Fluorophores Guide - Promega

COMPLETE FLUOROPHORES LIST

UV KIT

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SINGLE TUBE AND MICROPLATE FLUOROMETERS

CONTACT INFORMATION

Fluorophores Guide Promega Fluorometers and Fluorescence Modules or Optical Kits provide targeted sensitivity for fluorescence.

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http://www.promega.com


UV KIT

BLUE KIT

GREEN KIT

RED KIT

CUSTOM KITS


CONTACT INFORMATION

Featured Fluorophores

FLUOROPHORES GUIDE

UV Kit DAPI EBFP Hoechst 33258 Dye 7-hydroxy-4-methylcoumarin Quinine Sulfate 4-methylumbelliferone

Blue Kit 5-FAM Calcein DiO Fluorescein FLUO-3 FLUO-4 EGFP GFP Oregon Green 514 QuantiFluor™ dsDNA QuantiFluor™ ssDNA QuantiFluor™ RNA Rhodamine Green SYBR Gold SYBR Green SYTO 9 SYTOX® Green YFP LIVE/DEAD BacLight Bacterial Viability Kits – Syto 9 LIVE/DEAD Cell-Mediated Cytotoxicity Kit – DIO LIVE/DEAD Viability/Cytotoxicity Kit for Mammalian

cells - Calcein

Green Kit AlExa Fluor 555 Cy3 Ethidium Bromide Ethidium Homodimer-1 Propidium Iodide Resorufin RFP Rhod-2 Rhodamine Red SYTOX Orange TAMRA Texas Red TRITC LIVE/DEAD BacLight Bacterial Viability Kits –

Propidium Iodide LIVE/DEAD Cell-Mediated Cytotoxicity Kit –

Propidium Iodide LIVE/DEAD Viability/Cytotoxicity Kit for

Mammalian cells - Ethidium Homodimer-1

Red Kit Allophycocyanin Cy5 DRAQ5 SYTOX Red

Custom Kit SYTOX Blue wtGFP



UV KIT

BLUE KIT

GREEN KIT

RED KIT

CUSTOM KITS


CONTACT INFORMATION

UV Kit

FLUOROPHORES GUIDE

DAPI EBFP Hoechst 33258 Dye 7-hydroxy-4-methylcoumarin Quinine Sulfate 4-methylumbelliferone



UV KIT

BLUE KIT

GREEN KIT

RED KIT

CUSTOM KITS


CONTACT INFORMATION

DAPI

FLUOROPHORES GUIDE

U S E S : Binds strongly to DNA and is typically used to stain both live and dead cells.

Fluorophore Excitation Fluorophore Emission

Optical Kit Excitation Optical Kit Emission

300 400 500

U V K I T



UV KIT

BLUE KIT

GREEN KIT

RED KIT

CUSTOM KITS


CONTACT INFORMATION

EBFP

FLUOROPHORES GUIDE

U S E S : When the gene encoding for EBFP is inserted into the host genome, the encoded protein enables both the tracking of tagged proteins and their Expression levels.



400 500

U V K I T



UV KIT

BLUE KIT

GREEN KIT

RED KIT

CUSTOM KITS


CONTACT INFORMATION

Hoechst 33258 Dye

FLUOROPHORES GUIDE

U S E S : An intercolating dye that binds strongly to DNA.



400 500 600

U V K I T



UV KIT

BLUE KIT

GREEN KIT

RED KIT

CUSTOM KITS


CONTACT INFORMATION

7-hydroxy-4-methylcoumarin

FLUOROPHORES GUIDE

U S E S : A labeling reagent often used for preparing protein and nucleic acid conjugates of blue fluorescence.



400 500 600 300

U V K I T



UV KIT

BLUE KIT

GREEN KIT

RED KIT

CUSTOM KITS


CONTACT INFORMATION

Quinine Sulfate

FLUOROPHORES GUIDE

U S E S : Reference standard for measuring fluorescence quantum yield.



400 500 600 300

U V K I T



UV KIT

BLUE KIT

GREEN KIT

RED KIT

CUSTOM KITS


CONTACT INFORMATION

4-methyl-umbelliferone

FLUOROPHORES GUIDE

U S E S : Substrate recognized by ß-galactosidase as a means of measuring Reporter gene activity.



