Upload
portia-dennis
View
16
Download
0
Embed Size (px)
DESCRIPTION
HincII. HincII. XbaI. XbaI. Wild type allele. Probe. Probe. Targeting vector. Neo. XbaI. XbaI. Targeted allele. Neo. p53. +/+. +/+. +/-. +/-. -/-. -/-. mkk7. mkk7. MDM2. +/-. +/-. -/-. -/-. p-ERK. +/-. +/-. -/-. -/-. p-p38. +/-. +/-. -/-. -/-. - PowerPoint PPT Presentation
Citation preview
Penninger Fig. S1
a
actin
mkk7
mkk7 +/+ +/- -/-cb
wild type allele
mkk7 +/+ +/- -/-
neo allele1215
Kbp
Neo
HincII HincII Probe
Wild type allele
Targeting vector
Targeted allele
Neo
XbaI XbaI
XbaIProbe
XbaI
Liver x 200 Whole embryos x 5
p53
p-p38
p-ERK
MDM2
+/-
+/-
+/-
+/-
-/-
-/-
-/-
-/-
+/-
+/-
+/-
+/-
-/-
-/-
-/-
-/-
d
0 hmkk7
+/-
H2O2, 48h 0 h
G1: 55%S: 7.8%
G2/M 37%
mkk7 -/-
H2O2, 48h
G1: 70%S: 12%
G2/M 18%
G1: 67%S: 14%
G2/M 19%
G1: 59%S: 10%
G2/M 31%
0
10
30
40
0 48 0 48(h) after H2O2
mkk7 +/- mkk7 -/-
G2/
M (%
)
20
a
b
UVTNFmkk7 -/-+/+ +/- -/-+/+ +/- -/-+/+ +/-
GST-c-Jun
JNK
26
6986
40
121
52
(kDa)
The full scan for Fig. 2a
The full scan for Fig. 2c
MKK7
-/-+/+ +/- -/-+/+ +/-mkk7pBabe
mkk7
26
6986
40
121184
52
(kDa)
The full scan for Fig. 2d
MKK4
JNK
(kDa)
26
6986
40
121184
52
26
6986
40
52
+/- -/- -/-mkk7+--mkk4
MKK7 -actin
26
6986
40
121
52
pBabe cont
rol
mkk7-/-
mkk
7
mkk7+/+
cont
rol
cont
rol
mkk
7
cont
rol
(kDa)
The full scan for Fig. 3b
Fig. S2
c
d e f
Penninger
526986
40
121184
p53
mkk7 +/+ +/- -/-
Jun D6186
49
121184
mkk7 +/+ +/- -/-
ATF-2
526986
40
121184
mkk7 +/+ +/- -/-
mkk7 +/+ +/- -/-
MKK452
6986
40
121184
p3861
26
86
49
121
mkk7 +/+ +/- -/-
c-Jun
mkk7 +/+ +/- -/-
61
36
86
49
121184
Jun B
61
36
86
49
121184mkk7 +/+ +/- -/-
CycB152
28
69
40
mkk7 +/+ +/- -/-
MAPK
61
26
86
49
121184
mkk7 +/+ +/- -/-
JNK526986
40
121184
mkk7 +/+ +/- -/-
p-c-Jun
mkk7+/+ +/- -/-
61
36
86
49
Cdc2
mkk7 +/+ +/- -/-
61
36
86
49
121
32
Fig. S3Penninger
mkk7+/- mkk7-/-
1 4 7
p16
p21
CyclinD1
Cdc2
1 4 710 10
61
36
86
49
121
32
61
36
86
49
121
32
26
15
32
20
36
26
15
32
20
The full scan for Fig. 4b
The full scan for Fig. 4d
JNKCdc2
mkk7 +/- -/- +/- -/-
GST-c-JunHH1
+/- -/-
61
26
86
49
121 +/- -/-
61
26
86
49
121
Passage No.
mkk7+/- mkk7-/-
cdc2
18S
3 6 8 3 6 8
28S
a
b c
d The full scan for Fig. 4c
The full scan for Fig. 4a
Fig. S4Penninger
The full scan for Fig. 4g
cdc2
mkk7 +/- -/-
actin
+/- -/-
The full scan for Fig. 4f
methgf
+/- -/- +/- -/-
+/- -/-
GST-c-Jun
JNK61
26
86
49
121184
+/- -/-
mkk7
The full scan for Fig. 6a
61
36
49 Actin
Cdc236
49
32
mkk7-/-
Cont
rol
c-Ju
n
The full scan for Fig. 6cThe full scan for Fig. 6b
+/+ +/+c-Jun2 5 Passages
61
36
86
49
121
32
Cdc2
-/-AA -/-AA
P-c-Jun
+/+ -/-AA +/+c-Jun -/-AA
2 5 Passages
61
36
86
49
121
32
28S
wt JunAA
18S
4 6 8 4 6 8 Passages
cdc2
The full scan for Fig. 7d
Actin
c-Jun
61
36
86
49
121
32
61
36
86
49
121
32
pBabe
cont
rol
mkk7-/-
c-Ju
n
mkk7+/+
cont
rol
a c
d e f
b