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Epithelium and Junctions
VIBS 443/602
EM 3: region of basal lamina and connective tissue beneath intestinal epithelial absorptive cells
1. Plasma membrane
2. Basal lamina
3. Connective tissue
EM 17; capillary in the lamina propria of the intestine (duodenum); 13,500x
1. Smooth muscle cell
2. Capillary
3. Endothelial cell
Renin granules in Kidney (PAS)
Ileum, monkey (PAS)249
Trachea, monkey
Esophagus and trachea, monkey – glands in trachea
Larynx (Gallego's stain)
SPECIALIZATION OF EPITHELIA
MAINTAIN EXTENSIVE
CONTACTS AMONG CELLS
STRUCTURALLY AND FUNCTIONALLY POLARIZED
JUNCTIONSZONULA OCCLUDENS - TIGHT
JUNCTION (BELT)
ZONULA ADHERENS –
ADHERING BELT
DESMOSOME (MACULA
ADHERENS) - SPOT ATTACHMENT
GAP JUNCTIONS - COMMUNICATION
Terminal bars
32409
EM 6b: Region showing zonula occludens, zonula adherents, and macula adherents junctions between cells.
1. Zonula occludens
2. Zonula adherens
3. DesmosomeEM 6b
EM 6b
EM 6c
EM 4: Continuity between junctional complex and terminal web
1. Terminal web
2. Interdigitation of plasma membrane
3. Terminal bar
EM 4a: Ridges and grooves of zonula occludens visualized by freeze cleave microscopy
1. Zonula occludens
Inside the cell Outside the cell
P = Protoplasmic side E = Ectoplasmic side
EM 4a
EM 2a: Particles and pits of gap junction visualized by freeze cleave microscopy
1. Gap junction
EM 2a
Triad with bile duct Liver & spleen with colloidal carbon, rat
118
EM 2: portion of two cells including gap junction
1. Desmosome
2. Gap junction
3. Rough endoplasmic reticulum
absorptive cells of Ileum, monkey250
Ileum, monkey (PAS)
argentaffin cells of Ileum, monkey
Large intestine or Colon, monkey
EM 4c: intestinal absorptive cell (super nuclear region); 60,000x
1. Budding RER
2. Coated vesicle
3. Golgi
4. Mitochondria
5. Nucleus
6. Plasma membrane
7. Primary lysosome
Endothelial cells lining small blood vessel lumen with smooth muscle cells and fibrolasts in the vessel wall.
1. Lumen
2. Endothelial cell
3. Fibrolast
EM 10a: Endothelial cells lining capillary with pericyte in the vessel wall.
1. Lumen
2. Endothelial cell
3. Tight junction
Skeletal muscle in spermatic cord UT 196
196
UT196 valves in large vein of spermatic cord
195
EM 10f: arteriolar wall; 30,000x
1. Apical caeolae
2. Collagen
3. Dense bodies
4. Elastic fiber
5. Endothelial cell
6. Nuclear pore
7. RER
EM 8h: macrophage in testis; 30,000x.
1. Enlarged basal lamina
2. Heterophagic vacuole
3. Leydig cell cytoplasm
4. Nucleolus
5. Myoid cell
Skin abdomen
Skin, scalp
Skin hand monkey109
Skin hand monkey
Skin111
Skin, foot
Vagina
EM 8g: epidermis; 7,000x. Note desmosomal connections between cells
1. Keratinocyte
2. Desmosome
3. Melanocyte
EM 16: surface mucosa of stomach; 13,500x
1. Granules of surface mucous cells
2. Mitochondria
3. Nuclei
4. Lumen
Urinary bladder, monkey160
Ureter – transitional epithelium and smooth muscle
262
Penis – transitional epithelium and surrounding spongy cavernous of penal urethra
277
Penis - transitional epithelium of penal urethra 277