400 500 600 300

U V K I T



UV KIT

BLUE KIT

GREEN KIT

RED KIT

CUSTOM KITS


CONTACT INFORMATION

Blue Kit

FLUOROPHORES GUIDE

5-FAM Calcein DiO Fluorescein FLUO-3 FLUO-4 EGFP GFP Oregon Green 514 QuantiFluor™ dsDNA QuantiFluor™ ssDNA QuantiFluor™ RNA Rhodamine Green SYBR Gold SYBR Green SYTO 9 SYTOX® Green YFP LIVE/DEAD BacLight Bacterial Viability Kits – Syto 9 LIVE/DEAD Cell-Mediated Cytotoxicity Kit – DIO LIVE/DEAD Viability/Cytotoxicity Kit for Mammalian cells - Calcein



UV KIT

BLUE KIT

GREEN KIT

RED KIT

CUSTOM KITS


CONTACT INFORMATION

5-FAM

FLUOROPHORES GUIDE

U S E S : A derivitive of Fluorescein that has is used for in situ labeling of peptides, proteins, and nucleotides.



400 500 600

B L U E K I T



UV KIT

BLUE KIT

GREEN KIT

RED KIT

CUSTOM KITS


CONTACT INFORMATION

Calcein

FLUOROPHORES GUIDE

U S E S : A calcium indicator that is often used to determine cell viability.



400 500 600

B L U E K I T



UV KIT

BLUE KIT

GREEN KIT

RED KIT

CUSTOM KITS


CONTACT INFORMATION

DiO

FLUOROPHORES GUIDE

U S E S : Used in cell-cell fusion, cellular adhesion, migration and Live/Dead applications.



400 500 600 700

B L U E K I T



UV KIT

BLUE KIT

GREEN KIT

RED KIT

CUSTOM KITS


CONTACT INFORMATION

Fluorescein

FLUOROPHORES GUIDE

U S E S : Fluorescein is often used for in situ labeling of peptides, proteins, and nucleotides.



400 500 600

B L U E K I T



UV KIT

BLUE KIT

GREEN KIT

RED KIT

CUSTOM KITS


CONTACT INFORMATION

Fluo-3

FLUOROPHORES GUIDE

U S E S : A high affinity Ca++ indicator that is commonly used to measure cell signaling.



400 500 600

B L U E K I T



UV KIT

BLUE KIT

GREEN KIT

RED KIT

CUSTOM KITS


CONTACT INFORMATION

Fluo-4

FLUOROPHORES GUIDE

U S E S : A high affinity Ca++ indicator that is commonly used to measure cell signaling, brighter and more stable than Fluo-3.



400 500 600

B L U E K I T



UV KIT

BLUE KIT

GREEN KIT

RED KIT

CUSTOM KITS


CONTACT INFORMATION

EGFP

FLUOROPHORES GUIDE

U S E S : When the gene encoding for EGFP is inserted into the host genome, the encoded protein enables both the tracking of tagged proteins and their expression levels.



400 500 600

B L U E K I T



UV KIT

BLUE KIT

GREEN KIT

RED KIT

CUSTOM KITS


CONTACT INFORMATION

GFP

FLUOROPHORES GUIDE

U S E S : When the gene encoding for GFP is inserted into the host genome, the encoded protein enables both the tracking of tagged proteins and their expression levels.



400 500 600

B L U E K I T



UV KIT

BLUE KIT

GREEN KIT

RED KIT

CUSTOM KITS


CONTACT INFORMATION

Oregon Green

FLUOROPHORES GUIDE

U S E S : Can be conjugated to antibodies and other proteins.



400 500 600

B L U E K I T



UV KIT

BLUE KIT

GREEN KIT

RED KIT

CUSTOM KITS


CONTACT INFORMATION

QuantiFluor™ dsDNA

FLUOROPHORES GUIDE

U S E S : A dsDNA quantitation reagent used for quantitating DNA concentrations.



400 500 600

B L U E K I T



UV KIT

BLUE KIT

GREEN KIT

RED KIT

CUSTOM KITS


CONTACT INFORMATION

QuantiFluor™ ssDNA

FLUOROPHORES GUIDE

U S E S : A ssDNA quantitation reagent used for quantitating DNA concentrations.



400 500 600

B L U E K I T



UV KIT

BLUE KIT

GREEN KIT

RED KIT

CUSTOM KITS


CONTACT INFORMATION

QuantiFluor™ RNA

FLUOROPHORES GUIDE

U S E S : An RNA quantitation reagent used for quantitating RNA concentrations.



