			270						Salvelinus			Author reported		wellRO mix
	Brook trout Salvelinus fontinalis		128		565		18		Biomass			1435			1295			5914		5914			Salvelinus		Fish		Author reported		wellRO mix
	Fathead minnow Pimephales promelas		220		770		16		Biomass			6194			3569						Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		well water
	Fathead minnow Pimephales promelas		96		620		025		Fry survival			4286			1734			2488		2488			Pimephales		Fish		Author reported half the detection limit
	Zebrafish Danio rerio		83		61		025		Biomass			2344			1102			1102		1102			Danio		Fish		Author reported half the detection limit
	Wood frog Rana sylvatica		115		469		16		MATC-survival		gt		2000		gt		9746		gt		9746		9746		gt		Rana		Amphib - Other Data		Value is from 2007 FW Cu AWQC Appendix C recommendation		aged tap water

	Appendix A Acceptable Acute Toxicity Data of Aluminum to Freshwater Aquatic Animals
	Species		Methoda		Hardness (mgL as CaCO3)		pH		DOC(mgL)			LC50 or EC50 Conc (microgL)		Reference			Normalized Conc - LC50(microgL)			Species Mean Acute Value(microgL)		Genus Mean Acute Value(microgL)			Genus			DOC Note		Dilution water
	Snail Physa sp		S M T		474		659		11		gt		23400		Call 1984 Call et al 1984		gt		48425			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		755		11			30600		Call 1984 Call et al 1984			26986			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		817		11		gt		24700		Call 1984 Call et al 1984		gt		24040			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		746		11			55500		Call 1984 Call et al 1984			50338			35462		35462			Physa		Mollusk		Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Fatmucket Lampsilis siliquoidea		F M T		1060		612		048		gt		6302		Wang et al 2016 Wang et al 2017		gt		29834		gt		29834		29834		gt		Lampsilis		Mollusk		Author reported
	Cladoceran Ceriodaphnia dubia		S M A		500		742		11			1900		McCauley et al 1986			1728			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		S M A		505		786		11			1500		McCauley et al 1986			1294			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		UW-S Lake Superior lab water
	Cladoceran Ceriodaphnia dubia		S M A		500		813		11			2560		McCauley et al 1986			2431			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		R M T		26		75		05			720		ENSR 1992d			1113			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		R M T		46		76		05			1880		ENSR 1992d			2495			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		R M T		96		78		05			2450		ENSR 1992d			2894			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		S U NR		90		715		05			3727		Fort and Stover 1995			5002			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U NR		90		715		05			5673		Fort and Stover 1995			7613			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U NR		89		76		05			2880		Soucek et al 2001			3362			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		601		05			7112		European Al Association 2009			1691			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		605		2			6865		European Al Association 2009			7088			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		609		4			15581		European Al Association 2009			10072			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		601		05			681		European Al Association 2009			1620			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		603		05			1630		European Al Association 2009			3674			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		597		05			1785		European Al Association 2009			4732			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		592		05			1410		European Al Association 2009			4295			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		699		05		gt		1300		European Al Association 2009		gt		4189			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		785		05		gt		5000		European Al Association 2009		gt		7960			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		68		2		gt		10000		European Al Association 2009		gt		20755			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		782		2		gt		15000		European Al Association 2009		gt		11626			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		677		4		gt		10000		European Al Association 2009		gt		15161			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		766		4		gt		15000		European Al Association 2009		gt		8608			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		79		05		gt		2000		European Al Association 2009		gt		3150			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		789		05		gt		2000		European Al Association 2009		gt		3156			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		604		05			1108		European Al Association 2009			850			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		598		2			11371		European Al Association 2009			4817			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		573		4			80467		European Al Association 2009			43570			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		671		05		gt		10000		European Al Association 2009		gt		23786			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		783		05		gt		5000		European Al Association 2009		gt		6344			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		679		2		gt		10000		European Al Association 2009		gt		10394			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		767		2		gt		15000		European Al Association 2009		gt		9082			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		668		4		gt		15000		European Al Association 2009		gt		12461			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		762		4		gt		15000		European Al Association 2009		gt		6332			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		606		2			33868		European Al Association 2009			7920			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		56		4			104842		European Al Association 2009			48800			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		693		05		gt		5000		European Al Association 2009		gt		7126			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		120		788		05		gt		5000		European Al Association 2009		gt		5898			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		120		676		2		gt		15000		European Al Association 2009		gt		12117			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		771		2		gt		15000		European Al Association 2009		gt		8096			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		66		4		gt		15000		European Al Association 2009		gt		10139			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		76		4		gt		15000		European Al Association 2009		gt		5545			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			11971		European Al Association 2010			2699			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			27478		European Al Association 2010			6194			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			11998		European Al Association 2010			2705			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		607		05			92495		European Al Association 2010			1876			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		609		05			31337		European Al Association 2010			6035			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		61		05			33235		European Al Association 2010			6238			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		708		05		gt		8864		European Al Association 2010		gt		2534			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		779		05		gt		42783		European Al Association 2010		gt		6930			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		753		05			13204		European Al Association 2010			244			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		600		601		05			46326		European Al Association 2010			3798			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		600		599		05		gt		8590		European Al Association 2010		gt		7366			5094				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia reticulata		F M T		451		60		11			304		Shephard 1983			2009			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia reticulata		F M T		40		55		11			362		Shephard 1983			55307			10542		7328			Ceriodaphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake SuperiorDI Mix
	Cladoceran Daphnia magna		S U NR		453		774		11			3900		Biesinger and Christensen 1972			3382			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Daphnia magna		S M T		220		76		16			38200		Kimball 1978			20303			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Well water
	Cladoceran Daphnia magna		S U T		451		725		11			2800		Shephard 1983			2868			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Daphnia magna		S U T		168		599		05			5000		European Al Association 2009		gt		2265			-				Daphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Daphnia magna		S U T		168		792		05			7950		European Al Association 2009			920			3332		3332			Daphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Ostracod Stenocypris major		R M T		1563		651		16			310196		Shuhaimi-Othman et al 2011a			10216			10216		10216			Stenocypris		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (Malaysia)
	Amphipod Crangonyx pseudogracilis		R U T		50		675		16			9190		Martin and Holdich 1986			12174			12174		12174			Crangonyx		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (UK)
	Amphipod Hyalella azteca		F M T		105		613		048		gt		5997		Wang et al 2016 Wang et al 2017		gt		28002		gt		28002		28002		gt		Hyalella		Invert		Author reported
	Stonefly Acroneuria sp		S M T		474		746		11		gt		22600		Call et al 1984		gt		20498		gt		20498		20498		gt		Acroneuria		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Midge Chironomus plumosus		S U T		80		70		16			30000		Fargasova 2001			25361			25361		25361			Chironomus		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (Slovakia)
	Midge Paratanytarsus dissimilis		S M T		1743		728		28		gt		77700		Lamb and Bailey 1981 1983		gt		62318		gt		62318		62318		gt		Paratanytarsus		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Liberty Lake Washington
	Rainbow trout Oncorhynchus mykiss		F M T		274		758		05		gt		9840		Gundersen et al 1994		gt		5745			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		452		762		05		gt		8070		Gundersen et al 1994		gt		5212			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		895		758		05		gt		8160		Gundersen et al 1994		gt		6807			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		1304		758		05		gt		8200		Gundersen et al 1994		gt		7662			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		232		825		05			6170		Gundersen et al 1994			1190			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		350		826		05			6170		Gundersen et al 1994			1523			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		836		829		05			7670		Gundersen et al 1994			3205			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		1158		830		05			6930		Gundersen et al 1994			3554			3661		3661			Oncorhynchus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Atlantic salmon Salmo salar		S U T		68		55		05			584		Hamilton and Haines 1995			21042			-				Salmo			Value is from 2007 FW Cu AWQC Appendix C recommendation		Soft recon
	Atlantic salmon Salmo salar		S U T		68		65		05			599		Hamilton and Haines 1995			2430			7151		7151			Salmo		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Soft recon
	Brook trout Salvelinus fontinalis		S U T		40		56		16			6530		Tandjung 1982			31767			-				Salvelinus			Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Brook trout Salvelinus fontinalis		S U T		18		56		16			3400		Tandjung 1982			28391			-				Salvelinus			Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Brook trout Salvelinus fontinalis		S U T		2		56		16			370		Tandjung 1982			13663			23097		23097			Salvelinus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Green sunfish Lepomis cyanellus		S M T		474		755		11		gt		50000		Call et al 1984		gt		24028		gt		24028		24028		gt		Lepomis		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Rio Grande silvery minnow Hybognathus amarus		R M T		140		81		05		gt		59100		Buhl 2002		gt		39414		gt		39414		39414		gt		Hybognathus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon water
	Fathead minnow Pimephales promelas		S M T		474		761		11		gt		48200		Call et al 1984		gt		21522			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Fathead minnow Pimephales promelas		S M T		474		805		11		gt		49800		Call et al 1984		gt		12972			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Fathead minnow Pimephales promelas		R M T		26		78		05			1160		ENSR 1992c			477			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		46		76		05			8180		ENSR 1992c			5446			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		96		81		05			20300		ENSR 1992c			10966			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		194		81		05			44800		ENSR 1992c			35847			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		140		81		05		gt		59100		Buhl 2002		gt		39414			10168		10168			Pimephales		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon water
	Smallmouth bass Micropterus dolomieui		S M T		1245		505		16			130		Kane and Rabeni 1987			3929			3929		3929			Micropterus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Green tree frog Hyla cinerea		R M T		455		549		05		gt		4052		Jung and Jagoe 1995		gt		20016		gt		20016		20016		gt		Hyla		Amphibian		Value is from 2007 FW Cu AWQC Appendix C recommendation		artificial soft recon

	Normalization Chemistry
	pH		7					FAV		2741
	Hardness		100					CMC		1400
	DOC		1					CCC		390
	Acute Ranked GMAV											Chronic Ranked GMCV
	Rank			GMAV		Genus								Rank			GMCV		Genus
	18		gt		62318		Paratanytarsus		Invert							12			17098		Aeolosoma		Invert
	17		gt		39414		Hybognathus		Fish							11		gt		9746		Rana		Amphib - Other Data
	16			35462		Physa		Mollusk							10			5908		Chironomus		Invert
	15		gt		29834		Lampsilis		Mollusk							9			4877		Lymnaea		Mollusk
	14		gt		28002		Hyalella		Invert							8			2555		Brachionus		Invert
	13			25361		Chironomus		Invert							7			2488		Pimephales		Fish
	12		gt		24028		Lepomis		Fish							6			1713		Hyalella		Invert
	11			23097		Salvelinus		Fish							5			1182		Ceriodaphnia		Invert
	10		gt		20498		Acroneuria		Invert							4			1102		Danio		Fish
	9		gt		20016		Hyla		Amphibian							3			1042		Lampsilis		Mollusk
	8			12174		Crangonyx		Invert							2			591		Salvelinus		Fish
	7			10216		Stenocypris		Invert							1			509		Salmo		Fish
	6			10168		Pimephales		Fish
	5			7328		Ceriodaphnia		Invert
	4			7151		Salmo		Fish
	3			3929		Micropterus		Fish
	2			3661		Oncorhynchus		Fish
	1			3332		Daphnia		Invert
		N		Rank		Genus		GMAV		ln(GMAV)		ln(GMAV)2		P=R(N+1)		sqrt(P)				N		Rank		Genus		GMCV		ln(GMCV)		ln(GMCV)2		P=R(N+1)		sqrt(P)
		18		4		Salmo		7151		887		7876		0211		0459				12		4		Danio		1102		700		4907		0308		0555
			3		Micropterus		3929		828		6849		0158		0397					3		Lampsilis		1042		695		4829		0231		0480
			2		Oncorhynchus		3661		821		6733		0105		0324					2		Salvelinus		591		638		4074		0154		0392
			1		Daphnia		3332		811		6579		0053		0229					1		Salmo		509		623		3883		0077		0277
			Sum				3347		2804		0526		141					Sum				2657		1769		0769		170
			S2 =		1223										S2 =		1080
			L =		7134										L =		5242
			A =		7916										A =		5976
			FAV =		2741										FCV =		3940
			CMC =		1371

				All concentrations reported are microgL total Aluminum
				lt-----Click Button after entering data
	ENTER DATA HERE									ERRORNUM		ACUTE														CHRONIC
										Rank 1				Rank 2				Rank 3				Rank 4					Rank 1				Rank 2				Rank 3				Rank 4
	SiteName		DOC(mgL)		Hardness(mgL as CaCO3)		pH			FAV		CMC		CCC			Genus		GMAV			Genus		GMAV			Genus		GMAV			Genus		GMAV				Genus		GMCV			Genus		GMCV			Genus		GMCV			Genus		GMCV
	Site 1		50		25		9		75428737034162		35229324666008		1800		770			Daphnia			33452		Ceriodaphnia			73576		Stenocypris			102576		Crangonyx			122230			Lampsilis			10463		Ceriodaphnia			11865		Hyalella			17200		Brachionus			25648
	Site 2		50		50		9		75428737034162		31204991509063		1600		680			Daphnia			29631		Ceriodaphnia			65171		Stenocypris			90859		Crangonyx			108267			Lampsilis			9268		Ceriodaphnia			10510		Hyalella			15236		Brachionus			22718
	Site 3		50		75		9		75428737034162		29067527928199		1500		640			Daphnia			27601		Ceriodaphnia			60707		Stenocypris			84635		Crangonyx			100851			Lampsilis			8633		Ceriodaphnia			9790		Hyalella			14192		Brachionus			21162
	Site 4		50		100		9		75428737034162		27640367912592		1400		600			Daphnia			26246		Ceriodaphnia			57726		Stenocypris			80480		Crangonyx			95899			Lampsilis			8209		Ceriodaphnia			9309		Hyalella			13495		Brachionus			20123
	Site 5		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 6		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 7		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 8		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 9		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 10		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 11		50		25		85		75428737034162		63245275664384		3200		1400			Daphnia			60055		Ceriodaphnia			132086		Stenocypris			184149		Crangonyx			219432			Lampsilis			18783		Ceriodaphnia			21301		Hyalella			30879		Brachionus			46045
	Site 12		50		50		85		75428737034162		61388044609926		3100		1300			Daphnia			58291		Ceriodaphnia			128208		Stenocypris			178742		Crangonyx			212988			Lampsilis			18232		Ceriodaphnia			20676		Hyalella			29972		Brachionus			44693
	Site 13		50		75		85		75428737034162		60327020429132		3000		1300			Daphnia			57284		Ceriodaphnia			125992		Stenocypris			175652		Crangonyx			209307			Lampsilis			17917		Ceriodaphnia			20318		Hyalella			29454		Brachionus			43920
	Site 14		50		100		85		75428737034162		59585352130146		3000		1300			Daphnia			56580		Ceriodaphnia			124443		Stenocypris			173493		Crangonyx			206734			Lampsilis			17696		Ceriodaphnia			20068		Hyalella			29092		Salmo			41264
	Site 15		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 16		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 17		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 18		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 19		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 20		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370

Aluminum Toxicity Data

13

bull New data and models examinedndash Data submitted to EU REACH program

bull New freshwater aluminum toxicity data generated by Oregon State University

bull 2016 BLM model provided by the European Aluminum Association

ndash Saltwater data provided by Australia bull Insufficient data to develop estuarinemarine acute or chronic

criteria at this timendash Multiple Linear Regression (MLR) model developed by

DeForest et al 2017bull DeForest DK KV Brix LM Tear and WJ Adams 2017 Multiple Linear Regression (MLR) models for predicting chronic aluminum

toxicity to freshwater aquatic organisms and developing water quality guidelines Environ Toxicol Chem (DOI101002etc3922)

14

Minimum Dataset For Freshwater Criteria Derivation

SALMONID SECONDFISHFAMILY

CHORDATA

PLANKTONICCRUSTACEAN

BENTHIC CRUSTACEAN

INSECT ROTIFERA ANNELIDA MOLLUSCA

OTHERINSECT ORMOLLUSCA

EstuarineMarine Minimum Data Requirements

15

Family Minimum Data Requirement EstuarineMarine

Acute(Phylum Family Genus)

Chronic(Phylum Family Genus)

Family in the phylum Chordata - -

Family in the phylum Chordata - -

Either the Mysidae or Penaeidae family - -

Family in a phylum other than Arthropoda or Chordata Mollusca Ostreidae Crassostrea -

Family in a phylum other than Chordata Annelida Nereididae Neanthes -

Family in a phylum other than Chordata Annelida Capitellidae Capitella -

Family in a phylum other than Chordata Annelida Ctenodrilidae Ctenodrilus -

Any other family Arthropoda Ameiridae Nitokra -

II Evaluate water quality conditions to determine applicability

bull Criteria are often based on one or more water quality conditions (eg hardness) that affect toxicity of the material to test organisms

bull Aluminum toxicity is highly dependent on the pH of the test waterndash The pH of test waters affects the amount and form

of aluminum that is bioavailable to test speciesndash Hardness and DOC (Dissolved Organic Carbon)

also influence the amount of aluminum that is bioavailable

16

Multiple Bioavailability Modeling Approaches Underwent External Peer Review

17

bull Biotic Ligand Modelsbull Full aluminum BLM (2016 model version) ndash Temperature pH

Dissolved organic carbon (DOC) Major cations (calcium [Ca] magnesium [Mg] sodium [Na] and potassium [K]) Major anions (sulfate [SO4] and chloride [Cl]) Alkalinity and Sulfide

bull Simplified aluminum BLM - pH hardness DOC temperature

bull Multilinear Regression Equationsbull pH and Hardness

Accounting for Effects of Water Chemistry on Bioavailability and Toxicity

bull Water quality criteria can be modified quantitatively when enough data are available to demonstrate that those conditions similarly affect the results with a variety of speciesndash High quality data in the draft aluminum document demonstrated

that pH DOC and hardness affected a variety of species in a similar way when exposed to aluminum concentrations

bull Recent publications by Cardwell et al (2017) and Gensemer et al (2017) summarized short-term aluminum chronic toxicity data across a range of DOC pH and hardness values for three species

18

Multiple Linear Regression (MLR) Models to Account for Effects of Water Chemistry

bull MLR models were developed for each species (DeForest et al 2017)ndash 119862119862 119889119889119906119906119887119887119894119894119886119886 11986411986411986211986220=119890119890[minus41026+[0525timesln(119863119863119874119874119862119862)]+[2201timesln(ℎ119886119886119903119903119889119889)]+(11282times119901119901119867119867)minus(0633times1199011199011198671198672)minus[0264times119901119901119867119867ln(ℎ119886119886119903119903119889119889)]]

ndash 119875119875 119901119901119903119903119900119900119898119898119890119890119897119897119886119886119904119904 11986411986411986211986220=119890119890[minus14029+[0503timesln(119863119863119874119874119862119862)]+[3443timesln(ℎ119886119886119903119903119889119889)]+(3131times119901119901119867119867)minus[0494times119901119901119867119867ln(ℎ119886119886119903119903119889119889)]]

bull These models were applied to both the acute and chronic aluminum toxicity datandash The C dubia model was applied to invertebrate datandash The P promelas model was applied to vertebrate data

19

III Normalize toxicity data in species sensitivity distribution to a set of water

quality conditionsbull Just like single parameter hardness models the MLR

models were used to normalize the aluminum toxicity data to a single set of water quality conditionsndash As an illustration for one set of calculations the draft

aluminum AWQC set these conditions to pH 7 hardness of 100 mgL and DOC of 1 mgL

bull This allows for comparisons to be made between species based on their sensitivity to aluminumndash Apples to apples

20

IV Construct sensitivity distribution to determine criteria at set conditions

21

Figure 8 Ranked Summary of Total Aluminum Genus Mean Acute Values (GMAVs) - Freshwater at pH 7 Hardness of 100 mgL and DOC of 1 mgL

IV Construct sensitivity distribution to determine criteria at set conditions

22

Figure 10 Ranked Summary of Total Aluminum Genus Mean Chronic Values (GMCVs) ndash Freshwater Supplemented with Other Data to Fulfill Missing MDRs at pH 7 Hardness of 100 mgL and DOC of 1 mgL

Aluminum Criteria Calculatorbull The relative ranking of genera in the sensitivity

distribution to which the toxicity data are normalized will change based on the set of water chemistry conditions (ie pH hardness and DOC)

bull To make calculating aluminum criteria easier EPA developed an Aluminum Criteria Calculatorndash Automated so that user only needs to enter water chemistry

conditions of interestndash Allows quick calculations for a variety of conditionsndash Limits calculations to the recommended conditions for which

the MLR models were developed were specified for hardness and DOC

bull No extrapolation beyond hardness and DOC empirical data bounds

23

Aluminum Criteria Calculator SD Example

24

pH=7 Hardness=100 DOC=1 pH=6 Hardness=25 DOC=1

Chronic Ranked GMCVRank GMCV Genus

12 17098 Aeolosoma Invert11 gt 9746 Rana Amphib - Other Data10 5908 Chironomus Invert

9 4877 Lymnaea Mollusk8 2555 Brachionus Invert7 2488 Pimephales Fish6 1713 Hyalella Invert5 1182 Ceriodaphnia Invert4 1102 Danio Fish3 1042 Lampsilis Mollusk2 591 Salvelinus Fish1 509 Salmo Fish

FCV = 3940 microgL total aluminum

Chronic Ranked GMCVRank GMCV Genus

12 gt 2131 Rana Amphib - Other Data11 1710 Aeolosoma Invert10 591 Chironomus Invert

9 544 Pimephales Fish8 488 Lymnaea Mollusk7 255 Brachionus Invert6 241 Danio Fish5 171 Hyalella Invert4 129 Salvelinus Fish3 118 Ceriodaphnia Invert2 111 Salmo Fish1 104 Lampsilis Mollusk

FCV = 987 microgL total aluminum

Multiple Scenarios

Run Scenarios

Summary Sheet

Acute Dataset

Chronic Dataset

Multiple Scenarios

Run Scenarios

Summary Sheet

Acute Dataset

Chronic Dataset

Boundaries of the Criteria Outputsbull MLR models were developed using data that

encompassndash DOC range of 008-5 mgLndash Hardness range of 98-127 mgL (as CaCO3)ndash pH range of 6-81

bull MLR criteria outputs are bounded atndash Maximum of 150 mgL total hardness (as CaCO3)

ndash DOC of 50 mgLndash pH range of the model is 50 to 90

25

Aluminum Criteria Look-Up Tables

26

bull EPA also provided criteria look-up tables in Appendix K

Har

dnes

s

CCC(microgL total aluminum)

(DOC=05 mgL)

pH 50 pH 55 pH 60 pH 65 pH 70 pH 75 pH 80 pH 85 pH 90

25 23 a 14 b 69 c 160 f 350 f 620 i 540 b 410 a 230 a

50 43 a 24 b 100 d 170 f 310 f 630 f 670 j 400 a 200 a

75 61 a 33 b 130 e 180 f 290 f 530 f 730 i 390 a 190 a

100 78 a 41 b 150 e 190 f 280 g 460 f 770 c 390 a 180 a

150 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

200 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

250 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

300 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

350 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

400 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

Aluminum Criteria Look-Up Tables

27

bull EPA also provided criteria look-up tables in Appendix K

Har

dnes

s

CCC(microgL total aluminum)

(DOC=10 mgL)

pH 50 pH 55 pH 60 pH 65 pH 70 pH 75 pH 80 pH 85 pH 90

25 33 a 21 b 99 c 220 f 490 f 900 i 780 b 590 a 330 a

50 61 a 35 b 150 e 250 f 440 f 890 f 960 j 580 a 290 a

75 88 a 47 b 180 e 260 f 410 f 740 f 1100 i 570 a 270 a

100 11 a 59 b 210 e 270 f 390 g 650 f 1100 c 560 b 260 a

150 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

200 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

250 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

300 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

350 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

400 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

Aluminum Criteria Look-Up Tables

28

bull EPA also provided criteria look-up tables in Appendix K

Har

dnes

s

CCC(microgL total aluminum)

(DOC=25 mgL)

pH 50 pH 55 pH 60 pH 65 pH 70 pH 75 pH 80 pH 85 pH 90

25 54 a 34 b 160 c 350 f 770 f 1500 i 1300 b 960 a 540 a

50 99 a 57 b 230 e 390 f 690 f 1400 f 1600 j 930 a 470 a

75 14 a 77 b 290 e 410 f 640 f 1200 f 1700 i 920 a 440 a

100 18 a 96 b 330 e 420 f 620 g 1000 f 1800 d 920 b 420 a

150 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

200 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

250 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

300 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

350 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

400 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

Aluminum Criteria Look-Up Tables

29

bull EPA also provided criteria look-up tables in Appendix K

Har

dnes

s

CCC(microgL total aluminum)

(DOC=50 mgL)

pH 50 pH 55 pH 60 pH 65 pH 70 pH 75 pH 80 pH 85 pH 90

25 77 a 49 b 230 c 500 f 1100 f 2100 c 1900 b 1400 a 770 a

50 14 a 82 b 330 e 540 f 970 f 2000 f 2200 j 1300 a 680 a

75 20 a 110 b 410 e 570 f 900 f 1600 f 2500 i 1300 a 640 a

100 26 a 140 b 460 f 590 f 880 g 1400 f 2500 d 1300 b 600 a

150 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

200 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

250 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

300 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

350 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

400 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

Status

30

bull Draft Document Released to the Federal Register on 72817ndash Public comment period of 60 days ndash Due Date for Comments is 92617

bull Revisions will be made considering public commentsbull Subsequent EPA intra-agency review of final criteria

document will then be conducted bull Publication of final criteria document planned for Late Fall

2017

Federal Register Webpagehttpswwwfederalregistergovdocuments201

707282017-15968request-for-scientific-views-draft-updated-aquatic-life-ambient-water-quality-criteria-for-aluminum

Aluminum Criteria Webpagehttpswwwepagovwqc2017-draft-aquatic-life-

criteria-aluminum-freshwater-documents

31

	Appendix C Acceptable Chronic Toxicity Data of Aluminum to Freshwater Aquatic Animals
									MLR equation
	Species		Hardness (mgL as CaCO3)		pH		DOC(mgL)		EC20 Endpoint			EC20(microgL)			Normalized Conc- EC20(microgL)			SMCV-EC20(microgL)		GMCV-EC20(microgL)			Genus			DOC Notes		Dilution water
	Oligochaete Aeolosoma sp		48		6		025		Reproduction (population size)			1235			1710			1710		1710			Aeolosoma		Invert		Author reported half the detection limit
	Rotifer Brachionus calyciflorus		100		62		025		Reproduction (population size)			431			255			255		255			Brachionus		Invert		Author reported half the detection limit
	Great pond snail Lymnaea stagnalis		117		61		025		Biomass			746			488			488		488			Lymnaea		Mollusk		Author reported half the detection limit
	Fatmucket Lampsilis siliquoidea		1055		604		040		Biomass			169			104			104		104			Lampsilis		Mollusk		Author reported
	Cladoceran Ceriodaphnia dubia		50		715		11		Reproduction - youngstarting adult			1780			190						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		26		73		05		Reproduction - youngfemale			1557			272						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		46		75		05		Reproduction - youngfemale			8087			110						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		96		79		05		Reproduction - youngfemale			6519			80						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		194		81		05		Reproduction - youngfemale			6836			89						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		255		703		05		Reproduction (youngfemale)			250			56						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		122		713		05		Reproduction (youngfemale)			860			106						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		25		798		05		Reproduction (youngfemale)			700			101						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		61		803		05		Reproduction (youngfemale)			1010			136						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		122		810		05		Reproduction (youngfemale)			870			116						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		255		633		05		Reproduction (youngfemale)			260			176						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		121		636		05		Reproduction (youngfemale)			390			110			118		118			Ceriodaphnia		Invert		Author reported
	Amphipod Hyalella azteca		95		61		051		Biomass (28 d)			1993			101						Hyalella			Author reported
	Amphipod Hyalella azteca		106		604		033		Biomass (28 d)			425			289			171		171			Hyalella		Invert		Author reported
	Midge Chironomus riparius		118		558		18		Adult midge emergence			2955			119						Chironomus			Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Midge Chironomus riparius		119		505		18		Adult midge emergence			8442			2258						Chironomus			Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Midge Chironomus riparius		91		66		051		Reproduction ( of eggscase)			3387			766			591		591			Chironomus		Invert		Author reported
	Atlantic salmon Salmo salar		127		57		18		Biomass			6156			1112			1112		1112			Salmo		Fish		Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Brook trout Salvelinus fontinalis		123		655		19		Biomass			1644			59						Salvelinus			Author reported		wellRO mix
	Brook trout Salvelinus fontinalis		128		565		18		Biomass			1435			283			1293		1293			Salvelinus		Fish		Author reported		wellRO mix
	Fathead minnow Pimephales promelas		220		770		16		Biomass			6194			781						Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		well water
	Fathead minnow Pimephales promelas		96		620		025		Fry survival			4286			379			544		544			Pimephales		Fish		Author reported half the detection limit
	Zebrafish Danio rerio		83		61		025		Biomass			2344			241			241		241			Danio		Fish		Author reported half the detection limit
	Wood frog Rana sylvatica		115		469		16		MATC-survival		gt		2000		gt		2131		gt		2131		2131		gt		Rana		Amphib - Other Data		Value is from 2007 FW Cu AWQC Appendix C recommendation		aged tap water

	Appendix A Acceptable Acute Toxicity Data of Aluminum to Freshwater Aquatic Animals
	Species		Methoda		Hardness (mgL as CaCO3)		pH		DOC(mgL)			LC50 or EC50 Conc (microgL)		Reference			Normalized Conc - LC50(microgL)			Species Mean Acute Value(microgL)		Genus Mean Acute Value(microgL)			Genus			DOC Note		Dilution water
	Snail Physa sp		S M T		474		659		11		gt		23400		Call 1984 Call et al 1984		gt		4843			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		755		11			30600		Call 1984 Call et al 1984			2699			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		817		11		gt		24700		Call 1984 Call et al 1984		gt		2404			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		746		11			55500		Call 1984 Call et al 1984			5034			3546		3546			Physa		Mollusk		Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Fatmucket Lampsilis siliquoidea		F M T		1060		612		048		gt		6302		Wang et al 2016 Wang et al 2017		gt		2983		gt		2983		2983		gt		Lampsilis		Mollusk		Author reported
	Cladoceran Ceriodaphnia dubia		S M A		500		742		11			1900		McCauley et al 1986			173			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		S M A		505		786		11			1500		McCauley et al 1986			129			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		UW-S Lake Superior lab water
	Cladoceran Ceriodaphnia dubia		S M A		500		813		11			2560		McCauley et al 1986			243			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		R M T		26		75		05			720		ENSR 1992d			111			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		R M T		46		76		05			1880		ENSR 1992d			250			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		R M T		96		78		05			2450		ENSR 1992d			289			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		S U NR		90		715		05			3727		Fort and Stover 1995			500			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U NR		90		715		05			5673		Fort and Stover 1995			761			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U NR		89		76		05			2880		Soucek et al 2001			336			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		601		05			7112		European Al Association 2009			169			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		605		2			6865		European Al Association 2009			709			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		609		4			15581		European Al Association 2009			1007			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		601		05			681		European Al Association 2009			162			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		603		05			1630		European Al Association 2009			367			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		597		05			1785		European Al Association 2009			473			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		592		05			1410		European Al Association 2009			429			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		699		05		gt		1300		European Al Association 2009		gt		419			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		785		05		gt		5000		European Al Association 2009		gt		796			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		68		2		gt		10000		European Al Association 2009		gt		2076			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		782		2		gt		15000		European Al Association 2009		gt		1163			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		677		4		gt		10000		European Al Association 2009		gt		1516			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		766		4		gt		15000		European Al Association 2009		gt		861			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		79		05		gt		2000		European Al Association 2009		gt		315			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		789		05		gt		2000		European Al Association 2009		gt		316			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		604		05			1108		European Al Association 2009			85			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		598		2			11371		European Al Association 2009			482			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		573		4			80467		European Al Association 2009			4357			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		671		05		gt		10000		European Al Association 2009		gt		2379			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		783		05		gt		5000		European Al Association 2009		gt		634			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		679		2		gt		10000		European Al Association 2009		gt		1039			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		767		2		gt		15000		European Al Association 2009		gt		908			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		668		4		gt		15000		European Al Association 2009		gt		1246			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		762		4		gt		15000		European Al Association 2009		gt		633			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		606		2			33868		European Al Association 2009			792			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		56		4			104842		European Al Association 2009			4880			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		693		05		gt		5000		European Al Association 2009		gt		713			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		120		788		05		gt		5000		European Al Association 2009		gt		590			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		120		676		2		gt		15000		European Al Association 2009		gt		1212			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		771		2		gt		15000		European Al Association 2009		gt		810			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		66		4		gt		15000		European Al Association 2009		gt		1014			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		76		4		gt		15000		European Al Association 2009		gt		555			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			11971		European Al Association 2010			270			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			27478		European Al Association 2010			619			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			11998		European Al Association 2010			270			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		607		05			92495		European Al Association 2010			188			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		609		05			31337		European Al Association 2010			604			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		61		05			33235		European Al Association 2010			624			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		708		05		gt		8864		European Al Association 2010		gt		253			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		779		05		gt		42783		European Al Association 2010		gt		693			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		753		05			13204		European Al Association 2010			24			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		600		601		05			46326		European Al Association 2010			380			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		600		599		05		gt		8590		European Al Association 2010		gt		737			509				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia reticulata		F M T		451		60		11			304		Shephard 1983			201			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia reticulata		F M T		40		55		11			362		Shephard 1983			5531			1054		733			Ceriodaphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake SuperiorDI Mix
	Cladoceran Daphnia magna		S U NR		453		774		11			3900		Biesinger and Christensen 1972			338			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Daphnia magna		S M T		220		76		16			38200		Kimball 1978			2030			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Well water
	Cladoceran Daphnia magna		S U T		451		725		11			2800		Shephard 1983			287			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Daphnia magna		S U T		168		599		05			5000		European Al Association 2009		gt		227			-				Daphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Daphnia magna		S U T		168		792		05			7950		European Al Association 2009			92			333		333			Daphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Ostracod Stenocypris major		R M T		1563		651		16			310196		Shuhaimi-Othman et al 2011a			1022			1022		1022			Stenocypris		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (Malaysia)
	Amphipod Crangonyx pseudogracilis		R U T		50		675		16			9190		Martin and Holdich 1986			1217			1217		1217			Crangonyx		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (UK)
	Amphipod Hyalella azteca		F M T		105		613		048		gt		5997		Wang et al 2016 Wang et al 2017		gt		2800		gt		2800		2800		gt		Hyalella		Invert		Author reported
	Stonefly Acroneuria sp		S M T		474		746		11		gt		22600		Call et al 1984		gt		2050		gt		2050		2050		gt		Acroneuria		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Midge Chironomus plumosus		S U T		80		70		16			30000		Fargasova 2001			2536			2536		2536			Chironomus		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (Slovakia)
	Midge Paratanytarsus dissimilis		S M T		1743		728		28		gt		77700		Lamb and Bailey 1981 1983		gt		6232		gt		6232		6232		gt		Paratanytarsus		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Liberty Lake Washington
	Rainbow trout Oncorhynchus mykiss		F M T		274		758		05		gt		9840		Gundersen et al 1994		gt		1256			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		452		762		05		gt		8070		Gundersen et al 1994		gt		1140			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		895		758		05		gt		8160		Gundersen et al 1994		gt		1489			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		1304		758		05		gt		8200		Gundersen et al 1994		gt		1676			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		232		825		05			6170		Gundersen et al 1994			260			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		350		826		05			6170		Gundersen et al 1994			333			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		836		829		05			7670		Gundersen et al 1994			701			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		1158		830		05			6930		Gundersen et al 1994			777			801		801			Oncorhynchus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Atlantic salmon Salmo salar		S U T		68		55		05			584		Hamilton and Haines 1995			4602			-				Salmo			Value is from 2007 FW Cu AWQC Appendix C recommendation		Soft recon
	Atlantic salmon Salmo salar		S U T		68		65		05			599		Hamilton and Haines 1995			531			1564		1564			Salmo		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Soft recon
	Brook trout Salvelinus fontinalis		S U T		40		56		16			6530		Tandjung 1982			6947			-				Salvelinus			Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Brook trout Salvelinus fontinalis		S U T		18		56		16			3400		Tandjung 1982			6209			-				Salvelinus			Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Brook trout Salvelinus fontinalis		S U T		2		56		16			370		Tandjung 1982			2988			5051		5051			Salvelinus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Green sunfish Lepomis cyanellus		S M T		474		755		11		gt		50000		Call et al 1984		gt		5255		gt		5255		5255		gt		Lepomis		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Rio Grande silvery minnow Hybognathus amarus		R M T		140		81		05		gt		59100		Buhl 2002		gt		8620		gt		8620		8620		gt		Hybognathus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon water
	Fathead minnow Pimephales promelas		S M T		474		761		11		gt		48200		Call et al 1984		gt		4707			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Fathead minnow Pimephales promelas		S M T		474		805		11		gt		49800		Call et al 1984		gt		2837			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Fathead minnow Pimephales promelas		R M T		26		78		05			1160		ENSR 1992c			104			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		46		76		05			8180		ENSR 1992c			1191			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		96		81		05			20300		ENSR 1992c			2398			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		194		81		05			44800		ENSR 1992c			7839			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		140		81		05		gt		59100		Buhl 2002		gt		8620			2224		2224			Pimephales		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon water
	Smallmouth bass Micropterus dolomieui		S M T		1245		505		16			130		Kane and Rabeni 1987			859			859		859			Micropterus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Green tree frog Hyla cinerea		R M T		455		549		05		gt		4052		Jung and Jagoe 1995		gt		4377		gt		4377		4377		gt		Hyla		Amphibian		Value is from 2007 FW Cu AWQC Appendix C recommendation		artificial soft recon

	Normalization Chemistry
	pH		6					FAV		360
	Hardness		25					CMC		180
	DOC		1					CCC		99
	Acute Ranked GMAV											Chronic Ranked GMCV
	Rank			GMAV		Genus								Rank			GMCV		Genus
	18		gt		8620		Hybognathus		Fish							12		gt		2131		Rana		Amphib - Other Data
	17		gt		6232		Paratanytarsus		Invert							11			1710		Aeolosoma		Invert
	16		gt		5255		Lepomis		Fish							10			591		Chironomus		Invert
	15			5051		Salvelinus		Fish							9			544		Pimephales		Fish
	14		gt		4377		Hyla		Amphibian							8			488		Lymnaea		Mollusk
	13			3546		Physa		Mollusk							7			255		Brachionus		Invert
	12		gt		2983		Lampsilis		Mollusk							6			241		Danio		Fish
	11		gt		2800		Hyalella		Invert							5			171		Hyalella		Invert
	10			2536		Chironomus		Invert							4			129		Salvelinus		Fish
	9			2224		Pimephales		Fish							3			118		Ceriodaphnia		Invert
	8		gt		2050		Acroneuria		Invert							2			111		Salmo		Fish
	7			1564		Salmo		Fish							1			104		Lampsilis		Mollusk
	6			1217		Crangonyx		Invert
	5			1022		Stenocypris		Invert
	4			859		Micropterus		Fish
	3			801		Oncorhynchus		Fish
	2			733		Ceriodaphnia		Invert
	1			333		Daphnia		Invert
		N		Rank		Genus		GMAV		ln(GMAV)		ln(GMAV)2		P=R(N+1)		sqrt(P)				N		Rank		Genus		GMCV		ln(GMCV)		ln(GMCV)2		P=R(N+1)		sqrt(P)
		18		4		Micropterus		859		676		4564		0211		0459				12		4		Salvelinus		129		486		2364		0308		0555
			3		Oncorhynchus		801		669		4469		0158		0397					3		Ceriodaphnia		118		477		2277		0231		0480
			2		Ceriodaphnia		733		660		4352		0105		0324					2		Salmo		111		471		2220		0154		0392
			1		Daphnia		333		581		3374		0053		0229					1		Lampsilis		104		465		2159		0077		0277
			Sum				2585		1676		0526		141					Sum				1899		902		0769		170
			S2 =		1987										S2 =		059
			L =		4890										L =		4420
			A =		5887										A =		4592
			FAV =		360										FCV =		987
			CMC =		180

				All concentrations reported are microgL total Aluminum
				lt-----Click Button after entering data
	ENTER DATA HERE									ERRORNUM		ACUTE														CHRONIC
										Rank 1				Rank 2				Rank 3				Rank 4					Rank 1				Rank 2				Rank 3				Rank 4
	SiteName		DOC(mgL)		Hardness(mgL as CaCO3)		pH			FAV		CMC		CCC			Genus		GMAV			Genus		GMAV			Genus		GMAV			Genus		GMAV				Genus		GMCV			Genus		GMCV			Genus		GMCV			Genus		GMCV
	Site 1		50		25		9		75428737034162		35229324666008		1800		770			Daphnia			33452		Ceriodaphnia			73576		Stenocypris			102576		Crangonyx			122230			Lampsilis			10463		Ceriodaphnia			11865		Hyalella			17200		Brachionus			25648
	Site 2		50		50		9		75428737034162		31204991509063		1600		680			Daphnia			29631		Ceriodaphnia			65171		Stenocypris			90859		Crangonyx			108267			Lampsilis			9268		Ceriodaphnia			10510		Hyalella			15236		Brachionus			22718
	Site 3		50		75		9		75428737034162		29067527928199		1500		640			Daphnia			27601		Ceriodaphnia			60707		Stenocypris			84635		Crangonyx			100851			Lampsilis			8633		Ceriodaphnia			9790		Hyalella			14192		Brachionus			21162
	Site 4		50		100		9		75428737034162		27640367912592		1400		600			Daphnia			26246		Ceriodaphnia			57726		Stenocypris			80480		Crangonyx			95899			Lampsilis			8209		Ceriodaphnia			9309		Hyalella			13495		Brachionus			20123
	Site 5		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 6		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 7		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 8		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 9		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 10		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 11		50		25		85		75428737034162		63245275664384		3200		1400			Daphnia			60055		Ceriodaphnia			132086		Stenocypris			184149		Crangonyx			219432			Lampsilis			18783		Ceriodaphnia			21301		Hyalella			30879		Brachionus			46045
	Site 12		50		50		85		75428737034162		61388044609926		3100		1300			Daphnia			58291		Ceriodaphnia			128208		Stenocypris			178742		Crangonyx			212988			Lampsilis			18232		Ceriodaphnia			20676		Hyalella			29972		Brachionus			44693
	Site 13		50		75		85		75428737034162		60327020429132		3000		1300			Daphnia			57284		Ceriodaphnia			125992		Stenocypris			175652		Crangonyx			209307			Lampsilis			17917		Ceriodaphnia			20318		Hyalella			29454		Brachionus			43920
	Site 14		50		100		85		75428737034162		59585352130146		3000		1300			Daphnia			56580		Ceriodaphnia			124443		Stenocypris			173493		Crangonyx			206734			Lampsilis			17696		Ceriodaphnia			20068		Hyalella			29092		Salmo			41264
	Site 15		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 16		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 17		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 18		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 19		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 20		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370

	Appendix C Acceptable Chronic Toxicity Data of Aluminum to Freshwater Aquatic Animals
									MLR equation
	Species		Hardness (mgL as CaCO3)		pH		DOC(mgL)		EC20 Endpoint			EC20(microgL)			Normalized Conc- EC20(microgL)			SMCV-EC20(microgL)		GMCV-EC20(microgL)			Genus			DOC Notes		Dilution water
	Oligochaete Aeolosoma sp		48		6		025		Reproduction (population size)			1235			17098			17098		17098			Aeolosoma		Invert		Author reported half the detection limit
	Rotifer Brachionus calyciflorus		100		62		025		Reproduction (population size)			431			2555			2555		2555			Brachionus		Invert		Author reported half the detection limit
	Great pond snail Lymnaea stagnalis		117		61		025		Biomass			746			4877			4877		4877			Lymnaea		Mollusk		Author reported half the detection limit
	Fatmucket Lampsilis siliquoidea		1055		604		040		Biomass			169			1042			1042		1042			Lampsilis		Mollusk		Author reported
	Cladoceran Ceriodaphnia dubia		50		715		11		Reproduction - youngstarting adult			1780			1898						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		26		73		05		Reproduction - youngfemale			1557			2718						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		46		75		05		Reproduction - youngfemale			8087			1102						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		96		79		05		Reproduction - youngfemale			6519			796						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		194		81		05		Reproduction - youngfemale			6836			888						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		255		703		05		Reproduction (youngfemale)			250			563						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		122		713		05		Reproduction (youngfemale)			860			1061						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		25		798		05		Reproduction (youngfemale)			700			1006						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		61		803		05		Reproduction (youngfemale)			1010			1363						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		122		810		05		Reproduction (youngfemale)			870			1164						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		255		633		05		Reproduction (youngfemale)			260			1758						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		121		636		05		Reproduction (youngfemale)			390			1101			1182		1182			Ceriodaphnia		Invert		Author reported
	Amphipod Hyalella azteca		95		61		051		Biomass (28 d)			1993			1014						Hyalella			Author reported
	Amphipod Hyalella azteca		106		604		033		Biomass (28 d)			425			2895			1713		1713			Hyalella		Invert		Author reported
	Midge Chironomus riparius		118		558		18		Adult midge emergence			2955			1192						Chironomus			Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Midge Chironomus riparius		119		505		18		Adult midge emergence			8442			22578						Chironomus			Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Midge Chironomus riparius		91		66		051		Reproduction ( of eggscase)			3387			7664			5908		5908			Chironomus		Invert		Author reported
	Atlantic salmon Salmo salar		127		57		18		Biomass			6156			5085			5085		5085			Salmo		Fish		Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Brook trout Salvelinus fontinalis		123		655		19		Biomass			1644			270						Salvelinus			Author reported		wellRO mix
	Brook trout Salvelinus fontinalis		128		565		18		Biomass			1435			1295			5914		5914			Salvelinus		Fish		Author reported		wellRO mix
	Fathead minnow Pimephales promelas		220		770		16		Biomass			6194			3569						Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		well water
	Fathead minnow Pimephales promelas		96		620		025		Fry survival			4286			1734			2488		2488			Pimephales		Fish		Author reported half the detection limit
	Zebrafish Danio rerio		83		61		025		Biomass			2344			1102			1102		1102			Danio		Fish		Author reported half the detection limit
	Wood frog Rana sylvatica		115		469		16		MATC-survival		gt		2000		gt		9746		gt		9746		9746		gt		Rana		Amphib - Other Data		Value is from 2007 FW Cu AWQC Appendix C recommendation		aged tap water

	Appendix A Acceptable Acute Toxicity Data of Aluminum to Freshwater Aquatic Animals
	Species		Methoda		Hardness (mgL as CaCO3)		pH		DOC(mgL)			LC50 or EC50 Conc (microgL)		Reference			Normalized Conc - LC50(microgL)			Species Mean Acute Value(microgL)		Genus Mean Acute Value(microgL)			Genus			DOC Note		Dilution water
	Snail Physa sp		S M T		474		659		11		gt		23400		Call 1984 Call et al 1984		gt		48425			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		755		11			30600		Call 1984 Call et al 1984			26986			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		817		11		gt		24700		Call 1984 Call et al 1984		gt		24040			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		746		11			55500		Call 1984 Call et al 1984			50338			35462		35462			Physa		Mollusk		Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Fatmucket Lampsilis siliquoidea		F M T		1060		612		048		gt		6302		Wang et al 2016 Wang et al 2017		gt		29834		gt		29834		29834		gt		Lampsilis		Mollusk		Author reported
	Cladoceran Ceriodaphnia dubia		S M A		500		742		11			1900		McCauley et al 1986			1728			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		S M A		505		786		11			1500		McCauley et al 1986			1294			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		UW-S Lake Superior lab water
	Cladoceran Ceriodaphnia dubia		S M A		500		813		11			2560		McCauley et al 1986			2431			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		R M T		26		75		05			720		ENSR 1992d			1113			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		R M T		46		76		05			1880		ENSR 1992d			2495			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		R M T		96		78		05			2450		ENSR 1992d			2894			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		S U NR		90		715		05			3727		Fort and Stover 1995			5002			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U NR		90		715		05			5673		Fort and Stover 1995			7613			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U NR		89		76		05			2880		Soucek et al 2001			3362			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		601		05			7112		European Al Association 2009			1691			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		605		2			6865		European Al Association 2009			7088			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		609		4			15581		European Al Association 2009			10072			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		601		05			681		European Al Association 2009			1620			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		603		05			1630		European Al Association 2009			3674			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		597		05			1785		European Al Association 2009			4732			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		592		05			1410		European Al Association 2009			4295			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		699		05		gt		1300		European Al Association 2009		gt		4189			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		785		05		gt		5000		European Al Association 2009		gt		7960			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		68		2		gt		10000		European Al Association 2009		gt		20755			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		782		2		gt		15000		European Al Association 2009		gt		11626			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		677		4		gt		10000		European Al Association 2009		gt		15161			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		766		4		gt		15000		European Al Association 2009		gt		8608			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		79		05		gt		2000		European Al Association 2009		gt		3150			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		789		05		gt		2000		European Al Association 2009		gt		3156			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		604		05			1108		European Al Association 2009			850			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		598		2			11371		European Al Association 2009			4817			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		573		4			80467		European Al Association 2009			43570			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		671		05		gt		10000		European Al Association 2009		gt		23786			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		783		05		gt		5000		European Al Association 2009		gt		6344			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		679		2		gt		10000		European Al Association 2009		gt		10394			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		767		2		gt		15000		European Al Association 2009		gt		9082			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		668		4		gt		15000		European Al Association 2009		gt		12461			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		762		4		gt		15000		European Al Association 2009		gt		6332			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		606		2			33868		European Al Association 2009			7920			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		56		4			104842		European Al Association 2009			48800			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		693		05		gt		5000		European Al Association 2009		gt		7126			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		120		788		05		gt		5000		European Al Association 2009		gt		5898			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		120		676		2		gt		15000		European Al Association 2009		gt		12117			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		771		2		gt		15000		European Al Association 2009		gt		8096			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		66		4		gt		15000		European Al Association 2009		gt		10139			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		76		4		gt		15000		European Al Association 2009		gt		5545			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			11971		European Al Association 2010			2699			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			27478		European Al Association 2010			6194			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			11998		European Al Association 2010			2705			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		607		05			92495		European Al Association 2010			1876			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		609		05			31337		European Al Association 2010			6035			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		61		05			33235		European Al Association 2010			6238			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		708		05		gt		8864		European Al Association 2010		gt		2534			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		779		05		gt		42783		European Al Association 2010		gt		6930			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		753		05			13204		European Al Association 2010			244			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		600		601		05			46326		European Al Association 2010			3798			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		600		599		05		gt		8590		European Al Association 2010		gt		7366			5094				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia reticulata		F M T		451		60		11			304		Shephard 1983			2009			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia reticulata		F M T		40		55		11			362		Shephard 1983			55307			10542		7328			Ceriodaphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake SuperiorDI Mix
	Cladoceran Daphnia magna		S U NR		453		774		11			3900		Biesinger and Christensen 1972			3382			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Daphnia magna		S M T		220		76		16			38200		Kimball 1978			20303			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Well water
	Cladoceran Daphnia magna		S U T		451		725		11			2800		Shephard 1983			2868			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Daphnia magna		S U T		168		599		05			5000		European Al Association 2009		gt		2265			-				Daphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Daphnia magna		S U T		168		792		05			7950		European Al Association 2009			920			3332		3332			Daphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Ostracod Stenocypris major		R M T		1563		651		16			310196		Shuhaimi-Othman et al 2011a			10216			10216		10216			Stenocypris		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (Malaysia)
	Amphipod Crangonyx pseudogracilis		R U T		50		675		16			9190		Martin and Holdich 1986			12174			12174		12174			Crangonyx		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (UK)
	Amphipod Hyalella azteca		F M T		105		613		048		gt		5997		Wang et al 2016 Wang et al 2017		gt		28002		gt		28002		28002		gt		Hyalella		Invert		Author reported
	Stonefly Acroneuria sp		S M T		474		746		11		gt		22600		Call et al 1984		gt		20498		gt		20498		20498		gt		Acroneuria		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Midge Chironomus plumosus		S U T		80		70		16			30000		Fargasova 2001			25361			25361		25361			Chironomus		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (Slovakia)
	Midge Paratanytarsus dissimilis		S M T		1743		728		28		gt		77700		Lamb and Bailey 1981 1983		gt		62318		gt		62318		62318		gt		Paratanytarsus		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Liberty Lake Washington
	Rainbow trout Oncorhynchus mykiss		F M T		274		758		05		gt		9840		Gundersen et al 1994		gt		5745			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		452		762		05		gt		8070		Gundersen et al 1994		gt		5212			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		895		758		05		gt		8160		Gundersen et al 1994		gt		6807			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		1304		758		05		gt		8200		Gundersen et al 1994		gt		7662			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		232		825		05			6170		Gundersen et al 1994			1190			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		350		826		05			6170		Gundersen et al 1994			1523			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		836		829		05			7670		Gundersen et al 1994			3205			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		1158		830		05			6930		Gundersen et al 1994			3554			3661		3661			Oncorhynchus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Atlantic salmon Salmo salar		S U T		68		55		05			584		Hamilton and Haines 1995			21042			-				Salmo			Value is from 2007 FW Cu AWQC Appendix C recommendation		Soft recon
	Atlantic salmon Salmo salar		S U T		68		65		05			599		Hamilton and Haines 1995			2430			7151		7151			Salmo		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Soft recon
	Brook trout Salvelinus fontinalis		S U T		40		56		16			6530		Tandjung 1982			31767			-				Salvelinus			Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Brook trout Salvelinus fontinalis		S U T		18		56		16			3400		Tandjung 1982			28391			-				Salvelinus			Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Brook trout Salvelinus fontinalis		S U T		2		56		16			370		Tandjung 1982			13663			23097		23097			Salvelinus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Green sunfish Lepomis cyanellus		S M T		474		755		11		gt		50000		Call et al 1984		gt		24028		gt		24028		24028		gt		Lepomis		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Rio Grande silvery minnow Hybognathus amarus		R M T		140		81		05		gt		59100		Buhl 2002		gt		39414		gt		39414		39414		gt		Hybognathus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon water
	Fathead minnow Pimephales promelas		S M T		474		761		11		gt		48200		Call et al 1984		gt		21522			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Fathead minnow Pimephales promelas		S M T		474		805		11		gt		49800		Call et al 1984		gt		12972			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Fathead minnow Pimephales promelas		R M T		26		78		05			1160		ENSR 1992c			477			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		46		76		05			8180		ENSR 1992c			5446			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		96		81		05			20300		ENSR 1992c			10966			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		194		81		05			44800		ENSR 1992c			35847			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		140		81		05		gt		59100		Buhl 2002		gt		39414			10168		10168			Pimephales		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon water
	Smallmouth bass Micropterus dolomieui		S M T		1245		505		16			130		Kane and Rabeni 1987			3929			3929		3929			Micropterus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Green tree frog Hyla cinerea		R M T		455		549		05		gt		4052		Jung and Jagoe 1995		gt		20016		gt		20016		20016		gt		Hyla		Amphibian		Value is from 2007 FW Cu AWQC Appendix C recommendation		artificial soft recon

	Normalization Chemistry
	pH		7					FAV		2741
	Hardness		100					CMC		1400
	DOC		1					CCC		390
	Acute Ranked GMAV											Chronic Ranked GMCV
	Rank			GMAV		Genus								Rank			GMCV		Genus
	18		gt		62318		Paratanytarsus		Invert							12			17098		Aeolosoma		Invert
	17		gt		39414		Hybognathus		Fish							11		gt		9746		Rana		Amphib - Other Data
	16			35462		Physa		Mollusk							10			5908		Chironomus		Invert
	15		gt		29834		Lampsilis		Mollusk							9			4877		Lymnaea		Mollusk
	14		gt		28002		Hyalella		Invert							8			2555		Brachionus		Invert
	13			25361		Chironomus		Invert							7			2488		Pimephales		Fish
	12		gt		24028		Lepomis		Fish							6			1713		Hyalella		Invert
	11			23097		Salvelinus		Fish							5			1182		Ceriodaphnia		Invert
	10		gt		20498		Acroneuria		Invert							4			1102		Danio		Fish
	9		gt		20016		Hyla		Amphibian							3			1042		Lampsilis		Mollusk
	8			12174		Crangonyx		Invert							2			591		Salvelinus		Fish
	7			10216		Stenocypris		Invert							1			509		Salmo		Fish
	6			10168		Pimephales		Fish
	5			7328		Ceriodaphnia		Invert
	4			7151		Salmo		Fish
	3			3929		Micropterus		Fish
	2			3661		Oncorhynchus		Fish
	1			3332		Daphnia		Invert
		N		Rank		Genus		GMAV		ln(GMAV)		ln(GMAV)2		P=R(N+1)		sqrt(P)				N		Rank		Genus		GMCV		ln(GMCV)		ln(GMCV)2		P=R(N+1)		sqrt(P)
		18		4		Salmo		7151		887		7876		0211		0459				12		4		Danio		1102		700		4907		0308		0555
			3		Micropterus		3929		828		6849		0158		0397					3		Lampsilis		1042		695		4829		0231		0480
			2		Oncorhynchus		3661		821		6733		0105		0324					2		Salvelinus		591		638		4074		0154		0392
			1		Daphnia		3332		811		6579		0053		0229					1		Salmo		509		623		3883		0077		0277
			Sum				3347		2804		0526		141					Sum				2657		1769		0769		170
			S2 =		1223										S2 =		1080
			L =		7134										L =		5242
			A =		7916										A =		5976
			FAV =		2741										FCV =		3940
			CMC =		1371

				All concentrations reported are microgL total Aluminum
				lt-----Click Button after entering data
	ENTER DATA HERE									ERRORNUM		ACUTE														CHRONIC
										Rank 1				Rank 2				Rank 3				Rank 4					Rank 1				Rank 2				Rank 3				Rank 4
	SiteName		DOC(mgL)		Hardness(mgL as CaCO3)		pH			FAV		CMC		CCC			Genus		GMAV			Genus		GMAV			Genus		GMAV			Genus		GMAV				Genus		GMCV			Genus		GMCV			Genus		GMCV			Genus		GMCV
	Site 1		50		25		9		75428737034162		35229324666008		1800		770			Daphnia			33452		Ceriodaphnia			73576		Stenocypris			102576		Crangonyx			122230			Lampsilis			10463		Ceriodaphnia			11865		Hyalella			17200		Brachionus			25648
	Site 2		50		50		9		75428737034162		31204991509063		1600		680			Daphnia			29631		Ceriodaphnia			65171		Stenocypris			90859		Crangonyx			108267			Lampsilis			9268		Ceriodaphnia			10510		Hyalella			15236		Brachionus			22718
	Site 3		50		75		9		75428737034162		29067527928199		1500		640			Daphnia			27601		Ceriodaphnia			60707		Stenocypris			84635		Crangonyx			100851			Lampsilis			8633		Ceriodaphnia			9790		Hyalella			14192		Brachionus			21162
	Site 4		50		100		9		75428737034162		27640367912592		1400		600			Daphnia			26246		Ceriodaphnia			57726		Stenocypris			80480		Crangonyx			95899			Lampsilis			8209		Ceriodaphnia			9309		Hyalella			13495		Brachionus			20123
	Site 5		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 6		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 7		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 8		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 9		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 10		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 11		50		25		85		75428737034162		63245275664384		3200		1400			Daphnia			60055		Ceriodaphnia			132086		Stenocypris			184149		Crangonyx			219432			Lampsilis			18783		Ceriodaphnia			21301		Hyalella			30879		Brachionus			46045
	Site 12		50		50		85		75428737034162		61388044609926		3100		1300			Daphnia			58291		Ceriodaphnia			128208		Stenocypris			178742		Crangonyx			212988			Lampsilis			18232		Ceriodaphnia			20676		Hyalella			29972		Brachionus			44693
	Site 13		50		75		85		75428737034162		60327020429132		3000		1300			Daphnia			57284		Ceriodaphnia			125992		Stenocypris			175652		Crangonyx			209307			Lampsilis			17917		Ceriodaphnia			20318		Hyalella			29454		Brachionus			43920
	Site 14		50		100		85		75428737034162		59585352130146		3000		1300			Daphnia			56580		Ceriodaphnia			124443		Stenocypris			173493		Crangonyx			206734			Lampsilis			17696		Ceriodaphnia			20068		Hyalella			29092		Salmo			41264
	Site 15		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 16		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 17		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 18		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 19		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 20		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370

14

Minimum Dataset For Freshwater Criteria Derivation

SALMONID SECONDFISHFAMILY

CHORDATA

PLANKTONICCRUSTACEAN

BENTHIC CRUSTACEAN

INSECT ROTIFERA ANNELIDA MOLLUSCA

OTHERINSECT ORMOLLUSCA

EstuarineMarine Minimum Data Requirements

15

Family Minimum Data Requirement EstuarineMarine

Acute(Phylum Family Genus)

Chronic(Phylum Family Genus)

Family in the phylum Chordata - -

Family in the phylum Chordata - -

Either the Mysidae or Penaeidae family - -

Family in a phylum other than Arthropoda or Chordata Mollusca Ostreidae Crassostrea -

Family in a phylum other than Chordata Annelida Nereididae Neanthes -

Family in a phylum other than Chordata Annelida Capitellidae Capitella -

Family in a phylum other than Chordata Annelida Ctenodrilidae Ctenodrilus -

Any other family Arthropoda Ameiridae Nitokra -

II Evaluate water quality conditions to determine applicability

bull Criteria are often based on one or more water quality conditions (eg hardness) that affect toxicity of the material to test organisms

bull Aluminum toxicity is highly dependent on the pH of the test waterndash The pH of test waters affects the amount and form

of aluminum that is bioavailable to test speciesndash Hardness and DOC (Dissolved Organic Carbon)

also influence the amount of aluminum that is bioavailable

16

Multiple Bioavailability Modeling Approaches Underwent External Peer Review

17

bull Biotic Ligand Modelsbull Full aluminum BLM (2016 model version) ndash Temperature pH

Dissolved organic carbon (DOC) Major cations (calcium [Ca] magnesium [Mg] sodium [Na] and potassium [K]) Major anions (sulfate [SO4] and chloride [Cl]) Alkalinity and Sulfide

bull Simplified aluminum BLM - pH hardness DOC temperature

bull Multilinear Regression Equationsbull pH and Hardness

Accounting for Effects of Water Chemistry on Bioavailability and Toxicity

bull Water quality criteria can be modified quantitatively when enough data are available to demonstrate that those conditions similarly affect the results with a variety of speciesndash High quality data in the draft aluminum document demonstrated

that pH DOC and hardness affected a variety of species in a similar way when exposed to aluminum concentrations

bull Recent publications by Cardwell et al (2017) and Gensemer et al (2017) summarized short-term aluminum chronic toxicity data across a range of DOC pH and hardness values for three species

18

Multiple Linear Regression (MLR) Models to Account for Effects of Water Chemistry

bull MLR models were developed for each species (DeForest et al 2017)ndash 119862119862 119889119889119906119906119887119887119894119894119886119886 11986411986411986211986220=119890119890[minus41026+[0525timesln(119863119863119874119874119862119862)]+[2201timesln(ℎ119886119886119903119903119889119889)]+(11282times119901119901119867119867)minus(0633times1199011199011198671198672)minus[0264times119901119901119867119867ln(ℎ119886119886119903119903119889119889)]]

ndash 119875119875 119901119901119903119903119900119900119898119898119890119890119897119897119886119886119904119904 11986411986411986211986220=119890119890[minus14029+[0503timesln(119863119863119874119874119862119862)]+[3443timesln(ℎ119886119886119903119903119889119889)]+(3131times119901119901119867119867)minus[0494times119901119901119867119867ln(ℎ119886119886119903119903119889119889)]]

bull These models were applied to both the acute and chronic aluminum toxicity datandash The C dubia model was applied to invertebrate datandash The P promelas model was applied to vertebrate data

19

III Normalize toxicity data in species sensitivity distribution to a set of water

quality conditionsbull Just like single parameter hardness models the MLR

models were used to normalize the aluminum toxicity data to a single set of water quality conditionsndash As an illustration for one set of calculations the draft

aluminum AWQC set these conditions to pH 7 hardness of 100 mgL and DOC of 1 mgL

bull This allows for comparisons to be made between species based on their sensitivity to aluminumndash Apples to apples

20

IV Construct sensitivity distribution to determine criteria at set conditions

21

Figure 8 Ranked Summary of Total Aluminum Genus Mean Acute Values (GMAVs) - Freshwater at pH 7 Hardness of 100 mgL and DOC of 1 mgL

IV Construct sensitivity distribution to determine criteria at set conditions

22

Figure 10 Ranked Summary of Total Aluminum Genus Mean Chronic Values (GMCVs) ndash Freshwater Supplemented with Other Data to Fulfill Missing MDRs at pH 7 Hardness of 100 mgL and DOC of 1 mgL

Aluminum Criteria Calculatorbull The relative ranking of genera in the sensitivity

distribution to which the toxicity data are normalized will change based on the set of water chemistry conditions (ie pH hardness and DOC)

bull To make calculating aluminum criteria easier EPA developed an Aluminum Criteria Calculatorndash Automated so that user only needs to enter water chemistry

conditions of interestndash Allows quick calculations for a variety of conditionsndash Limits calculations to the recommended conditions for which

the MLR models were developed were specified for hardness and DOC

bull No extrapolation beyond hardness and DOC empirical data bounds

23

Aluminum Criteria Calculator SD Example

24

pH=7 Hardness=100 DOC=1 pH=6 Hardness=25 DOC=1

Chronic Ranked GMCVRank GMCV Genus

12 17098 Aeolosoma Invert11 gt 9746 Rana Amphib - Other Data10 5908 Chironomus Invert

9 4877 Lymnaea Mollusk8 2555 Brachionus Invert7 2488 Pimephales Fish6 1713 Hyalella Invert5 1182 Ceriodaphnia Invert4 1102 Danio Fish3 1042 Lampsilis Mollusk2 591 Salvelinus Fish1 509 Salmo Fish

FCV = 3940 microgL total aluminum

Chronic Ranked GMCVRank GMCV Genus

12 gt 2131 Rana Amphib - Other Data11 1710 Aeolosoma Invert10 591 Chironomus Invert

9 544 Pimephales Fish8 488 Lymnaea Mollusk7 255 Brachionus Invert6 241 Danio Fish5 171 Hyalella Invert4 129 Salvelinus Fish3 118 Ceriodaphnia Invert2 111 Salmo Fish1 104 Lampsilis Mollusk

FCV = 987 microgL total aluminum

Multiple Scenarios

Run Scenarios

Summary Sheet

Acute Dataset

Chronic Dataset

Multiple Scenarios

Run Scenarios

Summary Sheet

Acute Dataset

Chronic Dataset

Boundaries of the Criteria Outputsbull MLR models were developed using data that

encompassndash DOC range of 008-5 mgLndash Hardness range of 98-127 mgL (as CaCO3)ndash pH range of 6-81

bull MLR criteria outputs are bounded atndash Maximum of 150 mgL total hardness (as CaCO3)

ndash DOC of 50 mgLndash pH range of the model is 50 to 90

25

Aluminum Criteria Look-Up Tables

26

bull EPA also provided criteria look-up tables in Appendix K

Har

dnes

s

CCC(microgL total aluminum)

(DOC=05 mgL)

pH 50 pH 55 pH 60 pH 65 pH 70 pH 75 pH 80 pH 85 pH 90

25 23 a 14 b 69 c 160 f 350 f 620 i 540 b 410 a 230 a

50 43 a 24 b 100 d 170 f 310 f 630 f 670 j 400 a 200 a

75 61 a 33 b 130 e 180 f 290 f 530 f 730 i 390 a 190 a

100 78 a 41 b 150 e 190 f 280 g 460 f 770 c 390 a 180 a

150 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

200 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

250 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

300 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

350 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

400 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

Aluminum Criteria Look-Up Tables

27

bull EPA also provided criteria look-up tables in Appendix K

Har

dnes

s

CCC(microgL total aluminum)

