1
Supplementary Fig. S1 Putative binding protein (%) count ZNF313 (NM_018683) 29.8 36 DAZAP2 (NM_014764) 2.5 3 eEF1A1 (NM_001402) 2.5 3 HRG (NM_000412) 2.5 3 AHSG (NM_001622) 1.7 2 CD14 (NM_001040021) 1.7 2 CLU (NM_203339) 1.7 2 FHR (BC074957) 1.7 2 PCOLCE (BC033205) 1.7 2 RPL36a (NM_001001) 1.7 2 Sel P (BC040075) 1.7 2 Others 51.2 62 Total 100.0 121 a ZNF313 30% (36/121) pGBKT7 Representative ZNF313 clone pGBKT7-XAF1 Yeast two-hybrid assay b 0 1 2 3 0 1 2 3 ZNF ZNF313 (V5) XAF1 Tubulin U2OS/XAF1 T98G CHX (10 M) treatment for 24 h g G. gallus RNF114 D. rerio RNF114 S. salar Zn313 H. sapiens RNF166 H. sapiens RNF138 H. sapiens RNF125 R. norvegicus RNF114 M. musculus ZNF313 X. laevis ZFP313 H. sapiens ZNF313 P. troglodytes RNF114 B. taurus RNF114 C. familiaris RNF114 c f 1 Human 2 Chimp 3 Cattle 4 Dog 5 Rat 6 Mouse 7 Frog 8 Chicken 9 Salmon 10 Zebra fish 1. H. sapiens ZNF313 (NP_061153.1) 99 93 94 89 90 87 72 57 53 2. P. troglodytes RNF114 (NP_001120978.1) 99 92 94 89 89 87 73 57 53 3. B. taurus RNF114 (NP_001019702.1) 95 94 94 89 89 87 70 58 54 4. C. familiaris RNF114 (XP_534453.3) 96 96 96 90 90 87 73 53 53 5. R. norvegicus RNF114 (NP_001001517.1) 93 93 94 96 97 93 71 55 52 6. M. musculus ZFP313 (NP_109668.2) 93 93 95 95 99 96 71 55 53 7. X. laevis ZFP313 (AAT28738.1) 92 91 93 83 97 98 70 52 52 8. G. gallus RNF114 (NP_001001767.2) 80 80 79 82 79 79 80 58 54 9. S. salar Zn313 (NP_001133365.1) 72 72 72 67 69 69 68 71 67 10. D. rerio RNF114 (NP_001001828.2) 71 71 71 71 71 71 70 70 79 Protein homology (%) Protein identity (%) d 0 12 24 36 48 1.0 0.8 0.6 0.4 0.2 0 Hours after CHX addition Relative XAF1 level siControl siZNF313-1 T98G e ZNF313 XAF1 Tubulin siControl siZNF313- 1 siZNF313- 2 siZNF313- 3 T98G

Supplementary Fig. S1

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Putative binding protein. (%). count. ZNF313 (NM_018683). 29.8. 36. DAZAP2 (NM_014764). 2.5. 3. Yeast two-hybrid assay. eEF1A1 (NM_001402). 2.5. 3. HRG (NM_000412). 2.5. 3. AHSG (NM_001622). 1.7. 2. pGBKT7-XAF1. ZNF313 30% (36/121). CD14 (NM_001040021). 1.7. 2. pGBKT7. - PowerPoint PPT Presentation

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Page 1: Supplementary Fig. S1

Supplementary Fig. S1

Putative binding protein (%) count

ZNF313 (NM_018683) 29.8 36

DAZAP2 (NM_014764) 2.5 3

eEF1A1 (NM_001402) 2.5 3

HRG (NM_000412) 2.5 3

AHSG (NM_001622) 1.7 2

CD14 (NM_001040021) 1.7 2

CLU (NM_203339) 1.7 2FHR (BC074957) 1.7 2

PCOLCE (BC033205) 1.7 2

RPL36a (NM_001001) 1.7 2

Sel P (BC040075) 1.7 2Others 51.2 62

Total 100.0 121

a

ZNF31330% (36/121)

pGB

KT7

RepresentativeZNF313 clone

pGB

KT7

-XA

F1

Yeast two-hybrid assay

b

0 1 2 3 0 1 2 3 ZNF313-V5 (g)

ZNF313 (V5)

XAF1

Tubulin

U2OS/XAF1 T98G

CHX (10 M) treatment for 24 h

g

G. gallus RNF114

D. rerio RNF114

S. salar Zn313

H. sapiens RNF166

H. sapiens RNF138

H. sapiens RNF125

R. norvegicus RNF114

M. musculus ZNF313X. laevis ZFP313H. sapiens ZNF313

P. troglodytes RNF114B. taurus RNF114

C. familiaris RNF114

c

f

1Human

2Chimp

3Cattle

4Dog

5Rat

6Mouse

7Frog

8Chicken

9Salmon

10Zebra fish

1. H. sapiens ZNF313(NP_061153.1) 99 93 94 89 90 87 72 57 53

2. P. troglodytes RNF114(NP_001120978.1) 99 92 94 89 89 87 73 57 53

3. B. taurus RNF114(NP_001019702.1) 95 94 94 89 89 87 70 58 54

4. C. familiaris RNF114 (XP_534453.3) 96 96 96 90 90 87 73 53 53

5. R. norvegicus RNF114(NP_001001517.1) 93 93 94 96 97 93 71 55 52

6. M. musculus ZFP313(NP_109668.2) 93 93 95 95 99 96 71 55 53

7. X. laevis ZFP313(AAT28738.1) 92 91 93 83 97 98 70 52 52

8. G. gallus RNF114(NP_001001767.2) 80 80 79 82 79 79 80 58 54

9. S. salar Zn313(NP_001133365.1) 72 72 72 67 69 69 68 71 67

10. D. rerio RNF114(NP_001001828.2) 71 71 71 71 71 71 70 70 79

Protein homology (%)

Prot

ein

iden

tity

(%)

d

0 12 24 36 48 (h)

1.0

0.8

0.6

0.4

0.2

0

Hours after CHX addition

Rel

ativ

e XA

F1 le

vel siControl

siZNF313-1

T98G

e

ZNF313

XAF1

Tubulin

siC

ontr

ol

siZN

F313

-1

siZN

F313

-2

siZN

F313

-3

T98G

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