400 500 600

B L U E K I T



UV KIT

BLUE KIT

GREEN KIT

RED KIT

CUSTOM KITS


CONTACT INFORMATION

Rhodamine Green

FLUOROPHORES GUIDE




400 500 600

B L U E K I T



UV KIT

BLUE KIT

GREEN KIT

RED KIT

CUSTOM KITS


CONTACT INFORMATION

SYBR Gold

FLUOROPHORES GUIDE

U S E S : Nucleic acid stain used for detecting dsDNA, ssDNA or RNA.



400 500 600 700

B L U E K I T



UV KIT

BLUE KIT

GREEN KIT

RED KIT

CUSTOM KITS


CONTACT INFORMATION

SYBR Green

FLUOROPHORES GUIDE

U S E S : Used as a dye for the quantification of double stranded DNA.



400 500 600 700

B L U E K I T



UV KIT

BLUE KIT

GREEN KIT

RED KIT

CUSTOM KITS


CONTACT INFORMATION

SYTO 9

FLUOROPHORES GUIDE



400 500 600

B L U E K I T



UV KIT

BLUE KIT

GREEN KIT

RED KIT

CUSTOM KITS


CONTACT INFORMATION

Sytox Green

FLUOROPHORES GUIDE

U S E S : SYTOX® Green is a membrane-impermeant nuclear stain that mark cells with compromised plasma membranes.



400 500 600 700

B L U E K I T



UV KIT

BLUE KIT

GREEN KIT

RED KIT

CUSTOM KITS


CONTACT INFORMATION

YFP

FLUOROPHORES GUIDE

U S E S : A genetic mutant of GFP, when encoded the protein enables both the tracking of tagged proteins and their expression levels.



400 500 600

B L U E K I T



UV KIT

BLUE KIT

GREEN KIT

RED KIT

CUSTOM KITS


CONTACT INFORMATION

Propidium Iodide/Syto 9

FLUOROPHORES GUIDE

L I V E / D E A D ® B A C L I G H T ™ B A C T E R I A L V I A B I L I T Y K I T S

Syto 9 Excitation Syto 9 Emission


400 500 600 700

Propidium Iodide Excitation Propidium Iodide Emission


400 500 600

B L U E K I T



UV KIT

BLUE KIT

GREEN KIT

RED KIT

CUSTOM KITS


CONTACT INFORMATION

DiO/Propidium Iodide

FLUOROPHORES GUIDE

L I V E / D E A D ® C E L L - M E D I A T E D C Y T O T O X I C I T Y K I T ( L 7 0 1 0 )

400 500 600 700



400 500 600 700

DiO Excitation DiO Emission


B L U E K I T



UV KIT

BLUE KIT

GREEN KIT

RED KIT

CUSTOM KITS


CONTACT INFORMATION

Calcein/Ethydium homodimer-1

FLUOROPHORES GUIDE

L I V E / D E A D ® V I A B I L I T Y / C Y T O T O X I C I T Y K I T * F O R M A M M A L I A N C E L L S

Ethydium homodimer-1 Excitation Ethydium homodimer-1 Emission


Calcein Excitation Calcein Emission


400 500 600 700

400 500 600

B L U E K I T



UV KIT

BLUE KIT

GREEN KIT

RED KIT

CUSTOM KITS


CONTACT INFORMATION

Green Kit

FLUOROPHORES GUIDE

AlExa Fluor 555 Cy3 Ethidium Bromide Ethidium Homodimer-1 Propidium Iodide Resorufin RFP Rhod-2 Rhodamine Red SYTOX Orange TAMRA Texas Red TRITC LIVE/DEAD BacLight Bacterial Viability Kits – Propidium Iodide LIVE/DEAD Cell-Mediated Cytotoxicity Kit – Propidium Iodide LIVE/DEAD Viability/Cytotoxicity Kit for Mammalian cells - Ethidium

Homodimer-1



UV KIT

BLUE KIT

GREEN KIT

RED KIT

CUSTOM KITS


CONTACT INFORMATION

Alexafluor 555

FLUOROPHORES GUIDE

U S E S : Can be conjugated to antibodies and other proteins



400 500 600 700

G R E E N K I T



UV KIT

BLUE KIT

GREEN KIT

RED KIT

CUSTOM KITS


CONTACT INFORMATION

Cy3

FLUOROPHORES GUIDE

U S E S : Typically linked to nucleic acids or proteins for various studies including proteomics and translocation studies.