(DOC=10 mgL)

pH 50 pH 55 pH 60 pH 65 pH 70 pH 75 pH 80 pH 85 pH 90

25 33 a 21 b 99 c 220 f 490 f 900 i 780 b 590 a 330 a

50 61 a 35 b 150 e 250 f 440 f 890 f 960 j 580 a 290 a

75 88 a 47 b 180 e 260 f 410 f 740 f 1100 i 570 a 270 a

100 11 a 59 b 210 e 270 f 390 g 650 f 1100 c 560 b 260 a

150 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

200 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

250 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

300 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

350 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

400 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

Aluminum Criteria Look-Up Tables

28

bull EPA also provided criteria look-up tables in Appendix K

Har

dnes

s

CCC(microgL total aluminum)

(DOC=25 mgL)

pH 50 pH 55 pH 60 pH 65 pH 70 pH 75 pH 80 pH 85 pH 90

25 54 a 34 b 160 c 350 f 770 f 1500 i 1300 b 960 a 540 a

50 99 a 57 b 230 e 390 f 690 f 1400 f 1600 j 930 a 470 a

75 14 a 77 b 290 e 410 f 640 f 1200 f 1700 i 920 a 440 a

100 18 a 96 b 330 e 420 f 620 g 1000 f 1800 d 920 b 420 a

150 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

200 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

250 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

300 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

350 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

400 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

Aluminum Criteria Look-Up Tables

29

bull EPA also provided criteria look-up tables in Appendix K

Har

dnes

s

CCC(microgL total aluminum)

(DOC=50 mgL)

pH 50 pH 55 pH 60 pH 65 pH 70 pH 75 pH 80 pH 85 pH 90

25 77 a 49 b 230 c 500 f 1100 f 2100 c 1900 b 1400 a 770 a

50 14 a 82 b 330 e 540 f 970 f 2000 f 2200 j 1300 a 680 a

75 20 a 110 b 410 e 570 f 900 f 1600 f 2500 i 1300 a 640 a

100 26 a 140 b 460 f 590 f 880 g 1400 f 2500 d 1300 b 600 a

150 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

200 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

250 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

300 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

350 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

400 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

Status

30

bull Draft Document Released to the Federal Register on 72817ndash Public comment period of 60 days ndash Due Date for Comments is 92617

bull Revisions will be made considering public commentsbull Subsequent EPA intra-agency review of final criteria

document will then be conducted bull Publication of final criteria document planned for Late Fall

2017

Federal Register Webpagehttpswwwfederalregistergovdocuments201

707282017-15968request-for-scientific-views-draft-updated-aquatic-life-ambient-water-quality-criteria-for-aluminum

Aluminum Criteria Webpagehttpswwwepagovwqc2017-draft-aquatic-life-

criteria-aluminum-freshwater-documents

31

	Appendix C Acceptable Chronic Toxicity Data of Aluminum to Freshwater Aquatic Animals
									MLR equation
	Species		Hardness (mgL as CaCO3)		pH		DOC(mgL)		EC20 Endpoint			EC20(microgL)			Normalized Conc- EC20(microgL)			SMCV-EC20(microgL)		GMCV-EC20(microgL)			Genus			DOC Notes		Dilution water
	Oligochaete Aeolosoma sp		48		6		025		Reproduction (population size)			1235			1710			1710		1710			Aeolosoma		Invert		Author reported half the detection limit
	Rotifer Brachionus calyciflorus		100		62		025		Reproduction (population size)			431			255			255		255			Brachionus		Invert		Author reported half the detection limit
	Great pond snail Lymnaea stagnalis		117		61		025		Biomass			746			488			488		488			Lymnaea		Mollusk		Author reported half the detection limit
	Fatmucket Lampsilis siliquoidea		1055		604		040		Biomass			169			104			104		104			Lampsilis		Mollusk		Author reported
	Cladoceran Ceriodaphnia dubia		50		715		11		Reproduction - youngstarting adult			1780			190						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		26		73		05		Reproduction - youngfemale			1557			272						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		46		75		05		Reproduction - youngfemale			8087			110						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		96		79		05		Reproduction - youngfemale			6519			80						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		194		81		05		Reproduction - youngfemale			6836			89						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		255		703		05		Reproduction (youngfemale)			250			56						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		122		713		05		Reproduction (youngfemale)			860			106						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		25		798		05		Reproduction (youngfemale)			700			101						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		61		803		05		Reproduction (youngfemale)			1010			136						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		122		810		05		Reproduction (youngfemale)			870			116						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		255		633		05		Reproduction (youngfemale)			260			176						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		121		636		05		Reproduction (youngfemale)			390			110			118		118			Ceriodaphnia		Invert		Author reported
	Amphipod Hyalella azteca		95		61		051		Biomass (28 d)			1993			101						Hyalella			Author reported
	Amphipod Hyalella azteca		106		604		033		Biomass (28 d)			425			289			171		171			Hyalella		Invert		Author reported
	Midge Chironomus riparius		118		558		18		Adult midge emergence			2955			119						Chironomus			Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Midge Chironomus riparius		119		505		18		Adult midge emergence			8442			2258						Chironomus			Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Midge Chironomus riparius		91		66		051		Reproduction ( of eggscase)			3387			766			591		591			Chironomus		Invert		Author reported
	Atlantic salmon Salmo salar		127		57		18		Biomass			6156			1112			1112		1112			Salmo		Fish		Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Brook trout Salvelinus fontinalis		123		655		19		Biomass			1644			59						Salvelinus			Author reported		wellRO mix
	Brook trout Salvelinus fontinalis		128		565		18		Biomass			1435			283			1293		1293			Salvelinus		Fish		Author reported		wellRO mix
	Fathead minnow Pimephales promelas		220		770		16		Biomass			6194			781						Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		well water
	Fathead minnow Pimephales promelas		96		620		025		Fry survival			4286			379			544		544			Pimephales		Fish		Author reported half the detection limit
	Zebrafish Danio rerio		83		61		025		Biomass			2344			241			241		241			Danio		Fish		Author reported half the detection limit
	Wood frog Rana sylvatica		115		469		16		MATC-survival		gt		2000		gt		2131		gt		2131		2131		gt		Rana		Amphib - Other Data		Value is from 2007 FW Cu AWQC Appendix C recommendation		aged tap water

	Appendix A Acceptable Acute Toxicity Data of Aluminum to Freshwater Aquatic Animals
	Species		Methoda		Hardness (mgL as CaCO3)		pH		DOC(mgL)			LC50 or EC50 Conc (microgL)		Reference			Normalized Conc - LC50(microgL)			Species Mean Acute Value(microgL)		Genus Mean Acute Value(microgL)			Genus			DOC Note		Dilution water
	Snail Physa sp		S M T		474		659		11		gt		23400		Call 1984 Call et al 1984		gt		4843			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		755		11			30600		Call 1984 Call et al 1984			2699			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		817		11		gt		24700		Call 1984 Call et al 1984		gt		2404			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		746		11			55500		Call 1984 Call et al 1984			5034			3546		3546			Physa		Mollusk		Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Fatmucket Lampsilis siliquoidea		F M T		1060		612		048		gt		6302		Wang et al 2016 Wang et al 2017		gt		2983		gt		2983		2983		gt		Lampsilis		Mollusk		Author reported
	Cladoceran Ceriodaphnia dubia		S M A		500		742		11			1900		McCauley et al 1986			173			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		S M A		505		786		11			1500		McCauley et al 1986			129			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		UW-S Lake Superior lab water
	Cladoceran Ceriodaphnia dubia		S M A		500		813		11			2560		McCauley et al 1986			243			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		R M T		26		75		05			720		ENSR 1992d			111			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		R M T		46		76		05			1880		ENSR 1992d			250			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		R M T		96		78		05			2450		ENSR 1992d			289			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		S U NR		90		715		05			3727		Fort and Stover 1995			500			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U NR		90		715		05			5673		Fort and Stover 1995			761			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U NR		89		76		05			2880		Soucek et al 2001			336			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		601		05			7112		European Al Association 2009			169			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		605		2			6865		European Al Association 2009			709			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		609		4			15581		European Al Association 2009			1007			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		601		05			681		European Al Association 2009			162			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		603		05			1630		European Al Association 2009			367			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		597		05			1785		European Al Association 2009			473			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		592		05			1410		European Al Association 2009			429			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		699		05		gt		1300		European Al Association 2009		gt		419			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		785		05		gt		5000		European Al Association 2009		gt		796			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		68		2		gt		10000		European Al Association 2009		gt		2076			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		782		2		gt		15000		European Al Association 2009		gt		1163			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		677		4		gt		10000		European Al Association 2009		gt		1516			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		766		4		gt		15000		European Al Association 2009		gt		861			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		79		05		gt		2000		European Al Association 2009		gt		315			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		789		05		gt		2000		European Al Association 2009		gt		316			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		604		05			1108		European Al Association 2009			85			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		598		2			11371		European Al Association 2009			482			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		573		4			80467		European Al Association 2009			4357			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		671		05		gt		10000		European Al Association 2009		gt		2379			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		783		05		gt		5000		European Al Association 2009		gt		634			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		679		2		gt		10000		European Al Association 2009		gt		1039			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		767		2		gt		15000		European Al Association 2009		gt		908			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		668		4		gt		15000		European Al Association 2009		gt		1246			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		762		4		gt		15000		European Al Association 2009		gt		633			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		606		2			33868		European Al Association 2009			792			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		56		4			104842		European Al Association 2009			4880			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		693		05		gt		5000		European Al Association 2009		gt		713			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		120		788		05		gt		5000		European Al Association 2009		gt		590			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		120		676		2		gt		15000		European Al Association 2009		gt		1212			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		771		2		gt		15000		European Al Association 2009		gt		810			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		66		4		gt		15000		European Al Association 2009		gt		1014			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		76		4		gt		15000		European Al Association 2009		gt		555			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			11971		European Al Association 2010			270			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			27478		European Al Association 2010			619			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			11998		European Al Association 2010			270			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		607		05			92495		European Al Association 2010			188			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		609		05			31337		European Al Association 2010			604			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		61		05			33235		European Al Association 2010			624			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		708		05		gt		8864		European Al Association 2010		gt		253			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		779		05		gt		42783		European Al Association 2010		gt		693			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		753		05			13204		European Al Association 2010			24			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		600		601		05			46326		European Al Association 2010			380			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		600		599		05		gt		8590		European Al Association 2010		gt		737			509				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia reticulata		F M T		451		60		11			304		Shephard 1983			201			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia reticulata		F M T		40		55		11			362		Shephard 1983			5531			1054		733			Ceriodaphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake SuperiorDI Mix
	Cladoceran Daphnia magna		S U NR		453		774		11			3900		Biesinger and Christensen 1972			338			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Daphnia magna		S M T		220		76		16			38200		Kimball 1978			2030			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Well water
	Cladoceran Daphnia magna		S U T		451		725		11			2800		Shephard 1983			287			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Daphnia magna		S U T		168		599		05			5000		European Al Association 2009		gt		227			-				Daphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Daphnia magna		S U T		168		792		05			7950		European Al Association 2009			92			333		333			Daphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Ostracod Stenocypris major		R M T		1563		651		16			310196		Shuhaimi-Othman et al 2011a			1022			1022		1022			Stenocypris		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (Malaysia)
	Amphipod Crangonyx pseudogracilis		R U T		50		675		16			9190		Martin and Holdich 1986			1217			1217		1217			Crangonyx		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (UK)
	Amphipod Hyalella azteca		F M T		105		613		048		gt		5997		Wang et al 2016 Wang et al 2017		gt		2800		gt		2800		2800		gt		Hyalella		Invert		Author reported
	Stonefly Acroneuria sp		S M T		474		746		11		gt		22600		Call et al 1984		gt		2050		gt		2050		2050		gt		Acroneuria		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Midge Chironomus plumosus		S U T		80		70		16			30000		Fargasova 2001			2536			2536		2536			Chironomus		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (Slovakia)
	Midge Paratanytarsus dissimilis		S M T		1743		728		28		gt		77700		Lamb and Bailey 1981 1983		gt		6232		gt		6232		6232		gt		Paratanytarsus		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Liberty Lake Washington
	Rainbow trout Oncorhynchus mykiss		F M T		274		758		05		gt		9840		Gundersen et al 1994		gt		1256			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		452		762		05		gt		8070		Gundersen et al 1994		gt		1140			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		895		758		05		gt		8160		Gundersen et al 1994		gt		1489			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		1304		758		05		gt		8200		Gundersen et al 1994		gt		1676			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		232		825		05			6170		Gundersen et al 1994			260			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		350		826		05			6170		Gundersen et al 1994			333			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		836		829		05			7670		Gundersen et al 1994			701			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		1158		830		05			6930		Gundersen et al 1994			777			801		801			Oncorhynchus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Atlantic salmon Salmo salar		S U T		68		55		05			584		Hamilton and Haines 1995			4602			-				Salmo			Value is from 2007 FW Cu AWQC Appendix C recommendation		Soft recon
	Atlantic salmon Salmo salar		S U T		68		65		05			599		Hamilton and Haines 1995			531			1564		1564			Salmo		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Soft recon
	Brook trout Salvelinus fontinalis		S U T		40		56		16			6530		Tandjung 1982			6947			-				Salvelinus			Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Brook trout Salvelinus fontinalis		S U T		18		56		16			3400		Tandjung 1982			6209			-				Salvelinus			Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Brook trout Salvelinus fontinalis		S U T		2		56		16			370		Tandjung 1982			2988			5051		5051			Salvelinus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Green sunfish Lepomis cyanellus		S M T		474		755		11		gt		50000		Call et al 1984		gt		5255		gt		5255		5255		gt		Lepomis		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Rio Grande silvery minnow Hybognathus amarus		R M T		140		81		05		gt		59100		Buhl 2002		gt		8620		gt		8620		8620		gt		Hybognathus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon water
	Fathead minnow Pimephales promelas		S M T		474		761		11		gt		48200		Call et al 1984		gt		4707			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Fathead minnow Pimephales promelas		S M T		474		805		11		gt		49800		Call et al 1984		gt		2837			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Fathead minnow Pimephales promelas		R M T		26		78		05			1160		ENSR 1992c			104			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		46		76		05			8180		ENSR 1992c			1191			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		96		81		05			20300		ENSR 1992c			2398			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		194		81		05			44800		ENSR 1992c			7839			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		140		81		05		gt		59100		Buhl 2002		gt		8620			2224		2224			Pimephales		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon water
	Smallmouth bass Micropterus dolomieui		S M T		1245		505		16			130		Kane and Rabeni 1987			859			859		859			Micropterus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Green tree frog Hyla cinerea		R M T		455		549		05		gt		4052		Jung and Jagoe 1995		gt		4377		gt		4377		4377		gt		Hyla		Amphibian		Value is from 2007 FW Cu AWQC Appendix C recommendation		artificial soft recon

	Normalization Chemistry
	pH		6					FAV		360
	Hardness		25					CMC		180
	DOC		1					CCC		99
	Acute Ranked GMAV											Chronic Ranked GMCV
	Rank			GMAV		Genus								Rank			GMCV		Genus
	18		gt		8620		Hybognathus		Fish							12		gt		2131		Rana		Amphib - Other Data
	17		gt		6232		Paratanytarsus		Invert							11			1710		Aeolosoma		Invert
	16		gt		5255		Lepomis		Fish							10			591		Chironomus		Invert
	15			5051		Salvelinus		Fish							9			544		Pimephales		Fish
	14		gt		4377		Hyla		Amphibian							8			488		Lymnaea		Mollusk
	13			3546		Physa		Mollusk							7			255		Brachionus		Invert
	12		gt		2983		Lampsilis		Mollusk							6			241		Danio		Fish
	11		gt		2800		Hyalella		Invert							5			171		Hyalella		Invert
	10			2536		Chironomus		Invert							4			129		Salvelinus		Fish
	9			2224		Pimephales		Fish							3			118		Ceriodaphnia		Invert
	8		gt		2050		Acroneuria		Invert							2			111		Salmo		Fish
	7			1564		Salmo		Fish							1			104		Lampsilis		Mollusk
	6			1217		Crangonyx		Invert
	5			1022		Stenocypris		Invert
	4			859		Micropterus		Fish
	3			801		Oncorhynchus		Fish
	2			733		Ceriodaphnia		Invert
	1			333		Daphnia		Invert
		N		Rank		Genus		GMAV		ln(GMAV)		ln(GMAV)2		P=R(N+1)		sqrt(P)				N		Rank		Genus		GMCV		ln(GMCV)		ln(GMCV)2		P=R(N+1)		sqrt(P)
		18		4		Micropterus		859		676		4564		0211		0459				12		4		Salvelinus		129		486		2364		0308		0555
			3		Oncorhynchus		801		669		4469		0158		0397					3		Ceriodaphnia		118		477		2277		0231		0480
			2		Ceriodaphnia		733		660		4352		0105		0324					2		Salmo		111		471		2220		0154		0392
			1		Daphnia		333		581		3374		0053		0229					1		Lampsilis		104		465		2159		0077		0277
			Sum				2585		1676		0526		141					Sum				1899		902		0769		170
			S2 =		1987										S2 =		059
			L =		4890										L =		4420
			A =		5887										A =		4592
			FAV =		360										FCV =		987
			CMC =		180

				All concentrations reported are microgL total Aluminum
				lt-----Click Button after entering data
	ENTER DATA HERE									ERRORNUM		ACUTE														CHRONIC
										Rank 1				Rank 2				Rank 3				Rank 4					Rank 1				Rank 2				Rank 3				Rank 4
	SiteName		DOC(mgL)		Hardness(mgL as CaCO3)		pH			FAV		CMC		CCC			Genus		GMAV			Genus		GMAV			Genus		GMAV			Genus		GMAV				Genus		GMCV			Genus		GMCV			Genus		GMCV			Genus		GMCV
	Site 1		50		25		9		75428737034162		35229324666008		1800		770			Daphnia			33452		Ceriodaphnia			73576		Stenocypris			102576		Crangonyx			122230			Lampsilis			10463		Ceriodaphnia			11865		Hyalella			17200		Brachionus			25648
	Site 2		50		50		9		75428737034162		31204991509063		1600		680			Daphnia			29631		Ceriodaphnia			65171		Stenocypris			90859		Crangonyx			108267			Lampsilis			9268		Ceriodaphnia			10510		Hyalella			15236		Brachionus			22718
	Site 3		50		75		9		75428737034162		29067527928199		1500		640			Daphnia			27601		Ceriodaphnia			60707		Stenocypris			84635		Crangonyx			100851			Lampsilis			8633		Ceriodaphnia			9790		Hyalella			14192		Brachionus			21162
	Site 4		50		100		9		75428737034162		27640367912592		1400		600			Daphnia			26246		Ceriodaphnia			57726		Stenocypris			80480		Crangonyx			95899			Lampsilis			8209		Ceriodaphnia			9309		Hyalella			13495		Brachionus			20123
	Site 5		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 6		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 7		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 8		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 9		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 10		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 11		50		25		85		75428737034162		63245275664384		3200		1400			Daphnia			60055		Ceriodaphnia			132086		Stenocypris			184149		Crangonyx			219432			Lampsilis			18783		Ceriodaphnia			21301		Hyalella			30879		Brachionus			46045
	Site 12		50		50		85		75428737034162		61388044609926		3100		1300			Daphnia			58291		Ceriodaphnia			128208		Stenocypris			178742		Crangonyx			212988			Lampsilis			18232		Ceriodaphnia			20676		Hyalella			29972		Brachionus			44693
	Site 13		50		75		85		75428737034162		60327020429132		3000		1300			Daphnia			57284		Ceriodaphnia			125992		Stenocypris			175652		Crangonyx			209307			Lampsilis			17917		Ceriodaphnia			20318		Hyalella			29454		Brachionus			43920
	Site 14		50		100		85		75428737034162		59585352130146		3000		1300			Daphnia			56580		Ceriodaphnia			124443		Stenocypris			173493		Crangonyx			206734			Lampsilis			17696		Ceriodaphnia			20068		Hyalella			29092		Salmo			41264
	Site 15		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 16		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 17		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 18		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 19		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 20		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370

	Appendix C Acceptable Chronic Toxicity Data of Aluminum to Freshwater Aquatic Animals
									MLR equation
	Species		Hardness (mgL as CaCO3)		pH		DOC(mgL)		EC20 Endpoint			EC20(microgL)			Normalized Conc- EC20(microgL)			SMCV-EC20(microgL)		GMCV-EC20(microgL)			Genus			DOC Notes		Dilution water
	Oligochaete Aeolosoma sp		48		6		025		Reproduction (population size)			1235			17098			17098		17098			Aeolosoma		Invert		Author reported half the detection limit
	Rotifer Brachionus calyciflorus		100		62		025		Reproduction (population size)			431			2555			2555		2555			Brachionus		Invert		Author reported half the detection limit
	Great pond snail Lymnaea stagnalis		117		61		025		Biomass			746			4877			4877		4877			Lymnaea		Mollusk		Author reported half the detection limit
	Fatmucket Lampsilis siliquoidea		1055		604		040		Biomass			169			1042			1042		1042			Lampsilis		Mollusk		Author reported
	Cladoceran Ceriodaphnia dubia		50		715		11		Reproduction - youngstarting adult			1780			1898						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		26		73		05		Reproduction - youngfemale			1557			2718						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		46		75		05		Reproduction - youngfemale			8087			1102						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		96		79		05		Reproduction - youngfemale			6519			796						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		194		81		05		Reproduction - youngfemale			6836			888						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		255		703		05		Reproduction (youngfemale)			250			563						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		122		713		05		Reproduction (youngfemale)			860			1061						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		25		798		05		Reproduction (youngfemale)			700			1006						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		61		803		05		Reproduction (youngfemale)			1010			1363						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		122		810		05		Reproduction (youngfemale)			870			1164						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		255		633		05		Reproduction (youngfemale)			260			1758						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		121		636		05		Reproduction (youngfemale)			390			1101			1182		1182			Ceriodaphnia		Invert		Author reported
	Amphipod Hyalella azteca		95		61		051		Biomass (28 d)			1993			1014						Hyalella			Author reported
	Amphipod Hyalella azteca		106		604		033		Biomass (28 d)			425			2895			1713		1713			Hyalella		Invert		Author reported
	Midge Chironomus riparius		118		558		18		Adult midge emergence			2955			1192						Chironomus			Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Midge Chironomus riparius		119		505		18		Adult midge emergence			8442			22578						Chironomus			Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Midge Chironomus riparius		91		66		051		Reproduction ( of eggscase)			3387			7664			5908		5908			Chironomus		Invert		Author reported
	Atlantic salmon Salmo salar		127		57		18		Biomass			6156			5085			5085		5085			Salmo		Fish		Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Brook trout Salvelinus fontinalis		123		655		19		Biomass			1644			270						Salvelinus			Author reported		wellRO mix
	Brook trout Salvelinus fontinalis		128		565		18		Biomass			1435			1295			5914		5914			Salvelinus		Fish		Author reported		wellRO mix
	Fathead minnow Pimephales promelas		220		770		16		Biomass			6194			3569						Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		well water
	Fathead minnow Pimephales promelas		96		620		025		Fry survival			4286			1734			2488		2488			Pimephales		Fish		Author reported half the detection limit
	Zebrafish Danio rerio		83		61		025		Biomass			2344			1102			1102		1102			Danio		Fish		Author reported half the detection limit
	Wood frog Rana sylvatica		115		469		16		MATC-survival		gt		2000		gt		9746		gt		9746		9746		gt		Rana		Amphib - Other Data		Value is from 2007 FW Cu AWQC Appendix C recommendation		aged tap water

	Appendix A Acceptable Acute Toxicity Data of Aluminum to Freshwater Aquatic Animals
	Species		Methoda		Hardness (mgL as CaCO3)		pH		DOC(mgL)			LC50 or EC50 Conc (microgL)		Reference			Normalized Conc - LC50(microgL)			Species Mean Acute Value(microgL)		Genus Mean Acute Value(microgL)			Genus			DOC Note		Dilution water
	Snail Physa sp		S M T		474		659		11		gt		23400		Call 1984 Call et al 1984		gt		48425			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		755		11			30600		Call 1984 Call et al 1984			26986			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		817		11		gt		24700		Call 1984 Call et al 1984		gt		24040			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		746		11			55500		Call 1984 Call et al 1984			50338			35462		35462			Physa		Mollusk		Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Fatmucket Lampsilis siliquoidea		F M T		1060		612		048		gt		6302		Wang et al 2016 Wang et al 2017		gt		29834		gt		29834		29834		gt		Lampsilis		Mollusk		Author reported
	Cladoceran Ceriodaphnia dubia		S M A		500		742		11			1900		McCauley et al 1986			1728			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		S M A		505		786		11			1500		McCauley et al 1986			1294			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		UW-S Lake Superior lab water
	Cladoceran Ceriodaphnia dubia		S M A		500		813		11			2560		McCauley et al 1986			2431			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		R M T		26		75		05			720		ENSR 1992d			1113			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		R M T		46		76		05			1880		ENSR 1992d			2495			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		R M T		96		78		05			2450		ENSR 1992d			2894			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		S U NR		90		715		05			3727		Fort and Stover 1995			5002			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U NR		90		715		05			5673		Fort and Stover 1995			7613			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U NR		89		76		05			2880		Soucek et al 2001			3362			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		601		05			7112		European Al Association 2009			1691			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		605		2			6865		European Al Association 2009			7088			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		609		4			15581		European Al Association 2009			10072			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		601		05			681		European Al Association 2009			1620			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		603		05			1630		European Al Association 2009			3674			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		597		05			1785		European Al Association 2009			4732			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		592		05			1410		European Al Association 2009			4295			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		699		05		gt		1300		European Al Association 2009		gt		4189			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		785		05		gt		5000		European Al Association 2009		gt		7960			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		68		2		gt		10000		European Al Association 2009		gt		20755			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		782		2		gt		15000		European Al Association 2009		gt		11626			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		677		4		gt		10000		European Al Association 2009		gt		15161			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		766		4		gt		15000		European Al Association 2009		gt		8608			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		79		05		gt		2000		European Al Association 2009		gt		3150			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		789		05		gt		2000		European Al Association 2009		gt		3156			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		604		05			1108		European Al Association 2009			850			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		598		2			11371		European Al Association 2009			4817			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		573		4			80467		European Al Association 2009			43570			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		671		05		gt		10000		European Al Association 2009		gt		23786			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		783		05		gt		5000		European Al Association 2009		gt		6344			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		679		2		gt		10000		European Al Association 2009		gt		10394			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		767		2		gt		15000		European Al Association 2009		gt		9082			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		668		4		gt		15000		European Al Association 2009		gt		12461			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		762		4		gt		15000		European Al Association 2009		gt		6332			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		606		2			33868		European Al Association 2009			7920			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		56		4			104842		European Al Association 2009			48800			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		693		05		gt		5000		European Al Association 2009		gt		7126			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		120		788		05		gt		5000		European Al Association 2009		gt		5898			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		120		676		2		gt		15000		European Al Association 2009		gt		12117			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		771		2		gt		15000		European Al Association 2009		gt		8096			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		66		4		gt		15000		European Al Association 2009		gt		10139			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		76		4		gt		15000		European Al Association 2009		gt		5545			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			11971		European Al Association 2010			2699			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			27478		European Al Association 2010			6194			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			11998		European Al Association 2010			2705			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		607		05			92495		European Al Association 2010			1876			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		609		05			31337		European Al Association 2010			6035			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		61		05			33235		European Al Association 2010			6238			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		708		05		gt		8864		European Al Association 2010		gt		2534			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		779		05		gt		42783		European Al Association 2010		gt		6930			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		753		05			13204		European Al Association 2010			244			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		600		601		05			46326		European Al Association 2010			3798			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		600		599		05		gt		8590		European Al Association 2010		gt		7366			5094				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia reticulata		F M T		451		60		11			304		Shephard 1983			2009			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia reticulata		F M T		40		55		11			362		Shephard 1983			55307			10542		7328			Ceriodaphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake SuperiorDI Mix
	Cladoceran Daphnia magna		S U NR		453		774		11			3900		Biesinger and Christensen 1972			3382			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Daphnia magna		S M T		220		76		16			38200		Kimball 1978			20303			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Well water
	Cladoceran Daphnia magna		S U T		451		725		11			2800		Shephard 1983			2868			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Daphnia magna		S U T		168		599		05			5000		European Al Association 2009		gt		2265			-				Daphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Daphnia magna		S U T		168		792		05			7950		European Al Association 2009			920			3332		3332			Daphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Ostracod Stenocypris major		R M T		1563		651		16			310196		Shuhaimi-Othman et al 2011a			10216			10216		10216			Stenocypris		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (Malaysia)
	Amphipod Crangonyx pseudogracilis		R U T		50		675		16			9190		Martin and Holdich 1986			12174			12174		12174			Crangonyx		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (UK)
	Amphipod Hyalella azteca		F M T		105		613		048		gt		5997		Wang et al 2016 Wang et al 2017		gt		28002		gt		28002		28002		gt		Hyalella		Invert		Author reported
	Stonefly Acroneuria sp		S M T		474		746		11		gt		22600		Call et al 1984		gt		20498		gt		20498		20498		gt		Acroneuria		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Midge Chironomus plumosus		S U T		80		70		16			30000		Fargasova 2001			25361			25361		25361			Chironomus		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (Slovakia)
	Midge Paratanytarsus dissimilis		S M T		1743		728		28		gt		77700		Lamb and Bailey 1981 1983		gt		62318		gt		62318		62318		gt		Paratanytarsus		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Liberty Lake Washington
	Rainbow trout Oncorhynchus mykiss		F M T		274		758		05		gt		9840		Gundersen et al 1994		gt		5745			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		452		762		05		gt		8070		Gundersen et al 1994		gt		5212			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		895		758		05		gt		8160		Gundersen et al 1994		gt		6807			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		1304		758		05		gt		8200		Gundersen et al 1994		gt		7662			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		232		825		05			6170		Gundersen et al 1994			1190			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		350		826		05			6170		Gundersen et al 1994			1523			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		836		829		05			7670		Gundersen et al 1994			3205			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		1158		830		05			6930		Gundersen et al 1994			3554			3661		3661			Oncorhynchus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Atlantic salmon Salmo salar		S U T		68		55		05			584		Hamilton and Haines 1995			21042			-				Salmo			Value is from 2007 FW Cu AWQC Appendix C recommendation		Soft recon
	Atlantic salmon Salmo salar		S U T		68		65		05			599		Hamilton and Haines 1995			2430			7151		7151			Salmo		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Soft recon
	Brook trout Salvelinus fontinalis		S U T		40		56		16			6530		Tandjung 1982			31767			-				Salvelinus			Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Brook trout Salvelinus fontinalis		S U T		18		56		16			3400		Tandjung 1982			28391			-				Salvelinus			Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Brook trout Salvelinus fontinalis		S U T		2		56		16			370		Tandjung 1982			13663			23097		23097			Salvelinus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Green sunfish Lepomis cyanellus		S M T		474		755		11		gt		50000		Call et al 1984		gt		24028		gt		24028		24028		gt		Lepomis		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Rio Grande silvery minnow Hybognathus amarus		R M T		140		81		05		gt		59100		Buhl 2002		gt		39414		gt		39414		39414		gt		Hybognathus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon water
	Fathead minnow Pimephales promelas		S M T		474		761		11		gt		48200		Call et al 1984		gt		21522			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Fathead minnow Pimephales promelas		S M T		474		805		11		gt		49800		Call et al 1984		gt		12972			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Fathead minnow Pimephales promelas		R M T		26		78		05			1160		ENSR 1992c			477			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		46		76		05			8180		ENSR 1992c			5446			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		96		81		05			20300		ENSR 1992c			10966			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		194		81		05			44800		ENSR 1992c			35847			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		140		81		05		gt		59100		Buhl 2002		gt		39414			10168		10168			Pimephales		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon water
	Smallmouth bass Micropterus dolomieui		S M T		1245		505		16			130		Kane and Rabeni 1987			3929			3929		3929			Micropterus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Green tree frog Hyla cinerea		R M T		455		549		05		gt		4052		Jung and Jagoe 1995		gt		20016		gt		20016		20016		gt		Hyla		Amphibian		Value is from 2007 FW Cu AWQC Appendix C recommendation		artificial soft recon

	Normalization Chemistry
	pH		7					FAV		2741
	Hardness		100					CMC		1400
	DOC		1					CCC		390
	Acute Ranked GMAV											Chronic Ranked GMCV
	Rank			GMAV		Genus								Rank			GMCV		Genus
	18		gt		62318		Paratanytarsus		Invert							12			17098		Aeolosoma		Invert
	17		gt		39414		Hybognathus		Fish							11		gt		9746		Rana		Amphib - Other Data
	16			35462		Physa		Mollusk							10			5908		Chironomus		Invert
	15		gt		29834		Lampsilis		Mollusk							9			4877		Lymnaea		Mollusk
	14		gt		28002		Hyalella		Invert							8			2555		Brachionus		Invert
	13			25361		Chironomus		Invert							7			2488		Pimephales		Fish
	12		gt		24028		Lepomis		Fish							6			1713		Hyalella		Invert
	11			23097		Salvelinus		Fish							5			1182		Ceriodaphnia		Invert
	10		gt		20498		Acroneuria		Invert							4			1102		Danio		Fish
	9		gt		20016		Hyla		Amphibian							3			1042		Lampsilis		Mollusk
	8			12174		Crangonyx		Invert							2			591		Salvelinus		Fish
	7			10216		Stenocypris		Invert							1			509		Salmo		Fish
	6			10168		Pimephales		Fish
	5			7328		Ceriodaphnia		Invert
	4			7151		Salmo		Fish
	3			3929		Micropterus		Fish
	2			3661		Oncorhynchus		Fish
	1			3332		Daphnia		Invert
		N		Rank		Genus		GMAV		ln(GMAV)		ln(GMAV)2		P=R(N+1)		sqrt(P)				N		Rank		Genus		GMCV		ln(GMCV)		ln(GMCV)2		P=R(N+1)		sqrt(P)
		18		4		Salmo		7151		887		7876		0211		0459				12		4		Danio		1102		700		4907		0308		0555
			3		Micropterus		3929		828		6849		0158		0397					3		Lampsilis		1042		695		4829		0231		0480
			2		Oncorhynchus		3661		821		6733		0105		0324					2		Salvelinus		591		638		4074		0154		0392
			1		Daphnia		3332		811		6579		0053		0229					1		Salmo		509		623		3883		0077		0277
			Sum				3347		2804		0526		141					Sum				2657		1769		0769		170
			S2 =		1223										S2 =		1080
			L =		7134										L =		5242
			A =		7916										A =		5976
			FAV =		2741										FCV =		3940
			CMC =		1371

				All concentrations reported are microgL total Aluminum
				lt-----Click Button after entering data
	ENTER DATA HERE									ERRORNUM		ACUTE														CHRONIC
										Rank 1				Rank 2				Rank 3				Rank 4					Rank 1				Rank 2				Rank 3				Rank 4
	SiteName		DOC(mgL)		Hardness(mgL as CaCO3)		pH			FAV		CMC		CCC			Genus		GMAV			Genus		GMAV			Genus		GMAV			Genus		GMAV				Genus		GMCV			Genus		GMCV			Genus		GMCV			Genus		GMCV
	Site 1		50		25		9		75428737034162		35229324666008		1800		770			Daphnia			33452		Ceriodaphnia			73576		Stenocypris			102576		Crangonyx			122230			Lampsilis			10463		Ceriodaphnia			11865		Hyalella			17200		Brachionus			25648
	Site 2		50		50		9		75428737034162		31204991509063		1600		680			Daphnia			29631		Ceriodaphnia			65171		Stenocypris			90859		Crangonyx			108267			Lampsilis			9268		Ceriodaphnia			10510		Hyalella			15236		Brachionus			22718
	Site 3		50		75		9		75428737034162		29067527928199		1500		640			Daphnia			27601		Ceriodaphnia			60707		Stenocypris			84635		Crangonyx			100851			Lampsilis			8633		Ceriodaphnia			9790		Hyalella			14192		Brachionus			21162
	Site 4		50		100		9		75428737034162		27640367912592		1400		600			Daphnia			26246		Ceriodaphnia			57726		Stenocypris			80480		Crangonyx			95899			Lampsilis			8209		Ceriodaphnia			9309		Hyalella			13495		Brachionus			20123
	Site 5		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 6		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 7		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 8		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 9		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 10		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 11		50		25		85		75428737034162		63245275664384		3200		1400			Daphnia			60055		Ceriodaphnia			132086		Stenocypris			184149		Crangonyx			219432			Lampsilis			18783		Ceriodaphnia			21301		Hyalella			30879		Brachionus			46045
	Site 12		50		50		85		75428737034162		61388044609926		3100		1300			Daphnia			58291		Ceriodaphnia			128208		Stenocypris			178742		Crangonyx			212988			Lampsilis			18232		Ceriodaphnia			20676		Hyalella			29972		Brachionus			44693
	Site 13		50		75		85		75428737034162		60327020429132		3000		1300			Daphnia			57284		Ceriodaphnia			125992		Stenocypris			175652		Crangonyx			209307			Lampsilis			17917		Ceriodaphnia			20318		Hyalella			29454		Brachionus			43920
	Site 14		50		100		85		75428737034162		59585352130146		3000		1300			Daphnia			56580		Ceriodaphnia			124443		Stenocypris			173493		Crangonyx			206734			Lampsilis			17696		Ceriodaphnia			20068		Hyalella			29092		Salmo			41264
	Site 15		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 16		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 17		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 18		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 19		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 20		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370

EstuarineMarine Minimum Data Requirements

15

Family Minimum Data Requirement EstuarineMarine

Acute(Phylum Family Genus)

Chronic(Phylum Family Genus)

Family in the phylum Chordata - -

Family in the phylum Chordata - -

Either the Mysidae or Penaeidae family - -

Family in a phylum other than Arthropoda or Chordata Mollusca Ostreidae Crassostrea -

Family in a phylum other than Chordata Annelida Nereididae Neanthes -

Family in a phylum other than Chordata Annelida Capitellidae Capitella -

Family in a phylum other than Chordata Annelida Ctenodrilidae Ctenodrilus -

Any other family Arthropoda Ameiridae Nitokra -

II Evaluate water quality conditions to determine applicability

bull Criteria are often based on one or more water quality conditions (eg hardness) that affect toxicity of the material to test organisms

bull Aluminum toxicity is highly dependent on the pH of the test waterndash The pH of test waters affects the amount and form

of aluminum that is bioavailable to test speciesndash Hardness and DOC (Dissolved Organic Carbon)

also influence the amount of aluminum that is bioavailable

16

Multiple Bioavailability Modeling Approaches Underwent External Peer Review

17

bull Biotic Ligand Modelsbull Full aluminum BLM (2016 model version) ndash Temperature pH

Dissolved organic carbon (DOC) Major cations (calcium [Ca] magnesium [Mg] sodium [Na] and potassium [K]) Major anions (sulfate [SO4] and chloride [Cl]) Alkalinity and Sulfide

bull Simplified aluminum BLM - pH hardness DOC temperature

bull Multilinear Regression Equationsbull pH and Hardness

Accounting for Effects of Water Chemistry on Bioavailability and Toxicity

bull Water quality criteria can be modified quantitatively when enough data are available to demonstrate that those conditions similarly affect the results with a variety of speciesndash High quality data in the draft aluminum document demonstrated

that pH DOC and hardness affected a variety of species in a similar way when exposed to aluminum concentrations

bull Recent publications by Cardwell et al (2017) and Gensemer et al (2017) summarized short-term aluminum chronic toxicity data across a range of DOC pH and hardness values for three species

18

Multiple Linear Regression (MLR) Models to Account for Effects of Water Chemistry

bull MLR models were developed for each species (DeForest et al 2017)ndash 119862119862 119889119889119906119906119887119887119894119894119886119886 11986411986411986211986220=119890119890[minus41026+[0525timesln(119863119863119874119874119862119862)]+[2201timesln(ℎ119886119886119903119903119889119889)]+(11282times119901119901119867119867)minus(0633times1199011199011198671198672)minus[0264times119901119901119867119867ln(ℎ119886119886119903119903119889119889)]]

ndash 119875119875 119901119901119903119903119900119900119898119898119890119890119897119897119886119886119904119904 11986411986411986211986220=119890119890[minus14029+[0503timesln(119863119863119874119874119862119862)]+[3443timesln(ℎ119886119886119903119903119889119889)]+(3131times119901119901119867119867)minus[0494times119901119901119867119867ln(ℎ119886119886119903119903119889119889)]]

bull These models were applied to both the acute and chronic aluminum toxicity datandash The C dubia model was applied to invertebrate datandash The P promelas model was applied to vertebrate data

19

III Normalize toxicity data in species sensitivity distribution to a set of water

quality conditionsbull Just like single parameter hardness models the MLR

models were used to normalize the aluminum toxicity data to a single set of water quality conditionsndash As an illustration for one set of calculations the draft

aluminum AWQC set these conditions to pH 7 hardness of 100 mgL and DOC of 1 mgL

bull This allows for comparisons to be made between species based on their sensitivity to aluminumndash Apples to apples

20

IV Construct sensitivity distribution to determine criteria at set conditions

21

Figure 8 Ranked Summary of Total Aluminum Genus Mean Acute Values (GMAVs) - Freshwater at pH 7 Hardness of 100 mgL and DOC of 1 mgL

IV Construct sensitivity distribution to determine criteria at set conditions

22

Figure 10 Ranked Summary of Total Aluminum Genus Mean Chronic Values (GMCVs) ndash Freshwater Supplemented with Other Data to Fulfill Missing MDRs at pH 7 Hardness of 100 mgL and DOC of 1 mgL

Aluminum Criteria Calculatorbull The relative ranking of genera in the sensitivity

distribution to which the toxicity data are normalized will change based on the set of water chemistry conditions (ie pH hardness and DOC)

bull To make calculating aluminum criteria easier EPA developed an Aluminum Criteria Calculatorndash Automated so that user only needs to enter water chemistry

conditions of interestndash Allows quick calculations for a variety of conditionsndash Limits calculations to the recommended conditions for which

the MLR models were developed were specified for hardness and DOC

bull No extrapolation beyond hardness and DOC empirical data bounds

23

Aluminum Criteria Calculator SD Example

24

pH=7 Hardness=100 DOC=1 pH=6 Hardness=25 DOC=1

Chronic Ranked GMCVRank GMCV Genus

12 17098 Aeolosoma Invert11 gt 9746 Rana Amphib - Other Data10 5908 Chironomus Invert

9 4877 Lymnaea Mollusk8 2555 Brachionus Invert7 2488 Pimephales Fish6 1713 Hyalella Invert5 1182 Ceriodaphnia Invert4 1102 Danio Fish3 1042 Lampsilis Mollusk2 591 Salvelinus Fish1 509 Salmo Fish

FCV = 3940 microgL total aluminum

Chronic Ranked GMCVRank GMCV Genus

12 gt 2131 Rana Amphib - Other Data11 1710 Aeolosoma Invert10 591 Chironomus Invert

9 544 Pimephales Fish8 488 Lymnaea Mollusk7 255 Brachionus Invert6 241 Danio Fish5 171 Hyalella Invert4 129 Salvelinus Fish3 118 Ceriodaphnia Invert2 111 Salmo Fish1 104 Lampsilis Mollusk

FCV = 987 microgL total aluminum

Multiple Scenarios

Run Scenarios

Summary Sheet

Acute Dataset

Chronic Dataset

Multiple Scenarios

Run Scenarios

Summary Sheet

Acute Dataset

Chronic Dataset

Boundaries of the Criteria Outputsbull MLR models were developed using data that

encompassndash DOC range of 008-5 mgLndash Hardness range of 98-127 mgL (as CaCO3)ndash pH range of 6-81

bull MLR criteria outputs are bounded atndash Maximum of 150 mgL total hardness (as CaCO3)

ndash DOC of 50 mgLndash pH range of the model is 50 to 90

25

Aluminum Criteria Look-Up Tables

26

bull EPA also provided criteria look-up tables in Appendix K

Har

dnes

s

CCC(microgL total aluminum)

(DOC=05 mgL)

pH 50 pH 55 pH 60 pH 65 pH 70 pH 75 pH 80 pH 85 pH 90

25 23 a 14 b 69 c 160 f 350 f 620 i 540 b 410 a 230 a

50 43 a 24 b 100 d 170 f 310 f 630 f 670 j 400 a 200 a

75 61 a 33 b 130 e 180 f 290 f 530 f 730 i 390 a 190 a

100 78 a 41 b 150 e 190 f 280 g 460 f 770 c 390 a 180 a

150 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

200 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

250 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

300 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

350 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

400 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

Aluminum Criteria Look-Up Tables

27

bull EPA also provided criteria look-up tables in Appendix K

Har

dnes

s

CCC(microgL total aluminum)

(DOC=10 mgL)

pH 50 pH 55 pH 60 pH 65 pH 70 pH 75 pH 80 pH 85 pH 90

25 33 a 21 b 99 c 220 f 490 f 900 i 780 b 590 a 330 a

50 61 a 35 b 150 e 250 f 440 f 890 f 960 j 580 a 290 a

75 88 a 47 b 180 e 260 f 410 f 740 f 1100 i 570 a 270 a

100 11 a 59 b 210 e 270 f 390 g 650 f 1100 c 560 b 260 a

150 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

200 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

250 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

300 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

350 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

400 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

Aluminum Criteria Look-Up Tables

28

bull EPA also provided criteria look-up tables in Appendix K

Har

dnes

s

CCC(microgL total aluminum)

(DOC=25 mgL)

pH 50 pH 55 pH 60 pH 65 pH 70 pH 75 pH 80 pH 85 pH 90

25 54 a 34 b 160 c 350 f 770 f 1500 i 1300 b 960 a 540 a

50 99 a 57 b 230 e 390 f 690 f 1400 f 1600 j 930 a 470 a

75 14 a 77 b 290 e 410 f 640 f 1200 f 1700 i 920 a 440 a

100 18 a 96 b 330 e 420 f 620 g 1000 f 1800 d 920 b 420 a

150 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

200 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

250 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

300 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

350 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

400 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

Aluminum Criteria Look-Up Tables

29

bull EPA also provided criteria look-up tables in Appendix K

Har

dnes

s

CCC(microgL total aluminum)

(DOC=50 mgL)

pH 50 pH 55 pH 60 pH 65 pH 70 pH 75 pH 80 pH 85 pH 90

25 77 a 49 b 230 c 500 f 1100 f 2100 c 1900 b 1400 a 770 a

50 14 a 82 b 330 e 540 f 970 f 2000 f 2200 j 1300 a 680 a

75 20 a 110 b 410 e 570 f 900 f 1600 f 2500 i 1300 a 640 a

100 26 a 140 b 460 f 590 f 880 g 1400 f 2500 d 1300 b 600 a

150 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

200 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

250 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

300 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

350 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

400 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

Status

30

bull Draft Document Released to the Federal Register on 72817ndash Public comment period of 60 days ndash Due Date for Comments is 92617

bull Revisions will be made considering public commentsbull Subsequent EPA intra-agency review of final criteria

document will then be conducted bull Publication of final criteria document planned for Late Fall

2017

Federal Register Webpagehttpswwwfederalregistergovdocuments201

707282017-15968request-for-scientific-views-draft-updated-aquatic-life-ambient-water-quality-criteria-for-aluminum

Aluminum Criteria Webpagehttpswwwepagovwqc2017-draft-aquatic-life-

criteria-aluminum-freshwater-documents

31

	Appendix C Acceptable Chronic Toxicity Data of Aluminum to Freshwater Aquatic Animals
									MLR equation
	Species		Hardness (mgL as CaCO3)		pH		DOC(mgL)		EC20 Endpoint			EC20(microgL)			Normalized Conc- EC20(microgL)			SMCV-EC20(microgL)		GMCV-EC20(microgL)			Genus			DOC Notes		Dilution water
	Oligochaete Aeolosoma sp		48		6		025		Reproduction (population size)			1235			1710			1710		1710			Aeolosoma		Invert		Author reported half the detection limit
	Rotifer Brachionus calyciflorus		100		62		025		Reproduction (population size)			431			255			255		255			Brachionus		Invert		Author reported half the detection limit
	Great pond snail Lymnaea stagnalis		117		61		025		Biomass			746			488			488		488			Lymnaea		Mollusk		Author reported half the detection limit
	Fatmucket Lampsilis siliquoidea		1055		604		040		Biomass			169			104			104		104			Lampsilis		Mollusk		Author reported
	Cladoceran Ceriodaphnia dubia		50		715		11		Reproduction - youngstarting adult			1780			190						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		26		73		05		Reproduction - youngfemale			1557			272						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		46		75		05		Reproduction - youngfemale			8087			110						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		96		79		05		Reproduction - youngfemale			6519			80						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		194		81		05		Reproduction - youngfemale			6836			89						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		255		703		05		Reproduction (youngfemale)			250			56						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		122		713		05		Reproduction (youngfemale)			860			106						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		25		798		05		Reproduction (youngfemale)			700			101						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		61		803		05		Reproduction (youngfemale)			1010			136						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		122		810		05		Reproduction (youngfemale)			870			116						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		255		633		05		Reproduction (youngfemale)			260			176						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		121		636		05		Reproduction (youngfemale)			390			110			118		118			Ceriodaphnia		Invert		Author reported
	Amphipod Hyalella azteca		95		61		051		Biomass (28 d)			1993			101						Hyalella			Author reported
	Amphipod Hyalella azteca		106		604		033		Biomass (28 d)			425			289			171		171			Hyalella		Invert		Author reported
	Midge Chironomus riparius		118		558		18		Adult midge emergence			2955			119						Chironomus			Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Midge Chironomus riparius		119		505		18		Adult midge emergence			8442			2258						Chironomus			Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Midge Chironomus riparius		91		66		051		Reproduction ( of eggscase)			3387			766			591		591			Chironomus		Invert		Author reported
	Atlantic salmon Salmo salar		127		57		18		Biomass			6156			1112			1112		1112			Salmo		Fish		Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Brook trout Salvelinus fontinalis		123		655		19		Biomass			1644			59						Salvelinus			Author reported		wellRO mix
	Brook trout Salvelinus fontinalis		128		565		18		Biomass			1435			283			1293		1293			Salvelinus		Fish		Author reported		wellRO mix
	Fathead minnow Pimephales promelas		220		770		16		Biomass			6194			781						Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		well water
	Fathead minnow Pimephales promelas		96		620		025		Fry survival			4286			379			544		544			Pimephales		Fish		Author reported half the detection limit
	Zebrafish Danio rerio		83		61		025		Biomass			2344			241			241		241			Danio		Fish		Author reported half the detection limit
	Wood frog Rana sylvatica		115		469		16		MATC-survival		gt		2000		gt		2131		gt		2131		2131		gt		Rana		Amphib - Other Data		Value is from 2007 FW Cu AWQC Appendix C recommendation		aged tap water

	Appendix A Acceptable Acute Toxicity Data of Aluminum to Freshwater Aquatic Animals
	Species		Methoda		Hardness (mgL as CaCO3)		pH		DOC(mgL)			LC50 or EC50 Conc (microgL)		Reference			Normalized Conc - LC50(microgL)			Species Mean Acute Value(microgL)		Genus Mean Acute Value(microgL)			Genus			DOC Note		Dilution water
	Snail Physa sp		S M T		474		659		11		gt		23400		Call 1984 Call et al 1984		gt		4843			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		755		11			30600		Call 1984 Call et al 1984			2699			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		817		11		gt		24700		Call 1984 Call et al 1984		gt		2404			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		746		11			55500		Call 1984 Call et al 1984			5034			3546		3546			Physa		Mollusk		Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Fatmucket Lampsilis siliquoidea		F M T		1060		612		048		gt		6302		Wang et al 2016 Wang et al 2017		gt		2983		gt		2983		2983		gt		Lampsilis		Mollusk		Author reported
	Cladoceran Ceriodaphnia dubia		S M A		500		742		11			1900		McCauley et al 1986			173			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		S M A		505		786		11			1500		McCauley et al 1986			129			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		UW-S Lake Superior lab water
	Cladoceran Ceriodaphnia dubia		S M A		500		813		11			2560		McCauley et al 1986			243			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		R M T		26		75		05			720		ENSR 1992d			111			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		R M T		46		76		05			1880		ENSR 1992d			250			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		R M T		96		78		05			2450		ENSR 1992d			289			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		S U NR		90		715		05			3727		Fort and Stover 1995			500			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U NR		90		715		05			5673		Fort and Stover 1995			761			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U NR		89		76		05			2880		Soucek et al 2001			336			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		601		05			7112		European Al Association 2009			169			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		605		2			6865		European Al Association 2009			709			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		609		4			15581		European Al Association 2009			1007			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		601		05			681		European Al Association 2009			162			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		603		05			1630		European Al Association 2009			367			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		597		05			1785		European Al Association 2009			473			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		592		05			1410		European Al Association 2009			429			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		699		05		gt		1300		European Al Association 2009		gt		419			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		785		05		gt		5000		European Al Association 2009		gt		796			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		68		2		gt		10000		European Al Association 2009		gt		2076			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		782		2		gt		15000		European Al Association 2009		gt		1163			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		677		4		gt		10000		European Al Association 2009		gt		1516			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		766		4		gt		15000		European Al Association 2009		gt		861			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		79		05		gt		2000		European Al Association 2009		gt		315			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		789		05		gt		2000		European Al Association 2009		gt		316			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		604		05			1108		European Al Association 2009			85			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		598		2			11371		European Al Association 2009			482			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		573		4			80467		European Al Association 2009			4357			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		671		05		gt		10000		European Al Association 2009		gt		2379			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		783		05		gt		5000		European Al Association 2009		gt		634			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		679		2		gt		10000		European Al Association 2009		gt		1039			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		767		2		gt		15000		European Al Association 2009		gt		908			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		668		4		gt		15000		European Al Association 2009		gt		1246			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		762		4		gt		15000		European Al Association 2009		gt		633			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		606		2			33868		European Al Association 2009			792			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		56		4			104842		European Al Association 2009			4880			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		693		05		gt		5000		European Al Association 2009		gt		713			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		120		788		05		gt		5000		European Al Association 2009		gt		590			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		120		676		2		gt		15000		European Al Association 2009		gt		1212			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		771		2		gt		15000		European Al Association 2009		gt		810			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		66		4		gt		15000		European Al Association 2009		gt		1014			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		76		4		gt		15000		European Al Association 2009		gt		555			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			11971		European Al Association 2010			270			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			27478		European Al Association 2010			619			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			11998		European Al Association 2010			270			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		607		05			92495		European Al Association 2010			188			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		609		05			31337		European Al Association 2010			604			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		61		05			33235		European Al Association 2010			624			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		708		05		gt		8864		European Al Association 2010		gt		253			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		779		05		gt		42783		European Al Association 2010		gt		693			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		753		05			13204		European Al Association 2010			24			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		600		601		05			46326		European Al Association 2010			380			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		600		599		05		gt		8590		European Al Association 2010		gt		737			509				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia reticulata		F M T		451		60		11			304		Shephard 1983			201			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia reticulata		F M T		40		55		11			362		Shephard 1983			5531			1054		733			Ceriodaphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake SuperiorDI Mix
	Cladoceran Daphnia magna		S U NR		453		774		11			3900		Biesinger and Christensen 1972			338			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Daphnia magna		S M T		220		76		16			38200		Kimball 1978			2030			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Well water
	Cladoceran Daphnia magna		S U T		451		725		11			2800		Shephard 1983			287			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Daphnia magna		S U T		168		599		05			5000		European Al Association 2009		gt		227			-				Daphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Daphnia magna		S U T		168		792		05			7950		European Al Association 2009			92			333		333			Daphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Ostracod Stenocypris major		R M T		1563		651		16			310196		Shuhaimi-Othman et al 2011a			1022			1022		1022			Stenocypris		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (Malaysia)
	Amphipod Crangonyx pseudogracilis		R U T		50		675		16			9190		Martin and Holdich 1986			1217			1217		1217			Crangonyx		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (UK)
	Amphipod Hyalella azteca		F M T		105		613		048		gt		5997		Wang et al 2016 Wang et al 2017		gt		2800		gt		2800		2800		gt		Hyalella		Invert		Author reported
	Stonefly Acroneuria sp		S M T		474		746		11		gt		22600		Call et al 1984		gt		2050		gt		2050		2050		gt		Acroneuria		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Midge Chironomus plumosus		S U T		80		70		16			30000		Fargasova 2001			2536			2536		2536			Chironomus		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (Slovakia)
	Midge Paratanytarsus dissimilis		S M T		1743		728		28		gt		77700		Lamb and Bailey 1981 1983		gt		6232		gt		6232		6232		gt		Paratanytarsus		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Liberty Lake Washington
	Rainbow trout Oncorhynchus mykiss		F M T		274		758		05		gt		9840		Gundersen et al 1994		gt		1256			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		452		762		05		gt		8070		Gundersen et al 1994		gt		1140			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		895		758		05		gt		8160		Gundersen et al 1994		gt		1489			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		1304		758		05		gt		8200		Gundersen et al 1994		gt		1676			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		232		825		05			6170		Gundersen et al 1994			260			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		350		826		05			6170		Gundersen et al 1994			333			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		836		829		05			7670		Gundersen et al 1994			701			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		1158		830		05			6930		Gundersen et al 1994			777			801		801			Oncorhynchus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Atlantic salmon Salmo salar		S U T		68		55		05			584		Hamilton and Haines 1995			4602			-				Salmo			Value is from 2007 FW Cu AWQC Appendix C recommendation		Soft recon
	Atlantic salmon Salmo salar		S U T		68		65		05			599		Hamilton and Haines 1995			531			1564		1564			Salmo		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Soft recon
	Brook trout Salvelinus fontinalis		S U T		40		56		16			6530		Tandjung 1982			6947			-				Salvelinus			Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Brook trout Salvelinus fontinalis		S U T		18		56		16			3400		Tandjung 1982			6209			-				Salvelinus			Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Brook trout Salvelinus fontinalis		S U T		2		56		16			370		Tandjung 1982			2988			5051		5051			Salvelinus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Green sunfish Lepomis cyanellus		S M T		474		755		11		gt		50000		Call et al 1984		gt		5255		gt		5255		5255		gt		Lepomis		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Rio Grande silvery minnow Hybognathus amarus		R M T		140		81		05		gt		59100		Buhl 2002		gt		8620		gt		8620		8620		gt		Hybognathus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon water
	Fathead minnow Pimephales promelas		S M T		474		761		11		gt		48200		Call et al 1984		gt		4707			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Fathead minnow Pimephales promelas		S M T		474		805		11		gt		49800		Call et al 1984		gt		2837			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Fathead minnow Pimephales promelas		R M T		26		78		05			1160		ENSR 1992c			104			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		46		76		05			8180		ENSR 1992c			1191			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		96		81		05			20300		ENSR 1992c			2398			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		194		81		05			44800		ENSR 1992c			7839			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		140		81		05		gt		59100		Buhl 2002		gt		8620			2224		2224			Pimephales		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon water
	Smallmouth bass Micropterus dolomieui		S M T		1245		505		16			130		Kane and Rabeni 1987			859			859		859			Micropterus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Green tree frog Hyla cinerea		R M T		455		549		05		gt		4052		Jung and Jagoe 1995		gt		4377		gt		4377		4377		gt		Hyla		Amphibian		Value is from 2007 FW Cu AWQC Appendix C recommendation		artificial soft recon

	Normalization Chemistry
	pH		6					FAV		360
	Hardness		25					CMC		180
	DOC		1					CCC		99
	Acute Ranked GMAV											Chronic Ranked GMCV
	Rank			GMAV		Genus								Rank			GMCV		Genus
	18		gt		8620		Hybognathus		Fish							12		gt		2131		Rana		Amphib - Other Data
	17		gt		6232		Paratanytarsus		Invert							11			1710		Aeolosoma		Invert
	16		gt		5255		Lepomis		Fish							10			591		Chironomus		Invert
	15			5051		Salvelinus		Fish							9			544		Pimephales		Fish
	14		gt		4377		Hyla		Amphibian							8			488		Lymnaea		Mollusk
	13			3546		Physa		Mollusk							7			255		Brachionus		Invert
	12		gt		2983		Lampsilis		Mollusk							6			241		Danio		Fish
	11		gt		2800		Hyalella		Invert							5			171		Hyalella		Invert
	10			2536		Chironomus		Invert							4			129		Salvelinus		Fish
	9			2224		Pimephales		Fish							3			118		Ceriodaphnia		Invert
	8		gt		2050		Acroneuria		Invert							2			111		Salmo		Fish
	7			1564		Salmo		Fish							1			104		Lampsilis		Mollusk
	6			1217		Crangonyx		Invert
	5			1022		Stenocypris		Invert
	4			859		Micropterus		Fish
	3			801		Oncorhynchus		Fish
	2			733		Ceriodaphnia		Invert
	1			333		Daphnia		Invert
		N		Rank		Genus		GMAV		ln(GMAV)		ln(GMAV)2		P=R(N+1)		sqrt(P)				N		Rank		Genus		GMCV		ln(GMCV)		ln(GMCV)2		P=R(N+1)		sqrt(P)
		18		4		Micropterus		859		676		4564		0211		0459				12		4		Salvelinus		129		486		2364		0308		0555
			3		Oncorhynchus		801		669		4469		0158		0397					3		Ceriodaphnia		118		477		2277		0231		0480
			2		Ceriodaphnia		733		660		4352		0105		0324					2		Salmo		111		471		2220		0154		0392
			1		Daphnia		333		581		3374		0053		0229					1		Lampsilis		104		465		2159		0077		0277
			Sum				2585		1676		0526		141					Sum				1899		902		0769		170
			S2 =		1987										S2 =		059
			L =		4890										L =		4420
			A =		5887										A =		4592
			FAV =		360										FCV =		987
			CMC =		180

				All concentrations reported are microgL total Aluminum
				lt-----Click Button after entering data
	ENTER DATA HERE									ERRORNUM		ACUTE														CHRONIC
										Rank 1				Rank 2				Rank 3				Rank 4					Rank 1				Rank 2				Rank 3				Rank 4
	SiteName		DOC(mgL)		Hardness(mgL as CaCO3)		pH			FAV		CMC		CCC			Genus		GMAV			Genus		GMAV			Genus		GMAV			Genus		GMAV				Genus		GMCV			Genus		GMCV			Genus		GMCV			Genus		GMCV
	Site 1		50		25		9		75428737034162		35229324666008		1800		770			Daphnia			33452		Ceriodaphnia			73576		Stenocypris			102576		Crangonyx			122230			Lampsilis			10463		Ceriodaphnia			11865		Hyalella			17200		Brachionus			25648
	Site 2		50		50		9		75428737034162		31204991509063		1600		680			Daphnia			29631		Ceriodaphnia			65171		Stenocypris			90859		Crangonyx			108267			Lampsilis			9268		Ceriodaphnia			10510		Hyalella			15236		Brachionus			22718
	Site 3		50		75		9		75428737034162		29067527928199		1500		640			Daphnia			27601		Ceriodaphnia			60707		Stenocypris			84635		Crangonyx			100851			Lampsilis			8633		Ceriodaphnia			9790		Hyalella			14192		Brachionus			21162
	Site 4		50		100		9		75428737034162		27640367912592		1400		600			Daphnia			26246		Ceriodaphnia			57726		Stenocypris			80480		Crangonyx			95899			Lampsilis			8209		Ceriodaphnia			9309		Hyalella			13495		Brachionus			20123
	Site 5		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 6		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 7		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 8		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 9		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 10		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 11		50		25		85		75428737034162		63245275664384		3200		1400			Daphnia			60055		Ceriodaphnia			132086		Stenocypris			184149		Crangonyx			219432			Lampsilis			18783		Ceriodaphnia			21301		Hyalella			30879		Brachionus			46045
	Site 12		50		50		85		75428737034162		61388044609926		3100		1300			Daphnia			58291		Ceriodaphnia			128208		Stenocypris			178742		Crangonyx			212988			Lampsilis			18232		Ceriodaphnia			20676		Hyalella			29972		Brachionus			44693
	Site 13		50		75		85		75428737034162		60327020429132		3000		1300			Daphnia			57284		Ceriodaphnia			125992		Stenocypris			175652		Crangonyx			209307			Lampsilis			17917		Ceriodaphnia			20318		Hyalella			29454		Brachionus			43920
	Site 14		50		100		85		75428737034162		59585352130146		3000		1300			Daphnia			56580		Ceriodaphnia			124443		Stenocypris			173493		Crangonyx			206734			Lampsilis			17696		Ceriodaphnia			20068		Hyalella			29092		Salmo			41264
	Site 15		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 16		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 17		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 18		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 19		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 20		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370

	Appendix C Acceptable Chronic Toxicity Data of Aluminum to Freshwater Aquatic Animals
									MLR equation
	Species		Hardness (mgL as CaCO3)		pH		DOC(mgL)		EC20 Endpoint			EC20(microgL)			Normalized Conc- EC20(microgL)			SMCV-EC20(microgL)		GMCV-EC20(microgL)			Genus			DOC Notes		Dilution water
	Oligochaete Aeolosoma sp		48		6		025		Reproduction (population size)			1235			17098			17098		17098			Aeolosoma		Invert		Author reported half the detection limit
	Rotifer Brachionus calyciflorus		100		62		025		Reproduction (population size)			431			2555			2555		2555			Brachionus		Invert		Author reported half the detection limit
	Great pond snail Lymnaea stagnalis		117		61		025		Biomass			746			4877			4877		4877			Lymnaea		Mollusk		Author reported half the detection limit
	Fatmucket Lampsilis siliquoidea		1055		604		040		Biomass			169			1042			1042		1042			Lampsilis		Mollusk		Author reported
	Cladoceran Ceriodaphnia dubia		50		715		11		Reproduction - youngstarting adult			1780			1898						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		26		73		05		Reproduction - youngfemale			1557			2718						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		46		75		05		Reproduction - youngfemale			8087			1102						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		96		79		05		Reproduction - youngfemale			6519			796						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		194		81		05		Reproduction - youngfemale			6836			888						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		255		703		05		Reproduction (youngfemale)			250			563						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		122		713		05		Reproduction (youngfemale)			860			1061						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		25		798		05		Reproduction (youngfemale)			700			1006						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		61		803		05		Reproduction (youngfemale)			1010			1363						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		122		810		05		Reproduction (youngfemale)			870			1164						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		255		633		05		Reproduction (youngfemale)			260			1758						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		121		636		05		Reproduction (youngfemale)			390			1101			1182		1182			Ceriodaphnia		Invert		Author reported
	Amphipod Hyalella azteca		95		61		051		Biomass (28 d)			1993			1014						Hyalella			Author reported
	Amphipod Hyalella azteca		106		604		033		Biomass (28 d)			425			2895			1713		1713			Hyalella		Invert		Author reported
	Midge Chironomus riparius		118		558		18		Adult midge emergence			2955			1192						Chironomus			Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Midge Chironomus riparius		119		505		18		Adult midge emergence			8442			22578						Chironomus			Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Midge Chironomus riparius		91		66		051		Reproduction ( of eggscase)			3387			7664			5908		5908			Chironomus		Invert		Author reported
	Atlantic salmon Salmo salar		127		57		18		Biomass			6156			5085			5085		5085			Salmo		Fish		Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Brook trout Salvelinus fontinalis		123		655		19		Biomass			1644			270						Salvelinus			Author reported		wellRO mix
	Brook trout Salvelinus fontinalis		128		565		18		Biomass			1435			1295			5914		5914			Salvelinus		Fish		Author reported		wellRO mix
	Fathead minnow Pimephales promelas		220		770		16		Biomass			6194			3569						Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		well water
	Fathead minnow Pimephales promelas		96		620		025		Fry survival			4286			1734			2488		2488			Pimephales		Fish		Author reported half the detection limit
	Zebrafish Danio rerio		83		61		025		Biomass			2344			1102			1102		1102			Danio		Fish		Author reported half the detection limit
	Wood frog Rana sylvatica		115		469		16		MATC-survival		gt		2000		gt		9746		gt		9746		9746		gt		Rana		Amphib - Other Data		Value is from 2007 FW Cu AWQC Appendix C recommendation		aged tap water

