Figure S1 0 10 20 30 40 50 60 Young iso. Young het. O ld het. % Chimerism Figure S1 Blood chimerism between parabiosed partners. Parabiotic mice were analyzed for the degree of peripheral blood chimerism by flow cytometry. Data are plotted as the mean (+/- SD) percent young partner- derived (Ly5.2 + or GFP + ) blood leukocytes present in the bloodstream of young isochronic or old heterochronic parabionts, or of old partner-derived (Ly5.1 + ) leukocytes present in the blood of young heterochronic parabionts. Flow cytometry was performed as previously described 1,2 . 1. Wagers,A.J., Sherwood,R.I., Christensen,J.L. & Weissman,I.L. Little evidence for developmental plasticity of adult hematopoietic stem cells. Science 297, 2256-2259 (2002). 2. Wright,D.E., Wagers,A.J., Gulati,A.P., Johnson,F.L. & Weissman,I.L. Physiological migration of hematopoietic stem and progenitor cells. Science 294, 1933-1936 (2001).

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Figure S1. Figure S1 Blood chimerism between parabiosed partners. - PowerPoint PPT Presentation

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Figure S1

Young iso. Young het. Old het.

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Figure S1 Blood chimerism between parabiosed partners.

Parabiotic mice were analyzed for the degree of peripheral blood chimerism by flow cytometry. Data are plotted as the mean (+/- SD) percent young partner-derived (Ly5.2+ or GFP+) blood leukocytes present in the bloodstream of young isochronic or old heterochronic parabionts, or of old partner-derived (Ly5.1+) leukocytes present in the blood of young heterochronic parabionts. Flow cytometry was performed as previously described1,2.

1. Wagers,A.J., Sherwood,R.I., Christensen,J.L. & Weissman,I.L. Little evidence for developmental plasticity of adult hematopoietic stem cells. Science 297, 2256-2259 (2002).

2. Wright,D.E., Wagers,A.J., Gulati,A.P., Johnson,F.L. & Weissman,I.L. Physiological migration of hematopoietic stem and progenitor cells. Science 294, 1933-1936 (2001).