400 500 600 700

G R E E N K I T



UV KIT

BLUE KIT

GREEN KIT

RED KIT

CUSTOM KITS


CONTACT INFORMATION

Ethidium Bromide

FLUOROPHORES GUIDE




400 500 600 800 700

G R E E N K I T



UV KIT

BLUE KIT

GREEN KIT

RED KIT

CUSTOM KITS


CONTACT INFORMATION

Ethidium Homodimer-1

FLUOROPHORES GUIDE



400 500 600 700

G R E E N K I T



UV KIT

BLUE KIT

GREEN KIT

RED KIT

CUSTOM KITS


CONTACT INFORMATION

Propidium Iodide

FLUOROPHORES GUIDE




400 500 600 700

G R E E N K I T



UV KIT

BLUE KIT

GREEN KIT

RED KIT

CUSTOM KITS


CONTACT INFORMATION

Resorufin

FLUOROPHORES GUIDE



U S E S : Fluorogenic substrate used for measuring Cytochrome P450 activity.

500 600 700

G R E E N K I T



UV KIT

BLUE KIT

GREEN KIT

RED KIT

CUSTOM KITS


CONTACT INFORMATION

RFP

FLUOROPHORES GUIDE



U S E S : A spectrally distinct companion or substitute for the green fluorescent protein (GFP).

500 600 700

G R E E N K I T



UV KIT

BLUE KIT

GREEN KIT

RED KIT

CUSTOM KITS


CONTACT INFORMATION

Rhod-2

FLUOROPHORES GUIDE



U S E S : A high affinity Ca++ indicator, suitable for longer term calcium measurements.

400 500 600

G R E E N K I T



UV KIT

BLUE KIT

GREEN KIT

RED KIT

CUSTOM KITS


CONTACT INFORMATION

Rhodamine Red

FLUOROPHORES GUIDE




500 600 700

G R E E N K I T



UV KIT

BLUE KIT

GREEN KIT

RED KIT

CUSTOM KITS


CONTACT INFORMATION

SYTOX Orange

FLUOROPHORES GUIDE



U S E S : SYTOX® Orange is a membrane-impermeant nuclear stain that marks cells with compromised plasma membranes.

400 500 600 700

G R E E N K I T



UV KIT

BLUE KIT

GREEN KIT

RED KIT

CUSTOM KITS


CONTACT INFORMATION

TAMRA

FLUOROPHORES GUIDE



U S E S : Predominantly used for nucleotide labeling.

500 600 700

G R E E N K I T



UV KIT

BLUE KIT

GREEN KIT

RED KIT

CUSTOM KITS


CONTACT INFORMATION

Texas Red

FLUOROPHORES GUIDE




500 600 700

G R E E N K I T



UV KIT

BLUE KIT

GREEN KIT

RED KIT

CUSTOM KITS


CONTACT INFORMATION

TRITC

FLUOROPHORES GUIDE




500 600 700

G R E E N K I T



UV KIT

BLUE KIT

GREEN KIT

RED KIT

CUSTOM KITS


CONTACT INFORMATION

Propidium Iodide/Syto 9

FLUOROPHORES GUIDE

L I V E / D E A D ® B A C L I G H T ™ B A C T E R I A L V I A B I L I T Y K I T S

Syto 9 Excitation Syto 9 Emission


400 500 600 700



400 500 600

G R E E N K I T



UV KIT

BLUE KIT

GREEN KIT

RED KIT

CUSTOM KITS


CONTACT INFORMATION

DiO/Propidium Iodide

FLUOROPHORES GUIDE

L I V E / D E A D ® C E L L - M E D I A T E D C Y T O T O X I C I T Y K I T ( L 7 0 1 0 )

400 500 600 700



400 500 600 700

DiO Excitation DiO Emission


G R E E N K I T



UV KIT

BLUE KIT

GREEN KIT

RED KIT

CUSTOM KITS


CONTACT INFORMATION

Calcein/Ethydium homodimer-1

FLUOROPHORES GUIDE

L I V E / D E A D ® V I A B I L I T Y / C Y T O T O X I C I T Y K I T * F O R M A M M A L I A N C E L L S

Ethydium homodimer-1 Excitation Ethydium homodimer-1 Emission


Calcein Excitation Calcein Emission


400 500 600 700

400 500 600

G R E E N K I T



UV KIT

BLUE KIT

GREEN KIT

RED KIT

CUSTOM KITS


CONTACT INFORMATION

Red Kit

FLUOROPHORES GUIDE

Allophycocyanin Cy5 DRAQ5 SYTOX Red



UV KIT

BLUE KIT

GREEN KIT

RED KIT

CUSTOM KITS


CONTACT INFORMATION

Allophycocyanin

FLUOROPHORES GUIDE

700 500 600



U S E S : Can be conjugated to antibodies and other proteins

R E D K I T



UV KIT

BLUE KIT

GREEN KIT

RED KIT

CUSTOM KITS


CONTACT INFORMATION

Cy5

FLUOROPHORES GUIDE

U S E S : Typically linked to nucleic acids or proteins for various studies including proteomics and RNA translocation studies.