	Appendix A Acceptable Acute Toxicity Data of Aluminum to Freshwater Aquatic Animals
	Species		Methoda		Hardness (mgL as CaCO3)		pH		DOC(mgL)			LC50 or EC50 Conc (microgL)		Reference			Normalized Conc - LC50(microgL)			Species Mean Acute Value(microgL)		Genus Mean Acute Value(microgL)			Genus			DOC Note		Dilution water
	Snail Physa sp		S M T		474		659		11		gt		23400		Call 1984 Call et al 1984		gt		48425			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		755		11			30600		Call 1984 Call et al 1984			26986			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		817		11		gt		24700		Call 1984 Call et al 1984		gt		24040			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		746		11			55500		Call 1984 Call et al 1984			50338			35462		35462			Physa		Mollusk		Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Fatmucket Lampsilis siliquoidea		F M T		1060		612		048		gt		6302		Wang et al 2016 Wang et al 2017		gt		29834		gt		29834		29834		gt		Lampsilis		Mollusk		Author reported
	Cladoceran Ceriodaphnia dubia		S M A		500		742		11			1900		McCauley et al 1986			1728			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		S M A		505		786		11			1500		McCauley et al 1986			1294			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		UW-S Lake Superior lab water
	Cladoceran Ceriodaphnia dubia		S M A		500		813		11			2560		McCauley et al 1986			2431			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		R M T		26		75		05			720		ENSR 1992d			1113			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		R M T		46		76		05			1880		ENSR 1992d			2495			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		R M T		96		78		05			2450		ENSR 1992d			2894			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		S U NR		90		715		05			3727		Fort and Stover 1995			5002			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U NR		90		715		05			5673		Fort and Stover 1995			7613			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U NR		89		76		05			2880		Soucek et al 2001			3362			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		601		05			7112		European Al Association 2009			1691			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		605		2			6865		European Al Association 2009			7088			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		609		4			15581		European Al Association 2009			10072			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		601		05			681		European Al Association 2009			1620			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		603		05			1630		European Al Association 2009			3674			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		597		05			1785		European Al Association 2009			4732			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		592		05			1410		European Al Association 2009			4295			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		699		05		gt		1300		European Al Association 2009		gt		4189			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		785		05		gt		5000		European Al Association 2009		gt		7960			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		68		2		gt		10000		European Al Association 2009		gt		20755			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		782		2		gt		15000		European Al Association 2009		gt		11626			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		677		4		gt		10000		European Al Association 2009		gt		15161			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		766		4		gt		15000		European Al Association 2009		gt		8608			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		79		05		gt		2000		European Al Association 2009		gt		3150			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		789		05		gt		2000		European Al Association 2009		gt		3156			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		604		05			1108		European Al Association 2009			850			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		598		2			11371		European Al Association 2009			4817			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		573		4			80467		European Al Association 2009			43570			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		671		05		gt		10000		European Al Association 2009		gt		23786			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		783		05		gt		5000		European Al Association 2009		gt		6344			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		679		2		gt		10000		European Al Association 2009		gt		10394			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		767		2		gt		15000		European Al Association 2009		gt		9082			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		668		4		gt		15000		European Al Association 2009		gt		12461			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		762		4		gt		15000		European Al Association 2009		gt		6332			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		606		2			33868		European Al Association 2009			7920			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		56		4			104842		European Al Association 2009			48800			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		693		05		gt		5000		European Al Association 2009		gt		7126			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		120		788		05		gt		5000		European Al Association 2009		gt		5898			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		120		676		2		gt		15000		European Al Association 2009		gt		12117			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		771		2		gt		15000		European Al Association 2009		gt		8096			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		66		4		gt		15000		European Al Association 2009		gt		10139			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		76		4		gt		15000		European Al Association 2009		gt		5545			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			11971		European Al Association 2010			2699			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			27478		European Al Association 2010			6194			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			11998		European Al Association 2010			2705			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		607		05			92495		European Al Association 2010			1876			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		609		05			31337		European Al Association 2010			6035			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		61		05			33235		European Al Association 2010			6238			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		708		05		gt		8864		European Al Association 2010		gt		2534			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		779		05		gt		42783		European Al Association 2010		gt		6930			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		753		05			13204		European Al Association 2010			244			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		600		601		05			46326		European Al Association 2010			3798			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		600		599		05		gt		8590		European Al Association 2010		gt		7366			5094				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia reticulata		F M T		451		60		11			304		Shephard 1983			2009			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia reticulata		F M T		40		55		11			362		Shephard 1983			55307			10542		7328			Ceriodaphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake SuperiorDI Mix
	Cladoceran Daphnia magna		S U NR		453		774		11			3900		Biesinger and Christensen 1972			3382			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Daphnia magna		S M T		220		76		16			38200		Kimball 1978			20303			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Well water
	Cladoceran Daphnia magna		S U T		451		725		11			2800		Shephard 1983			2868			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Daphnia magna		S U T		168		599		05			5000		European Al Association 2009		gt		2265			-				Daphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Daphnia magna		S U T		168		792		05			7950		European Al Association 2009			920			3332		3332			Daphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Ostracod Stenocypris major		R M T		1563		651		16			310196		Shuhaimi-Othman et al 2011a			10216			10216		10216			Stenocypris		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (Malaysia)
	Amphipod Crangonyx pseudogracilis		R U T		50		675		16			9190		Martin and Holdich 1986			12174			12174		12174			Crangonyx		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (UK)
	Amphipod Hyalella azteca		F M T		105		613		048		gt		5997		Wang et al 2016 Wang et al 2017		gt		28002		gt		28002		28002		gt		Hyalella		Invert		Author reported
	Stonefly Acroneuria sp		S M T		474		746		11		gt		22600		Call et al 1984		gt		20498		gt		20498		20498		gt		Acroneuria		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Midge Chironomus plumosus		S U T		80		70		16			30000		Fargasova 2001			25361			25361		25361			Chironomus		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (Slovakia)
	Midge Paratanytarsus dissimilis		S M T		1743		728		28		gt		77700		Lamb and Bailey 1981 1983		gt		62318		gt		62318		62318		gt		Paratanytarsus		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Liberty Lake Washington
	Rainbow trout Oncorhynchus mykiss		F M T		274		758		05		gt		9840		Gundersen et al 1994		gt		5745			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		452		762		05		gt		8070		Gundersen et al 1994		gt		5212			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		895		758		05		gt		8160		Gundersen et al 1994		gt		6807			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		1304		758		05		gt		8200		Gundersen et al 1994		gt		7662			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		232		825		05			6170		Gundersen et al 1994			1190			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		350		826		05			6170		Gundersen et al 1994			1523			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		836		829		05			7670		Gundersen et al 1994			3205			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		1158		830		05			6930		Gundersen et al 1994			3554			3661		3661			Oncorhynchus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Atlantic salmon Salmo salar		S U T		68		55		05			584		Hamilton and Haines 1995			21042			-				Salmo			Value is from 2007 FW Cu AWQC Appendix C recommendation		Soft recon
	Atlantic salmon Salmo salar		S U T		68		65		05			599		Hamilton and Haines 1995			2430			7151		7151			Salmo		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Soft recon
	Brook trout Salvelinus fontinalis		S U T		40		56		16			6530		Tandjung 1982			31767			-				Salvelinus			Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Brook trout Salvelinus fontinalis		S U T		18		56		16			3400		Tandjung 1982			28391			-				Salvelinus			Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Brook trout Salvelinus fontinalis		S U T		2		56		16			370		Tandjung 1982			13663			23097		23097			Salvelinus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Green sunfish Lepomis cyanellus		S M T		474		755		11		gt		50000		Call et al 1984		gt		24028		gt		24028		24028		gt		Lepomis		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Rio Grande silvery minnow Hybognathus amarus		R M T		140		81		05		gt		59100		Buhl 2002		gt		39414		gt		39414		39414		gt		Hybognathus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon water
	Fathead minnow Pimephales promelas		S M T		474		761		11		gt		48200		Call et al 1984		gt		21522			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Fathead minnow Pimephales promelas		S M T		474		805		11		gt		49800		Call et al 1984		gt		12972			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Fathead minnow Pimephales promelas		R M T		26		78		05			1160		ENSR 1992c			477			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		46		76		05			8180		ENSR 1992c			5446			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		96		81		05			20300		ENSR 1992c			10966			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		194		81		05			44800		ENSR 1992c			35847			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		140		81		05		gt		59100		Buhl 2002		gt		39414			10168		10168			Pimephales		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon water
	Smallmouth bass Micropterus dolomieui		S M T		1245		505		16			130		Kane and Rabeni 1987			3929			3929		3929			Micropterus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Green tree frog Hyla cinerea		R M T		455		549		05		gt		4052		Jung and Jagoe 1995		gt		20016		gt		20016		20016		gt		Hyla		Amphibian		Value is from 2007 FW Cu AWQC Appendix C recommendation		artificial soft recon

	Normalization Chemistry
	pH		7					FAV		2741
	Hardness		100					CMC		1400
	DOC		1					CCC		390
	Acute Ranked GMAV											Chronic Ranked GMCV
	Rank			GMAV		Genus								Rank			GMCV		Genus
	18		gt		62318		Paratanytarsus		Invert							12			17098		Aeolosoma		Invert
	17		gt		39414		Hybognathus		Fish							11		gt		9746		Rana		Amphib - Other Data
	16			35462		Physa		Mollusk							10			5908		Chironomus		Invert
	15		gt		29834		Lampsilis		Mollusk							9			4877		Lymnaea		Mollusk
	14		gt		28002		Hyalella		Invert							8			2555		Brachionus		Invert
	13			25361		Chironomus		Invert							7			2488		Pimephales		Fish
	12		gt		24028		Lepomis		Fish							6			1713		Hyalella		Invert
	11			23097		Salvelinus		Fish							5			1182		Ceriodaphnia		Invert
	10		gt		20498		Acroneuria		Invert							4			1102		Danio		Fish
	9		gt		20016		Hyla		Amphibian							3			1042		Lampsilis		Mollusk
	8			12174		Crangonyx		Invert							2			591		Salvelinus		Fish
	7			10216		Stenocypris		Invert							1			509		Salmo		Fish
	6			10168		Pimephales		Fish
	5			7328		Ceriodaphnia		Invert
	4			7151		Salmo		Fish
	3			3929		Micropterus		Fish
	2			3661		Oncorhynchus		Fish
	1			3332		Daphnia		Invert
		N		Rank		Genus		GMAV		ln(GMAV)		ln(GMAV)2		P=R(N+1)		sqrt(P)				N		Rank		Genus		GMCV		ln(GMCV)		ln(GMCV)2		P=R(N+1)		sqrt(P)
		18		4		Salmo		7151		887		7876		0211		0459				12		4		Danio		1102		700		4907		0308		0555
			3		Micropterus		3929		828		6849		0158		0397					3		Lampsilis		1042		695		4829		0231		0480
			2		Oncorhynchus		3661		821		6733		0105		0324					2		Salvelinus		591		638		4074		0154		0392
			1		Daphnia		3332		811		6579		0053		0229					1		Salmo		509		623		3883		0077		0277
			Sum				3347		2804		0526		141					Sum				2657		1769		0769		170
			S2 =		1223										S2 =		1080
			L =		7134										L =		5242
			A =		7916										A =		5976
			FAV =		2741										FCV =		3940
			CMC =		1371

				All concentrations reported are microgL total Aluminum
				lt-----Click Button after entering data
	ENTER DATA HERE									ERRORNUM		ACUTE														CHRONIC
										Rank 1				Rank 2				Rank 3				Rank 4					Rank 1				Rank 2				Rank 3				Rank 4
	SiteName		DOC(mgL)		Hardness(mgL as CaCO3)		pH			FAV		CMC		CCC			Genus		GMAV			Genus		GMAV			Genus		GMAV			Genus		GMAV				Genus		GMCV			Genus		GMCV			Genus		GMCV			Genus		GMCV
	Site 1		50		25		9		75428737034162		35229324666008		1800		770			Daphnia			33452		Ceriodaphnia			73576		Stenocypris			102576		Crangonyx			122230			Lampsilis			10463		Ceriodaphnia			11865		Hyalella			17200		Brachionus			25648
	Site 2		50		50		9		75428737034162		31204991509063		1600		680			Daphnia			29631		Ceriodaphnia			65171		Stenocypris			90859		Crangonyx			108267			Lampsilis			9268		Ceriodaphnia			10510		Hyalella			15236		Brachionus			22718
	Site 3		50		75		9		75428737034162		29067527928199		1500		640			Daphnia			27601		Ceriodaphnia			60707		Stenocypris			84635		Crangonyx			100851			Lampsilis			8633		Ceriodaphnia			9790		Hyalella			14192		Brachionus			21162
	Site 4		50		100		9		75428737034162		27640367912592		1400		600			Daphnia			26246		Ceriodaphnia			57726		Stenocypris			80480		Crangonyx			95899			Lampsilis			8209		Ceriodaphnia			9309		Hyalella			13495		Brachionus			20123
	Site 5		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 6		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 7		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 8		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 9		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 10		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 11		50		25		85		75428737034162		63245275664384		3200		1400			Daphnia			60055		Ceriodaphnia			132086		Stenocypris			184149		Crangonyx			219432			Lampsilis			18783		Ceriodaphnia			21301		Hyalella			30879		Brachionus			46045
	Site 12		50		50		85		75428737034162		61388044609926		3100		1300			Daphnia			58291		Ceriodaphnia			128208		Stenocypris			178742		Crangonyx			212988			Lampsilis			18232		Ceriodaphnia			20676		Hyalella			29972		Brachionus			44693
	Site 13		50		75		85		75428737034162		60327020429132		3000		1300			Daphnia			57284		Ceriodaphnia			125992		Stenocypris			175652		Crangonyx			209307			Lampsilis			17917		Ceriodaphnia			20318		Hyalella			29454		Brachionus			43920
	Site 14		50		100		85		75428737034162		59585352130146		3000		1300			Daphnia			56580		Ceriodaphnia			124443		Stenocypris			173493		Crangonyx			206734			Lampsilis			17696		Ceriodaphnia			20068		Hyalella			29092		Salmo			41264
	Site 15		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 16		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 17		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 18		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 19		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 20		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370

II Evaluate water quality conditions to determine applicability

bull Criteria are often based on one or more water quality conditions (eg hardness) that affect toxicity of the material to test organisms

bull Aluminum toxicity is highly dependent on the pH of the test waterndash The pH of test waters affects the amount and form

of aluminum that is bioavailable to test speciesndash Hardness and DOC (Dissolved Organic Carbon)

also influence the amount of aluminum that is bioavailable

16

Multiple Bioavailability Modeling Approaches Underwent External Peer Review

17

bull Biotic Ligand Modelsbull Full aluminum BLM (2016 model version) ndash Temperature pH

Dissolved organic carbon (DOC) Major cations (calcium [Ca] magnesium [Mg] sodium [Na] and potassium [K]) Major anions (sulfate [SO4] and chloride [Cl]) Alkalinity and Sulfide

bull Simplified aluminum BLM - pH hardness DOC temperature

bull Multilinear Regression Equationsbull pH and Hardness

Accounting for Effects of Water Chemistry on Bioavailability and Toxicity

bull Water quality criteria can be modified quantitatively when enough data are available to demonstrate that those conditions similarly affect the results with a variety of speciesndash High quality data in the draft aluminum document demonstrated

that pH DOC and hardness affected a variety of species in a similar way when exposed to aluminum concentrations

bull Recent publications by Cardwell et al (2017) and Gensemer et al (2017) summarized short-term aluminum chronic toxicity data across a range of DOC pH and hardness values for three species

18

Multiple Linear Regression (MLR) Models to Account for Effects of Water Chemistry

bull MLR models were developed for each species (DeForest et al 2017)ndash 119862119862 119889119889119906119906119887119887119894119894119886119886 11986411986411986211986220=119890119890[minus41026+[0525timesln(119863119863119874119874119862119862)]+[2201timesln(ℎ119886119886119903119903119889119889)]+(11282times119901119901119867119867)minus(0633times1199011199011198671198672)minus[0264times119901119901119867119867ln(ℎ119886119886119903119903119889119889)]]

ndash 119875119875 119901119901119903119903119900119900119898119898119890119890119897119897119886119886119904119904 11986411986411986211986220=119890119890[minus14029+[0503timesln(119863119863119874119874119862119862)]+[3443timesln(ℎ119886119886119903119903119889119889)]+(3131times119901119901119867119867)minus[0494times119901119901119867119867ln(ℎ119886119886119903119903119889119889)]]

bull These models were applied to both the acute and chronic aluminum toxicity datandash The C dubia model was applied to invertebrate datandash The P promelas model was applied to vertebrate data

19

III Normalize toxicity data in species sensitivity distribution to a set of water

quality conditionsbull Just like single parameter hardness models the MLR

models were used to normalize the aluminum toxicity data to a single set of water quality conditionsndash As an illustration for one set of calculations the draft

aluminum AWQC set these conditions to pH 7 hardness of 100 mgL and DOC of 1 mgL

bull This allows for comparisons to be made between species based on their sensitivity to aluminumndash Apples to apples

20

IV Construct sensitivity distribution to determine criteria at set conditions

21

Figure 8 Ranked Summary of Total Aluminum Genus Mean Acute Values (GMAVs) - Freshwater at pH 7 Hardness of 100 mgL and DOC of 1 mgL

IV Construct sensitivity distribution to determine criteria at set conditions

22

Figure 10 Ranked Summary of Total Aluminum Genus Mean Chronic Values (GMCVs) ndash Freshwater Supplemented with Other Data to Fulfill Missing MDRs at pH 7 Hardness of 100 mgL and DOC of 1 mgL

Aluminum Criteria Calculatorbull The relative ranking of genera in the sensitivity

distribution to which the toxicity data are normalized will change based on the set of water chemistry conditions (ie pH hardness and DOC)

bull To make calculating aluminum criteria easier EPA developed an Aluminum Criteria Calculatorndash Automated so that user only needs to enter water chemistry

conditions of interestndash Allows quick calculations for a variety of conditionsndash Limits calculations to the recommended conditions for which

the MLR models were developed were specified for hardness and DOC

bull No extrapolation beyond hardness and DOC empirical data bounds

23

Aluminum Criteria Calculator SD Example

24

pH=7 Hardness=100 DOC=1 pH=6 Hardness=25 DOC=1

Chronic Ranked GMCVRank GMCV Genus

12 17098 Aeolosoma Invert11 gt 9746 Rana Amphib - Other Data10 5908 Chironomus Invert

9 4877 Lymnaea Mollusk8 2555 Brachionus Invert7 2488 Pimephales Fish6 1713 Hyalella Invert5 1182 Ceriodaphnia Invert4 1102 Danio Fish3 1042 Lampsilis Mollusk2 591 Salvelinus Fish1 509 Salmo Fish

FCV = 3940 microgL total aluminum

Chronic Ranked GMCVRank GMCV Genus

12 gt 2131 Rana Amphib - Other Data11 1710 Aeolosoma Invert10 591 Chironomus Invert

9 544 Pimephales Fish8 488 Lymnaea Mollusk7 255 Brachionus Invert6 241 Danio Fish5 171 Hyalella Invert4 129 Salvelinus Fish3 118 Ceriodaphnia Invert2 111 Salmo Fish1 104 Lampsilis Mollusk

FCV = 987 microgL total aluminum

Multiple Scenarios

Run Scenarios

Summary Sheet

Acute Dataset

Chronic Dataset

Multiple Scenarios

Run Scenarios

Summary Sheet

Acute Dataset

Chronic Dataset

Boundaries of the Criteria Outputsbull MLR models were developed using data that

encompassndash DOC range of 008-5 mgLndash Hardness range of 98-127 mgL (as CaCO3)ndash pH range of 6-81

bull MLR criteria outputs are bounded atndash Maximum of 150 mgL total hardness (as CaCO3)

ndash DOC of 50 mgLndash pH range of the model is 50 to 90

25

Aluminum Criteria Look-Up Tables

26

bull EPA also provided criteria look-up tables in Appendix K

Har

dnes

s

CCC(microgL total aluminum)

(DOC=05 mgL)

pH 50 pH 55 pH 60 pH 65 pH 70 pH 75 pH 80 pH 85 pH 90

25 23 a 14 b 69 c 160 f 350 f 620 i 540 b 410 a 230 a

50 43 a 24 b 100 d 170 f 310 f 630 f 670 j 400 a 200 a

75 61 a 33 b 130 e 180 f 290 f 530 f 730 i 390 a 190 a

100 78 a 41 b 150 e 190 f 280 g 460 f 770 c 390 a 180 a

150 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

200 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

250 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

300 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

350 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

400 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

Aluminum Criteria Look-Up Tables

27

bull EPA also provided criteria look-up tables in Appendix K

Har

dnes

s

CCC(microgL total aluminum)

(DOC=10 mgL)

pH 50 pH 55 pH 60 pH 65 pH 70 pH 75 pH 80 pH 85 pH 90

25 33 a 21 b 99 c 220 f 490 f 900 i 780 b 590 a 330 a

50 61 a 35 b 150 e 250 f 440 f 890 f 960 j 580 a 290 a

75 88 a 47 b 180 e 260 f 410 f 740 f 1100 i 570 a 270 a

100 11 a 59 b 210 e 270 f 390 g 650 f 1100 c 560 b 260 a

150 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

200 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

250 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

300 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

350 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

400 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

Aluminum Criteria Look-Up Tables

28

bull EPA also provided criteria look-up tables in Appendix K

Har

dnes

s

CCC(microgL total aluminum)

(DOC=25 mgL)

pH 50 pH 55 pH 60 pH 65 pH 70 pH 75 pH 80 pH 85 pH 90

25 54 a 34 b 160 c 350 f 770 f 1500 i 1300 b 960 a 540 a

50 99 a 57 b 230 e 390 f 690 f 1400 f 1600 j 930 a 470 a

75 14 a 77 b 290 e 410 f 640 f 1200 f 1700 i 920 a 440 a

100 18 a 96 b 330 e 420 f 620 g 1000 f 1800 d 920 b 420 a

150 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

200 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

250 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

300 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

350 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

400 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

Aluminum Criteria Look-Up Tables

29

bull EPA also provided criteria look-up tables in Appendix K

Har

dnes

s

CCC(microgL total aluminum)

(DOC=50 mgL)

pH 50 pH 55 pH 60 pH 65 pH 70 pH 75 pH 80 pH 85 pH 90

25 77 a 49 b 230 c 500 f 1100 f 2100 c 1900 b 1400 a 770 a

50 14 a 82 b 330 e 540 f 970 f 2000 f 2200 j 1300 a 680 a

75 20 a 110 b 410 e 570 f 900 f 1600 f 2500 i 1300 a 640 a

100 26 a 140 b 460 f 590 f 880 g 1400 f 2500 d 1300 b 600 a

150 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

200 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

250 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

300 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

350 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

400 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

Status

30

bull Draft Document Released to the Federal Register on 72817ndash Public comment period of 60 days ndash Due Date for Comments is 92617

bull Revisions will be made considering public commentsbull Subsequent EPA intra-agency review of final criteria

document will then be conducted bull Publication of final criteria document planned for Late Fall

2017

Federal Register Webpagehttpswwwfederalregistergovdocuments201

707282017-15968request-for-scientific-views-draft-updated-aquatic-life-ambient-water-quality-criteria-for-aluminum

Aluminum Criteria Webpagehttpswwwepagovwqc2017-draft-aquatic-life-

criteria-aluminum-freshwater-documents

31

	Appendix C Acceptable Chronic Toxicity Data of Aluminum to Freshwater Aquatic Animals
									MLR equation
	Species		Hardness (mgL as CaCO3)		pH		DOC(mgL)		EC20 Endpoint			EC20(microgL)			Normalized Conc- EC20(microgL)			SMCV-EC20(microgL)		GMCV-EC20(microgL)			Genus			DOC Notes		Dilution water
	Oligochaete Aeolosoma sp		48		6		025		Reproduction (population size)			1235			1710			1710		1710			Aeolosoma		Invert		Author reported half the detection limit
	Rotifer Brachionus calyciflorus		100		62		025		Reproduction (population size)			431			255			255		255			Brachionus		Invert		Author reported half the detection limit
	Great pond snail Lymnaea stagnalis		117		61		025		Biomass			746			488			488		488			Lymnaea		Mollusk		Author reported half the detection limit
	Fatmucket Lampsilis siliquoidea		1055		604		040		Biomass			169			104			104		104			Lampsilis		Mollusk		Author reported
	Cladoceran Ceriodaphnia dubia		50		715		11		Reproduction - youngstarting adult			1780			190						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		26		73		05		Reproduction - youngfemale			1557			272						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		46		75		05		Reproduction - youngfemale			8087			110						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		96		79		05		Reproduction - youngfemale			6519			80						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		194		81		05		Reproduction - youngfemale			6836			89						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		255		703		05		Reproduction (youngfemale)			250			56						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		122		713		05		Reproduction (youngfemale)			860			106						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		25		798		05		Reproduction (youngfemale)			700			101						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		61		803		05		Reproduction (youngfemale)			1010			136						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		122		810		05		Reproduction (youngfemale)			870			116						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		255		633		05		Reproduction (youngfemale)			260			176						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		121		636		05		Reproduction (youngfemale)			390			110			118		118			Ceriodaphnia		Invert		Author reported
	Amphipod Hyalella azteca		95		61		051		Biomass (28 d)			1993			101						Hyalella			Author reported
	Amphipod Hyalella azteca		106		604		033		Biomass (28 d)			425			289			171		171			Hyalella		Invert		Author reported
	Midge Chironomus riparius		118		558		18		Adult midge emergence			2955			119						Chironomus			Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Midge Chironomus riparius		119		505		18		Adult midge emergence			8442			2258						Chironomus			Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Midge Chironomus riparius		91		66		051		Reproduction ( of eggscase)			3387			766			591		591			Chironomus		Invert		Author reported
	Atlantic salmon Salmo salar		127		57		18		Biomass			6156			1112			1112		1112			Salmo		Fish		Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Brook trout Salvelinus fontinalis		123		655		19		Biomass			1644			59						Salvelinus			Author reported		wellRO mix
	Brook trout Salvelinus fontinalis		128		565		18		Biomass			1435			283			1293		1293			Salvelinus		Fish		Author reported		wellRO mix
	Fathead minnow Pimephales promelas		220		770		16		Biomass			6194			781						Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		well water
	Fathead minnow Pimephales promelas		96		620		025		Fry survival			4286			379			544		544			Pimephales		Fish		Author reported half the detection limit
	Zebrafish Danio rerio		83		61		025		Biomass			2344			241			241		241			Danio		Fish		Author reported half the detection limit
	Wood frog Rana sylvatica		115		469		16		MATC-survival		gt		2000		gt		2131		gt		2131		2131		gt		Rana		Amphib - Other Data		Value is from 2007 FW Cu AWQC Appendix C recommendation		aged tap water

	Appendix A Acceptable Acute Toxicity Data of Aluminum to Freshwater Aquatic Animals
	Species		Methoda		Hardness (mgL as CaCO3)		pH		DOC(mgL)			LC50 or EC50 Conc (microgL)		Reference			Normalized Conc - LC50(microgL)			Species Mean Acute Value(microgL)		Genus Mean Acute Value(microgL)			Genus			DOC Note		Dilution water
	Snail Physa sp		S M T		474		659		11		gt		23400		Call 1984 Call et al 1984		gt		4843			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		755		11			30600		Call 1984 Call et al 1984			2699			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		817		11		gt		24700		Call 1984 Call et al 1984		gt		2404			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		746		11			55500		Call 1984 Call et al 1984			5034			3546		3546			Physa		Mollusk		Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Fatmucket Lampsilis siliquoidea		F M T		1060		612		048		gt		6302		Wang et al 2016 Wang et al 2017		gt		2983		gt		2983		2983		gt		Lampsilis		Mollusk		Author reported
	Cladoceran Ceriodaphnia dubia		S M A		500		742		11			1900		McCauley et al 1986			173			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		S M A		505		786		11			1500		McCauley et al 1986			129			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		UW-S Lake Superior lab water
	Cladoceran Ceriodaphnia dubia		S M A		500		813		11			2560		McCauley et al 1986			243			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		R M T		26		75		05			720		ENSR 1992d			111			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		R M T		46		76		05			1880		ENSR 1992d			250			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		R M T		96		78		05			2450		ENSR 1992d			289			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		S U NR		90		715		05			3727		Fort and Stover 1995			500			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U NR		90		715		05			5673		Fort and Stover 1995			761			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U NR		89		76		05			2880		Soucek et al 2001			336			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		601		05			7112		European Al Association 2009			169			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		605		2			6865		European Al Association 2009			709			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		609		4			15581		European Al Association 2009			1007			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		601		05			681		European Al Association 2009			162			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		603		05			1630		European Al Association 2009			367			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		597		05			1785		European Al Association 2009			473			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		592		05			1410		European Al Association 2009			429			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		699		05		gt		1300		European Al Association 2009		gt		419			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		785		05		gt		5000		European Al Association 2009		gt		796			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		68		2		gt		10000		European Al Association 2009		gt		2076			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		782		2		gt		15000		European Al Association 2009		gt		1163			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		677		4		gt		10000		European Al Association 2009		gt		1516			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		766		4		gt		15000		European Al Association 2009		gt		861			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		79		05		gt		2000		European Al Association 2009		gt		315			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		789		05		gt		2000		European Al Association 2009		gt		316			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		604		05			1108		European Al Association 2009			85			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		598		2			11371		European Al Association 2009			482			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		573		4			80467		European Al Association 2009			4357			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		671		05		gt		10000		European Al Association 2009		gt		2379			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		783		05		gt		5000		European Al Association 2009		gt		634			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		679		2		gt		10000		European Al Association 2009		gt		1039			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		767		2		gt		15000		European Al Association 2009		gt		908			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		668		4		gt		15000		European Al Association 2009		gt		1246			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		762		4		gt		15000		European Al Association 2009		gt		633			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		606		2			33868		European Al Association 2009			792			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		56		4			104842		European Al Association 2009			4880			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		693		05		gt		5000		European Al Association 2009		gt		713			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		120		788		05		gt		5000		European Al Association 2009		gt		590			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		120		676		2		gt		15000		European Al Association 2009		gt		1212			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		771		2		gt		15000		European Al Association 2009		gt		810			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		66		4		gt		15000		European Al Association 2009		gt		1014			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		76		4		gt		15000		European Al Association 2009		gt		555			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			11971		European Al Association 2010			270			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			27478		European Al Association 2010			619			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			11998		European Al Association 2010			270			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		607		05			92495		European Al Association 2010			188			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		609		05			31337		European Al Association 2010			604			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		61		05			33235		European Al Association 2010			624			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		708		05		gt		8864		European Al Association 2010		gt		253			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		779		05		gt		42783		European Al Association 2010		gt		693			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		753		05			13204		European Al Association 2010			24			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		600		601		05			46326		European Al Association 2010			380			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		600		599		05		gt		8590		European Al Association 2010		gt		737			509				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia reticulata		F M T		451		60		11			304		Shephard 1983			201			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia reticulata		F M T		40		55		11			362		Shephard 1983			5531			1054		733			Ceriodaphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake SuperiorDI Mix
	Cladoceran Daphnia magna		S U NR		453		774		11			3900		Biesinger and Christensen 1972			338			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Daphnia magna		S M T		220		76		16			38200		Kimball 1978			2030			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Well water
	Cladoceran Daphnia magna		S U T		451		725		11			2800		Shephard 1983			287			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Daphnia magna		S U T		168		599		05			5000		European Al Association 2009		gt		227			-				Daphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Daphnia magna		S U T		168		792		05			7950		European Al Association 2009			92			333		333			Daphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Ostracod Stenocypris major		R M T		1563		651		16			310196		Shuhaimi-Othman et al 2011a			1022			1022		1022			Stenocypris		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (Malaysia)
	Amphipod Crangonyx pseudogracilis		R U T		50		675		16			9190		Martin and Holdich 1986			1217			1217		1217			Crangonyx		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (UK)
	Amphipod Hyalella azteca		F M T		105		613		048		gt		5997		Wang et al 2016 Wang et al 2017		gt		2800		gt		2800		2800		gt		Hyalella		Invert		Author reported
	Stonefly Acroneuria sp		S M T		474		746		11		gt		22600		Call et al 1984		gt		2050		gt		2050		2050		gt		Acroneuria		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Midge Chironomus plumosus		S U T		80		70		16			30000		Fargasova 2001			2536			2536		2536			Chironomus		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (Slovakia)
	Midge Paratanytarsus dissimilis		S M T		1743		728		28		gt		77700		Lamb and Bailey 1981 1983		gt		6232		gt		6232		6232		gt		Paratanytarsus		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Liberty Lake Washington
	Rainbow trout Oncorhynchus mykiss		F M T		274		758		05		gt		9840		Gundersen et al 1994		gt		1256			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		452		762		05		gt		8070		Gundersen et al 1994		gt		1140			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		895		758		05		gt		8160		Gundersen et al 1994		gt		1489			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		1304		758		05		gt		8200		Gundersen et al 1994		gt		1676			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		232		825		05			6170		Gundersen et al 1994			260			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		350		826		05			6170		Gundersen et al 1994			333			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		836		829		05			7670		Gundersen et al 1994			701			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		1158		830		05			6930		Gundersen et al 1994			777			801		801			Oncorhynchus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Atlantic salmon Salmo salar		S U T		68		55		05			584		Hamilton and Haines 1995			4602			-				Salmo			Value is from 2007 FW Cu AWQC Appendix C recommendation		Soft recon
	Atlantic salmon Salmo salar		S U T		68		65		05			599		Hamilton and Haines 1995			531			1564		1564			Salmo		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Soft recon
	Brook trout Salvelinus fontinalis		S U T		40		56		16			6530		Tandjung 1982			6947			-				Salvelinus			Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Brook trout Salvelinus fontinalis		S U T		18		56		16			3400		Tandjung 1982			6209			-				Salvelinus			Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Brook trout Salvelinus fontinalis		S U T		2		56		16			370		Tandjung 1982			2988			5051		5051			Salvelinus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Green sunfish Lepomis cyanellus		S M T		474		755		11		gt		50000		Call et al 1984		gt		5255		gt		5255		5255		gt		Lepomis		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Rio Grande silvery minnow Hybognathus amarus		R M T		140		81		05		gt		59100		Buhl 2002		gt		8620		gt		8620		8620		gt		Hybognathus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon water
	Fathead minnow Pimephales promelas		S M T		474		761		11		gt		48200		Call et al 1984		gt		4707			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Fathead minnow Pimephales promelas		S M T		474		805		11		gt		49800		Call et al 1984		gt		2837			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Fathead minnow Pimephales promelas		R M T		26		78		05			1160		ENSR 1992c			104			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		46		76		05			8180		ENSR 1992c			1191			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		96		81		05			20300		ENSR 1992c			2398			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		194		81		05			44800		ENSR 1992c			7839			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		140		81		05		gt		59100		Buhl 2002		gt		8620			2224		2224			Pimephales		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon water
	Smallmouth bass Micropterus dolomieui		S M T		1245		505		16			130		Kane and Rabeni 1987			859			859		859			Micropterus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Green tree frog Hyla cinerea		R M T		455		549		05		gt		4052		Jung and Jagoe 1995		gt		4377		gt		4377		4377		gt		Hyla		Amphibian		Value is from 2007 FW Cu AWQC Appendix C recommendation		artificial soft recon

	Normalization Chemistry
	pH		6					FAV		360
	Hardness		25					CMC		180
	DOC		1					CCC		99
	Acute Ranked GMAV											Chronic Ranked GMCV
	Rank			GMAV		Genus								Rank			GMCV		Genus
	18		gt		8620		Hybognathus		Fish							12		gt		2131		Rana		Amphib - Other Data
	17		gt		6232		Paratanytarsus		Invert							11			1710		Aeolosoma		Invert
	16		gt		5255		Lepomis		Fish							10			591		Chironomus		Invert
	15			5051		Salvelinus		Fish							9			544		Pimephales		Fish
	14		gt		4377		Hyla		Amphibian							8			488		Lymnaea		Mollusk
	13			3546		Physa		Mollusk							7			255		Brachionus		Invert
	12		gt		2983		Lampsilis		Mollusk							6			241		Danio		Fish
	11		gt		2800		Hyalella		Invert							5			171		Hyalella		Invert
	10			2536		Chironomus		Invert							4			129		Salvelinus		Fish
	9			2224		Pimephales		Fish							3			118		Ceriodaphnia		Invert
	8		gt		2050		Acroneuria		Invert							2			111		Salmo		Fish
	7			1564		Salmo		Fish							1			104		Lampsilis		Mollusk
	6			1217		Crangonyx		Invert
	5			1022		Stenocypris		Invert
	4			859		Micropterus		Fish
	3			801		Oncorhynchus		Fish
	2			733		Ceriodaphnia		Invert
	1			333		Daphnia		Invert
		N		Rank		Genus		GMAV		ln(GMAV)		ln(GMAV)2		P=R(N+1)		sqrt(P)				N		Rank		Genus		GMCV		ln(GMCV)		ln(GMCV)2		P=R(N+1)		sqrt(P)
		18		4		Micropterus		859		676		4564		0211		0459				12		4		Salvelinus		129		486		2364		0308		0555
			3		Oncorhynchus		801		669		4469		0158		0397					3		Ceriodaphnia		118		477		2277		0231		0480
			2		Ceriodaphnia		733		660		4352		0105		0324					2		Salmo		111		471		2220		0154		0392
			1		Daphnia		333		581		3374		0053		0229					1		Lampsilis		104		465		2159		0077		0277
			Sum				2585		1676		0526		141					Sum				1899		902		0769		170
			S2 =		1987										S2 =		059
			L =		4890										L =		4420
			A =		5887										A =		4592
			FAV =		360										FCV =		987
			CMC =		180

				All concentrations reported are microgL total Aluminum
				lt-----Click Button after entering data
	ENTER DATA HERE									ERRORNUM		ACUTE														CHRONIC
										Rank 1				Rank 2				Rank 3				Rank 4					Rank 1				Rank 2				Rank 3				Rank 4
	SiteName		DOC(mgL)		Hardness(mgL as CaCO3)		pH			FAV		CMC		CCC			Genus		GMAV			Genus		GMAV			Genus		GMAV			Genus		GMAV				Genus		GMCV			Genus		GMCV			Genus		GMCV			Genus		GMCV
	Site 1		50		25		9		75428737034162		35229324666008		1800		770			Daphnia			33452		Ceriodaphnia			73576		Stenocypris			102576		Crangonyx			122230			Lampsilis			10463		Ceriodaphnia			11865		Hyalella			17200		Brachionus			25648
	Site 2		50		50		9		75428737034162		31204991509063		1600		680			Daphnia			29631		Ceriodaphnia			65171		Stenocypris			90859		Crangonyx			108267			Lampsilis			9268		Ceriodaphnia			10510		Hyalella			15236		Brachionus			22718
	Site 3		50		75		9		75428737034162		29067527928199		1500		640			Daphnia			27601		Ceriodaphnia			60707		Stenocypris			84635		Crangonyx			100851			Lampsilis			8633		Ceriodaphnia			9790		Hyalella			14192		Brachionus			21162
	Site 4		50		100		9		75428737034162		27640367912592		1400		600			Daphnia			26246		Ceriodaphnia			57726		Stenocypris			80480		Crangonyx			95899			Lampsilis			8209		Ceriodaphnia			9309		Hyalella			13495		Brachionus			20123
	Site 5		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 6		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 7		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 8		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 9		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 10		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 11		50		25		85		75428737034162		63245275664384		3200		1400			Daphnia			60055		Ceriodaphnia			132086		Stenocypris			184149		Crangonyx			219432			Lampsilis			18783		Ceriodaphnia			21301		Hyalella			30879		Brachionus			46045
	Site 12		50		50		85		75428737034162		61388044609926		3100		1300			Daphnia			58291		Ceriodaphnia			128208		Stenocypris			178742		Crangonyx			212988			Lampsilis			18232		Ceriodaphnia			20676		Hyalella			29972		Brachionus			44693
	Site 13		50		75		85		75428737034162		60327020429132		3000		1300			Daphnia			57284		Ceriodaphnia			125992		Stenocypris			175652		Crangonyx			209307			Lampsilis			17917		Ceriodaphnia			20318		Hyalella			29454		Brachionus			43920
	Site 14		50		100		85		75428737034162		59585352130146		3000		1300			Daphnia			56580		Ceriodaphnia			124443		Stenocypris			173493		Crangonyx			206734			Lampsilis			17696		Ceriodaphnia			20068		Hyalella			29092		Salmo			41264
	Site 15		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 16		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 17		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 18		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 19		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 20		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370

	Appendix C Acceptable Chronic Toxicity Data of Aluminum to Freshwater Aquatic Animals
									MLR equation
	Species		Hardness (mgL as CaCO3)		pH		DOC(mgL)		EC20 Endpoint			EC20(microgL)			Normalized Conc- EC20(microgL)			SMCV-EC20(microgL)		GMCV-EC20(microgL)			Genus			DOC Notes		Dilution water
	Oligochaete Aeolosoma sp		48		6		025		Reproduction (population size)			1235			17098			17098		17098			Aeolosoma		Invert		Author reported half the detection limit
	Rotifer Brachionus calyciflorus		100		62		025		Reproduction (population size)			431			2555			2555		2555			Brachionus		Invert		Author reported half the detection limit
	Great pond snail Lymnaea stagnalis		117		61		025		Biomass			746			4877			4877		4877			Lymnaea		Mollusk		Author reported half the detection limit
	Fatmucket Lampsilis siliquoidea		1055		604		040		Biomass			169			1042			1042		1042			Lampsilis		Mollusk		Author reported
	Cladoceran Ceriodaphnia dubia		50		715		11		Reproduction - youngstarting adult			1780			1898						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		26		73		05		Reproduction - youngfemale			1557			2718						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		46		75		05		Reproduction - youngfemale			8087			1102						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		96		79		05		Reproduction - youngfemale			6519			796						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		194		81		05		Reproduction - youngfemale			6836			888						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		255		703		05		Reproduction (youngfemale)			250			563						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		122		713		05		Reproduction (youngfemale)			860			1061						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		25		798		05		Reproduction (youngfemale)			700			1006						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		61		803		05		Reproduction (youngfemale)			1010			1363						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		122		810		05		Reproduction (youngfemale)			870			1164						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		255		633		05		Reproduction (youngfemale)			260			1758						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		121		636		05		Reproduction (youngfemale)			390			1101			1182		1182			Ceriodaphnia		Invert		Author reported
	Amphipod Hyalella azteca		95		61		051		Biomass (28 d)			1993			1014						Hyalella			Author reported
	Amphipod Hyalella azteca		106		604		033		Biomass (28 d)			425			2895			1713		1713			Hyalella		Invert		Author reported
	Midge Chironomus riparius		118		558		18		Adult midge emergence			2955			1192						Chironomus			Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Midge Chironomus riparius		119		505		18		Adult midge emergence			8442			22578						Chironomus			Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Midge Chironomus riparius		91		66		051		Reproduction ( of eggscase)			3387			7664			5908		5908			Chironomus		Invert		Author reported
	Atlantic salmon Salmo salar		127		57		18		Biomass			6156			5085			5085		5085			Salmo		Fish		Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Brook trout Salvelinus fontinalis		123		655		19		Biomass			1644			270						Salvelinus			Author reported		wellRO mix
	Brook trout Salvelinus fontinalis		128		565		18		Biomass			1435			1295			5914		5914			Salvelinus		Fish		Author reported		wellRO mix
	Fathead minnow Pimephales promelas		220		770		16		Biomass			6194			3569						Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		well water
	Fathead minnow Pimephales promelas		96		620		025		Fry survival			4286			1734			2488		2488			Pimephales		Fish		Author reported half the detection limit
	Zebrafish Danio rerio		83		61		025		Biomass			2344			1102			1102		1102			Danio		Fish		Author reported half the detection limit
	Wood frog Rana sylvatica		115		469		16		MATC-survival		gt		2000		gt		9746		gt		9746		9746		gt		Rana		Amphib - Other Data		Value is from 2007 FW Cu AWQC Appendix C recommendation		aged tap water

	Appendix A Acceptable Acute Toxicity Data of Aluminum to Freshwater Aquatic Animals
	Species		Methoda		Hardness (mgL as CaCO3)		pH		DOC(mgL)			LC50 or EC50 Conc (microgL)		Reference			Normalized Conc - LC50(microgL)			Species Mean Acute Value(microgL)		Genus Mean Acute Value(microgL)			Genus			DOC Note		Dilution water
	Snail Physa sp		S M T		474		659		11		gt		23400		Call 1984 Call et al 1984		gt		48425			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		755		11			30600		Call 1984 Call et al 1984			26986			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		817		11		gt		24700		Call 1984 Call et al 1984		gt		24040			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		746		11			55500		Call 1984 Call et al 1984			50338			35462		35462			Physa		Mollusk		Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Fatmucket Lampsilis siliquoidea		F M T		1060		612		048		gt		6302		Wang et al 2016 Wang et al 2017		gt		29834		gt		29834		29834		gt		Lampsilis		Mollusk		Author reported
	Cladoceran Ceriodaphnia dubia		S M A		500		742		11			1900		McCauley et al 1986			1728			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		S M A		505		786		11			1500		McCauley et al 1986			1294			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		UW-S Lake Superior lab water
	Cladoceran Ceriodaphnia dubia		S M A		500		813		11			2560		McCauley et al 1986			2431			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		R M T		26		75		05			720		ENSR 1992d			1113			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		R M T		46		76		05			1880		ENSR 1992d			2495			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		R M T		96		78		05			2450		ENSR 1992d			2894			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		S U NR		90		715		05			3727		Fort and Stover 1995			5002			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U NR		90		715		05			5673		Fort and Stover 1995			7613			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U NR		89		76		05			2880		Soucek et al 2001			3362			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		601		05			7112		European Al Association 2009			1691			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		605		2			6865		European Al Association 2009			7088			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		609		4			15581		European Al Association 2009			10072			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		601		05			681		European Al Association 2009			1620			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		603		05			1630		European Al Association 2009			3674			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		597		05			1785		European Al Association 2009			4732			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		592		05			1410		European Al Association 2009			4295			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		699		05		gt		1300		European Al Association 2009		gt		4189			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		785		05		gt		5000		European Al Association 2009		gt		7960			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		68		2		gt		10000		European Al Association 2009		gt		20755			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		782		2		gt		15000		European Al Association 2009		gt		11626			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		677		4		gt		10000		European Al Association 2009		gt		15161			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		766		4		gt		15000		European Al Association 2009		gt		8608			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		79		05		gt		2000		European Al Association 2009		gt		3150			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		789		05		gt		2000		European Al Association 2009		gt		3156			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		604		05			1108		European Al Association 2009			850			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		598		2			11371		European Al Association 2009			4817			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		573		4			80467		European Al Association 2009			43570			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		671		05		gt		10000		European Al Association 2009		gt		23786			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		783		05		gt		5000		European Al Association 2009		gt		6344			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		679		2		gt		10000		European Al Association 2009		gt		10394			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		767		2		gt		15000		European Al Association 2009		gt		9082			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		668		4		gt		15000		European Al Association 2009		gt		12461			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		762		4		gt		15000		European Al Association 2009		gt		6332			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		606		2			33868		European Al Association 2009			7920			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		56		4			104842		European Al Association 2009			48800			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		693		05		gt		5000		European Al Association 2009		gt		7126			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		120		788		05		gt		5000		European Al Association 2009		gt		5898			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		120		676		2		gt		15000		European Al Association 2009		gt		12117			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		771		2		gt		15000		European Al Association 2009		gt		8096			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		66		4		gt		15000		European Al Association 2009		gt		10139			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		76		4		gt		15000		European Al Association 2009		gt		5545			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			11971		European Al Association 2010			2699			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			27478		European Al Association 2010			6194			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			11998		European Al Association 2010			2705			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		607		05			92495		European Al Association 2010			1876			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		609		05			31337		European Al Association 2010			6035			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		61		05			33235		European Al Association 2010			6238			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		708		05		gt		8864		European Al Association 2010		gt		2534			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		779		05		gt		42783		European Al Association 2010		gt		6930			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		753		05			13204		European Al Association 2010			244			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		600		601		05			46326		European Al Association 2010			3798			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		600		599		05		gt		8590		European Al Association 2010		gt		7366			5094				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia reticulata		F M T		451		60		11			304		Shephard 1983			2009			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia reticulata		F M T		40		55		11			362		Shephard 1983			55307			10542		7328			Ceriodaphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake SuperiorDI Mix
	Cladoceran Daphnia magna		S U NR		453		774		11			3900		Biesinger and Christensen 1972			3382			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Daphnia magna		S M T		220		76		16			38200		Kimball 1978			20303			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Well water
	Cladoceran Daphnia magna		S U T		451		725		11			2800		Shephard 1983			2868			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Daphnia magna		S U T		168		599		05			5000		European Al Association 2009		gt		2265			-				Daphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Daphnia magna		S U T		168		792		05			7950		European Al Association 2009			920			3332		3332			Daphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Ostracod Stenocypris major		R M T		1563		651		16			310196		Shuhaimi-Othman et al 2011a			10216			10216		10216			Stenocypris		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (Malaysia)
	Amphipod Crangonyx pseudogracilis		R U T		50		675		16			9190		Martin and Holdich 1986			12174			12174		12174			Crangonyx		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (UK)
	Amphipod Hyalella azteca		F M T		105		613		048		gt		5997		Wang et al 2016 Wang et al 2017		gt		28002		gt		28002		28002		gt		Hyalella		Invert		Author reported
	Stonefly Acroneuria sp		S M T		474		746		11		gt		22600		Call et al 1984		gt		20498		gt		20498		20498		gt		Acroneuria		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Midge Chironomus plumosus		S U T		80		70		16			30000		Fargasova 2001			25361			25361		25361			Chironomus		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (Slovakia)
	Midge Paratanytarsus dissimilis		S M T		1743		728		28		gt		77700		Lamb and Bailey 1981 1983		gt		62318		gt		62318		62318		gt		Paratanytarsus		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Liberty Lake Washington
	Rainbow trout Oncorhynchus mykiss		F M T		274		758		05		gt		9840		Gundersen et al 1994		gt		5745			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		452		762		05		gt		8070		Gundersen et al 1994		gt		5212			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		895		758		05		gt		8160		Gundersen et al 1994		gt		6807			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		1304		758		05		gt		8200		Gundersen et al 1994		gt		7662			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		232		825		05			6170		Gundersen et al 1994			1190			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		350		826		05			6170		Gundersen et al 1994			1523			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		836		829		05			7670		Gundersen et al 1994			3205			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		1158		830		05			6930		Gundersen et al 1994			3554			3661		3661			Oncorhynchus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Atlantic salmon Salmo salar		S U T		68		55		05			584		Hamilton and Haines 1995			21042			-				Salmo			Value is from 2007 FW Cu AWQC Appendix C recommendation		Soft recon
	Atlantic salmon Salmo salar		S U T		68		65		05			599		Hamilton and Haines 1995			2430			7151		7151			Salmo		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Soft recon
	Brook trout Salvelinus fontinalis		S U T		40		56		16			6530		Tandjung 1982			31767			-				Salvelinus			Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Brook trout Salvelinus fontinalis		S U T		18		56		16			3400		Tandjung 1982			28391			-				Salvelinus			Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Brook trout Salvelinus fontinalis		S U T		2		56		16			370		Tandjung 1982			13663			23097		23097			Salvelinus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Green sunfish Lepomis cyanellus		S M T		474		755		11		gt		50000		Call et al 1984		gt		24028		gt		24028		24028		gt		Lepomis		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Rio Grande silvery minnow Hybognathus amarus		R M T		140		81		05		gt		59100		Buhl 2002		gt		39414		gt		39414		39414		gt		Hybognathus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon water
	Fathead minnow Pimephales promelas		S M T		474		761		11		gt		48200		Call et al 1984		gt		21522			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Fathead minnow Pimephales promelas		S M T		474		805		11		gt		49800		Call et al 1984		gt		12972			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Fathead minnow Pimephales promelas		R M T		26		78		05			1160		ENSR 1992c			477			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		46		76		05			8180		ENSR 1992c			5446			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		96		81		05			20300		ENSR 1992c			10966			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		194		81		05			44800		ENSR 1992c			35847			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		140		81		05		gt		59100		Buhl 2002		gt		39414			10168		10168			Pimephales		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon water
	Smallmouth bass Micropterus dolomieui		S M T		1245		505		16			130		Kane and Rabeni 1987			3929			3929		3929			Micropterus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Green tree frog Hyla cinerea		R M T		455		549		05		gt		4052		Jung and Jagoe 1995		gt		20016		gt		20016		20016		gt		Hyla		Amphibian		Value is from 2007 FW Cu AWQC Appendix C recommendation		artificial soft recon

	Normalization Chemistry
	pH		7					FAV		2741
	Hardness		100					CMC		1400
	DOC		1					CCC		390
	Acute Ranked GMAV											Chronic Ranked GMCV
	Rank			GMAV		Genus								Rank			GMCV		Genus
	18		gt		62318		Paratanytarsus		Invert							12			17098		Aeolosoma		Invert
	17		gt		39414		Hybognathus		Fish							11		gt		9746		Rana		Amphib - Other Data
	16			35462		Physa		Mollusk							10			5908		Chironomus		Invert
	15		gt		29834		Lampsilis		Mollusk							9			4877		Lymnaea		Mollusk
	14		gt		28002		Hyalella		Invert							8			2555		Brachionus		Invert
	13			25361		Chironomus		Invert							7			2488		Pimephales		Fish
	12		gt		24028		Lepomis		Fish							6			1713		Hyalella		Invert
	11			23097		Salvelinus		Fish							5			1182		Ceriodaphnia		Invert
	10		gt		20498		Acroneuria		Invert							4			1102		Danio		Fish
	9		gt		20016		Hyla		Amphibian							3			1042		Lampsilis		Mollusk
	8			12174		Crangonyx		Invert							2			591		Salvelinus		Fish
	7			10216		Stenocypris		Invert							1			509		Salmo		Fish
	6			10168		Pimephales		Fish
	5			7328		Ceriodaphnia		Invert
	4			7151		Salmo		Fish
	3			3929		Micropterus		Fish
	2			3661		Oncorhynchus		Fish
	1			3332		Daphnia		Invert
		N		Rank		Genus		GMAV		ln(GMAV)		ln(GMAV)2		P=R(N+1)		sqrt(P)				N		Rank		Genus		GMCV		ln(GMCV)		ln(GMCV)2		P=R(N+1)		sqrt(P)
		18		4		Salmo		7151		887		7876		0211		0459				12		4		Danio		1102		700		4907		0308		0555
			3		Micropterus		3929		828		6849		0158		0397					3		Lampsilis		1042		695		4829		0231		0480
			2		Oncorhynchus		3661		821		6733		0105		0324					2		Salvelinus		591		638		4074		0154		0392
			1		Daphnia		3332		811		6579		0053		0229					1		Salmo		509		623		3883		0077		0277
			Sum				3347		2804		0526		141					Sum				2657		1769		0769		170
			S2 =		1223										S2 =		1080
			L =		7134										L =		5242
			A =		7916										A =		5976
			FAV =		2741										FCV =		3940
			CMC =		1371

				All concentrations reported are microgL total Aluminum
				lt-----Click Button after entering data
	ENTER DATA HERE									ERRORNUM		ACUTE														CHRONIC
										Rank 1				Rank 2				Rank 3				Rank 4					Rank 1				Rank 2				Rank 3				Rank 4
	SiteName		DOC(mgL)		Hardness(mgL as CaCO3)		pH			FAV		CMC		CCC			Genus		GMAV			Genus		GMAV			Genus		GMAV			Genus		GMAV				Genus		GMCV			Genus		GMCV			Genus		GMCV			Genus		GMCV
	Site 1		50		25		9		75428737034162		35229324666008		1800		770			Daphnia			33452		Ceriodaphnia			73576		Stenocypris			102576		Crangonyx			122230			Lampsilis			10463		Ceriodaphnia			11865		Hyalella			17200		Brachionus			25648
	Site 2		50		50		9		75428737034162		31204991509063		1600		680			Daphnia			29631		Ceriodaphnia			65171		Stenocypris			90859		Crangonyx			108267			Lampsilis			9268		Ceriodaphnia			10510		Hyalella			15236		Brachionus			22718
	Site 3		50		75		9		75428737034162		29067527928199		1500		640			Daphnia			27601		Ceriodaphnia			60707		Stenocypris			84635		Crangonyx			100851			Lampsilis			8633		Ceriodaphnia			9790		Hyalella			14192		Brachionus			21162
	Site 4		50		100		9		75428737034162		27640367912592		1400		600			Daphnia			26246		Ceriodaphnia			57726		Stenocypris			80480		Crangonyx			95899			Lampsilis			8209		Ceriodaphnia			9309		Hyalella			13495		Brachionus			20123
	Site 5		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 6		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 7		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 8		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 9		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 10		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 11		50		25		85		75428737034162		63245275664384		3200		1400			Daphnia			60055		Ceriodaphnia			132086		Stenocypris			184149		Crangonyx			219432			Lampsilis			18783		Ceriodaphnia			21301		Hyalella			30879		Brachionus			46045
	Site 12		50		50		85		75428737034162		61388044609926		3100		1300			Daphnia			58291		Ceriodaphnia			128208		Stenocypris			178742		Crangonyx			212988			Lampsilis			18232		Ceriodaphnia			20676		Hyalella			29972		Brachionus			44693
	Site 13		50		75		85		75428737034162		60327020429132		3000		1300			Daphnia			57284		Ceriodaphnia			125992		Stenocypris			175652		Crangonyx			209307			Lampsilis			17917		Ceriodaphnia			20318		Hyalella			29454		Brachionus			43920
	Site 14		50		100		85		75428737034162		59585352130146		3000		1300			Daphnia			56580		Ceriodaphnia			124443		Stenocypris			173493		Crangonyx			206734			Lampsilis			17696		Ceriodaphnia			20068		Hyalella			29092		Salmo			41264
	Site 15		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 16		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 17		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 18		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 19		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 20		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370

Multiple Bioavailability Modeling Approaches Underwent External Peer Review

17

bull Biotic Ligand Modelsbull Full aluminum BLM (2016 model version) ndash Temperature pH

Dissolved organic carbon (DOC) Major cations (calcium [Ca] magnesium [Mg] sodium [Na] and potassium [K]) Major anions (sulfate [SO4] and chloride [Cl]) Alkalinity and Sulfide

bull Simplified aluminum BLM - pH hardness DOC temperature

bull Multilinear Regression Equationsbull pH and Hardness

Accounting for Effects of Water Chemistry on Bioavailability and Toxicity

bull Water quality criteria can be modified quantitatively when enough data are available to demonstrate that those conditions similarly affect the results with a variety of speciesndash High quality data in the draft aluminum document demonstrated

that pH DOC and hardness affected a variety of species in a similar way when exposed to aluminum concentrations

bull Recent publications by Cardwell et al (2017) and Gensemer et al (2017) summarized short-term aluminum chronic toxicity data across a range of DOC pH and hardness values for three species

18

Multiple Linear Regression (MLR) Models to Account for Effects of Water Chemistry

bull MLR models were developed for each species (DeForest et al 2017)ndash 119862119862 119889119889119906119906119887119887119894119894119886119886 11986411986411986211986220=119890119890[minus41026+[0525timesln(119863119863119874119874119862119862)]+[2201timesln(ℎ119886119886119903119903119889119889)]+(11282times119901119901119867119867)minus(0633times1199011199011198671198672)minus[0264times119901119901119867119867ln(ℎ119886119886119903119903119889119889)]]

ndash 119875119875 119901119901119903119903119900119900119898119898119890119890119897119897119886119886119904119904 11986411986411986211986220=119890119890[minus14029+[0503timesln(119863119863119874119874119862119862)]+[3443timesln(ℎ119886119886119903119903119889119889)]+(3131times119901119901119867119867)minus[0494times119901119901119867119867ln(ℎ119886119886119903119903119889119889)]]

bull These models were applied to both the acute and chronic aluminum toxicity datandash The C dubia model was applied to invertebrate datandash The P promelas model was applied to vertebrate data

19

III Normalize toxicity data in species sensitivity distribution to a set of water

quality conditionsbull Just like single parameter hardness models the MLR

models were used to normalize the aluminum toxicity data to a single set of water quality conditionsndash As an illustration for one set of calculations the draft

aluminum AWQC set these conditions to pH 7 hardness of 100 mgL and DOC of 1 mgL

bull This allows for comparisons to be made between species based on their sensitivity to aluminumndash Apples to apples

20

IV Construct sensitivity distribution to determine criteria at set conditions

21

Figure 8 Ranked Summary of Total Aluminum Genus Mean Acute Values (GMAVs) - Freshwater at pH 7 Hardness of 100 mgL and DOC of 1 mgL

IV Construct sensitivity distribution to determine criteria at set conditions

22

Figure 10 Ranked Summary of Total Aluminum Genus Mean Chronic Values (GMCVs) ndash Freshwater Supplemented with Other Data to Fulfill Missing MDRs at pH 7 Hardness of 100 mgL and DOC of 1 mgL

Aluminum Criteria Calculatorbull The relative ranking of genera in the sensitivity

distribution to which the toxicity data are normalized will change based on the set of water chemistry conditions (ie pH hardness and DOC)

bull To make calculating aluminum criteria easier EPA developed an Aluminum Criteria Calculatorndash Automated so that user only needs to enter water chemistry

conditions of interestndash Allows quick calculations for a variety of conditionsndash Limits calculations to the recommended conditions for which

the MLR models were developed were specified for hardness and DOC

bull No extrapolation beyond hardness and DOC empirical data bounds

23

Aluminum Criteria Calculator SD Example

24

pH=7 Hardness=100 DOC=1 pH=6 Hardness=25 DOC=1

Chronic Ranked GMCVRank GMCV Genus

12 17098 Aeolosoma Invert11 gt 9746 Rana Amphib - Other Data10 5908 Chironomus Invert

9 4877 Lymnaea Mollusk8 2555 Brachionus Invert7 2488 Pimephales Fish6 1713 Hyalella Invert5 1182 Ceriodaphnia Invert4 1102 Danio Fish3 1042 Lampsilis Mollusk2 591 Salvelinus Fish1 509 Salmo Fish

FCV = 3940 microgL total aluminum

Chronic Ranked GMCVRank GMCV Genus

12 gt 2131 Rana Amphib - Other Data11 1710 Aeolosoma Invert10 591 Chironomus Invert

9 544 Pimephales Fish8 488 Lymnaea Mollusk7 255 Brachionus Invert6 241 Danio Fish5 171 Hyalella Invert4 129 Salvelinus Fish3 118 Ceriodaphnia Invert2 111 Salmo Fish1 104 Lampsilis Mollusk

FCV = 987 microgL total aluminum

Multiple Scenarios

Run Scenarios

Summary Sheet

Acute Dataset

Chronic Dataset

Multiple Scenarios

Run Scenarios

Summary Sheet

Acute Dataset

Chronic Dataset

Boundaries of the Criteria Outputsbull MLR models were developed using data that

encompassndash DOC range of 008-5 mgLndash Hardness range of 98-127 mgL (as CaCO3)ndash pH range of 6-81

bull MLR criteria outputs are bounded atndash Maximum of 150 mgL total hardness (as CaCO3)

ndash DOC of 50 mgLndash pH range of the model is 50 to 90

25

Aluminum Criteria Look-Up Tables

26

bull EPA also provided criteria look-up tables in Appendix K

Har

dnes

s

CCC(microgL total aluminum)

(DOC=05 mgL)

pH 50 pH 55 pH 60 pH 65 pH 70 pH 75 pH 80 pH 85 pH 90

25 23 a 14 b 69 c 160 f 350 f 620 i 540 b 410 a 230 a

50 43 a 24 b 100 d 170 f 310 f 630 f 670 j 400 a 200 a

75 61 a 33 b 130 e 180 f 290 f 530 f 730 i 390 a 190 a

100 78 a 41 b 150 e 190 f 280 g 460 f 770 c 390 a 180 a

150 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

200 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

250 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

300 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

350 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

400 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

Aluminum Criteria Look-Up Tables

27

bull EPA also provided criteria look-up tables in Appendix K

Har

dnes

s

CCC(microgL total aluminum)

(DOC=10 mgL)

pH 50 pH 55 pH 60 pH 65 pH 70 pH 75 pH 80 pH 85 pH 90

25 33 a 21 b 99 c 220 f 490 f 900 i 780 b 590 a 330 a

50 61 a 35 b 150 e 250 f 440 f 890 f 960 j 580 a 290 a

75 88 a 47 b 180 e 260 f 410 f 740 f 1100 i 570 a 270 a

100 11 a 59 b 210 e 270 f 390 g 650 f 1100 c 560 b 260 a

150 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

200 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

250 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

300 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

350 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

400 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

Aluminum Criteria Look-Up Tables

28

bull EPA also provided criteria look-up tables in Appendix K

Har

dnes

s

CCC(microgL total aluminum)

(DOC=25 mgL)

pH 50 pH 55 pH 60 pH 65 pH 70 pH 75 pH 80 pH 85 pH 90

25 54 a 34 b 160 c 350 f 770 f 1500 i 1300 b 960 a 540 a

50 99 a 57 b 230 e 390 f 690 f 1400 f 1600 j 930 a 470 a

75 14 a 77 b 290 e 410 f 640 f 1200 f 1700 i 920 a 440 a

100 18 a 96 b 330 e 420 f 620 g 1000 f 1800 d 920 b 420 a

150 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

200 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

250 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

300 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

350 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

400 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

Aluminum Criteria Look-Up Tables

29

bull EPA also provided criteria look-up tables in Appendix K

Har

dnes

s

CCC(microgL total aluminum)

(DOC=50 mgL)

pH 50 pH 55 pH 60 pH 65 pH 70 pH 75 pH 80 pH 85 pH 90

25 77 a 49 b 230 c 500 f 1100 f 2100 c 1900 b 1400 a 770 a

50 14 a 82 b 330 e 540 f 970 f 2000 f 2200 j 1300 a 680 a

75 20 a 110 b 410 e 570 f 900 f 1600 f 2500 i 1300 a 640 a

100 26 a 140 b 460 f 590 f 880 g 1400 f 2500 d 1300 b 600 a

150 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

200 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

250 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

300 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

350 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

400 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

Status

30

bull Draft Document Released to the Federal Register on 72817ndash Public comment period of 60 days ndash Due Date for Comments is 92617

bull Revisions will be made considering public commentsbull Subsequent EPA intra-agency review of final criteria

document will then be conducted bull Publication of final criteria document planned for Late Fall

2017

Federal Register Webpagehttpswwwfederalregistergovdocuments201

707282017-15968request-for-scientific-views-draft-updated-aquatic-life-ambient-water-quality-criteria-for-aluminum

