Dip the leaves in boiling water to kill all the cells and make it permeabl e Cut the leaves, a small hansdful l excludin g the stems and petiloes , put them into a mortar Add 20 cm of propanoe to the mortar and grind up the leaf extract to make a concentr ated extract Transfer the liquid (only the liquid) into a centrifu ge tube and centrifu ge it for 2-3 min. Do not mix or shake te test tube Keep the extract in the dark and in ice until required Extracting the chlorophyll Separation of the pigments by paper chromatography Set up the chromato graphy tank with petroleu m, cover the tank and leave it to saturate Cut pieces of chromato graphy paper to fit into the tank so that it soesn ´t touch the sides but yes the bbottom Draw a pencil line at a height of 1.5 cm in the middle of the paper. The cross will not be sumerged when it ´s put in the jar Use a pipette to extracto f the pigment solution and place it on the cross, let it dry complete ly and repeat this as many times as possible Attach the chromato graphy paper to the lid so that the paper is suspende d in the solvent and the pigment extract is above the