500 800 600 700



R E D K I T



UV KIT

BLUE KIT

GREEN KIT

RED KIT

CUSTOM KITS


CONTACT INFORMATION

DRAQ5

FLUOROPHORES GUIDE

U S E S : DRAQ5™ is a novel far-red fluorescent DNA dye that can be used in LIVE cells in combination with other common fluors, especially GFP fusions & FITC-tags.



500 600 800 700

R E D K I T



UV KIT

BLUE KIT

GREEN KIT

RED KIT

CUSTOM KITS


CONTACT INFORMATION

SYTOX® Red

FLUOROPHORES GUIDE

U S E S : SYTOX® Red is a membrane-impermeant nuclear stain that marks cells with compromised plasma membranes.



500 600 700

R E D K I T



UV KIT

BLUE KIT

GREEN KIT

RED KIT

CUSTOM KITS


CONTACT INFORMATION

Custom Kit

FLUOROPHORES GUIDE

SYTOX Blue wtGFP



UV KIT

BLUE KIT

GREEN KIT

RED KIT

CUSTOM KITS


CONTACT INFORMATION

SYTOX Blue

FLUOROPHORES GUIDE

U S E S : SYTOX® Blue dead cell stain is a high-affinity nucleic acid stain that is used for dead cell staining.



400 500 600 300

C U S T O M K I T



UV KIT

BLUE KIT

GREEN KIT

RED KIT

CUSTOM KITS


CONTACT INFORMATION

wtGFP

FLUOROPHORES GUIDE

U S E S : When the gene encoding for wtGFP is inserted into the host genome, the encoded protein enables both the tracking of tagged proteins and their Expression levels.



400 500 600

C U S T O M K I T



UV KIT

BLUE KIT

GREEN KIT

RED KIT

CUSTOM KITS


CONTACT INFORMATION

Promega Fluorometers

FLUOROPHORES GUIDE

GloMax-Multi+ Microplate Reader

Cat #E8032 or #E9032

GloMax-Multi Microplate Reader

Cat #E7031

GloMax-Multi Jr Single Tube Reader

Cat #E6070

QuantiFluor-P Fluorometer

Cat #E6100 or #E6105

QuantiFluor-ST Fluorometer Cat #E6090

UV

Module Cat #E8051

with UV Kit Installed Ex 365nm

Em 410-460nm

Module Cat #E7051

with UV Kit Installed Ex 365nm

Em 410-460nm

UV Fluorescence Optical Kit

Cat #E6072 Ex 365nm

Em 410-450nm

Instrument Cat #E6105 Ex 365 nm

Em 440-470 nm

Ex 365 nm

Em 440-470 nm

BLUE

Module Cat #E8051

with Blue Kit Installed Ex 490nm

Em 510-570nm

Module Cat #E7051

with Blue Kit Installed Ex 490nm

Em 510-570nm

Blue Fluorescence Optical Kit Cat #E6071 Ex 460nm

Em 515-570nm

Ex 460 nm Em 515–575 nm

Ex 460 nm Em 515–575 nm

GREEN

Module Cat #E8051

with Green Kit Installed Ex 525nm

Em 580-640nm

Module Cat #E7051

with Green Kit Installed Ex 525nm

Em 580-640nm

Green Fluorescence Optical Kit Cat #E6073 Ex 525nm

Em 580-640nm

Instrument Cat #E6100 Ex 525 nm

Em >570 nm Not Available

RED

Module Cat #E8051

with Red Kit Installed Ex 625nm

Em 660-720nm

Module Cat #E7051

with Red Kit Installed Ex 625nm

Em 660-720nm

Red Fluorescence Optical Kit Cat #E6074 Ex 625nm

Em 660-725nm

Not Available Not Available

CUSTOM Contact Us Contact Us Contact Us Not Available Not Available



UV KIT

BLUE KIT

GREEN KIT

RED KIT

CUSTOM KITS


CONTACT INFORMATION

Contact Information

FLUOROPHORES GUIDE

We are available to assist you in finding the right Fluorometers plus Fluorescence Modules or Optical Kits to provide targeted sensitivity for fluorescence. Contact us today.

www.fluorometer.com Fluorometer Customer Representative: 408-636-2400 x119 or x133


http://www.fluorometer.com/

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Fluorophores Guide - Promega