Aluminum Criteria Webpagehttpswwwepagovwqc2017-draft-aquatic-life-

criteria-aluminum-freshwater-documents

31

	Appendix C Acceptable Chronic Toxicity Data of Aluminum to Freshwater Aquatic Animals
									MLR equation
	Species		Hardness (mgL as CaCO3)		pH		DOC(mgL)		EC20 Endpoint			EC20(microgL)			Normalized Conc- EC20(microgL)			SMCV-EC20(microgL)		GMCV-EC20(microgL)			Genus			DOC Notes		Dilution water
	Oligochaete Aeolosoma sp		48		6		025		Reproduction (population size)			1235			1710			1710		1710			Aeolosoma		Invert		Author reported half the detection limit
	Rotifer Brachionus calyciflorus		100		62		025		Reproduction (population size)			431			255			255		255			Brachionus		Invert		Author reported half the detection limit
	Great pond snail Lymnaea stagnalis		117		61		025		Biomass			746			488			488		488			Lymnaea		Mollusk		Author reported half the detection limit
	Fatmucket Lampsilis siliquoidea		1055		604		040		Biomass			169			104			104		104			Lampsilis		Mollusk		Author reported
	Cladoceran Ceriodaphnia dubia		50		715		11		Reproduction - youngstarting adult			1780			190						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		26		73		05		Reproduction - youngfemale			1557			272						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		46		75		05		Reproduction - youngfemale			8087			110						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		96		79		05		Reproduction - youngfemale			6519			80						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		194		81		05		Reproduction - youngfemale			6836			89						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		255		703		05		Reproduction (youngfemale)			250			56						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		122		713		05		Reproduction (youngfemale)			860			106						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		25		798		05		Reproduction (youngfemale)			700			101						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		61		803		05		Reproduction (youngfemale)			1010			136						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		122		810		05		Reproduction (youngfemale)			870			116						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		255		633		05		Reproduction (youngfemale)			260			176						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		121		636		05		Reproduction (youngfemale)			390			110			118		118			Ceriodaphnia		Invert		Author reported
	Amphipod Hyalella azteca		95		61		051		Biomass (28 d)			1993			101						Hyalella			Author reported
	Amphipod Hyalella azteca		106		604		033		Biomass (28 d)			425			289			171		171			Hyalella		Invert		Author reported
	Midge Chironomus riparius		118		558		18		Adult midge emergence			2955			119						Chironomus			Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Midge Chironomus riparius		119		505		18		Adult midge emergence			8442			2258						Chironomus			Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Midge Chironomus riparius		91		66		051		Reproduction ( of eggscase)			3387			766			591		591			Chironomus		Invert		Author reported
	Atlantic salmon Salmo salar		127		57		18		Biomass			6156			1112			1112		1112			Salmo		Fish		Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Brook trout Salvelinus fontinalis		123		655		19		Biomass			1644			59						Salvelinus			Author reported		wellRO mix
	Brook trout Salvelinus fontinalis		128		565		18		Biomass			1435			283			1293		1293			Salvelinus		Fish		Author reported		wellRO mix
	Fathead minnow Pimephales promelas		220		770		16		Biomass			6194			781						Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		well water
	Fathead minnow Pimephales promelas		96		620		025		Fry survival			4286			379			544		544			Pimephales		Fish		Author reported half the detection limit
	Zebrafish Danio rerio		83		61		025		Biomass			2344			241			241		241			Danio		Fish		Author reported half the detection limit
	Wood frog Rana sylvatica		115		469		16		MATC-survival		gt		2000		gt		2131		gt		2131		2131		gt		Rana		Amphib - Other Data		Value is from 2007 FW Cu AWQC Appendix C recommendation		aged tap water

	Appendix A Acceptable Acute Toxicity Data of Aluminum to Freshwater Aquatic Animals
	Species		Methoda		Hardness (mgL as CaCO3)		pH		DOC(mgL)			LC50 or EC50 Conc (microgL)		Reference			Normalized Conc - LC50(microgL)			Species Mean Acute Value(microgL)		Genus Mean Acute Value(microgL)			Genus			DOC Note		Dilution water
	Snail Physa sp		S M T		474		659		11		gt		23400		Call 1984 Call et al 1984		gt		4843			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		755		11			30600		Call 1984 Call et al 1984			2699			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		817		11		gt		24700		Call 1984 Call et al 1984		gt		2404			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		746		11			55500		Call 1984 Call et al 1984			5034			3546		3546			Physa		Mollusk		Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Fatmucket Lampsilis siliquoidea		F M T		1060		612		048		gt		6302		Wang et al 2016 Wang et al 2017		gt		2983		gt		2983		2983		gt		Lampsilis		Mollusk		Author reported
	Cladoceran Ceriodaphnia dubia		S M A		500		742		11			1900		McCauley et al 1986			173			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		S M A		505		786		11			1500		McCauley et al 1986			129			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		UW-S Lake Superior lab water
	Cladoceran Ceriodaphnia dubia		S M A		500		813		11			2560		McCauley et al 1986			243			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		R M T		26		75		05			720		ENSR 1992d			111			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		R M T		46		76		05			1880		ENSR 1992d			250			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		R M T		96		78		05			2450		ENSR 1992d			289			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		S U NR		90		715		05			3727		Fort and Stover 1995			500			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U NR		90		715		05			5673		Fort and Stover 1995			761			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U NR		89		76		05			2880		Soucek et al 2001			336			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		601		05			7112		European Al Association 2009			169			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		605		2			6865		European Al Association 2009			709			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		609		4			15581		European Al Association 2009			1007			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		601		05			681		European Al Association 2009			162			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		603		05			1630		European Al Association 2009			367			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		597		05			1785		European Al Association 2009			473			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		592		05			1410		European Al Association 2009			429			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		699		05		gt		1300		European Al Association 2009		gt		419			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		785		05		gt		5000		European Al Association 2009		gt		796			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		68		2		gt		10000		European Al Association 2009		gt		2076			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		782		2		gt		15000		European Al Association 2009		gt		1163			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		677		4		gt		10000		European Al Association 2009		gt		1516			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		766		4		gt		15000		European Al Association 2009		gt		861			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		79		05		gt		2000		European Al Association 2009		gt		315			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		789		05		gt		2000		European Al Association 2009		gt		316			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		604		05			1108		European Al Association 2009			85			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		598		2			11371		European Al Association 2009			482			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		573		4			80467		European Al Association 2009			4357			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		671		05		gt		10000		European Al Association 2009		gt		2379			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		783		05		gt		5000		European Al Association 2009		gt		634			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		679		2		gt		10000		European Al Association 2009		gt		1039			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		767		2		gt		15000		European Al Association 2009		gt		908			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		668		4		gt		15000		European Al Association 2009		gt		1246			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		762		4		gt		15000		European Al Association 2009		gt		633			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		606		2			33868		European Al Association 2009			792			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		56		4			104842		European Al Association 2009			4880			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		693		05		gt		5000		European Al Association 2009		gt		713			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		120		788		05		gt		5000		European Al Association 2009		gt		590			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		120		676		2		gt		15000		European Al Association 2009		gt		1212			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		771		2		gt		15000		European Al Association 2009		gt		810			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		66		4		gt		15000		European Al Association 2009		gt		1014			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		76		4		gt		15000		European Al Association 2009		gt		555			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			11971		European Al Association 2010			270			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			27478		European Al Association 2010			619			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			11998		European Al Association 2010			270			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		607		05			92495		European Al Association 2010			188			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		609		05			31337		European Al Association 2010			604			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		61		05			33235		European Al Association 2010			624			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		708		05		gt		8864		European Al Association 2010		gt		253			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		779		05		gt		42783		European Al Association 2010		gt		693			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		753		05			13204		European Al Association 2010			24			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		600		601		05			46326		European Al Association 2010			380			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		600		599		05		gt		8590		European Al Association 2010		gt		737			509				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia reticulata		F M T		451		60		11			304		Shephard 1983			201			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia reticulata		F M T		40		55		11			362		Shephard 1983			5531			1054		733			Ceriodaphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake SuperiorDI Mix
	Cladoceran Daphnia magna		S U NR		453		774		11			3900		Biesinger and Christensen 1972			338			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Daphnia magna		S M T		220		76		16			38200		Kimball 1978			2030			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Well water
	Cladoceran Daphnia magna		S U T		451		725		11			2800		Shephard 1983			287			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Daphnia magna		S U T		168		599		05			5000		European Al Association 2009		gt		227			-				Daphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Daphnia magna		S U T		168		792		05			7950		European Al Association 2009			92			333		333			Daphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Ostracod Stenocypris major		R M T		1563		651		16			310196		Shuhaimi-Othman et al 2011a			1022			1022		1022			Stenocypris		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (Malaysia)
	Amphipod Crangonyx pseudogracilis		R U T		50		675		16			9190		Martin and Holdich 1986			1217			1217		1217			Crangonyx		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (UK)
	Amphipod Hyalella azteca		F M T		105		613		048		gt		5997		Wang et al 2016 Wang et al 2017		gt		2800		gt		2800		2800		gt		Hyalella		Invert		Author reported
	Stonefly Acroneuria sp		S M T		474		746		11		gt		22600		Call et al 1984		gt		2050		gt		2050		2050		gt		Acroneuria		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Midge Chironomus plumosus		S U T		80		70		16			30000		Fargasova 2001			2536			2536		2536			Chironomus		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (Slovakia)
	Midge Paratanytarsus dissimilis		S M T		1743		728		28		gt		77700		Lamb and Bailey 1981 1983		gt		6232		gt		6232		6232		gt		Paratanytarsus		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Liberty Lake Washington
	Rainbow trout Oncorhynchus mykiss		F M T		274		758		05		gt		9840		Gundersen et al 1994		gt		1256			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		452		762		05		gt		8070		Gundersen et al 1994		gt		1140			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		895		758		05		gt		8160		Gundersen et al 1994		gt		1489			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		1304		758		05		gt		8200		Gundersen et al 1994		gt		1676			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		232		825		05			6170		Gundersen et al 1994			260			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		350		826		05			6170		Gundersen et al 1994			333			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		836		829		05			7670		Gundersen et al 1994			701			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		1158		830		05			6930		Gundersen et al 1994			777			801		801			Oncorhynchus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Atlantic salmon Salmo salar		S U T		68		55		05			584		Hamilton and Haines 1995			4602			-				Salmo			Value is from 2007 FW Cu AWQC Appendix C recommendation		Soft recon
	Atlantic salmon Salmo salar		S U T		68		65		05			599		Hamilton and Haines 1995			531			1564		1564			Salmo		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Soft recon
	Brook trout Salvelinus fontinalis		S U T		40		56		16			6530		Tandjung 1982			6947			-				Salvelinus			Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Brook trout Salvelinus fontinalis		S U T		18		56		16			3400		Tandjung 1982			6209			-				Salvelinus			Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Brook trout Salvelinus fontinalis		S U T		2		56		16			370		Tandjung 1982			2988			5051		5051			Salvelinus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Green sunfish Lepomis cyanellus		S M T		474		755		11		gt		50000		Call et al 1984		gt		5255		gt		5255		5255		gt		Lepomis		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Rio Grande silvery minnow Hybognathus amarus		R M T		140		81		05		gt		59100		Buhl 2002		gt		8620		gt		8620		8620		gt		Hybognathus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon water
	Fathead minnow Pimephales promelas		S M T		474		761		11		gt		48200		Call et al 1984		gt		4707			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Fathead minnow Pimephales promelas		S M T		474		805		11		gt		49800		Call et al 1984		gt		2837			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Fathead minnow Pimephales promelas		R M T		26		78		05			1160		ENSR 1992c			104			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		46		76		05			8180		ENSR 1992c			1191			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		96		81		05			20300		ENSR 1992c			2398			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		194		81		05			44800		ENSR 1992c			7839			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		140		81		05		gt		59100		Buhl 2002		gt		8620			2224		2224			Pimephales		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon water
	Smallmouth bass Micropterus dolomieui		S M T		1245		505		16			130		Kane and Rabeni 1987			859			859		859			Micropterus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Green tree frog Hyla cinerea		R M T		455		549		05		gt		4052		Jung and Jagoe 1995		gt		4377		gt		4377		4377		gt		Hyla		Amphibian		Value is from 2007 FW Cu AWQC Appendix C recommendation		artificial soft recon

	Normalization Chemistry
	pH		6					FAV		360
	Hardness		25					CMC		180
	DOC		1					CCC		99
	Acute Ranked GMAV											Chronic Ranked GMCV
	Rank			GMAV		Genus								Rank			GMCV		Genus
	18		gt		8620		Hybognathus		Fish							12		gt		2131		Rana		Amphib - Other Data
	17		gt		6232		Paratanytarsus		Invert							11			1710		Aeolosoma		Invert
	16		gt		5255		Lepomis		Fish							10			591		Chironomus		Invert
	15			5051		Salvelinus		Fish							9			544		Pimephales		Fish
	14		gt		4377		Hyla		Amphibian							8			488		Lymnaea		Mollusk
	13			3546		Physa		Mollusk							7			255		Brachionus		Invert
	12		gt		2983		Lampsilis		Mollusk							6			241		Danio		Fish
	11		gt		2800		Hyalella		Invert							5			171		Hyalella		Invert
	10			2536		Chironomus		Invert							4			129		Salvelinus		Fish
	9			2224		Pimephales		Fish							3			118		Ceriodaphnia		Invert
	8		gt		2050		Acroneuria		Invert							2			111		Salmo		Fish
	7			1564		Salmo		Fish							1			104		Lampsilis		Mollusk
	6			1217		Crangonyx		Invert
	5			1022		Stenocypris		Invert
	4			859		Micropterus		Fish
	3			801		Oncorhynchus		Fish
	2			733		Ceriodaphnia		Invert
	1			333		Daphnia		Invert
		N		Rank		Genus		GMAV		ln(GMAV)		ln(GMAV)2		P=R(N+1)		sqrt(P)				N		Rank		Genus		GMCV		ln(GMCV)		ln(GMCV)2		P=R(N+1)		sqrt(P)
		18		4		Micropterus		859		676		4564		0211		0459				12		4		Salvelinus		129		486		2364		0308		0555
			3		Oncorhynchus		801		669		4469		0158		0397					3		Ceriodaphnia		118		477		2277		0231		0480
			2		Ceriodaphnia		733		660		4352		0105		0324					2		Salmo		111		471		2220		0154		0392
			1		Daphnia		333		581		3374		0053		0229					1		Lampsilis		104		465		2159		0077		0277
			Sum				2585		1676		0526		141					Sum				1899		902		0769		170
			S2 =		1987										S2 =		059
			L =		4890										L =		4420
			A =		5887										A =		4592
			FAV =		360										FCV =		987
			CMC =		180

				All concentrations reported are microgL total Aluminum
				lt-----Click Button after entering data
	ENTER DATA HERE									ERRORNUM		ACUTE														CHRONIC
										Rank 1				Rank 2				Rank 3				Rank 4					Rank 1				Rank 2				Rank 3				Rank 4
	SiteName		DOC(mgL)		Hardness(mgL as CaCO3)		pH			FAV		CMC		CCC			Genus		GMAV			Genus		GMAV			Genus		GMAV			Genus		GMAV				Genus		GMCV			Genus		GMCV			Genus		GMCV			Genus		GMCV
	Site 1		50		25		9		75428737034162		35229324666008		1800		770			Daphnia			33452		Ceriodaphnia			73576		Stenocypris			102576		Crangonyx			122230			Lampsilis			10463		Ceriodaphnia			11865		Hyalella			17200		Brachionus			25648
	Site 2		50		50		9		75428737034162		31204991509063		1600		680			Daphnia			29631		Ceriodaphnia			65171		Stenocypris			90859		Crangonyx			108267			Lampsilis			9268		Ceriodaphnia			10510		Hyalella			15236		Brachionus			22718
	Site 3		50		75		9		75428737034162		29067527928199		1500		640			Daphnia			27601		Ceriodaphnia			60707		Stenocypris			84635		Crangonyx			100851			Lampsilis			8633		Ceriodaphnia			9790		Hyalella			14192		Brachionus			21162
	Site 4		50		100		9		75428737034162		27640367912592		1400		600			Daphnia			26246		Ceriodaphnia			57726		Stenocypris			80480		Crangonyx			95899			Lampsilis			8209		Ceriodaphnia			9309		Hyalella			13495		Brachionus			20123
	Site 5		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 6		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 7		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 8		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 9		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 10		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 11		50		25		85		75428737034162		63245275664384		3200		1400			Daphnia			60055		Ceriodaphnia			132086		Stenocypris			184149		Crangonyx			219432			Lampsilis			18783		Ceriodaphnia			21301		Hyalella			30879		Brachionus			46045
	Site 12		50		50		85		75428737034162		61388044609926		3100		1300			Daphnia			58291		Ceriodaphnia			128208		Stenocypris			178742		Crangonyx			212988			Lampsilis			18232		Ceriodaphnia			20676		Hyalella			29972		Brachionus			44693
	Site 13		50		75		85		75428737034162		60327020429132		3000		1300			Daphnia			57284		Ceriodaphnia			125992		Stenocypris			175652		Crangonyx			209307			Lampsilis			17917		Ceriodaphnia			20318		Hyalella			29454		Brachionus			43920
	Site 14		50		100		85		75428737034162		59585352130146		3000		1300			Daphnia			56580		Ceriodaphnia			124443		Stenocypris			173493		Crangonyx			206734			Lampsilis			17696		Ceriodaphnia			20068		Hyalella			29092		Salmo			41264
	Site 15		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 16		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 17		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 18		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 19		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 20		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370

	Appendix C Acceptable Chronic Toxicity Data of Aluminum to Freshwater Aquatic Animals
									MLR equation
	Species		Hardness (mgL as CaCO3)		pH		DOC(mgL)		EC20 Endpoint			EC20(microgL)			Normalized Conc- EC20(microgL)			SMCV-EC20(microgL)		GMCV-EC20(microgL)			Genus			DOC Notes		Dilution water
	Oligochaete Aeolosoma sp		48		6		025		Reproduction (population size)			1235			17098			17098		17098			Aeolosoma		Invert		Author reported half the detection limit
	Rotifer Brachionus calyciflorus		100		62		025		Reproduction (population size)			431			2555			2555		2555			Brachionus		Invert		Author reported half the detection limit
	Great pond snail Lymnaea stagnalis		117		61		025		Biomass			746			4877			4877		4877			Lymnaea		Mollusk		Author reported half the detection limit
	Fatmucket Lampsilis siliquoidea		1055		604		040		Biomass			169			1042			1042		1042			Lampsilis		Mollusk		Author reported
	Cladoceran Ceriodaphnia dubia		50		715		11		Reproduction - youngstarting adult			1780			1898						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		26		73		05		Reproduction - youngfemale			1557			2718						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		46		75		05		Reproduction - youngfemale			8087			1102						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		96		79		05		Reproduction - youngfemale			6519			796						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		194		81		05		Reproduction - youngfemale			6836			888						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		255		703		05		Reproduction (youngfemale)			250			563						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		122		713		05		Reproduction (youngfemale)			860			1061						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		25		798		05		Reproduction (youngfemale)			700			1006						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		61		803		05		Reproduction (youngfemale)			1010			1363						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		122		810		05		Reproduction (youngfemale)			870			1164						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		255		633		05		Reproduction (youngfemale)			260			1758						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		121		636		05		Reproduction (youngfemale)			390			1101			1182		1182			Ceriodaphnia		Invert		Author reported
	Amphipod Hyalella azteca		95		61		051		Biomass (28 d)			1993			1014						Hyalella			Author reported
	Amphipod Hyalella azteca		106		604		033		Biomass (28 d)			425			2895			1713		1713			Hyalella		Invert		Author reported
	Midge Chironomus riparius		118		558		18		Adult midge emergence			2955			1192						Chironomus			Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Midge Chironomus riparius		119		505		18		Adult midge emergence			8442			22578						Chironomus			Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Midge Chironomus riparius		91		66		051		Reproduction ( of eggscase)			3387			7664			5908		5908			Chironomus		Invert		Author reported
	Atlantic salmon Salmo salar		127		57		18		Biomass			6156			5085			5085		5085			Salmo		Fish		Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Brook trout Salvelinus fontinalis		123		655		19		Biomass			1644			270						Salvelinus			Author reported		wellRO mix
	Brook trout Salvelinus fontinalis		128		565		18		Biomass			1435			1295			5914		5914			Salvelinus		Fish		Author reported		wellRO mix
	Fathead minnow Pimephales promelas		220		770		16		Biomass			6194			3569						Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		well water
	Fathead minnow Pimephales promelas		96		620		025		Fry survival			4286			1734			2488		2488			Pimephales		Fish		Author reported half the detection limit
	Zebrafish Danio rerio		83		61		025		Biomass			2344			1102			1102		1102			Danio		Fish		Author reported half the detection limit
	Wood frog Rana sylvatica		115		469		16		MATC-survival		gt		2000		gt		9746		gt		9746		9746		gt		Rana		Amphib - Other Data		Value is from 2007 FW Cu AWQC Appendix C recommendation		aged tap water

	Appendix A Acceptable Acute Toxicity Data of Aluminum to Freshwater Aquatic Animals
	Species		Methoda		Hardness (mgL as CaCO3)		pH		DOC(mgL)			LC50 or EC50 Conc (microgL)		Reference			Normalized Conc - LC50(microgL)			Species Mean Acute Value(microgL)		Genus Mean Acute Value(microgL)			Genus			DOC Note		Dilution water
	Snail Physa sp		S M T		474		659		11		gt		23400		Call 1984 Call et al 1984		gt		48425			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		755		11			30600		Call 1984 Call et al 1984			26986			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		817		11		gt		24700		Call 1984 Call et al 1984		gt		24040			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		746		11			55500		Call 1984 Call et al 1984			50338			35462		35462			Physa		Mollusk		Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Fatmucket Lampsilis siliquoidea		F M T		1060		612		048		gt		6302		Wang et al 2016 Wang et al 2017		gt		29834		gt		29834		29834		gt		Lampsilis		Mollusk		Author reported
	Cladoceran Ceriodaphnia dubia		S M A		500		742		11			1900		McCauley et al 1986			1728			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		S M A		505		786		11			1500		McCauley et al 1986			1294			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		UW-S Lake Superior lab water
	Cladoceran Ceriodaphnia dubia		S M A		500		813		11			2560		McCauley et al 1986			2431			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		R M T		26		75		05			720		ENSR 1992d			1113			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		R M T		46		76		05			1880		ENSR 1992d			2495			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		R M T		96		78		05			2450		ENSR 1992d			2894			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		S U NR		90		715		05			3727		Fort and Stover 1995			5002			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U NR		90		715		05			5673		Fort and Stover 1995			7613			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U NR		89		76		05			2880		Soucek et al 2001			3362			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		601		05			7112		European Al Association 2009			1691			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		605		2			6865		European Al Association 2009			7088			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		609		4			15581		European Al Association 2009			10072			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		601		05			681		European Al Association 2009			1620			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		603		05			1630		European Al Association 2009			3674			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		597		05			1785		European Al Association 2009			4732			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		592		05			1410		European Al Association 2009			4295			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		699		05		gt		1300		European Al Association 2009		gt		4189			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		785		05		gt		5000		European Al Association 2009		gt		7960			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		68		2		gt		10000		European Al Association 2009		gt		20755			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		782		2		gt		15000		European Al Association 2009		gt		11626			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		677		4		gt		10000		European Al Association 2009		gt		15161			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		766		4		gt		15000		European Al Association 2009		gt		8608			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		79		05		gt		2000		European Al Association 2009		gt		3150			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		789		05		gt		2000		European Al Association 2009		gt		3156			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		604		05			1108		European Al Association 2009			850			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		598		2			11371		European Al Association 2009			4817			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		573		4			80467		European Al Association 2009			43570			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		671		05		gt		10000		European Al Association 2009		gt		23786			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		783		05		gt		5000		European Al Association 2009		gt		6344			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		679		2		gt		10000		European Al Association 2009		gt		10394			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		767		2		gt		15000		European Al Association 2009		gt		9082			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		668		4		gt		15000		European Al Association 2009		gt		12461			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		762		4		gt		15000		European Al Association 2009		gt		6332			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		606		2			33868		European Al Association 2009			7920			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		56		4			104842		European Al Association 2009			48800			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		693		05		gt		5000		European Al Association 2009		gt		7126			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		120		788		05		gt		5000		European Al Association 2009		gt		5898			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		120		676		2		gt		15000		European Al Association 2009		gt		12117			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		771		2		gt		15000		European Al Association 2009		gt		8096			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		66		4		gt		15000		European Al Association 2009		gt		10139			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		76		4		gt		15000		European Al Association 2009		gt		5545			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			11971		European Al Association 2010			2699			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			27478		European Al Association 2010			6194			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			11998		European Al Association 2010			2705			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		607		05			92495		European Al Association 2010			1876			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		609		05			31337		European Al Association 2010			6035			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		61		05			33235		European Al Association 2010			6238			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		708		05		gt		8864		European Al Association 2010		gt		2534			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		779		05		gt		42783		European Al Association 2010		gt		6930			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		753		05			13204		European Al Association 2010			244			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		600		601		05			46326		European Al Association 2010			3798			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		600		599		05		gt		8590		European Al Association 2010		gt		7366			5094				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia reticulata		F M T		451		60		11			304		Shephard 1983			2009			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia reticulata		F M T		40		55		11			362		Shephard 1983			55307			10542		7328			Ceriodaphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake SuperiorDI Mix
	Cladoceran Daphnia magna		S U NR		453		774		11			3900		Biesinger and Christensen 1972			3382			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Daphnia magna		S M T		220		76		16			38200		Kimball 1978			20303			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Well water
	Cladoceran Daphnia magna		S U T		451		725		11			2800		Shephard 1983			2868			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Daphnia magna		S U T		168		599		05			5000		European Al Association 2009		gt		2265			-				Daphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Daphnia magna		S U T		168		792		05			7950		European Al Association 2009			920			3332		3332			Daphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Ostracod Stenocypris major		R M T		1563		651		16			310196		Shuhaimi-Othman et al 2011a			10216			10216		10216			Stenocypris		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (Malaysia)
	Amphipod Crangonyx pseudogracilis		R U T		50		675		16			9190		Martin and Holdich 1986			12174			12174		12174			Crangonyx		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (UK)
	Amphipod Hyalella azteca		F M T		105		613		048		gt		5997		Wang et al 2016 Wang et al 2017		gt		28002		gt		28002		28002		gt		Hyalella		Invert		Author reported
	Stonefly Acroneuria sp		S M T		474		746		11		gt		22600		Call et al 1984		gt		20498		gt		20498		20498		gt		Acroneuria		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Midge Chironomus plumosus		S U T		80		70		16			30000		Fargasova 2001			25361			25361		25361			Chironomus		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (Slovakia)
	Midge Paratanytarsus dissimilis		S M T		1743		728		28		gt		77700		Lamb and Bailey 1981 1983		gt		62318		gt		62318		62318		gt		Paratanytarsus		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Liberty Lake Washington
	Rainbow trout Oncorhynchus mykiss		F M T		274		758		05		gt		9840		Gundersen et al 1994		gt		5745			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		452		762		05		gt		8070		Gundersen et al 1994		gt		5212			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		895		758		05		gt		8160		Gundersen et al 1994		gt		6807			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		1304		758		05		gt		8200		Gundersen et al 1994		gt		7662			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		232		825		05			6170		Gundersen et al 1994			1190			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		350		826		05			6170		Gundersen et al 1994			1523			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		836		829		05			7670		Gundersen et al 1994			3205			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		1158		830		05			6930		Gundersen et al 1994			3554			3661		3661			Oncorhynchus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Atlantic salmon Salmo salar		S U T		68		55		05			584		Hamilton and Haines 1995			21042			-				Salmo			Value is from 2007 FW Cu AWQC Appendix C recommendation		Soft recon
	Atlantic salmon Salmo salar		S U T		68		65		05			599		Hamilton and Haines 1995			2430			7151		7151			Salmo		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Soft recon
	Brook trout Salvelinus fontinalis		S U T		40		56		16			6530		Tandjung 1982			31767			-				Salvelinus			Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Brook trout Salvelinus fontinalis		S U T		18		56		16			3400		Tandjung 1982			28391			-				Salvelinus			Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Brook trout Salvelinus fontinalis		S U T		2		56		16			370		Tandjung 1982			13663			23097		23097			Salvelinus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Green sunfish Lepomis cyanellus		S M T		474		755		11		gt		50000		Call et al 1984		gt		24028		gt		24028		24028		gt		Lepomis		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Rio Grande silvery minnow Hybognathus amarus		R M T		140		81		05		gt		59100		Buhl 2002		gt		39414		gt		39414		39414		gt		Hybognathus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon water
	Fathead minnow Pimephales promelas		S M T		474		761		11		gt		48200		Call et al 1984		gt		21522			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Fathead minnow Pimephales promelas		S M T		474		805		11		gt		49800		Call et al 1984		gt		12972			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Fathead minnow Pimephales promelas		R M T		26		78		05			1160		ENSR 1992c			477			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		46		76		05			8180		ENSR 1992c			5446			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		96		81		05			20300		ENSR 1992c			10966			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		194		81		05			44800		ENSR 1992c			35847			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		140		81		05		gt		59100		Buhl 2002		gt		39414			10168		10168			Pimephales		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon water
	Smallmouth bass Micropterus dolomieui		S M T		1245		505		16			130		Kane and Rabeni 1987			3929			3929		3929			Micropterus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Green tree frog Hyla cinerea		R M T		455		549		05		gt		4052		Jung and Jagoe 1995		gt		20016		gt		20016		20016		gt		Hyla		Amphibian		Value is from 2007 FW Cu AWQC Appendix C recommendation		artificial soft recon

	Normalization Chemistry
	pH		7					FAV		2741
	Hardness		100					CMC		1400
	DOC		1					CCC		390
	Acute Ranked GMAV											Chronic Ranked GMCV
	Rank			GMAV		Genus								Rank			GMCV		Genus
	18		gt		62318		Paratanytarsus		Invert							12			17098		Aeolosoma		Invert
	17		gt		39414		Hybognathus		Fish							11		gt		9746		Rana		Amphib - Other Data
	16			35462		Physa		Mollusk							10			5908		Chironomus		Invert
	15		gt		29834		Lampsilis		Mollusk							9			4877		Lymnaea		Mollusk
	14		gt		28002		Hyalella		Invert							8			2555		Brachionus		Invert
	13			25361		Chironomus		Invert							7			2488		Pimephales		Fish
	12		gt		24028		Lepomis		Fish							6			1713		Hyalella		Invert
	11			23097		Salvelinus		Fish							5			1182		Ceriodaphnia		Invert
	10		gt		20498		Acroneuria		Invert							4			1102		Danio		Fish
	9		gt		20016		Hyla		Amphibian							3			1042		Lampsilis		Mollusk
	8			12174		Crangonyx		Invert							2			591		Salvelinus		Fish
	7			10216		Stenocypris		Invert							1			509		Salmo		Fish
	6			10168		Pimephales		Fish
	5			7328		Ceriodaphnia		Invert
	4			7151		Salmo		Fish
	3			3929		Micropterus		Fish
	2			3661		Oncorhynchus		Fish
	1			3332		Daphnia		Invert
		N		Rank		Genus		GMAV		ln(GMAV)		ln(GMAV)2		P=R(N+1)		sqrt(P)				N		Rank		Genus		GMCV		ln(GMCV)		ln(GMCV)2		P=R(N+1)		sqrt(P)
		18		4		Salmo		7151		887		7876		0211		0459				12		4		Danio		1102		700		4907		0308		0555
			3		Micropterus		3929		828		6849		0158		0397					3		Lampsilis		1042		695		4829		0231		0480
			2		Oncorhynchus		3661		821		6733		0105		0324					2		Salvelinus		591		638		4074		0154		0392
			1		Daphnia		3332		811		6579		0053		0229					1		Salmo		509		623		3883		0077		0277
			Sum				3347		2804		0526		141					Sum				2657		1769		0769		170
			S2 =		1223										S2 =		1080
			L =		7134										L =		5242
			A =		7916										A =		5976
			FAV =		2741										FCV =		3940
			CMC =		1371

				All concentrations reported are microgL total Aluminum
				lt-----Click Button after entering data
	ENTER DATA HERE									ERRORNUM		ACUTE														CHRONIC
										Rank 1				Rank 2				Rank 3				Rank 4					Rank 1				Rank 2				Rank 3				Rank 4
	SiteName		DOC(mgL)		Hardness(mgL as CaCO3)		pH			FAV		CMC		CCC			Genus		GMAV			Genus		GMAV			Genus		GMAV			Genus		GMAV				Genus		GMCV			Genus		GMCV			Genus		GMCV			Genus		GMCV
	Site 1		50		25		9		75428737034162		35229324666008		1800		770			Daphnia			33452		Ceriodaphnia			73576		Stenocypris			102576		Crangonyx			122230			Lampsilis			10463		Ceriodaphnia			11865		Hyalella			17200		Brachionus			25648
	Site 2		50		50		9		75428737034162		31204991509063		1600		680			Daphnia			29631		Ceriodaphnia			65171		Stenocypris			90859		Crangonyx			108267			Lampsilis			9268		Ceriodaphnia			10510		Hyalella			15236		Brachionus			22718
	Site 3		50		75		9		75428737034162		29067527928199		1500		640			Daphnia			27601		Ceriodaphnia			60707		Stenocypris			84635		Crangonyx			100851			Lampsilis			8633		Ceriodaphnia			9790		Hyalella			14192		Brachionus			21162
	Site 4		50		100		9		75428737034162		27640367912592		1400		600			Daphnia			26246		Ceriodaphnia			57726		Stenocypris			80480		Crangonyx			95899			Lampsilis			8209		Ceriodaphnia			9309		Hyalella			13495		Brachionus			20123
	Site 5		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 6		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 7		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 8		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 9		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 10		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 11		50		25		85		75428737034162		63245275664384		3200		1400			Daphnia			60055		Ceriodaphnia			132086		Stenocypris			184149		Crangonyx			219432			Lampsilis			18783		Ceriodaphnia			21301		Hyalella			30879		Brachionus			46045
	Site 12		50		50		85		75428737034162		61388044609926		3100		1300			Daphnia			58291		Ceriodaphnia			128208		Stenocypris			178742		Crangonyx			212988			Lampsilis			18232		Ceriodaphnia			20676		Hyalella			29972		Brachionus			44693
	Site 13		50		75		85		75428737034162		60327020429132		3000		1300			Daphnia			57284		Ceriodaphnia			125992		Stenocypris			175652		Crangonyx			209307			Lampsilis			17917		Ceriodaphnia			20318		Hyalella			29454		Brachionus			43920
	Site 14		50		100		85		75428737034162		59585352130146		3000		1300			Daphnia			56580		Ceriodaphnia			124443		Stenocypris			173493		Crangonyx			206734			Lampsilis			17696		Ceriodaphnia			20068		Hyalella			29092		Salmo			41264
	Site 15		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 16		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 17		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 18		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 19		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 20		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370

Accounting for Effects of Water Chemistry on Bioavailability and Toxicity

bull Water quality criteria can be modified quantitatively when enough data are available to demonstrate that those conditions similarly affect the results with a variety of speciesndash High quality data in the draft aluminum document demonstrated

that pH DOC and hardness affected a variety of species in a similar way when exposed to aluminum concentrations

bull Recent publications by Cardwell et al (2017) and Gensemer et al (2017) summarized short-term aluminum chronic toxicity data across a range of DOC pH and hardness values for three species

18

Multiple Linear Regression (MLR) Models to Account for Effects of Water Chemistry

bull MLR models were developed for each species (DeForest et al 2017)ndash 119862119862 119889119889119906119906119887119887119894119894119886119886 11986411986411986211986220=119890119890[minus41026+[0525timesln(119863119863119874119874119862119862)]+[2201timesln(ℎ119886119886119903119903119889119889)]+(11282times119901119901119867119867)minus(0633times1199011199011198671198672)minus[0264times119901119901119867119867ln(ℎ119886119886119903119903119889119889)]]

ndash 119875119875 119901119901119903119903119900119900119898119898119890119890119897119897119886119886119904119904 11986411986411986211986220=119890119890[minus14029+[0503timesln(119863119863119874119874119862119862)]+[3443timesln(ℎ119886119886119903119903119889119889)]+(3131times119901119901119867119867)minus[0494times119901119901119867119867ln(ℎ119886119886119903119903119889119889)]]

bull These models were applied to both the acute and chronic aluminum toxicity datandash The C dubia model was applied to invertebrate datandash The P promelas model was applied to vertebrate data

19

III Normalize toxicity data in species sensitivity distribution to a set of water

quality conditionsbull Just like single parameter hardness models the MLR

models were used to normalize the aluminum toxicity data to a single set of water quality conditionsndash As an illustration for one set of calculations the draft

aluminum AWQC set these conditions to pH 7 hardness of 100 mgL and DOC of 1 mgL

bull This allows for comparisons to be made between species based on their sensitivity to aluminumndash Apples to apples

20

IV Construct sensitivity distribution to determine criteria at set conditions

21

Figure 8 Ranked Summary of Total Aluminum Genus Mean Acute Values (GMAVs) - Freshwater at pH 7 Hardness of 100 mgL and DOC of 1 mgL

IV Construct sensitivity distribution to determine criteria at set conditions

22

Figure 10 Ranked Summary of Total Aluminum Genus Mean Chronic Values (GMCVs) ndash Freshwater Supplemented with Other Data to Fulfill Missing MDRs at pH 7 Hardness of 100 mgL and DOC of 1 mgL

Aluminum Criteria Calculatorbull The relative ranking of genera in the sensitivity

distribution to which the toxicity data are normalized will change based on the set of water chemistry conditions (ie pH hardness and DOC)

bull To make calculating aluminum criteria easier EPA developed an Aluminum Criteria Calculatorndash Automated so that user only needs to enter water chemistry

conditions of interestndash Allows quick calculations for a variety of conditionsndash Limits calculations to the recommended conditions for which

the MLR models were developed were specified for hardness and DOC

bull No extrapolation beyond hardness and DOC empirical data bounds

23

Aluminum Criteria Calculator SD Example

24

pH=7 Hardness=100 DOC=1 pH=6 Hardness=25 DOC=1

Chronic Ranked GMCVRank GMCV Genus

12 17098 Aeolosoma Invert11 gt 9746 Rana Amphib - Other Data10 5908 Chironomus Invert

9 4877 Lymnaea Mollusk8 2555 Brachionus Invert7 2488 Pimephales Fish6 1713 Hyalella Invert5 1182 Ceriodaphnia Invert4 1102 Danio Fish3 1042 Lampsilis Mollusk2 591 Salvelinus Fish1 509 Salmo Fish

FCV = 3940 microgL total aluminum

Chronic Ranked GMCVRank GMCV Genus

12 gt 2131 Rana Amphib - Other Data11 1710 Aeolosoma Invert10 591 Chironomus Invert

9 544 Pimephales Fish8 488 Lymnaea Mollusk7 255 Brachionus Invert6 241 Danio Fish5 171 Hyalella Invert4 129 Salvelinus Fish3 118 Ceriodaphnia Invert2 111 Salmo Fish1 104 Lampsilis Mollusk

FCV = 987 microgL total aluminum

Multiple Scenarios

Run Scenarios

Summary Sheet

Acute Dataset

Chronic Dataset

Multiple Scenarios

Run Scenarios

Summary Sheet

Acute Dataset

Chronic Dataset

Boundaries of the Criteria Outputsbull MLR models were developed using data that

encompassndash DOC range of 008-5 mgLndash Hardness range of 98-127 mgL (as CaCO3)ndash pH range of 6-81

bull MLR criteria outputs are bounded atndash Maximum of 150 mgL total hardness (as CaCO3)

ndash DOC of 50 mgLndash pH range of the model is 50 to 90

25

Aluminum Criteria Look-Up Tables

26

bull EPA also provided criteria look-up tables in Appendix K

Har

dnes

s

CCC(microgL total aluminum)

(DOC=05 mgL)

pH 50 pH 55 pH 60 pH 65 pH 70 pH 75 pH 80 pH 85 pH 90

25 23 a 14 b 69 c 160 f 350 f 620 i 540 b 410 a 230 a

50 43 a 24 b 100 d 170 f 310 f 630 f 670 j 400 a 200 a

75 61 a 33 b 130 e 180 f 290 f 530 f 730 i 390 a 190 a

100 78 a 41 b 150 e 190 f 280 g 460 f 770 c 390 a 180 a

150 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

200 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

250 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

300 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

350 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

400 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

Aluminum Criteria Look-Up Tables

27

bull EPA also provided criteria look-up tables in Appendix K

Har

dnes

s

CCC(microgL total aluminum)

(DOC=10 mgL)

pH 50 pH 55 pH 60 pH 65 pH 70 pH 75 pH 80 pH 85 pH 90

25 33 a 21 b 99 c 220 f 490 f 900 i 780 b 590 a 330 a

50 61 a 35 b 150 e 250 f 440 f 890 f 960 j 580 a 290 a

75 88 a 47 b 180 e 260 f 410 f 740 f 1100 i 570 a 270 a

100 11 a 59 b 210 e 270 f 390 g 650 f 1100 c 560 b 260 a

150 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

200 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

250 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

300 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

350 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

400 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

Aluminum Criteria Look-Up Tables

28

bull EPA also provided criteria look-up tables in Appendix K

Har

dnes

s

CCC(microgL total aluminum)

(DOC=25 mgL)

pH 50 pH 55 pH 60 pH 65 pH 70 pH 75 pH 80 pH 85 pH 90

25 54 a 34 b 160 c 350 f 770 f 1500 i 1300 b 960 a 540 a

50 99 a 57 b 230 e 390 f 690 f 1400 f 1600 j 930 a 470 a

75 14 a 77 b 290 e 410 f 640 f 1200 f 1700 i 920 a 440 a

100 18 a 96 b 330 e 420 f 620 g 1000 f 1800 d 920 b 420 a

150 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

200 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

250 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

300 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

350 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

400 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

Aluminum Criteria Look-Up Tables

29

bull EPA also provided criteria look-up tables in Appendix K

Har

dnes

s

CCC(microgL total aluminum)

(DOC=50 mgL)

pH 50 pH 55 pH 60 pH 65 pH 70 pH 75 pH 80 pH 85 pH 90

25 77 a 49 b 230 c 500 f 1100 f 2100 c 1900 b 1400 a 770 a

50 14 a 82 b 330 e 540 f 970 f 2000 f 2200 j 1300 a 680 a

75 20 a 110 b 410 e 570 f 900 f 1600 f 2500 i 1300 a 640 a

100 26 a 140 b 460 f 590 f 880 g 1400 f 2500 d 1300 b 600 a

150 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

200 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

250 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

300 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

350 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

400 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

Status

30

bull Draft Document Released to the Federal Register on 72817ndash Public comment period of 60 days ndash Due Date for Comments is 92617

bull Revisions will be made considering public commentsbull Subsequent EPA intra-agency review of final criteria

document will then be conducted bull Publication of final criteria document planned for Late Fall

2017

Federal Register Webpagehttpswwwfederalregistergovdocuments201

707282017-15968request-for-scientific-views-draft-updated-aquatic-life-ambient-water-quality-criteria-for-aluminum

Aluminum Criteria Webpagehttpswwwepagovwqc2017-draft-aquatic-life-

criteria-aluminum-freshwater-documents

31

	Appendix C Acceptable Chronic Toxicity Data of Aluminum to Freshwater Aquatic Animals
									MLR equation
	Species		Hardness (mgL as CaCO3)		pH		DOC(mgL)		EC20 Endpoint			EC20(microgL)			Normalized Conc- EC20(microgL)			SMCV-EC20(microgL)		GMCV-EC20(microgL)			Genus			DOC Notes		Dilution water
	Oligochaete Aeolosoma sp		48		6		025		Reproduction (population size)			1235			1710			1710		1710			Aeolosoma		Invert		Author reported half the detection limit
	Rotifer Brachionus calyciflorus		100		62		025		Reproduction (population size)			431			255			255		255			Brachionus		Invert		Author reported half the detection limit
	Great pond snail Lymnaea stagnalis		117		61		025		Biomass			746			488			488		488			Lymnaea		Mollusk		Author reported half the detection limit
	Fatmucket Lampsilis siliquoidea		1055		604		040		Biomass			169			104			104		104			Lampsilis		Mollusk		Author reported
	Cladoceran Ceriodaphnia dubia		50		715		11		Reproduction - youngstarting adult			1780			190						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		26		73		05		Reproduction - youngfemale			1557			272						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		46		75		05		Reproduction - youngfemale			8087			110						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		96		79		05		Reproduction - youngfemale			6519			80						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		194		81		05		Reproduction - youngfemale			6836			89						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		255		703		05		Reproduction (youngfemale)			250			56						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		122		713		05		Reproduction (youngfemale)			860			106						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		25		798		05		Reproduction (youngfemale)			700			101						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		61		803		05		Reproduction (youngfemale)			1010			136						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		122		810		05		Reproduction (youngfemale)			870			116						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		255		633		05		Reproduction (youngfemale)			260			176						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		121		636		05		Reproduction (youngfemale)			390			110			118		118			Ceriodaphnia		Invert		Author reported
	Amphipod Hyalella azteca		95		61		051		Biomass (28 d)			1993			101						Hyalella			Author reported
	Amphipod Hyalella azteca		106		604		033		Biomass (28 d)			425			289			171		171			Hyalella		Invert		Author reported
	Midge Chironomus riparius		118		558		18		Adult midge emergence			2955			119						Chironomus			Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Midge Chironomus riparius		119		505		18		Adult midge emergence			8442			2258						Chironomus			Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Midge Chironomus riparius		91		66		051		Reproduction ( of eggscase)			3387			766			591		591			Chironomus		Invert		Author reported
	Atlantic salmon Salmo salar		127		57		18		Biomass			6156			1112			1112		1112			Salmo		Fish		Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Brook trout Salvelinus fontinalis		123		655		19		Biomass			1644			59						Salvelinus			Author reported		wellRO mix
	Brook trout Salvelinus fontinalis		128		565		18		Biomass			1435			283			1293		1293			Salvelinus		Fish		Author reported		wellRO mix
	Fathead minnow Pimephales promelas		220		770		16		Biomass			6194			781						Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		well water
	Fathead minnow Pimephales promelas		96		620		025		Fry survival			4286			379			544		544			Pimephales		Fish		Author reported half the detection limit
	Zebrafish Danio rerio		83		61		025		Biomass			2344			241			241		241			Danio		Fish		Author reported half the detection limit
	Wood frog Rana sylvatica		115		469		16		MATC-survival		gt		2000		gt		2131		gt		2131		2131		gt		Rana		Amphib - Other Data		Value is from 2007 FW Cu AWQC Appendix C recommendation		aged tap water

	Appendix A Acceptable Acute Toxicity Data of Aluminum to Freshwater Aquatic Animals
	Species		Methoda		Hardness (mgL as CaCO3)		pH		DOC(mgL)			LC50 or EC50 Conc (microgL)		Reference			Normalized Conc - LC50(microgL)			Species Mean Acute Value(microgL)		Genus Mean Acute Value(microgL)			Genus			DOC Note		Dilution water
	Snail Physa sp		S M T		474		659		11		gt		23400		Call 1984 Call et al 1984		gt		4843			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		755		11			30600		Call 1984 Call et al 1984			2699			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		817		11		gt		24700		Call 1984 Call et al 1984		gt		2404			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		746		11			55500		Call 1984 Call et al 1984			5034			3546		3546			Physa		Mollusk		Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Fatmucket Lampsilis siliquoidea		F M T		1060		612		048		gt		6302		Wang et al 2016 Wang et al 2017		gt		2983		gt		2983		2983		gt		Lampsilis		Mollusk		Author reported
	Cladoceran Ceriodaphnia dubia		S M A		500		742		11			1900		McCauley et al 1986			173			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		S M A		505		786		11			1500		McCauley et al 1986			129			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		UW-S Lake Superior lab water
	Cladoceran Ceriodaphnia dubia		S M A		500		813		11			2560		McCauley et al 1986			243			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		R M T		26		75		05			720		ENSR 1992d			111			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		R M T		46		76		05			1880		ENSR 1992d			250			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		R M T		96		78		05			2450		ENSR 1992d			289			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		S U NR		90		715		05			3727		Fort and Stover 1995			500			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U NR		90		715		05			5673		Fort and Stover 1995			761			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U NR		89		76		05			2880		Soucek et al 2001			336			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		601		05			7112		European Al Association 2009			169			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		605		2			6865		European Al Association 2009			709			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		609		4			15581		European Al Association 2009			1007			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		601		05			681		European Al Association 2009			162			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		603		05			1630		European Al Association 2009			367			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		597		05			1785		European Al Association 2009			473			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		592		05			1410		European Al Association 2009			429			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		699		05		gt		1300		European Al Association 2009		gt		419			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		785		05		gt		5000		European Al Association 2009		gt		796			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		68		2		gt		10000		European Al Association 2009		gt		2076			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		782		2		gt		15000		European Al Association 2009		gt		1163			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		677		4		gt		10000		European Al Association 2009		gt		1516			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		766		4		gt		15000		European Al Association 2009		gt		861			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		79		05		gt		2000		European Al Association 2009		gt		315			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		789		05		gt		2000		European Al Association 2009		gt		316			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		604		05			1108		European Al Association 2009			85			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		598		2			11371		European Al Association 2009			482			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		573		4			80467		European Al Association 2009			4357			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		671		05		gt		10000		European Al Association 2009		gt		2379			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		783		05		gt		5000		European Al Association 2009		gt		634			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		679		2		gt		10000		European Al Association 2009		gt		1039			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		767		2		gt		15000		European Al Association 2009		gt		908			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		668		4		gt		15000		European Al Association 2009		gt		1246			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		762		4		gt		15000		European Al Association 2009		gt		633			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		606		2			33868		European Al Association 2009			792			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		56		4			104842		European Al Association 2009			4880			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		693		05		gt		5000		European Al Association 2009		gt		713			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		120		788		05		gt		5000		European Al Association 2009		gt		590			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		120		676		2		gt		15000		European Al Association 2009		gt		1212			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		771		2		gt		15000		European Al Association 2009		gt		810			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		66		4		gt		15000		European Al Association 2009		gt		1014			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		76		4		gt		15000		European Al Association 2009		gt		555			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			11971		European Al Association 2010			270			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			27478		European Al Association 2010			619			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			11998		European Al Association 2010			270			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		607		05			92495		European Al Association 2010			188			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		609		05			31337		European Al Association 2010			604			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		61		05			33235		European Al Association 2010			624			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		708		05		gt		8864		European Al Association 2010		gt		253			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		779		05		gt		42783		European Al Association 2010		gt		693			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		753		05			13204		European Al Association 2010			24			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		600		601		05			46326		European Al Association 2010			380			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		600		599		05		gt		8590		European Al Association 2010		gt		737			509				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia reticulata		F M T		451		60		11			304		Shephard 1983			201			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia reticulata		F M T		40		55		11			362		Shephard 1983			5531			1054		733			Ceriodaphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake SuperiorDI Mix
	Cladoceran Daphnia magna		S U NR		453		774		11			3900		Biesinger and Christensen 1972			338			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Daphnia magna		S M T		220		76		16			38200		Kimball 1978			2030			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Well water
	Cladoceran Daphnia magna		S U T		451		725		11			2800		Shephard 1983			287			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Daphnia magna		S U T		168		599		05			5000		European Al Association 2009		gt		227			-				Daphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Daphnia magna		S U T		168		792		05			7950		European Al Association 2009			92			333		333			Daphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Ostracod Stenocypris major		R M T		1563		651		16			310196		Shuhaimi-Othman et al 2011a			1022			1022		1022			Stenocypris		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (Malaysia)
	Amphipod Crangonyx pseudogracilis		R U T		50		675		16			9190		Martin and Holdich 1986			1217			1217		1217			Crangonyx		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (UK)
	Amphipod Hyalella azteca		F M T		105		613		048		gt		5997		Wang et al 2016 Wang et al 2017		gt		2800		gt		2800		2800		gt		Hyalella		Invert		Author reported
	Stonefly Acroneuria sp		S M T		474		746		11		gt		22600		Call et al 1984		gt		2050		gt		2050		2050		gt		Acroneuria		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Midge Chironomus plumosus		S U T		80		70		16			30000		Fargasova 2001			2536			2536		2536			Chironomus		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (Slovakia)
	Midge Paratanytarsus dissimilis		S M T		1743		728		28		gt		77700		Lamb and Bailey 1981 1983		gt		6232		gt		6232		6232		gt		Paratanytarsus		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Liberty Lake Washington
	Rainbow trout Oncorhynchus mykiss		F M T		274		758		05		gt		9840		Gundersen et al 1994		gt		1256			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		452		762		05		gt		8070		Gundersen et al 1994		gt		1140			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		895		758		05		gt		8160		Gundersen et al 1994		gt		1489			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		1304		758		05		gt		8200		Gundersen et al 1994		gt		1676			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		232		825		05			6170		Gundersen et al 1994			260			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		350		826		05			6170		Gundersen et al 1994			333			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		836		829		05			7670		Gundersen et al 1994			701			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		1158		830		05			6930		Gundersen et al 1994			777			801		801			Oncorhynchus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Atlantic salmon Salmo salar		S U T		68		55		05			584		Hamilton and Haines 1995			4602			-				Salmo			Value is from 2007 FW Cu AWQC Appendix C recommendation		Soft recon
	Atlantic salmon Salmo salar		S U T		68		65		05			599		Hamilton and Haines 1995			531			1564		1564			Salmo		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Soft recon
	Brook trout Salvelinus fontinalis		S U T		40		56		16			6530		Tandjung 1982			6947			-				Salvelinus			Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Brook trout Salvelinus fontinalis		S U T		18		56		16			3400		Tandjung 1982			6209			-				Salvelinus			Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Brook trout Salvelinus fontinalis		S U T		2		56		16			370		Tandjung 1982			2988			5051		5051			Salvelinus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Green sunfish Lepomis cyanellus		S M T		474		755		11		gt		50000		Call et al 1984		gt		5255		gt		5255		5255		gt		Lepomis		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Rio Grande silvery minnow Hybognathus amarus		R M T		140		81		05		gt		59100		Buhl 2002		gt		8620		gt		8620		8620		gt		Hybognathus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon water
	Fathead minnow Pimephales promelas		S M T		474		761		11		gt		48200		Call et al 1984		gt		4707			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Fathead minnow Pimephales promelas		S M T		474		805		11		gt		49800		Call et al 1984		gt		2837			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Fathead minnow Pimephales promelas		R M T		26		78		05			1160		ENSR 1992c			104			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		46		76		05			8180		ENSR 1992c			1191			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		96		81		05			20300		ENSR 1992c			2398			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		194		81		05			44800		ENSR 1992c			7839			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		140		81		05		gt		59100		Buhl 2002		gt		8620			2224		2224			Pimephales		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon water
	Smallmouth bass Micropterus dolomieui		S M T		1245		505		16			130		Kane and Rabeni 1987			859			859		859			Micropterus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Green tree frog Hyla cinerea		R M T		455		549		05		gt		4052		Jung and Jagoe 1995		gt		4377		gt		4377		4377		gt		Hyla		Amphibian		Value is from 2007 FW Cu AWQC Appendix C recommendation		artificial soft recon

	Normalization Chemistry
	pH		6					FAV		360
	Hardness		25					CMC		180
	DOC		1					CCC		99
	Acute Ranked GMAV											Chronic Ranked GMCV
	Rank			GMAV		Genus								Rank			GMCV		Genus
	18		gt		8620		Hybognathus		Fish							12		gt		2131		Rana		Amphib - Other Data
	17		gt		6232		Paratanytarsus		Invert							11			1710		Aeolosoma		Invert
	16		gt		5255		Lepomis		Fish							10			591		Chironomus		Invert
	15			5051		Salvelinus		Fish							9			544		Pimephales		Fish
	14		gt		4377		Hyla		Amphibian							8			488		Lymnaea		Mollusk
	13			3546		Physa		Mollusk							7			255		Brachionus		Invert
	12		gt		2983		Lampsilis		Mollusk							6			241		Danio		Fish
	11		gt		2800		Hyalella		Invert							5			171		Hyalella		Invert
	10			2536		Chironomus		Invert							4			129		Salvelinus		Fish
	9			2224		Pimephales		Fish							3			118		Ceriodaphnia		Invert
	8		gt		2050		Acroneuria		Invert							2			111		Salmo		Fish
	7			1564		Salmo		Fish							1			104		Lampsilis		Mollusk
	6			1217		Crangonyx		Invert
	5			1022		Stenocypris		Invert
	4			859		Micropterus		Fish
	3			801		Oncorhynchus		Fish
	2			733		Ceriodaphnia		Invert
	1			333		Daphnia		Invert
		N		Rank		Genus		GMAV		ln(GMAV)		ln(GMAV)2		P=R(N+1)		sqrt(P)				N		Rank		Genus		GMCV		ln(GMCV)		ln(GMCV)2		P=R(N+1)		sqrt(P)
		18		4		Micropterus		859		676		4564		0211		0459				12		4		Salvelinus		129		486		2364		0308		0555
			3		Oncorhynchus		801		669		4469		0158		0397					3		Ceriodaphnia		118		477		2277		0231		0480
			2		Ceriodaphnia		733		660		4352		0105		0324					2		Salmo		111		471		2220		0154		0392
			1		Daphnia		333		581		3374		0053		0229					1		Lampsilis		104		465		2159		0077		0277
			Sum				2585		1676		0526		141					Sum				1899		902		0769		170
			S2 =		1987										S2 =		059
			L =		4890										L =		4420
			A =		5887										A =		4592
			FAV =		360										FCV =		987
			CMC =		180

				All concentrations reported are microgL total Aluminum
				lt-----Click Button after entering data
	ENTER DATA HERE									ERRORNUM		ACUTE														CHRONIC
										Rank 1				Rank 2				Rank 3				Rank 4					Rank 1				Rank 2				Rank 3				Rank 4
	SiteName		DOC(mgL)		Hardness(mgL as CaCO3)		pH			FAV		CMC		CCC			Genus		GMAV			Genus		GMAV			Genus		GMAV			Genus		GMAV				Genus		GMCV			Genus		GMCV			Genus		GMCV			Genus		GMCV
	Site 1		50		25		9		75428737034162		35229324666008		1800		770			Daphnia			33452		Ceriodaphnia			73576		Stenocypris			102576		Crangonyx			122230			Lampsilis			10463		Ceriodaphnia			11865		Hyalella			17200		Brachionus			25648
	Site 2		50		50		9		75428737034162		31204991509063		1600		680			Daphnia			29631		Ceriodaphnia			65171		Stenocypris			90859		Crangonyx			108267			Lampsilis			9268		Ceriodaphnia			10510		Hyalella			15236		Brachionus			22718
	Site 3		50		75		9		75428737034162		29067527928199		1500		640			Daphnia			27601		Ceriodaphnia			60707		Stenocypris			84635		Crangonyx			100851			Lampsilis			8633		Ceriodaphnia			9790		Hyalella			14192		Brachionus			21162
	Site 4		50		100		9		75428737034162		27640367912592		1400		600			Daphnia			26246		Ceriodaphnia			57726		Stenocypris			80480		Crangonyx			95899			Lampsilis			8209		Ceriodaphnia			9309		Hyalella			13495		Brachionus			20123
	Site 5		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 6		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 7		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 8		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 9		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 10		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 11		50		25		85		75428737034162		63245275664384		3200		1400			Daphnia			60055		Ceriodaphnia			132086		Stenocypris			184149		Crangonyx			219432			Lampsilis			18783		Ceriodaphnia			21301		Hyalella			30879		Brachionus			46045
	Site 12		50		50		85		75428737034162		61388044609926		3100		1300			Daphnia			58291		Ceriodaphnia			128208		Stenocypris			178742		Crangonyx			212988			Lampsilis			18232		Ceriodaphnia			20676		Hyalella			29972		Brachionus			44693
	Site 13		50		75		85		75428737034162		60327020429132		3000		1300			Daphnia			57284		Ceriodaphnia			125992		Stenocypris			175652		Crangonyx			209307			Lampsilis			17917		Ceriodaphnia			20318		Hyalella			29454		Brachionus			43920
	Site 14		50		100		85		75428737034162		59585352130146		3000		1300			Daphnia			56580		Ceriodaphnia			124443		Stenocypris			173493		Crangonyx			206734			Lampsilis			17696		Ceriodaphnia			20068		Hyalella			29092		Salmo			41264
	Site 15		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 16		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 17		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 18		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 19		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 20		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370

	Appendix C Acceptable Chronic Toxicity Data of Aluminum to Freshwater Aquatic Animals
									MLR equation
	Species		Hardness (mgL as CaCO3)		pH		DOC(mgL)		EC20 Endpoint			EC20(microgL)			Normalized Conc- EC20(microgL)			SMCV-EC20(microgL)		GMCV-EC20(microgL)			Genus			DOC Notes		Dilution water
	Oligochaete Aeolosoma sp		48		6		025		Reproduction (population size)			1235			17098			17098		17098			Aeolosoma		Invert		Author reported half the detection limit
	Rotifer Brachionus calyciflorus		100		62		025		Reproduction (population size)			431			2555			2555		2555			Brachionus		Invert		Author reported half the detection limit
	Great pond snail Lymnaea stagnalis		117		61		025		Biomass			746			4877			4877		4877			Lymnaea		Mollusk		Author reported half the detection limit
	Fatmucket Lampsilis siliquoidea		1055		604		040		Biomass			169			1042			1042		1042			Lampsilis		Mollusk		Author reported
	Cladoceran Ceriodaphnia dubia		50		715		11		Reproduction - youngstarting adult			1780			1898						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		26		73		05		Reproduction - youngfemale			1557			2718						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		46		75		05		Reproduction - youngfemale			8087			1102						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		96		79		05		Reproduction - youngfemale			6519			796						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		194		81		05		Reproduction - youngfemale			6836			888						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		255		703		05		Reproduction (youngfemale)			250			563						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		122		713		05		Reproduction (youngfemale)			860			1061						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		25		798		05		Reproduction (youngfemale)			700			1006						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		61		803		05		Reproduction (youngfemale)			1010			1363						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		122		810		05		Reproduction (youngfemale)			870			1164						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		255		633		05		Reproduction (youngfemale)			260			1758						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		121		636		05		Reproduction (youngfemale)			390			1101			1182		1182			Ceriodaphnia		Invert		Author reported
	Amphipod Hyalella azteca		95		61		051		Biomass (28 d)			1993			1014						Hyalella			Author reported
	Amphipod Hyalella azteca		106		604		033		Biomass (28 d)			425			2895			1713		1713			Hyalella		Invert		Author reported
	Midge Chironomus riparius		118		558		18		Adult midge emergence			2955			1192						Chironomus			Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Midge Chironomus riparius		119		505		18		Adult midge emergence			8442			22578						Chironomus			Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Midge Chironomus riparius		91		66		051		Reproduction ( of eggscase)			3387			7664			5908		5908			Chironomus		Invert		Author reported
	Atlantic salmon Salmo salar		127		57		18		Biomass			6156			5085			5085		5085			Salmo		Fish		Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Brook trout Salvelinus fontinalis		123		655		19		Biomass			1644			270						Salvelinus			Author reported		wellRO mix
	Brook trout Salvelinus fontinalis		128		565		18		Biomass			1435			1295			5914		5914			Salvelinus		Fish		Author reported		wellRO mix
	Fathead minnow Pimephales promelas		220		770		16		Biomass			6194			3569						Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		well water
	Fathead minnow Pimephales promelas		96		620		025		Fry survival			4286			1734			2488		2488			Pimephales		Fish		Author reported half the detection limit
	Zebrafish Danio rerio		83		61		025		Biomass			2344			1102			1102		1102			Danio		Fish		Author reported half the detection limit
	Wood frog Rana sylvatica		115		469		16		MATC-survival		gt		2000		gt		9746		gt		9746		9746		gt		Rana		Amphib - Other Data		Value is from 2007 FW Cu AWQC Appendix C recommendation		aged tap water

	Appendix A Acceptable Acute Toxicity Data of Aluminum to Freshwater Aquatic Animals
	Species		Methoda		Hardness (mgL as CaCO3)		pH		DOC(mgL)			LC50 or EC50 Conc (microgL)		Reference			Normalized Conc - LC50(microgL)			Species Mean Acute Value(microgL)		Genus Mean Acute Value(microgL)			Genus			DOC Note		Dilution water
	Snail Physa sp		S M T		474		659		11		gt		23400		Call 1984 Call et al 1984		gt		48425			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		755		11			30600		Call 1984 Call et al 1984			26986			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		817		11		gt		24700		Call 1984 Call et al 1984		gt		24040			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		746		11			55500		Call 1984 Call et al 1984			50338			35462		35462			Physa		Mollusk		Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Fatmucket Lampsilis siliquoidea		F M T		1060		612		048		gt		6302		Wang et al 2016 Wang et al 2017		gt		29834		gt		29834		29834		gt		Lampsilis		Mollusk		Author reported
	Cladoceran Ceriodaphnia dubia		S M A		500		742		11			1900		McCauley et al 1986			1728			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		S M A		505		786		11			1500		McCauley et al 1986			1294			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		UW-S Lake Superior lab water
	Cladoceran Ceriodaphnia dubia		S M A		500		813		11			2560		McCauley et al 1986			2431			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		R M T		26		75		05			720		ENSR 1992d			1113			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		R M T		46		76		05			1880		ENSR 1992d			2495			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		R M T		96		78		05			2450		ENSR 1992d			2894			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		S U NR		90		715		05			3727		Fort and Stover 1995			5002			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U NR		90		715		05			5673		Fort and Stover 1995			7613			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U NR		89		76		05			2880		Soucek et al 2001			3362			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		601		05			7112		European Al Association 2009			1691			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		605		2			6865		European Al Association 2009			7088			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		609		4			15581		European Al Association 2009			10072			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		601		05			681		European Al Association 2009			1620			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		603		05			1630		European Al Association 2009			3674			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		597		05			1785		European Al Association 2009			4732			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		592		05			1410		European Al Association 2009			4295			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		699		05		gt		1300		European Al Association 2009		gt		4189			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		785		05		gt		5000		European Al Association 2009		gt		7960			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		68		2		gt		10000		European Al Association 2009		gt		20755			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		782		2		gt		15000		European Al Association 2009		gt		11626			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		677		4		gt		10000		European Al Association 2009		gt		15161			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		766		4		gt		15000		European Al Association 2009		gt		8608			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		79		05		gt		2000		European Al Association 2009		gt		3150			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		789		05		gt		2000		European Al Association 2009		gt		3156			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		604		05			1108		European Al Association 2009			850			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		598		2			11371		European Al Association 2009			4817			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		573		4			80467		European Al Association 2009			43570			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		671		05		gt		10000		European Al Association 2009		gt		23786			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		783		05		gt		5000		European Al Association 2009		gt		6344			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		679		2		gt		10000		European Al Association 2009		gt		10394			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		767		2		gt		15000		European Al Association 2009		gt		9082			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		668		4		gt		15000		European Al Association 2009		gt		12461			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		762		4		gt		15000		European Al Association 2009		gt		6332			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		606		2			33868		European Al Association 2009			7920			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		56		4			104842		European Al Association 2009			48800			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		693		05		gt		5000		European Al Association 2009		gt		7126			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		120		788		05		gt		5000		European Al Association 2009		gt		5898			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		120		676		2		gt		15000		European Al Association 2009		gt		12117			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		771		2		gt		15000		European Al Association 2009		gt		8096			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		66		4		gt		15000		European Al Association 2009		gt		10139			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		76		4		gt		15000		European Al Association 2009		gt		5545			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			11971		European Al Association 2010			2699			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			27478		European Al Association 2010			6194			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			11998		European Al Association 2010			2705			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		607		05			92495		European Al Association 2010			1876			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		609		05			31337		European Al Association 2010			6035			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		61		05			33235		European Al Association 2010			6238			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		708		05		gt		8864		European Al Association 2010		gt		2534			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		779		05		gt		42783		European Al Association 2010		gt		6930			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		753		05			13204		European Al Association 2010			244			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		600		601		05			46326		European Al Association 2010			3798			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		600		599		05		gt		8590		European Al Association 2010		gt		7366			5094				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia reticulata		F M T		451		60		11			304		Shephard 1983			2009			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia reticulata		F M T		40		55		11			362		Shephard 1983			55307			10542		7328			Ceriodaphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake SuperiorDI Mix
	Cladoceran Daphnia magna		S U NR		453		774		11			3900		Biesinger and Christensen 1972			3382			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Daphnia magna		S M T		220		76		16			38200		Kimball 1978			20303			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Well water
	Cladoceran Daphnia magna		S U T		451		725		11			2800		Shephard 1983			2868			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Daphnia magna		S U T		168		599		05			5000		European Al Association 2009		gt		2265			-				Daphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Daphnia magna		S U T		168		792		05			7950		European Al Association 2009			920			3332		3332			Daphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Ostracod Stenocypris major		R M T		1563		651		16			310196		Shuhaimi-Othman et al 2011a			10216			10216		10216			Stenocypris		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (Malaysia)
	Amphipod Crangonyx pseudogracilis		R U T		50		675		16			9190		Martin and Holdich 1986			12174			12174		12174			Crangonyx		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (UK)
	Amphipod Hyalella azteca		F M T		105		613		048		gt		5997		Wang et al 2016 Wang et al 2017		gt		28002		gt		28002		28002		gt		Hyalella		Invert		Author reported
	Stonefly Acroneuria sp		S M T		474		746		11		gt		22600		Call et al 1984		gt		20498		gt		20498		20498		gt		Acroneuria		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Midge Chironomus plumosus		S U T		80		70		16			30000		Fargasova 2001			25361			25361		25361			Chironomus		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (Slovakia)
	Midge Paratanytarsus dissimilis		S M T		1743		728		28		gt		77700		Lamb and Bailey 1981 1983		gt		62318		gt		62318		62318		gt		Paratanytarsus		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Liberty Lake Washington
	Rainbow trout Oncorhynchus mykiss		F M T		274		758		05		gt		9840		Gundersen et al 1994		gt		5745			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		452		762		05		gt		8070		Gundersen et al 1994		gt		5212			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		895		758		05		gt		8160		Gundersen et al 1994		gt		6807			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		1304		758		05		gt		8200		Gundersen et al 1994		gt		7662			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		232		825		05			6170		Gundersen et al 1994			1190			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		350		826		05			6170		Gundersen et al 1994			1523			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		836		829		05			7670		Gundersen et al 1994			3205			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		1158		830		05			6930		Gundersen et al 1994			3554			3661		3661			Oncorhynchus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Atlantic salmon Salmo salar		S U T		68		55		05			584		Hamilton and Haines 1995			21042			-				Salmo			Value is from 2007 FW Cu AWQC Appendix C recommendation		Soft recon
	Atlantic salmon Salmo salar		S U T		68		65		05			599		Hamilton and Haines 1995			2430			7151		7151			Salmo		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Soft recon
	Brook trout Salvelinus fontinalis		S U T		40		56		16			6530		Tandjung 1982			31767			-				Salvelinus			Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Brook trout Salvelinus fontinalis		S U T		18		56		16			3400		Tandjung 1982			28391			-				Salvelinus			Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Brook trout Salvelinus fontinalis		S U T		2		56		16			370		Tandjung 1982			13663			23097		23097			Salvelinus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Green sunfish Lepomis cyanellus		S M T		474		755		11		gt		50000		Call et al 1984		gt		24028		gt		24028		24028		gt		Lepomis		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Rio Grande silvery minnow Hybognathus amarus		R M T		140		81		05		gt		59100		Buhl 2002		gt		39414		gt		39414		39414		gt		Hybognathus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon water
	Fathead minnow Pimephales promelas		S M T		474		761		11		gt		48200		Call et al 1984		gt		21522			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Fathead minnow Pimephales promelas		S M T		474		805		11		gt		49800		Call et al 1984		gt		12972			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Fathead minnow Pimephales promelas		R M T		26		78		05			1160		ENSR 1992c			477			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		46		76		05			8180		ENSR 1992c			5446			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		96		81		05			20300		ENSR 1992c			10966			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		194		81		05			44800		ENSR 1992c			35847			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		140		81		05		gt		59100		Buhl 2002		gt		39414			10168		10168			Pimephales		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon water
	Smallmouth bass Micropterus dolomieui		S M T		1245		505		16			130		Kane and Rabeni 1987			3929			3929		3929			Micropterus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Green tree frog Hyla cinerea		R M T		455		549		05		gt		4052		Jung and Jagoe 1995		gt		20016		gt		20016		20016		gt		Hyla		Amphibian		Value is from 2007 FW Cu AWQC Appendix C recommendation		artificial soft recon

	Normalization Chemistry
	pH		7					FAV		2741
	Hardness		100					CMC		1400
	DOC		1					CCC		390
	Acute Ranked GMAV											Chronic Ranked GMCV
	Rank			GMAV		Genus								Rank			GMCV		Genus
	18		gt		62318		Paratanytarsus		Invert							12			17098		Aeolosoma		Invert
	17		gt		39414		Hybognathus		Fish							11		gt		9746		Rana		Amphib - Other Data
	16			35462		Physa		Mollusk							10			5908		Chironomus		Invert
	15		gt		29834		Lampsilis		Mollusk							9			4877		Lymnaea		Mollusk
	14		gt		28002		Hyalella		Invert							8			2555		Brachionus		Invert
	13			25361		Chironomus		Invert							7			2488		Pimephales		Fish
	12		gt		24028		Lepomis		Fish							6			1713		Hyalella		Invert
	11			23097		Salvelinus		Fish							5			1182		Ceriodaphnia		Invert
	10		gt		20498		Acroneuria		Invert							4			1102		Danio		Fish
	9		gt		20016		Hyla		Amphibian							3			1042		Lampsilis		Mollusk
	8			12174		Crangonyx		Invert							2			591		Salvelinus		Fish
	7			10216		Stenocypris		Invert							1			509		Salmo		Fish
	6			10168		Pimephales		Fish
	5			7328		Ceriodaphnia		Invert
	4			7151		Salmo		Fish
	3			3929		Micropterus		Fish
	2			3661		Oncorhynchus		Fish
	1			3332		Daphnia		Invert
		N		Rank		Genus		GMAV		ln(GMAV)		ln(GMAV)2		P=R(N+1)		sqrt(P)				N		Rank		Genus		GMCV		ln(GMCV)		ln(GMCV)2		P=R(N+1)		sqrt(P)
		18		4		Salmo		7151		887		7876		0211		0459				12		4		Danio		1102		700		4907		0308		0555
			3		Micropterus		3929		828		6849		0158		0397					3		Lampsilis		1042		695		4829		0231		0480
			2		Oncorhynchus		3661		821		6733		0105		0324					2		Salvelinus		591		638		4074		0154		0392
			1		Daphnia		3332		811		6579		0053		0229					1		Salmo		509		623		3883		0077		0277
			Sum				3347		2804		0526		141					Sum				2657		1769		0769		170
			S2 =		1223										S2 =		1080
			L =		7134										L =		5242
			A =		7916										A =		5976
			FAV =		2741										FCV =		3940
			CMC =		1371

				All concentrations reported are microgL total Aluminum
				lt-----Click Button after entering data
	ENTER DATA HERE									ERRORNUM		ACUTE														CHRONIC
										Rank 1				Rank 2				Rank 3				Rank 4					Rank 1				Rank 2				Rank 3				Rank 4
	SiteName		DOC(mgL)		Hardness(mgL as CaCO3)		pH			FAV		CMC		CCC			Genus		GMAV			Genus		GMAV			Genus		GMAV			Genus		GMAV				Genus		GMCV			Genus		GMCV			Genus		GMCV			Genus		GMCV
	Site 1		50		25		9		75428737034162		35229324666008		1800		770			Daphnia			33452		Ceriodaphnia			73576		Stenocypris			102576		Crangonyx			122230			Lampsilis			10463		Ceriodaphnia			11865		Hyalella			17200		Brachionus			25648
	Site 2		50		50		9		75428737034162		31204991509063		1600		680			Daphnia			29631		Ceriodaphnia			65171		Stenocypris			90859		Crangonyx			108267			Lampsilis			9268		Ceriodaphnia			10510		Hyalella			15236		Brachionus			22718
	Site 3		50		75		9		75428737034162		29067527928199		1500		640			Daphnia			27601		Ceriodaphnia			60707		Stenocypris			84635		Crangonyx			100851			Lampsilis			8633		Ceriodaphnia			9790		Hyalella			14192		Brachionus			21162
	Site 4		50		100		9		75428737034162		27640367912592		1400		600			Daphnia			26246		Ceriodaphnia			57726		Stenocypris			80480		Crangonyx			95899			Lampsilis			8209		Ceriodaphnia			9309		Hyalella			13495		Brachionus			20123
	Site 5		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 6		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 7		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 8		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 9		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 10		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 11		50		25		85		75428737034162		63245275664384		3200		1400			Daphnia			60055		Ceriodaphnia			132086		Stenocypris			184149		Crangonyx			219432			Lampsilis			18783		Ceriodaphnia			21301		Hyalella			30879		Brachionus			46045
	Site 12		50		50		85		75428737034162		61388044609926		3100		1300			Daphnia			58291		Ceriodaphnia			128208		Stenocypris			178742		Crangonyx			212988			Lampsilis			18232		Ceriodaphnia			20676		Hyalella			29972		Brachionus			44693
	Site 13		50		75		85		75428737034162		60327020429132		3000		1300			Daphnia			57284		Ceriodaphnia			125992		Stenocypris			175652		Crangonyx			209307			Lampsilis			17917		Ceriodaphnia			20318		Hyalella			29454		Brachionus			43920
	Site 14		50		100		85		75428737034162		59585352130146		3000		1300			Daphnia			56580		Ceriodaphnia			124443		Stenocypris			173493		Crangonyx			206734			Lampsilis			17696		Ceriodaphnia			20068		Hyalella			29092		Salmo			41264
	Site 15		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 16		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 17		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 18		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 19		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 20		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370

Multiple Linear Regression (MLR) Models to Account for Effects of Water Chemistry

bull MLR models were developed for each species (DeForest et al 2017)ndash 119862119862 119889119889119906119906119887119887119894119894119886119886 11986411986411986211986220=119890119890[minus41026+[0525timesln(119863119863119874119874119862119862)]+[2201timesln(ℎ119886119886119903119903119889119889)]+(11282times119901119901119867119867)minus(0633times1199011199011198671198672)minus[0264times119901119901119867119867ln(ℎ119886119886119903119903119889119889)]]

ndash 119875119875 119901119901119903119903119900119900119898119898119890119890119897119897119886119886119904119904 11986411986411986211986220=119890119890[minus14029+[0503timesln(119863119863119874119874119862119862)]+[3443timesln(ℎ119886119886119903119903119889119889)]+(3131times119901119901119867119867)minus[0494times119901119901119867119867ln(ℎ119886119886119903119903119889119889)]]

bull These models were applied to both the acute and chronic aluminum toxicity datandash The C dubia model was applied to invertebrate datandash The P promelas model was applied to vertebrate data

19

III Normalize toxicity data in species sensitivity distribution to a set of water

quality conditionsbull Just like single parameter hardness models the MLR

models were used to normalize the aluminum toxicity data to a single set of water quality conditionsndash As an illustration for one set of calculations the draft

aluminum AWQC set these conditions to pH 7 hardness of 100 mgL and DOC of 1 mgL

bull This allows for comparisons to be made between species based on their sensitivity to aluminumndash Apples to apples

20

IV Construct sensitivity distribution to determine criteria at set conditions

21

Figure 8 Ranked Summary of Total Aluminum Genus Mean Acute Values (GMAVs) - Freshwater at pH 7 Hardness of 100 mgL and DOC of 1 mgL

IV Construct sensitivity distribution to determine criteria at set conditions

22

Figure 10 Ranked Summary of Total Aluminum Genus Mean Chronic Values (GMCVs) ndash Freshwater Supplemented with Other Data to Fulfill Missing MDRs at pH 7 Hardness of 100 mgL and DOC of 1 mgL

Aluminum Criteria Calculatorbull The relative ranking of genera in the sensitivity

distribution to which the toxicity data are normalized will change based on the set of water chemistry conditions (ie pH hardness and DOC)

bull To make calculating aluminum criteria easier EPA developed an Aluminum Criteria Calculatorndash Automated so that user only needs to enter water chemistry

conditions of interestndash Allows quick calculations for a variety of conditionsndash Limits calculations to the recommended conditions for which

the MLR models were developed were specified for hardness and DOC

bull No extrapolation beyond hardness and DOC empirical data bounds

23

Aluminum Criteria Calculator SD Example

24

pH=7 Hardness=100 DOC=1 pH=6 Hardness=25 DOC=1

Chronic Ranked GMCVRank GMCV Genus

12 17098 Aeolosoma Invert11 gt 9746 Rana Amphib - Other Data10 5908 Chironomus Invert

9 4877 Lymnaea Mollusk8 2555 Brachionus Invert7 2488 Pimephales Fish6 1713 Hyalella Invert5 1182 Ceriodaphnia Invert4 1102 Danio Fish3 1042 Lampsilis Mollusk2 591 Salvelinus Fish1 509 Salmo Fish

FCV = 3940 microgL total aluminum

Chronic Ranked GMCVRank GMCV Genus

12 gt 2131 Rana Amphib - Other Data11 1710 Aeolosoma Invert10 591 Chironomus Invert

9 544 Pimephales Fish8 488 Lymnaea Mollusk7 255 Brachionus Invert6 241 Danio Fish5 171 Hyalella Invert4 129 Salvelinus Fish3 118 Ceriodaphnia Invert2 111 Salmo Fish1 104 Lampsilis Mollusk

FCV = 987 microgL total aluminum

Multiple Scenarios

Run Scenarios

Summary Sheet

Acute Dataset

Chronic Dataset

Multiple Scenarios

Run Scenarios

Summary Sheet

Acute Dataset

Chronic Dataset

Boundaries of the Criteria Outputsbull MLR models were developed using data that

encompassndash DOC range of 008-5 mgLndash Hardness range of 98-127 mgL (as CaCO3)ndash pH range of 6-81

bull MLR criteria outputs are bounded atndash Maximum of 150 mgL total hardness (as CaCO3)

ndash DOC of 50 mgLndash pH range of the model is 50 to 90

25

Aluminum Criteria Look-Up Tables

26

bull EPA also provided criteria look-up tables in Appendix K

Har

dnes

s

CCC(microgL total aluminum)

(DOC=05 mgL)

pH 50 pH 55 pH 60 pH 65 pH 70 pH 75 pH 80 pH 85 pH 90

25 23 a 14 b 69 c 160 f 350 f 620 i 540 b 410 a 230 a

50 43 a 24 b 100 d 170 f 310 f 630 f 670 j 400 a 200 a

75 61 a 33 b 130 e 180 f 290 f 530 f 730 i 390 a 190 a

100 78 a 41 b 150 e 190 f 280 g 460 f 770 c 390 a 180 a

150 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

200 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

250 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

300 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

350 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

400 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

Aluminum Criteria Look-Up Tables

27

bull EPA also provided criteria look-up tables in Appendix K

Har

dnes

s

CCC(microgL total aluminum)

(DOC=10 mgL)

pH 50 pH 55 pH 60 pH 65 pH 70 pH 75 pH 80 pH 85 pH 90

25 33 a 21 b 99 c 220 f 490 f 900 i 780 b 590 a 330 a

50 61 a 35 b 150 e 250 f 440 f 890 f 960 j 580 a 290 a

75 88 a 47 b 180 e 260 f 410 f 740 f 1100 i 570 a 270 a

100 11 a 59 b 210 e 270 f 390 g 650 f 1100 c 560 b 260 a

150 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

200 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

250 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

300 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

350 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

400 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

Aluminum Criteria Look-Up Tables

28

bull EPA also provided criteria look-up tables in Appendix K

Har

dnes

s

CCC(microgL total aluminum)

(DOC=25 mgL)

pH 50 pH 55 pH 60 pH 65 pH 70 pH 75 pH 80 pH 85 pH 90

25 54 a 34 b 160 c 350 f 770 f 1500 i 1300 b 960 a 540 a

50 99 a 57 b 230 e 390 f 690 f 1400 f 1600 j 930 a 470 a

75 14 a 77 b 290 e 410 f 640 f 1200 f 1700 i 920 a 440 a

100 18 a 96 b 330 e 420 f 620 g 1000 f 1800 d 920 b 420 a

150 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

200 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

250 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

300 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

350 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

400 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

Aluminum Criteria Look-Up Tables

29

bull EPA also provided criteria look-up tables in Appendix K

Har

dnes

s

CCC(microgL total aluminum)

(DOC=50 mgL)

pH 50 pH 55 pH 60 pH 65 pH 70 pH 75 pH 80 pH 85 pH 90

25 77 a 49 b 230 c 500 f 1100 f 2100 c 1900 b 1400 a 770 a

50 14 a 82 b 330 e 540 f 970 f 2000 f 2200 j 1300 a 680 a

75 20 a 110 b 410 e 570 f 900 f 1600 f 2500 i 1300 a 640 a

100 26 a 140 b 460 f 590 f 880 g 1400 f 2500 d 1300 b 600 a

150 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

200 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

250 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

300 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

350 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

400 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

Status

30

bull Draft Document Released to the Federal Register on 72817ndash Public comment period of 60 days ndash Due Date for Comments is 92617

bull Revisions will be made considering public commentsbull Subsequent EPA intra-agency review of final criteria

document will then be conducted bull Publication of final criteria document planned for Late Fall

2017

Federal Register Webpagehttpswwwfederalregistergovdocuments201

707282017-15968request-for-scientific-views-draft-updated-aquatic-life-ambient-water-quality-criteria-for-aluminum

Aluminum Criteria Webpagehttpswwwepagovwqc2017-draft-aquatic-life-

criteria-aluminum-freshwater-documents

31

	Appendix C Acceptable Chronic Toxicity Data of Aluminum to Freshwater Aquatic Animals
									MLR equation
	Species		Hardness (mgL as CaCO3)		pH		DOC(mgL)		EC20 Endpoint			EC20(microgL)			Normalized Conc- EC20(microgL)			SMCV-EC20(microgL)		GMCV-EC20(microgL)			Genus			DOC Notes		Dilution water
	Oligochaete Aeolosoma sp		48		6		025		Reproduction (population size)			1235			1710			1710		1710			Aeolosoma		Invert		Author reported half the detection limit
	Rotifer Brachionus calyciflorus		100		62		025		Reproduction (population size)			431			255			255		255			Brachionus		Invert		Author reported half the detection limit
	Great pond snail Lymnaea stagnalis		117		61		025		Biomass			746			488			488		488			Lymnaea		Mollusk		Author reported half the detection limit
	Fatmucket Lampsilis siliquoidea		1055		604		040		Biomass			169			104			104		104			Lampsilis		Mollusk		Author reported
	Cladoceran Ceriodaphnia dubia		50		715		11		Reproduction - youngstarting adult			1780			190						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		26		73		05		Reproduction - youngfemale			1557			272						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		46		75		05		Reproduction - youngfemale			8087			110						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		96		79		05		Reproduction - youngfemale			6519			80						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		194		81		05		Reproduction - youngfemale			6836			89						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		255		703		05		Reproduction (youngfemale)			250			56						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		122		713		05		Reproduction (youngfemale)			860			106						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		25		798		05		Reproduction (youngfemale)			700			101						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		61		803		05		Reproduction (youngfemale)			1010			136						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		122		810		05		Reproduction (youngfemale)			870			116						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		255		633		05		Reproduction (youngfemale)			260			176						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		121		636		05		Reproduction (youngfemale)			390			110			118		118			Ceriodaphnia		Invert		Author reported
	Amphipod Hyalella azteca		95		61		051		Biomass (28 d)			1993			101						Hyalella			Author reported
	Amphipod Hyalella azteca		106		604		033		Biomass (28 d)			425			289			171		171			Hyalella		Invert		Author reported
	Midge Chironomus riparius		118		558		18		Adult midge emergence			2955			119						Chironomus			Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Midge Chironomus riparius		119		505		18		Adult midge emergence			8442			2258						Chironomus			Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Midge Chironomus riparius		91		66		051		Reproduction ( of eggscase)			3387			766			591		591			Chironomus		Invert		Author reported
	Atlantic salmon Salmo salar		127		57		18		Biomass			6156			1112			1112		1112			Salmo		Fish		Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Brook trout Salvelinus fontinalis		123		655		19		Biomass			1644			59						Salvelinus			Author reported		wellRO mix
	Brook trout Salvelinus fontinalis		128		565		18		Biomass			1435			283			1293		1293			Salvelinus		Fish		Author reported		wellRO mix
	Fathead minnow Pimephales promelas		220		770		16		Biomass			6194			781						Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		well water
	Fathead minnow Pimephales promelas		96		620		025		Fry survival			4286			379			544		544			Pimephales		Fish		Author reported half the detection limit
	Zebrafish Danio rerio		83		61		025		Biomass			2344			241			241		241			Danio		Fish		Author reported half the detection limit
	Wood frog Rana sylvatica		115		469		16		MATC-survival		gt		2000		gt		2131		gt		2131		2131		gt		Rana		Amphib - Other Data		Value is from 2007 FW Cu AWQC Appendix C recommendation		aged tap water

	Appendix A Acceptable Acute Toxicity Data of Aluminum to Freshwater Aquatic Animals
	Species		Methoda		Hardness (mgL as CaCO3)		pH		DOC(mgL)			LC50 or EC50 Conc (microgL)		Reference			Normalized Conc - LC50(microgL)			Species Mean Acute Value(microgL)		Genus Mean Acute Value(microgL)			Genus			DOC Note		Dilution water
	Snail Physa sp		S M T		474		659		11		gt		23400		Call 1984 Call et al 1984		gt		4843			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		755		11			30600		Call 1984 Call et al 1984			2699			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		817		11		gt		24700		Call 1984 Call et al 1984		gt		2404			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		746		11			55500		Call 1984 Call et al 1984			5034			3546		3546			Physa		Mollusk		Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Fatmucket Lampsilis siliquoidea		F M T		1060		612		048		gt		6302		Wang et al 2016 Wang et al 2017		gt		2983		gt		2983		2983		gt		Lampsilis		Mollusk		Author reported
	Cladoceran Ceriodaphnia dubia		S M A		500		742		11			1900		McCauley et al 1986			173			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		S M A		505		786		11			1500		McCauley et al 1986			129			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		UW-S Lake Superior lab water
	Cladoceran Ceriodaphnia dubia		S M A		500		813		11			2560		McCauley et al 1986			243			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		R M T		26		75		05			720		ENSR 1992d			111			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		R M T		46		76		05			1880		ENSR 1992d			250			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		R M T		96		78		05			2450		ENSR 1992d			289			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		S U NR		90		715		05			3727		Fort and Stover 1995			500			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U NR		90		715		05			5673		Fort and Stover 1995			761			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U NR		89		76		05			2880		Soucek et al 2001			336			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		601		05			7112		European Al Association 2009			169			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		605		2			6865		European Al Association 2009			709			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		609		4			15581		European Al Association 2009			1007			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		601		05			681		European Al Association 2009			162			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		603		05			1630		European Al Association 2009			367			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		597		05			1785		European Al Association 2009			473			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		592		05			1410		European Al Association 2009			429			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		699		05		gt		1300		European Al Association 2009		gt		419			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		785		05		gt		5000		European Al Association 2009		gt		796			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		68		2		gt		10000		European Al Association 2009		gt		2076			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		782		2		gt		15000		European Al Association 2009		gt		1163			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		677		4		gt		10000		European Al Association 2009		gt		1516			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		766		4		gt		15000		European Al Association 2009		gt		861			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		79		05		gt		2000		European Al Association 2009		gt		315			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		789		05		gt		2000		European Al Association 2009		gt		316			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		604		05			1108		European Al Association 2009			85			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		598		2			11371		European Al Association 2009			482			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		573		4			80467		European Al Association 2009			4357			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		671		05		gt		10000		European Al Association 2009		gt		2379			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		783		05		gt		5000		European Al Association 2009		gt		634			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		679		2		gt		10000		European Al Association 2009		gt		1039			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		767		2		gt		15000		European Al Association 2009		gt		908			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		668		4		gt		15000		European Al Association 2009		gt		1246			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		762		4		gt		15000		European Al Association 2009		gt		633			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		606		2			33868		European Al Association 2009			792			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		56		4			104842		European Al Association 2009			4880			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		693		05		gt		5000		European Al Association 2009		gt		713			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		120		788		05		gt		5000		European Al Association 2009		gt		590			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		120		676		2		gt		15000		European Al Association 2009		gt		1212			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		771		2		gt		15000		European Al Association 2009		gt		810			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		66		4		gt		15000		European Al Association 2009		gt		1014			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		76		4		gt		15000		European Al Association 2009		gt		555			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			11971		European Al Association 2010			270			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			27478		European Al Association 2010			619			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			11998		European Al Association 2010			270			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		607		05			92495		European Al Association 2010			188			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		609		05			31337		European Al Association 2010			604			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		61		05			33235		European Al Association 2010			624			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		708		05		gt		8864		European Al Association 2010		gt		253			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		779		05		gt		42783		European Al Association 2010		gt		693			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		753		05			13204		European Al Association 2010			24			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		600		601		05			46326		European Al Association 2010			380			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		600		599		05		gt		8590		European Al Association 2010		gt		737			509				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia reticulata		F M T		451		60		11			304		Shephard 1983			201			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia reticulata		F M T		40		55		11			362		Shephard 1983			5531			1054		733			Ceriodaphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake SuperiorDI Mix
	Cladoceran Daphnia magna		S U NR		453		774		11			3900		Biesinger and Christensen 1972			338			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Daphnia magna		S M T		220		76		16			38200		Kimball 1978			2030			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Well water
	Cladoceran Daphnia magna		S U T		451		725		11			2800		Shephard 1983			287			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Daphnia magna		S U T		168		599		05			5000		European Al Association 2009		gt		227			-				Daphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Daphnia magna		S U T		168		792		05			7950		European Al Association 2009			92			333		333			Daphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Ostracod Stenocypris major		R M T		1563		651		16			310196		Shuhaimi-Othman et al 2011a			1022			1022		1022			Stenocypris		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (Malaysia)
	Amphipod Crangonyx pseudogracilis		R U T		50		675		16			9190		Martin and Holdich 1986			1217			1217		1217			Crangonyx		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (UK)
	Amphipod Hyalella azteca		F M T		105		613		048		gt		5997		Wang et al 2016 Wang et al 2017		gt		2800		gt		2800		2800		gt		Hyalella		Invert		Author reported
	Stonefly Acroneuria sp		S M T		474		746		11		gt		22600		Call et al 1984		gt		2050		gt		2050		2050		gt		Acroneuria		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Midge Chironomus plumosus		S U T		80		70		16			30000		Fargasova 2001			2536			2536		2536			Chironomus		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (Slovakia)
	Midge Paratanytarsus dissimilis		S M T		1743		728		28		gt		77700		Lamb and Bailey 1981 1983		gt		6232		gt		6232		6232		gt		Paratanytarsus		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Liberty Lake Washington
	Rainbow trout Oncorhynchus mykiss		F M T		274		758		05		gt		9840		Gundersen et al 1994		gt		1256			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		452		762		05		gt		8070		Gundersen et al 1994		gt		1140			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		895		758		05		gt		8160		Gundersen et al 1994		gt		1489			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		1304		758		05		gt		8200		Gundersen et al 1994		gt		1676			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		232		825		05			6170		Gundersen et al 1994			260			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		350		826		05			6170		Gundersen et al 1994			333			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		836		829		05			7670		Gundersen et al 1994			701			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		1158		830		05			6930		Gundersen et al 1994			777			801		801			Oncorhynchus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Atlantic salmon Salmo salar		S U T		68		55		05			584		Hamilton and Haines 1995			4602			-				Salmo			Value is from 2007 FW Cu AWQC Appendix C recommendation		Soft recon
	Atlantic salmon Salmo salar		S U T		68		65		05			599		Hamilton and Haines 1995			531			1564		1564			Salmo		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Soft recon
	Brook trout Salvelinus fontinalis		S U T		40		56		16			6530		Tandjung 1982			6947			-				Salvelinus			Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Brook trout Salvelinus fontinalis		S U T		18		56		16			3400		Tandjung 1982			6209			-				Salvelinus			Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Brook trout Salvelinus fontinalis		S U T		2		56		16			370		Tandjung 1982			2988			5051		5051			Salvelinus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Green sunfish Lepomis cyanellus		S M T		474		755		11		gt		50000		Call et al 1984		gt		5255		gt		5255		5255		gt		Lepomis		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Rio Grande silvery minnow Hybognathus amarus		R M T		140		81		05		gt		59100		Buhl 2002		gt		8620		gt		8620		8620		gt		Hybognathus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon water
	Fathead minnow Pimephales promelas		S M T		474		761		11		gt		48200		Call et al 1984		gt		4707			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Fathead minnow Pimephales promelas		S M T		474		805		11		gt		49800		Call et al 1984		gt		2837			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Fathead minnow Pimephales promelas		R M T		26		78		05			1160		ENSR 1992c			104			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		46		76		05			8180		ENSR 1992c			1191			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		96		81		05			20300		ENSR 1992c			2398			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		194		81		05			44800		ENSR 1992c			7839			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		140		81		05		gt		59100		Buhl 2002		gt		8620			2224		2224			Pimephales		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon water
	Smallmouth bass Micropterus dolomieui		S M T		1245		505		16			130		Kane and Rabeni 1987			859			859		859			Micropterus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Green tree frog Hyla cinerea		R M T		455		549		05		gt		4052		Jung and Jagoe 1995		gt		4377		gt		4377		4377		gt		Hyla		Amphibian		Value is from 2007 FW Cu AWQC Appendix C recommendation		artificial soft recon

	Normalization Chemistry
	pH		6					FAV		360
	Hardness		25					CMC		180
	DOC		1					CCC		99
	Acute Ranked GMAV											Chronic Ranked GMCV
	Rank			GMAV		Genus								Rank			GMCV		Genus
	18		gt		8620		Hybognathus		Fish							12		gt		2131		Rana		Amphib - Other Data
	17		gt		6232		Paratanytarsus		Invert							11			1710		Aeolosoma		Invert
	16		gt		5255		Lepomis		Fish							10			591		Chironomus		Invert
	15			5051		Salvelinus		Fish							9			544		Pimephales		Fish
	14		gt		4377		Hyla		Amphibian							8			488		Lymnaea		Mollusk
	13			3546		Physa		Mollusk							7			255		Brachionus		Invert
	12		gt		2983		Lampsilis		Mollusk							6			241		Danio		Fish
	11		gt		2800		Hyalella		Invert							5			171		Hyalella		Invert
	10			2536		Chironomus		Invert							4			129		Salvelinus		Fish
	9			2224		Pimephales		Fish							3			118		Ceriodaphnia		Invert
	8		gt		2050		Acroneuria		Invert							2			111		Salmo		Fish
	7			1564		Salmo		Fish							1			104		Lampsilis		Mollusk
	6			1217		Crangonyx		Invert
	5			1022		Stenocypris		Invert
	4			859		Micropterus		Fish
	3			801		Oncorhynchus		Fish
	2			733		Ceriodaphnia		Invert
	1			333		Daphnia		Invert
		N		Rank		Genus		GMAV		ln(GMAV)		ln(GMAV)2		P=R(N+1)		sqrt(P)				N		Rank		Genus		GMCV		ln(GMCV)		ln(GMCV)2		P=R(N+1)		sqrt(P)
		18		4		Micropterus		859		676		4564		0211		0459				12		4		Salvelinus		129		486		2364		0308		0555
			3		Oncorhynchus		801		669		4469		0158		0397					3		Ceriodaphnia		118		477		2277		0231		0480
			2		Ceriodaphnia		733		660		4352		0105		0324					2		Salmo		111		471		2220		0154		0392
			1		Daphnia		333		581		3374		0053		0229					1		Lampsilis		104		465		2159		0077		0277
			Sum				2585		1676		0526		141					Sum				1899		902		0769		170
			S2 =		1987										S2 =		059
			L =		4890										L =		4420
			A =		5887										A =		4592
			FAV =		360										FCV =		987
			CMC =		180

				All concentrations reported are microgL total Aluminum
				lt-----Click Button after entering data
	ENTER DATA HERE									ERRORNUM		ACUTE														CHRONIC
										Rank 1				Rank 2				Rank 3				Rank 4					Rank 1				Rank 2				Rank 3				Rank 4
	SiteName		DOC(mgL)		Hardness(mgL as CaCO3)		pH			FAV		CMC		CCC			Genus		GMAV			Genus		GMAV			Genus		GMAV			Genus		GMAV				Genus		GMCV			Genus		GMCV			Genus		GMCV			Genus		GMCV
	Site 1		50		25		9		75428737034162		35229324666008		1800		770			Daphnia			33452		Ceriodaphnia			73576		Stenocypris			102576		Crangonyx			122230			Lampsilis			10463		Ceriodaphnia			11865		Hyalella			17200		Brachionus			25648
	Site 2		50		50		9		75428737034162		31204991509063		1600		680			Daphnia			29631		Ceriodaphnia			65171		Stenocypris			90859		Crangonyx			108267			Lampsilis			9268		Ceriodaphnia			10510		Hyalella			15236		Brachionus			22718
	Site 3		50		75		9		75428737034162		29067527928199		1500		640			Daphnia			27601		Ceriodaphnia			60707		Stenocypris			84635		Crangonyx			100851			Lampsilis			8633		Ceriodaphnia			9790		Hyalella			14192		Brachionus			21162
	Site 4		50		100		9		75428737034162		27640367912592		1400		600			Daphnia			26246		Ceriodaphnia			57726		Stenocypris			80480		Crangonyx			95899			Lampsilis			8209		Ceriodaphnia			9309		Hyalella			13495		Brachionus			20123
	Site 5		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 6		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 7		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 8		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 9		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 10		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 11		50		25		85		75428737034162		63245275664384		3200		1400			Daphnia			60055		Ceriodaphnia			132086		Stenocypris			184149		Crangonyx			219432			Lampsilis			18783		Ceriodaphnia			21301		Hyalella			30879		Brachionus			46045
	Site 12		50		50		85		75428737034162		61388044609926		3100		1300			Daphnia			58291		Ceriodaphnia			128208		Stenocypris			178742		Crangonyx			212988			Lampsilis			18232		Ceriodaphnia			20676		Hyalella			29972		Brachionus			44693
	Site 13		50		75		85		75428737034162		60327020429132		3000		1300			Daphnia			57284		Ceriodaphnia			125992		Stenocypris			175652		Crangonyx			209307			Lampsilis			17917		Ceriodaphnia			20318		Hyalella			29454		Brachionus			43920
	Site 14		50		100		85		75428737034162		59585352130146		3000		1300			Daphnia			56580		Ceriodaphnia			124443		Stenocypris			173493		Crangonyx			206734			Lampsilis			17696		Ceriodaphnia			20068		Hyalella			29092		Salmo			41264
	Site 15		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 16		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 17		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 18		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 19		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 20		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370

	Appendix C Acceptable Chronic Toxicity Data of Aluminum to Freshwater Aquatic Animals
									MLR equation
	Species		Hardness (mgL as CaCO3)		pH		DOC(mgL)		EC20 Endpoint			EC20(microgL)			Normalized Conc- EC20(microgL)			SMCV-EC20(microgL)		GMCV-EC20(microgL)			Genus			DOC Notes		Dilution water
	Oligochaete Aeolosoma sp		48		6		025		Reproduction (population size)			1235			17098			17098		17098			Aeolosoma		Invert		Author reported half the detection limit
	Rotifer Brachionus calyciflorus		100		62		025		Reproduction (population size)			431			2555			2555		2555			Brachionus		Invert		Author reported half the detection limit
	Great pond snail Lymnaea stagnalis		117		61		025		Biomass			746			4877			4877		4877			Lymnaea		Mollusk		Author reported half the detection limit
	Fatmucket Lampsilis siliquoidea		1055		604		040		Biomass			169			1042			1042		1042			Lampsilis		Mollusk		Author reported
	Cladoceran Ceriodaphnia dubia		50		715		11		Reproduction - youngstarting adult			1780			1898						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		26		73		05		Reproduction - youngfemale			1557			2718						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		46		75		05		Reproduction - youngfemale			8087			1102						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		96		79		05		Reproduction - youngfemale			6519			796						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		194		81		05		Reproduction - youngfemale			6836			888						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		255		703		05		Reproduction (youngfemale)			250			563						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		122		713		05		Reproduction (youngfemale)			860			1061						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		25		798		05		Reproduction (youngfemale)			700			1006						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		61		803		05		Reproduction (youngfemale)			1010			1363						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		122		810		05		Reproduction (youngfemale)			870			1164						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		255		633		05		Reproduction (youngfemale)			260			1758						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		121		636		05		Reproduction (youngfemale)			390			1101			1182		1182			Ceriodaphnia		Invert		Author reported
	Amphipod Hyalella azteca		95		61		051		Biomass (28 d)			1993			1014						Hyalella			Author reported
	Amphipod Hyalella azteca		106		604		033		Biomass (28 d)			425			2895			1713		1713			Hyalella		Invert		Author reported
	Midge Chironomus riparius		118		558		18		Adult midge emergence			2955			1192						Chironomus			Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Midge Chironomus riparius		119		505		18		Adult midge emergence			8442			22578						Chironomus			Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Midge Chironomus riparius		91		66		051		Reproduction ( of eggscase)			3387			7664			5908		5908			Chironomus		Invert		Author reported
	Atlantic salmon Salmo salar		127		57		18		Biomass			6156			5085			5085		5085			Salmo		Fish		Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Brook trout Salvelinus fontinalis		123		655		19		Biomass			1644			270						Salvelinus			Author reported		wellRO mix
	Brook trout Salvelinus fontinalis		128		565		18		Biomass			1435			1295			5914		5914			Salvelinus		Fish		Author reported		wellRO mix
	Fathead minnow Pimephales promelas		220		770		16		Biomass			6194			3569						Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		well water
	Fathead minnow Pimephales promelas		96		620		025		Fry survival			4286			1734			2488		2488			Pimephales		Fish		Author reported half the detection limit
	Zebrafish Danio rerio		83		61		025		Biomass			2344			1102			1102		1102			Danio		Fish		Author reported half the detection limit
	Wood frog Rana sylvatica		115		469		16		MATC-survival		gt		2000		gt		9746		gt		9746		9746		gt		Rana		Amphib - Other Data		Value is from 2007 FW Cu AWQC Appendix C recommendation		aged tap water

	Appendix A Acceptable Acute Toxicity Data of Aluminum to Freshwater Aquatic Animals
	Species		Methoda		Hardness (mgL as CaCO3)		pH		DOC(mgL)			LC50 or EC50 Conc (microgL)		Reference			Normalized Conc - LC50(microgL)			Species Mean Acute Value(microgL)		Genus Mean Acute Value(microgL)			Genus			DOC Note		Dilution water
	Snail Physa sp		S M T		474		659		11		gt		23400		Call 1984 Call et al 1984		gt		48425			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		755		11			30600		Call 1984 Call et al 1984			26986			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		817		11		gt		24700		Call 1984 Call et al 1984		gt		24040			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		746		11			55500		Call 1984 Call et al 1984			50338			35462		35462			Physa		Mollusk		Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Fatmucket Lampsilis siliquoidea		F M T		1060		612		048		gt		6302		Wang et al 2016 Wang et al 2017		gt		29834		gt		29834		29834		gt		Lampsilis		Mollusk		Author reported
	Cladoceran Ceriodaphnia dubia		S M A		500		742		11			1900		McCauley et al 1986			1728			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		S M A		505		786		11			1500		McCauley et al 1986			1294			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		UW-S Lake Superior lab water
	Cladoceran Ceriodaphnia dubia		S M A		500		813		11			2560		McCauley et al 1986			2431			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		R M T		26		75		05			720		ENSR 1992d			1113			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		R M T		46		76		05			1880		ENSR 1992d			2495			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		R M T		96		78		05			2450		ENSR 1992d			2894			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		S U NR		90		715		05			3727		Fort and Stover 1995			5002			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U NR		90		715		05			5673		Fort and Stover 1995			7613			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U NR		89		76		05			2880		Soucek et al 2001			3362			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		601		05			7112		European Al Association 2009			1691			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		605		2			6865		European Al Association 2009			7088			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		609		4			15581		European Al Association 2009			10072			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		601		05			681		European Al Association 2009			1620			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		603		05			1630		European Al Association 2009			3674			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		597		05			1785		European Al Association 2009			4732			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		592		05			1410		European Al Association 2009			4295			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		699		05		gt		1300		European Al Association 2009		gt		4189			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		785		05		gt		5000		European Al Association 2009		gt		7960			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		68		2		gt		10000		European Al Association 2009		gt		20755			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		782		2		gt		15000		European Al Association 2009		gt		11626			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		677		4		gt		10000		European Al Association 2009		gt		15161			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		766		4		gt		15000		European Al Association 2009		gt		8608			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		79		05		gt		2000		European Al Association 2009		gt		3150			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		789		05		gt		2000		European Al Association 2009		gt		3156			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		604		05			1108		European Al Association 2009			850			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		598		2			11371		European Al Association 2009			4817			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		573		4			80467		European Al Association 2009			43570			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		671		05		gt		10000		European Al Association 2009		gt		23786			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		783		05		gt		5000		European Al Association 2009		gt		6344			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		679		2		gt		10000		European Al Association 2009		gt		10394			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		767		2		gt		15000		European Al Association 2009		gt		9082			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		668		4		gt		15000		European Al Association 2009		gt		12461			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		762		4		gt		15000		European Al Association 2009		gt		6332			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		606		2			33868		European Al Association 2009			7920			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		56		4			104842		European Al Association 2009			48800			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		693		05		gt		5000		European Al Association 2009		gt		7126			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		120		788		05		gt		5000		European Al Association 2009		gt		5898			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		120		676		2		gt		15000		European Al Association 2009		gt		12117			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		771		2		gt		15000		European Al Association 2009		gt		8096			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		66		4		gt		15000		European Al Association 2009		gt		10139			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		76		4		gt		15000		European Al Association 2009		gt		5545			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			11971		European Al Association 2010			2699			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			27478		European Al Association 2010			6194			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			11998		European Al Association 2010			2705			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		607		05			92495		European Al Association 2010			1876			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		609		05			31337		European Al Association 2010			6035			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		61		05			33235		European Al Association 2010			6238			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		708		05		gt		8864		European Al Association 2010		gt		2534			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		779		05		gt		42783		European Al Association 2010		gt		6930			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		753		05			13204		European Al Association 2010			244			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		600		601		05			46326		European Al Association 2010			3798			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		600		599		05		gt		8590		European Al Association 2010		gt		7366			5094				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia reticulata		F M T		451		60		11			304		Shephard 1983			2009			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia reticulata		F M T		40		55		11			362		Shephard 1983			55307			10542		7328			Ceriodaphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake SuperiorDI Mix
	Cladoceran Daphnia magna		S U NR		453		774		11			3900		Biesinger and Christensen 1972			3382			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Daphnia magna		S M T		220		76		16			38200		Kimball 1978			20303			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Well water
	Cladoceran Daphnia magna		S U T		451		725		11			2800		Shephard 1983			2868			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Daphnia magna		S U T		168		599		05			5000		European Al Association 2009		gt		2265			-				Daphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Daphnia magna		S U T		168		792		05			7950		European Al Association 2009			920			3332		3332			Daphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Ostracod Stenocypris major		R M T		1563		651		16			310196		Shuhaimi-Othman et al 2011a			10216			10216		10216			Stenocypris		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (Malaysia)
	Amphipod Crangonyx pseudogracilis		R U T		50		675		16			9190		Martin and Holdich 1986			12174			12174		12174			Crangonyx		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (UK)
	Amphipod Hyalella azteca		F M T		105		613		048		gt		5997		Wang et al 2016 Wang et al 2017		gt		28002		gt		28002		28002		gt		Hyalella		Invert		Author reported
	Stonefly Acroneuria sp		S M T		474		746		11		gt		22600		Call et al 1984		gt		20498		gt		20498		20498		gt		Acroneuria		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Midge Chironomus plumosus		S U T		80		70		16			30000		Fargasova 2001			25361			25361		25361			Chironomus		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (Slovakia)
	Midge Paratanytarsus dissimilis		S M T		1743		728		28		gt		77700		Lamb and Bailey 1981 1983		gt		62318		gt		62318		62318		gt		Paratanytarsus		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Liberty Lake Washington
	Rainbow trout Oncorhynchus mykiss		F M T		274		758		05		gt		9840		Gundersen et al 1994		gt		5745			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		452		762		05		gt		8070		Gundersen et al 1994		gt		5212			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		895		758		05		gt		8160		Gundersen et al 1994		gt		6807			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		1304		758		05		gt		8200		Gundersen et al 1994		gt		7662			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		232		825		05			6170		Gundersen et al 1994			1190			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		350		826		05			6170		Gundersen et al 1994			1523			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		836		829		05			7670		Gundersen et al 1994			3205			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		1158		830		05			6930		Gundersen et al 1994			3554			3661		3661			Oncorhynchus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Atlantic salmon Salmo salar		S U T		68		55		05			584		Hamilton and Haines 1995			21042			-				Salmo			Value is from 2007 FW Cu AWQC Appendix C recommendation		Soft recon
	Atlantic salmon Salmo salar		S U T		68		65		05			599		Hamilton and Haines 1995			2430			7151		7151			Salmo		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Soft recon
	Brook trout Salvelinus fontinalis		S U T		40		56		16			6530		Tandjung 1982			31767			-				Salvelinus			Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Brook trout Salvelinus fontinalis		S U T		18		56		16			3400		Tandjung 1982			28391			-				Salvelinus			Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Brook trout Salvelinus fontinalis		S U T		2		56		16			370		Tandjung 1982			13663			23097		23097			Salvelinus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Green sunfish Lepomis cyanellus		S M T		474		755		11		gt		50000		Call et al 1984		gt		24028		gt		24028		24028		gt		Lepomis		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Rio Grande silvery minnow Hybognathus amarus		R M T		140		81		05		gt		59100		Buhl 2002		gt		39414		gt		39414		39414		gt		Hybognathus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon water
	Fathead minnow Pimephales promelas		S M T		474		761		11		gt		48200		Call et al 1984		gt		21522			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Fathead minnow Pimephales promelas		S M T		474		805		11		gt		49800		Call et al 1984		gt		12972			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Fathead minnow Pimephales promelas		R M T		26		78		05			1160		ENSR 1992c			477			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		46		76		05			8180		ENSR 1992c			5446			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		96		81		05			20300		ENSR 1992c			10966			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		194		81		05			44800		ENSR 1992c			35847			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		140		81		05		gt		59100		Buhl 2002		gt		39414			10168		10168			Pimephales		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon water
	Smallmouth bass Micropterus dolomieui		S M T		1245		505		16			130		Kane and Rabeni 1987			3929			3929		3929			Micropterus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Green tree frog Hyla cinerea		R M T		455		549		05		gt		4052		Jung and Jagoe 1995		gt		20016		gt		20016		20016		gt		Hyla		Amphibian		Value is from 2007 FW Cu AWQC Appendix C recommendation		artificial soft recon

	Normalization Chemistry
	pH		7					FAV		2741
	Hardness		100					CMC		1400
	DOC		1					CCC		390
	Acute Ranked GMAV											Chronic Ranked GMCV
	Rank			GMAV		Genus								Rank			GMCV		Genus
	18		gt		62318		Paratanytarsus		Invert							12			17098		Aeolosoma		Invert
	17		gt		39414		Hybognathus		Fish							11		gt		9746		Rana		Amphib - Other Data
	16			35462		Physa		Mollusk							10			5908		Chironomus		Invert
	15		gt		29834		Lampsilis		Mollusk							9			4877		Lymnaea		Mollusk
	14		gt		28002		Hyalella		Invert							8			2555		Brachionus		Invert
	13			25361		Chironomus		Invert							7			2488		Pimephales		Fish
	12		gt		24028		Lepomis		Fish							6			1713		Hyalella		Invert
	11			23097		Salvelinus		Fish							5			1182		Ceriodaphnia		Invert
	10		gt		20498		Acroneuria		Invert							4			1102		Danio		Fish
	9		gt		20016		Hyla		Amphibian							3			1042		Lampsilis		Mollusk
	8			12174		Crangonyx		Invert							2			591		Salvelinus		Fish
	7			10216		Stenocypris		Invert							1			509		Salmo		Fish
	6			10168		Pimephales		Fish
	5			7328		Ceriodaphnia		Invert
	4			7151		Salmo		Fish
	3			3929		Micropterus		Fish
	2			3661		Oncorhynchus		Fish
	1			3332		Daphnia		Invert
		N		Rank		Genus		GMAV		ln(GMAV)		ln(GMAV)2		P=R(N+1)		sqrt(P)				N		Rank		Genus		GMCV		ln(GMCV)		ln(GMCV)2		P=R(N+1)		sqrt(P)
		18		4		Salmo		7151		887		7876		0211		0459				12		4		Danio		1102		700		4907		0308		0555
			3		Micropterus		3929		828		6849		0158		0397					3		Lampsilis		1042		695		4829		0231		0480
			2		Oncorhynchus		3661		821		6733		0105		0324					2		Salvelinus		591		638		4074		0154		0392
			1		Daphnia		3332		811		6579		0053		0229					1		Salmo		509		623		3883		0077		0277
			Sum				3347		2804		0526		141					Sum				2657		1769		0769		170
			S2 =		1223										S2 =		1080
			L =		7134										L =		5242
			A =		7916										A =		5976
			FAV =		2741										FCV =		3940
			CMC =		1371

				All concentrations reported are microgL total Aluminum
				lt-----Click Button after entering data
	ENTER DATA HERE									ERRORNUM		ACUTE														CHRONIC
										Rank 1				Rank 2				Rank 3				Rank 4					Rank 1				Rank 2				Rank 3				Rank 4
	SiteName		DOC(mgL)		Hardness(mgL as CaCO3)		pH			FAV		CMC		CCC			Genus		GMAV			Genus		GMAV			Genus		GMAV			Genus		GMAV				Genus		GMCV			Genus		GMCV			Genus		GMCV			Genus		GMCV
	Site 1		50		25		9		75428737034162		35229324666008		1800		770			Daphnia			33452		Ceriodaphnia			73576		Stenocypris			102576		Crangonyx			122230			Lampsilis			10463		Ceriodaphnia			11865		Hyalella			17200		Brachionus			25648
	Site 2		50		50		9		75428737034162		31204991509063		1600		680			Daphnia			29631		Ceriodaphnia			65171		Stenocypris			90859		Crangonyx			108267			Lampsilis			9268		Ceriodaphnia			10510		Hyalella			15236		Brachionus			22718
	Site 3		50		75		9		75428737034162		29067527928199		1500		640			Daphnia			27601		Ceriodaphnia			60707		Stenocypris			84635		Crangonyx			100851			Lampsilis			8633		Ceriodaphnia			9790		Hyalella			14192		Brachionus			21162
	Site 4		50		100		9		75428737034162		27640367912592		1400		600			Daphnia			26246		Ceriodaphnia			57726		Stenocypris			80480		Crangonyx			95899			Lampsilis			8209		Ceriodaphnia			9309		Hyalella			13495		Brachionus			20123
	Site 5		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 6		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 7		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 8		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 9		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 10		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 11		50		25		85		75428737034162		63245275664384		3200		1400			Daphnia			60055		Ceriodaphnia			132086		Stenocypris			184149		Crangonyx			219432			Lampsilis			18783		Ceriodaphnia			21301		Hyalella			30879		Brachionus			46045
	Site 12		50		50		85		75428737034162		61388044609926		3100		1300			Daphnia			58291		Ceriodaphnia			128208		Stenocypris			178742		Crangonyx			212988			Lampsilis			18232		Ceriodaphnia			20676		Hyalella			29972		Brachionus			44693
	Site 13		50		75		85		75428737034162		60327020429132		3000		1300			Daphnia			57284		Ceriodaphnia			125992		Stenocypris			175652		Crangonyx			209307			Lampsilis			17917		Ceriodaphnia			20318		Hyalella			29454		Brachionus			43920
	Site 14		50		100		85		75428737034162		59585352130146		3000		1300			Daphnia			56580		Ceriodaphnia			124443		Stenocypris			173493		Crangonyx			206734			Lampsilis			17696		Ceriodaphnia			20068		Hyalella			29092		Salmo			41264
	Site 15		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 16		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 17		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 18		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 19		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 20		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370

III Normalize toxicity data in species sensitivity distribution to a set of water

quality conditionsbull Just like single parameter hardness models the MLR

models were used to normalize the aluminum toxicity data to a single set of water quality conditionsndash As an illustration for one set of calculations the draft

aluminum AWQC set these conditions to pH 7 hardness of 100 mgL and DOC of 1 mgL

bull This allows for comparisons to be made between species based on their sensitivity to aluminumndash Apples to apples

20

IV Construct sensitivity distribution to determine criteria at set conditions

21

Figure 8 Ranked Summary of Total Aluminum Genus Mean Acute Values (GMAVs) - Freshwater at pH 7 Hardness of 100 mgL and DOC of 1 mgL

IV Construct sensitivity distribution to determine criteria at set conditions

22

Figure 10 Ranked Summary of Total Aluminum Genus Mean Chronic Values (GMCVs) ndash Freshwater Supplemented with Other Data to Fulfill Missing MDRs at pH 7 Hardness of 100 mgL and DOC of 1 mgL

Aluminum Criteria Calculatorbull The relative ranking of genera in the sensitivity

distribution to which the toxicity data are normalized will change based on the set of water chemistry conditions (ie pH hardness and DOC)

bull To make calculating aluminum criteria easier EPA developed an Aluminum Criteria Calculatorndash Automated so that user only needs to enter water chemistry

conditions of interestndash Allows quick calculations for a variety of conditionsndash Limits calculations to the recommended conditions for which

the MLR models were developed were specified for hardness and DOC

bull No extrapolation beyond hardness and DOC empirical data bounds

23

Aluminum Criteria Calculator SD Example

24

pH=7 Hardness=100 DOC=1 pH=6 Hardness=25 DOC=1

Chronic Ranked GMCVRank GMCV Genus

12 17098 Aeolosoma Invert11 gt 9746 Rana Amphib - Other Data10 5908 Chironomus Invert

9 4877 Lymnaea Mollusk8 2555 Brachionus Invert7 2488 Pimephales Fish6 1713 Hyalella Invert5 1182 Ceriodaphnia Invert4 1102 Danio Fish3 1042 Lampsilis Mollusk2 591 Salvelinus Fish1 509 Salmo Fish

FCV = 3940 microgL total aluminum

Chronic Ranked GMCVRank GMCV Genus

12 gt 2131 Rana Amphib - Other Data11 1710 Aeolosoma Invert10 591 Chironomus Invert

9 544 Pimephales Fish8 488 Lymnaea Mollusk7 255 Brachionus Invert6 241 Danio Fish5 171 Hyalella Invert4 129 Salvelinus Fish3 118 Ceriodaphnia Invert2 111 Salmo Fish1 104 Lampsilis Mollusk

FCV = 987 microgL total aluminum

Multiple Scenarios

Run Scenarios

Summary Sheet

Acute Dataset

Chronic Dataset

Multiple Scenarios

Run Scenarios

Summary Sheet

Acute Dataset

Chronic Dataset

Boundaries of the Criteria Outputsbull MLR models were developed using data that

encompassndash DOC range of 008-5 mgLndash Hardness range of 98-127 mgL (as CaCO3)ndash pH range of 6-81

bull MLR criteria outputs are bounded atndash Maximum of 150 mgL total hardness (as CaCO3)

ndash DOC of 50 mgLndash pH range of the model is 50 to 90

25

Aluminum Criteria Look-Up Tables

26

bull EPA also provided criteria look-up tables in Appendix K

Har

dnes

s

CCC(microgL total aluminum)

(DOC=05 mgL)

pH 50 pH 55 pH 60 pH 65 pH 70 pH 75 pH 80 pH 85 pH 90

25 23 a 14 b 69 c 160 f 350 f 620 i 540 b 410 a 230 a

50 43 a 24 b 100 d 170 f 310 f 630 f 670 j 400 a 200 a

75 61 a 33 b 130 e 180 f 290 f 530 f 730 i 390 a 190 a

100 78 a 41 b 150 e 190 f 280 g 460 f 770 c 390 a 180 a

150 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

200 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

250 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

300 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

350 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

400 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

Aluminum Criteria Look-Up Tables

27

bull EPA also provided criteria look-up tables in Appendix K

Har

dnes

s

CCC(microgL total aluminum)

(DOC=10 mgL)

pH 50 pH 55 pH 60 pH 65 pH 70 pH 75 pH 80 pH 85 pH 90

25 33 a 21 b 99 c 220 f 490 f 900 i 780 b 590 a 330 a

50 61 a 35 b 150 e 250 f 440 f 890 f 960 j 580 a 290 a

75 88 a 47 b 180 e 260 f 410 f 740 f 1100 i 570 a 270 a

100 11 a 59 b 210 e 270 f 390 g 650 f 1100 c 560 b 260 a

150 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

200 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

250 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

300 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

350 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

400 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

Aluminum Criteria Look-Up Tables

28

bull EPA also provided criteria look-up tables in Appendix K

Har

dnes

s

CCC(microgL total aluminum)

(DOC=25 mgL)

pH 50 pH 55 pH 60 pH 65 pH 70 pH 75 pH 80 pH 85 pH 90

25 54 a 34 b 160 c 350 f 770 f 1500 i 1300 b 960 a 540 a

50 99 a 57 b 230 e 390 f 690 f 1400 f 1600 j 930 a 470 a

75 14 a 77 b 290 e 410 f 640 f 1200 f 1700 i 920 a 440 a

100 18 a 96 b 330 e 420 f 620 g 1000 f 1800 d 920 b 420 a

150 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

200 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

250 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

300 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

350 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

400 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

Aluminum Criteria Look-Up Tables

29

bull EPA also provided criteria look-up tables in Appendix K

Har

dnes

s

CCC(microgL total aluminum)

(DOC=50 mgL)

pH 50 pH 55 pH 60 pH 65 pH 70 pH 75 pH 80 pH 85 pH 90

25 77 a 49 b 230 c 500 f 1100 f 2100 c 1900 b 1400 a 770 a

50 14 a 82 b 330 e 540 f 970 f 2000 f 2200 j 1300 a 680 a

75 20 a 110 b 410 e 570 f 900 f 1600 f 2500 i 1300 a 640 a

100 26 a 140 b 460 f 590 f 880 g 1400 f 2500 d 1300 b 600 a

150 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

200 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

250 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

300 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

350 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

400 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

Status

30

bull Draft Document Released to the Federal Register on 72817ndash Public comment period of 60 days ndash Due Date for Comments is 92617

bull Revisions will be made considering public commentsbull Subsequent EPA intra-agency review of final criteria

document will then be conducted bull Publication of final criteria document planned for Late Fall

2017

Federal Register Webpagehttpswwwfederalregistergovdocuments201

707282017-15968request-for-scientific-views-draft-updated-aquatic-life-ambient-water-quality-criteria-for-aluminum

Aluminum Criteria Webpagehttpswwwepagovwqc2017-draft-aquatic-life-

criteria-aluminum-freshwater-documents

31

	Appendix C Acceptable Chronic Toxicity Data of Aluminum to Freshwater Aquatic Animals
									MLR equation
	Species		Hardness (mgL as CaCO3)		pH		DOC(mgL)		EC20 Endpoint			EC20(microgL)			Normalized Conc- EC20(microgL)			SMCV-EC20(microgL)		GMCV-EC20(microgL)			Genus			DOC Notes		Dilution water
	Oligochaete Aeolosoma sp		48		6		025		Reproduction (population size)			1235			1710			1710		1710			Aeolosoma		Invert		Author reported half the detection limit
	Rotifer Brachionus calyciflorus		100		62		025		Reproduction (population size)			431			255			255		255			Brachionus		Invert		Author reported half the detection limit
	Great pond snail Lymnaea stagnalis		117		61		025		Biomass			746			488			488		488			Lymnaea		Mollusk		Author reported half the detection limit
	Fatmucket Lampsilis siliquoidea		1055		604		040		Biomass			169			104			104		104			Lampsilis		Mollusk		Author reported
	Cladoceran Ceriodaphnia dubia		50		715		11		Reproduction - youngstarting adult			1780			190						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		26		73		05		Reproduction - youngfemale			1557			272						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		46		75		05		Reproduction - youngfemale			8087			110						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		96		79		05		Reproduction - youngfemale			6519			80						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		194		81		05		Reproduction - youngfemale			6836			89						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		255		703		05		Reproduction (youngfemale)			250			56						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		122		713		05		Reproduction (youngfemale)			860			106						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		25		798		05		Reproduction (youngfemale)			700			101						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		61		803		05		Reproduction (youngfemale)			1010			136						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		122		810		05		Reproduction (youngfemale)			870			116						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		255		633		05		Reproduction (youngfemale)			260			176						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		121		636		05		Reproduction (youngfemale)			390			110			118		118			Ceriodaphnia		Invert		Author reported
	Amphipod Hyalella azteca		95		61		051		Biomass (28 d)			1993			101						Hyalella			Author reported
	Amphipod Hyalella azteca		106		604		033		Biomass (28 d)			425			289			171		171			Hyalella		Invert		Author reported
	Midge Chironomus riparius		118		558		18		Adult midge emergence			2955			119						Chironomus			Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Midge Chironomus riparius		119		505		18		Adult midge emergence			8442			2258						Chironomus			Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Midge Chironomus riparius		91		66		051		Reproduction ( of eggscase)			3387			766			591		591			Chironomus		Invert		Author reported
	Atlantic salmon Salmo salar		127		57		18		Biomass			6156			1112			1112		1112			Salmo		Fish		Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Brook trout Salvelinus fontinalis		123		655		19		Biomass			1644			59						Salvelinus			Author reported		wellRO mix
	Brook trout Salvelinus fontinalis		128		565		18		Biomass			1435			283			1293		1293			Salvelinus		Fish		Author reported		wellRO mix
	Fathead minnow Pimephales promelas		220		770		16		Biomass			6194			781						Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		well water
	Fathead minnow Pimephales promelas		96		620		025		Fry survival			4286			379			544		544			Pimephales		Fish		Author reported half the detection limit
	Zebrafish Danio rerio		83		61		025		Biomass			2344			241			241		241			Danio		Fish		Author reported half the detection limit
	Wood frog Rana sylvatica		115		469		16		MATC-survival		gt		2000		gt		2131		gt		2131		2131		gt		Rana		Amphib - Other Data		Value is from 2007 FW Cu AWQC Appendix C recommendation		aged tap water

	Appendix A Acceptable Acute Toxicity Data of Aluminum to Freshwater Aquatic Animals
	Species		Methoda		Hardness (mgL as CaCO3)		pH		DOC(mgL)			LC50 or EC50 Conc (microgL)		Reference			Normalized Conc - LC50(microgL)			Species Mean Acute Value(microgL)		Genus Mean Acute Value(microgL)			Genus			DOC Note		Dilution water
	Snail Physa sp		S M T		474		659		11		gt		23400		Call 1984 Call et al 1984		gt		4843			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		755		11			30600		Call 1984 Call et al 1984			2699			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		817		11		gt		24700		Call 1984 Call et al 1984		gt		2404			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		746		11			55500		Call 1984 Call et al 1984			5034			3546		3546			Physa		Mollusk		Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Fatmucket Lampsilis siliquoidea		F M T		1060		612		048		gt		6302		Wang et al 2016 Wang et al 2017		gt		2983		gt		2983		2983		gt		Lampsilis		Mollusk		Author reported
	Cladoceran Ceriodaphnia dubia		S M A		500		742		11			1900		McCauley et al 1986			173			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		S M A		505		786		11			1500		McCauley et al 1986			129			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		UW-S Lake Superior lab water
	Cladoceran Ceriodaphnia dubia		S M A		500		813		11			2560		McCauley et al 1986			243			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		R M T		26		75		05			720		ENSR 1992d			111			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		R M T		46		76		05			1880		ENSR 1992d			250			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		R M T		96		78		05			2450		ENSR 1992d			289			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		S U NR		90		715		05			3727		Fort and Stover 1995			500			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U NR		90		715		05			5673		Fort and Stover 1995			761			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U NR		89		76		05			2880		Soucek et al 2001			336			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		601		05			7112		European Al Association 2009			169			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		605		2			6865		European Al Association 2009			709			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		609		4			15581		European Al Association 2009			1007			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		601		05			681		European Al Association 2009			162			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		603		05			1630		European Al Association 2009			367			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		597		05			1785		European Al Association 2009			473			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		592		05			1410		European Al Association 2009			429			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		699		05		gt		1300		European Al Association 2009		gt		419			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		785		05		gt		5000		European Al Association 2009		gt		796			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		68		2		gt		10000		European Al Association 2009		gt		2076			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		782		2		gt		15000		European Al Association 2009		gt		1163			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		677		4		gt		10000		European Al Association 2009		gt		1516			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		766		4		gt		15000		European Al Association 2009		gt		861			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		79		05		gt		2000		European Al Association 2009		gt		315			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		789		05		gt		2000		European Al Association 2009		gt		316			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		604		05			1108		European Al Association 2009			85			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		598		2			11371		European Al Association 2009			482			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		573		4			80467		European Al Association 2009			4357			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		671		05		gt		10000		European Al Association 2009		gt		2379			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		783		05		gt		5000		European Al Association 2009		gt		634			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		679		2		gt		10000		European Al Association 2009		gt		1039			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		767		2		gt		15000		European Al Association 2009		gt		908			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		668		4		gt		15000		European Al Association 2009		gt		1246			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		762		4		gt		15000		European Al Association 2009		gt		633			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		606		2			33868		European Al Association 2009			792			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		56		4			104842		European Al Association 2009			4880			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		693		05		gt		5000		European Al Association 2009		gt		713			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		120		788		05		gt		5000		European Al Association 2009		gt		590			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		120		676		2		gt		15000		European Al Association 2009		gt		1212			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		771		2		gt		15000		European Al Association 2009		gt		810			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		66		4		gt		15000		European Al Association 2009		gt		1014			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		76		4		gt		15000		European Al Association 2009		gt		555			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			11971		European Al Association 2010			270			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			27478		European Al Association 2010			619			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			11998		European Al Association 2010			270			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		607		05			92495		European Al Association 2010			188			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		609		05			31337		European Al Association 2010			604			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		61		05			33235		European Al Association 2010			624			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		708		05		gt		8864		European Al Association 2010		gt		253			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		779		05		gt		42783		European Al Association 2010		gt		693			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		753		05			13204		European Al Association 2010			24			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		600		601		05			46326		European Al Association 2010			380			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		600		599		05		gt		8590		European Al Association 2010		gt		737			509				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia reticulata		F M T		451		60		11			304		Shephard 1983			201			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia reticulata		F M T		40		55		11			362		Shephard 1983			5531			1054		733			Ceriodaphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake SuperiorDI Mix
	Cladoceran Daphnia magna		S U NR		453		774		11			3900		Biesinger and Christensen 1972			338			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Daphnia magna		S M T		220		76		16			38200		Kimball 1978			2030			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Well water
	Cladoceran Daphnia magna		S U T		451		725		11			2800		Shephard 1983			287			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Daphnia magna		S U T		168		599		05			5000		European Al Association 2009		gt		227			-				Daphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Daphnia magna		S U T		168		792		05			7950		European Al Association 2009			92			333		333			Daphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Ostracod Stenocypris major		R M T		1563		651		16			310196		Shuhaimi-Othman et al 2011a			1022			1022		1022			Stenocypris		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (Malaysia)
	Amphipod Crangonyx pseudogracilis		R U T		50		675		16			9190		Martin and Holdich 1986			1217			1217		1217			Crangonyx		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (UK)
	Amphipod Hyalella azteca		F M T		105		613		048		gt		5997		Wang et al 2016 Wang et al 2017		gt		2800		gt		2800		2800		gt		Hyalella		Invert		Author reported
	Stonefly Acroneuria sp		S M T		474		746		11		gt		22600		Call et al 1984		gt		2050		gt		2050		2050		gt		Acroneuria		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Midge Chironomus plumosus		S U T		80		70		16			30000		Fargasova 2001			2536			2536		2536			Chironomus		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (Slovakia)
	Midge Paratanytarsus dissimilis		S M T		1743		728		28		gt		77700		Lamb and Bailey 1981 1983		gt		6232		gt		6232		6232		gt		Paratanytarsus		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Liberty Lake Washington
	Rainbow trout Oncorhynchus mykiss		F M T		274		758		05		gt		9840		Gundersen et al 1994		gt		1256			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		452		762		05		gt		8070		Gundersen et al 1994		gt		1140			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		895		758		05		gt		8160		Gundersen et al 1994		gt		1489			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		1304		758		05		gt		8200		Gundersen et al 1994		gt		1676			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		232		825		05			6170		Gundersen et al 1994			260			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		350		826		05			6170		Gundersen et al 1994			333			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		836		829		05			7670		Gundersen et al 1994			701			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		1158		830		05			6930		Gundersen et al 1994			777			801		801			Oncorhynchus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Atlantic salmon Salmo salar		S U T		68		55		05			584		Hamilton and Haines 1995			4602			-				Salmo			Value is from 2007 FW Cu AWQC Appendix C recommendation		Soft recon
	Atlantic salmon Salmo salar		S U T		68		65		05			599		Hamilton and Haines 1995			531			1564		1564			Salmo		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Soft recon
	Brook trout Salvelinus fontinalis		S U T		40		56		16			6530		Tandjung 1982			6947			-				Salvelinus			Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Brook trout Salvelinus fontinalis		S U T		18		56		16			3400		Tandjung 1982			6209			-				Salvelinus			Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Brook trout Salvelinus fontinalis		S U T		2		56		16			370		Tandjung 1982			2988			5051		5051			Salvelinus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Green sunfish Lepomis cyanellus		S M T		474		755		11		gt		50000		Call et al 1984		gt		5255		gt		5255		5255		gt		Lepomis		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Rio Grande silvery minnow Hybognathus amarus		R M T		140		81		05		gt		59100		Buhl 2002		gt		8620		gt		8620		8620		gt		Hybognathus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon water
	Fathead minnow Pimephales promelas		S M T		474		761		11		gt		48200		Call et al 1984		gt		4707			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Fathead minnow Pimephales promelas		S M T		474		805		11		gt		49800		Call et al 1984		gt		2837			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Fathead minnow Pimephales promelas		R M T		26		78		05			1160		ENSR 1992c			104			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		46		76		05			8180		ENSR 1992c			1191			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		96		81		05			20300		ENSR 1992c			2398			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		194		81		05			44800		ENSR 1992c			7839			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		140		81		05		gt		59100		Buhl 2002		gt		8620			2224		2224			Pimephales		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon water
	Smallmouth bass Micropterus dolomieui		S M T		1245		505		16			130		Kane and Rabeni 1987			859			859		859			Micropterus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Green tree frog Hyla cinerea		R M T		455		549		05		gt		4052		Jung and Jagoe 1995		gt		4377		gt		4377		4377		gt		Hyla		Amphibian		Value is from 2007 FW Cu AWQC Appendix C recommendation		artificial soft recon

	Normalization Chemistry
	pH		6					FAV		360
	Hardness		25					CMC		180
	DOC		1					CCC		99
	Acute Ranked GMAV											Chronic Ranked GMCV
	Rank			GMAV		Genus								Rank			GMCV		Genus
	18		gt		8620		Hybognathus		Fish							12		gt		2131		Rana		Amphib - Other Data
	17		gt		6232		Paratanytarsus		Invert							11			1710		Aeolosoma		Invert
	16		gt		5255		Lepomis		Fish							10			591		Chironomus		Invert
	15			5051		Salvelinus		Fish							9			544		Pimephales		Fish
	14		gt		4377		Hyla		Amphibian							8			488		Lymnaea		Mollusk
	13			3546		Physa		Mollusk							7			255		Brachionus		Invert
	12		gt		2983		Lampsilis		Mollusk							6			241		Danio		Fish
	11		gt		2800		Hyalella		Invert							5			171		Hyalella		Invert
	10			2536		Chironomus		Invert							4			129		Salvelinus		Fish
	9			2224		Pimephales		Fish							3			118		Ceriodaphnia		Invert
	8		gt		2050		Acroneuria		Invert							2			111		Salmo		Fish
	7			1564		Salmo		Fish							1			104		Lampsilis		Mollusk
	6			1217		Crangonyx		Invert
	5			1022		Stenocypris		Invert
	4			859		Micropterus		Fish
	3			801		Oncorhynchus		Fish
	2			733		Ceriodaphnia		Invert
	1			333		Daphnia		Invert
		N		Rank		Genus		GMAV		ln(GMAV)		ln(GMAV)2		P=R(N+1)		sqrt(P)				N		Rank		Genus		GMCV		ln(GMCV)		ln(GMCV)2		P=R(N+1)		sqrt(P)
		18		4		Micropterus		859		676		4564		0211		0459				12		4		Salvelinus		129		486		2364		0308		0555
			3		Oncorhynchus		801		669		4469		0158		0397					3		Ceriodaphnia		118		477		2277		0231		0480
			2		Ceriodaphnia		733		660		4352		0105		0324					2		Salmo		111		471		2220		0154		0392
			1		Daphnia		333		581		3374		0053		0229					1		Lampsilis		104		465		2159		0077		0277
			Sum				2585		1676		0526		141					Sum				1899		902		0769		170
			S2 =		1987										S2 =		059
			L =		4890										L =		4420
			A =		5887										A =		4592
			FAV =		360										FCV =		987
			CMC =		180

				All concentrations reported are microgL total Aluminum
				lt-----Click Button after entering data
	ENTER DATA HERE									ERRORNUM		ACUTE														CHRONIC
										Rank 1				Rank 2				Rank 3				Rank 4					Rank 1				Rank 2				Rank 3				Rank 4
	SiteName		DOC(mgL)		Hardness(mgL as CaCO3)		pH			FAV		CMC		CCC			Genus		GMAV			Genus		GMAV			Genus		GMAV			Genus		GMAV				Genus		GMCV			Genus		GMCV			Genus		GMCV			Genus		GMCV
	Site 1		50		25		9		75428737034162		35229324666008		1800		770			Daphnia			33452		Ceriodaphnia			73576		Stenocypris			102576		Crangonyx			122230			Lampsilis			10463		Ceriodaphnia			11865		Hyalella			17200		Brachionus			25648
	Site 2		50		50		9		75428737034162		31204991509063		1600		680			Daphnia			29631		Ceriodaphnia			65171		Stenocypris			90859		Crangonyx			108267			Lampsilis			9268		Ceriodaphnia			10510		Hyalella			15236		Brachionus			22718
	Site 3		50		75		9		75428737034162		29067527928199		1500		640			Daphnia			27601		Ceriodaphnia			60707		Stenocypris			84635		Crangonyx			100851			Lampsilis			8633		Ceriodaphnia			9790		Hyalella			14192		Brachionus			21162
	Site 4		50		100		9		75428737034162		27640367912592		1400		600			Daphnia			26246		Ceriodaphnia			57726		Stenocypris			80480		Crangonyx			95899			Lampsilis			8209		Ceriodaphnia			9309		Hyalella			13495		Brachionus			20123
	Site 5		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 6		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 7		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 8		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 9		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 10		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 11		50		25		85		75428737034162		63245275664384		3200		1400			Daphnia			60055		Ceriodaphnia			132086		Stenocypris			184149		Crangonyx			219432			Lampsilis			18783		Ceriodaphnia			21301		Hyalella			30879		Brachionus			46045
	Site 12		50		50		85		75428737034162		61388044609926		3100		1300			Daphnia			58291		Ceriodaphnia			128208		Stenocypris			178742		Crangonyx			212988			Lampsilis			18232		Ceriodaphnia			20676		Hyalella			29972		Brachionus			44693
	Site 13		50		75		85		75428737034162		60327020429132		3000		1300			Daphnia			57284		Ceriodaphnia			125992		Stenocypris			175652		Crangonyx			209307			Lampsilis			17917		Ceriodaphnia			20318		Hyalella			29454		Brachionus			43920
	Site 14		50		100		85		75428737034162		59585352130146		3000		1300			Daphnia			56580		Ceriodaphnia			124443		Stenocypris			173493		Crangonyx			206734			Lampsilis			17696		Ceriodaphnia			20068		Hyalella			29092		Salmo			41264
	Site 15		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 16		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 17		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 18		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 19		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 20		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370

	Appendix C Acceptable Chronic Toxicity Data of Aluminum to Freshwater Aquatic Animals
									MLR equation
	Species		Hardness (mgL as CaCO3)		pH		DOC(mgL)		EC20 Endpoint			EC20(microgL)			Normalized Conc- EC20(microgL)			SMCV-EC20(microgL)		GMCV-EC20(microgL)			Genus			DOC Notes		Dilution water
	Oligochaete Aeolosoma sp		48		6		025		Reproduction (population size)			1235			17098			17098		17098			Aeolosoma		Invert		Author reported half the detection limit
	Rotifer Brachionus calyciflorus		100		62		025		Reproduction (population size)			431			2555			2555		2555			Brachionus		Invert		Author reported half the detection limit
	Great pond snail Lymnaea stagnalis		117		61		025		Biomass			746			4877			4877		4877			Lymnaea		Mollusk		Author reported half the detection limit
	Fatmucket Lampsilis siliquoidea		1055		604		040		Biomass			169			1042			1042		1042			Lampsilis		Mollusk		Author reported
	Cladoceran Ceriodaphnia dubia		50		715		11		Reproduction - youngstarting adult			1780			1898						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		26		73		05		Reproduction - youngfemale			1557			2718						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		46		75		05		Reproduction - youngfemale			8087			1102						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		96		79		05		Reproduction - youngfemale			6519			796						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		194		81		05		Reproduction - youngfemale			6836			888						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		255		703		05		Reproduction (youngfemale)			250			563						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		122		713		05		Reproduction (youngfemale)			860			1061						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		25		798		05		Reproduction (youngfemale)			700			1006						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		61		803		05		Reproduction (youngfemale)			1010			1363						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		122		810		05		Reproduction (youngfemale)			870			1164						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		255		633		05		Reproduction (youngfemale)			260			1758						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		121		636		05		Reproduction (youngfemale)			390			1101			1182		1182			Ceriodaphnia		Invert		Author reported
	Amphipod Hyalella azteca		95		61		051		Biomass (28 d)			1993			1014						Hyalella			Author reported
	Amphipod Hyalella azteca		106		604		033		Biomass (28 d)			425			2895			1713		1713			Hyalella		Invert		Author reported
	Midge Chironomus riparius		118		558		18		Adult midge emergence			2955			1192						Chironomus			Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Midge Chironomus riparius		119		505		18		Adult midge emergence			8442			22578						Chironomus			Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Midge Chironomus riparius		91		66		051		Reproduction ( of eggscase)			3387			7664			5908		5908			Chironomus		Invert		Author reported
	Atlantic salmon Salmo salar		127		57		18		Biomass			6156			5085			5085		5085			Salmo		Fish		Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Brook trout Salvelinus fontinalis		123		655		19		Biomass			1644			270						Salvelinus			Author reported		wellRO mix
	Brook trout Salvelinus fontinalis		128		565		18		Biomass			1435			1295			5914		5914			Salvelinus		Fish		Author reported		wellRO mix
	Fathead minnow Pimephales promelas		220		770		16		Biomass			6194			3569						Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		well water
	Fathead minnow Pimephales promelas		96		620		025		Fry survival			4286			1734			2488		2488			Pimephales		Fish		Author reported half the detection limit
	Zebrafish Danio rerio		83		61		025		Biomass			2344			1102			1102		1102			Danio		Fish		Author reported half the detection limit
	Wood frog Rana sylvatica		115		469		16		MATC-survival		gt		2000		gt		9746		gt		9746		9746		gt		Rana		Amphib - Other Data		Value is from 2007 FW Cu AWQC Appendix C recommendation		aged tap water

	Appendix A Acceptable Acute Toxicity Data of Aluminum to Freshwater Aquatic Animals
	Species		Methoda		Hardness (mgL as CaCO3)		pH		DOC(mgL)			LC50 or EC50 Conc (microgL)		Reference			Normalized Conc - LC50(microgL)			Species Mean Acute Value(microgL)		Genus Mean Acute Value(microgL)			Genus			DOC Note		Dilution water
	Snail Physa sp		S M T		474		659		11		gt		23400		Call 1984 Call et al 1984		gt		48425			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		755		11			30600		Call 1984 Call et al 1984			26986			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		817		11		gt		24700		Call 1984 Call et al 1984		gt		24040			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		746		11			55500		Call 1984 Call et al 1984			50338			35462		35462			Physa		Mollusk		Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Fatmucket Lampsilis siliquoidea		F M T		1060		612		048		gt		6302		Wang et al 2016 Wang et al 2017		gt		29834		gt		29834		29834		gt		Lampsilis		Mollusk		Author reported
	Cladoceran Ceriodaphnia dubia		S M A		500		742		11			1900		McCauley et al 1986			1728			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		S M A		505		786		11			1500		McCauley et al 1986			1294			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		UW-S Lake Superior lab water
	Cladoceran Ceriodaphnia dubia		S M A		500		813		11			2560		McCauley et al 1986			2431			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		R M T		26		75		05			720		ENSR 1992d			1113			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		R M T		46		76		05			1880		ENSR 1992d			2495			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		R M T		96		78		05			2450		ENSR 1992d			2894			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		S U NR		90		715		05			3727		Fort and Stover 1995			5002			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U NR		90		715		05			5673		Fort and Stover 1995			7613			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U NR		89		76		05			2880		Soucek et al 2001			3362			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		601		05			7112		European Al Association 2009			1691			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		605		2			6865		European Al Association 2009			7088			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		609		4			15581		European Al Association 2009			10072			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		601		05			681		European Al Association 2009			1620			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		603		05			1630		European Al Association 2009			3674			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		597		05			1785		European Al Association 2009			4732			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		592		05			1410		European Al Association 2009			4295			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		699		05		gt		1300		European Al Association 2009		gt		4189			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		785		05		gt		5000		European Al Association 2009		gt		7960			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		68		2		gt		10000		European Al Association 2009		gt		20755			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		782		2		gt		15000		European Al Association 2009		gt		11626			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		677		4		gt		10000		European Al Association 2009		gt		15161			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		766		4		gt		15000		European Al Association 2009		gt		8608			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		79		05		gt		2000		European Al Association 2009		gt		3150			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		789		05		gt		2000		European Al Association 2009		gt		3156			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		604		05			1108		European Al Association 2009			850			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		598		2			11371		European Al Association 2009			4817			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		573		4			80467		European Al Association 2009			43570			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		671		05		gt		10000		European Al Association 2009		gt		23786			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		783		05		gt		5000		European Al Association 2009		gt		6344			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		679		2		gt		10000		European Al Association 2009		gt		10394			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		767		2		gt		15000		European Al Association 2009		gt		9082			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		668		4		gt		15000		European Al Association 2009		gt		12461			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		762		4		gt		15000		European Al Association 2009		gt		6332			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		606		2			33868		European Al Association 2009			7920			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		56		4			104842		European Al Association 2009			48800			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		693		05		gt		5000		European Al Association 2009		gt		7126			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		120		788		05		gt		5000		European Al Association 2009		gt		5898			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		120		676		2		gt		15000		European Al Association 2009		gt		12117			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		771		2		gt		15000		European Al Association 2009		gt		8096			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		66		4		gt		15000		European Al Association 2009		gt		10139			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		76		4		gt		15000		European Al Association 2009		gt		5545			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			11971		European Al Association 2010			2699			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			27478		European Al Association 2010			6194			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			11998		European Al Association 2010			2705			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		607		05			92495		European Al Association 2010			1876			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		609		05			31337		European Al Association 2010			6035			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		61		05			33235		European Al Association 2010			6238			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		708		05		gt		8864		European Al Association 2010		gt		2534			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		779		05		gt		42783		European Al Association 2010		gt		6930			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		753		05			13204		European Al Association 2010			244			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		600		601		05			46326		European Al Association 2010			3798			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		600		599		05		gt		8590		European Al Association 2010		gt		7366			5094				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia reticulata		F M T		451		60		11			304		Shephard 1983			2009			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia reticulata		F M T		40		55		11			362		Shephard 1983			55307			10542		7328			Ceriodaphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake SuperiorDI Mix
	Cladoceran Daphnia magna		S U NR		453		774		11			3900		Biesinger and Christensen 1972			3382			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Daphnia magna		S M T		220		76		16			38200		Kimball 1978			20303			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Well water
	Cladoceran Daphnia magna		S U T		451		725		11			2800		Shephard 1983			2868			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Daphnia magna		S U T		168		599		05			5000		European Al Association 2009		gt		2265			-				Daphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Daphnia magna		S U T		168		792		05			7950		European Al Association 2009			920			3332		3332			Daphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Ostracod Stenocypris major		R M T		1563		651		16			310196		Shuhaimi-Othman et al 2011a			10216			10216		10216			Stenocypris		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (Malaysia)
	Amphipod Crangonyx pseudogracilis		R U T		50		675		16			9190		Martin and Holdich 1986			12174			12174		12174			Crangonyx		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (UK)
	Amphipod Hyalella azteca		F M T		105		613		048		gt		5997		Wang et al 2016 Wang et al 2017		gt		28002		gt		28002		28002		gt		Hyalella		Invert		Author reported
	Stonefly Acroneuria sp		S M T		474		746		11		gt		22600		Call et al 1984		gt		20498		gt		20498		20498		gt		Acroneuria		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Midge Chironomus plumosus		S U T		80		70		16			30000		Fargasova 2001			25361			25361		25361			Chironomus		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (Slovakia)
	Midge Paratanytarsus dissimilis		S M T		1743		728		28		gt		77700		Lamb and Bailey 1981 1983		gt		62318		gt		62318		62318		gt		Paratanytarsus		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Liberty Lake Washington
	Rainbow trout Oncorhynchus mykiss		F M T		274		758		05		gt		9840		Gundersen et al 1994		gt		5745			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		452		762		05		gt		8070		Gundersen et al 1994		gt		5212			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		895		758		05		gt		8160		Gundersen et al 1994		gt		6807			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		1304		758		05		gt		8200		Gundersen et al 1994		gt		7662			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		232		825		05			6170		Gundersen et al 1994			1190			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		350		826		05			6170		Gundersen et al 1994			1523			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		836		829		05			7670		Gundersen et al 1994			3205			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		1158		830		05			6930		Gundersen et al 1994			3554			3661		3661			Oncorhynchus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Atlantic salmon Salmo salar		S U T		68		55		05			584		Hamilton and Haines 1995			21042			-				Salmo			Value is from 2007 FW Cu AWQC Appendix C recommendation		Soft recon
	Atlantic salmon Salmo salar		S U T		68		65		05			599		Hamilton and Haines 1995			2430			7151		7151			Salmo		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Soft recon
	Brook trout Salvelinus fontinalis		S U T		40		56		16			6530		Tandjung 1982			31767			-				Salvelinus			Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Brook trout Salvelinus fontinalis		S U T		18		56		16			3400		Tandjung 1982			28391			-				Salvelinus			Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Brook trout Salvelinus fontinalis		S U T		2		56		16			370		Tandjung 1982			13663			23097		23097			Salvelinus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Green sunfish Lepomis cyanellus		S M T		474		755		11		gt		50000		Call et al 1984		gt		24028		gt		24028		24028		gt		Lepomis		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Rio Grande silvery minnow Hybognathus amarus		R M T		140		81		05		gt		59100		Buhl 2002		gt		39414		gt		39414		39414		gt		Hybognathus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon water
	Fathead minnow Pimephales promelas		S M T		474		761		11		gt		48200		Call et al 1984		gt		21522			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Fathead minnow Pimephales promelas		S M T		474		805		11		gt		49800		Call et al 1984		gt		12972			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Fathead minnow Pimephales promelas		R M T		26		78		05			1160		ENSR 1992c			477			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		46		76		05			8180		ENSR 1992c			5446			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		96		81		05			20300		ENSR 1992c			10966			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		194		81		05			44800		ENSR 1992c			35847			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		140		81		05		gt		59100		Buhl 2002		gt		39414			10168		10168			Pimephales		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon water
	Smallmouth bass Micropterus dolomieui		S M T		1245		505		16			130		Kane and Rabeni 1987			3929			3929		3929			Micropterus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Green tree frog Hyla cinerea		R M T		455		549		05		gt		4052		Jung and Jagoe 1995		gt		20016		gt		20016		20016		gt		Hyla		Amphibian		Value is from 2007 FW Cu AWQC Appendix C recommendation		artificial soft recon

	Normalization Chemistry
	pH		7					FAV		2741
	Hardness		100					CMC		1400
	DOC		1					CCC		390
	Acute Ranked GMAV											Chronic Ranked GMCV
	Rank			GMAV		Genus								Rank			GMCV		Genus
	18		gt		62318		Paratanytarsus		Invert							12			17098		Aeolosoma		Invert
	17		gt		39414		Hybognathus		Fish							11		gt		9746		Rana		Amphib - Other Data
	16			35462		Physa		Mollusk							10			5908		Chironomus		Invert
	15		gt		29834		Lampsilis		Mollusk							9			4877		Lymnaea		Mollusk
	14		gt		28002		Hyalella		Invert							8			2555		Brachionus		Invert
	13			25361		Chironomus		Invert							7			2488		Pimephales		Fish
	12		gt		24028		Lepomis		Fish							6			1713		Hyalella		Invert
	11			23097		Salvelinus		Fish							5			1182		Ceriodaphnia		Invert
	10		gt		20498		Acroneuria		Invert							4			1102		Danio		Fish
	9		gt		20016		Hyla		Amphibian							3			1042		Lampsilis		Mollusk
	8			12174		Crangonyx		Invert							2			591		Salvelinus		Fish
	7			10216		Stenocypris		Invert							1			509		Salmo		Fish
	6			10168		Pimephales		Fish
	5			7328		Ceriodaphnia		Invert
	4			7151		Salmo		Fish
	3			3929		Micropterus		Fish
	2			3661		Oncorhynchus		Fish
	1			3332		Daphnia		Invert
		N		Rank		Genus		GMAV		ln(GMAV)		ln(GMAV)2		P=R(N+1)		sqrt(P)				N		Rank		Genus		GMCV		ln(GMCV)		ln(GMCV)2		P=R(N+1)		sqrt(P)
		18		4		Salmo		7151		887		7876		0211		0459				12		4		Danio		1102		700		4907		0308		0555
			3		Micropterus		3929		828		6849		0158		0397					3		Lampsilis		1042		695		4829		0231		0480
			2		Oncorhynchus		3661		821		6733		0105		0324					2		Salvelinus		591		638		4074		0154		0392
			1		Daphnia		3332		811		6579		0053		0229					1		Salmo		509		623		3883		0077		0277
			Sum				3347		2804		0526		141					Sum				2657		1769		0769		170
			S2 =		1223										S2 =		1080
			L =		7134										L =		5242
			A =		7916										A =		5976
			FAV =		2741										FCV =		3940
			CMC =		1371

				All concentrations reported are microgL total Aluminum
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	ENTER DATA HERE									ERRORNUM		ACUTE														CHRONIC
										Rank 1				Rank 2				Rank 3				Rank 4					Rank 1				Rank 2				Rank 3				Rank 4
	SiteName		DOC(mgL)		Hardness(mgL as CaCO3)		pH			FAV		CMC		CCC			Genus		GMAV			Genus		GMAV			Genus		GMAV			Genus		GMAV				Genus		GMCV			Genus		GMCV			Genus		GMCV			Genus		GMCV
	Site 1		50		25		9		75428737034162		35229324666008		1800		770			Daphnia			33452		Ceriodaphnia			73576		Stenocypris			102576		Crangonyx			122230			Lampsilis			10463		Ceriodaphnia			11865		Hyalella			17200		Brachionus			25648
	Site 2		50		50		9		75428737034162		31204991509063		1600		680			Daphnia			29631		Ceriodaphnia			65171		Stenocypris			90859		Crangonyx			108267			Lampsilis			9268		Ceriodaphnia			10510		Hyalella			15236		Brachionus			22718
	Site 3		50		75		9		75428737034162		29067527928199		1500		640			Daphnia			27601		Ceriodaphnia			60707		Stenocypris			84635		Crangonyx			100851			Lampsilis			8633		Ceriodaphnia			9790		Hyalella			14192		Brachionus			21162
	Site 4		50		100		9		75428737034162		27640367912592		1400		600			Daphnia			26246		Ceriodaphnia			57726		Stenocypris			80480		Crangonyx			95899			Lampsilis			8209		Ceriodaphnia			9309		Hyalella			13495		Brachionus			20123
	Site 5		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 6		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 7		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 8		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 9		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 10		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 11		50		25		85		75428737034162		63245275664384		3200		1400			Daphnia			60055		Ceriodaphnia			132086		Stenocypris			184149		Crangonyx			219432			Lampsilis			18783		Ceriodaphnia			21301		Hyalella			30879		Brachionus			46045
	Site 12		50		50		85		75428737034162		61388044609926		3100		1300			Daphnia			58291		Ceriodaphnia			128208		Stenocypris			178742		Crangonyx			212988			Lampsilis			18232		Ceriodaphnia			20676		Hyalella			29972		Brachionus			44693
	Site 13		50		75		85		75428737034162		60327020429132		3000		1300			Daphnia			57284		Ceriodaphnia			125992		Stenocypris			175652		Crangonyx			209307			Lampsilis			17917		Ceriodaphnia			20318		Hyalella			29454		Brachionus			43920
	Site 14		50		100		85		75428737034162		59585352130146		3000		1300			Daphnia			56580		Ceriodaphnia			124443		Stenocypris			173493		Crangonyx			206734			Lampsilis			17696		Ceriodaphnia			20068		Hyalella			29092		Salmo			41264
	Site 15		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 16		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 17		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 18		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 19		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 20		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370

IV Construct sensitivity distribution to determine criteria at set conditions

21

Figure 8 Ranked Summary of Total Aluminum Genus Mean Acute Values (GMAVs) - Freshwater at pH 7 Hardness of 100 mgL and DOC of 1 mgL

IV Construct sensitivity distribution to determine criteria at set conditions

22

Figure 10 Ranked Summary of Total Aluminum Genus Mean Chronic Values (GMCVs) ndash Freshwater Supplemented with Other Data to Fulfill Missing MDRs at pH 7 Hardness of 100 mgL and DOC of 1 mgL

Aluminum Criteria Calculatorbull The relative ranking of genera in the sensitivity

distribution to which the toxicity data are normalized will change based on the set of water chemistry conditions (ie pH hardness and DOC)

bull To make calculating aluminum criteria easier EPA developed an Aluminum Criteria Calculatorndash Automated so that user only needs to enter water chemistry

conditions of interestndash Allows quick calculations for a variety of conditionsndash Limits calculations to the recommended conditions for which

the MLR models were developed were specified for hardness and DOC

bull No extrapolation beyond hardness and DOC empirical data bounds

23

Aluminum Criteria Calculator SD Example

24

pH=7 Hardness=100 DOC=1 pH=6 Hardness=25 DOC=1

Chronic Ranked GMCVRank GMCV Genus

12 17098 Aeolosoma Invert11 gt 9746 Rana Amphib - Other Data10 5908 Chironomus Invert

9 4877 Lymnaea Mollusk8 2555 Brachionus Invert7 2488 Pimephales Fish6 1713 Hyalella Invert5 1182 Ceriodaphnia Invert4 1102 Danio Fish3 1042 Lampsilis Mollusk2 591 Salvelinus Fish1 509 Salmo Fish

FCV = 3940 microgL total aluminum

Chronic Ranked GMCVRank GMCV Genus

12 gt 2131 Rana Amphib - Other Data11 1710 Aeolosoma Invert10 591 Chironomus Invert

9 544 Pimephales Fish8 488 Lymnaea Mollusk7 255 Brachionus Invert6 241 Danio Fish5 171 Hyalella Invert4 129 Salvelinus Fish3 118 Ceriodaphnia Invert2 111 Salmo Fish1 104 Lampsilis Mollusk

FCV = 987 microgL total aluminum

Multiple Scenarios

Run Scenarios

Summary Sheet

Acute Dataset

Chronic Dataset

Multiple Scenarios

Run Scenarios

Summary Sheet

Acute Dataset

Chronic Dataset

Boundaries of the Criteria Outputsbull MLR models were developed using data that

encompassndash DOC range of 008-5 mgLndash Hardness range of 98-127 mgL (as CaCO3)ndash pH range of 6-81

bull MLR criteria outputs are bounded atndash Maximum of 150 mgL total hardness (as CaCO3)

ndash DOC of 50 mgLndash pH range of the model is 50 to 90

25

Aluminum Criteria Look-Up Tables

26

bull EPA also provided criteria look-up tables in Appendix K

Har

dnes

s

CCC(microgL total aluminum)

(DOC=05 mgL)

pH 50 pH 55 pH 60 pH 65 pH 70 pH 75 pH 80 pH 85 pH 90

25 23 a 14 b 69 c 160 f 350 f 620 i 540 b 410 a 230 a

50 43 a 24 b 100 d 170 f 310 f 630 f 670 j 400 a 200 a

75 61 a 33 b 130 e 180 f 290 f 530 f 730 i 390 a 190 a

100 78 a 41 b 150 e 190 f 280 g 460 f 770 c 390 a 180 a

150 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

200 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

250 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

300 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

350 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

400 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

Aluminum Criteria Look-Up Tables

27

bull EPA also provided criteria look-up tables in Appendix K

Har

dnes

s

CCC(microgL total aluminum)

(DOC=10 mgL)

pH 50 pH 55 pH 60 pH 65 pH 70 pH 75 pH 80 pH 85 pH 90

25 33 a 21 b 99 c 220 f 490 f 900 i 780 b 590 a 330 a

50 61 a 35 b 150 e 250 f 440 f 890 f 960 j 580 a 290 a

75 88 a 47 b 180 e 260 f 410 f 740 f 1100 i 570 a 270 a

100 11 a 59 b 210 e 270 f 390 g 650 f 1100 c 560 b 260 a

150 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

200 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

250 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

300 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

350 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

400 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

Aluminum Criteria Look-Up Tables

28

bull EPA also provided criteria look-up tables in Appendix K

Har

dnes

s

CCC(microgL total aluminum)

(DOC=25 mgL)

pH 50 pH 55 pH 60 pH 65 pH 70 pH 75 pH 80 pH 85 pH 90

25 54 a 34 b 160 c 350 f 770 f 1500 i 1300 b 960 a 540 a

50 99 a 57 b 230 e 390 f 690 f 1400 f 1600 j 930 a 470 a

75 14 a 77 b 290 e 410 f 640 f 1200 f 1700 i 920 a 440 a

100 18 a 96 b 330 e 420 f 620 g 1000 f 1800 d 920 b 420 a

150 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

200 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

250 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

300 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

350 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

400 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

Aluminum Criteria Look-Up Tables

29

bull EPA also provided criteria look-up tables in Appendix K

Har

dnes

s

CCC(microgL total aluminum)

(DOC=50 mgL)

pH 50 pH 55 pH 60 pH 65 pH 70 pH 75 pH 80 pH 85 pH 90

25 77 a 49 b 230 c 500 f 1100 f 2100 c 1900 b 1400 a 770 a

50 14 a 82 b 330 e 540 f 970 f 2000 f 2200 j 1300 a 680 a

75 20 a 110 b 410 e 570 f 900 f 1600 f 2500 i 1300 a 640 a

100 26 a 140 b 460 f 590 f 880 g 1400 f 2500 d 1300 b 600 a

150 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

200 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

250 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

300 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

350 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

400 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

Status

30

bull Draft Document Released to the Federal Register on 72817ndash Public comment period of 60 days ndash Due Date for Comments is 92617

bull Revisions will be made considering public commentsbull Subsequent EPA intra-agency review of final criteria

document will then be conducted bull Publication of final criteria document planned for Late Fall

2017

Federal Register Webpagehttpswwwfederalregistergovdocuments201

707282017-15968request-for-scientific-views-draft-updated-aquatic-life-ambient-water-quality-criteria-for-aluminum

Aluminum Criteria Webpagehttpswwwepagovwqc2017-draft-aquatic-life-

criteria-aluminum-freshwater-documents

31

	Appendix C Acceptable Chronic Toxicity Data of Aluminum to Freshwater Aquatic Animals
									MLR equation
	Species		Hardness (mgL as CaCO3)		pH		DOC(mgL)		EC20 Endpoint			EC20(microgL)			Normalized Conc- EC20(microgL)			SMCV-EC20(microgL)		GMCV-EC20(microgL)			Genus			DOC Notes		Dilution water
	Oligochaete Aeolosoma sp		48		6		025		Reproduction (population size)			1235			1710			1710		1710			Aeolosoma		Invert		Author reported half the detection limit
	Rotifer Brachionus calyciflorus		100		62		025		Reproduction (population size)			431			255			255		255			Brachionus		Invert		Author reported half the detection limit
	Great pond snail Lymnaea stagnalis		117		61		025		Biomass			746			488			488		488			Lymnaea		Mollusk		Author reported half the detection limit
	Fatmucket Lampsilis siliquoidea		1055		604		040		Biomass			169			104			104		104			Lampsilis		Mollusk		Author reported
	Cladoceran Ceriodaphnia dubia		50		715		11		Reproduction - youngstarting adult			1780			190						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		26		73		05		Reproduction - youngfemale			1557			272						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		46		75		05		Reproduction - youngfemale			8087			110						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		96		79		05		Reproduction - youngfemale			6519			80						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		194		81		05		Reproduction - youngfemale			6836			89						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		255		703		05		Reproduction (youngfemale)			250			56						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		122		713		05		Reproduction (youngfemale)			860			106						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		25		798		05		Reproduction (youngfemale)			700			101						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		61		803		05		Reproduction (youngfemale)			1010			136						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		122		810		05		Reproduction (youngfemale)			870			116						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		255		633		05		Reproduction (youngfemale)			260			176						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		121		636		05		Reproduction (youngfemale)			390			110			118		118			Ceriodaphnia		Invert		Author reported
	Amphipod Hyalella azteca		95		61		051		Biomass (28 d)			1993			101						Hyalella			Author reported
	Amphipod Hyalella azteca		106		604		033		Biomass (28 d)			425			289			171		171			Hyalella		Invert		Author reported
	Midge Chironomus riparius		118		558		18		Adult midge emergence			2955			119						Chironomus			Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Midge Chironomus riparius		119		505		18		Adult midge emergence			8442			2258						Chironomus			Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Midge Chironomus riparius		91		66		051		Reproduction ( of eggscase)			3387			766			591		591			Chironomus		Invert		Author reported
	Atlantic salmon Salmo salar		127		57		18		Biomass			6156			1112			1112		1112			Salmo		Fish		Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Brook trout Salvelinus fontinalis		123		655		19		Biomass			1644			59						Salvelinus			Author reported		wellRO mix
	Brook trout Salvelinus fontinalis		128		565		18		Biomass			1435			283			1293		1293			Salvelinus		Fish		Author reported		wellRO mix
	Fathead minnow Pimephales promelas		220		770		16		Biomass			6194			781						Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		well water
	Fathead minnow Pimephales promelas		96		620		025		Fry survival			4286			379			544		544			Pimephales		Fish		Author reported half the detection limit
	Zebrafish Danio rerio		83		61		025		Biomass			2344			241			241		241			Danio		Fish		Author reported half the detection limit
	Wood frog Rana sylvatica		115		469		16		MATC-survival		gt		2000		gt		2131		gt		2131		2131		gt		Rana		Amphib - Other Data		Value is from 2007 FW Cu AWQC Appendix C recommendation		aged tap water

	Appendix A Acceptable Acute Toxicity Data of Aluminum to Freshwater Aquatic Animals
	Species		Methoda		Hardness (mgL as CaCO3)		pH		DOC(mgL)			LC50 or EC50 Conc (microgL)		Reference			Normalized Conc - LC50(microgL)			Species Mean Acute Value(microgL)		Genus Mean Acute Value(microgL)			Genus			DOC Note		Dilution water
	Snail Physa sp		S M T		474		659		11		gt		23400		Call 1984 Call et al 1984		gt		4843			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		755		11			30600		Call 1984 Call et al 1984			2699			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		817		11		gt		24700		Call 1984 Call et al 1984		gt		2404			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		746		11			55500		Call 1984 Call et al 1984			5034			3546		3546			Physa		Mollusk		Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Fatmucket Lampsilis siliquoidea		F M T		1060		612		048		gt		6302		Wang et al 2016 Wang et al 2017		gt		2983		gt		2983		2983		gt		Lampsilis		Mollusk		Author reported
	Cladoceran Ceriodaphnia dubia		S M A		500		742		11			1900		McCauley et al 1986			173			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		S M A		505		786		11			1500		McCauley et al 1986			129			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		UW-S Lake Superior lab water
	Cladoceran Ceriodaphnia dubia		S M A		500		813		11			2560		McCauley et al 1986			243			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		R M T		26		75		05			720		ENSR 1992d			111			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		R M T		46		76		05			1880		ENSR 1992d			250			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		R M T		96		78		05			2450		ENSR 1992d			289			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		S U NR		90		715		05			3727		Fort and Stover 1995			500			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U NR		90		715		05			5673		Fort and Stover 1995			761			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U NR		89		76		05			2880		Soucek et al 2001			336			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		601		05			7112		European Al Association 2009			169			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		605		2			6865		European Al Association 2009			709			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		609		4			15581		European Al Association 2009			1007			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		601		05			681		European Al Association 2009			162			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		603		05			1630		European Al Association 2009			367			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		597		05			1785		European Al Association 2009			473			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		592		05			1410		European Al Association 2009			429			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		699		05		gt		1300		European Al Association 2009		gt		419			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		785		05		gt		5000		European Al Association 2009		gt		796			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		68		2		gt		10000		European Al Association 2009		gt		2076			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		782		2		gt		15000		European Al Association 2009		gt		1163			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		677		4		gt		10000		European Al Association 2009		gt		1516			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		766		4		gt		15000		European Al Association 2009		gt		861			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		79		05		gt		2000		European Al Association 2009		gt		315			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		789		05		gt		2000		European Al Association 2009		gt		316			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		604		05			1108		European Al Association 2009			85			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		598		2			11371		European Al Association 2009			482			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		573		4			80467		European Al Association 2009			4357			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		671		05		gt		10000		European Al Association 2009		gt		2379			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		783		05		gt		5000		European Al Association 2009		gt		634			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		679		2		gt		10000		European Al Association 2009		gt		1039			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		767		2		gt		15000		European Al Association 2009		gt		908			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		668		4		gt		15000		European Al Association 2009		gt		1246			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		762		4		gt		15000		European Al Association 2009		gt		633			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		606		2			33868		European Al Association 2009			792			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		56		4			104842		European Al Association 2009			4880			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		693		05		gt		5000		European Al Association 2009		gt		713			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		120		788		05		gt		5000		European Al Association 2009		gt		590			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		120		676		2		gt		15000		European Al Association 2009		gt		1212			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		771		2		gt		15000		European Al Association 2009		gt		810			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		66		4		gt		15000		European Al Association 2009		gt		1014			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		76		4		gt		15000		European Al Association 2009		gt		555			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			11971		European Al Association 2010			270			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			27478		European Al Association 2010			619			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			11998		European Al Association 2010			270			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		607		05			92495		European Al Association 2010			188			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		609		05			31337		European Al Association 2010			604			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		61		05			33235		European Al Association 2010			624			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		708		05		gt		8864		European Al Association 2010		gt		253			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		779		05		gt		42783		European Al Association 2010		gt		693			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		753		05			13204		European Al Association 2010			24			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		600		601		05			46326		European Al Association 2010			380			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		600		599		05		gt		8590		European Al Association 2010		gt		737			509				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia reticulata		F M T		451		60		11			304		Shephard 1983			201			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia reticulata		F M T		40		55		11			362		Shephard 1983			5531			1054		733			Ceriodaphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake SuperiorDI Mix
	Cladoceran Daphnia magna		S U NR		453		774		11			3900		Biesinger and Christensen 1972			338			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Daphnia magna		S M T		220		76		16			38200		Kimball 1978			2030			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Well water
	Cladoceran Daphnia magna		S U T		451		725		11			2800		Shephard 1983			287			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Daphnia magna		S U T		168		599		05			5000		European Al Association 2009		gt		227			-				Daphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Daphnia magna		S U T		168		792		05			7950		European Al Association 2009			92			333		333			Daphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Ostracod Stenocypris major		R M T		1563		651		16			310196		Shuhaimi-Othman et al 2011a			1022			1022		1022			Stenocypris		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (Malaysia)
	Amphipod Crangonyx pseudogracilis		R U T		50		675		16			9190		Martin and Holdich 1986			1217			1217		1217			Crangonyx		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (UK)
	Amphipod Hyalella azteca		F M T		105		613		048		gt		5997		Wang et al 2016 Wang et al 2017		gt		2800		gt		2800		2800		gt		Hyalella		Invert		Author reported
	Stonefly Acroneuria sp		S M T		474		746		11		gt		22600		Call et al 1984		gt		2050		gt		2050		2050		gt		Acroneuria		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Midge Chironomus plumosus		S U T		80		70		16			30000		Fargasova 2001			2536			2536		2536			Chironomus		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (Slovakia)
	Midge Paratanytarsus dissimilis		S M T		1743		728		28		gt		77700		Lamb and Bailey 1981 1983		gt		6232		gt		6232		6232		gt		Paratanytarsus		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Liberty Lake Washington
	Rainbow trout Oncorhynchus mykiss		F M T		274		758		05		gt		9840		Gundersen et al 1994		gt		1256			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		452		762		05		gt		8070		Gundersen et al 1994		gt		1140			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		895		758		05		gt		8160		Gundersen et al 1994		gt		1489			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		1304		758		05		gt		8200		Gundersen et al 1994		gt		1676			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		232		825		05			6170		Gundersen et al 1994			260			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		350		826		05			6170		Gundersen et al 1994			333			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		836		829		05			7670		Gundersen et al 1994			701			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		1158		830		05			6930		Gundersen et al 1994			777			801		801			Oncorhynchus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Atlantic salmon Salmo salar		S U T		68		55		05			584		Hamilton and Haines 1995			4602			-				Salmo			Value is from 2007 FW Cu AWQC Appendix C recommendation		Soft recon
	Atlantic salmon Salmo salar		S U T		68		65		05			599		Hamilton and Haines 1995			531			1564		1564			Salmo		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Soft recon
	Brook trout Salvelinus fontinalis		S U T		40		56		16			6530		Tandjung 1982			6947			-				Salvelinus			Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Brook trout Salvelinus fontinalis		S U T		18		56		16			3400		Tandjung 1982			6209			-				Salvelinus			Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Brook trout Salvelinus fontinalis		S U T		2		56		16			370		Tandjung 1982			2988			5051		5051			Salvelinus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Green sunfish Lepomis cyanellus		S M T		474		755		11		gt		50000		Call et al 1984		gt		5255		gt		5255		5255		gt		Lepomis		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Rio Grande silvery minnow Hybognathus amarus		R M T		140		81		05		gt		59100		Buhl 2002		gt		8620		gt		8620		8620		gt		Hybognathus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon water
	Fathead minnow Pimephales promelas		S M T		474		761		11		gt		48200		Call et al 1984		gt		4707			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Fathead minnow Pimephales promelas		S M T		474		805		11		gt		49800		Call et al 1984		gt		2837			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Fathead minnow Pimephales promelas		R M T		26		78		05			1160		ENSR 1992c			104			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		46		76		05			8180		ENSR 1992c			1191			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		96		81		05			20300		ENSR 1992c			2398			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		194		81		05			44800		ENSR 1992c			7839			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		140		81		05		gt		59100		Buhl 2002		gt		8620			2224		2224			Pimephales		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon water
	Smallmouth bass Micropterus dolomieui		S M T		1245		505		16			130		Kane and Rabeni 1987			859			859		859			Micropterus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Green tree frog Hyla cinerea		R M T		455		549		05		gt		4052		Jung and Jagoe 1995		gt		4377		gt		4377		4377		gt		Hyla		Amphibian		Value is from 2007 FW Cu AWQC Appendix C recommendation		artificial soft recon

	Normalization Chemistry
	pH		6					FAV		360
	Hardness		25					CMC		180
	DOC		1					CCC		99
	Acute Ranked GMAV											Chronic Ranked GMCV
	Rank			GMAV		Genus								Rank			GMCV		Genus
	18		gt		8620		Hybognathus		Fish							12		gt		2131		Rana		Amphib - Other Data
	17		gt		6232		Paratanytarsus		Invert							11			1710		Aeolosoma		Invert
	16		gt		5255		Lepomis		Fish							10			591		Chironomus		Invert
	15			5051		Salvelinus		Fish							9			544		Pimephales		Fish
	14		gt		4377		Hyla		Amphibian							8			488		Lymnaea		Mollusk
	13			3546		Physa		Mollusk							7			255		Brachionus		Invert
	12		gt		2983		Lampsilis		Mollusk							6			241		Danio		Fish
	11		gt		2800		Hyalella		Invert							5			171		Hyalella		Invert
	10			2536		Chironomus		Invert							4			129		Salvelinus		Fish
	9			2224		Pimephales		Fish							3			118		Ceriodaphnia		Invert
	8		gt		2050		Acroneuria		Invert							2			111		Salmo		Fish
	7			1564		Salmo		Fish							1			104		Lampsilis		Mollusk
	6			1217		Crangonyx		Invert
	5			1022		Stenocypris		Invert
	4			859		Micropterus		Fish
	3			801		Oncorhynchus		Fish
	2			733		Ceriodaphnia		Invert
	1			333		Daphnia		Invert
		N		Rank		Genus		GMAV		ln(GMAV)		ln(GMAV)2		P=R(N+1)		sqrt(P)				N		Rank		Genus		GMCV		ln(GMCV)		ln(GMCV)2		P=R(N+1)		sqrt(P)
		18		4		Micropterus		859		676		4564		0211		0459				12		4		Salvelinus		129		486		2364		0308		0555
			3		Oncorhynchus		801		669		4469		0158		0397					3		Ceriodaphnia		118		477		2277		0231		0480
			2		Ceriodaphnia		733		660		4352		0105		0324					2		Salmo		111		471		2220		0154		0392
			1		Daphnia		333		581		3374		0053		0229					1		Lampsilis		104		465		2159		0077		0277
			Sum				2585		1676		0526		141					Sum				1899		902		0769		170
			S2 =		1987										S2 =		059
			L =		4890										L =		4420
			A =		5887										A =		4592
			FAV =		360										FCV =		987
			CMC =		180

				All concentrations reported are microgL total Aluminum
				lt-----Click Button after entering data
	ENTER DATA HERE									ERRORNUM		ACUTE														CHRONIC
										Rank 1				Rank 2				Rank 3				Rank 4					Rank 1				Rank 2				Rank 3				Rank 4
	SiteName		DOC(mgL)		Hardness(mgL as CaCO3)		pH			FAV		CMC		CCC			Genus		GMAV			Genus		GMAV			Genus		GMAV			Genus		GMAV				Genus		GMCV			Genus		GMCV			Genus		GMCV			Genus		GMCV
	Site 1		50		25		9		75428737034162		35229324666008		1800		770			Daphnia			33452		Ceriodaphnia			73576		Stenocypris			102576		Crangonyx			122230			Lampsilis			10463		Ceriodaphnia			11865		Hyalella			17200		Brachionus			25648
	Site 2		50		50		9		75428737034162		31204991509063		1600		680			Daphnia			29631		Ceriodaphnia			65171		Stenocypris			90859		Crangonyx			108267			Lampsilis			9268		Ceriodaphnia			10510		Hyalella			15236		Brachionus			22718
	Site 3		50		75		9		75428737034162		29067527928199		1500		640			Daphnia			27601		Ceriodaphnia			60707		Stenocypris			84635		Crangonyx			100851			Lampsilis			8633		Ceriodaphnia			9790		Hyalella			14192		Brachionus			21162
	Site 4		50		100		9		75428737034162		27640367912592		1400		600			Daphnia			26246		Ceriodaphnia			57726		Stenocypris			80480		Crangonyx			95899			Lampsilis			8209		Ceriodaphnia			9309		Hyalella			13495		Brachionus			20123
	Site 5		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 6		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 7		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 8		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 9		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 10		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 11		50		25		85		75428737034162		63245275664384		3200		1400			Daphnia			60055		Ceriodaphnia			132086		Stenocypris			184149		Crangonyx			219432			Lampsilis			18783		Ceriodaphnia			21301		Hyalella			30879		Brachionus			46045
	Site 12		50		50		85		75428737034162		61388044609926		3100		1300			Daphnia			58291		Ceriodaphnia			128208		Stenocypris			178742		Crangonyx			212988			Lampsilis			18232		Ceriodaphnia			20676		Hyalella			29972		Brachionus			44693
	Site 13		50		75		85		75428737034162		60327020429132		3000		1300			Daphnia			57284		Ceriodaphnia			125992		Stenocypris			175652		Crangonyx			209307			Lampsilis			17917		Ceriodaphnia			20318		Hyalella			29454		Brachionus			43920
	Site 14		50		100		85		75428737034162		59585352130146		3000		1300			Daphnia			56580		Ceriodaphnia			124443		Stenocypris			173493		Crangonyx			206734			Lampsilis			17696		Ceriodaphnia			20068		Hyalella			29092		Salmo			41264
	Site 15		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 16		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 17		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 18		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 19		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 20		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370

	Appendix C Acceptable Chronic Toxicity Data of Aluminum to Freshwater Aquatic Animals
									MLR equation
	Species		Hardness (mgL as CaCO3)		pH		DOC(mgL)		EC20 Endpoint			EC20(microgL)			Normalized Conc- EC20(microgL)			SMCV-EC20(microgL)		GMCV-EC20(microgL)			Genus			DOC Notes		Dilution water
	Oligochaete Aeolosoma sp		48		6		025		Reproduction (population size)			1235			17098			17098		17098			Aeolosoma		Invert		Author reported half the detection limit
	Rotifer Brachionus calyciflorus		100		62		025		Reproduction (population size)			431			2555			2555		2555			Brachionus		Invert		Author reported half the detection limit
	Great pond snail Lymnaea stagnalis		117		61		025		Biomass			746			4877			4877		4877			Lymnaea		Mollusk		Author reported half the detection limit
	Fatmucket Lampsilis siliquoidea		1055		604		040		Biomass			169			1042			1042		1042			Lampsilis		Mollusk		Author reported
	Cladoceran Ceriodaphnia dubia		50		715		11		Reproduction - youngstarting adult			1780			1898						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		26		73		05		Reproduction - youngfemale			1557			2718						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		46		75		05		Reproduction - youngfemale			8087			1102						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		96		79		05		Reproduction - youngfemale			6519			796						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		194		81		05		Reproduction - youngfemale			6836			888						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		255		703		05		Reproduction (youngfemale)			250			563						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		122		713		05		Reproduction (youngfemale)			860			1061						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		25		798		05		Reproduction (youngfemale)			700			1006						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		61		803		05		Reproduction (youngfemale)			1010			1363						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		122		810		05		Reproduction (youngfemale)			870			1164						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		255		633		05		Reproduction (youngfemale)			260			1758						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		121		636		05		Reproduction (youngfemale)			390			1101			1182		1182			Ceriodaphnia		Invert		Author reported
	Amphipod Hyalella azteca		95		61		051		Biomass (28 d)			1993			1014						Hyalella			Author reported
	Amphipod Hyalella azteca		106		604		033		Biomass (28 d)			425			2895			1713		1713			Hyalella		Invert		Author reported
	Midge Chironomus riparius		118		558		18		Adult midge emergence			2955			1192						Chironomus			Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Midge Chironomus riparius		119		505		18		Adult midge emergence			8442			22578						Chironomus			Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Midge Chironomus riparius		91		66		051		Reproduction ( of eggscase)			3387			7664			5908		5908			Chironomus		Invert		Author reported
	Atlantic salmon Salmo salar		127		57		18		Biomass			6156			5085			5085		5085			Salmo		Fish		Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Brook trout Salvelinus fontinalis		123		655		19		Biomass			1644			270						Salvelinus			Author reported		wellRO mix
	Brook trout Salvelinus fontinalis		128		565		18		Biomass			1435			1295			5914		5914			Salvelinus		Fish		Author reported		wellRO mix
	Fathead minnow Pimephales promelas		220		770		16		Biomass			6194			3569						Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		well water
	Fathead minnow Pimephales promelas		96		620		025		Fry survival			4286			1734			2488		2488			Pimephales		Fish		Author reported half the detection limit
	Zebrafish Danio rerio		83		61		025		Biomass			2344			1102			1102		1102			Danio		Fish		Author reported half the detection limit
	Wood frog Rana sylvatica		115		469		16		MATC-survival		gt		2000		gt		9746		gt		9746		9746		gt		Rana		Amphib - Other Data		Value is from 2007 FW Cu AWQC Appendix C recommendation		aged tap water

	Appendix A Acceptable Acute Toxicity Data of Aluminum to Freshwater Aquatic Animals
	Species		Methoda		Hardness (mgL as CaCO3)		pH		DOC(mgL)			LC50 or EC50 Conc (microgL)		Reference			Normalized Conc - LC50(microgL)			Species Mean Acute Value(microgL)		Genus Mean Acute Value(microgL)			Genus			DOC Note		Dilution water
	Snail Physa sp		S M T		474		659		11		gt		23400		Call 1984 Call et al 1984		gt		48425			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		755		11			30600		Call 1984 Call et al 1984			26986			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		817		11		gt		24700		Call 1984 Call et al 1984		gt		24040			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		746		11			55500		Call 1984 Call et al 1984			50338			35462		35462			Physa		Mollusk		Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Fatmucket Lampsilis siliquoidea		F M T		1060		612		048		gt		6302		Wang et al 2016 Wang et al 2017		gt		29834		gt		29834		29834		gt		Lampsilis		Mollusk		Author reported
	Cladoceran Ceriodaphnia dubia		S M A		500		742		11			1900		McCauley et al 1986			1728			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		S M A		505		786		11			1500		McCauley et al 1986			1294			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		UW-S Lake Superior lab water
	Cladoceran Ceriodaphnia dubia		S M A		500		813		11			2560		McCauley et al 1986			2431			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		R M T		26		75		05			720		ENSR 1992d			1113			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		R M T		46		76		05			1880		ENSR 1992d			2495			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		R M T		96		78		05			2450		ENSR 1992d			2894			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		S U NR		90		715		05			3727		Fort and Stover 1995			5002			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U NR		90		715		05			5673		Fort and Stover 1995			7613			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U NR		89		76		05			2880		Soucek et al 2001			3362			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		601		05			7112		European Al Association 2009			1691			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		605		2			6865		European Al Association 2009			7088			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		609		4			15581		European Al Association 2009			10072			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		601		05			681		European Al Association 2009			1620			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		603		05			1630		European Al Association 2009			3674			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		597		05			1785		European Al Association 2009			4732			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		592		05			1410		European Al Association 2009			4295			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		699		05		gt		1300		European Al Association 2009		gt		4189			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		785		05		gt		5000		European Al Association 2009		gt		7960			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		68		2		gt		10000		European Al Association 2009		gt		20755			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		782		2		gt		15000		European Al Association 2009		gt		11626			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		677		4		gt		10000		European Al Association 2009		gt		15161			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		766		4		gt		15000		European Al Association 2009		gt		8608			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		79		05		gt		2000		European Al Association 2009		gt		3150			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		789		05		gt		2000		European Al Association 2009		gt		3156			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		604		05			1108		European Al Association 2009			850			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		598		2			11371		European Al Association 2009			4817			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		573		4			80467		European Al Association 2009			43570			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		671		05		gt		10000		European Al Association 2009		gt		23786			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		783		05		gt		5000		European Al Association 2009		gt		6344			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		679		2		gt		10000		European Al Association 2009		gt		10394			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		767		2		gt		15000		European Al Association 2009		gt		9082			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		668		4		gt		15000		European Al Association 2009		gt		12461			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		762		4		gt		15000		European Al Association 2009		gt		6332			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		606		2			33868		European Al Association 2009			7920			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		56		4			104842		European Al Association 2009			48800			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		693		05		gt		5000		European Al Association 2009		gt		7126			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		120		788		05		gt		5000		European Al Association 2009		gt		5898			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		120		676		2		gt		15000		European Al Association 2009		gt		12117			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		771		2		gt		15000		European Al Association 2009		gt		8096			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		66		4		gt		15000		European Al Association 2009		gt		10139			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		76		4		gt		15000		European Al Association 2009		gt		5545			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			11971		European Al Association 2010			2699			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			27478		European Al Association 2010			6194			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			11998		European Al Association 2010			2705			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		607		05			92495		European Al Association 2010			1876			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		609		05			31337		European Al Association 2010			6035			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		61		05			33235		European Al Association 2010			6238			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		708		05		gt		8864		European Al Association 2010		gt		2534			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		779		05		gt		42783		European Al Association 2010		gt		6930			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		753		05			13204		European Al Association 2010			244			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		600		601		05			46326		European Al Association 2010			3798			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		600		599		05		gt		8590		European Al Association 2010		gt		7366			5094				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia reticulata		F M T		451		60		11			304		Shephard 1983			2009			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia reticulata		F M T		40		55		11			362		Shephard 1983			55307			10542		7328			Ceriodaphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake SuperiorDI Mix
	Cladoceran Daphnia magna		S U NR		453		774		11			3900		Biesinger and Christensen 1972			3382			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Daphnia magna		S M T		220		76		16			38200		Kimball 1978			20303			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Well water
	Cladoceran Daphnia magna		S U T		451		725		11			2800		Shephard 1983			2868			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Daphnia magna		S U T		168		599		05			5000		European Al Association 2009		gt		2265			-				Daphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Daphnia magna		S U T		168		792		05			7950		European Al Association 2009			920			3332		3332			Daphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Ostracod Stenocypris major		R M T		1563		651		16			310196		Shuhaimi-Othman et al 2011a			10216			10216		10216			Stenocypris		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (Malaysia)
	Amphipod Crangonyx pseudogracilis		R U T		50		675		16			9190		Martin and Holdich 1986			12174			12174		12174			Crangonyx		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (UK)
	Amphipod Hyalella azteca		F M T		105		613		048		gt		5997		Wang et al 2016 Wang et al 2017		gt		28002		gt		28002		28002		gt		Hyalella		Invert		Author reported
	Stonefly Acroneuria sp		S M T		474		746		11		gt		22600		Call et al 1984		gt		20498		gt		20498		20498		gt		Acroneuria		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Midge Chironomus plumosus		S U T		80		70		16			30000		Fargasova 2001			25361			25361		25361			Chironomus		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (Slovakia)
	Midge Paratanytarsus dissimilis		S M T		1743		728		28		gt		77700		Lamb and Bailey 1981 1983		gt		62318		gt		62318		62318		gt		Paratanytarsus		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Liberty Lake Washington
	Rainbow trout Oncorhynchus mykiss		F M T		274		758		05		gt		9840		Gundersen et al 1994		gt		5745			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		452		762		05		gt		8070		Gundersen et al 1994		gt		5212			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		895		758		05		gt		8160		Gundersen et al 1994		gt		6807			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		1304		758		05		gt		8200		Gundersen et al 1994		gt		7662			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		232		825		05			6170		Gundersen et al 1994			1190			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		350		826		05			6170		Gundersen et al 1994			1523			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		836		829		05			7670		Gundersen et al 1994			3205			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		1158		830		05			6930		Gundersen et al 1994			3554			3661		3661			Oncorhynchus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Atlantic salmon Salmo salar		S U T		68		55		05			584		Hamilton and Haines 1995			21042			-				Salmo			Value is from 2007 FW Cu AWQC Appendix C recommendation		Soft recon
	Atlantic salmon Salmo salar		S U T		68		65		05			599		Hamilton and Haines 1995			2430			7151		7151			Salmo		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Soft recon
	Brook trout Salvelinus fontinalis		S U T		40		56		16			6530		Tandjung 1982			31767			-				Salvelinus			Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Brook trout Salvelinus fontinalis		S U T		18		56		16			3400		Tandjung 1982			28391			-				Salvelinus			Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Brook trout Salvelinus fontinalis		S U T		2		56		16			370		Tandjung 1982			13663			23097		23097			Salvelinus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Green sunfish Lepomis cyanellus		S M T		474		755		11		gt		50000		Call et al 1984		gt		24028		gt		24028		24028		gt		Lepomis		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Rio Grande silvery minnow Hybognathus amarus		R M T		140		81		05		gt		59100		Buhl 2002		gt		39414		gt		39414		39414		gt		Hybognathus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon water
	Fathead minnow Pimephales promelas		S M T		474		761		11		gt		48200		Call et al 1984		gt		21522			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Fathead minnow Pimephales promelas		S M T		474		805		11		gt		49800		Call et al 1984		gt		12972			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Fathead minnow Pimephales promelas		R M T		26		78		05			1160		ENSR 1992c			477			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		46		76		05			8180		ENSR 1992c			5446			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		96		81		05			20300		ENSR 1992c			10966			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		194		81		05			44800		ENSR 1992c			35847			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		140		81		05		gt		59100		Buhl 2002		gt		39414			10168		10168			Pimephales		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon water
	Smallmouth bass Micropterus dolomieui		S M T		1245		505		16			130		Kane and Rabeni 1987			3929			3929		3929			Micropterus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Green tree frog Hyla cinerea		R M T		455		549		05		gt		4052		Jung and Jagoe 1995		gt		20016		gt		20016		20016		gt		Hyla		Amphibian		Value is from 2007 FW Cu AWQC Appendix C recommendation		artificial soft recon

	Normalization Chemistry
	pH		7					FAV		2741
	Hardness		100					CMC		1400
	DOC		1					CCC		390
	Acute Ranked GMAV											Chronic Ranked GMCV
	Rank			GMAV		Genus								Rank			GMCV		Genus
	18		gt		62318		Paratanytarsus		Invert							12			17098		Aeolosoma		Invert
	17		gt		39414		Hybognathus		Fish							11		gt		9746		Rana		Amphib - Other Data
	16			35462		Physa		Mollusk							10			5908		Chironomus		Invert
	15		gt		29834		Lampsilis		Mollusk							9			4877		Lymnaea		Mollusk
	14		gt		28002		Hyalella		Invert							8			2555		Brachionus		Invert
	13			25361		Chironomus		Invert							7			2488		Pimephales		Fish
	12		gt		24028		Lepomis		Fish							6			1713		Hyalella		Invert
	11			23097		Salvelinus		Fish							5			1182		Ceriodaphnia		Invert
	10		gt		20498		Acroneuria		Invert							4			1102		Danio		Fish
	9		gt		20016		Hyla		Amphibian							3			1042		Lampsilis		Mollusk
	8			12174		Crangonyx		Invert							2			591		Salvelinus		Fish
	7			10216		Stenocypris		Invert							1			509		Salmo		Fish
	6			10168		Pimephales		Fish
	5			7328		Ceriodaphnia		Invert
	4			7151		Salmo		Fish
	3			3929		Micropterus		Fish
	2			3661		Oncorhynchus		Fish
	1			3332		Daphnia		Invert
		N		Rank		Genus		GMAV		ln(GMAV)		ln(GMAV)2		P=R(N+1)		sqrt(P)				N		Rank		Genus		GMCV		ln(GMCV)		ln(GMCV)2		P=R(N+1)		sqrt(P)
		18		4		Salmo		7151		887		7876		0211		0459				12		4		Danio		1102		700		4907		0308		0555
			3		Micropterus		3929		828		6849		0158		0397					3		Lampsilis		1042		695		4829		0231		0480
			2		Oncorhynchus		3661		821		6733		0105		0324					2		Salvelinus		591		638		4074		0154		0392
			1		Daphnia		3332		811		6579		0053		0229					1		Salmo		509		623		3883		0077		0277
			Sum				3347		2804		0526		141					Sum				2657		1769		0769		170
			S2 =		1223										S2 =		1080
			L =		7134										L =		5242
			A =		7916										A =		5976
			FAV =		2741										FCV =		3940
			CMC =		1371

				All concentrations reported are microgL total Aluminum
				lt-----Click Button after entering data
	ENTER DATA HERE									ERRORNUM		ACUTE														CHRONIC
										Rank 1				Rank 2				Rank 3				Rank 4					Rank 1				Rank 2				Rank 3				Rank 4
	SiteName		DOC(mgL)		Hardness(mgL as CaCO3)		pH			FAV		CMC		CCC			Genus		GMAV			Genus		GMAV			Genus		GMAV			Genus		GMAV				Genus		GMCV			Genus		GMCV			Genus		GMCV			Genus		GMCV
	Site 1		50		25		9		75428737034162		35229324666008		1800		770			Daphnia			33452		Ceriodaphnia			73576		Stenocypris			102576		Crangonyx			122230			Lampsilis			10463		Ceriodaphnia			11865		Hyalella			17200		Brachionus			25648
	Site 2		50		50		9		75428737034162		31204991509063		1600		680			Daphnia			29631		Ceriodaphnia			65171		Stenocypris			90859		Crangonyx			108267			Lampsilis			9268		Ceriodaphnia			10510		Hyalella			15236		Brachionus			22718
	Site 3		50		75		9		75428737034162		29067527928199		1500		640			Daphnia			27601		Ceriodaphnia			60707		Stenocypris			84635		Crangonyx			100851			Lampsilis			8633		Ceriodaphnia			9790		Hyalella			14192		Brachionus			21162
	Site 4		50		100		9		75428737034162		27640367912592		1400		600			Daphnia			26246		Ceriodaphnia			57726		Stenocypris			80480		Crangonyx			95899			Lampsilis			8209		Ceriodaphnia			9309		Hyalella			13495		Brachionus			20123
	Site 5		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 6		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 7		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 8		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 9		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 10		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 11		50		25		85		75428737034162		63245275664384		3200		1400			Daphnia			60055		Ceriodaphnia			132086		Stenocypris			184149		Crangonyx			219432			Lampsilis			18783		Ceriodaphnia			21301		Hyalella			30879		Brachionus			46045
	Site 12		50		50		85		75428737034162		61388044609926		3100		1300			Daphnia			58291		Ceriodaphnia			128208		Stenocypris			178742		Crangonyx			212988			Lampsilis			18232		Ceriodaphnia			20676		Hyalella			29972		Brachionus			44693
	Site 13		50		75		85		75428737034162		60327020429132		3000		1300			Daphnia			57284		Ceriodaphnia			125992		Stenocypris			175652		Crangonyx			209307			Lampsilis			17917		Ceriodaphnia			20318		Hyalella			29454		Brachionus			43920
	Site 14		50		100		85		75428737034162		59585352130146		3000		1300			Daphnia			56580		Ceriodaphnia			124443		Stenocypris			173493		Crangonyx			206734			Lampsilis			17696		Ceriodaphnia			20068		Hyalella			29092		Salmo			41264
	Site 15		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 16		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 17		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 18		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 19		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 20		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370

IV Construct sensitivity distribution to determine criteria at set conditions

22

Figure 10 Ranked Summary of Total Aluminum Genus Mean Chronic Values (GMCVs) ndash Freshwater Supplemented with Other Data to Fulfill Missing MDRs at pH 7 Hardness of 100 mgL and DOC of 1 mgL

Aluminum Criteria Calculatorbull The relative ranking of genera in the sensitivity

distribution to which the toxicity data are normalized will change based on the set of water chemistry conditions (ie pH hardness and DOC)

bull To make calculating aluminum criteria easier EPA developed an Aluminum Criteria Calculatorndash Automated so that user only needs to enter water chemistry

conditions of interestndash Allows quick calculations for a variety of conditionsndash Limits calculations to the recommended conditions for which

the MLR models were developed were specified for hardness and DOC

bull No extrapolation beyond hardness and DOC empirical data bounds

23

Aluminum Criteria Calculator SD Example

24

pH=7 Hardness=100 DOC=1 pH=6 Hardness=25 DOC=1

Chronic Ranked GMCVRank GMCV Genus

12 17098 Aeolosoma Invert11 gt 9746 Rana Amphib - Other Data10 5908 Chironomus Invert

9 4877 Lymnaea Mollusk8 2555 Brachionus Invert7 2488 Pimephales Fish6 1713 Hyalella Invert5 1182 Ceriodaphnia Invert4 1102 Danio Fish3 1042 Lampsilis Mollusk2 591 Salvelinus Fish1 509 Salmo Fish

FCV = 3940 microgL total aluminum

Chronic Ranked GMCVRank GMCV Genus

12 gt 2131 Rana Amphib - Other Data11 1710 Aeolosoma Invert10 591 Chironomus Invert

9 544 Pimephales Fish8 488 Lymnaea Mollusk7 255 Brachionus Invert6 241 Danio Fish5 171 Hyalella Invert4 129 Salvelinus Fish3 118 Ceriodaphnia Invert2 111 Salmo Fish1 104 Lampsilis Mollusk

FCV = 987 microgL total aluminum

Multiple Scenarios

Run Scenarios

Summary Sheet

Acute Dataset

Chronic Dataset

Multiple Scenarios

Run Scenarios

Summary Sheet

Acute Dataset

Chronic Dataset

Boundaries of the Criteria Outputsbull MLR models were developed using data that

encompassndash DOC range of 008-5 mgLndash Hardness range of 98-127 mgL (as CaCO3)ndash pH range of 6-81

bull MLR criteria outputs are bounded atndash Maximum of 150 mgL total hardness (as CaCO3)

ndash DOC of 50 mgLndash pH range of the model is 50 to 90

25

Aluminum Criteria Look-Up Tables

26

bull EPA also provided criteria look-up tables in Appendix K

Har

dnes

s

CCC(microgL total aluminum)

(DOC=05 mgL)

pH 50 pH 55 pH 60 pH 65 pH 70 pH 75 pH 80 pH 85 pH 90

25 23 a 14 b 69 c 160 f 350 f 620 i 540 b 410 a 230 a

50 43 a 24 b 100 d 170 f 310 f 630 f 670 j 400 a 200 a

75 61 a 33 b 130 e 180 f 290 f 530 f 730 i 390 a 190 a

100 78 a 41 b 150 e 190 f 280 g 460 f 770 c 390 a 180 a

150 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

200 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

250 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

300 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

350 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

400 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

Aluminum Criteria Look-Up Tables

27

bull EPA also provided criteria look-up tables in Appendix K

Har

dnes

s

CCC(microgL total aluminum)

(DOC=10 mgL)

pH 50 pH 55 pH 60 pH 65 pH 70 pH 75 pH 80 pH 85 pH 90

25 33 a 21 b 99 c 220 f 490 f 900 i 780 b 590 a 330 a

50 61 a 35 b 150 e 250 f 440 f 890 f 960 j 580 a 290 a

75 88 a 47 b 180 e 260 f 410 f 740 f 1100 i 570 a 270 a

100 11 a 59 b 210 e 270 f 390 g 650 f 1100 c 560 b 260 a

150 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

200 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

250 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

300 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

350 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

400 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

Aluminum Criteria Look-Up Tables

28

bull EPA also provided criteria look-up tables in Appendix K

Har

dnes

s

CCC(microgL total aluminum)

(DOC=25 mgL)

pH 50 pH 55 pH 60 pH 65 pH 70 pH 75 pH 80 pH 85 pH 90

25 54 a 34 b 160 c 350 f 770 f 1500 i 1300 b 960 a 540 a

50 99 a 57 b 230 e 390 f 690 f 1400 f 1600 j 930 a 470 a

75 14 a 77 b 290 e 410 f 640 f 1200 f 1700 i 920 a 440 a

100 18 a 96 b 330 e 420 f 620 g 1000 f 1800 d 920 b 420 a

150 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

200 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

250 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

300 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

350 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

400 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

Aluminum Criteria Look-Up Tables

29

bull EPA also provided criteria look-up tables in Appendix K

Har

dnes

s

CCC(microgL total aluminum)

(DOC=50 mgL)

pH 50 pH 55 pH 60 pH 65 pH 70 pH 75 pH 80 pH 85 pH 90

25 77 a 49 b 230 c 500 f 1100 f 2100 c 1900 b 1400 a 770 a

50 14 a 82 b 330 e 540 f 970 f 2000 f 2200 j 1300 a 680 a

75 20 a 110 b 410 e 570 f 900 f 1600 f 2500 i 1300 a 640 a

100 26 a 140 b 460 f 590 f 880 g 1400 f 2500 d 1300 b 600 a

150 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

200 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

250 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

300 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

350 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

400 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

Status

30

bull Draft Document Released to the Federal Register on 72817ndash Public comment period of 60 days ndash Due Date for Comments is 92617

bull Revisions will be made considering public commentsbull Subsequent EPA intra-agency review of final criteria

document will then be conducted bull Publication of final criteria document planned for Late Fall

2017

Federal Register Webpagehttpswwwfederalregistergovdocuments201

707282017-15968request-for-scientific-views-draft-updated-aquatic-life-ambient-water-quality-criteria-for-aluminum

Aluminum Criteria Webpagehttpswwwepagovwqc2017-draft-aquatic-life-

criteria-aluminum-freshwater-documents

31

	Appendix C Acceptable Chronic Toxicity Data of Aluminum to Freshwater Aquatic Animals
									MLR equation
	Species		Hardness (mgL as CaCO3)		pH		DOC(mgL)		EC20 Endpoint			EC20(microgL)			Normalized Conc- EC20(microgL)			SMCV-EC20(microgL)		GMCV-EC20(microgL)			Genus			DOC Notes		Dilution water
	Oligochaete Aeolosoma sp		48		6		025		Reproduction (population size)			1235			1710			1710		1710			Aeolosoma		Invert		Author reported half the detection limit
	Rotifer Brachionus calyciflorus		100		62		025		Reproduction (population size)			431			255			255		255			Brachionus		Invert		Author reported half the detection limit
	Great pond snail Lymnaea stagnalis		117		61		025		Biomass			746			488			488		488			Lymnaea		Mollusk		Author reported half the detection limit
	Fatmucket Lampsilis siliquoidea		1055		604		040		Biomass			169			104			104		104			Lampsilis		Mollusk		Author reported
	Cladoceran Ceriodaphnia dubia		50		715		11		Reproduction - youngstarting adult			1780			190						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		26		73		05		Reproduction - youngfemale			1557			272						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		46		75		05		Reproduction - youngfemale			8087			110						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		96		79		05		Reproduction - youngfemale			6519			80						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		194		81		05		Reproduction - youngfemale			6836			89						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		255		703		05		Reproduction (youngfemale)			250			56						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		122		713		05		Reproduction (youngfemale)			860			106						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		25		798		05		Reproduction (youngfemale)			700			101						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		61		803		05		Reproduction (youngfemale)			1010			136						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		122		810		05		Reproduction (youngfemale)			870			116						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		255		633		05		Reproduction (youngfemale)			260			176						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		121		636		05		Reproduction (youngfemale)			390			110			118		118			Ceriodaphnia		Invert		Author reported
	Amphipod Hyalella azteca		95		61		051		Biomass (28 d)			1993			101						Hyalella			Author reported
	Amphipod Hyalella azteca		106		604		033		Biomass (28 d)			425			289			171		171			Hyalella		Invert		Author reported
	Midge Chironomus riparius		118		558		18		Adult midge emergence			2955			119						Chironomus			Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Midge Chironomus riparius		119		505		18		Adult midge emergence			8442			2258						Chironomus			Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Midge Chironomus riparius		91		66		051		Reproduction ( of eggscase)			3387			766			591		591			Chironomus		Invert		Author reported
	Atlantic salmon Salmo salar		127		57		18		Biomass			6156			1112			1112		1112			Salmo		Fish		Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Brook trout Salvelinus fontinalis		123		655		19		Biomass			1644			59						Salvelinus			Author reported		wellRO mix
	Brook trout Salvelinus fontinalis		128		565		18		Biomass			1435			283			1293		1293			Salvelinus		Fish		Author reported		wellRO mix
	Fathead minnow Pimephales promelas		220		770		16		Biomass			6194			781						Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		well water
	Fathead minnow Pimephales promelas		96		620		025		Fry survival			4286			379			544		544			Pimephales		Fish		Author reported half the detection limit
	Zebrafish Danio rerio		83		61		025		Biomass			2344			241			241		241			Danio		Fish		Author reported half the detection limit
	Wood frog Rana sylvatica		115		469		16		MATC-survival		gt		2000		gt		2131		gt		2131		2131		gt		Rana		Amphib - Other Data		Value is from 2007 FW Cu AWQC Appendix C recommendation		aged tap water

	Appendix A Acceptable Acute Toxicity Data of Aluminum to Freshwater Aquatic Animals
	Species		Methoda		Hardness (mgL as CaCO3)		pH		DOC(mgL)			LC50 or EC50 Conc (microgL)		Reference			Normalized Conc - LC50(microgL)			Species Mean Acute Value(microgL)		Genus Mean Acute Value(microgL)			Genus			DOC Note		Dilution water
	Snail Physa sp		S M T		474		659		11		gt		23400		Call 1984 Call et al 1984		gt		4843			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		755		11			30600		Call 1984 Call et al 1984			2699			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		817		11		gt		24700		Call 1984 Call et al 1984		gt		2404			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		746		11			55500		Call 1984 Call et al 1984			5034			3546		3546			Physa		Mollusk		Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Fatmucket Lampsilis siliquoidea		F M T		1060		612		048		gt		6302		Wang et al 2016 Wang et al 2017		gt		2983		gt		2983		2983		gt		Lampsilis		Mollusk		Author reported
	Cladoceran Ceriodaphnia dubia		S M A		500		742		11			1900		McCauley et al 1986			173			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		S M A		505		786		11			1500		McCauley et al 1986			129			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		UW-S Lake Superior lab water
	Cladoceran Ceriodaphnia dubia		S M A		500		813		11			2560		McCauley et al 1986			243			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		R M T		26		75		05			720		ENSR 1992d			111			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		R M T		46		76		05			1880		ENSR 1992d			250			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		R M T		96		78		05			2450		ENSR 1992d			289			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		S U NR		90		715		05			3727		Fort and Stover 1995			500			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U NR		90		715		05			5673		Fort and Stover 1995			761			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U NR		89		76		05			2880		Soucek et al 2001			336			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		601		05			7112		European Al Association 2009			169			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		605		2			6865		European Al Association 2009			709			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		609		4			15581		European Al Association 2009			1007			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		601		05			681		European Al Association 2009			162			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		603		05			1630		European Al Association 2009			367			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		597		05			1785		European Al Association 2009			473			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		592		05			1410		European Al Association 2009			429			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		699		05		gt		1300		European Al Association 2009		gt		419			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		785		05		gt		5000		European Al Association 2009		gt		796			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		68		2		gt		10000		European Al Association 2009		gt		2076			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		782		2		gt		15000		European Al Association 2009		gt		1163			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		677		4		gt		10000		European Al Association 2009		gt		1516			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		766		4		gt		15000		European Al Association 2009		gt		861			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		79		05		gt		2000		European Al Association 2009		gt		315			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		789		05		gt		2000		European Al Association 2009		gt		316			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		604		05			1108		European Al Association 2009			85			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		598		2			11371		European Al Association 2009			482			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		573		4			80467		European Al Association 2009			4357			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		671		05		gt		10000		European Al Association 2009		gt		2379			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		783		05		gt		5000		European Al Association 2009		gt		634			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		679		2		gt		10000		European Al Association 2009		gt		1039			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		767		2		gt		15000		European Al Association 2009		gt		908			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		668		4		gt		15000		European Al Association 2009		gt		1246			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		762		4		gt		15000		European Al Association 2009		gt		633			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		606		2			33868		European Al Association 2009			792			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		56		4			104842		European Al Association 2009			4880			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		693		05		gt		5000		European Al Association 2009		gt		713			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		120		788		05		gt		5000		European Al Association 2009		gt		590			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		120		676		2		gt		15000		European Al Association 2009		gt		1212			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		771		2		gt		15000		European Al Association 2009		gt		810			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		66		4		gt		15000		European Al Association 2009		gt		1014			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		76		4		gt		15000		European Al Association 2009		gt		555			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			11971		European Al Association 2010			270			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			27478		European Al Association 2010			619			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			11998		European Al Association 2010			270			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		607		05			92495		European Al Association 2010			188			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		609		05			31337		European Al Association 2010			604			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		61		05			33235		European Al Association 2010			624			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		708		05		gt		8864		European Al Association 2010		gt		253			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		779		05		gt		42783		European Al Association 2010		gt		693			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		753		05			13204		European Al Association 2010			24			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		600		601		05			46326		European Al Association 2010			380			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		600		599		05		gt		8590		European Al Association 2010		gt		737			509				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia reticulata		F M T		451		60		11			304		Shephard 1983			201			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia reticulata		F M T		40		55		11			362		Shephard 1983			5531			1054		733			Ceriodaphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake SuperiorDI Mix
	Cladoceran Daphnia magna		S U NR		453		774		11			3900		Biesinger and Christensen 1972			338			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Daphnia magna		S M T		220		76		16			38200		Kimball 1978			2030			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Well water
	Cladoceran Daphnia magna		S U T		451		725		11			2800		Shephard 1983			287			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Daphnia magna		S U T		168		599		05			5000		European Al Association 2009		gt		227			-				Daphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Daphnia magna		S U T		168		792		05			7950		European Al Association 2009			92			333		333			Daphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Ostracod Stenocypris major		R M T		1563		651		16			310196		Shuhaimi-Othman et al 2011a			1022			1022		1022			Stenocypris		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (Malaysia)
	Amphipod Crangonyx pseudogracilis		R U T		50		675		16			9190		Martin and Holdich 1986			1217			1217		1217			Crangonyx		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (UK)
	Amphipod Hyalella azteca		F M T		105		613		048		gt		5997		Wang et al 2016 Wang et al 2017		gt		2800		gt		2800		2800		gt		Hyalella		Invert		Author reported
	Stonefly Acroneuria sp		S M T		474		746		11		gt		22600		Call et al 1984		gt		2050		gt		2050		2050		gt		Acroneuria		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Midge Chironomus plumosus		S U T		80		70		16			30000		Fargasova 2001			2536			2536		2536			Chironomus		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (Slovakia)
	Midge Paratanytarsus dissimilis		S M T		1743		728		28		gt		77700		Lamb and Bailey 1981 1983		gt		6232		gt		6232		6232		gt		Paratanytarsus		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Liberty Lake Washington
	Rainbow trout Oncorhynchus mykiss		F M T		274		758		05		gt		9840		Gundersen et al 1994		gt		1256			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		452		762		05		gt		8070		Gundersen et al 1994		gt		1140			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		895		758		05		gt		8160		Gundersen et al 1994		gt		1489			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		1304		758		05		gt		8200		Gundersen et al 1994		gt		1676			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		232		825		05			6170		Gundersen et al 1994			260			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		350		826		05			6170		Gundersen et al 1994			333			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		836		829		05			7670		Gundersen et al 1994			701			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		1158		830		05			6930		Gundersen et al 1994			777			801		801			Oncorhynchus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Atlantic salmon Salmo salar		S U T		68		55		05			584		Hamilton and Haines 1995			4602			-				Salmo			Value is from 2007 FW Cu AWQC Appendix C recommendation		Soft recon
	Atlantic salmon Salmo salar		S U T		68		65		05			599		Hamilton and Haines 1995			531			1564		1564			Salmo		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Soft recon
	Brook trout Salvelinus fontinalis		S U T		40		56		16			6530		Tandjung 1982			6947			-				Salvelinus			Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Brook trout Salvelinus fontinalis		S U T		18		56		16			3400		Tandjung 1982			6209			-				Salvelinus			Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Brook trout Salvelinus fontinalis		S U T		2		56		16			370		Tandjung 1982			2988			5051		5051			Salvelinus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Green sunfish Lepomis cyanellus		S M T		474		755		11		gt		50000		Call et al 1984		gt		5255		gt		5255		5255		gt		Lepomis		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Rio Grande silvery minnow Hybognathus amarus		R M T		140		81		05		gt		59100		Buhl 2002		gt		8620		gt		8620		8620		gt		Hybognathus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon water
	Fathead minnow Pimephales promelas		S M T		474		761		11		gt		48200		Call et al 1984		gt		4707			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Fathead minnow Pimephales promelas		S M T		474		805		11		gt		49800		Call et al 1984		gt		2837			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Fathead minnow Pimephales promelas		R M T		26		78		05			1160		ENSR 1992c			104			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		46		76		05			8180		ENSR 1992c			1191			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		96		81		05			20300		ENSR 1992c			2398			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		194		81		05			44800		ENSR 1992c			7839			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		140		81		05		gt		59100		Buhl 2002		gt		8620			2224		2224			Pimephales		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon water
	Smallmouth bass Micropterus dolomieui		S M T		1245		505		16			130		Kane and Rabeni 1987			859			859		859			Micropterus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Green tree frog Hyla cinerea		R M T		455		549		05		gt		4052		Jung and Jagoe 1995		gt		4377		gt		4377		4377		gt		Hyla		Amphibian		Value is from 2007 FW Cu AWQC Appendix C recommendation		artificial soft recon

	Normalization Chemistry
	pH		6					FAV		360
	Hardness		25					CMC		180
	DOC		1					CCC		99
	Acute Ranked GMAV											Chronic Ranked GMCV
	Rank			GMAV		Genus								Rank			GMCV		Genus
	18		gt		8620		Hybognathus		Fish							12		gt		2131		Rana		Amphib - Other Data
	17		gt		6232		Paratanytarsus		Invert							11			1710		Aeolosoma		Invert
	16		gt		5255		Lepomis		Fish							10			591		Chironomus		Invert
	15			5051		Salvelinus		Fish							9			544		Pimephales		Fish
	14		gt		4377		Hyla		Amphibian							8			488		Lymnaea		Mollusk
	13			3546		Physa		Mollusk							7			255		Brachionus		Invert
	12		gt		2983		Lampsilis		Mollusk							6			241		Danio		Fish
	11		gt		2800		Hyalella		Invert							5			171		Hyalella		Invert
	10			2536		Chironomus		Invert							4			129		Salvelinus		Fish
	9			2224		Pimephales		Fish							3			118		Ceriodaphnia		Invert
	8		gt		2050		Acroneuria		Invert							2			111		Salmo		Fish
	7			1564		Salmo		Fish							1			104		Lampsilis		Mollusk
	6			1217		Crangonyx		Invert
	5			1022		Stenocypris		Invert
	4			859		Micropterus		Fish
	3			801		Oncorhynchus		Fish
	2			733		Ceriodaphnia		Invert
	1			333		Daphnia		Invert
		N		Rank		Genus		GMAV		ln(GMAV)		ln(GMAV)2		P=R(N+1)		sqrt(P)				N		Rank		Genus		GMCV		ln(GMCV)		ln(GMCV)2		P=R(N+1)		sqrt(P)
		18		4		Micropterus		859		676		4564		0211		0459				12		4		Salvelinus		129		486		2364		0308		0555
			3		Oncorhynchus		801		669		4469		0158		0397					3		Ceriodaphnia		118		477		2277		0231		0480
			2		Ceriodaphnia		733		660		4352		0105		0324					2		Salmo		111		471		2220		0154		0392
			1		Daphnia		333		581		3374		0053		0229					1		Lampsilis		104		465		2159		0077		0277
			Sum				2585		1676		0526		141					Sum				1899		902		0769		170
			S2 =		1987										S2 =		059
			L =		4890										L =		4420
			A =		5887										A =		4592
			FAV =		360										FCV =		987
			CMC =		180

				All concentrations reported are microgL total Aluminum
				lt-----Click Button after entering data
	ENTER DATA HERE									ERRORNUM		ACUTE														CHRONIC
										Rank 1				Rank 2				Rank 3				Rank 4					Rank 1				Rank 2				Rank 3				Rank 4
	SiteName		DOC(mgL)		Hardness(mgL as CaCO3)		pH			FAV		CMC		CCC			Genus		GMAV			Genus		GMAV			Genus		GMAV			Genus		GMAV				Genus		GMCV			Genus		GMCV			Genus		GMCV			Genus		GMCV
	Site 1		50		25		9		75428737034162		35229324666008		1800		770			Daphnia			33452		Ceriodaphnia			73576		Stenocypris			102576		Crangonyx			122230			Lampsilis			10463		Ceriodaphnia			11865		Hyalella			17200		Brachionus			25648
	Site 2		50		50		9		75428737034162		31204991509063		1600		680			Daphnia			29631		Ceriodaphnia			65171		Stenocypris			90859		Crangonyx			108267			Lampsilis			9268		Ceriodaphnia			10510		Hyalella			15236		Brachionus			22718
	Site 3		50		75		9		75428737034162		29067527928199		1500		640			Daphnia			27601		Ceriodaphnia			60707		Stenocypris			84635		Crangonyx			100851			Lampsilis			8633		Ceriodaphnia			9790		Hyalella			14192		Brachionus			21162
	Site 4		50		100		9		75428737034162		27640367912592		1400		600			Daphnia			26246		Ceriodaphnia			57726		Stenocypris			80480		Crangonyx			95899			Lampsilis			8209		Ceriodaphnia			9309		Hyalella			13495		Brachionus			20123
	Site 5		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 6		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 7		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 8		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 9		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 10		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 11		50		25		85		75428737034162		63245275664384		3200		1400			Daphnia			60055		Ceriodaphnia			132086		Stenocypris			184149		Crangonyx			219432			Lampsilis			18783		Ceriodaphnia			21301		Hyalella			30879		Brachionus			46045
	Site 12		50		50		85		75428737034162		61388044609926		3100		1300			Daphnia			58291		Ceriodaphnia			128208		Stenocypris			178742		Crangonyx			212988			Lampsilis			18232		Ceriodaphnia			20676		Hyalella			29972		Brachionus			44693
	Site 13		50		75		85		75428737034162		60327020429132		3000		1300			Daphnia			57284		Ceriodaphnia			125992		Stenocypris			175652		Crangonyx			209307			Lampsilis			17917		Ceriodaphnia			20318		Hyalella			29454		Brachionus			43920
	Site 14		50		100		85		75428737034162		59585352130146		3000		1300			Daphnia			56580		Ceriodaphnia			124443		Stenocypris			173493		Crangonyx			206734			Lampsilis			17696		Ceriodaphnia			20068		Hyalella			29092		Salmo			41264
	Site 15		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 16		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 17		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 18		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 19		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 20		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370

	Appendix C Acceptable Chronic Toxicity Data of Aluminum to Freshwater Aquatic Animals
									MLR equation
	Species		Hardness (mgL as CaCO3)		pH		DOC(mgL)		EC20 Endpoint			EC20(microgL)			Normalized Conc- EC20(microgL)			SMCV-EC20(microgL)		GMCV-EC20(microgL)			Genus			DOC Notes		Dilution water
	Oligochaete Aeolosoma sp		48		6		025		Reproduction (population size)			1235			17098			17098		17098			Aeolosoma		Invert		Author reported half the detection limit
	Rotifer Brachionus calyciflorus		100		62		025		Reproduction (population size)			431			2555			2555		2555			Brachionus		Invert		Author reported half the detection limit
	Great pond snail Lymnaea stagnalis		117		61		025		Biomass			746			4877			4877		4877			Lymnaea		Mollusk		Author reported half the detection limit
	Fatmucket Lampsilis siliquoidea		1055		604		040		Biomass			169			1042			1042		1042			Lampsilis		Mollusk		Author reported
	Cladoceran Ceriodaphnia dubia		50		715		11		Reproduction - youngstarting adult			1780			1898						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		26		73		05		Reproduction - youngfemale			1557			2718						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		46		75		05		Reproduction - youngfemale			8087			1102						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		96		79		05		Reproduction - youngfemale			6519			796						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		194		81		05		Reproduction - youngfemale			6836			888						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		255		703		05		Reproduction (youngfemale)			250			563						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		122		713		05		Reproduction (youngfemale)			860			1061						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		25		798		05		Reproduction (youngfemale)			700			1006						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		61		803		05		Reproduction (youngfemale)			1010			1363						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		122		810		05		Reproduction (youngfemale)			870			1164						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		255		633		05		Reproduction (youngfemale)			260			1758						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		121		636		05		Reproduction (youngfemale)			390			1101			1182		1182			Ceriodaphnia		Invert		Author reported
	Amphipod Hyalella azteca		95		61		051		Biomass (28 d)			1993			1014						Hyalella			Author reported
	Amphipod Hyalella azteca		106		604		033		Biomass (28 d)			425			2895			1713		1713			Hyalella		Invert		Author reported
	Midge Chironomus riparius		118		558		18		Adult midge emergence			2955			1192						Chironomus			Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Midge Chironomus riparius		119		505		18		Adult midge emergence			8442			22578						Chironomus			Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Midge Chironomus riparius		91		66		051		Reproduction ( of eggscase)			3387			7664			5908		5908			Chironomus		Invert		Author reported
	Atlantic salmon Salmo salar		127		57		18		Biomass			6156			5085			5085		5085			Salmo		Fish		Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Brook trout Salvelinus fontinalis		123		655		19		Biomass			1644			270						Salvelinus			Author reported		wellRO mix
	Brook trout Salvelinus fontinalis		128		565		18		Biomass			1435			1295			5914		5914			Salvelinus		Fish		Author reported		wellRO mix
	Fathead minnow Pimephales promelas		220		770		16		Biomass			6194			3569						Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		well water
	Fathead minnow Pimephales promelas		96		620		025		Fry survival			4286			1734			2488		2488			Pimephales		Fish		Author reported half the detection limit
	Zebrafish Danio rerio		83		61		025		Biomass			2344			1102			1102		1102			Danio		Fish		Author reported half the detection limit
	Wood frog Rana sylvatica		115		469		16		MATC-survival		gt		2000		gt		9746		gt		9746		9746		gt		Rana		Amphib - Other Data		Value is from 2007 FW Cu AWQC Appendix C recommendation		aged tap water

	Appendix A Acceptable Acute Toxicity Data of Aluminum to Freshwater Aquatic Animals
	Species		Methoda		Hardness (mgL as CaCO3)		pH		DOC(mgL)			LC50 or EC50 Conc (microgL)		Reference			Normalized Conc - LC50(microgL)			Species Mean Acute Value(microgL)		Genus Mean Acute Value(microgL)			Genus			DOC Note		Dilution water
	Snail Physa sp		S M T		474		659		11		gt		23400		Call 1984 Call et al 1984		gt		48425			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		755		11			30600		Call 1984 Call et al 1984			26986			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		817		11		gt		24700		Call 1984 Call et al 1984		gt		24040			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		746		11			55500		Call 1984 Call et al 1984			50338			35462		35462			Physa		Mollusk		Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Fatmucket Lampsilis siliquoidea		F M T		1060		612		048		gt		6302		Wang et al 2016 Wang et al 2017		gt		29834		gt		29834		29834		gt		Lampsilis		Mollusk		Author reported
	Cladoceran Ceriodaphnia dubia		S M A		500		742		11			1900		McCauley et al 1986			1728			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		S M A		505		786		11			1500		McCauley et al 1986			1294			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		UW-S Lake Superior lab water
	Cladoceran Ceriodaphnia dubia		S M A		500		813		11			2560		McCauley et al 1986			2431			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		R M T		26		75		05			720		ENSR 1992d			1113			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		R M T		46		76		05			1880		ENSR 1992d			2495			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		R M T		96		78		05			2450		ENSR 1992d			2894			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		S U NR		90		715		05			3727		Fort and Stover 1995			5002			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U NR		90		715		05			5673		Fort and Stover 1995			7613			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U NR		89		76		05			2880		Soucek et al 2001			3362			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		601		05			7112		European Al Association 2009			1691			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		605		2			6865		European Al Association 2009			7088			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		609		4			15581		European Al Association 2009			10072			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		601		05			681		European Al Association 2009			1620			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		603		05			1630		European Al Association 2009			3674			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		597		05			1785		European Al Association 2009			4732			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		592		05			1410		European Al Association 2009			4295			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		699		05		gt		1300		European Al Association 2009		gt		4189			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		785		05		gt		5000		European Al Association 2009		gt		7960			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		68		2		gt		10000		European Al Association 2009		gt		20755			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		782		2		gt		15000		European Al Association 2009		gt		11626			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		677		4		gt		10000		European Al Association 2009		gt		15161			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		766		4		gt		15000		European Al Association 2009		gt		8608			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		79		05		gt		2000		European Al Association 2009		gt		3150			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		789		05		gt		2000		European Al Association 2009		gt		3156			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		604		05			1108		European Al Association 2009			850			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		598		2			11371		European Al Association 2009			4817			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		573		4			80467		European Al Association 2009			43570			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		671		05		gt		10000		European Al Association 2009		gt		23786			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		783		05		gt		5000		European Al Association 2009		gt		6344			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		679		2		gt		10000		European Al Association 2009		gt		10394			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		767		2		gt		15000		European Al Association 2009		gt		9082			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		668		4		gt		15000		European Al Association 2009		gt		12461			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		762		4		gt		15000		European Al Association 2009		gt		6332			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		606		2			33868		European Al Association 2009			7920			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		56		4			104842		European Al Association 2009			48800			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		693		05		gt		5000		European Al Association 2009		gt		7126			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		120		788		05		gt		5000		European Al Association 2009		gt		5898			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		120		676		2		gt		15000		European Al Association 2009		gt		12117			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		771		2		gt		15000		European Al Association 2009		gt		8096			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		66		4		gt		15000		European Al Association 2009		gt		10139			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		76		4		gt		15000		European Al Association 2009		gt		5545			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			11971		European Al Association 2010			2699			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			27478		European Al Association 2010			6194			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			11998		European Al Association 2010			2705			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		607		05			92495		European Al Association 2010			1876			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		609		05			31337		European Al Association 2010			6035			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		61		05			33235		European Al Association 2010			6238			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		708		05		gt		8864		European Al Association 2010		gt		2534			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		779		05		gt		42783		European Al Association 2010		gt		6930			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		753		05			13204		European Al Association 2010			244			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		600		601		05			46326		European Al Association 2010			3798			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		600		599		05		gt		8590		European Al Association 2010		gt		7366			5094				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia reticulata		F M T		451		60		11			304		Shephard 1983			2009			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia reticulata		F M T		40		55		11			362		Shephard 1983			55307			10542		7328			Ceriodaphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake SuperiorDI Mix
	Cladoceran Daphnia magna		S U NR		453		774		11			3900		Biesinger and Christensen 1972			3382			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Daphnia magna		S M T		220		76		16			38200		Kimball 1978			20303			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Well water
	Cladoceran Daphnia magna		S U T		451		725		11			2800		Shephard 1983			2868			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Daphnia magna		S U T		168		599		05			5000		European Al Association 2009		gt		2265			-				Daphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Daphnia magna		S U T		168		792		05			7950		European Al Association 2009			920			3332		3332			Daphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Ostracod Stenocypris major		R M T		1563		651		16			310196		Shuhaimi-Othman et al 2011a			10216			10216		10216			Stenocypris		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (Malaysia)
	Amphipod Crangonyx pseudogracilis		R U T		50		675		16			9190		Martin and Holdich 1986			12174			12174		12174			Crangonyx		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (UK)
	Amphipod Hyalella azteca		F M T		105		613		048		gt		5997		Wang et al 2016 Wang et al 2017		gt		28002		gt		28002		28002		gt		Hyalella		Invert		Author reported
	Stonefly Acroneuria sp		S M T		474		746		11		gt		22600		Call et al 1984		gt		20498		gt		20498		20498		gt		Acroneuria		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Midge Chironomus plumosus		S U T		80		70		16			30000		Fargasova 2001			25361			25361		25361			Chironomus		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (Slovakia)
	Midge Paratanytarsus dissimilis		S M T		1743		728		28		gt		77700		Lamb and Bailey 1981 1983		gt		62318		gt		62318		62318		gt		Paratanytarsus		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Liberty Lake Washington
	Rainbow trout Oncorhynchus mykiss		F M T		274		758		05		gt		9840		Gundersen et al 1994		gt		5745			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		452		762		05		gt		8070		Gundersen et al 1994		gt		5212			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		895		758		05		gt		8160		Gundersen et al 1994		gt		6807			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		1304		758		05		gt		8200		Gundersen et al 1994		gt		7662			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		232		825		05			6170		Gundersen et al 1994			1190			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		350		826		05			6170		Gundersen et al 1994			1523			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		836		829		05			7670		Gundersen et al 1994			3205			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		1158		830		05			6930		Gundersen et al 1994			3554			3661		3661			Oncorhynchus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Atlantic salmon Salmo salar		S U T		68		55		05			584		Hamilton and Haines 1995			21042			-				Salmo			Value is from 2007 FW Cu AWQC Appendix C recommendation		Soft recon
	Atlantic salmon Salmo salar		S U T		68		65		05			599		Hamilton and Haines 1995			2430			7151		7151			Salmo		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Soft recon
	Brook trout Salvelinus fontinalis		S U T		40		56		16			6530		Tandjung 1982			31767			-				Salvelinus			Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Brook trout Salvelinus fontinalis		S U T		18		56		16			3400		Tandjung 1982			28391			-				Salvelinus			Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Brook trout Salvelinus fontinalis		S U T		2		56		16			370		Tandjung 1982			13663			23097		23097			Salvelinus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Green sunfish Lepomis cyanellus		S M T		474		755		11		gt		50000		Call et al 1984		gt		24028		gt		24028		24028		gt		Lepomis		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Rio Grande silvery minnow Hybognathus amarus		R M T		140		81		05		gt		59100		Buhl 2002		gt		39414		gt		39414		39414		gt		Hybognathus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon water
	Fathead minnow Pimephales promelas		S M T		474		761		11		gt		48200		Call et al 1984		gt		21522			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Fathead minnow Pimephales promelas		S M T		474		805		11		gt		49800		Call et al 1984		gt		12972			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Fathead minnow Pimephales promelas		R M T		26		78		05			1160		ENSR 1992c			477			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		46		76		05			8180		ENSR 1992c			5446			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		96		81		05			20300		ENSR 1992c			10966			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		194		81		05			44800		ENSR 1992c			35847			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		140		81		05		gt		59100		Buhl 2002		gt		39414			10168		10168			Pimephales		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon water
	Smallmouth bass Micropterus dolomieui		S M T		1245		505		16			130		Kane and Rabeni 1987			3929			3929		3929			Micropterus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Green tree frog Hyla cinerea		R M T		455		549		05		gt		4052		Jung and Jagoe 1995		gt		20016		gt		20016		20016		gt		Hyla		Amphibian		Value is from 2007 FW Cu AWQC Appendix C recommendation		artificial soft recon

	Normalization Chemistry
	pH		7					FAV		2741
	Hardness		100					CMC		1400
	DOC		1					CCC		390
	Acute Ranked GMAV											Chronic Ranked GMCV
	Rank			GMAV		Genus								Rank			GMCV		Genus
	18		gt		62318		Paratanytarsus		Invert							12			17098		Aeolosoma		Invert
	17		gt		39414		Hybognathus		Fish							11		gt		9746		Rana		Amphib - Other Data
	16			35462		Physa		Mollusk							10			5908		Chironomus		Invert
	15		gt		29834		Lampsilis		Mollusk							9			4877		Lymnaea		Mollusk
	14		gt		28002		Hyalella		Invert							8			2555		Brachionus		Invert
	13			25361		Chironomus		Invert							7			2488		Pimephales		Fish
	12		gt		24028		Lepomis		Fish							6			1713		Hyalella		Invert
	11			23097		Salvelinus		Fish							5			1182		Ceriodaphnia		Invert
	10		gt		20498		Acroneuria		Invert							4			1102		Danio		Fish
	9		gt		20016		Hyla		Amphibian							3			1042		Lampsilis		Mollusk
	8			12174		Crangonyx		Invert							2			591		Salvelinus		Fish
	7			10216		Stenocypris		Invert							1			509		Salmo		Fish
	6			10168		Pimephales		Fish
	5			7328		Ceriodaphnia		Invert
	4			7151		Salmo		Fish
	3			3929		Micropterus		Fish
	2			3661		Oncorhynchus		Fish
	1			3332		Daphnia		Invert
		N		Rank		Genus		GMAV		ln(GMAV)		ln(GMAV)2		P=R(N+1)		sqrt(P)				N		Rank		Genus		GMCV		ln(GMCV)		ln(GMCV)2		P=R(N+1)		sqrt(P)
		18		4		Salmo		7151		887		7876		0211		0459				12		4		Danio		1102		700		4907		0308		0555
			3		Micropterus		3929		828		6849		0158		0397					3		Lampsilis		1042		695		4829		0231		0480
			2		Oncorhynchus		3661		821		6733		0105		0324					2		Salvelinus		591		638		4074		0154		0392
			1		Daphnia		3332		811		6579		0053		0229					1		Salmo		509		623		3883		0077		0277
			Sum				3347		2804		0526		141					Sum				2657		1769		0769		170
			S2 =		1223										S2 =		1080
			L =		7134										L =		5242
			A =		7916										A =		5976
			FAV =		2741										FCV =		3940
			CMC =		1371

				All concentrations reported are microgL total Aluminum
				lt-----Click Button after entering data
	ENTER DATA HERE									ERRORNUM		ACUTE														CHRONIC
										Rank 1				Rank 2				Rank 3				Rank 4					Rank 1				Rank 2				Rank 3				Rank 4
	SiteName		DOC(mgL)		Hardness(mgL as CaCO3)		pH			FAV		CMC		CCC			Genus		GMAV			Genus		GMAV			Genus		GMAV			Genus		GMAV				Genus		GMCV			Genus		GMCV			Genus		GMCV			Genus		GMCV
	Site 1		50		25		9		75428737034162		35229324666008		1800		770			Daphnia			33452		Ceriodaphnia			73576		Stenocypris			102576		Crangonyx			122230			Lampsilis			10463		Ceriodaphnia			11865		Hyalella			17200		Brachionus			25648
	Site 2		50		50		9		75428737034162		31204991509063		1600		680			Daphnia			29631		Ceriodaphnia			65171		Stenocypris			90859		Crangonyx			108267			Lampsilis			9268		Ceriodaphnia			10510		Hyalella			15236		Brachionus			22718
	Site 3		50		75		9		75428737034162		29067527928199		1500		640			Daphnia			27601		Ceriodaphnia			60707		Stenocypris			84635		Crangonyx			100851			Lampsilis			8633		Ceriodaphnia			9790		Hyalella			14192		Brachionus			21162
	Site 4		50		100		9		75428737034162		27640367912592		1400		600			Daphnia			26246		Ceriodaphnia			57726		Stenocypris			80480		Crangonyx			95899			Lampsilis			8209		Ceriodaphnia			9309		Hyalella			13495		Brachionus			20123
	Site 5		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 6		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 7		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 8		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 9		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 10		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 11		50		25		85		75428737034162		63245275664384		3200		1400			Daphnia			60055		Ceriodaphnia			132086		Stenocypris			184149		Crangonyx			219432			Lampsilis			18783		Ceriodaphnia			21301		Hyalella			30879		Brachionus			46045
	Site 12		50		50		85		75428737034162		61388044609926		3100		1300			Daphnia			58291		Ceriodaphnia			128208		Stenocypris			178742		Crangonyx			212988			Lampsilis			18232		Ceriodaphnia			20676		Hyalella			29972		Brachionus			44693
	Site 13		50		75		85		75428737034162		60327020429132		3000		1300			Daphnia			57284		Ceriodaphnia			125992		Stenocypris			175652		Crangonyx			209307			Lampsilis			17917		Ceriodaphnia			20318		Hyalella			29454		Brachionus			43920
	Site 14		50		100		85		75428737034162		59585352130146		3000		1300			Daphnia			56580		Ceriodaphnia			124443		Stenocypris			173493		Crangonyx			206734			Lampsilis			17696		Ceriodaphnia			20068		Hyalella			29092		Salmo			41264
	Site 15		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 16		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 17		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 18		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 19		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 20		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370

Aluminum Criteria Calculatorbull The relative ranking of genera in the sensitivity

distribution to which the toxicity data are normalized will change based on the set of water chemistry conditions (ie pH hardness and DOC)

bull To make calculating aluminum criteria easier EPA developed an Aluminum Criteria Calculatorndash Automated so that user only needs to enter water chemistry

conditions of interestndash Allows quick calculations for a variety of conditionsndash Limits calculations to the recommended conditions for which

the MLR models were developed were specified for hardness and DOC

bull No extrapolation beyond hardness and DOC empirical data bounds

23

Aluminum Criteria Calculator SD Example

24

pH=7 Hardness=100 DOC=1 pH=6 Hardness=25 DOC=1

Chronic Ranked GMCVRank GMCV Genus

12 17098 Aeolosoma Invert11 gt 9746 Rana Amphib - Other Data10 5908 Chironomus Invert

9 4877 Lymnaea Mollusk8 2555 Brachionus Invert7 2488 Pimephales Fish6 1713 Hyalella Invert5 1182 Ceriodaphnia Invert4 1102 Danio Fish3 1042 Lampsilis Mollusk2 591 Salvelinus Fish1 509 Salmo Fish

FCV = 3940 microgL total aluminum

Chronic Ranked GMCVRank GMCV Genus

12 gt 2131 Rana Amphib - Other Data11 1710 Aeolosoma Invert10 591 Chironomus Invert

9 544 Pimephales Fish8 488 Lymnaea Mollusk7 255 Brachionus Invert6 241 Danio Fish5 171 Hyalella Invert4 129 Salvelinus Fish3 118 Ceriodaphnia Invert2 111 Salmo Fish1 104 Lampsilis Mollusk

FCV = 987 microgL total aluminum

Multiple Scenarios

Run Scenarios

Summary Sheet

Acute Dataset

Chronic Dataset

Multiple Scenarios

Run Scenarios

Summary Sheet

Acute Dataset

Chronic Dataset

Boundaries of the Criteria Outputsbull MLR models were developed using data that

encompassndash DOC range of 008-5 mgLndash Hardness range of 98-127 mgL (as CaCO3)ndash pH range of 6-81

bull MLR criteria outputs are bounded atndash Maximum of 150 mgL total hardness (as CaCO3)

ndash DOC of 50 mgLndash pH range of the model is 50 to 90

25

Aluminum Criteria Look-Up Tables

26

bull EPA also provided criteria look-up tables in Appendix K

Har

dnes

s

CCC(microgL total aluminum)

(DOC=05 mgL)

pH 50 pH 55 pH 60 pH 65 pH 70 pH 75 pH 80 pH 85 pH 90

25 23 a 14 b 69 c 160 f 350 f 620 i 540 b 410 a 230 a

50 43 a 24 b 100 d 170 f 310 f 630 f 670 j 400 a 200 a

75 61 a 33 b 130 e 180 f 290 f 530 f 730 i 390 a 190 a

100 78 a 41 b 150 e 190 f 280 g 460 f 770 c 390 a 180 a

150 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

200 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

250 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

300 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

350 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

400 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

Aluminum Criteria Look-Up Tables

27

bull EPA also provided criteria look-up tables in Appendix K

Har

dnes

s

CCC(microgL total aluminum)

(DOC=10 mgL)

pH 50 pH 55 pH 60 pH 65 pH 70 pH 75 pH 80 pH 85 pH 90

25 33 a 21 b 99 c 220 f 490 f 900 i 780 b 590 a 330 a

50 61 a 35 b 150 e 250 f 440 f 890 f 960 j 580 a 290 a

75 88 a 47 b 180 e 260 f 410 f 740 f 1100 i 570 a 270 a

100 11 a 59 b 210 e 270 f 390 g 650 f 1100 c 560 b 260 a

150 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

200 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

250 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

300 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

350 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

400 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

Aluminum Criteria Look-Up Tables

28

bull EPA also provided criteria look-up tables in Appendix K

Har

dnes

s

CCC(microgL total aluminum)

(DOC=25 mgL)

pH 50 pH 55 pH 60 pH 65 pH 70 pH 75 pH 80 pH 85 pH 90

25 54 a 34 b 160 c 350 f 770 f 1500 i 1300 b 960 a 540 a

50 99 a 57 b 230 e 390 f 690 f 1400 f 1600 j 930 a 470 a

75 14 a 77 b 290 e 410 f 640 f 1200 f 1700 i 920 a 440 a

100 18 a 96 b 330 e 420 f 620 g 1000 f 1800 d 920 b 420 a

150 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

200 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

250 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

300 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

350 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

400 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

Aluminum Criteria Look-Up Tables

29

bull EPA also provided criteria look-up tables in Appendix K

Har

dnes

s

CCC(microgL total aluminum)

(DOC=50 mgL)

pH 50 pH 55 pH 60 pH 65 pH 70 pH 75 pH 80 pH 85 pH 90

25 77 a 49 b 230 c 500 f 1100 f 2100 c 1900 b 1400 a 770 a

50 14 a 82 b 330 e 540 f 970 f 2000 f 2200 j 1300 a 680 a

75 20 a 110 b 410 e 570 f 900 f 1600 f 2500 i 1300 a 640 a

100 26 a 140 b 460 f 590 f 880 g 1400 f 2500 d 1300 b 600 a

150 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

200 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

250 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

300 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

350 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

400 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

Status

30

bull Draft Document Released to the Federal Register on 72817ndash Public comment period of 60 days ndash Due Date for Comments is 92617

bull Revisions will be made considering public commentsbull Subsequent EPA intra-agency review of final criteria

document will then be conducted bull Publication of final criteria document planned for Late Fall

2017

Federal Register Webpagehttpswwwfederalregistergovdocuments201

707282017-15968request-for-scientific-views-draft-updated-aquatic-life-ambient-water-quality-criteria-for-aluminum

Aluminum Criteria Webpagehttpswwwepagovwqc2017-draft-aquatic-life-

criteria-aluminum-freshwater-documents

31

	Appendix C Acceptable Chronic Toxicity Data of Aluminum to Freshwater Aquatic Animals
									MLR equation
	Species		Hardness (mgL as CaCO3)		pH		DOC(mgL)		EC20 Endpoint			EC20(microgL)			Normalized Conc- EC20(microgL)			SMCV-EC20(microgL)		GMCV-EC20(microgL)			Genus			DOC Notes		Dilution water
	Oligochaete Aeolosoma sp		48		6		025		Reproduction (population size)			1235			1710			1710		1710			Aeolosoma		Invert		Author reported half the detection limit
	Rotifer Brachionus calyciflorus		100		62		025		Reproduction (population size)			431			255			255		255			Brachionus		Invert		Author reported half the detection limit
	Great pond snail Lymnaea stagnalis		117		61		025		Biomass			746			488			488		488			Lymnaea		Mollusk		Author reported half the detection limit
	Fatmucket Lampsilis siliquoidea		1055		604		040		Biomass			169			104			104		104			Lampsilis		Mollusk		Author reported
	Cladoceran Ceriodaphnia dubia		50		715		11		Reproduction - youngstarting adult			1780			190						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		26		73		05		Reproduction - youngfemale			1557			272						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		46		75		05		Reproduction - youngfemale			8087			110						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		96		79		05		Reproduction - youngfemale			6519			80						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		194		81		05		Reproduction - youngfemale			6836			89						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		255		703		05		Reproduction (youngfemale)			250			56						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		122		713		05		Reproduction (youngfemale)			860			106						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		25		798		05		Reproduction (youngfemale)			700			101						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		61		803		05		Reproduction (youngfemale)			1010			136						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		122		810		05		Reproduction (youngfemale)			870			116						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		255		633		05		Reproduction (youngfemale)			260			176						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		121		636		05		Reproduction (youngfemale)			390			110			118		118			Ceriodaphnia		Invert		Author reported
	Amphipod Hyalella azteca		95		61		051		Biomass (28 d)			1993			101						Hyalella			Author reported
	Amphipod Hyalella azteca		106		604		033		Biomass (28 d)			425			289			171		171			Hyalella		Invert		Author reported
	Midge Chironomus riparius		118		558		18		Adult midge emergence			2955			119						Chironomus			Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Midge Chironomus riparius		119		505		18		Adult midge emergence			8442			2258						Chironomus			Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Midge Chironomus riparius		91		66		051		Reproduction ( of eggscase)			3387			766			591		591			Chironomus		Invert		Author reported
	Atlantic salmon Salmo salar		127		57		18		Biomass			6156			1112			1112		1112			Salmo		Fish		Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Brook trout Salvelinus fontinalis		123		655		19		Biomass			1644			59						Salvelinus			Author reported		wellRO mix
	Brook trout Salvelinus fontinalis		128		565		18		Biomass			1435			283			1293		1293			Salvelinus		Fish		Author reported		wellRO mix
	Fathead minnow Pimephales promelas		220		770		16		Biomass			6194			781						Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		well water
	Fathead minnow Pimephales promelas		96		620		025		Fry survival			4286			379			544		544			Pimephales		Fish		Author reported half the detection limit
	Zebrafish Danio rerio		83		61		025		Biomass			2344			241			241		241			Danio		Fish		Author reported half the detection limit
	Wood frog Rana sylvatica		115		469		16		MATC-survival		gt		2000		gt		2131		gt		2131		2131		gt		Rana		Amphib - Other Data		Value is from 2007 FW Cu AWQC Appendix C recommendation		aged tap water

	Appendix A Acceptable Acute Toxicity Data of Aluminum to Freshwater Aquatic Animals
	Species		Methoda		Hardness (mgL as CaCO3)		pH		DOC(mgL)			LC50 or EC50 Conc (microgL)		Reference			Normalized Conc - LC50(microgL)			Species Mean Acute Value(microgL)		Genus Mean Acute Value(microgL)			Genus			DOC Note		Dilution water
	Snail Physa sp		S M T		474		659		11		gt		23400		Call 1984 Call et al 1984		gt		4843			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		755		11			30600		Call 1984 Call et al 1984			2699			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		817		11		gt		24700		Call 1984 Call et al 1984		gt		2404			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		746		11			55500		Call 1984 Call et al 1984			5034			3546		3546			Physa		Mollusk		Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Fatmucket Lampsilis siliquoidea		F M T		1060		612		048		gt		6302		Wang et al 2016 Wang et al 2017		gt		2983		gt		2983		2983		gt		Lampsilis		Mollusk		Author reported
	Cladoceran Ceriodaphnia dubia		S M A		500		742		11			1900		McCauley et al 1986			173			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		S M A		505		786		11			1500		McCauley et al 1986			129			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		UW-S Lake Superior lab water
	Cladoceran Ceriodaphnia dubia		S M A		500		813		11			2560		McCauley et al 1986			243			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		R M T		26		75		05			720		ENSR 1992d			111			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		R M T		46		76		05			1880		ENSR 1992d			250			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		R M T		96		78		05			2450		ENSR 1992d			289			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		S U NR		90		715		05			3727		Fort and Stover 1995			500			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U NR		90		715		05			5673		Fort and Stover 1995			761			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U NR		89		76		05			2880		Soucek et al 2001			336			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		601		05			7112		European Al Association 2009			169			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		605		2			6865		European Al Association 2009			709			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		609		4			15581		European Al Association 2009			1007			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		601		05			681		European Al Association 2009			162			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		603		05			1630		European Al Association 2009			367			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		597		05			1785		European Al Association 2009			473			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		592		05			1410		European Al Association 2009			429			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		699		05		gt		1300		European Al Association 2009		gt		419			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		785		05		gt		5000		European Al Association 2009		gt		796			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		68		2		gt		10000		European Al Association 2009		gt		2076			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		782		2		gt		15000		European Al Association 2009		gt		1163			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		677		4		gt		10000		European Al Association 2009		gt		1516			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		766		4		gt		15000		European Al Association 2009		gt		861			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		79		05		gt		2000		European Al Association 2009		gt		315			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		789		05		gt		2000		European Al Association 2009		gt		316			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		604		05			1108		European Al Association 2009			85			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		598		2			11371		European Al Association 2009			482			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		573		4			80467		European Al Association 2009			4357			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		671		05		gt		10000		European Al Association 2009		gt		2379			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		783		05		gt		5000		European Al Association 2009		gt		634			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		679		2		gt		10000		European Al Association 2009		gt		1039			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		767		2		gt		15000		European Al Association 2009		gt		908			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		668		4		gt		15000		European Al Association 2009		gt		1246			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		762		4		gt		15000		European Al Association 2009		gt		633			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		606		2			33868		European Al Association 2009			792			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		56		4			104842		European Al Association 2009			4880			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		693		05		gt		5000		European Al Association 2009		gt		713			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		120		788		05		gt		5000		European Al Association 2009		gt		590			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		120		676		2		gt		15000		European Al Association 2009		gt		1212			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		771		2		gt		15000		European Al Association 2009		gt		810			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		66		4		gt		15000		European Al Association 2009		gt		1014			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		76		4		gt		15000		European Al Association 2009		gt		555			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			11971		European Al Association 2010			270			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			27478		European Al Association 2010			619			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			11998		European Al Association 2010			270			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		607		05			92495		European Al Association 2010			188			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		609		05			31337		European Al Association 2010			604			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		61		05			33235		European Al Association 2010			624			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		708		05		gt		8864		European Al Association 2010		gt		253			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		779		05		gt		42783		European Al Association 2010		gt		693			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		753		05			13204		European Al Association 2010			24			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		600		601		05			46326		European Al Association 2010			380			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		600		599		05		gt		8590		European Al Association 2010		gt		737			509				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia reticulata		F M T		451		60		11			304		Shephard 1983			201			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia reticulata		F M T		40		55		11			362		Shephard 1983			5531			1054		733			Ceriodaphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake SuperiorDI Mix
	Cladoceran Daphnia magna		S U NR		453		774		11			3900		Biesinger and Christensen 1972			338			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Daphnia magna		S M T		220		76		16			38200		Kimball 1978			2030			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Well water
	Cladoceran Daphnia magna		S U T		451		725		11			2800		Shephard 1983			287			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Daphnia magna		S U T		168		599		05			5000		European Al Association 2009		gt		227			-				Daphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Daphnia magna		S U T		168		792		05			7950		European Al Association 2009			92			333		333			Daphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Ostracod Stenocypris major		R M T		1563		651		16			310196		Shuhaimi-Othman et al 2011a			1022			1022		1022			Stenocypris		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (Malaysia)
	Amphipod Crangonyx pseudogracilis		R U T		50		675		16			9190		Martin and Holdich 1986			1217			1217		1217			Crangonyx		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (UK)
	Amphipod Hyalella azteca		F M T		105		613		048		gt		5997		Wang et al 2016 Wang et al 2017		gt		2800		gt		2800		2800		gt		Hyalella		Invert		Author reported
	Stonefly Acroneuria sp		S M T		474		746		11		gt		22600		Call et al 1984		gt		2050		gt		2050		2050		gt		Acroneuria		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Midge Chironomus plumosus		S U T		80		70		16			30000		Fargasova 2001			2536			2536		2536			Chironomus		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (Slovakia)
	Midge Paratanytarsus dissimilis		S M T		1743		728		28		gt		77700		Lamb and Bailey 1981 1983		gt		6232		gt		6232		6232		gt		Paratanytarsus		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Liberty Lake Washington
	Rainbow trout Oncorhynchus mykiss		F M T		274		758		05		gt		9840		Gundersen et al 1994		gt		1256			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		452		762		05		gt		8070		Gundersen et al 1994		gt		1140			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		895		758		05		gt		8160		Gundersen et al 1994		gt		1489			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		1304		758		05		gt		8200		Gundersen et al 1994		gt		1676			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		232		825		05			6170		Gundersen et al 1994			260			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		350		826		05			6170		Gundersen et al 1994			333			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		836		829		05			7670		Gundersen et al 1994			701			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		1158		830		05			6930		Gundersen et al 1994			777			801		801			Oncorhynchus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Atlantic salmon Salmo salar		S U T		68		55		05			584		Hamilton and Haines 1995			4602			-				Salmo			Value is from 2007 FW Cu AWQC Appendix C recommendation		Soft recon
	Atlantic salmon Salmo salar		S U T		68		65		05			599		Hamilton and Haines 1995			531			1564		1564			Salmo		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Soft recon
	Brook trout Salvelinus fontinalis		S U T		40		56		16			6530		Tandjung 1982			6947			-				Salvelinus			Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Brook trout Salvelinus fontinalis		S U T		18		56		16			3400		Tandjung 1982			6209			-				Salvelinus			Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Brook trout Salvelinus fontinalis		S U T		2		56		16			370		Tandjung 1982			2988			5051		5051			Salvelinus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Green sunfish Lepomis cyanellus		S M T		474		755		11		gt		50000		Call et al 1984		gt		5255		gt		5255		5255		gt		Lepomis		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Rio Grande silvery minnow Hybognathus amarus		R M T		140		81		05		gt		59100		Buhl 2002		gt		8620		gt		8620		8620		gt		Hybognathus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon water
	Fathead minnow Pimephales promelas		S M T		474		761		11		gt		48200		Call et al 1984		gt		4707			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Fathead minnow Pimephales promelas		S M T		474		805		11		gt		49800		Call et al 1984		gt		2837			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Fathead minnow Pimephales promelas		R M T		26		78		05			1160		ENSR 1992c			104			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		46		76		05			8180		ENSR 1992c			1191			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		96		81		05			20300		ENSR 1992c			2398			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		194		81		05			44800		ENSR 1992c			7839			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		140		81		05		gt		59100		Buhl 2002		gt		8620			2224		2224			Pimephales		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon water
	Smallmouth bass Micropterus dolomieui		S M T		1245		505		16			130		Kane and Rabeni 1987			859			859		859			Micropterus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Green tree frog Hyla cinerea		R M T		455		549		05		gt		4052		Jung and Jagoe 1995		gt		4377		gt		4377		4377		gt		Hyla		Amphibian		Value is from 2007 FW Cu AWQC Appendix C recommendation		artificial soft recon

	Normalization Chemistry
	pH		6					FAV		360
	Hardness		25					CMC		180
	DOC		1					CCC		99
	Acute Ranked GMAV											Chronic Ranked GMCV
	Rank			GMAV		Genus								Rank			GMCV		Genus
	18		gt		8620		Hybognathus		Fish							12		gt		2131		Rana		Amphib - Other Data
	17		gt		6232		Paratanytarsus		Invert							11			1710		Aeolosoma		Invert
	16		gt		5255		Lepomis		Fish							10			591		Chironomus		Invert
	15			5051		Salvelinus		Fish							9			544		Pimephales		Fish
	14		gt		4377		Hyla		Amphibian							8			488		Lymnaea		Mollusk
	13			3546		Physa		Mollusk							7			255		Brachionus		Invert
	12		gt		2983		Lampsilis		Mollusk							6			241		Danio		Fish
	11		gt		2800		Hyalella		Invert							5			171		Hyalella		Invert
	10			2536		Chironomus		Invert							4			129		Salvelinus		Fish
	9			2224		Pimephales		Fish							3			118		Ceriodaphnia		Invert
	8		gt		2050		Acroneuria		Invert							2			111		Salmo		Fish
	7			1564		Salmo		Fish							1			104		Lampsilis		Mollusk
	6			1217		Crangonyx		Invert
	5			1022		Stenocypris		Invert
	4			859		Micropterus		Fish
	3			801		Oncorhynchus		Fish
	2			733		Ceriodaphnia		Invert
	1			333		Daphnia		Invert
		N		Rank		Genus		GMAV		ln(GMAV)		ln(GMAV)2		P=R(N+1)		sqrt(P)				N		Rank		Genus		GMCV		ln(GMCV)		ln(GMCV)2		P=R(N+1)		sqrt(P)
		18		4		Micropterus		859		676		4564		0211		0459				12		4		Salvelinus		129		486		2364		0308		0555
			3		Oncorhynchus		801		669		4469		0158		0397					3		Ceriodaphnia		118		477		2277		0231		0480
			2		Ceriodaphnia		733		660		4352		0105		0324					2		Salmo		111		471		2220		0154		0392
			1		Daphnia		333		581		3374		0053		0229					1		Lampsilis		104		465		2159		0077		0277
			Sum				2585		1676		0526		141					Sum				1899		902		0769		170
			S2 =		1987										S2 =		059
			L =		4890										L =		4420
			A =		5887										A =		4592
			FAV =		360										FCV =		987
			CMC =		180

				All concentrations reported are microgL total Aluminum
				lt-----Click Button after entering data
	ENTER DATA HERE									ERRORNUM		ACUTE														CHRONIC
										Rank 1				Rank 2				Rank 3				Rank 4					Rank 1				Rank 2				Rank 3				Rank 4
	SiteName		DOC(mgL)		Hardness(mgL as CaCO3)		pH			FAV		CMC		CCC			Genus		GMAV			Genus		GMAV			Genus		GMAV			Genus		GMAV				Genus		GMCV			Genus		GMCV			Genus		GMCV			Genus		GMCV
	Site 1		50		25		9		75428737034162		35229324666008		1800		770			Daphnia			33452		Ceriodaphnia			73576		Stenocypris			102576		Crangonyx			122230			Lampsilis			10463		Ceriodaphnia			11865		Hyalella			17200		Brachionus			25648
	Site 2		50		50		9		75428737034162		31204991509063		1600		680			Daphnia			29631		Ceriodaphnia			65171		Stenocypris			90859		Crangonyx			108267			Lampsilis			9268		Ceriodaphnia			10510		Hyalella			15236		Brachionus			22718
	Site 3		50		75		9		75428737034162		29067527928199		1500		640			Daphnia			27601		Ceriodaphnia			60707		Stenocypris			84635		Crangonyx			100851			Lampsilis			8633		Ceriodaphnia			9790		Hyalella			14192		Brachionus			21162
	Site 4		50		100		9		75428737034162		27640367912592		1400		600			Daphnia			26246		Ceriodaphnia			57726		Stenocypris			80480		Crangonyx			95899			Lampsilis			8209		Ceriodaphnia			9309		Hyalella			13495		Brachionus			20123
	Site 5		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 6		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 7		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 8		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 9		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 10		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 11		50		25		85		75428737034162		63245275664384		3200		1400			Daphnia			60055		Ceriodaphnia			132086		Stenocypris			184149		Crangonyx			219432			Lampsilis			18783		Ceriodaphnia			21301		Hyalella			30879		Brachionus			46045
	Site 12		50		50		85		75428737034162		61388044609926		3100		1300			Daphnia			58291		Ceriodaphnia			128208		Stenocypris			178742		Crangonyx			212988			Lampsilis			18232		Ceriodaphnia			20676		Hyalella			29972		Brachionus			44693
	Site 13		50		75		85		75428737034162		60327020429132		3000		1300			Daphnia			57284		Ceriodaphnia			125992		Stenocypris			175652		Crangonyx			209307			Lampsilis			17917		Ceriodaphnia			20318		Hyalella			29454		Brachionus			43920
	Site 14		50		100		85		75428737034162		59585352130146		3000		1300			Daphnia			56580		Ceriodaphnia			124443		Stenocypris			173493		Crangonyx			206734			Lampsilis			17696		Ceriodaphnia			20068		Hyalella			29092		Salmo			41264
	Site 15		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 16		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 17		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 18		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 19		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 20		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370

	Appendix C Acceptable Chronic Toxicity Data of Aluminum to Freshwater Aquatic Animals
									MLR equation
	Species		Hardness (mgL as CaCO3)		pH		DOC(mgL)		EC20 Endpoint			EC20(microgL)			Normalized Conc- EC20(microgL)			SMCV-EC20(microgL)		GMCV-EC20(microgL)			Genus			DOC Notes		Dilution water
	Oligochaete Aeolosoma sp		48		6		025		Reproduction (population size)			1235			17098			17098		17098			Aeolosoma		Invert		Author reported half the detection limit
	Rotifer Brachionus calyciflorus		100		62		025		Reproduction (population size)			431			2555			2555		2555			Brachionus		Invert		Author reported half the detection limit
	Great pond snail Lymnaea stagnalis		117		61		025		Biomass			746			4877			4877		4877			Lymnaea		Mollusk		Author reported half the detection limit
	Fatmucket Lampsilis siliquoidea		1055		604		040		Biomass			169			1042			1042		1042			Lampsilis		Mollusk		Author reported
	Cladoceran Ceriodaphnia dubia		50		715		11		Reproduction - youngstarting adult			1780			1898						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		26		73		05		Reproduction - youngfemale			1557			2718						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		46		75		05		Reproduction - youngfemale			8087			1102						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		96		79		05		Reproduction - youngfemale			6519			796						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		194		81		05		Reproduction - youngfemale			6836			888						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		255		703		05		Reproduction (youngfemale)			250			563						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		122		713		05		Reproduction (youngfemale)			860			1061						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		25		798		05		Reproduction (youngfemale)			700			1006						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		61		803		05		Reproduction (youngfemale)			1010			1363						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		122		810		05		Reproduction (youngfemale)			870			1164						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		255		633		05		Reproduction (youngfemale)			260			1758						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		121		636		05		Reproduction (youngfemale)			390			1101			1182		1182			Ceriodaphnia		Invert		Author reported
	Amphipod Hyalella azteca		95		61		051		Biomass (28 d)			1993			1014						Hyalella			Author reported
	Amphipod Hyalella azteca		106		604		033		Biomass (28 d)			425			2895			1713		1713			Hyalella		Invert		Author reported
	Midge Chironomus riparius		118		558		18		Adult midge emergence			2955			1192						Chironomus			Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Midge Chironomus riparius		119		505		18		Adult midge emergence			8442			22578						Chironomus			Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Midge Chironomus riparius		91		66		051		Reproduction ( of eggscase)			3387			7664			5908		5908			Chironomus		Invert		Author reported
	Atlantic salmon Salmo salar		127		57		18		Biomass			6156			5085			5085		5085			Salmo		Fish		Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Brook trout Salvelinus fontinalis		123		655		19		Biomass			1644			270						Salvelinus			Author reported		wellRO mix
	Brook trout Salvelinus fontinalis		128		565		18		Biomass			1435			1295			5914		5914			Salvelinus		Fish		Author reported		wellRO mix
	Fathead minnow Pimephales promelas		220		770		16		Biomass			6194			3569						Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		well water
	Fathead minnow Pimephales promelas		96		620		025		Fry survival			4286			1734			2488		2488			Pimephales		Fish		Author reported half the detection limit
	Zebrafish Danio rerio		83		61		025		Biomass			2344			1102			1102		1102			Danio		Fish		Author reported half the detection limit
	Wood frog Rana sylvatica		115		469		16		MATC-survival		gt		2000		gt		9746		gt		9746		9746		gt		Rana		Amphib - Other Data		Value is from 2007 FW Cu AWQC Appendix C recommendation		aged tap water

	Appendix A Acceptable Acute Toxicity Data of Aluminum to Freshwater Aquatic Animals
	Species		Methoda		Hardness (mgL as CaCO3)		pH		DOC(mgL)			LC50 or EC50 Conc (microgL)		Reference			Normalized Conc - LC50(microgL)			Species Mean Acute Value(microgL)		Genus Mean Acute Value(microgL)			Genus			DOC Note		Dilution water
	Snail Physa sp		S M T		474		659		11		gt		23400		Call 1984 Call et al 1984		gt		48425			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		755		11			30600		Call 1984 Call et al 1984			26986			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		817		11		gt		24700		Call 1984 Call et al 1984		gt		24040			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		746		11			55500		Call 1984 Call et al 1984			50338			35462		35462			Physa		Mollusk		Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Fatmucket Lampsilis siliquoidea		F M T		1060		612		048		gt		6302		Wang et al 2016 Wang et al 2017		gt		29834		gt		29834		29834		gt		Lampsilis		Mollusk		Author reported
	Cladoceran Ceriodaphnia dubia		S M A		500		742		11			1900		McCauley et al 1986			1728			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		S M A		505		786		11			1500		McCauley et al 1986			1294			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		UW-S Lake Superior lab water
	Cladoceran Ceriodaphnia dubia		S M A		500		813		11			2560		McCauley et al 1986			2431			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		R M T		26		75		05			720		ENSR 1992d			1113			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		R M T		46		76		05			1880		ENSR 1992d			2495			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		R M T		96		78		05			2450		ENSR 1992d			2894			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		S U NR		90		715		05			3727		Fort and Stover 1995			5002			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U NR		90		715		05			5673		Fort and Stover 1995			7613			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U NR		89		76		05			2880		Soucek et al 2001			3362			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		601		05			7112		European Al Association 2009			1691			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		605		2			6865		European Al Association 2009			7088			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		609		4			15581		European Al Association 2009			10072			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		601		05			681		European Al Association 2009			1620			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		603		05			1630		European Al Association 2009			3674			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		597		05			1785		European Al Association 2009			4732			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		592		05			1410		European Al Association 2009			4295			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		699		05		gt		1300		European Al Association 2009		gt		4189			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		785		05		gt		5000		European Al Association 2009		gt		7960			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		68		2		gt		10000		European Al Association 2009		gt		20755			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		782		2		gt		15000		European Al Association 2009		gt		11626			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		677		4		gt		10000		European Al Association 2009		gt		15161			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		766		4		gt		15000		European Al Association 2009		gt		8608			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		79		05		gt		2000		European Al Association 2009		gt		3150			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		789		05		gt		2000		European Al Association 2009		gt		3156			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		604		05			1108		European Al Association 2009			850			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		598		2			11371		European Al Association 2009			4817			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		573		4			80467		European Al Association 2009			43570			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		671		05		gt		10000		European Al Association 2009		gt		23786			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		783		05		gt		5000		European Al Association 2009		gt		6344			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		679		2		gt		10000		European Al Association 2009		gt		10394			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		767		2		gt		15000		European Al Association 2009		gt		9082			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		668		4		gt		15000		European Al Association 2009		gt		12461			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		762		4		gt		15000		European Al Association 2009		gt		6332			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		606		2			33868		European Al Association 2009			7920			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		56		4			104842		European Al Association 2009			48800			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		693		05		gt		5000		European Al Association 2009		gt		7126			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		120		788		05		gt		5000		European Al Association 2009		gt		5898			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		120		676		2		gt		15000		European Al Association 2009		gt		12117			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		771		2		gt		15000		European Al Association 2009		gt		8096			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		66		4		gt		15000		European Al Association 2009		gt		10139			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		76		4		gt		15000		European Al Association 2009		gt		5545			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			11971		European Al Association 2010			2699			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			27478		European Al Association 2010			6194			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			11998		European Al Association 2010			2705			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		607		05			92495		European Al Association 2010			1876			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		609		05			31337		European Al Association 2010			6035			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		61		05			33235		European Al Association 2010			6238			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		708		05		gt		8864		European Al Association 2010		gt		2534			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		779		05		gt		42783		European Al Association 2010		gt		6930			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		753		05			13204		European Al Association 2010			244			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		600		601		05			46326		European Al Association 2010			3798			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		600		599		05		gt		8590		European Al Association 2010		gt		7366			5094				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia reticulata		F M T		451		60		11			304		Shephard 1983			2009			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia reticulata		F M T		40		55		11			362		Shephard 1983			55307			10542		7328			Ceriodaphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake SuperiorDI Mix
	Cladoceran Daphnia magna		S U NR		453		774		11			3900		Biesinger and Christensen 1972			3382			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Daphnia magna		S M T		220		76		16			38200		Kimball 1978			20303			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Well water
	Cladoceran Daphnia magna		S U T		451		725		11			2800		Shephard 1983			2868			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Daphnia magna		S U T		168		599		05			5000		European Al Association 2009		gt		2265			-				Daphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Daphnia magna		S U T		168		792		05			7950		European Al Association 2009			920			3332		3332			Daphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Ostracod Stenocypris major		R M T		1563		651		16			310196		Shuhaimi-Othman et al 2011a			10216			10216		10216			Stenocypris		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (Malaysia)
	Amphipod Crangonyx pseudogracilis		R U T		50		675		16			9190		Martin and Holdich 1986			12174			12174		12174			Crangonyx		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (UK)
	Amphipod Hyalella azteca		F M T		105		613		048		gt		5997		Wang et al 2016 Wang et al 2017		gt		28002		gt		28002		28002		gt		Hyalella		Invert		Author reported
	Stonefly Acroneuria sp		S M T		474		746		11		gt		22600		Call et al 1984		gt		20498		gt		20498		20498		gt		Acroneuria		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Midge Chironomus plumosus		S U T		80		70		16			30000		Fargasova 2001			25361			25361		25361			Chironomus		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (Slovakia)
	Midge Paratanytarsus dissimilis		S M T		1743		728		28		gt		77700		Lamb and Bailey 1981 1983		gt		62318		gt		62318		62318		gt		Paratanytarsus		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Liberty Lake Washington
	Rainbow trout Oncorhynchus mykiss		F M T		274		758		05		gt		9840		Gundersen et al 1994		gt		5745			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		452		762		05		gt		8070		Gundersen et al 1994		gt		5212			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		895		758		05		gt		8160		Gundersen et al 1994		gt		6807			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		1304		758		05		gt		8200		Gundersen et al 1994		gt		7662			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		232		825		05			6170		Gundersen et al 1994			1190			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		350		826		05			6170		Gundersen et al 1994			1523			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		836		829		05			7670		Gundersen et al 1994			3205			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		1158		830		05			6930		Gundersen et al 1994			3554			3661		3661			Oncorhynchus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Atlantic salmon Salmo salar		S U T		68		55		05			584		Hamilton and Haines 1995			21042			-				Salmo			Value is from 2007 FW Cu AWQC Appendix C recommendation		Soft recon
	Atlantic salmon Salmo salar		S U T		68		65		05			599		Hamilton and Haines 1995			2430			7151		7151			Salmo		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Soft recon
	Brook trout Salvelinus fontinalis		S U T		40		56		16			6530		Tandjung 1982			31767			-				Salvelinus			Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Brook trout Salvelinus fontinalis		S U T		18		56		16			3400		Tandjung 1982			28391			-				Salvelinus			Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Brook trout Salvelinus fontinalis		S U T		2		56		16			370		Tandjung 1982			13663			23097		23097			Salvelinus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Green sunfish Lepomis cyanellus		S M T		474		755		11		gt		50000		Call et al 1984		gt		24028		gt		24028		24028		gt		Lepomis		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Rio Grande silvery minnow Hybognathus amarus		R M T		140		81		05		gt		59100		Buhl 2002		gt		39414		gt		39414		39414		gt		Hybognathus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon water
	Fathead minnow Pimephales promelas		S M T		474		761		11		gt		48200		Call et al 1984		gt		21522			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Fathead minnow Pimephales promelas		S M T		474		805		11		gt		49800		Call et al 1984		gt		12972			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Fathead minnow Pimephales promelas		R M T		26		78		05			1160		ENSR 1992c			477			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		46		76		05			8180		ENSR 1992c			5446			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		96		81		05			20300		ENSR 1992c			10966			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		194		81		05			44800		ENSR 1992c			35847			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		140		81		05		gt		59100		Buhl 2002		gt		39414			10168		10168			Pimephales		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon water
	Smallmouth bass Micropterus dolomieui		S M T		1245		505		16			130		Kane and Rabeni 1987			3929			3929		3929			Micropterus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Green tree frog Hyla cinerea		R M T		455		549		05		gt		4052		Jung and Jagoe 1995		gt		20016		gt		20016		20016		gt		Hyla		Amphibian		Value is from 2007 FW Cu AWQC Appendix C recommendation		artificial soft recon

	Normalization Chemistry
	pH		7					FAV		2741
	Hardness		100					CMC		1400
	DOC		1					CCC		390
	Acute Ranked GMAV											Chronic Ranked GMCV
	Rank			GMAV		Genus								Rank			GMCV		Genus
	18		gt		62318		Paratanytarsus		Invert							12			17098		Aeolosoma		Invert
	17		gt		39414		Hybognathus		Fish							11		gt		9746		Rana		Amphib - Other Data
	16			35462		Physa		Mollusk							10			5908		Chironomus		Invert
	15		gt		29834		Lampsilis		Mollusk							9			4877		Lymnaea		Mollusk
	14		gt		28002		Hyalella		Invert							8			2555		Brachionus		Invert
	13			25361		Chironomus		Invert							7			2488		Pimephales		Fish
	12		gt		24028		Lepomis		Fish							6			1713		Hyalella		Invert
	11			23097		Salvelinus		Fish							5			1182		Ceriodaphnia		Invert
	10		gt		20498		Acroneuria		Invert							4			1102		Danio		Fish
	9		gt		20016		Hyla		Amphibian							3			1042		Lampsilis		Mollusk
	8			12174		Crangonyx		Invert							2			591		Salvelinus		Fish
	7			10216		Stenocypris		Invert							1			509		Salmo		Fish
	6			10168		Pimephales		Fish
	5			7328		Ceriodaphnia		Invert
	4			7151		Salmo		Fish
	3			3929		Micropterus		Fish
	2			3661		Oncorhynchus		Fish
	1			3332		Daphnia		Invert
		N		Rank		Genus		GMAV		ln(GMAV)		ln(GMAV)2		P=R(N+1)		sqrt(P)				N		Rank		Genus		GMCV		ln(GMCV)		ln(GMCV)2		P=R(N+1)		sqrt(P)
		18		4		Salmo		7151		887		7876		0211		0459				12		4		Danio		1102		700		4907		0308		0555
			3		Micropterus		3929		828		6849		0158		0397					3		Lampsilis		1042		695		4829		0231		0480
			2		Oncorhynchus		3661		821		6733		0105		0324					2		Salvelinus		591		638		4074		0154		0392
			1		Daphnia		3332		811		6579		0053		0229					1		Salmo		509		623		3883		0077		0277
			Sum				3347		2804		0526		141					Sum				2657		1769		0769		170
			S2 =		1223										S2 =		1080
			L =		7134										L =		5242
			A =		7916										A =		5976
			FAV =		2741										FCV =		3940
			CMC =		1371

				All concentrations reported are microgL total Aluminum
				lt-----Click Button after entering data
	ENTER DATA HERE									ERRORNUM		ACUTE														CHRONIC
										Rank 1				Rank 2				Rank 3				Rank 4					Rank 1				Rank 2				Rank 3				Rank 4
	SiteName		DOC(mgL)		Hardness(mgL as CaCO3)		pH			FAV		CMC		CCC			Genus		GMAV			Genus		GMAV			Genus		GMAV			Genus		GMAV				Genus		GMCV			Genus		GMCV			Genus		GMCV			Genus		GMCV
	Site 1		50		25		9		75428737034162		35229324666008		1800		770			Daphnia			33452		Ceriodaphnia			73576		Stenocypris			102576		Crangonyx			122230			Lampsilis			10463		Ceriodaphnia			11865		Hyalella			17200		Brachionus			25648
	Site 2		50		50		9		75428737034162		31204991509063		1600		680			Daphnia			29631		Ceriodaphnia			65171		Stenocypris			90859		Crangonyx			108267			Lampsilis			9268		Ceriodaphnia			10510		Hyalella			15236		Brachionus			22718
	Site 3		50		75		9		75428737034162		29067527928199		1500		640			Daphnia			27601		Ceriodaphnia			60707		Stenocypris			84635		Crangonyx			100851			Lampsilis			8633		Ceriodaphnia			9790		Hyalella			14192		Brachionus			21162
	Site 4		50		100		9		75428737034162		27640367912592		1400		600			Daphnia			26246		Ceriodaphnia			57726		Stenocypris			80480		Crangonyx			95899			Lampsilis			8209		Ceriodaphnia			9309		Hyalella			13495		Brachionus			20123
	Site 5		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 6		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 7		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 8		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 9		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 10		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 11		50		25		85		75428737034162		63245275664384		3200		1400			Daphnia			60055		Ceriodaphnia			132086		Stenocypris			184149		Crangonyx			219432			Lampsilis			18783		Ceriodaphnia			21301		Hyalella			30879		Brachionus			46045
	Site 12		50		50		85		75428737034162		61388044609926		3100		1300			Daphnia			58291		Ceriodaphnia			128208		Stenocypris			178742		Crangonyx			212988			Lampsilis			18232		Ceriodaphnia			20676		Hyalella			29972		Brachionus			44693
	Site 13		50		75		85		75428737034162		60327020429132		3000		1300			Daphnia			57284		Ceriodaphnia			125992		Stenocypris			175652		Crangonyx			209307			Lampsilis			17917		Ceriodaphnia			20318		Hyalella			29454		Brachionus			43920
	Site 14		50		100		85		75428737034162		59585352130146		3000		1300			Daphnia			56580		Ceriodaphnia			124443		Stenocypris			173493		Crangonyx			206734			Lampsilis			17696		Ceriodaphnia			20068		Hyalella			29092		Salmo			41264
	Site 15		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 16		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 17		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 18		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 19		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 20		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370

Aluminum Criteria Calculator SD Example

24

pH=7 Hardness=100 DOC=1 pH=6 Hardness=25 DOC=1

Chronic Ranked GMCVRank GMCV Genus

12 17098 Aeolosoma Invert11 gt 9746 Rana Amphib - Other Data10 5908 Chironomus Invert

9 4877 Lymnaea Mollusk8 2555 Brachionus Invert7 2488 Pimephales Fish6 1713 Hyalella Invert5 1182 Ceriodaphnia Invert4 1102 Danio Fish3 1042 Lampsilis Mollusk2 591 Salvelinus Fish1 509 Salmo Fish

FCV = 3940 microgL total aluminum

Chronic Ranked GMCVRank GMCV Genus

12 gt 2131 Rana Amphib - Other Data11 1710 Aeolosoma Invert10 591 Chironomus Invert

9 544 Pimephales Fish8 488 Lymnaea Mollusk7 255 Brachionus Invert6 241 Danio Fish5 171 Hyalella Invert4 129 Salvelinus Fish3 118 Ceriodaphnia Invert2 111 Salmo Fish1 104 Lampsilis Mollusk

FCV = 987 microgL total aluminum

Multiple Scenarios

Run Scenarios

Summary Sheet

Acute Dataset

Chronic Dataset

Multiple Scenarios

Run Scenarios

Summary Sheet

Acute Dataset

Chronic Dataset

Boundaries of the Criteria Outputsbull MLR models were developed using data that

encompassndash DOC range of 008-5 mgLndash Hardness range of 98-127 mgL (as CaCO3)ndash pH range of 6-81

bull MLR criteria outputs are bounded atndash Maximum of 150 mgL total hardness (as CaCO3)

ndash DOC of 50 mgLndash pH range of the model is 50 to 90

25

Aluminum Criteria Look-Up Tables

26

bull EPA also provided criteria look-up tables in Appendix K

Har

dnes

s

CCC(microgL total aluminum)

(DOC=05 mgL)

pH 50 pH 55 pH 60 pH 65 pH 70 pH 75 pH 80 pH 85 pH 90

25 23 a 14 b 69 c 160 f 350 f 620 i 540 b 410 a 230 a

50 43 a 24 b 100 d 170 f 310 f 630 f 670 j 400 a 200 a

75 61 a 33 b 130 e 180 f 290 f 530 f 730 i 390 a 190 a

100 78 a 41 b 150 e 190 f 280 g 460 f 770 c 390 a 180 a

150 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

200 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

250 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

300 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

350 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

400 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

Aluminum Criteria Look-Up Tables

27

bull EPA also provided criteria look-up tables in Appendix K

Har

dnes

s

CCC(microgL total aluminum)

(DOC=10 mgL)

pH 50 pH 55 pH 60 pH 65 pH 70 pH 75 pH 80 pH 85 pH 90

25 33 a 21 b 99 c 220 f 490 f 900 i 780 b 590 a 330 a

50 61 a 35 b 150 e 250 f 440 f 890 f 960 j 580 a 290 a

75 88 a 47 b 180 e 260 f 410 f 740 f 1100 i 570 a 270 a

100 11 a 59 b 210 e 270 f 390 g 650 f 1100 c 560 b 260 a

150 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

200 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

250 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

300 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

350 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

400 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

Aluminum Criteria Look-Up Tables

28

bull EPA also provided criteria look-up tables in Appendix K

Har

dnes

s

CCC(microgL total aluminum)

(DOC=25 mgL)

pH 50 pH 55 pH 60 pH 65 pH 70 pH 75 pH 80 pH 85 pH 90

25 54 a 34 b 160 c 350 f 770 f 1500 i 1300 b 960 a 540 a

50 99 a 57 b 230 e 390 f 690 f 1400 f 1600 j 930 a 470 a

75 14 a 77 b 290 e 410 f 640 f 1200 f 1700 i 920 a 440 a

100 18 a 96 b 330 e 420 f 620 g 1000 f 1800 d 920 b 420 a

150 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

200 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

250 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

300 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

350 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

400 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

Aluminum Criteria Look-Up Tables

29

bull EPA also provided criteria look-up tables in Appendix K

Har

dnes

s

CCC(microgL total aluminum)

(DOC=50 mgL)

pH 50 pH 55 pH 60 pH 65 pH 70 pH 75 pH 80 pH 85 pH 90

25 77 a 49 b 230 c 500 f 1100 f 2100 c 1900 b 1400 a 770 a

50 14 a 82 b 330 e 540 f 970 f 2000 f 2200 j 1300 a 680 a

75 20 a 110 b 410 e 570 f 900 f 1600 f 2500 i 1300 a 640 a

100 26 a 140 b 460 f 590 f 880 g 1400 f 2500 d 1300 b 600 a

150 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

200 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

250 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

300 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

350 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

400 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

Status

30

bull Draft Document Released to the Federal Register on 72817ndash Public comment period of 60 days ndash Due Date for Comments is 92617

bull Revisions will be made considering public commentsbull Subsequent EPA intra-agency review of final criteria

document will then be conducted bull Publication of final criteria document planned for Late Fall

2017

Federal Register Webpagehttpswwwfederalregistergovdocuments201

707282017-15968request-for-scientific-views-draft-updated-aquatic-life-ambient-water-quality-criteria-for-aluminum

Aluminum Criteria Webpagehttpswwwepagovwqc2017-draft-aquatic-life-

criteria-aluminum-freshwater-documents

31

	Appendix C Acceptable Chronic Toxicity Data of Aluminum to Freshwater Aquatic Animals
									MLR equation
	Species		Hardness (mgL as CaCO3)		pH		DOC(mgL)		EC20 Endpoint			EC20(microgL)			Normalized Conc- EC20(microgL)			SMCV-EC20(microgL)		GMCV-EC20(microgL)			Genus			DOC Notes		Dilution water
	Oligochaete Aeolosoma sp		48		6		025		Reproduction (population size)			1235			1710			1710		1710			Aeolosoma		Invert		Author reported half the detection limit
	Rotifer Brachionus calyciflorus		100		62		025		Reproduction (population size)			431			255			255		255			Brachionus		Invert		Author reported half the detection limit
	Great pond snail Lymnaea stagnalis		117		61		025		Biomass			746			488			488		488			Lymnaea		Mollusk		Author reported half the detection limit
	Fatmucket Lampsilis siliquoidea		1055		604		040		Biomass			169			104			104		104			Lampsilis		Mollusk		Author reported
	Cladoceran Ceriodaphnia dubia		50		715		11		Reproduction - youngstarting adult			1780			190						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		26		73		05		Reproduction - youngfemale			1557			272						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		46		75		05		Reproduction - youngfemale			8087			110						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		96		79		05		Reproduction - youngfemale			6519			80						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		194		81		05		Reproduction - youngfemale			6836			89						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		255		703		05		Reproduction (youngfemale)			250			56						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		122		713		05		Reproduction (youngfemale)			860			106						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		25		798		05		Reproduction (youngfemale)			700			101						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		61		803		05		Reproduction (youngfemale)			1010			136						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		122		810		05		Reproduction (youngfemale)			870			116						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		255		633		05		Reproduction (youngfemale)			260			176						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		121		636		05		Reproduction (youngfemale)			390			110			118		118			Ceriodaphnia		Invert		Author reported
	Amphipod Hyalella azteca		95		61		051		Biomass (28 d)			1993			101						Hyalella			Author reported
	Amphipod Hyalella azteca		106		604		033		Biomass (28 d)			425			289			171		171			Hyalella		Invert		Author reported
	Midge Chironomus riparius		118		558		18		Adult midge emergence			2955			119						Chironomus			Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Midge Chironomus riparius		119		505		18		Adult midge emergence			8442			2258						Chironomus			Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Midge Chironomus riparius		91		66		051		Reproduction ( of eggscase)			3387			766			591		591			Chironomus		Invert		Author reported
	Atlantic salmon Salmo salar		127		57		18		Biomass			6156			1112			1112		1112			Salmo		Fish		Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Brook trout Salvelinus fontinalis		123		655		19		Biomass			1644			59						Salvelinus			Author reported		wellRO mix
	Brook trout Salvelinus fontinalis		128		565		18		Biomass			1435			283			1293		1293			Salvelinus		Fish		Author reported		wellRO mix
	Fathead minnow Pimephales promelas		220		770		16		Biomass			6194			781						Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		well water
	Fathead minnow Pimephales promelas		96		620		025		Fry survival			4286			379			544		544			Pimephales		Fish		Author reported half the detection limit
	Zebrafish Danio rerio		83		61		025		Biomass			2344			241			241		241			Danio		Fish		Author reported half the detection limit
	Wood frog Rana sylvatica		115		469		16		MATC-survival		gt		2000		gt		2131		gt		2131		2131		gt		Rana		Amphib - Other Data		Value is from 2007 FW Cu AWQC Appendix C recommendation		aged tap water

	Appendix A Acceptable Acute Toxicity Data of Aluminum to Freshwater Aquatic Animals
	Species		Methoda		Hardness (mgL as CaCO3)		pH		DOC(mgL)			LC50 or EC50 Conc (microgL)		Reference			Normalized Conc - LC50(microgL)			Species Mean Acute Value(microgL)		Genus Mean Acute Value(microgL)			Genus			DOC Note		Dilution water
	Snail Physa sp		S M T		474		659		11		gt		23400		Call 1984 Call et al 1984		gt		4843			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		755		11			30600		Call 1984 Call et al 1984			2699			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		817		11		gt		24700		Call 1984 Call et al 1984		gt		2404			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		746		11			55500		Call 1984 Call et al 1984			5034			3546		3546			Physa		Mollusk		Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Fatmucket Lampsilis siliquoidea		F M T		1060		612		048		gt		6302		Wang et al 2016 Wang et al 2017		gt		2983		gt		2983		2983		gt		Lampsilis		Mollusk		Author reported
	Cladoceran Ceriodaphnia dubia		S M A		500		742		11			1900		McCauley et al 1986			173			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		S M A		505		786		11			1500		McCauley et al 1986			129			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		UW-S Lake Superior lab water
	Cladoceran Ceriodaphnia dubia		S M A		500		813		11			2560		McCauley et al 1986			243			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		R M T		26		75		05			720		ENSR 1992d			111			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		R M T		46		76		05			1880		ENSR 1992d			250			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		R M T		96		78		05			2450		ENSR 1992d			289			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		S U NR		90		715		05			3727		Fort and Stover 1995			500			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U NR		90		715		05			5673		Fort and Stover 1995			761			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U NR		89		76		05			2880		Soucek et al 2001			336			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		601		05			7112		European Al Association 2009			169			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		605		2			6865		European Al Association 2009			709			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		609		4			15581		European Al Association 2009			1007			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		601		05			681		European Al Association 2009			162			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		603		05			1630		European Al Association 2009			367			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		597		05			1785		European Al Association 2009			473			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		592		05			1410		European Al Association 2009			429			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		699		05		gt		1300		European Al Association 2009		gt		419			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		785		05		gt		5000		European Al Association 2009		gt		796			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		68		2		gt		10000		European Al Association 2009		gt		2076			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		782		2		gt		15000		European Al Association 2009		gt		1163			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		677		4		gt		10000		European Al Association 2009		gt		1516			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		766		4		gt		15000		European Al Association 2009		gt		861			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		79		05		gt		2000		European Al Association 2009		gt		315			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		789		05		gt		2000		European Al Association 2009		gt		316			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		604		05			1108		European Al Association 2009			85			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		598		2			11371		European Al Association 2009			482			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		573		4			80467		European Al Association 2009			4357			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		671		05		gt		10000		European Al Association 2009		gt		2379			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		783		05		gt		5000		European Al Association 2009		gt		634			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		679		2		gt		10000		European Al Association 2009		gt		1039			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		767		2		gt		15000		European Al Association 2009		gt		908			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		668		4		gt		15000		European Al Association 2009		gt		1246			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		762		4		gt		15000		European Al Association 2009		gt		633			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		606		2			33868		European Al Association 2009			792			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		56		4			104842		European Al Association 2009			4880			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		693		05		gt		5000		European Al Association 2009		gt		713			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		120		788		05		gt		5000		European Al Association 2009		gt		590			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		120		676		2		gt		15000		European Al Association 2009		gt		1212			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		771		2		gt		15000		European Al Association 2009		gt		810			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		66		4		gt		15000		European Al Association 2009		gt		1014			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		76		4		gt		15000		European Al Association 2009		gt		555			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			11971		European Al Association 2010			270			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			27478		European Al Association 2010			619			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			11998		European Al Association 2010			270			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		607		05			92495		European Al Association 2010			188			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		609		05			31337		European Al Association 2010			604			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		61		05			33235		European Al Association 2010			624			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		708		05		gt		8864		European Al Association 2010		gt		253			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		779		05		gt		42783		European Al Association 2010		gt		693			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		753		05			13204		European Al Association 2010			24			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		600		601		05			46326		European Al Association 2010			380			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		600		599		05		gt		8590		European Al Association 2010		gt		737			509				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia reticulata		F M T		451		60		11			304		Shephard 1983			201			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia reticulata		F M T		40		55		11			362		Shephard 1983			5531			1054		733			Ceriodaphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake SuperiorDI Mix
	Cladoceran Daphnia magna		S U NR		453		774		11			3900		Biesinger and Christensen 1972			338			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Daphnia magna		S M T		220		76		16			38200		Kimball 1978			2030			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Well water
	Cladoceran Daphnia magna		S U T		451		725		11			2800		Shephard 1983			287			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Daphnia magna		S U T		168		599		05			5000		European Al Association 2009		gt		227			-				Daphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Daphnia magna		S U T		168		792		05			7950		European Al Association 2009			92			333		333			Daphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Ostracod Stenocypris major		R M T		1563		651		16			310196		Shuhaimi-Othman et al 2011a			1022			1022		1022			Stenocypris		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (Malaysia)
	Amphipod Crangonyx pseudogracilis		R U T		50		675		16			9190		Martin and Holdich 1986			1217			1217		1217			Crangonyx		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (UK)
	Amphipod Hyalella azteca		F M T		105		613		048		gt		5997		Wang et al 2016 Wang et al 2017		gt		2800		gt		2800		2800		gt		Hyalella		Invert		Author reported
	Stonefly Acroneuria sp		S M T		474		746		11		gt		22600		Call et al 1984		gt		2050		gt		2050		2050		gt		Acroneuria		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Midge Chironomus plumosus		S U T		80		70		16			30000		Fargasova 2001			2536			2536		2536			Chironomus		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (Slovakia)
	Midge Paratanytarsus dissimilis		S M T		1743		728		28		gt		77700		Lamb and Bailey 1981 1983		gt		6232		gt		6232		6232		gt		Paratanytarsus		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Liberty Lake Washington
	Rainbow trout Oncorhynchus mykiss		F M T		274		758		05		gt		9840		Gundersen et al 1994		gt		1256			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		452		762		05		gt		8070		Gundersen et al 1994		gt		1140			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		895		758		05		gt		8160		Gundersen et al 1994		gt		1489			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		1304		758		05		gt		8200		Gundersen et al 1994		gt		1676			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		232		825		05			6170		Gundersen et al 1994			260			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		350		826		05			6170		Gundersen et al 1994			333			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		836		829		05			7670		Gundersen et al 1994			701			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		1158		830		05			6930		Gundersen et al 1994			777			801		801			Oncorhynchus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Atlantic salmon Salmo salar		S U T		68		55		05			584		Hamilton and Haines 1995			4602			-				Salmo			Value is from 2007 FW Cu AWQC Appendix C recommendation		Soft recon
	Atlantic salmon Salmo salar		S U T		68		65		05			599		Hamilton and Haines 1995			531			1564		1564			Salmo		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Soft recon
	Brook trout Salvelinus fontinalis		S U T		40		56		16			6530		Tandjung 1982			6947			-				Salvelinus			Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Brook trout Salvelinus fontinalis		S U T		18		56		16			3400		Tandjung 1982			6209			-				Salvelinus			Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Brook trout Salvelinus fontinalis		S U T		2		56		16			370		Tandjung 1982			2988			5051		5051			Salvelinus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Green sunfish Lepomis cyanellus		S M T		474		755		11		gt		50000		Call et al 1984		gt		5255		gt		5255		5255		gt		Lepomis		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Rio Grande silvery minnow Hybognathus amarus		R M T		140		81		05		gt		59100		Buhl 2002		gt		8620		gt		8620		8620		gt		Hybognathus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon water
	Fathead minnow Pimephales promelas		S M T		474		761		11		gt		48200		Call et al 1984		gt		4707			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Fathead minnow Pimephales promelas		S M T		474		805		11		gt		49800		Call et al 1984		gt		2837			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Fathead minnow Pimephales promelas		R M T		26		78		05			1160		ENSR 1992c			104			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		46		76		05			8180		ENSR 1992c			1191			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		96		81		05			20300		ENSR 1992c			2398			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		194		81		05			44800		ENSR 1992c			7839			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		140		81		05		gt		59100		Buhl 2002		gt		8620			2224		2224			Pimephales		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon water
	Smallmouth bass Micropterus dolomieui		S M T		1245		505		16			130		Kane and Rabeni 1987			859			859		859			Micropterus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Green tree frog Hyla cinerea		R M T		455		549		05		gt		4052		Jung and Jagoe 1995		gt		4377		gt		4377		4377		gt		Hyla		Amphibian		Value is from 2007 FW Cu AWQC Appendix C recommendation		artificial soft recon

	Normalization Chemistry
	pH		6					FAV		360
	Hardness		25					CMC		180
	DOC		1					CCC		99
	Acute Ranked GMAV											Chronic Ranked GMCV
	Rank			GMAV		Genus								Rank			GMCV		Genus
	18		gt		8620		Hybognathus		Fish							12		gt		2131		Rana		Amphib - Other Data
	17		gt		6232		Paratanytarsus		Invert							11			1710		Aeolosoma		Invert
	16		gt		5255		Lepomis		Fish							10			591		Chironomus		Invert
	15			5051		Salvelinus		Fish							9			544		Pimephales		Fish
	14		gt		4377		Hyla		Amphibian							8			488		Lymnaea		Mollusk
	13			3546		Physa		Mollusk							7			255		Brachionus		Invert
	12		gt		2983		Lampsilis		Mollusk							6			241		Danio		Fish
	11		gt		2800		Hyalella		Invert							5			171		Hyalella		Invert
	10			2536		Chironomus		Invert							4			129		Salvelinus		Fish
	9			2224		Pimephales		Fish							3			118		Ceriodaphnia		Invert
	8		gt		2050		Acroneuria		Invert							2			111		Salmo		Fish
	7			1564		Salmo		Fish							1			104		Lampsilis		Mollusk
	6			1217		Crangonyx		Invert
	5			1022		Stenocypris		Invert
	4			859		Micropterus		Fish
	3			801		Oncorhynchus		Fish
	2			733		Ceriodaphnia		Invert
	1			333		Daphnia		Invert
		N		Rank		Genus		GMAV		ln(GMAV)		ln(GMAV)2		P=R(N+1)		sqrt(P)				N		Rank		Genus		GMCV		ln(GMCV)		ln(GMCV)2		P=R(N+1)		sqrt(P)
		18		4		Micropterus		859		676		4564		0211		0459				12		4		Salvelinus		129		486		2364		0308		0555
			3		Oncorhynchus		801		669		4469		0158		0397					3		Ceriodaphnia		118		477		2277		0231		0480
			2		Ceriodaphnia		733		660		4352		0105		0324					2		Salmo		111		471		2220		0154		0392
			1		Daphnia		333		581		3374		0053		0229					1		Lampsilis		104		465		2159		0077		0277
			Sum				2585		1676		0526		141					Sum				1899		902		0769		170
			S2 =		1987										S2 =		059
			L =		4890										L =		4420
			A =		5887										A =		4592
			FAV =		360										FCV =		987
			CMC =		180

				All concentrations reported are microgL total Aluminum
				lt-----Click Button after entering data
	ENTER DATA HERE									ERRORNUM		ACUTE														CHRONIC
										Rank 1				Rank 2				Rank 3				Rank 4					Rank 1				Rank 2				Rank 3				Rank 4
	SiteName		DOC(mgL)		Hardness(mgL as CaCO3)		pH			FAV		CMC		CCC			Genus		GMAV			Genus		GMAV			Genus		GMAV			Genus		GMAV				Genus		GMCV			Genus		GMCV			Genus		GMCV			Genus		GMCV
	Site 1		50		25		9		75428737034162		35229324666008		1800		770			Daphnia			33452		Ceriodaphnia			73576		Stenocypris			102576		Crangonyx			122230			Lampsilis			10463		Ceriodaphnia			11865		Hyalella			17200		Brachionus			25648
	Site 2		50		50		9		75428737034162		31204991509063		1600		680			Daphnia			29631		Ceriodaphnia			65171		Stenocypris			90859		Crangonyx			108267			Lampsilis			9268		Ceriodaphnia			10510		Hyalella			15236		Brachionus			22718
	Site 3		50		75		9		75428737034162		29067527928199		1500		640			Daphnia			27601		Ceriodaphnia			60707		Stenocypris			84635		Crangonyx			100851			Lampsilis			8633		Ceriodaphnia			9790		Hyalella			14192		Brachionus			21162
	Site 4		50		100		9		75428737034162		27640367912592		1400		600			Daphnia			26246		Ceriodaphnia			57726		Stenocypris			80480		Crangonyx			95899			Lampsilis			8209		Ceriodaphnia			9309		Hyalella			13495		Brachionus			20123
	Site 5		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 6		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 7		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 8		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 9		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 10		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 11		50		25		85		75428737034162		63245275664384		3200		1400			Daphnia			60055		Ceriodaphnia			132086		Stenocypris			184149		Crangonyx			219432			Lampsilis			18783		Ceriodaphnia			21301		Hyalella			30879		Brachionus			46045
	Site 12		50		50		85		75428737034162		61388044609926		3100		1300			Daphnia			58291		Ceriodaphnia			128208		Stenocypris			178742		Crangonyx			212988			Lampsilis			18232		Ceriodaphnia			20676		Hyalella			29972		Brachionus			44693
	Site 13		50		75		85		75428737034162		60327020429132		3000		1300			Daphnia			57284		Ceriodaphnia			125992		Stenocypris			175652		Crangonyx			209307			Lampsilis			17917		Ceriodaphnia			20318		Hyalella			29454		Brachionus			43920
	Site 14		50		100		85		75428737034162		59585352130146		3000		1300			Daphnia			56580		Ceriodaphnia			124443		Stenocypris			173493		Crangonyx			206734			Lampsilis			17696		Ceriodaphnia			20068		Hyalella			29092		Salmo			41264
	Site 15		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 16		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 17		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 18		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 19		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 20		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370

	Appendix C Acceptable Chronic Toxicity Data of Aluminum to Freshwater Aquatic Animals
									MLR equation
	Species		Hardness (mgL as CaCO3)		pH		DOC(mgL)		EC20 Endpoint			EC20(microgL)			Normalized Conc- EC20(microgL)			SMCV-EC20(microgL)		GMCV-EC20(microgL)			Genus			DOC Notes		Dilution water
	Oligochaete Aeolosoma sp		48		6		025		Reproduction (population size)			1235			17098			17098		17098			Aeolosoma		Invert		Author reported half the detection limit
	Rotifer Brachionus calyciflorus		100		62		025		Reproduction (population size)			431			2555			2555		2555			Brachionus		Invert		Author reported half the detection limit
	Great pond snail Lymnaea stagnalis		117		61		025		Biomass			746			4877			4877		4877			Lymnaea		Mollusk		Author reported half the detection limit
	Fatmucket Lampsilis siliquoidea		1055		604		040		Biomass			169			1042			1042		1042			Lampsilis		Mollusk		Author reported
	Cladoceran Ceriodaphnia dubia		50		715		11		Reproduction - youngstarting adult			1780			1898						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		26		73		05		Reproduction - youngfemale			1557			2718						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		46		75		05		Reproduction - youngfemale			8087			1102						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		96		79		05		Reproduction - youngfemale			6519			796						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		194		81		05		Reproduction - youngfemale			6836			888						Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon
	Cladoceran Ceriodaphnia dubia		255		703		05		Reproduction (youngfemale)			250			563						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		122		713		05		Reproduction (youngfemale)			860			1061						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		25		798		05		Reproduction (youngfemale)			700			1006						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		61		803		05		Reproduction (youngfemale)			1010			1363						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		122		810		05		Reproduction (youngfemale)			870			1164						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		255		633		05		Reproduction (youngfemale)			260			1758						Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		121		636		05		Reproduction (youngfemale)			390			1101			1182		1182			Ceriodaphnia		Invert		Author reported
	Amphipod Hyalella azteca		95		61		051		Biomass (28 d)			1993			1014						Hyalella			Author reported
	Amphipod Hyalella azteca		106		604		033		Biomass (28 d)			425			2895			1713		1713			Hyalella		Invert		Author reported
	Midge Chironomus riparius		118		558		18		Adult midge emergence			2955			1192						Chironomus			Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Midge Chironomus riparius		119		505		18		Adult midge emergence			8442			22578						Chironomus			Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Midge Chironomus riparius		91		66		051		Reproduction ( of eggscase)			3387			7664			5908		5908			Chironomus		Invert		Author reported
	Atlantic salmon Salmo salar		127		57		18		Biomass			6156			5085			5085		5085			Salmo		Fish		Based on Cleveland et al 1989 reported values at a similar pH		wellRO mix
	Brook trout Salvelinus fontinalis		123		655		19		Biomass			1644			270						Salvelinus			Author reported		wellRO mix
	Brook trout Salvelinus fontinalis		128		565		18		Biomass			1435			1295			5914		5914			Salvelinus		Fish		Author reported		wellRO mix
	Fathead minnow Pimephales promelas		220		770		16		Biomass			6194			3569						Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		well water
	Fathead minnow Pimephales promelas		96		620		025		Fry survival			4286			1734			2488		2488			Pimephales		Fish		Author reported half the detection limit
	Zebrafish Danio rerio		83		61		025		Biomass			2344			1102			1102		1102			Danio		Fish		Author reported half the detection limit
	Wood frog Rana sylvatica		115		469		16		MATC-survival		gt		2000		gt		9746		gt		9746		9746		gt		Rana		Amphib - Other Data		Value is from 2007 FW Cu AWQC Appendix C recommendation		aged tap water

	Appendix A Acceptable Acute Toxicity Data of Aluminum to Freshwater Aquatic Animals
	Species		Methoda		Hardness (mgL as CaCO3)		pH		DOC(mgL)			LC50 or EC50 Conc (microgL)		Reference			Normalized Conc - LC50(microgL)			Species Mean Acute Value(microgL)		Genus Mean Acute Value(microgL)			Genus			DOC Note		Dilution water
	Snail Physa sp		S M T		474		659		11		gt		23400		Call 1984 Call et al 1984		gt		48425			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		755		11			30600		Call 1984 Call et al 1984			26986			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		817		11		gt		24700		Call 1984 Call et al 1984		gt		24040			-				Physa			Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Snail Physa sp		S M T		474		746		11			55500		Call 1984 Call et al 1984			50338			35462		35462			Physa		Mollusk		Value is from 2007 FW Cu AWQC Appendix C recommendation		Raw Lake Superior water
	Fatmucket Lampsilis siliquoidea		F M T		1060		612		048		gt		6302		Wang et al 2016 Wang et al 2017		gt		29834		gt		29834		29834		gt		Lampsilis		Mollusk		Author reported
	Cladoceran Ceriodaphnia dubia		S M A		500		742		11			1900		McCauley et al 1986			1728			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		S M A		505		786		11			1500		McCauley et al 1986			1294			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		UW-S Lake Superior lab water
	Cladoceran Ceriodaphnia dubia		S M A		500		813		11			2560		McCauley et al 1986			2431			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia dubia		R M T		26		75		05			720		ENSR 1992d			1113			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		R M T		46		76		05			1880		ENSR 1992d			2495			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		R M T		96		78		05			2450		ENSR 1992d			2894			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Cladoceran Ceriodaphnia dubia		S U NR		90		715		05			3727		Fort and Stover 1995			5002			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U NR		90		715		05			5673		Fort and Stover 1995			7613			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U NR		89		76		05			2880		Soucek et al 2001			3362			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Mod Hard Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		601		05			7112		European Al Association 2009			1691			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		605		2			6865		European Al Association 2009			7088			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		609		4			15581		European Al Association 2009			10072			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		601		05			681		European Al Association 2009			1620			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		603		05			1630		European Al Association 2009			3674			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		597		05			1785		European Al Association 2009			4732			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		592		05			1410		European Al Association 2009			4295			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		699		05		gt		1300		European Al Association 2009		gt		4189			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		785		05		gt		5000		European Al Association 2009		gt		7960			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		68		2		gt		10000		European Al Association 2009		gt		20755			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		782		2		gt		15000		European Al Association 2009		gt		11626			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		677		4		gt		10000		European Al Association 2009		gt		15161			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		766		4		gt		15000		European Al Association 2009		gt		8608			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		106		79		05		gt		2000		European Al Association 2009		gt		3150			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		106		789		05		gt		2000		European Al Association 2009		gt		3156			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		604		05			1108		European Al Association 2009			850			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		598		2			11371		European Al Association 2009			4817			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		573		4			80467		European Al Association 2009			43570			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		671		05		gt		10000		European Al Association 2009		gt		23786			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		783		05		gt		5000		European Al Association 2009		gt		6344			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		60		679		2		gt		10000		European Al Association 2009		gt		10394			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		767		2		gt		15000		European Al Association 2009		gt		9082			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		668		4		gt		15000		European Al Association 2009		gt		12461			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		60		762		4		gt		15000		European Al Association 2009		gt		6332			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		606		2			33868		European Al Association 2009			7920			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		56		4			104842		European Al Association 2009			48800			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		693		05		gt		5000		European Al Association 2009		gt		7126			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		120		788		05		gt		5000		European Al Association 2009		gt		5898			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S U T		120		676		2		gt		15000		European Al Association 2009		gt		12117			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		771		2		gt		15000		European Al Association 2009		gt		8096			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		66		4		gt		15000		European Al Association 2009		gt		10139			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S U T		120		76		4		gt		15000		European Al Association 2009		gt		5545			-				Ceriodaphnia			Author reported
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			11971		European Al Association 2010			2699			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			27478		European Al Association 2010			6194			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		603		05			11998		European Al Association 2010			2705			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		607		05			92495		European Al Association 2010			1876			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		609		05			31337		European Al Association 2010			6035			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		61		05			33235		European Al Association 2010			6238			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		708		05		gt		8864		European Al Association 2010		gt		2534			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		779		05		gt		42783		European Al Association 2010		gt		6930			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		106		753		05			13204		European Al Association 2010			244			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		600		601		05			46326		European Al Association 2010			3798			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia dubia		S M T		600		599		05		gt		8590		European Al Association 2010		gt		7366			5094				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Ceriodaphnia reticulata		F M T		451		60		11			304		Shephard 1983			2009			-				Ceriodaphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Ceriodaphnia reticulata		F M T		40		55		11			362		Shephard 1983			55307			10542		7328			Ceriodaphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake SuperiorDI Mix
	Cladoceran Daphnia magna		S U NR		453		774		11			3900		Biesinger and Christensen 1972			3382			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Daphnia magna		S M T		220		76		16			38200		Kimball 1978			20303			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Well water
	Cladoceran Daphnia magna		S U T		451		725		11			2800		Shephard 1983			2868			-				Daphnia			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Cladoceran Daphnia magna		S U T		168		599		05			5000		European Al Association 2009		gt		2265			-				Daphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Cladoceran Daphnia magna		S U T		168		792		05			7950		European Al Association 2009			920			3332		3332			Daphnia		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon
	Ostracod Stenocypris major		R M T		1563		651		16			310196		Shuhaimi-Othman et al 2011a			10216			10216		10216			Stenocypris		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (Malaysia)
	Amphipod Crangonyx pseudogracilis		R U T		50		675		16			9190		Martin and Holdich 1986			12174			12174		12174			Crangonyx		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (UK)
	Amphipod Hyalella azteca		F M T		105		613		048		gt		5997		Wang et al 2016 Wang et al 2017		gt		28002		gt		28002		28002		gt		Hyalella		Invert		Author reported
	Stonefly Acroneuria sp		S M T		474		746		11		gt		22600		Call et al 1984		gt		20498		gt		20498		20498		gt		Acroneuria		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Midge Chironomus plumosus		S U T		80		70		16			30000		Fargasova 2001			25361			25361		25361			Chironomus		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		tap water (Slovakia)
	Midge Paratanytarsus dissimilis		S M T		1743		728		28		gt		77700		Lamb and Bailey 1981 1983		gt		62318		gt		62318		62318		gt		Paratanytarsus		Invert		Value is from 2007 FW Cu AWQC Appendix C recommendation		Liberty Lake Washington
	Rainbow trout Oncorhynchus mykiss		F M T		274		758		05		gt		9840		Gundersen et al 1994		gt		5745			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		452		762		05		gt		8070		Gundersen et al 1994		gt		5212			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		895		758		05		gt		8160		Gundersen et al 1994		gt		6807			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		1304		758		05		gt		8200		Gundersen et al 1994		gt		7662			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		232		825		05			6170		Gundersen et al 1994			1190			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		350		826		05			6170		Gundersen et al 1994			1523			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		836		829		05			7670		Gundersen et al 1994			3205			-				Oncorhynchus			Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Rainbow trout Oncorhynchus mykiss		F M T		1158		830		05			6930		Gundersen et al 1994			3554			3661		3661			Oncorhynchus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		RO treated well water then added salts
	Atlantic salmon Salmo salar		S U T		68		55		05			584		Hamilton and Haines 1995			21042			-				Salmo			Value is from 2007 FW Cu AWQC Appendix C recommendation		Soft recon
	Atlantic salmon Salmo salar		S U T		68		65		05			599		Hamilton and Haines 1995			2430			7151		7151			Salmo		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Soft recon
	Brook trout Salvelinus fontinalis		S U T		40		56		16			6530		Tandjung 1982			31767			-				Salvelinus			Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Brook trout Salvelinus fontinalis		S U T		18		56		16			3400		Tandjung 1982			28391			-				Salvelinus			Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Brook trout Salvelinus fontinalis		S U T		2		56		16			370		Tandjung 1982			13663			23097		23097			Salvelinus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Green sunfish Lepomis cyanellus		S M T		474		755		11		gt		50000		Call et al 1984		gt		24028		gt		24028		24028		gt		Lepomis		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Rio Grande silvery minnow Hybognathus amarus		R M T		140		81		05		gt		59100		Buhl 2002		gt		39414		gt		39414		39414		gt		Hybognathus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon water
	Fathead minnow Pimephales promelas		S M T		474		761		11		gt		48200		Call et al 1984		gt		21522			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Fathead minnow Pimephales promelas		S M T		474		805		11		gt		49800		Call et al 1984		gt		12972			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lake Superior water
	Fathead minnow Pimephales promelas		R M T		26		78		05			1160		ENSR 1992c			477			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		46		76		05			8180		ENSR 1992c			5446			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		96		81		05			20300		ENSR 1992c			10966			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		194		81		05			44800		ENSR 1992c			35847			-				Pimephales			Value is from 2007 FW Cu AWQC Appendix C recommendation		Lab Recon water
	Fathead minnow Pimephales promelas		R M T		140		81		05		gt		59100		Buhl 2002		gt		39414			10168		10168			Pimephales		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		Recon water
	Smallmouth bass Micropterus dolomieui		S M T		1245		505		16			130		Kane and Rabeni 1987			3929			3929		3929			Micropterus		Fish		Value is from 2007 FW Cu AWQC Appendix C recommendation		well waterDI mix
	Green tree frog Hyla cinerea		R M T		455		549		05		gt		4052		Jung and Jagoe 1995		gt		20016		gt		20016		20016		gt		Hyla		Amphibian		Value is from 2007 FW Cu AWQC Appendix C recommendation		artificial soft recon

	Normalization Chemistry
	pH		7					FAV		2741
	Hardness		100					CMC		1400
	DOC		1					CCC		390
	Acute Ranked GMAV											Chronic Ranked GMCV
	Rank			GMAV		Genus								Rank			GMCV		Genus
	18		gt		62318		Paratanytarsus		Invert							12			17098		Aeolosoma		Invert
	17		gt		39414		Hybognathus		Fish							11		gt		9746		Rana		Amphib - Other Data
	16			35462		Physa		Mollusk							10			5908		Chironomus		Invert
	15		gt		29834		Lampsilis		Mollusk							9			4877		Lymnaea		Mollusk
	14		gt		28002		Hyalella		Invert							8			2555		Brachionus		Invert
	13			25361		Chironomus		Invert							7			2488		Pimephales		Fish
	12		gt		24028		Lepomis		Fish							6			1713		Hyalella		Invert
	11			23097		Salvelinus		Fish							5			1182		Ceriodaphnia		Invert
	10		gt		20498		Acroneuria		Invert							4			1102		Danio		Fish
	9		gt		20016		Hyla		Amphibian							3			1042		Lampsilis		Mollusk
	8			12174		Crangonyx		Invert							2			591		Salvelinus		Fish
	7			10216		Stenocypris		Invert							1			509		Salmo		Fish
	6			10168		Pimephales		Fish
	5			7328		Ceriodaphnia		Invert
	4			7151		Salmo		Fish
	3			3929		Micropterus		Fish
	2			3661		Oncorhynchus		Fish
	1			3332		Daphnia		Invert
		N		Rank		Genus		GMAV		ln(GMAV)		ln(GMAV)2		P=R(N+1)		sqrt(P)				N		Rank		Genus		GMCV		ln(GMCV)		ln(GMCV)2		P=R(N+1)		sqrt(P)
		18		4		Salmo		7151		887		7876		0211		0459				12		4		Danio		1102		700		4907		0308		0555
			3		Micropterus		3929		828		6849		0158		0397					3		Lampsilis		1042		695		4829		0231		0480
			2		Oncorhynchus		3661		821		6733		0105		0324					2		Salvelinus		591		638		4074		0154		0392
			1		Daphnia		3332		811		6579		0053		0229					1		Salmo		509		623		3883		0077		0277
			Sum				3347		2804		0526		141					Sum				2657		1769		0769		170
			S2 =		1223										S2 =		1080
			L =		7134										L =		5242
			A =		7916										A =		5976
			FAV =		2741										FCV =		3940
			CMC =		1371

				All concentrations reported are microgL total Aluminum
				lt-----Click Button after entering data
	ENTER DATA HERE									ERRORNUM		ACUTE														CHRONIC
										Rank 1				Rank 2				Rank 3				Rank 4					Rank 1				Rank 2				Rank 3				Rank 4
	SiteName		DOC(mgL)		Hardness(mgL as CaCO3)		pH			FAV		CMC		CCC			Genus		GMAV			Genus		GMAV			Genus		GMAV			Genus		GMAV				Genus		GMCV			Genus		GMCV			Genus		GMCV			Genus		GMCV
	Site 1		50		25		9		75428737034162		35229324666008		1800		770			Daphnia			33452		Ceriodaphnia			73576		Stenocypris			102576		Crangonyx			122230			Lampsilis			10463		Ceriodaphnia			11865		Hyalella			17200		Brachionus			25648
	Site 2		50		50		9		75428737034162		31204991509063		1600		680			Daphnia			29631		Ceriodaphnia			65171		Stenocypris			90859		Crangonyx			108267			Lampsilis			9268		Ceriodaphnia			10510		Hyalella			15236		Brachionus			22718
	Site 3		50		75		9		75428737034162		29067527928199		1500		640			Daphnia			27601		Ceriodaphnia			60707		Stenocypris			84635		Crangonyx			100851			Lampsilis			8633		Ceriodaphnia			9790		Hyalella			14192		Brachionus			21162
	Site 4		50		100		9		75428737034162		27640367912592		1400		600			Daphnia			26246		Ceriodaphnia			57726		Stenocypris			80480		Crangonyx			95899			Lampsilis			8209		Ceriodaphnia			9309		Hyalella			13495		Brachionus			20123
	Site 5		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 6		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 7		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 8		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 9		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 10		50		150		9		75428737034162		25747072099401		1300		560			Daphnia			24448		Ceriodaphnia			53772		Stenocypris			74967		Crangonyx			89330			Lampsilis			7647		Ceriodaphnia			8672		Hyalella			12571		Brachionus			18745
	Site 11		50		25		85		75428737034162		63245275664384		3200		1400			Daphnia			60055		Ceriodaphnia			132086		Stenocypris			184149		Crangonyx			219432			Lampsilis			18783		Ceriodaphnia			21301		Hyalella			30879		Brachionus			46045
	Site 12		50		50		85		75428737034162		61388044609926		3100		1300			Daphnia			58291		Ceriodaphnia			128208		Stenocypris			178742		Crangonyx			212988			Lampsilis			18232		Ceriodaphnia			20676		Hyalella			29972		Brachionus			44693
	Site 13		50		75		85		75428737034162		60327020429132		3000		1300			Daphnia			57284		Ceriodaphnia			125992		Stenocypris			175652		Crangonyx			209307			Lampsilis			17917		Ceriodaphnia			20318		Hyalella			29454		Brachionus			43920
	Site 14		50		100		85		75428737034162		59585352130146		3000		1300			Daphnia			56580		Ceriodaphnia			124443		Stenocypris			173493		Crangonyx			206734			Lampsilis			17696		Ceriodaphnia			20068		Hyalella			29092		Salmo			41264
	Site 15		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 16		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 17		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 18		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 19		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370
	Site 20		50		150		85		75428737034162		58555485487011		2900		1500			Daphnia			55602		Ceriodaphnia			122292		Stenocypris			170494		Crangonyx			203161			Lampsilis			17391		Ceriodaphnia			19722		Hyalella			28589		Salmo			30370

Boundaries of the Criteria Outputsbull MLR models were developed using data that

encompassndash DOC range of 008-5 mgLndash Hardness range of 98-127 mgL (as CaCO3)ndash pH range of 6-81

bull MLR criteria outputs are bounded atndash Maximum of 150 mgL total hardness (as CaCO3)

ndash DOC of 50 mgLndash pH range of the model is 50 to 90

25

Aluminum Criteria Look-Up Tables

26

bull EPA also provided criteria look-up tables in Appendix K

Har

dnes

s

CCC(microgL total aluminum)

(DOC=05 mgL)

pH 50 pH 55 pH 60 pH 65 pH 70 pH 75 pH 80 pH 85 pH 90

25 23 a 14 b 69 c 160 f 350 f 620 i 540 b 410 a 230 a

50 43 a 24 b 100 d 170 f 310 f 630 f 670 j 400 a 200 a

75 61 a 33 b 130 e 180 f 290 f 530 f 730 i 390 a 190 a

100 78 a 41 b 150 e 190 f 280 g 460 f 770 c 390 a 180 a

150 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

200 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

250 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

300 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

350 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

400 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

Aluminum Criteria Look-Up Tables

27

bull EPA also provided criteria look-up tables in Appendix K

Har

dnes

s

CCC(microgL total aluminum)

(DOC=10 mgL)

pH 50 pH 55 pH 60 pH 65 pH 70 pH 75 pH 80 pH 85 pH 90

25 33 a 21 b 99 c 220 f 490 f 900 i 780 b 590 a 330 a

50 61 a 35 b 150 e 250 f 440 f 890 f 960 j 580 a 290 a

75 88 a 47 b 180 e 260 f 410 f 740 f 1100 i 570 a 270 a

100 11 a 59 b 210 e 270 f 390 g 650 f 1100 c 560 b 260 a

150 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

200 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

250 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

300 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

350 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

400 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

Aluminum Criteria Look-Up Tables

28

bull EPA also provided criteria look-up tables in Appendix K

Har

dnes

s

CCC(microgL total aluminum)

(DOC=25 mgL)

pH 50 pH 55 pH 60 pH 65 pH 70 pH 75 pH 80 pH 85 pH 90

25 54 a 34 b 160 c 350 f 770 f 1500 i 1300 b 960 a 540 a

50 99 a 57 b 230 e 390 f 690 f 1400 f 1600 j 930 a 470 a

75 14 a 77 b 290 e 410 f 640 f 1200 f 1700 i 920 a 440 a

100 18 a 96 b 330 e 420 f 620 g 1000 f 1800 d 920 b 420 a

150 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

200 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

250 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

300 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

350 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

400 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

Aluminum Criteria Look-Up Tables

29

bull EPA also provided criteria look-up tables in Appendix K

Har

dnes

s

CCC(microgL total aluminum)

(DOC=50 mgL)

pH 50 pH 55 pH 60 pH 65 pH 70 pH 75 pH 80 pH 85 pH 90

25 77 a 49 b 230 c 500 f 1100 f 2100 c 1900 b 1400 a 770 a

50 14 a 82 b 330 e 540 f 970 f 2000 f 2200 j 1300 a 680 a

75 20 a 110 b 410 e 570 f 900 f 1600 f 2500 i 1300 a 640 a

100 26 a 140 b 460 f 590 f 880 g 1400 f 2500 d 1300 b 600 a

150 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

200 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

250 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

300 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

350 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

400 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

Status

30

bull Draft Document Released to the Federal Register on 72817ndash Public comment period of 60 days ndash Due Date for Comments is 92617

bull Revisions will be made considering public commentsbull Subsequent EPA intra-agency review of final criteria

document will then be conducted bull Publication of final criteria document planned for Late Fall

2017

Federal Register Webpagehttpswwwfederalregistergovdocuments201

707282017-15968request-for-scientific-views-draft-updated-aquatic-life-ambient-water-quality-criteria-for-aluminum

Aluminum Criteria Webpagehttpswwwepagovwqc2017-draft-aquatic-life-

criteria-aluminum-freshwater-documents

31

Aluminum Criteria Look-Up Tables

26

bull EPA also provided criteria look-up tables in Appendix K

Har

dnes

s

CCC(microgL total aluminum)

(DOC=05 mgL)

pH 50 pH 55 pH 60 pH 65 pH 70 pH 75 pH 80 pH 85 pH 90

25 23 a 14 b 69 c 160 f 350 f 620 i 540 b 410 a 230 a

50 43 a 24 b 100 d 170 f 310 f 630 f 670 j 400 a 200 a

75 61 a 33 b 130 e 180 f 290 f 530 f 730 i 390 a 190 a

100 78 a 41 b 150 e 190 f 280 g 460 f 770 c 390 a 180 a

150 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

200 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

250 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

300 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

350 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

400 11 a 55 b 180 f 200 g 270 h 390 g 650 f 430 b 170 a

Aluminum Criteria Look-Up Tables

27

bull EPA also provided criteria look-up tables in Appendix K

Har

dnes

s

CCC(microgL total aluminum)

(DOC=10 mgL)

pH 50 pH 55 pH 60 pH 65 pH 70 pH 75 pH 80 pH 85 pH 90

25 33 a 21 b 99 c 220 f 490 f 900 i 780 b 590 a 330 a

50 61 a 35 b 150 e 250 f 440 f 890 f 960 j 580 a 290 a

75 88 a 47 b 180 e 260 f 410 f 740 f 1100 i 570 a 270 a

100 11 a 59 b 210 e 270 f 390 g 650 f 1100 c 560 b 260 a

150 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

200 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

250 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

300 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

350 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

400 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

Aluminum Criteria Look-Up Tables

28

bull EPA also provided criteria look-up tables in Appendix K

Har

dnes

s

CCC(microgL total aluminum)

(DOC=25 mgL)

pH 50 pH 55 pH 60 pH 65 pH 70 pH 75 pH 80 pH 85 pH 90

25 54 a 34 b 160 c 350 f 770 f 1500 i 1300 b 960 a 540 a

50 99 a 57 b 230 e 390 f 690 f 1400 f 1600 j 930 a 470 a

75 14 a 77 b 290 e 410 f 640 f 1200 f 1700 i 920 a 440 a

100 18 a 96 b 330 e 420 f 620 g 1000 f 1800 d 920 b 420 a

150 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

200 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

250 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

300 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

350 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

400 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

Aluminum Criteria Look-Up Tables

29

bull EPA also provided criteria look-up tables in Appendix K

Har

dnes

s

CCC(microgL total aluminum)

(DOC=50 mgL)

pH 50 pH 55 pH 60 pH 65 pH 70 pH 75 pH 80 pH 85 pH 90

25 77 a 49 b 230 c 500 f 1100 f 2100 c 1900 b 1400 a 770 a

50 14 a 82 b 330 e 540 f 970 f 2000 f 2200 j 1300 a 680 a

75 20 a 110 b 410 e 570 f 900 f 1600 f 2500 i 1300 a 640 a

100 26 a 140 b 460 f 590 f 880 g 1400 f 2500 d 1300 b 600 a

150 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

200 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

250 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

300 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

350 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

400 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

Status

30

bull Draft Document Released to the Federal Register on 72817ndash Public comment period of 60 days ndash Due Date for Comments is 92617

bull Revisions will be made considering public commentsbull Subsequent EPA intra-agency review of final criteria

document will then be conducted bull Publication of final criteria document planned for Late Fall

2017

Federal Register Webpagehttpswwwfederalregistergovdocuments201

707282017-15968request-for-scientific-views-draft-updated-aquatic-life-ambient-water-quality-criteria-for-aluminum

Aluminum Criteria Webpagehttpswwwepagovwqc2017-draft-aquatic-life-

criteria-aluminum-freshwater-documents

31

Aluminum Criteria Look-Up Tables

27

bull EPA also provided criteria look-up tables in Appendix K

Har

dnes

s

CCC(microgL total aluminum)

(DOC=10 mgL)

pH 50 pH 55 pH 60 pH 65 pH 70 pH 75 pH 80 pH 85 pH 90

25 33 a 21 b 99 c 220 f 490 f 900 i 780 b 590 a 330 a

50 61 a 35 b 150 e 250 f 440 f 890 f 960 j 580 a 290 a

75 88 a 47 b 180 e 260 f 410 f 740 f 1100 i 570 a 270 a

100 11 a 59 b 210 e 270 f 390 g 650 f 1100 c 560 b 260 a

150 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

200 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

250 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

300 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

350 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

400 16 a 80 b 250 f 280 g 380 h 540 g 910 f 620 b 240 a

Aluminum Criteria Look-Up Tables

28

bull EPA also provided criteria look-up tables in Appendix K

Har

dnes

s

CCC(microgL total aluminum)

(DOC=25 mgL)

pH 50 pH 55 pH 60 pH 65 pH 70 pH 75 pH 80 pH 85 pH 90

25 54 a 34 b 160 c 350 f 770 f 1500 i 1300 b 960 a 540 a

50 99 a 57 b 230 e 390 f 690 f 1400 f 1600 j 930 a 470 a

75 14 a 77 b 290 e 410 f 640 f 1200 f 1700 i 920 a 440 a

100 18 a 96 b 330 e 420 f 620 g 1000 f 1800 d 920 b 420 a

150 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

200 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

250 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

300 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

350 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

400 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

Aluminum Criteria Look-Up Tables

29

bull EPA also provided criteria look-up tables in Appendix K

Har

dnes

s

CCC(microgL total aluminum)

(DOC=50 mgL)

pH 50 pH 55 pH 60 pH 65 pH 70 pH 75 pH 80 pH 85 pH 90

25 77 a 49 b 230 c 500 f 1100 f 2100 c 1900 b 1400 a 770 a

50 14 a 82 b 330 e 540 f 970 f 2000 f 2200 j 1300 a 680 a

75 20 a 110 b 410 e 570 f 900 f 1600 f 2500 i 1300 a 640 a

100 26 a 140 b 460 f 590 f 880 g 1400 f 2500 d 1300 b 600 a

150 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

200 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

250 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

300 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

350 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

400 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

Status

30

bull Draft Document Released to the Federal Register on 72817ndash Public comment period of 60 days ndash Due Date for Comments is 92617

bull Revisions will be made considering public commentsbull Subsequent EPA intra-agency review of final criteria

document will then be conducted bull Publication of final criteria document planned for Late Fall

2017

Federal Register Webpagehttpswwwfederalregistergovdocuments201

707282017-15968request-for-scientific-views-draft-updated-aquatic-life-ambient-water-quality-criteria-for-aluminum

Aluminum Criteria Webpagehttpswwwepagovwqc2017-draft-aquatic-life-

criteria-aluminum-freshwater-documents

31

Aluminum Criteria Look-Up Tables

28

bull EPA also provided criteria look-up tables in Appendix K

Har

dnes

s

CCC(microgL total aluminum)

(DOC=25 mgL)

pH 50 pH 55 pH 60 pH 65 pH 70 pH 75 pH 80 pH 85 pH 90

25 54 a 34 b 160 c 350 f 770 f 1500 i 1300 b 960 a 540 a

50 99 a 57 b 230 e 390 f 690 f 1400 f 1600 j 930 a 470 a

75 14 a 77 b 290 e 410 f 640 f 1200 f 1700 i 920 a 440 a

100 18 a 96 b 330 e 420 f 620 g 1000 f 1800 d 920 b 420 a

150 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

200 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

250 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

300 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

350 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

400 26 a 130 b 390 f 450 g 590 h 860 g 1400 f 1000 b 390 a

Aluminum Criteria Look-Up Tables

29

bull EPA also provided criteria look-up tables in Appendix K

Har

dnes

s

CCC(microgL total aluminum)

(DOC=50 mgL)

pH 50 pH 55 pH 60 pH 65 pH 70 pH 75 pH 80 pH 85 pH 90

25 77 a 49 b 230 c 500 f 1100 f 2100 c 1900 b 1400 a 770 a

50 14 a 82 b 330 e 540 f 970 f 2000 f 2200 j 1300 a 680 a

75 20 a 110 b 410 e 570 f 900 f 1600 f 2500 i 1300 a 640 a

100 26 a 140 b 460 f 590 f 880 g 1400 f 2500 d 1300 b 600 a

150 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

200 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

250 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

300 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

350 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

400 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

Status

30

bull Draft Document Released to the Federal Register on 72817ndash Public comment period of 60 days ndash Due Date for Comments is 92617

bull Revisions will be made considering public commentsbull Subsequent EPA intra-agency review of final criteria

document will then be conducted bull Publication of final criteria document planned for Late Fall

2017

Federal Register Webpagehttpswwwfederalregistergovdocuments201

707282017-15968request-for-scientific-views-draft-updated-aquatic-life-ambient-water-quality-criteria-for-aluminum

Aluminum Criteria Webpagehttpswwwepagovwqc2017-draft-aquatic-life-

criteria-aluminum-freshwater-documents

31

Aluminum Criteria Look-Up Tables

29

bull EPA also provided criteria look-up tables in Appendix K

Har

dnes

s

CCC(microgL total aluminum)

(DOC=50 mgL)

pH 50 pH 55 pH 60 pH 65 pH 70 pH 75 pH 80 pH 85 pH 90

25 77 a 49 b 230 c 500 f 1100 f 2100 c 1900 b 1400 a 770 a

50 14 a 82 b 330 e 540 f 970 f 2000 f 2200 j 1300 a 680 a

75 20 a 110 b 410 e 570 f 900 f 1600 f 2500 i 1300 a 640 a

100 26 a 140 b 460 f 590 f 880 g 1400 f 2500 d 1300 b 600 a

150 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

200 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

250 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

300 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

350 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

400 38 a 190 b 550 f 630 g 840 h 1200 g 2000 f 1500 b 560 a

Status

30

bull Draft Document Released to the Federal Register on 72817ndash Public comment period of 60 days ndash Due Date for Comments is 92617

bull Revisions will be made considering public commentsbull Subsequent EPA intra-agency review of final criteria

document will then be conducted bull Publication of final criteria document planned for Late Fall

2017

Federal Register Webpagehttpswwwfederalregistergovdocuments201

707282017-15968request-for-scientific-views-draft-updated-aquatic-life-ambient-water-quality-criteria-for-aluminum

Aluminum Criteria Webpagehttpswwwepagovwqc2017-draft-aquatic-life-

criteria-aluminum-freshwater-documents

31

Status

30

bull Draft Document Released to the Federal Register on 72817ndash Public comment period of 60 days ndash Due Date for Comments is 92617

bull Revisions will be made considering public commentsbull Subsequent EPA intra-agency review of final criteria

document will then be conducted bull Publication of final criteria document planned for Late Fall

2017

Federal Register Webpagehttpswwwfederalregistergovdocuments201

707282017-15968request-for-scientific-views-draft-updated-aquatic-life-ambient-water-quality-criteria-for-aluminum

Aluminum Criteria Webpagehttpswwwepagovwqc2017-draft-aquatic-life-

criteria-aluminum-freshwater-documents

31

Federal Register Webpagehttpswwwfederalregistergovdocuments201

707282017-15968request-for-scientific-views-draft-updated-aquatic-life-ambient-water-quality-criteria-for-aluminum

Aluminum Criteria Webpagehttpswwwepagovwqc2017-draft-aquatic-life-

criteria-aluminum-freshwater-documents

31

Contact Information

Aluminum Criteria Lead Diana EignorEignorDianaepagov

202-566-1143

Kathryn Gallagher Branch Chiefgallagherkathrynepagov

202-564-1398 32